Restriction map for
pF1KE2661
(Circular) (Six-base) MAPSORT of: pF1KE2661.seq Check: 1871 from: 1 to: 5048
pF1KE2661 5048 bp
With 182 enzymes: SgfI *
February 2, 2017 14:35 ..
AccI GT'mk_AC
Cuts at: 791 2086 791
Size: 1295 3753
Acc65I G'GTAC_C
Cuts at: 2064 2064
Size: 5048
AclI AA'CG_TT
Cuts at: 4888 4888
Size: 5048
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1552 1624 1668 2873 3305 4052 4320 1552
Size: 72 44 1205 432 747 268 2280
Fragments arranged by size:
2280 1205 747 432 268 72 44
AflIII A'CryG_T
Cuts at: 2400 3504 4481 2400
Size: 1104 977 2967
Fragments arranged by size:
2967 1104 977
AgeI A'CCGG_T
Cuts at: 3436 3436
Size: 5048
AlwNI CAG_nnn'CTG
Cuts at: 1334 2353 3920 1334
Size: 1019 1567 2462
Fragments arranged by size:
2462 1567 1019
ApaLI G'TGCA_C
Cuts at: 3818 3818
Size: 5048
ApoI r'AATT_y
Cuts at: 936 1009 1204 2052 4401 4657 936
Size: 73 195 848 2349 256 1327
Fragments arranged by size:
2349 1327 848 256 195 73
AseI AT'TA_AT
Cuts at: 20 20
Size: 5048
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5048
AvaI C'yCGr_G
Cuts at: 2068 2068
Size: 5048
BamHI G'GATC_C
Cuts at: 2073 2073
Size: 5048
BanI G'GyrC_C
Cuts at: 2064 2478 2726 2761 2064
Size: 414 248 35 4351
Fragments arranged by size:
4351 414 248 35
BanII G_rGCy'C
Cuts at: 2062 3092 2062
Size: 1030 4018
BbeI G_GCGC'C
Cuts at: 2482 2730 2482
Size: 248 4800
BbsI GAAGACnn'nnnn_
Cuts at: 177 1160 177
Size: 983 4065
BciVI GTATCCnnnnn_n'
Cuts at: 2946 3707 5048 2946
Size: 761 1341 2946
Fragments arranged by size:
2946 1341 761
BclI T'GATC_A
Cuts at: 2568 2568
Size: 5048
BfrBI ATG'CAT
Cuts at: 901 1847 901
Size: 946 4102
BglI GCCn_nnn'nGGC
Cuts at: 1994 1994
Size: 5048
BglII A'GATC_T
Cuts at: 2563 4600 2563
Size: 2037 3011
BlpI GC'TnA_GC
Cuts at: 2160 2160
Size: 5048
Bme1580I G_kGCm'C
Cuts at: 2673 2766 3822 2673
Size: 93 1056 3899
Fragments arranged by size:
3899 1056 93
BmgBI CAC'GTC
Cuts at: 789 789
Size: 5048
BmrI ACTGGGnnnn_n'
Cuts at: 2028 2260 2341 2676 4820 2028
Size: 232 81 335 2144 2256
Fragments arranged by size:
2256 2144 335 232 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1632 1908 3415 1632
Size: 276 1507 3265
Fragments arranged by size:
3265 1507 276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 284
Size: 5048
Bpu10I CC'TnA_GC
Cuts at: 1449 1449
Size: 5048
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1790 1990 2032 2224 3103 3595 3893 1790
Size: 200 42 192 879 492 298 2945
Fragments arranged by size:
2945 879 492 298 200 192 42
BsaI GGTCTCn'nnnn_
Cuts at: 1973 4752 1973
Size: 2779 2269
BsaAI yAC'GTr
Cuts at: 2401 2401
Size: 5048
BsaBI GATnn'nnATC
Cuts at: 6 146 1598 2562 4599 6
Size: 140 1452 964 2037 455
Fragments arranged by size:
2037 1452 964 455 140
BsaHI Gr'CG_yC
Cuts at: 1951 2479 2727 3429 4925 1951
Size: 528 248 702 1496 2074
Fragments arranged by size:
2074 1496 702 528 248
BsaWI w'CCGG_w
Cuts at: 2237 2758 3436 3710 3857 4268 2237
Size: 521 678 274 147 411 3017
Fragments arranged by size:
3017 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 260 290 812 842 103
Size: 30 127 30 522 30 4309
Fragments arranged by size:
4309 522 127 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 281 1445 1498 1553 1608 1884 281
Size: 1164 53 55 55 276 3445
Fragments arranged by size:
3445 1164 276 55 55 53
BseYI C'CCAG_C
Cuts at: 2473 3231 3808 4304 2473
Size: 758 577 496 3217
Fragments arranged by size:
3217 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 769
Size: 5048
BsiEI CG_ry'CG
Cuts at: 104 2590 2636 3844 104
Size: 2486 46 1208 1308
Fragments arranged by size:
2486 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1677 2062 2840 3030 3822 1677
Size: 385 778 190 792 2903
Fragments arranged by size:
2903 792 778 385 190
BsmI GAATG_Cn'
Cuts at: 916 1849 916
Size: 933 4115
Bsp1286I G_dGCh'C
Cuts at: 1677 2062 2673 2766 2840 3030 3092 3822 1677
Size: 385 611 93 74 190 62 730 2903
Fragments arranged by size:
2903 730 611 385 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4268 4268
Size: 5048
BspHI T'CATG_A
Cuts at: 4224 4224
Size: 5048
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2098 2312 2614 2995 2098
Size: 214 302 381 4151
Fragments arranged by size:
4151 381 302 214
BsrBI CCG'CTC
Cuts at: 50 169 3340 3394 4878 5043 50
Size: 119 3171 54 1484 165 55
Fragments arranged by size:
3171 1484 165 119 55 54
BsrDI GCAATG_nn'
Cuts at: 278 974 2960 278
Size: 696 1986 2366
Fragments arranged by size:
2366 1986 696
BsrFI r'CCGG_y
Cuts at: 1045 1096 3046 3227 3436 1045
Size: 51 1950 181 209 2657
Fragments arranged by size:
2657 1950 209 181 51
BsrGI T'GTAC_A
Cuts at: 803 803
Size: 5048
BssSI C'ACGA_G
Cuts at: 1510 3319 3677 1510
Size: 1809 358 2881
Fragments arranged by size:
2881 1809 358
BstAPI GCAn_nnn'nTGC
Cuts at: 907 1672 2425 907
Size: 765 753 3530
Fragments arranged by size:
3530 765 753
BstBI TT'CG_AA
Cuts at: 3409 3409
Size: 5048
BstYI r'GATC_y
Cuts at: 1 586 750 1385 2073 2540 2563 2898 3144
Size: 585 164 635 688 467 23 335 246
Cuts at: 3144 4156 4242 4254 4600 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 688 635 585 467 449 346 335
246 164 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5048
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2998 3179 3342 2998
Size: 181 163 4704
Fragments arranged by size:
4704 181 163
BtsI GCAGTG_nn'
Cuts at: 848 2291 848
Size: 1443 3605
ClaI AT'CG_AT
Cuts at: 240 240
Size: 5048
DraI TTT'AAA
Cuts at: 231 2047 231
Size: 1816 3232
DrdI GACnn_nn'nnGTC
Cuts at: 2754 3612 2754
Size: 858 4190
EaeI y'GGCC_r
Cuts at: 681 767 845 2633 2807 3198 3225 4570 681
Size: 86 78 1788 174 391 27 1345 1159
Fragments arranged by size:
1788 1345 1159 391 174 86 78 27
EagI C'GGCC_G
Cuts at: 2633 2633
Size: 5048
EarI CTCTTCn'nnn_
Cuts at: 556 1129 1687 3071 3281 556
Size: 573 558 1384 210 2323
Fragments arranged by size:
2323 1384 573 558 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1237 3256 3566 3712 4858 1237
Size: 2019 310 146 1146 1427
Fragments arranged by size:
2019 1427 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2060 2060
Size: 5048
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 1552 1624 1668 2873 3305 4052 4320 284
Size: 1268 72 44 1205 432 747 268 1012
Fragments arranged by size:
1268 1205 1012 747 432 268 72 44
EcoNI CCTnn'n_nnAGG
Cuts at: 461 461
Size: 5048
EcoO109I rG'GnC_Cy
Cuts at: 1611 2187 1611
Size: 576 4472
EcoRI G'AATT_C
Cuts at: 2052 2052
Size: 5048
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 585 3067 585
Size: 2482 2566
FspI TGC'GCA
Cuts at: 2829 2829
Size: 5048
HaeII r_GCGC'y
Cuts at: 2482 2730 3752 4731 2482
Size: 248 1022 979 2799
Fragments arranged by size:
2799 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 480 512 1421 1453 1603 1635 2890
Size: 32 345 32 909 32 150 32 1255
Cuts at: 2890 2920 2922 2952 103
Size: 30 2 30 2199
Fragments arranged by size:
2199 1255 909 345 150 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 792 1004 1559 2087 792
Size: 212 555 528 3753
Fragments arranged by size:
3753 555 528 212
HindIII A'AGCT_T
Cuts at: 1937 2008 1937
Size: 71 4977
KasI G'GCGC_C
Cuts at: 2478 2726 2478
Size: 248 4800
KpnI G_GTAC'C
Cuts at: 2068 2068
Size: 5048
MluI A'CGCG_T
Cuts at: 4481 4481
Size: 5048
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3719 3903 3719
Size: 184 4864
MscI TGG'CCA
Cuts at: 683 769 847 2809 683
Size: 86 78 1962 2922
Fragments arranged by size:
2922 1962 86 78
MslI CAynn'nnrTG
Cuts at: 1411 3037 3164 1411
Size: 1626 127 3295
Fragments arranged by size:
3295 1626 127
MspA1I CmG'CkG
Cuts at: 687 1749 2159 2473 2833 3846 4091 687
Size: 1062 410 314 360 1013 245 1644
Fragments arranged by size:
1644 1062 1013 410 360 314 245
NaeI GCC'GGC
Cuts at: 1098 3229 1098
Size: 2131 2917
NarI GG'CG_CC
Cuts at: 2479 2727 2479
Size: 248 4800
NcoI C'CATG_G
Cuts at: 107 107
Size: 5048
NgoMIV G'CCGG_C
Cuts at: 1096 3227 1096
Size: 2131 2917
NsiI A_TGCA'T
Cuts at: 903 1849 903
Size: 946 4102
NspI r_CATG'y
Cuts at: 679 2101 2601 3508 679
Size: 1422 500 907 2219
Fragments arranged by size:
2219 1422 907 500
PciI A'CATG_T
Cuts at: 3504 3504
Size: 5048
PflMI CCAn_nnn'nTGG
Cuts at: 930 930
Size: 5048
PmeI GTTT'AAAC
Cuts at: 2047 2047
Size: 5048
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4187 4219 4187
Size: 32 5016
PpuMI rG'GwC_Cy
Cuts at: 1611 1611
Size: 5048
PsiI TTA'TAA
Cuts at: 289 289
Size: 5048
PstI C_TGCA'G
Cuts at: 1906 2095 2780 1906
Size: 189 685 4174
Fragments arranged by size:
4174 685 189
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5048
PvuII CAG'CTG
Cuts at: 687 2473 2833 687
Size: 1786 360 2902
Fragments arranged by size:
2902 1786 360
RsrII CG'GwC_CG
Cuts at: 3243 3243
Size: 5048
SacI G_AGCT'C
Cuts at: 2062 2062
Size: 5048
SalI G'TCGA_C
Cuts at: 790 2085 790
Size: 1295 3753
SapI GCTCTTCn'nnn_
Cuts at: 1687 3071 3281 1687
Size: 1384 210 3454
Fragments arranged by size:
3454 1384 210
SbfI CC_TGCA'GG
Cuts at: 2095 2095
Size: 5048
ScaI AGT'ACT
Cuts at: 4565 4565
Size: 5048
SfcI C'TryA_G
Cuts at: 33 314 746 1902 2091 2776 3769 3960 33
Size: 281 432 1156 189 685 993 191 1121
Fragments arranged by size:
1156 1121 993 685 432 281 191 189
SfoI GGC'GCC
Cuts at: 2480 2728 2480
Size: 248 4800
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5048
SmaI CCC'GGG
Cuts at: 2070 2070
Size: 5048
SmlI C'TyrA_G
Cuts at: 1805 2005 2011 2203 3118 3610 3872 1805
Size: 200 6 192 915 492 262 2981
Fragments arranged by size:
2981 915 492 262 200 192 6
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5048
SphI G_CATG'C
Cuts at: 2101 2601 2101
Size: 500 4548
StyI C'CwwG_G
Cuts at: 107 884 2182 2536 107
Size: 777 1298 354 2619
Fragments arranged by size:
2619 1298 777 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2631 2764 3223 3432 2631
Size: 133 459 209 4247
Fragments arranged by size:
4247 459 209 133
TatI w'GTAC_w
Cuts at: 803 4563 803
Size: 3760 1288
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 260 292 812 844 4565 4597 260
Size: 32 520 32 3721 32 711
Fragments arranged by size:
3721 711 520 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2845 2845
Size: 5048
XbaI T'CTAG_A
Cuts at: 2079 2079
Size: 5048
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 691 2543 691
Size: 1852 3196
XmaI C'CCGG_G
Cuts at: 2068 2068
Size: 5048
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PpuMI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BmtI BsiWI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI PacI PasI PfoI PmlI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI