Restriction map for
pF1KE2695
(Circular) (Six-base) MAPSORT of: pF1KE2695.seq Check: 1069 from: 1 to: 10130
pF1KE2695 10130 bp
With 182 enzymes: SgfI *
July 6, 2017 13:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 726 2528 4953 726
Size: 1802 2425 5903
Fragments arranged by size:
5903 2425 1802
AccI GT'mk_AC
Cuts at: 2642 3971 4301 7168 2642
Size: 1329 330 2867 5604
Fragments arranged by size:
5604 2867 1329 330
Acc65I G'GTAC_C
Cuts at: 1268 4268 5628 7146 1268
Size: 3000 1360 1518 4252
Fragments arranged by size:
4252 3000 1518 1360
AclI AA'CG_TT
Cuts at: 9970 9970
Size: 10130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 727 1246 1375 1720 2354 3964 4687 5806
Size: 403 519 129 345 634 1610 723 1119
Cuts at: 5806 7955 8387 9134 9402 324
Size: 2149 432 747 268 1052
Fragments arranged by size:
2149 1610 1119 1052 747 723 634 519
432 403 345 268 129
AfeI AGC'GCT
Cuts at: 6166 6484 6166
Size: 318 9812
AflIII A'CryG_T
Cuts at: 4910 5351 5372 6995 7482 8586 9563 4910
Size: 441 21 1623 487 1104 977 5477
Fragments arranged by size:
5477 1623 1104 977 487 441 21
AgeI A'CCGG_T
Cuts at: 1415 8518 1415
Size: 7103 3027
AhdI GACnn_n'nnGTC
Cuts at: 3218 3218
Size: 10130
AleI CACnn'nnGTG
Cuts at: 4564 4564
Size: 10130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 5147 5461 5147
Size: 314 9816
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1501 1533 1501
Size: 32 10098
AlwNI CAG_nnn'CTG
Cuts at: 2085 2445 3245 6849 6897 7435 9002 2085
Size: 360 800 3604 48 538 1567 3213
Fragments arranged by size:
3604 3213 1567 800 538 360 48
ApaI G_GGCC'C
Cuts at: 151 171 202 1721 1845 2594 2804 3005 3051
Size: 20 31 1519 124 749 210 201 46
Cuts at: 3051 3367 6061 6320 151
Size: 316 2694 259 3961
Fragments arranged by size:
3961 2694 1519 749 316 259 210 201
124 46 31 20
ApaLI G'TGCA_C
Cuts at: 8900 8900
Size: 10130
ApoI r'AATT_y
Cuts at: 7134 9483 9739 7134
Size: 2349 256 7525
Fragments arranged by size:
7525 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 10130
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10130
AvaI C'yCGr_G
Cuts at: 170 201 208 1173 2485 2632 2750 2842 2993
Size: 31 7 965 1312 147 118 92 151
Cuts at: 2993 3005 3173 5411 5831 6378 6969 7071 7150
Size: 12 168 2238 420 547 591 102 79
Cuts at: 7150 170
Size: 3150
Fragments arranged by size:
3150 2238 1312 965 591 547 420 168
151 147 118 102 92 79 31 12
7
AvrII C'CTAG_G
Cuts at: 5268 5268
Size: 10130
BamHI G'GATC_C
Cuts at: 638 1441 2931 3820 7155 638
Size: 803 1490 889 3335 3613
Fragments arranged by size:
3613 3335 1490 889 803
BanI G'GyrC_C
Cuts at: 1268 2657 2666 2746 2858 2924 2953 2978 3041
Size: 1389 9 80 112 66 29 25 63
Cuts at: 3041 3793 4268 5628 7146 7560 7808 7843 1268
Size: 752 475 1360 1518 414 248 35 3555
Fragments arranged by size:
3555 1518 1389 1360 752 475 414 248
112 80 66 63 35 29 25 9
BanII G_rGCy'C
Cuts at: 151 171 202 512 586 893 1262 1489 1721
Size: 20 31 310 74 307 369 227 232
Cuts at: 1721 1845 1888 2594 2744 2804 2903 3005 3051
Size: 124 43 706 150 60 99 102 46
Cuts at: 3051 3158 3367 4170 5832 6061 6074 6320 6521
Size: 107 209 803 1662 229 13 246 201
Cuts at: 6521 6622 6756 7144 8174 151
Size: 101 134 388 1030 2107
Fragments arranged by size:
2107 1662 1030 803 706 388 369 310
307 246 232 229 227 209 201 150
134 124 107 102 101 99 74 60
46 43 31 20 13
BbeI G_GCGC'C
Cuts at: 2957 3797 7564 7812 2957
Size: 840 3767 248 5275
Fragments arranged by size:
5275 3767 840 248
BbsI GAAGACnn'nnnn_
Cuts at: 330 564 1483 1965 2067 2542 5617 330
Size: 234 919 482 102 475 3075 4843
Fragments arranged by size:
4843 3075 919 482 475 234 102
BbvCI CC'TCA_GC
Cuts at: 697 1855 3614 4798 5866 6541 6838 697
Size: 1158 1759 1184 1068 675 297 3989
Fragments arranged by size:
3989 1759 1184 1158 1068 675 297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4413 4447 5305 5339 4413
Size: 34 858 34 9204
Fragments arranged by size:
9204 858 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8028 8789 10130 8028
Size: 761 1341 8028
Fragments arranged by size:
8028 1341 761
BclI T'GATC_A
Cuts at: 4349 4832 5225 7650 4349
Size: 483 393 2425 6829
Fragments arranged by size:
6829 2425 483 393
BglI GCCn_nnn'nGGC
Cuts at: 155 190 1632 2579 2725 2846 3036 3802 5388
Size: 35 1442 947 146 121 190 766 1586
Cuts at: 5388 155
Size: 4897
Fragments arranged by size:
4897 1586 1442 947 766 190 146 121
35
BglII A'GATC_T
Cuts at: 1425 7645 9682 1425
Size: 6220 2037 1873
Fragments arranged by size:
6220 2037 1873
BlpI GC'TnA_GC
Cuts at: 4115 6790 7242 4115
Size: 2675 452 7003
Fragments arranged by size:
7003 2675 452
Bme1580I G_kGCm'C
Cuts at: 151 171 202 1721 1845 2594 2751 2804 3005
Size: 20 31 1519 124 749 157 53 201
Cuts at: 3005 3051 3367 5929 6061 6320 7040 7755 7848
Size: 46 316 2562 132 259 720 715 93
Cuts at: 7848 8904 151
Size: 1056 1377
Fragments arranged by size:
2562 1519 1377 1056 749 720 715 316
259 201 157 132 124 93 53 46
31 20
BmgBI CAC'GTC
Cuts at: 1350 3453 4499 5483 1350
Size: 2103 1046 984 5997
Fragments arranged by size:
5997 2103 1046 984
BmrI ACTGGGnnnn_n'
Cuts at: 1572 3343 3386 3616 7008 7342 7423 7758 9902
Size: 1771 43 230 3392 334 81 335 2144
Cuts at: 9902 1572
Size: 1800
Fragments arranged by size:
3392 2144 1800 1771 335 334 230 81
43
BmtI G_CTAG'C
Cuts at: 6647 6647
Size: 10130
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5934 6853 8497 5934
Size: 919 1644 7567
Fragments arranged by size:
7567 1644 919
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 853 1294 1902 3214 3235 3761 4081 4423
Size: 384 441 608 1312 21 526 320 342
Cuts at: 4423 5536 5762 5818 5992 6106 6328 6966 469
Size: 1113 226 56 174 114 222 638 3633
Fragments arranged by size:
3633 1312 1113 638 608 526 441 384
342 320 226 222 174 114 56 21
Bpu10I CC'TnA_GC
Cuts at: 697 1855 3614 4798 5101 5866 6541 6838 697
Size: 1158 1759 1184 303 765 675 297 3989
Fragments arranged by size:
3989 1759 1184 1158 765 675 303 297
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 1450 3612 3868 3895 7306 8185 8677 8975
Size: 720 2162 256 27 3411 879 492 298
Cuts at: 8975 730
Size: 1885
Fragments arranged by size:
3411 2162 1885 879 720 492 298 256
27
BsaI GGTCTCn'nnnn_
Cuts at: 1681 3085 3190 4518 5714 6824 9834 1681
Size: 1404 105 1328 1196 1110 3010 1977
Fragments arranged by size:
3010 1977 1404 1328 1196 1110 105
BsaAI yAC'GTr
Cuts at: 4296 4911 6996 7483 4296
Size: 615 2085 487 6943
Fragments arranged by size:
6943 2085 615 487
BsaBI GATnn'nnATC
Cuts at: 6 550 4354 7644 9681 6
Size: 544 3804 3290 2037 455
Fragments arranged by size:
3804 3290 2037 544 455
BsaHI Gr'CG_yC
Cuts at: 2141 2639 2907 2954 3794 6247 6685 7561 7809
Size: 498 268 47 840 2453 438 876 248
Cuts at: 7809 8511 10007 2141
Size: 702 1496 2264
Fragments arranged by size:
2453 2264 1496 876 840 702 498 438
268 248 47
BsaWI w'CCGG_w
Cuts at: 1415 1499 1520 1733 3158 5021 5504 7319 7840
Size: 84 21 213 1425 1863 483 1815 521
Cuts at: 7840 8518 8792 8939 9350 1415
Size: 678 274 147 411 2195
Fragments arranged by size:
2195 1863 1815 1425 678 521 483 411
274 213 147 84 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1501 1531 1870 1900 5158 5188 5819 5849 6985
Size: 30 339 30 3258 30 631 30 1136
Cuts at: 6985 7015 1501
Size: 30 4616
Fragments arranged by size:
4616 3258 1136 631 339 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1327 1345 1417 2290 2503 2770 2896 3040 3474
Size: 18 72 873 213 267 126 144 434
Cuts at: 3474 4261 5840 6456 6459 6720 6964 6969 1327
Size: 787 1579 616 3 261 244 5 4488
Fragments arranged by size:
4488 1579 873 787 616 434 267 261
244 213 144 126 72 18 5 3
BseYI C'CCAG_C
Cuts at: 129 924 1161 1983 2568 3238 5391 5532 5536
Size: 795 237 822 585 670 2153 141 4
Cuts at: 5536 6074 6185 6319 6703 7555 8313 8890 9386
Size: 538 111 134 384 852 758 577 496
Cuts at: 9386 129
Size: 873
Fragments arranged by size:
2153 873 852 822 795 758 670 585
577 538 496 384 237 141 134 111
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 4023 4030 7038 4023
Size: 7 3008 7115
Fragments arranged by size:
7115 3008 7
BsiEI CG_ry'CG
Cuts at: 104 138 254 1088 2554 2764 2770 2829 2853
Size: 34 116 834 1466 210 6 59 24
Cuts at: 2853 2891 3386 6128 6501 6657 7672 7718 8926
Size: 38 495 2742 373 156 1015 46 1208
Cuts at: 8926 104
Size: 1308
Fragments arranged by size:
2742 1466 1308 1208 1015 834 495 373
210 156 116 59 46 38 34 24
6
BsiHKAI G_wGCw'C
Cuts at: 746 1172 1262 1489 1888 3158 4163 5194 5822
Size: 426 90 227 399 1270 1005 1031 628
Cuts at: 5822 6018 7086 7144 7922 8112 8904 746
Size: 196 1068 58 778 190 792 1972
Fragments arranged by size:
1972 1270 1068 1031 1005 792 778 628
426 399 227 196 190 90 58
BsmI GAATG_Cn'
Cuts at: 1329 2109 4399 4663 1329
Size: 780 2290 264 6796
Fragments arranged by size:
6796 2290 780 264
BsmBI CGTCTCn'nnnn_
Cuts at: 5178 5178
Size: 10130
Bsp1286I G_dGCh'C
Cuts at: 151 171 202 512 586 746 893 1172 1262
Size: 20 31 310 74 160 147 279 90
Cuts at: 1262 1489 1721 1845 1888 2594 2744 2751 2804
Size: 227 232 124 43 706 150 7 53
Cuts at: 2804 2903 3005 3051 3158 3367 4163 4170 5194
Size: 99 102 46 107 209 796 7 1024
Cuts at: 5194 5822 5832 5929 6018 6061 6074 6320 6521
Size: 628 10 97 89 43 13 246 201
Cuts at: 6521 6622 6756 7040 7086 7144 7755 7848 7922
Size: 101 134 284 46 58 611 93 74
Cuts at: 7922 8112 8174 8904 151
Size: 190 62 730 1377
Fragments arranged by size:
1377 1024 796 730 706 628 611 310
284 279 246 232 227 209 201 190
160 150 147 134 124 107 102 101
99 97 93 90 89 74 74 62
58 53 46 46 43 43 31 20
13 10 7 7
BspEI T'CCGG_A
Cuts at: 1499 3158 5021 5504 9350 1499
Size: 1659 1863 483 3846 2279
Fragments arranged by size:
3846 2279 1863 1659 483
BspHI T'CATG_A
Cuts at: 755 9306 755
Size: 8551 1579
BspMI ACCTGCnnnn'nnnn_
Cuts at: 599 677 726 2453 2528 3527 4953 7180 7394
Size: 78 49 1727 75 999 1426 2227 214
Cuts at: 7394 7696 8077 599
Size: 302 381 2652
Fragments arranged by size:
2652 2227 1727 1426 999 381 302 214
78 75 49
BsrBI CCG'CTC
Cuts at: 50 165 326 492 1363 1472 2493 2735 2760
Size: 115 161 166 871 109 1021 242 25
Cuts at: 2760 2895 2967 2998 3020 3464 6103 6429 6447
Size: 135 72 31 22 444 2639 326 18
Cuts at: 6447 6977 8422 8476 9960 10125 50
Size: 530 1445 54 1484 165 55
Fragments arranged by size:
2639 1484 1445 1021 871 530 444 326
242 166 165 161 135 115 109 72
55 54 31 25 22 18
BsrDI GCAATG_nn'
Cuts at: 4001 4922 5877 8042 4001
Size: 921 955 2165 6089
Fragments arranged by size:
6089 2165 955 921
BsrFI r'CCGG_y
Cuts at: 184 1087 1235 1415 2549 2663 2852 2864 2921
Size: 903 148 180 1134 114 189 12 57
Cuts at: 2921 2950 3030 4475 5811 6401 6419 6434 6785
Size: 29 80 1445 1336 590 18 15 351
Cuts at: 6785 8128 8309 8518 184
Size: 1343 181 209 1796
Fragments arranged by size:
1796 1445 1343 1336 1134 903 590 351
209 189 181 180 148 114 80 57
29 18 15 12
BsrGI T'GTAC_A
Cuts at: 281 2468 3339 281
Size: 2187 871 7072
Fragments arranged by size:
7072 2187 871
BssHII G'CGCG_C
Cuts at: 261 263 2398 2582 2915 2970 2972 261
Size: 2 2135 184 333 55 2 7419
Fragments arranged by size:
7419 2135 333 184 55 2 2
BssSI C'ACGA_G
Cuts at: 3073 3577 5080 8401 8759 3073
Size: 504 1503 3321 358 4444
Fragments arranged by size:
4444 3321 1503 504 358
BstAPI GCAn_nnn'nTGC
Cuts at: 741 2445 2945 3234 7507 741
Size: 1704 500 289 4273 3364
Fragments arranged by size:
4273 3364 1704 500 289
BstBI TT'CG_AA
Cuts at: 8491 8491
Size: 10130
BstEII G'GTnAC_C
Cuts at: 7045 7045
Size: 10130
BstXI CCAn_nnnn'nTGG
Cuts at: 2232 2328 4059 5493 5899 2232
Size: 96 1731 1434 406 6463
Fragments arranged by size:
6463 1731 1434 406 96
BstYI r'GATC_y
Cuts at: 1 524 638 1280 1425 1441 1859 2081 2931
Size: 523 114 642 145 16 418 222 850
Cuts at: 2931 3820 5993 7155 7622 7645 7980 8226 9238
Size: 889 2173 1162 467 23 335 246 1012
Cuts at: 9238 9324 9336 9682 1
Size: 86 12 346 449
Fragments arranged by size:
2173 1162 1012 889 850 642 523 467
449 418 346 335 246 222 145 114
86 23 16 12
Bsu36I CC'TnA_GG
Cuts at: 2615 6929 2615
Size: 4314 5816
BtgI C'CryG_G
Cuts at: 107 272 647 764 893 1043 1607 1627 2594
Size: 165 375 117 129 150 564 20 967
Cuts at: 2594 2690 2764 2795 2982 3095 3110 3380 4193
Size: 96 74 31 187 113 15 270 813
Cuts at: 4193 4211 4229 4652 5063 6195 6657 6795 107
Size: 18 18 423 411 1132 462 138 3442
Fragments arranged by size:
3442 1132 967 813 564 462 423 411
375 270 187 165 150 138 129 117
113 96 74 31 20 18 18 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 408 540 2211 2352 3570 3585 4659 8080 8261
Size: 132 1671 141 1218 15 1074 3421 181
Cuts at: 8261 8424 408
Size: 163 2114
Fragments arranged by size:
3421 2114 1671 1218 1074 181 163 141
132 15
BtsI GCAGTG_nn'
Cuts at: 2114 3232 4132 6360 6606 7050 7373 2114
Size: 1118 900 2228 246 444 323 4871
Fragments arranged by size:
4871 2228 1118 900 444 323 246
ClaI AT'CG_AT
Cuts at: 250 250
Size: 10130
DraI TTT'AAA
Cuts at: 7129 7129
Size: 10130
DraIII CAC_nnn'GTG
Cuts at: 386 953 1598 1600 3187 4230 5141 386
Size: 567 645 2 1587 1043 911 5375
Fragments arranged by size:
5375 1587 1043 911 645 567 2
DrdI GACnn_nn'nnGTC
Cuts at: 355 622 1762 7014 7836 8694 355
Size: 267 1140 5252 822 858 1791
Fragments arranged by size:
5252 1791 1140 858 822 267
EaeI y'GGCC_r
Cuts at: 135 225 413 967 1085 1307 1364 1604 1799
Size: 90 188 554 118 222 57 240 195
Cuts at: 1799 2222 2243 2390 2551 2761 2767 2826 2850
Size: 423 21 147 161 210 6 59 24
Cuts at: 2850 2888 4541 5979 6125 6570 6654 6722 6959
Size: 38 1653 1438 146 445 84 68 237
Cuts at: 6959 7077 7715 7889 8280 8307 9652 135
Size: 118 638 174 391 27 1345 613
Fragments arranged by size:
1653 1438 1345 638 613 554 445 423
391 240 237 222 210 195 188 174
161 147 146 118 118 90 84 68
59 57 38 27 24 21 6
EagI C'GGCC_G
Cuts at: 135 1085 2551 2761 2767 2826 2850 2888 6125
Size: 950 1466 210 6 59 24 38 3237
Cuts at: 6125 6654 7715 135
Size: 529 1061 2550
Fragments arranged by size:
3237 2550 1466 1061 950 529 210 59
38 24 6
EarI CTCTTCn'nnn_
Cuts at: 337 802 1351 1369 1684 1951 1960 2267 3985
Size: 465 549 18 315 267 9 307 1718
Cuts at: 3985 4021 5265 5845 8153 8363 337
Size: 36 1244 580 2308 210 2104
Fragments arranged by size:
2308 2104 1718 1244 580 549 465 315
307 267 210 36 18 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 173 1306 1837 2743 3028 3514 4798 4834 6681
Size: 1133 531 906 285 486 1284 36 1847
Cuts at: 6681 7018 8338 8648 8794 9940 173
Size: 337 1320 310 146 1146 363
Fragments arranged by size:
1847 1320 1284 1146 1133 906 531 486
363 337 310 285 146 36
EcoICRI GAG'CTC
Cuts at: 1260 1487 1886 3156 7142 1260
Size: 227 399 1270 3986 4248
Fragments arranged by size:
4248 3986 1270 399 227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 469 727 853 1246 1294 1375 1720 1902
Size: 145 258 126 393 48 81 345 182
Cuts at: 1902 2354 3214 3235 3761 3964 4081 4423 4687
Size: 452 860 21 526 203 117 342 264
Cuts at: 4687 5536 5762 5806 5818 5992 6106 6328 6966
Size: 849 226 44 12 174 114 222 638
Cuts at: 6966 7955 8387 9134 9402 324
Size: 989 432 747 268 1052
Fragments arranged by size:
1052 989 860 849 747 638 526 452
432 393 345 342 268 264 258 226
222 203 182 174 145 126 117 114
81 48 44 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 6310 6928 6310
Size: 618 9512
EcoO109I rG'GnC_Cy
Cuts at: 147 148 198 205 234 701 921 973 1717
Size: 1 50 7 29 467 220 52 744
Cuts at: 1717 1842 2590 2628 2718 2800 2801 2839 3001
Size: 125 748 38 90 82 1 38 162
Cuts at: 3001 3002 3220 3364 3618 4899 5272 5761 5936
Size: 1 218 144 254 1281 373 489 175
Cuts at: 5936 6057 6058 6302 6316 6388 6583 7269 147
Size: 121 1 244 14 72 195 686 3008
Fragments arranged by size:
3008 1281 748 744 686 489 467 373
254 244 220 218 195 175 162 144
125 121 90 82 72 52 50 38
38 29 14 7 1 1 1 1
EcoRI G'AATT_C
Cuts at: 7134 7134
Size: 10130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6501 8149 6501
Size: 1648 8482
FspI TGC'GCA
Cuts at: 3628 7911 3628
Size: 4283 5847
FspAI rTGC'GCAy
Cuts at: 3628 3628
Size: 10130
HaeII r_GCGC'y
Cuts at: 280 2467 2957 3797 4445 5063 5430 6168 6420
Size: 2187 490 840 648 618 367 738 252
Cuts at: 6420 6486 7564 7812 8834 9813 280
Size: 66 1078 248 1022 979 597
Fragments arranged by size:
2187 1078 1022 979 840 738 648 618
597 490 367 252 248 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1321 1353 1474 1506 3204 3236 3351 3383 6081
Size: 32 121 32 1698 32 115 32 2698
Cuts at: 6081 6113 7972 8002 8004 8034 1321
Size: 32 1859 30 2 30 3417
Fragments arranged by size:
3417 2698 1859 1698 121 115 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 321 2643 4332 6632 7169 321
Size: 2322 1689 2300 537 3282
Fragments arranged by size:
3282 2322 2300 1689 537
HindIII A'AGCT_T
Cuts at: 2302 2302
Size: 10130
KasI G'GCGC_C
Cuts at: 2953 3793 7560 7808 2953
Size: 840 3767 248 5275
Fragments arranged by size:
5275 3767 840 248
KpnI G_GTAC'C
Cuts at: 1272 4272 5632 7150 1272
Size: 3000 1360 1518 4252
Fragments arranged by size:
4252 3000 1518 1360
MluI A'CGCG_T
Cuts at: 9563 9563
Size: 10130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1419 5480 8801 8985 1419
Size: 4061 3321 184 2564
Fragments arranged by size:
4061 3321 2564 184
MscI TGG'CCA
Cuts at: 415 969 1606 2224 2245 4543 5981 6572 6961
Size: 554 637 618 21 2298 1438 591 389
Cuts at: 6961 7891 415
Size: 930 2654
Fragments arranged by size:
2654 2298 1438 930 637 618 591 554
389 21
MslI CAynn'nnrTG
Cuts at: 811 1042 1690 2230 3304 3577 4006 4339 4543
Size: 231 648 540 1074 273 429 333 204
Cuts at: 4543 4564 4642 4927 4957 4963 5285 5443 5491
Size: 21 78 285 30 6 322 158 48
Cuts at: 5491 5555 8119 8246 811
Size: 64 2564 127 2695
Fragments arranged by size:
2695 2564 1074 648 540 429 333 322
285 273 231 204 158 127 78 64
48 30 21 6
MspA1I CmG'CkG
Cuts at: 231 1629 1995 2418 2456 2556 2692 2766 2797
Size: 1398 366 423 38 100 136 74 31
Cuts at: 2797 2984 3291 5536 6189 6272 6568 6659 7241
Size: 187 307 2245 653 83 296 91 582
Cuts at: 7241 7555 7915 8928 9173 231
Size: 314 360 1013 245 1188
Fragments arranged by size:
2245 1398 1188 1013 653 582 423 366
360 314 307 296 245 187 136 100
91 83 74 38 31
NaeI GCC'GGC
Cuts at: 186 1089 1237 2854 2952 3032 5813 6403 6421
Size: 903 148 1617 98 80 2781 590 18
Cuts at: 6421 6787 8311 186
Size: 366 1524 2005
Fragments arranged by size:
2781 2005 1617 1524 903 590 366 148
98 80 18
NarI GG'CG_CC
Cuts at: 2954 3794 7561 7809 2954
Size: 840 3767 248 5275
Fragments arranged by size:
5275 3767 840 248
NcoI C'CATG_G
Cuts at: 107 272 764 1043 1607 4211 4652 107
Size: 165 492 279 564 2604 441 5585
Fragments arranged by size:
5585 2604 564 492 441 279 165
NgoMIV G'CCGG_C
Cuts at: 184 1087 1235 2852 2950 3030 5811 6401 6419
Size: 903 148 1617 98 80 2781 590 18
Cuts at: 6419 6785 8309 184
Size: 366 1524 2005
Fragments arranged by size:
2781 2005 1617 1524 903 590 366 148
98 80 18
NheI G'CTAG_C
Cuts at: 6643 6643
Size: 10130
NotI GC'GGCC_GC
Cuts at: 135 2761 2767 135
Size: 2626 6 7498
Fragments arranged by size:
7498 2626 6
NspI r_CATG'y
Cuts at: 2157 3978 4467 4962 5355 5376 5448 7183 7683
Size: 1821 489 495 393 21 72 1735 500
Cuts at: 7683 8590 2157
Size: 907 3697
Fragments arranged by size:
3697 1821 1735 907 500 495 489 393
72 21
PasI CC'CwG_GG
Cuts at: 4264 7059 4264
Size: 2795 7335
PciI A'CATG_T
Cuts at: 5351 5372 8586 5351
Size: 21 3214 6895
Fragments arranged by size:
6895 3214 21
PflMI CCAn_nnn'nTGG
Cuts at: 3258 4127 4264 6568 6849 3258
Size: 869 137 2304 281 6539
Fragments arranged by size:
6539 2304 869 281 137
PfoI T'CCnGG_A
Cuts at: 3752 4364 5739 6982 3752
Size: 612 1375 1243 6900
Fragments arranged by size:
6900 1375 1243 612
PmeI GTTT'AAAC
Cuts at: 7129 7129
Size: 10130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1501 1533 9269 9301 1501
Size: 32 7736 32 2330
Fragments arranged by size:
7736 2330 32 32
PpuMI rG'GwC_Cy
Cuts at: 205 234 973 3220 3618 5272 5761 5936 6302
Size: 29 739 2247 398 1654 489 175 366
Cuts at: 6302 6583 205
Size: 281 3752
Fragments arranged by size:
3752 2247 1654 739 489 398 366 281
175 29
PshAI GACnn'nnGTC
Cuts at: 4882 4882
Size: 10130
PspOMI G'GGCC_C
Cuts at: 147 167 198 1717 1841 2590 2800 3001 3047
Size: 20 31 1519 124 749 210 201 46
Cuts at: 3047 3363 6057 6316 147
Size: 316 2694 259 3961
Fragments arranged by size:
3961 2694 1519 749 316 259 210 201
124 46 31 20
PspXI vC'TCGA_Gb
Cuts at: 5411 5411
Size: 10130
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1093 1125 1093
Size: 32 10098
PstI C_TGCA'G
Cuts at: 221 782 2441 4075 6827 7177 7862 221
Size: 561 1659 1634 2752 350 685 2489
Fragments arranged by size:
2752 2489 1659 1634 685 561 350
PvuI CG_AT'CG
Cuts at: 104 254 104
Size: 150 9980
PvuII CAG'CTG
Cuts at: 1995 2456 5536 6568 7555 7915 1995
Size: 461 3080 1032 987 360 4210
Fragments arranged by size:
4210 3080 1032 987 461 360
RsrII CG'GwC_CG
Cuts at: 6225 8325 6225
Size: 2100 8030
SacI G_AGCT'C
Cuts at: 1262 1489 1888 3158 7144 1262
Size: 227 399 1270 3986 4248
Fragments arranged by size:
4248 3986 1270 399 227
SacII CC_GC'GG
Cuts at: 1630 2693 2767 2798 2985 6660 1630
Size: 1063 74 31 187 3675 5100
Fragments arranged by size:
5100 3675 1063 187 74 31
SalI G'TCGA_C
Cuts at: 2641 7167 2641
Size: 4526 5604
SanDI GG'GwC_CC
Cuts at: 205 3220 6583 205
Size: 3015 3363 3752
Fragments arranged by size:
3752 3363 3015
SapI GCTCTTCn'nnn_
Cuts at: 337 1369 1951 1960 3985 4021 8153 8363 337
Size: 1032 582 9 2025 36 4132 210 2104
Fragments arranged by size:
4132 2104 2025 1032 582 210 36 9
SbfI CC_TGCA'GG
Cuts at: 7177 7177
Size: 10130
ScaI AGT'ACT
Cuts at: 964 1879 9647 964
Size: 915 7768 1447
Fragments arranged by size:
7768 1447 915
SexAI A'CCwGG_T
Cuts at: 1154 1898 1154
Size: 744 9386
SfcI C'TryA_G
Cuts at: 33 217 778 2437 4071 6392 6823 7173 7858
Size: 184 561 1659 1634 2321 431 350 685
Cuts at: 7858 8851 9042 33
Size: 993 191 1121
Fragments arranged by size:
2321 1659 1634 1121 993 685 561 431
350 191 184
SfoI GGC'GCC
Cuts at: 2955 3795 7562 7810 2955
Size: 840 3767 248 5275
Fragments arranged by size:
5275 3767 840 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10130
SgrAI Cr'CCGG_yG
Cuts at: 2864 2950 2864
Size: 86 10044
SmaI CCC'GGG
Cuts at: 172 203 210 2752 3007 3175 5833 6380 7152
Size: 31 7 2542 255 168 2658 547 772
Cuts at: 7152 172
Size: 3150
Fragments arranged by size:
3150 2658 2542 772 547 255 168 31
7
SmlI C'TyrA_G
Cuts at: 745 1465 3591 3883 3910 5411 7285 8200 8692
Size: 720 2126 292 27 1501 1874 915 492
Cuts at: 8692 8954 745
Size: 262 1921
Fragments arranged by size:
2126 1921 1874 1501 915 720 492 292
262 27
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10130
SphI G_CATG'C
Cuts at: 4962 7183 7683 4962
Size: 2221 500 7409
Fragments arranged by size:
7409 2221 500
SspI AAT'ATT
Cuts at: 4977 4977
Size: 10130
StuI AGG'CCT
Cuts at: 862 5074 5113 5747 6910 862
Size: 4212 39 634 1163 4082
Fragments arranged by size:
4212 4082 1163 634 39
StyI C'CwwG_G
Cuts at: 107 272 764 1043 1187 1607 2264 2393 2957
Size: 165 492 279 144 420 657 129 564
Cuts at: 2957 4052 4211 4652 5268 7264 7618 107
Size: 1095 159 441 616 1996 354 2619
Fragments arranged by size:
2619 1996 1095 657 616 564 492 441
420 354 279 165 159 144 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 886 1554 2723 6566 6605 6750 7713 7846 8305
Size: 668 1169 3843 39 145 963 133 459
Cuts at: 8305 8514 886
Size: 209 2502
Fragments arranged by size:
3843 2502 1169 963 668 459 209 145
133 39
TatI w'GTAC_w
Cuts at: 281 962 1877 2468 3339 9645 281
Size: 681 915 591 871 6306 766
Fragments arranged by size:
6306 915 871 766 681 591
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3372 3404 5467 5499 9647 9679 3372
Size: 32 2063 32 4148 32 3823
Fragments arranged by size:
4148 3823 2063 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 352 4726 7927 352
Size: 4374 3201 2555
Fragments arranged by size:
4374 3201 2555
XbaI T'CTAG_A
Cuts at: 7161 7161
Size: 10130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 828 3399 4293 4650 5967 6177 6193 7625 828
Size: 2571 894 357 1317 210 16 1432 3333
Fragments arranged by size:
3333 2571 1432 1317 894 357 210 16
XhoI C'TCGA_G
Cuts at: 5411 5411
Size: 10130
XmaI C'CCGG_G
Cuts at: 170 201 208 2750 3005 3173 5831 6378 7150
Size: 31 7 2542 255 168 2658 547 772
Cuts at: 7150 170
Size: 3150
Fragments arranged by size:
3150 2658 2542 772 547 255 168 31
7
XmnI GAAnn'nnTTC
Cuts at: 310 1516 4030 4696 4786 310
Size: 1206 2514 666 90 5654
Fragments arranged by size:
5654 2514 1206 666 90
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AscI BaeI BfrBI BsiWI BstZ17I CspCI
EcoRV FseI HpaI MfeI NdeI NruI NsiI PacI
PmlI PsiI SfiI SnaBI SrfI SwaI ZraI