FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 Query: pF1KE2664, 144 aa 1>>>pF1KE2664 144 - 144 aa - 144 aa Library: human.CCDS.faa 18511270 residues in 32554 sequences Statistics: Expectation_n fit: rho(ln(x))= 4.9125+/-0.000645; mu= 13.8671+/- 0.039 mean_var=57.6413+/-11.900, 0's: 0 Z-trim(109.8): 13 B-trim: 0 in 0/51 Lambda= 0.168930 statistics sampled from 11104 (11112) to 11104 sequences Algorithm: FASTA (3.7 Nov 2010) [optimized] Parameters: BL50 matrix (15:-5), open/ext: -10/-2 ktup: 2, E-join: 1 (0.714), E-opt: 0.2 (0.341), width: 16 Scan time: 1.830 The best scores are: opt bits E(32554) CCDS10917.1 TMEM170A gene_id:124491|Hs108|chr16 ( 144) 939 236.2 5.2e-63 CCDS76900.1 TMEM170A gene_id:124491|Hs108|chr16 ( 106) 644 164.3 1.8e-41 CCDS76901.1 TMEM170A gene_id:124491|Hs108|chr16 ( 121) 543 139.7 5.1e-34 CCDS43425.1 TMEM170B gene_id:100113407|Hs108|chr6 ( 132) 372 98.0 1.9e-21 CCDS76899.1 TMEM170A gene_id:124491|Hs108|chr16 ( 76) 258 70.1 2.8e-13 >>CCDS10917.1 TMEM170A gene_id:124491|Hs108|chr16 (144 aa) initn: 939 init1: 939 opt: 939 Z-score: 1244.8 bits: 236.2 E(32554): 5.2e-63 Smith-Waterman score: 939; 100.0% identity (100.0% similar) in 144 aa overlap (1-144:1-144) 10 20 30 40 50 60 pF1KE2 MEREGSGGSGGSAGLLQQILSLKVVPRVGNGTLCPNSTSLCSFPEMWYGVFLWALVSSLF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS10 MEREGSGGSGGSAGLLQQILSLKVVPRVGNGTLCPNSTSLCSFPEMWYGVFLWALVSSLF 10 20 30 40 50 60 70 80 90 100 110 120 pF1KE2 FHVPAGLLALFTLRHHKYGRFMSVSILLMGIVGPITAGILTSAAIAGVYRAAGKEMIPFE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS10 FHVPAGLLALFTLRHHKYGRFMSVSILLMGIVGPITAGILTSAAIAGVYRAAGKEMIPFE 70 80 90 100 110 120 130 140 pF1KE2 ALTLGTGQTFCVLVVSFLRILATL :::::::::::::::::::::::: CCDS10 ALTLGTGQTFCVLVVSFLRILATL 130 140 >>CCDS76900.1 TMEM170A gene_id:124491|Hs108|chr16 (106 aa) initn: 644 init1: 644 opt: 644 Z-score: 858.2 bits: 164.3 E(32554): 1.8e-41 Smith-Waterman score: 644; 100.0% identity (100.0% similar) in 100 aa overlap (45-144:7-106) 20 30 40 50 60 70 pF1KE2 LLQQILSLKVVPRVGNGTLCPNSTSLCSFPEMWYGVFLWALVSSLFFHVPAGLLALFTLR :::::::::::::::::::::::::::::: CCDS76 MQRAAAEMWYGVFLWALVSSLFFHVPAGLLALFTLR 10 20 30 80 90 100 110 120 130 pF1KE2 HHKYGRFMSVSILLMGIVGPITAGILTSAAIAGVYRAAGKEMIPFEALTLGTGQTFCVLV :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS76 HHKYGRFMSVSILLMGIVGPITAGILTSAAIAGVYRAAGKEMIPFEALTLGTGQTFCVLV 40 50 60 70 80 90 140 pF1KE2 VSFLRILATL :::::::::: CCDS76 VSFLRILATL 100 >>CCDS76901.1 TMEM170A gene_id:124491|Hs108|chr16 (121 aa) initn: 787 init1: 543 opt: 543 Z-score: 724.3 bits: 139.7 E(32554): 5.1e-34 Smith-Waterman score: 745; 84.0% identity (84.0% similar) in 144 aa overlap (1-144:1-121) 10 20 30 40 50 60 pF1KE2 MEREGSGGSGGSAGLLQQILSLKVVPRVGNGTLCPNSTSLCSFPEMWYGVFLWALVSSLF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS76 MEREGSGGSGGSAGLLQQILSLKVVPRVGNGTLCPNSTSLCSFPEMWYGVFLWALVSSLF 10 20 30 40 50 60 70 80 90 100 110 120 pF1KE2 FHVPAGLLALFTLRHHKYGRFMSVSILLMGIVGPITAGILTSAAIAGVYRAAGKEMIPFE ::::::::::::::::::: :::::::::::::::::: CCDS76 FHVPAGLLALFTLRHHKYG-----------------------AAIAGVYRAAGKEMIPFE 70 80 90 130 140 pF1KE2 ALTLGTGQTFCVLVVSFLRILATL :::::::::::::::::::::::: CCDS76 ALTLGTGQTFCVLVVSFLRILATL 100 110 120 >>CCDS43425.1 TMEM170B gene_id:100113407|Hs108|chr6 (132 aa) initn: 391 init1: 371 opt: 372 Z-score: 498.5 bits: 98.0 E(32554): 1.9e-21 Smith-Waterman score: 377; 47.5% identity (72.7% similar) in 139 aa overlap (7-144:5-132) 10 20 30 40 50 pF1KE2 MEREGSGGSGGSAGL-LQQILSLKVVPRVGNGTLCPNSTSLCSFPEMWYGVFLWALVSSL ::. . .: .::.::: . .::. : : :::: .::::: ::: CCDS43 MKAEGGDHSMINLSVQQVLSLWA-----HGTVLRNLT------EMWYWIFLWALFSSL 10 20 30 40 60 70 80 90 100 110 pF1KE2 FFHVPAGLLALFTLRHHKYGRFMSVSILLMGIVGPITAGILTSAAIAGVYRAAGKEMIPF : : ::.: . :..:. :: .:: . .:... .:....::::.::.::.:::.: :. CCDS43 FVHGAAGVLMFVMLQRHRQGRVISVIAVSIGFLASVTGAMITSAAVAGIYRVAGKNMAPL 50 60 70 80 90 100 120 130 140 pF1KE2 EALTLGTGQTFCVLVVSFLRILATL :::. :.::: .:..:: :::::: CCDS43 EALVWGVGQTVLTLIISFSRILATL 110 120 130 >>CCDS76899.1 TMEM170A gene_id:124491|Hs108|chr16 (76 aa) initn: 486 init1: 258 opt: 258 Z-score: 351.9 bits: 70.1 E(32554): 2.8e-13 Smith-Waterman score: 444; 76.8% identity (76.8% similar) in 99 aa overlap (46-144:1-76) 20 30 40 50 60 70 pF1KE2 LQQILSLKVVPRVGNGTLCPNSTSLCSFPEMWYGVFLWALVSSLFFHVPAGLLALFTLRH :::::::::::::::::::::::::::::: CCDS76 MWYGVFLWALVSSLFFHVPAGLLALFTLRH 10 20 30 80 90 100 110 120 130 pF1KE2 HKYGRFMSVSILLMGIVGPITAGILTSAAIAGVYRAAGKEMIPFEALTLGTGQTFCVLVV :::: ::::::::::::::::::::::::::::::::: CCDS76 HKYG-----------------------AAIAGVYRAAGKEMIPFEALTLGTGQTFCVLVV 40 50 60 140 pF1KE2 SFLRILATL ::::::::: CCDS76 SFLRILATL 70 144 residues in 1 query sequences 18511270 residues in 32554 library sequences Tcomplib [36.3.4 Apr, 2011] (8 proc) start: Thu Jan 12 11:37:52 2017 done: Thu Jan 12 11:37:52 2017 Total Scan time: 1.830 Total Display time: -0.020 Function used was FASTA [36.3.4 Apr, 2011]