Restriction map for
pF1KE2684
(Circular) (Six-base) MAPSORT of: pF1KE2684.seq Check: 8793 from: 1 to: 3764
pF1KE2684 3764 bp
With 182 enzymes: SgfI *
April 21, 2017 11:06 ..
AccI GT'mk_AC
Cuts at: 653 802 653
Size: 149 3615
Acc65I G'GTAC_C
Cuts at: 780 780
Size: 3764
AclI AA'CG_TT
Cuts at: 3604 3604
Size: 3764
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1589 2021 2768 3036 1589
Size: 432 747 268 2317
Fragments arranged by size:
2317 747 432 268
AflIII A'CryG_T
Cuts at: 1116 2220 3197 1116
Size: 1104 977 1683
Fragments arranged by size:
1683 1104 977
AgeI A'CCGG_T
Cuts at: 2152 2152
Size: 3764
AleI CACnn'nnGTG
Cuts at: 510 510
Size: 3764
AlwNI CAG_nnn'CTG
Cuts at: 1069 2636 1069
Size: 1567 2197
ApaLI G'TGCA_C
Cuts at: 2534 2534
Size: 3764
ApoI r'AATT_y
Cuts at: 768 3117 3373 768
Size: 2349 256 1159
Fragments arranged by size:
2349 1159 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3764
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
AvaI C'yCGr_G
Cuts at: 784 784
Size: 3764
BamHI G'GATC_C
Cuts at: 789 789
Size: 3764
BanI G'GyrC_C
Cuts at: 780 1194 1442 1477 780
Size: 414 248 35 3067
Fragments arranged by size:
3067 414 248 35
BanII G_rGCy'C
Cuts at: 330 778 1808 330
Size: 448 1030 2286
Fragments arranged by size:
2286 1030 448
BbeI G_GCGC'C
Cuts at: 1198 1446 1198
Size: 248 3516
BbsI GAAGACnn'nnnn_
Cuts at: 323 323
Size: 3764
BciVI GTATCCnnnnn_n'
Cuts at: 1662 2423 3764 1662
Size: 761 1341 1662
Fragments arranged by size:
1662 1341 761
BclI T'GATC_A
Cuts at: 1284 1284
Size: 3764
BglII A'GATC_T
Cuts at: 1279 3316 1279
Size: 2037 1727
BlpI GC'TnA_GC
Cuts at: 876 876
Size: 3764
Bme1580I G_kGCm'C
Cuts at: 240 1389 1482 2538 240
Size: 1149 93 1056 1466
Fragments arranged by size:
1466 1149 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 976 1057 1392 3536 976
Size: 81 335 2144 1204
Fragments arranged by size:
2144 1204 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 3764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 1819 2311 2609 940
Size: 879 492 298 2095
Fragments arranged by size:
2095 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3468 3468
Size: 3764
BsaAI yAC'GTr
Cuts at: 1117 1117
Size: 3764
BsaBI GATnn'nnATC
Cuts at: 6 1278 3315 6
Size: 1272 2037 455
Fragments arranged by size:
2037 1272 455
BsaHI Gr'CG_yC
Cuts at: 1195 1443 2145 3641 1195
Size: 248 702 1496 1318
Fragments arranged by size:
1496 1318 702 248
BsaWI w'CCGG_w
Cuts at: 123 953 1474 2152 2426 2573 2984 123
Size: 830 521 678 274 147 411 903
Fragments arranged by size:
903 830 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 123 153 123
Size: 30 3734
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 178
Size: 3764
BseYI C'CCAG_C
Cuts at: 1189 1947 2524 3020 1189
Size: 758 577 496 1933
Fragments arranged by size:
1933 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1306 1352 2560 104
Size: 1202 46 1208 1308
Fragments arranged by size:
1308 1208 1202 46
BsiHKAI G_wGCw'C
Cuts at: 778 1556 1746 2538 778
Size: 778 190 792 2004
Fragments arranged by size:
2004 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 465 465
Size: 3764
Bsp1286I G_dGCh'C
Cuts at: 240 330 778 1389 1482 1556 1746 1808 2538
Size: 90 448 611 93 74 190 62 730
Cuts at: 2538 240
Size: 1466
Fragments arranged by size:
1466 730 611 448 190 93 90 74
62
BspEI T'CCGG_A
Cuts at: 2984 2984
Size: 3764
BspHI T'CATG_A
Cuts at: 2940 2940
Size: 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 293 814 1028 1330 1711 293
Size: 521 214 302 381 2346
Fragments arranged by size:
2346 521 381 302 214
BsrBI CCG'CTC
Cuts at: 50 728 2056 2110 3594 3759 50
Size: 678 1328 54 1484 165 55
Fragments arranged by size:
1484 1328 678 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1676 1676
Size: 3764
BsrFI r'CCGG_y
Cuts at: 1762 1943 2152 1762
Size: 181 209 3374
Fragments arranged by size:
3374 209 181
BsrGI T'GTAC_A
Cuts at: 332 332
Size: 3764
BssSI C'ACGA_G
Cuts at: 2035 2393 2035
Size: 358 3406
BstAPI GCAn_nnn'nTGC
Cuts at: 1141 1141
Size: 3764
BstBI TT'CG_AA
Cuts at: 2125 2125
Size: 3764
BstYI r'GATC_y
Cuts at: 1 789 1256 1279 1614 1860 2872 2958 2970
Size: 788 467 23 335 246 1012 86 12
Cuts at: 2970 3316 1
Size: 346 449
Fragments arranged by size:
1012 788 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 654 654
Size: 3764
BtgI C'CryG_G
Cuts at: 107 107
Size: 3764
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1714 1895 2058 1714
Size: 181 163 3420
Fragments arranged by size:
3420 181 163
BtsI GCAGTG_nn'
Cuts at: 1007 1007
Size: 3764
DraI TTT'AAA
Cuts at: 252 763 252
Size: 511 3253
DrdI GACnn_nn'nnGTC
Cuts at: 1470 2328 1470
Size: 858 2906
EaeI y'GGCC_r
Cuts at: 1349 1523 1914 1941 3286 1349
Size: 174 391 27 1345 1827
Fragments arranged by size:
1827 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1349 1349
Size: 3764
EarI CTCTTCn'nnn_
Cuts at: 155 1787 1997 155
Size: 1632 210 1922
Fragments arranged by size:
1922 1632 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1972 2282 2428 3574 1972
Size: 310 146 1146 2162
Fragments arranged by size:
2162 1146 310 146
EcoICRI GAG'CTC
Cuts at: 776 776
Size: 3764
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1589 2021 2768 3036 1589
Size: 432 747 268 2317
Fragments arranged by size:
2317 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 903 903
Size: 3764
EcoRI G'AATT_C
Cuts at: 768 768
Size: 3764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 287 1783 287
Size: 1496 2268
FspI TGC'GCA
Cuts at: 1545 1545
Size: 3764
HaeII r_GCGC'y
Cuts at: 563 1198 1446 2468 3447 563
Size: 635 248 1022 979 880
Fragments arranged by size:
1022 979 880 635 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 224 256 681 713 1606 1636 1638 1668 224
Size: 32 425 32 893 30 2 30 2320
Fragments arranged by size:
2320 893 425 32 32 30 30 2
HincII GTy'rAC
Cuts at: 803 803
Size: 3764
KasI G'GCGC_C
Cuts at: 1194 1442 1194
Size: 248 3516
KpnI G_GTAC'C
Cuts at: 784 784
Size: 3764
MluI A'CGCG_T
Cuts at: 3197 3197
Size: 3764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2435 2619 2435
Size: 184 3580
MscI TGG'CCA
Cuts at: 1525 1525
Size: 3764
MslI CAynn'nnrTG
Cuts at: 510 1753 1880 510
Size: 1243 127 2394
Fragments arranged by size:
2394 1243 127
MspA1I CmG'CkG
Cuts at: 348 875 1189 1549 2562 2807 348
Size: 527 314 360 1013 245 1305
Fragments arranged by size:
1305 1013 527 360 314 245
NaeI GCC'GGC
Cuts at: 1945 1945
Size: 3764
NarI GG'CG_CC
Cuts at: 1195 1443 1195
Size: 248 3516
NcoI C'CATG_G
Cuts at: 107 107
Size: 3764
NgoMIV G'CCGG_C
Cuts at: 1943 1943
Size: 3764
NspI r_CATG'y
Cuts at: 817 1317 2224 817
Size: 500 907 2357
Fragments arranged by size:
2357 907 500
PciI A'CATG_T
Cuts at: 2220 2220
Size: 3764
PmeI GTTT'AAAC
Cuts at: 763 763
Size: 3764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2903 2935 2903
Size: 32 3732
PstI C_TGCA'G
Cuts at: 811 1496 811
Size: 685 3079
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3764
PvuII CAG'CTG
Cuts at: 348 1189 1549 348
Size: 841 360 2563
Fragments arranged by size:
2563 841 360
RsrII CG'GwC_CG
Cuts at: 1959 1959
Size: 3764
SacI G_AGCT'C
Cuts at: 778 778
Size: 3764
SalI G'TCGA_C
Cuts at: 801 801
Size: 3764
SapI GCTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
SbfI CC_TGCA'GG
Cuts at: 811 811
Size: 3764
ScaI AGT'ACT
Cuts at: 3281 3281
Size: 3764
SfcI C'TryA_G
Cuts at: 33 155 223 540 807 1492 2485 2676 33
Size: 122 68 317 267 685 993 191 1121
Fragments arranged by size:
1121 993 685 317 267 191 122 68
SfoI GGC'GCC
Cuts at: 1196 1444 1196
Size: 248 3516
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
SmaI CCC'GGG
Cuts at: 786 786
Size: 3764
SmlI C'TyrA_G
Cuts at: 919 1834 2326 2588 919
Size: 915 492 262 2095
Fragments arranged by size:
2095 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3764
SphI G_CATG'C
Cuts at: 817 1317 817
Size: 500 3264
StyI C'CwwG_G
Cuts at: 107 898 1252 107
Size: 791 354 2619
Fragments arranged by size:
2619 791 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1347 1480 1939 2148 1347
Size: 133 459 209 2963
Fragments arranged by size:
2963 459 209 133
TatI w'GTAC_w
Cuts at: 332 3279 332
Size: 2947 817
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 123 155 3281 3313 123
Size: 32 3126 32 574
Fragments arranged by size:
3126 574 32 32
Tth111I GACn'n_nGTC
Cuts at: 1561 1561
Size: 3764
XbaI T'CTAG_A
Cuts at: 795 795
Size: 3764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1259 1259
Size: 3764
XmaI C'CCGG_G
Cuts at: 784 784
Size: 3764
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsgI BsiWI BsmI BssHII BstEII
BstXI Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI