Restriction map for
pF1KE2663
(Circular) (Six-base) MAPSORT of: pF1KE2663.seq Check: 6899 from: 1 to: 4586
pF1KE2663 4586 bp
With 182 enzymes: SgfI *
January 20, 2017 09:25 ..
AccI GT'mk_AC
Cuts at: 1624 1624
Size: 4586
Acc65I G'GTAC_C
Cuts at: 1602 1602
Size: 4586
AclI AA'CG_TT
Cuts at: 4426 4426
Size: 4586
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1055 2411 2843 3590 3858 1055
Size: 1356 432 747 268 1783
Fragments arranged by size:
1783 1356 747 432 268
AflIII A'CryG_T
Cuts at: 1938 3042 4019 1938
Size: 1104 977 2505
Fragments arranged by size:
2505 1104 977
AgeI A'CCGG_T
Cuts at: 2974 2974
Size: 4586
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 179 179
Size: 4586
AlwNI CAG_nnn'CTG
Cuts at: 1891 3458 1891
Size: 1567 3019
ApaLI G'TGCA_C
Cuts at: 3356 3356
Size: 4586
ApoI r'AATT_y
Cuts at: 1068 1590 3939 4195 1068
Size: 522 2349 256 1459
Fragments arranged by size:
2349 1459 522 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4586
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4586
AvaI C'yCGr_G
Cuts at: 239 714 747 1606 239
Size: 475 33 859 3219
Fragments arranged by size:
3219 859 475 33
AvrII C'CTAG_G
Cuts at: 1366 1366
Size: 4586
BamHI G'GATC_C
Cuts at: 1611 1611
Size: 4586
BanI G'GyrC_C
Cuts at: 230 1602 2016 2264 2299 230
Size: 1372 414 248 35 2517
Fragments arranged by size:
2517 1372 414 248 35
BanII G_rGCy'C
Cuts at: 706 1016 1600 2630 706
Size: 310 584 1030 2662
Fragments arranged by size:
2662 1030 584 310
BbeI G_GCGC'C
Cuts at: 234 2020 2268 234
Size: 1786 248 2552
Fragments arranged by size:
2552 1786 248
BbsI GAAGACnn'nnnn_
Cuts at: 1251 1424 1251
Size: 173 4413
BbvCI CC'TCA_GC
Cuts at: 190 190
Size: 4586
BciVI GTATCCnnnnn_n'
Cuts at: 2484 3245 4586 2484
Size: 761 1341 2484
Fragments arranged by size:
2484 1341 761
BclI T'GATC_A
Cuts at: 983 2106 983
Size: 1123 3463
BglI GCCn_nnn'nGGC
Cuts at: 228 228
Size: 4586
BglII A'GATC_T
Cuts at: 1501 2101 4138 1501
Size: 600 2037 1949
Fragments arranged by size:
2037 1949 600
BlpI GC'TnA_GC
Cuts at: 1698 1698
Size: 4586
Bme1580I G_kGCm'C
Cuts at: 1445 2211 2304 3360 1445
Size: 766 93 1056 2671
Fragments arranged by size:
2671 1056 766 93
BmrI ACTGGGnnnn_n'
Cuts at: 1010 1798 1879 2214 4358 1010
Size: 788 81 335 2144 1238
Fragments arranged by size:
2144 1238 788 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2953 2953
Size: 4586
Bpu10I CC'TnA_GC
Cuts at: 190 190
Size: 4586
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1762 2641 3133 3431 1762
Size: 879 492 298 2917
Fragments arranged by size:
2917 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 562 4290 562
Size: 3728 858
BsaAI yAC'GTr
Cuts at: 1939 1939
Size: 4586
BsaBI GATnn'nnATC
Cuts at: 6 2100 4137 6
Size: 2094 2037 455
Fragments arranged by size:
2094 2037 455
BsaHI Gr'CG_yC
Cuts at: 231 2017 2265 2967 4463 231
Size: 1786 248 702 1496 354
Fragments arranged by size:
1786 1496 702 354 248
BsaWI w'CCGG_w
Cuts at: 1775 2296 2974 3248 3395 3806 1775
Size: 521 678 274 147 411 2555
Fragments arranged by size:
2555 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 693 723 1156 1186 693
Size: 30 433 30 4093
Fragments arranged by size:
4093 433 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 802 802
Size: 4586
BseYI C'CCAG_C
Cuts at: 2011 2769 3346 3842 2011
Size: 758 577 496 2755
Fragments arranged by size:
2755 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 737 2128 2174 3382 104
Size: 633 1391 46 1208 1308
Fragments arranged by size:
1391 1308 1208 633 46
BsiHKAI G_wGCw'C
Cuts at: 317 706 1600 2378 2568 3360 317
Size: 389 894 778 190 792 1543
Fragments arranged by size:
1543 894 792 778 389 190
BsmI GAATG_Cn'
Cuts at: 468 468
Size: 4586
BsmBI CGTCTCn'nnnn_
Cuts at: 203 203
Size: 4586
Bsp1286I G_dGCh'C
Cuts at: 317 706 1016 1445 1600 2211 2304 2378 2568
Size: 389 310 429 155 611 93 74 190
Cuts at: 2568 2630 3360 317
Size: 62 730 1543
Fragments arranged by size:
1543 730 611 429 389 310 190 155
93 74 62
BspEI T'CCGG_A
Cuts at: 3806 3806
Size: 4586
BspHI T'CATG_A
Cuts at: 549 3762 549
Size: 3213 1373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1636 1850 2152 2533 1636
Size: 214 302 381 3689
Fragments arranged by size:
3689 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2878 2932 4416 4581 50
Size: 2828 54 1484 165 55
Fragments arranged by size:
2828 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 973 2498 973
Size: 1525 3061
BsrFI r'CCGG_y
Cuts at: 233 2584 2765 2974 233
Size: 2351 181 209 1845
Fragments arranged by size:
2351 1845 209 181
BsrGI T'GTAC_A
Cuts at: 659 659
Size: 4586
BssSI C'ACGA_G
Cuts at: 2857 3215 2857
Size: 358 4228
BstAPI GCAn_nnn'nTGC
Cuts at: 465 834 1963 465
Size: 369 1129 3088
Fragments arranged by size:
3088 1129 369
BstBI TT'CG_AA
Cuts at: 768 2947 768
Size: 2179 2407
BstYI r'GATC_y
Cuts at: 1 1501 1611 2078 2101 2436 2682 3694 3780
Size: 1500 110 467 23 335 246 1012 86
Cuts at: 3780 3792 4138 1
Size: 12 346 449
Fragments arranged by size:
1500 1012 467 449 346 335 246 110
86 23 12
BtgI C'CryG_G
Cuts at: 503 782 503
Size: 279 4307
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2536 2717 2880 2536
Size: 181 163 4242
Fragments arranged by size:
4242 181 163
BtsI GCAGTG_nn'
Cuts at: 1829 1829
Size: 4586
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 591 626 591
Size: 35 4551
DraI TTT'AAA
Cuts at: 1585 1585
Size: 4586
DrdI GACnn_nn'nnGTC
Cuts at: 2292 3150 2292
Size: 858 3728
EaeI y'GGCC_r
Cuts at: 140 341 2171 2345 2736 2763 4108 140
Size: 201 1830 174 391 27 1345 618
Fragments arranged by size:
1830 1345 618 391 201 174 27
EagI C'GGCC_G
Cuts at: 2171 2171
Size: 4586
EarI CTCTTCn'nnn_
Cuts at: 2609 2819 2609
Size: 210 4376
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2794 3104 3250 4396 2794
Size: 310 146 1146 2984
Fragments arranged by size:
2984 1146 310 146
EcoICRI GAG'CTC
Cuts at: 704 1598 704
Size: 894 3692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1055 2411 2843 3590 3858 1055
Size: 1356 432 747 268 1783
Fragments arranged by size:
1783 1356 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 150 150
Size: 4586
EcoO109I rG'GnC_Cy
Cuts at: 1315 1725 1315
Size: 410 4176
EcoRI G'AATT_C
Cuts at: 1590 1590
Size: 4586
EcoRV GAT'ATC
Cuts at: 594 594
Size: 4586
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 4586
FspI TGC'GCA
Cuts at: 2367 2367
Size: 4586
HaeII r_GCGC'y
Cuts at: 234 2020 2268 3290 4269 234
Size: 1786 248 1022 979 551
Fragments arranged by size:
1786 1022 979 551 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 302 334 1156 1188 2428 2458 2460 2490 302
Size: 32 822 32 1240 30 2 30 2398
Fragments arranged by size:
2398 1240 822 32 32 30 30 2
HincII GTy'rAC
Cuts at: 186 401 1625 186
Size: 215 1224 3147
Fragments arranged by size:
3147 1224 215
HindIII A'AGCT_T
Cuts at: 1483 1483
Size: 4586
KasI G'GCGC_C
Cuts at: 230 2016 2264 230
Size: 1786 248 2552
Fragments arranged by size:
2552 1786 248
KpnI G_GTAC'C
Cuts at: 1606 1606
Size: 4586
MfeI C'AATT_G
Cuts at: 944 944
Size: 4586
MluI A'CGCG_T
Cuts at: 4019 4019
Size: 4586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1352 3257 3441 1352
Size: 1905 184 2497
Fragments arranged by size:
2497 1905 184
MscI TGG'CCA
Cuts at: 2347 2347
Size: 4586
MslI CAynn'nnrTG
Cuts at: 583 2575 2702 583
Size: 1992 127 2467
Fragments arranged by size:
2467 1992 127
MspA1I CmG'CkG
Cuts at: 917 1697 2011 2371 3384 3629 917
Size: 780 314 360 1013 245 1874
Fragments arranged by size:
1874 1013 780 360 314 245
NaeI GCC'GGC
Cuts at: 235 2767 235
Size: 2532 2054
NarI GG'CG_CC
Cuts at: 231 2017 2265 231
Size: 1786 248 2552
Fragments arranged by size:
2552 1786 248
NcoI C'CATG_G
Cuts at: 503 503
Size: 4586
NgoMIV G'CCGG_C
Cuts at: 233 2765 233
Size: 2532 2054
NspI r_CATG'y
Cuts at: 184 660 1639 2139 3046 184
Size: 476 979 500 907 1724
Fragments arranged by size:
1724 979 907 500 476
PciI A'CATG_T
Cuts at: 3042 3042
Size: 4586
PmeI GTTT'AAAC
Cuts at: 1585 1585
Size: 4586
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 3725
Size: 32 4554
PpuMI rG'GwC_Cy
Cuts at: 1315 1315
Size: 4586
PshAI GACnn'nnGTC
Cuts at: 1313 1313
Size: 4586
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 643 675 643
Size: 32 4554
PstI C_TGCA'G
Cuts at: 1633 2318 1633
Size: 685 3901
PvuI CG_AT'CG
Cuts at: 104 737 104
Size: 633 3953
PvuII CAG'CTG
Cuts at: 2011 2371 2011
Size: 360 4226
RsrII CG'GwC_CG
Cuts at: 2781 2781
Size: 4586
SacI G_AGCT'C
Cuts at: 706 1600 706
Size: 894 3692
SalI G'TCGA_C
Cuts at: 1623 1623
Size: 4586
SanDI GG'GwC_CC
Cuts at: 1315 1315
Size: 4586
SapI GCTCTTCn'nnn_
Cuts at: 2609 2819 2609
Size: 210 4376
SbfI CC_TGCA'GG
Cuts at: 1633 1633
Size: 4586
ScaI AGT'ACT
Cuts at: 4103 4103
Size: 4586
SfcI C'TryA_G
Cuts at: 33 1629 2314 3307 3498 33
Size: 1596 685 993 191 1121
Fragments arranged by size:
1596 1121 993 685 191
SfoI GGC'GCC
Cuts at: 232 2018 2266 232
Size: 1786 248 2552
Fragments arranged by size:
2552 1786 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4586
SmaI CCC'GGG
Cuts at: 241 1608 241
Size: 1367 3219
SmlI C'TyrA_G
Cuts at: 714 1741 2656 3148 3410 714
Size: 1027 915 492 262 1890
Fragments arranged by size:
1890 1027 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4586
SphI G_CATG'C
Cuts at: 1639 2139 1639
Size: 500 4086
StyI C'CwwG_G
Cuts at: 503 1144 1298 1366 1720 2074 503
Size: 641 154 68 354 354 3015
Fragments arranged by size:
3015 641 354 354 154 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2169 2302 2761 2970 2169
Size: 133 459 209 3785
Fragments arranged by size:
3785 459 209 133
TatI w'GTAC_w
Cuts at: 659 1181 4101 659
Size: 522 2920 1144
Fragments arranged by size:
2920 1144 522
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 693 725 1279 1311 4103 4135 693
Size: 32 554 32 2792 32 1144
Fragments arranged by size:
2792 1144 554 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2383 2383
Size: 4586
XbaI T'CTAG_A
Cuts at: 1617 1617
Size: 4586
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2081 2081
Size: 4586
XhoI C'TCGA_G
Cuts at: 714 714
Size: 4586
XmaI C'CCGG_G
Cuts at: 239 1606 239
Size: 1367 3219
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PpuMI PshAI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI BaeI BcgI BfrBI BmgBI BmtI BpmI BsgI
BsiWI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII
FseI FspAI HpaI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PsiI PspOMI PspXI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XmnI ZraI