(Circular) (Six-base) MAPSORT of: pF1KE2678.seq Check: 6290 from: 1 to: 7565 pF1KE2678 7565 bp With 182 enzymes: SgfI * July 16, 2019 14:21 .. AatII G_ACGT'C Cuts at: 3928 3928 Size: 7565 AccI GT'mk_AC Cuts at: 1676 3554 4603 1676 Size: 1878 1049 4638 Fragments arranged by size: 4638 1878 1049 Acc65I G'GTAC_C Cuts at: 300 3684 4581 300 Size: 3384 897 3284 Fragments arranged by size: 3384 3284 897 AclI AA'CG_TT Cuts at: 3013 7405 3013 Size: 4392 3173 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1143 1322 1830 2967 3052 3394 3459 4308 5390 Size: 179 508 1137 85 342 65 849 1082 Cuts at: 5390 5822 6569 6837 1143 Size: 432 747 268 1871 Fragments arranged by size: 1871 1137 1082 849 747 508 432 342 268 179 85 65 AfeI AGC'GCT Cuts at: 1773 3469 1773 Size: 1696 5869 AflII C'TTAA_G Cuts at: 1501 1501 Size: 7565 AflIII A'CryG_T Cuts at: 725 869 2576 3044 4121 4917 6021 6998 725 Size: 144 1707 468 1077 796 1104 977 1292 Fragments arranged by size: 1707 1292 1104 1077 977 796 468 144 AgeI A'CCGG_T Cuts at: 3731 5953 3731 Size: 2222 5343 AhdI GACnn_n'nnGTC Cuts at: 1029 1029 Size: 7565 AleI CACnn'nnGTG Cuts at: 3790 4027 3790 Size: 237 7328 AlwNI CAG_nnn'CTG Cuts at: 916 1300 3894 4870 6437 916 Size: 384 2594 976 1567 2044 Fragments arranged by size: 2594 2044 1567 976 384 ApaI G_GGCC'C Cuts at: 3623 4377 3623 Size: 754 6811 ApaLI G'TGCA_C Cuts at: 6335 6335 Size: 7565 ApoI r'AATT_y Cuts at: 1149 1508 3229 4569 6918 7174 1149 Size: 359 1721 1340 2349 256 1540 Fragments arranged by size: 2349 1721 1540 1340 359 256 AseI AT'TA_AT Cuts at: 20 20 Size: 7565 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7565 AvaI C'yCGr_G Cuts at: 305 3807 4585 305 Size: 3502 778 3285 Fragments arranged by size: 3502 3285 778 BamHI G'GATC_C Cuts at: 4590 4590 Size: 7565 BanI G'GyrC_C Cuts at: 300 1347 1868 3684 4581 4995 5243 5278 300 Size: 1047 521 1816 897 414 248 35 2587 Fragments arranged by size: 2587 1816 1047 897 521 414 248 35 BanII G_rGCy'C Cuts at: 186 202 496 938 1058 1642 3116 3623 3961 Size: 16 294 442 120 584 1474 507 338 Cuts at: 3961 4111 4377 4579 5609 186 Size: 150 266 202 1030 2142 Fragments arranged by size: 2142 1474 1030 584 507 442 338 294 266 202 150 120 16 BbeI G_GCGC'C Cuts at: 4999 5247 4999 Size: 248 7317 BbsI GAAGACnn'nnnn_ Cuts at: 2562 2682 2562 Size: 120 7445 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 3316 3350 3316 Size: 34 7531 BciVI GTATCCnnnnn_n' Cuts at: 894 5463 6224 7565 894 Size: 4569 761 1341 894 Fragments arranged by size: 4569 1341 894 761 BclI T'GATC_A Cuts at: 1124 5085 1124 Size: 3961 3604 BfrBI ATG'CAT Cuts at: 2214 2257 2214 Size: 43 7522 BglI GCCn_nnn'nGGC Cuts at: 163 1630 3916 4057 163 Size: 1467 2286 141 3671 Fragments arranged by size: 3671 2286 1467 141 BglII A'GATC_T Cuts at: 5080 7117 5080 Size: 2037 5528 BlpI GC'TnA_GC Cuts at: 4225 4677 4225 Size: 452 7113 Bme1580I G_kGCm'C Cuts at: 238 884 1691 2168 3623 4178 4377 5190 5283 Size: 646 807 477 1455 555 199 813 93 Cuts at: 5283 6339 238 Size: 1056 1464 Fragments arranged by size: 1464 1455 1056 813 807 646 555 477 199 93 BmrI ACTGGGnnnn_n' Cuts at: 878 941 3141 3552 3907 4777 4858 5193 7337 Size: 63 2200 411 355 870 81 335 2144 Cuts at: 7337 878 Size: 1106 Fragments arranged by size: 2200 2144 1106 870 411 355 335 81 63 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3897 5932 3897 Size: 2035 5530 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1360 4494 1360 Size: 3134 4431 Bpu10I CC'TnA_GC Cuts at: 1052 2104 1052 Size: 1052 6513 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 751 4741 5620 6112 6410 751 Size: 3990 879 492 298 1906 Fragments arranged by size: 3990 1906 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 325 2118 3641 4404 7269 325 Size: 1793 1523 763 2865 621 Fragments arranged by size: 2865 1793 1523 763 621 BsaAI yAC'GTr Cuts at: 872 3552 4918 872 Size: 2680 1366 3519 Fragments arranged by size: 3519 2680 1366 BsaBI GATnn'nnATC Cuts at: 6 1319 5079 7116 6 Size: 1313 3760 2037 455 Fragments arranged by size: 3760 2037 1313 455 BsaHI Gr'CG_yC Cuts at: 3925 4348 4996 5244 5946 7442 3925 Size: 423 648 248 702 1496 4048 Fragments arranged by size: 4048 1496 702 648 423 248 BsaWI w'CCGG_w Cuts at: 380 3731 3827 3989 4754 5275 5953 6227 6374 Size: 3351 96 162 765 521 678 274 147 Cuts at: 6374 6785 380 Size: 411 1160 Fragments arranged by size: 3351 1160 765 678 521 411 274 162 147 96 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 277 307 478 508 1207 1237 1595 1625 2734 Size: 30 171 30 699 30 358 30 1109 Cuts at: 2734 2764 2776 2806 3718 3748 3766 3796 277 Size: 30 12 30 912 30 18 30 4046 Fragments arranged by size: 4046 1109 912 699 358 171 30 30 30 30 30 30 30 30 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 486 721 1357 1465 2755 2986 3183 3629 486 Size: 235 636 108 1290 231 197 446 4422 Fragments arranged by size: 4422 1290 636 446 235 231 197 108 BseYI C'CCAG_C Cuts at: 205 695 4990 5748 6325 6821 205 Size: 490 4295 758 577 496 949 Fragments arranged by size: 4295 949 758 577 496 490 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1987 3674 3757 1987 Size: 1687 83 5795 Fragments arranged by size: 5795 1687 83 BsiEI CG_ry'CG Cuts at: 104 1666 2738 3249 3445 5107 5153 6361 104 Size: 1562 1072 511 196 1662 46 1208 1308 Fragments arranged by size: 1662 1562 1308 1208 1072 511 196 46 BsiHKAI G_wGCw'C Cuts at: 186 1040 2918 3551 4150 4579 5357 5547 6339 Size: 854 1878 633 599 429 778 190 792 Cuts at: 6339 186 Size: 1412 Fragments arranged by size: 1878 1412 854 792 778 633 599 429 190 BsmI GAATG_Cn' Cuts at: 2934 2934 Size: 7565 BsmBI CGTCTCn'nnnn_ Cuts at: 1818 1818 Size: 7565 Bsp1286I G_dGCh'C Cuts at: 186 202 238 496 884 938 1040 1058 1642 Size: 16 36 258 388 54 102 18 584 Cuts at: 1642 1691 2168 2918 3116 3551 3623 3961 4111 Size: 49 477 750 198 435 72 338 150 Cuts at: 4111 4150 4178 4377 4579 5190 5283 5357 5547 Size: 39 28 199 202 611 93 74 190 Cuts at: 5547 5609 6339 186 Size: 62 730 1412 Fragments arranged by size: 1412 750 730 611 584 477 435 388 338 258 202 199 198 190 150 102 93 74 72 62 54 49 39 36 28 18 16 BspEI T'CCGG_A Cuts at: 6785 6785 Size: 7565 BspHI T'CATG_A Cuts at: 1613 2066 2531 6741 1613 Size: 453 465 4210 2437 Fragments arranged by size: 4210 2437 465 453 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1399 3032 3696 4043 4615 4829 5131 5512 1399 Size: 1633 664 347 572 214 302 381 3452 Fragments arranged by size: 3452 1633 664 572 381 347 302 214 BsrBI CCG'CTC Cuts at: 50 121 3278 3452 3579 5857 5911 7395 7560 Size: 71 3157 174 127 2278 54 1484 165 Cuts at: 7560 50 Size: 55 Fragments arranged by size: 3157 2278 1484 174 165 127 71 55 54 BsrDI GCAATG_nn' Cuts at: 215 1737 2257 2396 2649 3339 4521 5477 215 Size: 1522 520 139 253 690 1182 956 2303 Fragments arranged by size: 2303 1522 1182 956 690 520 253 139 BsrFI r'CCGG_y Cuts at: 3074 3731 3903 4004 4392 5563 5744 5953 3074 Size: 657 172 101 388 1171 181 209 4686 Fragments arranged by size: 4686 1171 657 388 209 181 172 101 BssSI C'ACGA_G Cuts at: 229 268 1081 5836 6194 229 Size: 39 813 4755 358 1600 Fragments arranged by size: 4755 1600 813 358 39 BstAPI GCAn_nnn'nTGC Cuts at: 3749 4942 3749 Size: 1193 6372 BstBI TT'CG_AA Cuts at: 5926 5926 Size: 7565 BstEII G'GTnAC_C Cuts at: 1548 2352 1548 Size: 804 6761 BstXI CCAn_nnnn'nTGG Cuts at: 891 1998 2098 2829 3088 891 Size: 1107 100 731 259 5368 Fragments arranged by size: 5368 1107 731 259 100 BstYI r'GATC_y Cuts at: 1 386 437 662 752 4590 5057 5080 5415 Size: 385 51 225 90 3838 467 23 335 Cuts at: 5415 5661 6673 6759 6771 7117 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 3838 1012 467 449 385 346 335 246 225 90 86 51 23 12 BtgI C'CryG_G Cuts at: 296 486 1820 2591 2724 4302 4334 296 Size: 190 1334 771 133 1578 32 3527 Fragments arranged by size: 3527 1578 1334 771 190 133 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 414 5515 5696 5859 414 Size: 5101 181 163 2120 Fragments arranged by size: 5101 2120 181 163 BtsI GCAGTG_nn' Cuts at: 1010 1640 2045 2315 2370 2768 3099 4178 4808 Size: 630 405 270 55 398 331 1079 630 Cuts at: 4808 1010 Size: 3767 Fragments arranged by size: 3767 1079 630 630 405 398 331 270 55 ClaI AT'CG_AT Cuts at: 3107 3107 Size: 7565 DraI TTT'AAA Cuts at: 4564 4564 Size: 7565 DraIII CAC_nnn'GTG Cuts at: 3672 3672 Size: 7565 DrdI GACnn_nn'nnGTC Cuts at: 5271 6129 5271 Size: 858 6707 EaeI y'GGCC_r Cuts at: 155 164 251 836 2036 2588 2819 3258 3392 Size: 9 87 585 1200 552 231 439 134 Cuts at: 3392 5150 5324 5715 5742 7087 155 Size: 1758 174 391 27 1345 633 Fragments arranged by size: 1758 1345 1200 633 585 552 439 391 231 174 134 87 27 9 EagI C'GGCC_G Cuts at: 5150 5150 Size: 7565 EarI CTCTTCn'nnn_ Cuts at: 190 1081 1298 1612 2986 5588 5798 190 Size: 891 217 314 1374 2602 210 1957 Fragments arranged by size: 2602 1957 1374 891 314 217 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 5773 6083 6229 7375 5773 Size: 310 146 1146 5963 Fragments arranged by size: 5963 1146 310 146 EcoICRI GAG'CTC Cuts at: 184 4577 184 Size: 4393 3172 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1143 1322 1360 1830 2967 3052 3394 3459 4308 Size: 179 38 470 1137 85 342 65 849 Cuts at: 4308 4494 5390 5822 6569 6837 1143 Size: 186 896 432 747 268 1871 Fragments arranged by size: 1871 1137 896 849 747 470 432 342 268 186 179 85 65 38 EcoNI CCTnn'n_nnAGG Cuts at: 1342 1395 4039 1342 Size: 53 2644 4868 Fragments arranged by size: 4868 2644 53 EcoO109I rG'GnC_Cy Cuts at: 711 1622 3619 3620 4374 4704 711 Size: 911 1997 1 754 330 3572 Fragments arranged by size: 3572 1997 911 754 330 1 EcoRI G'AATT_C Cuts at: 4569 4569 Size: 7565 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3489 5584 3489 Size: 2095 5470 FspI TGC'GCA Cuts at: 2815 5346 2815 Size: 2531 5034 HaeII r_GCGC'y Cuts at: 1775 2430 2748 2870 3471 4999 5247 6269 7248 Size: 655 318 122 601 1528 248 1022 979 Cuts at: 7248 1775 Size: 2092 Fragments arranged by size: 2092 1528 1022 979 655 601 318 248 122 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 358 390 511 543 744 776 1015 1047 1595 Size: 32 121 32 201 32 239 32 548 Cuts at: 1595 1627 1648 1680 2734 2766 2932 2964 3718 Size: 32 21 32 1054 32 166 32 754 Cuts at: 3718 3750 5407 5437 5439 5469 358 Size: 32 1657 30 2 30 2454 Fragments arranged by size: 2454 1657 1054 754 548 239 201 166 121 32 32 32 32 32 32 32 32 32 30 30 21 2 HincII GTy'rAC Cuts at: 1677 4604 1677 Size: 2927 4638 HindIII A'AGCT_T Cuts at: 566 566 Size: 7565 KasI G'GCGC_C Cuts at: 4995 5243 4995 Size: 248 7317 KpnI G_GTAC'C Cuts at: 304 3688 4585 304 Size: 3384 897 3284 Fragments arranged by size: 3384 3284 897 MfeI C'AATT_G Cuts at: 554 554 Size: 7565 MluI A'CGCG_T Cuts at: 6998 6998 Size: 7565 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 266 2670 2819 2907 4487 6236 6420 266 Size: 2404 149 88 1580 1749 184 1411 Fragments arranged by size: 2404 1749 1580 1411 184 149 88 MscI TGG'CCA Cuts at: 253 838 2038 3260 5326 253 Size: 585 1200 1222 2066 2492 Fragments arranged by size: 2492 2066 1222 1200 585 MslI CAynn'nnrTG Cuts at: 229 1633 3790 4027 4526 5554 5681 229 Size: 1404 2157 237 499 1028 127 2113 Fragments arranged by size: 2157 2113 1404 1028 499 237 127 MspA1I CmG'CkG Cuts at: 2544 2717 3030 3611 3680 3758 4138 4676 4990 Size: 173 313 581 69 78 380 538 314 Cuts at: 4990 5350 6363 6608 2544 Size: 360 1013 245 3501 Fragments arranged by size: 3501 1013 581 538 380 360 314 313 245 173 78 69 NaeI GCC'GGC Cuts at: 3905 4006 5746 3905 Size: 101 1740 5724 Fragments arranged by size: 5724 1740 101 NarI GG'CG_CC Cuts at: 4996 5244 4996 Size: 248 7317 NcoI C'CATG_G Cuts at: 2591 2724 4302 2591 Size: 133 1578 5854 Fragments arranged by size: 5854 1578 133 NdeI CA'TA_TG Cuts at: 951 951 Size: 7565 NgoMIV G'CCGG_C Cuts at: 3903 4004 5744 3903 Size: 101 1740 5724 Fragments arranged by size: 5724 1740 101 NsiI A_TGCA'T Cuts at: 2216 2259 2216 Size: 43 7522 NspI r_CATG'y Cuts at: 729 1029 2218 2580 4125 4224 4525 4618 5118 Size: 300 1189 362 1545 99 301 93 500 Cuts at: 5118 6025 729 Size: 907 2269 Fragments arranged by size: 2269 1545 1189 907 500 362 301 300 99 93 PasI CC'CwG_GG Cuts at: 4493 4493 Size: 7565 PciI A'CATG_T Cuts at: 725 2576 4121 6021 725 Size: 1851 1545 1900 2269 Fragments arranged by size: 2269 1900 1851 1545 PflMI CCAn_nnn'nTGG Cuts at: 143 396 2730 143 Size: 253 2334 4978 Fragments arranged by size: 4978 2334 253 PmeI GTTT'AAAC Cuts at: 4564 4564 Size: 7565 PmlI CAC'GTG Cuts at: 872 872 Size: 7565 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2998 3030 6704 6736 2998 Size: 32 3674 32 3827 Fragments arranged by size: 3827 3674 32 32 PpuMI rG'GwC_Cy Cuts at: 711 711 Size: 7565 PshAI GACnn'nnGTC Cuts at: 2889 2889 Size: 7565 PsiI TTA'TAA Cuts at: 1862 1862 Size: 7565 PspOMI G'GGCC_C Cuts at: 3619 4373 3619 Size: 754 6811 PstI C_TGCA'G Cuts at: 1262 2453 3029 3890 4040 4612 5297 1262 Size: 1191 576 861 150 572 685 3530 Fragments arranged by size: 3530 1191 861 685 576 572 150 PvuI CG_AT'CG Cuts at: 104 1666 2738 104 Size: 1562 1072 4931 Fragments arranged by size: 4931 1562 1072 PvuII CAG'CTG Cuts at: 2544 2717 3030 3758 4138 4990 5350 2544 Size: 173 313 728 380 852 360 4759 Fragments arranged by size: 4759 852 728 380 360 313 173 RsrII CG'GwC_CG Cuts at: 3734 5760 3734 Size: 2026 5539 SacI G_AGCT'C Cuts at: 186 4579 186 Size: 4393 3172 SalI G'TCGA_C Cuts at: 1675 4602 1675 Size: 2927 4638 SapI GCTCTTCn'nnn_ Cuts at: 190 1298 5588 5798 190 Size: 1108 4290 210 1957 Fragments arranged by size: 4290 1957 1108 210 SbfI CC_TGCA'GG Cuts at: 3890 4612 3890 Size: 722 6843 ScaI AGT'ACT Cuts at: 1270 1843 7082 1270 Size: 573 5239 1753 Fragments arranged by size: 5239 1753 573 SexAI A'CCwGG_T Cuts at: 1544 2090 1544 Size: 546 7019 SfcI C'TryA_G Cuts at: 33 1258 2449 2712 3025 3252 3886 4036 4608 Size: 1225 1191 263 313 227 634 150 572 Cuts at: 4608 5293 6286 6477 33 Size: 685 993 191 1121 Fragments arranged by size: 1225 1191 1121 993 685 634 572 313 263 227 191 150 SfiI GGCCn_nnn'nGGCC Cuts at: 163 163 Size: 7565 SfoI GGC'GCC Cuts at: 4997 5245 4997 Size: 248 7317 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7565 SmaI CCC'GGG Cuts at: 307 4587 307 Size: 4280 3285 SmlI C'TyrA_G Cuts at: 766 1501 4720 5635 6127 6389 766 Size: 735 3219 915 492 262 1942 Fragments arranged by size: 3219 1942 915 735 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 7565 SphI G_CATG'C Cuts at: 2218 4618 5118 2218 Size: 2400 500 4665 Fragments arranged by size: 4665 2400 500 SspI AAT'ATT Cuts at: 2190 2190 Size: 7565 StyI C'CwwG_G Cuts at: 806 2591 2724 3694 3971 4302 4699 5053 806 Size: 1785 133 970 277 331 397 354 3318 Fragments arranged by size: 3318 1785 970 397 354 331 277 133 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 349 1093 1773 2408 2650 3160 3577 5148 5281 Size: 744 680 635 242 510 417 1571 133 Cuts at: 5281 5740 5949 349 Size: 459 209 1965 Fragments arranged by size: 1965 1571 744 680 635 510 459 417 242 209 133 TatI w'GTAC_w Cuts at: 1268 1841 7080 1268 Size: 573 5239 1753 Fragments arranged by size: 5239 1753 573 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 277 309 478 510 1207 1239 2132 2164 3766 Size: 32 169 32 697 32 893 32 1602 Cuts at: 3766 3798 4276 4308 7082 7114 277 Size: 32 478 32 2774 32 728 Fragments arranged by size: 2774 1602 893 728 697 478 169 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 3604 4249 5362 3604 Size: 645 1113 5807 Fragments arranged by size: 5807 1113 645 XbaI T'CTAG_A Cuts at: 4596 4596 Size: 7565 XcmI CCAnnnn_n'nnnnTGG Cuts at: 144 2413 4392 5060 144 Size: 2269 1979 668 2649 Fragments arranged by size: 2649 2269 1979 668 XmaI C'CCGG_G Cuts at: 305 4585 305 Size: 4280 3285 XmnI GAAnn'nnTTC Cuts at: 2980 2980 Size: 7565 ZraI GAC'GTC Cuts at: 3926 3926 Size: 7565 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI AlfI AloI AscI AvrII BaeI BbvCI BmgBI BmtI BsiWI BsrGI BssHII BstZ17I Bsu36I CspCI EcoRV FseI FspAI HpaI NheI NotI NruI PacI PfoI PspXI PsrI SacII SanDI SgrAI SnaBI SrfI StuI SwaI XhoI