Restriction map for
pF1KE2672
(Circular) (Six-base) MAPSORT of: pF1KE2672.seq Check: 507 from: 1 to: 5408
pF1KE2672 5408 bp
With 182 enzymes: SgfI *
February 10, 2017 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 920 920
Size: 5408
AccI GT'mk_AC
Cuts at: 2446 2446
Size: 5408
Acc65I G'GTAC_C
Cuts at: 1581 2424 1581
Size: 843 4565
AclI AA'CG_TT
Cuts at: 5248 5248
Size: 5408
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1330 1434 1727 2229 3233 3665 4412 4680 1330
Size: 104 293 502 1004 432 747 268 2058
Fragments arranged by size:
2058 1004 747 502 432 293 268 104
AfeI AGC'GCT
Cuts at: 473 473
Size: 5408
AflIII A'CryG_T
Cuts at: 2760 3864 4841 2760
Size: 1104 977 3327
Fragments arranged by size:
3327 1104 977
AgeI A'CCGG_T
Cuts at: 3796 3796
Size: 5408
AleI CACnn'nnGTG
Cuts at: 2029 2029
Size: 5408
AlwNI CAG_nnn'CTG
Cuts at: 185 339 408 676 921 1002 1101 1149 2713
Size: 154 69 268 245 81 99 48 1564
Cuts at: 2713 4280 185
Size: 1567 1313
Fragments arranged by size:
1567 1564 1313 268 245 154 99 81
69 48
ApaI G_GGCC'C
Cuts at: 318 801 1498 2013 318
Size: 483 697 515 3713
Fragments arranged by size:
3713 697 515 483
ApaLI G'TGCA_C
Cuts at: 442 4178 442
Size: 3736 1672
ApoI r'AATT_y
Cuts at: 2412 4761 5017 2412
Size: 2349 256 2803
Fragments arranged by size:
2803 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5408
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5408
AvaI C'yCGr_G
Cuts at: 1313 1634 1777 2186 2428 1313
Size: 321 143 409 242 4293
Fragments arranged by size:
4293 409 321 242 143
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1573 1606 1573
Size: 33 5375
BamHI G'GATC_C
Cuts at: 2156 2433 2156
Size: 277 5131
BanI G'GyrC_C
Cuts at: 1225 1581 2424 2838 3086 3121 1225
Size: 356 843 414 248 35 3512
Fragments arranged by size:
3512 843 414 356 248 35
BanII G_rGCy'C
Cuts at: 318 554 569 633 801 1312 1498 2013 2194
Size: 236 15 64 168 511 186 515 181
Cuts at: 2194 2422 3452 318
Size: 228 1030 2274
Fragments arranged by size:
2274 1030 515 511 236 228 186 181
168 64 15
BbeI G_GCGC'C
Cuts at: 2842 3090 2842
Size: 248 5160
BbsI GAAGACnn'nnnn_
Cuts at: 2135 2135
Size: 5408
BciVI GTATCCnnnnn_n'
Cuts at: 3306 4067 5408 3306
Size: 761 1341 3306
Fragments arranged by size:
3306 1341 761
BclI T'GATC_A
Cuts at: 2928 2928
Size: 5408
BglI GCCn_nnn'nGGC
Cuts at: 646 646
Size: 5408
BglII A'GATC_T
Cuts at: 2139 2923 4960 2139
Size: 784 2037 2587
Fragments arranged by size:
2587 2037 784
BlpI GC'TnA_GC
Cuts at: 2520 2520
Size: 5408
Bme1580I G_kGCm'C
Cuts at: 318 446 801 935 1498 2013 3033 3126 4182
Size: 128 355 134 563 515 1020 93 1056
Cuts at: 4182 318
Size: 1544
Fragments arranged by size:
1544 1056 1020 563 515 355 134 128
93
BmrI ACTGGGnnnn_n'
Cuts at: 1020 2620 2701 3036 5180 1020
Size: 1600 81 335 2144 1248
Fragments arranged by size:
2144 1600 1248 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1095 3775 1095
Size: 2680 2728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 826 989 2059 2392 523
Size: 303 163 1070 333 3539
Fragments arranged by size:
3539 1070 333 303 163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 1168 1267 2584 3463 3955 4253 406
Size: 762 99 1317 879 492 298 1561
Fragments arranged by size:
1561 1317 879 762 492 298 99
BsaI GGTCTCn'nnnn_
Cuts at: 952 2099 2336 5112 952
Size: 1147 237 2776 1248
Fragments arranged by size:
2776 1248 1147 237
BsaAI yAC'GTr
Cuts at: 2761 2761
Size: 5408
BsaBI GATnn'nnATC
Cuts at: 6 2922 4959 6
Size: 2916 2037 455
Fragments arranged by size:
2916 2037 455
BsaHI Gr'CG_yC
Cuts at: 1159 2839 3087 3789 5285 1159
Size: 1680 248 702 1496 1282
Fragments arranged by size:
1680 1496 1282 702 248
BsaWI w'CCGG_w
Cuts at: 2597 3118 3796 4070 4217 4628 2597
Size: 521 678 274 147 411 3377
Fragments arranged by size:
3377 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 359 467 769 1071 1795 1936 2352 359
Size: 108 302 302 724 141 416 3415
Fragments arranged by size:
3415 724 416 302 302 141 108
BseYI C'CCAG_C
Cuts at: 1221 1605 1857 2232 2387 2833 3591 4168 4664
Size: 384 252 375 155 446 758 577 496
Cuts at: 4664 1221
Size: 1965
Fragments arranged by size:
1965 758 577 496 446 384 375 252
155
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 569 574 569
Size: 5 5403
BsiEI CG_ry'CG
Cuts at: 104 1207 2950 2996 4204 104
Size: 1103 1743 46 1208 1308
Fragments arranged by size:
1743 1308 1208 1103 46
BsiHKAI G_wGCw'C
Cuts at: 446 569 722 2422 3200 3390 4182 446
Size: 123 153 1700 778 190 792 1672
Fragments arranged by size:
1700 1672 792 778 190 153 123
BsmBI CGTCTCn'nnnn_
Cuts at: 119 294 119
Size: 175 5233
Bsp1286I G_dGCh'C
Cuts at: 318 446 554 569 633 722 801 935 1312
Size: 128 108 15 64 89 79 134 377
Cuts at: 1312 1498 2013 2194 2422 3033 3126 3200 3390
Size: 186 515 181 228 611 93 74 190
Cuts at: 3390 3452 4182 318
Size: 62 730 1544
Fragments arranged by size:
1544 730 611 515 377 228 190 186
181 134 128 108 93 89 79 74
64 62 15
BspEI T'CCGG_A
Cuts at: 4628 4628
Size: 5408
BspHI T'CATG_A
Cuts at: 1667 4584 1667
Size: 2917 2491
BspMI ACCTGCnnnn'nnnn_
Cuts at: 347 920 1886 2354 2458 2672 2974 3355 347
Size: 573 966 468 104 214 302 381 2400
Fragments arranged by size:
2400 966 573 468 381 302 214 104
BsrBI CCG'CTC
Cuts at: 50 1992 2043 3700 3754 5238 5403 50
Size: 1942 51 1657 54 1484 165 55
Fragments arranged by size:
1942 1657 1484 165 55 54 51
BsrDI GCAATG_nn'
Cuts at: 1949 3320 1949
Size: 1371 4037
BsrFI r'CCGG_y
Cuts at: 135 213 1161 2147 3406 3587 3796 135
Size: 78 948 986 1259 181 209 1747
Fragments arranged by size:
1747 1259 986 948 209 181 78
BsrGI T'GTAC_A
Cuts at: 872 1241 1889 872
Size: 369 648 4391
Fragments arranged by size:
4391 648 369
BssSI C'ACGA_G
Cuts at: 928 1305 1600 3679 4037 928
Size: 377 295 2079 358 2299
Fragments arranged by size:
2299 2079 377 358 295
BstAPI GCAn_nnn'nTGC
Cuts at: 284 339 1005 1101 2785 284
Size: 55 666 96 1684 2907
Fragments arranged by size:
2907 1684 666 96 55
BstBI TT'CG_AA
Cuts at: 3769 3769
Size: 5408
BstEII G'GTnAC_C
Cuts at: 1539 1539
Size: 5408
BstYI r'GATC_y
Cuts at: 1 356 1145 2078 2139 2156 2433 2900 2923
Size: 355 789 933 61 17 277 467 23
Cuts at: 2923 3258 3504 4516 4602 4614 4960 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 933 789 467 449 355 346 335
277 246 86 61 23 17 12
Bsu36I CC'TnA_GG
Cuts at: 197 1083 1367 2335 197
Size: 886 284 968 3270
Fragments arranged by size:
3270 968 886 284
BtgI C'CryG_G
Cuts at: 107 227 458 512 1544 1808 2027 107
Size: 120 231 54 1032 264 219 3488
Fragments arranged by size:
3488 1032 264 231 219 120 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 579 1905 3358 3539 3702 579
Size: 1326 1453 181 163 2285
Fragments arranged by size:
2285 1453 1326 181 163
BtsI GCAGTG_nn'
Cuts at: 238 586 674 1927 2307 2651 238
Size: 348 88 1253 380 344 2995
Fragments arranged by size:
2995 1253 380 348 344 88
ClaI AT'CG_AT
Cuts at: 1850 1850
Size: 5408
DraI TTT'AAA
Cuts at: 2407 2407
Size: 5408
DraIII CAC_nnn'GTG
Cuts at: 1028 1262 1028
Size: 234 5174
DrdI GACnn_nn'nnGTC
Cuts at: 3114 3972 3114
Size: 858 4550
EaeI y'GGCC_r
Cuts at: 1204 2021 2993 3167 3558 3585 4930 1204
Size: 817 972 174 391 27 1345 1682
Fragments arranged by size:
1682 1345 972 817 391 174 27
EagI C'GGCC_G
Cuts at: 1204 2993 1204
Size: 1789 3619
EarI CTCTTCn'nnn_
Cuts at: 346 3431 3641 346
Size: 3085 210 2113
Fragments arranged by size:
3085 2113 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 673 3616 3926 4072 5218 673
Size: 2943 310 146 1146 863
Fragments arranged by size:
2943 1146 863 310 146
EcoICRI GAG'CTC
Cuts at: 567 2420 567
Size: 1853 3555
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 826 989 1330 1434 1727 2059 2229 2392
Size: 303 163 341 104 293 332 170 163
Cuts at: 2392 3233 3665 4412 4680 523
Size: 841 432 747 268 1251
Fragments arranged by size:
1251 841 747 432 341 332 303 293
268 170 163 163 104
EcoO109I rG'GnC_Cy
Cuts at: 112 201 405 1494 1654 1773 2009 2330 2547
Size: 89 204 1089 160 119 236 321 217
Cuts at: 2547 112
Size: 2973
Fragments arranged by size:
2973 1089 321 236 217 204 160 119
89
EcoRI G'AATT_C
Cuts at: 2412 2412
Size: 5408
EcoRV GAT'ATC
Cuts at: 1377 1377
Size: 5408
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3427 3427
Size: 5408
FseI GG_CCGG'CC
Cuts at: 139 139
Size: 5408
FspI TGC'GCA
Cuts at: 3189 3189
Size: 5408
HaeII r_GCGC'y
Cuts at: 475 2842 3090 4112 5091 475
Size: 2367 248 1022 979 792
Fragments arranged by size:
2367 1022 979 792 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 776 808 1038 1070 1081 1113 1469 1501 2197
Size: 32 230 32 11 32 356 32 696
Cuts at: 2197 2229 3250 3280 3282 3312 776
Size: 32 1021 30 2 30 2872
Fragments arranged by size:
2872 1021 696 356 230 32 32 32
32 32 30 30 11 2
HincII GTy'rAC
Cuts at: 2447 2447
Size: 5408
KasI G'GCGC_C
Cuts at: 2838 3086 2838
Size: 248 5160
KpnI G_GTAC'C
Cuts at: 1585 2428 1585
Size: 843 4565
MfeI C'AATT_G
Cuts at: 2307 2307
Size: 5408
MluI A'CGCG_T
Cuts at: 4841 4841
Size: 5408
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2184 4079 4263 2184
Size: 1895 184 3329
Fragments arranged by size:
3329 1895 184
MscI TGG'CCA
Cuts at: 2023 3169 2023
Size: 1146 4262
MslI CAynn'nnrTG
Cuts at: 988 1549 1666 1954 2029 2032 3397 3524 988
Size: 561 117 288 75 3 1365 127 2872
Fragments arranged by size:
2872 1365 561 288 127 117 75 3
MspA1I CmG'CkG
Cuts at: 1571 2111 2154 2519 2833 3193 4206 4451 1571
Size: 540 43 365 314 360 1013 245 2528
Fragments arranged by size:
2528 1013 540 365 360 314 245 43
NaeI GCC'GGC
Cuts at: 137 215 3589 137
Size: 78 3374 1956
Fragments arranged by size:
3374 1956 78
NarI GG'CG_CC
Cuts at: 2839 3087 2839
Size: 248 5160
NcoI C'CATG_G
Cuts at: 107 227 458 512 1544 1808 107
Size: 120 231 54 1032 264 3707
Fragments arranged by size:
3707 1032 264 231 120 54
NgoMIV G'CCGG_C
Cuts at: 135 213 3587 135
Size: 78 3374 1956
Fragments arranged by size:
3374 1956 78
NspI r_CATG'y
Cuts at: 1221 2461 2961 3868 1221
Size: 1240 500 907 2761
Fragments arranged by size:
2761 1240 907 500
PasI CC'CwG_GG
Cuts at: 810 810
Size: 5408
PciI A'CATG_T
Cuts at: 3864 3864
Size: 5408
PflMI CCAn_nnn'nTGG
Cuts at: 1149 2019 1149
Size: 870 4538
PfoI T'CCnGG_A
Cuts at: 1148 2074 1148
Size: 926 4482
PmeI GTTT'AAAC
Cuts at: 2407 2407
Size: 5408
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4547 4579 4547
Size: 32 5376
PpuMI rG'GwC_Cy
Cuts at: 112 1773 2330 112
Size: 1661 557 3190
Fragments arranged by size:
3190 1661 557
PshAI GACnn'nnGTC
Cuts at: 306 306
Size: 5408
PspOMI G'GGCC_C
Cuts at: 314 797 1494 2009 314
Size: 483 697 515 3713
Fragments arranged by size:
3713 697 515 483
PstI C_TGCA'G
Cuts at: 289 347 917 2455 3140 289
Size: 58 570 1538 685 2557
Fragments arranged by size:
2557 1538 685 570 58
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5408
PvuII CAG'CTG
Cuts at: 2833 3193 2833
Size: 360 5048
RsrII CG'GwC_CG
Cuts at: 3603 3603
Size: 5408
SacI G_AGCT'C
Cuts at: 569 2422 569
Size: 1853 3555
SalI G'TCGA_C
Cuts at: 2445 2445
Size: 5408
SanDI GG'GwC_CC
Cuts at: 2330 2330
Size: 5408
SapI GCTCTTCn'nnn_
Cuts at: 3431 3641 3431
Size: 210 5198
SbfI CC_TGCA'GG
Cuts at: 2455 2455
Size: 5408
ScaI AGT'ACT
Cuts at: 4925 4925
Size: 5408
SexAI A'CCwGG_T
Cuts at: 620 620
Size: 5408
SfcI C'TryA_G
Cuts at: 33 118 285 343 913 2451 3136 4129 4320
Size: 85 167 58 570 1538 685 993 191
Cuts at: 4320 33
Size: 1121
Fragments arranged by size:
1538 1121 993 685 570 191 167 85
58
SfoI GGC'GCC
Cuts at: 2840 3088 2840
Size: 248 5160
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5408
SgrAI Cr'CCGG_yG
Cuts at: 1161 1161
Size: 5408
SmaI CCC'GGG
Cuts at: 1315 2188 2430 1315
Size: 873 242 4293
Fragments arranged by size:
4293 873 242
SmlI C'TyrA_G
Cuts at: 421 1183 1282 2563 3478 3970 4232 421
Size: 762 99 1281 915 492 262 1597
Fragments arranged by size:
1597 1281 915 762 492 262 99
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5408
SphI G_CATG'C
Cuts at: 1221 2461 2961 1221
Size: 1240 500 3668
Fragments arranged by size:
3668 1240 500
StuI AGG'CCT
Cuts at: 2176 2176
Size: 5408
StyI C'CwwG_G
Cuts at: 107 141 163 206 227 245 458 512 1488
Size: 34 22 43 21 18 213 54 976
Cuts at: 1488 1544 1808 2542 2896 107
Size: 56 264 734 354 2619
Fragments arranged by size:
2619 976 734 354 264 213 56 54
43 34 22 21 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1039 1213 2991 3124 3583 3792 1039
Size: 174 1778 133 459 209 2655
Fragments arranged by size:
2655 1778 459 209 174 133
TatI w'GTAC_w
Cuts at: 872 1241 1625 1889 4923 872
Size: 369 384 264 3034 1357
Fragments arranged by size:
3034 1357 384 369 264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 712 744 806 838 1987 2019 2162 2194 4925
Size: 32 62 32 1149 32 143 32 2731
Cuts at: 4925 4957 712
Size: 32 1163
Fragments arranged by size:
2731 1163 1149 143 62 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3205 3205
Size: 5408
XbaI T'CTAG_A
Cuts at: 2439 2439
Size: 5408
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 519 1033 1575 1869 2034 2903 519
Size: 514 542 294 165 869 3024
Fragments arranged by size:
3024 869 542 514 294 165
XmaI C'CCGG_G
Cuts at: 1313 2186 2428 1313
Size: 873 242 4293
Fragments arranged by size:
4293 873 242
XmnI GAAnn'nnTTC
Cuts at: 2318 2318
Size: 5408
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BbvCI
BcgI BfrBI BmgBI BmtI Bpu10I BsaXI BsiWI BsmI
BssHII BstXI BstZ17I CspCI EcoNI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PmlI PsiI
PspXI PsrI SacII SfiI SnaBI SrfI SspI SwaI
XhoI ZraI