(Circular) (Six-base) MAPSORT of: pF1KE2677.seq Check: 5608 from: 1 to: 8573 pF1KE2677 8573 bp With 182 enzymes: SgfI * March 1, 2017 16:47 .. AccI GT'mk_AC Cuts at: 670 5611 670 Size: 4941 3632 Acc65I G'GTAC_C Cuts at: 5589 5589 Size: 8573 AclI AA'CG_TT Cuts at: 8413 8413 Size: 8573 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 167 1754 2126 2608 2702 3715 3925 6398 6830 Size: 1587 372 482 94 1013 210 2473 432 Cuts at: 6830 7577 7845 167 Size: 747 268 895 Fragments arranged by size: 2473 1587 1013 895 747 482 432 372 268 210 94 AfeI AGC'GCT Cuts at: 3551 3551 Size: 8573 AflIII A'CryG_T Cuts at: 2949 5925 7029 8006 2949 Size: 2976 1104 977 3516 Fragments arranged by size: 3516 2976 1104 977 AgeI A'CCGG_T Cuts at: 6961 6961 Size: 8573 AleI CACnn'nnGTG Cuts at: 1118 4414 5041 1118 Size: 3296 627 4650 Fragments arranged by size: 4650 3296 627 AlwNI CAG_nnn'CTG Cuts at: 3477 3927 4837 5878 7445 3477 Size: 450 910 1041 1567 4605 Fragments arranged by size: 4605 1567 1041 910 450 ApaLI G'TGCA_C Cuts at: 7343 7343 Size: 8573 ApoI r'AATT_y Cuts at: 192 226 442 487 910 938 985 1454 1563 Size: 34 216 45 423 28 47 469 109 Cuts at: 1563 1900 1907 2452 2883 2912 3236 3258 3705 Size: 337 7 545 431 29 324 22 447 Cuts at: 3705 3715 4877 5149 5172 5302 5459 5577 7926 Size: 10 1162 272 23 130 157 118 2349 Cuts at: 7926 8182 192 Size: 256 583 Fragments arranged by size: 2349 1162 583 545 469 447 431 423 337 324 272 256 216 157 130 118 109 47 45 34 29 28 23 22 10 7 AseI AT'TA_AT Cuts at: 20 2299 3058 4067 20 Size: 2279 759 1009 4526 Fragments arranged by size: 4526 2279 1009 759 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 8573 AvaI C'yCGr_G Cuts at: 2060 5593 2060 Size: 3533 5040 BamHI G'GATC_C Cuts at: 5598 5598 Size: 8573 BanI G'GyrC_C Cuts at: 5589 6003 6251 6286 5589 Size: 414 248 35 7876 Fragments arranged by size: 7876 414 248 35 BanII G_rGCy'C Cuts at: 1950 5377 5587 6617 1950 Size: 3427 210 1030 3906 Fragments arranged by size: 3906 3427 1030 210 BbeI G_GCGC'C Cuts at: 6007 6255 6007 Size: 248 8325 BbsI GAAGACnn'nnnn_ Cuts at: 286 646 1279 1706 1856 2690 2738 4238 286 Size: 360 633 427 150 834 48 1500 4621 Fragments arranged by size: 4621 1500 834 633 427 360 150 48 BbvCI CC'TCA_GC Cuts at: 4571 4571 Size: 8573 BciVI GTATCCnnnnn_n' Cuts at: 6471 7232 8573 6471 Size: 761 1341 6471 Fragments arranged by size: 6471 1341 761 BclI T'GATC_A Cuts at: 2427 4944 5523 6093 2427 Size: 2517 579 570 4907 Fragments arranged by size: 4907 2517 579 570 BfrBI ATG'CAT Cuts at: 395 395 Size: 8573 BglII A'GATC_T Cuts at: 1047 3936 4203 6088 8125 1047 Size: 2889 267 1885 2037 1495 Fragments arranged by size: 2889 2037 1885 1495 267 BlpI GC'TnA_GC Cuts at: 5685 5685 Size: 8573 Bme1580I G_kGCm'C Cuts at: 6198 6291 7347 6198 Size: 93 1056 7424 Fragments arranged by size: 7424 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1316 5785 5866 6201 8345 1316 Size: 4469 81 335 2144 1544 Fragments arranged by size: 4469 2144 1544 335 81 BmtI G_CTAG'C Cuts at: 752 752 Size: 8573 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3915 6940 3915 Size: 3025 5548 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 268 4096 268 Size: 3828 4745 Bpu10I CC'TnA_GC Cuts at: 423 4571 423 Size: 4148 4425 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 352 1556 1589 2383 3760 4123 4312 4505 5749 Size: 1204 33 794 1377 363 189 193 1244 Cuts at: 5749 6628 7120 7418 352 Size: 879 492 298 1507 Fragments arranged by size: 1507 1377 1244 1204 879 794 492 363 298 193 189 33 BsaI GGTCTCn'nnnn_ Cuts at: 8277 8277 Size: 8573 BsaAI yAC'GTr Cuts at: 3085 5926 3085 Size: 2841 5732 BsaBI GATnn'nnATC Cuts at: 6 4513 6087 8124 6 Size: 4507 1574 2037 455 Fragments arranged by size: 4507 2037 1574 455 BsaHI Gr'CG_yC Cuts at: 6004 6252 6954 8450 6004 Size: 248 702 1496 6127 Fragments arranged by size: 6127 1496 702 248 BsaWI w'CCGG_w Cuts at: 5762 6283 6961 7235 7382 7793 5762 Size: 521 678 274 147 411 6542 Fragments arranged by size: 6542 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1732 1834 3172 5107 1732 Size: 102 1338 1935 5198 Fragments arranged by size: 5198 1935 1338 102 BseYI C'CCAG_C Cuts at: 468 4625 5998 6756 7333 7829 468 Size: 4157 1373 758 577 496 1212 Fragments arranged by size: 4157 1373 1212 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 2974 2974 Size: 8573 BsiEI CG_ry'CG Cuts at: 104 2713 6115 6161 7369 104 Size: 2609 3402 46 1208 1308 Fragments arranged by size: 3402 2609 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 5377 5587 6365 6555 7347 5377 Size: 210 778 190 792 6603 Fragments arranged by size: 6603 792 778 210 190 BsmI GAATG_Cn' Cuts at: 617 5130 617 Size: 4513 4060 Bsp1286I G_dGCh'C Cuts at: 1950 5377 5587 6198 6291 6365 6555 6617 7347 Size: 3427 210 611 93 74 190 62 730 Cuts at: 7347 1950 Size: 3176 Fragments arranged by size: 3427 3176 730 611 210 190 93 74 62 BspEI T'CCGG_A Cuts at: 7793 7793 Size: 8573 BspHI T'CATG_A Cuts at: 222 2990 7749 222 Size: 2768 4759 1046 Fragments arranged by size: 4759 2768 1046 BspMI ACCTGCnnnn'nnnn_ Cuts at: 5623 5837 6139 6520 5623 Size: 214 302 381 7676 Fragments arranged by size: 7676 381 302 214 BsrBI CCG'CTC Cuts at: 50 6865 6919 8403 8568 50 Size: 6815 54 1484 165 55 Fragments arranged by size: 6815 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 760 1529 2502 2717 4362 6485 760 Size: 769 973 215 1645 2123 2848 Fragments arranged by size: 2848 2123 1645 973 769 215 BsrFI r'CCGG_y Cuts at: 6571 6752 6961 6571 Size: 181 209 8183 Fragments arranged by size: 8183 209 181 BsrGI T'GTAC_A Cuts at: 4728 4728 Size: 8573 BssSI C'ACGA_G Cuts at: 1211 1816 6844 7202 1211 Size: 605 5028 358 2582 Fragments arranged by size: 5028 2582 605 358 BstAPI GCAn_nnn'nTGC Cuts at: 3910 5950 3910 Size: 2040 6533 BstBI TT'CG_AA Cuts at: 266 297 5550 6934 266 Size: 31 5253 1384 1905 Fragments arranged by size: 5253 1905 1384 31 BstXI CCAn_nnnn'nTGG Cuts at: 4332 4332 Size: 8573 BstYI r'GATC_y Cuts at: 1 697 1047 3138 3936 4203 4833 4903 5598 Size: 696 350 2091 798 267 630 70 695 Cuts at: 5598 6065 6088 6423 6669 7681 7767 7779 8125 Size: 467 23 335 246 1012 86 12 346 Cuts at: 8125 1 Size: 449 Fragments arranged by size: 2091 1012 798 696 695 630 467 449 350 346 335 267 246 86 70 23 12 BstZ17I GTA'TAC Cuts at: 671 671 Size: 8573 Bsu36I CC'TnA_GG Cuts at: 2567 2567 Size: 8573 BtgI C'CryG_G Cuts at: 107 107 Size: 8573 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 6523 6704 6867 6523 Size: 181 163 8229 Fragments arranged by size: 8229 181 163 BtsI GCAGTG_nn' Cuts at: 5021 5816 5021 Size: 795 7778 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 2079 2114 2079 Size: 35 8538 DraI TTT'AAA Cuts at: 1097 1562 1905 2490 3761 5572 1097 Size: 465 343 585 1271 1811 4098 Fragments arranged by size: 4098 1811 1271 585 465 343 DraIII CAC_nnn'GTG Cuts at: 3571 3571 Size: 8573 DrdI GACnn_nn'nnGTC Cuts at: 542 6279 7137 542 Size: 5737 858 1978 Fragments arranged by size: 5737 1978 858 EaeI y'GGCC_r Cuts at: 4322 5270 6158 6332 6723 6750 8095 4322 Size: 948 888 174 391 27 1345 4800 Fragments arranged by size: 4800 1345 948 888 391 174 27 EagI C'GGCC_G Cuts at: 6158 6158 Size: 8573 EarI CTCTTCn'nnn_ Cuts at: 2156 2219 2309 3324 5065 5071 5382 6596 6806 Size: 63 90 1015 1741 6 311 1214 210 Cuts at: 6806 2156 Size: 3923 Fragments arranged by size: 3923 1741 1214 1015 311 210 90 63 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1515 6781 7091 7237 8383 1515 Size: 5266 310 146 1146 1705 Fragments arranged by size: 5266 1705 1146 310 146 EcoICRI GAG'CTC Cuts at: 5375 5585 5375 Size: 210 8363 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 167 268 1754 2126 2608 2702 3715 3925 4096 Size: 101 1486 372 482 94 1013 210 171 Cuts at: 4096 6398 6830 7577 7845 167 Size: 2302 432 747 268 895 Fragments arranged by size: 2302 1486 1013 895 747 482 432 372 268 210 171 101 94 EcoO109I rG'GnC_Cy Cuts at: 5712 5712 Size: 8573 EcoRI G'AATT_C Cuts at: 487 3236 5577 487 Size: 2749 2341 3483 Fragments arranged by size: 3483 2749 2341 EcoRV GAT'ATC Cuts at: 4718 4806 4718 Size: 88 8485 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 507 3481 3745 3955 6592 507 Size: 2974 264 210 2637 2488 Fragments arranged by size: 2974 2637 2488 264 210 FspI TGC'GCA Cuts at: 6354 6354 Size: 8573 HaeII r_GCGC'y Cuts at: 3553 6007 6255 7277 8256 3553 Size: 2454 248 1022 979 3870 Fragments arranged by size: 3870 2454 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2285 2317 2563 2595 4888 4895 4920 4927 6415 Size: 32 246 32 2293 7 25 7 1488 Cuts at: 6415 6445 6447 6477 2285 Size: 30 2 30 4381 Fragments arranged by size: 4381 2293 1488 246 32 32 30 30 25 7 7 2 HincII GTy'rAC Cuts at: 3803 4934 5612 3803 Size: 1131 678 6764 Fragments arranged by size: 6764 1131 678 HindIII A'AGCT_T Cuts at: 208 208 Size: 8573 HpaI GTT'AAC Cuts at: 3803 3803 Size: 8573 KasI G'GCGC_C Cuts at: 6003 6251 6003 Size: 248 8325 KpnI G_GTAC'C Cuts at: 5593 5593 Size: 8573 MfeI C'AATT_G Cuts at: 170 2936 170 Size: 2766 5807 MluI A'CGCG_T Cuts at: 8006 8006 Size: 8573 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1817 2931 7244 7428 1817 Size: 1114 4313 184 2962 Fragments arranged by size: 4313 2962 1114 184 MscI TGG'CCA Cuts at: 4324 5272 6334 4324 Size: 948 1062 6563 Fragments arranged by size: 6563 1062 948 MslI CAynn'nnrTG Cuts at: 1118 2120 2490 4240 4414 5041 6562 6689 1118 Size: 1002 370 1750 174 627 1521 127 3002 Fragments arranged by size: 3002 1750 1521 1002 627 370 174 127 MspA1I CmG'CkG Cuts at: 3477 4629 5684 5998 6358 7371 7616 3477 Size: 1152 1055 314 360 1013 245 4434 Fragments arranged by size: 4434 1152 1055 1013 360 314 245 NaeI GCC'GGC Cuts at: 6754 6754 Size: 8573 NarI GG'CG_CC Cuts at: 6004 6252 6004 Size: 248 8325 NcoI C'CATG_G Cuts at: 107 107 Size: 8573 NdeI CA'TA_TG Cuts at: 2474 2474 Size: 8573 NgoMIV G'CCGG_C Cuts at: 6752 6752 Size: 8573 NheI G'CTAG_C Cuts at: 748 748 Size: 8573 NsiI A_TGCA'T Cuts at: 397 397 Size: 8573 NspI r_CATG'y Cuts at: 1393 2953 3532 4199 4239 5626 6126 7033 1393 Size: 1560 579 667 40 1387 500 907 2933 Fragments arranged by size: 2933 1560 1387 907 667 579 500 40 PciI A'CATG_T Cuts at: 2949 7029 2949 Size: 4080 4493 PflMI CCAn_nnn'nTGG Cuts at: 1670 1670 Size: 8573 PfoI T'CCnGG_A Cuts at: 1400 1400 Size: 8573 PmeI GTTT'AAAC Cuts at: 5572 5572 Size: 8573 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7712 7744 7712 Size: 32 8541 PsiI TTA'TAA Cuts at: 1042 1042 Size: 8573 PstI C_TGCA'G Cuts at: 5620 6305 5620 Size: 685 7888 PvuI CG_AT'CG Cuts at: 104 104 Size: 8573 PvuII CAG'CTG Cuts at: 3477 4629 5998 6358 3477 Size: 1152 1369 360 5692 Fragments arranged by size: 5692 1369 1152 360 RsrII CG'GwC_CG Cuts at: 6768 6768 Size: 8573 SacI G_AGCT'C Cuts at: 5377 5587 5377 Size: 210 8363 SalI G'TCGA_C Cuts at: 5610 5610 Size: 8573 SapI GCTCTTCn'nnn_ Cuts at: 5382 6596 6806 5382 Size: 1214 210 7149 Fragments arranged by size: 7149 1214 210 SbfI CC_TGCA'GG Cuts at: 5620 5620 Size: 8573 ScaI AGT'ACT Cuts at: 8090 8090 Size: 8573 SfcI C'TryA_G Cuts at: 33 1086 4907 5296 5616 6301 7294 7485 33 Size: 1053 3821 389 320 685 993 191 1121 Fragments arranged by size: 3821 1121 1053 993 685 389 320 191 SfoI GGC'GCC Cuts at: 6005 6253 6005 Size: 248 8325 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 8573 SmaI CCC'GGG Cuts at: 5595 5595 Size: 8573 SmlI C'TyrA_G Cuts at: 331 1571 1604 2060 2362 3775 4138 4291 4520 Size: 1240 33 456 302 1413 363 153 229 Cuts at: 4520 5728 6643 7135 7397 331 Size: 1208 915 492 262 1507 Fragments arranged by size: 1507 1413 1240 1208 915 492 456 363 302 262 229 153 33 SnaBI TAC'GTA Cuts at: 3085 3085 Size: 8573 SpeI A'CTAG_T Cuts at: 67 67 Size: 8573 SphI G_CATG'C Cuts at: 4199 5626 6126 4199 Size: 1427 500 6646 Fragments arranged by size: 6646 1427 500 SspI AAT'ATT Cuts at: 612 1184 1643 612 Size: 572 459 7542 Fragments arranged by size: 7542 572 459 StuI AGG'CCT Cuts at: 180 3316 180 Size: 3136 5437 StyI C'CwwG_G Cuts at: 107 399 455 1895 5060 5707 6061 107 Size: 292 56 1440 3165 647 354 2619 Fragments arranged by size: 3165 2619 1440 647 354 292 56 SwaI ATTT'AAAT Cuts at: 2490 3761 2490 Size: 1271 7302 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2727 5148 6156 6289 6748 6957 2727 Size: 2421 1008 133 459 209 4343 Fragments arranged by size: 4343 2421 1008 459 209 133 TatI w'GTAC_w Cuts at: 4011 4728 8088 4011 Size: 717 3360 4496 Fragments arranged by size: 4496 3360 717 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4944 4976 8090 8122 4944 Size: 32 3114 32 5395 Fragments arranged by size: 5395 3114 32 32 Tth111I GACn'n_nGTC Cuts at: 539 6370 539 Size: 5831 2742 XbaI T'CTAG_A Cuts at: 1683 5604 1683 Size: 3921 4652 XcmI CCAnnnn_n'nnnnTGG Cuts at: 891 6068 891 Size: 5177 3396 XhoI C'TCGA_G Cuts at: 2060 2060 Size: 8573 XmaI C'CCGG_G Cuts at: 5593 5593 Size: 8573 XmnI GAAnn'nnTTC Cuts at: 487 491 3728 5175 487 Size: 4 3237 1447 3885 Fragments arranged by size: 3885 3237 1447 4 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI PciI PflMI PfoI PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BcgI BglI BmgBI BsaXI BsiWI BsmBI BssHII BstEII ClaI EcoNI FseI FspAI NotI NruI PacI PasI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI ZraI