(Circular) (Six-base) MAPSORT of: pF1KE2712.seq Check: 2100 from: 1 to: 3653 pF1KE2712 3653 bp With 182 enzymes: SgfI * January 5, 2018 10:00 .. AccI GT'mk_AC Cuts at: 691 691 Size: 3653 Acc65I G'GTAC_C Cuts at: 669 669 Size: 3653 AclI AA'CG_TT Cuts at: 3493 3493 Size: 3653 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 299 496 1478 1910 2657 2925 299 Size: 197 982 432 747 268 1027 Fragments arranged by size: 1027 982 747 432 268 197 AflIII A'CryG_T Cuts at: 1005 2109 3086 1005 Size: 1104 977 1572 Fragments arranged by size: 1572 1104 977 AgeI A'CCGG_T Cuts at: 2041 2041 Size: 3653 AlwNI CAG_nnn'CTG Cuts at: 560 958 2525 560 Size: 398 1567 1688 Fragments arranged by size: 1688 1567 398 ApaLI G'TGCA_C Cuts at: 2423 2423 Size: 3653 ApoI r'AATT_y Cuts at: 657 3006 3262 657 Size: 2349 256 1048 Fragments arranged by size: 2349 1048 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3653 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3653 AvaI C'yCGr_G Cuts at: 242 267 673 242 Size: 25 406 3222 Fragments arranged by size: 3222 406 25 BamHI G'GATC_C Cuts at: 678 678 Size: 3653 BanI G'GyrC_C Cuts at: 220 283 669 1083 1331 1366 220 Size: 63 386 414 248 35 2507 Fragments arranged by size: 2507 414 386 248 63 35 BanII G_rGCy'C Cuts at: 667 1697 667 Size: 1030 2623 BbeI G_GCGC'C Cuts at: 287 1087 1335 287 Size: 800 248 2605 Fragments arranged by size: 2605 800 248 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 248 282 90 Size: 34 124 34 3461 Fragments arranged by size: 3461 124 34 34 BciVI GTATCCnnnnn_n' Cuts at: 1551 2312 3653 1551 Size: 761 1341 1551 Fragments arranged by size: 1551 1341 761 BclI T'GATC_A Cuts at: 1173 1173 Size: 3653 BglI GCCn_nnn'nGGC Cuts at: 112 164 191 112 Size: 52 27 3574 Fragments arranged by size: 3574 52 27 BglII A'GATC_T Cuts at: 1168 3205 1168 Size: 2037 1616 BlpI GC'TnA_GC Cuts at: 623 765 623 Size: 142 3511 Bme1580I G_kGCm'C Cuts at: 130 1278 1371 2427 130 Size: 1148 93 1056 1356 Fragments arranged by size: 1356 1148 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 865 946 1281 3425 865 Size: 81 335 2144 1093 Fragments arranged by size: 2144 1093 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2020 2020 Size: 3653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 550 550 Size: 3653 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 391 829 1708 2200 2498 391 Size: 438 879 492 298 1546 Fragments arranged by size: 1546 879 492 438 298 BsaI GGTCTCn'nnnn_ Cuts at: 3357 3357 Size: 3653 BsaAI yAC'GTr Cuts at: 1006 1006 Size: 3653 BsaBI GATnn'nnATC Cuts at: 6 589 1167 3204 6 Size: 583 578 2037 455 Fragments arranged by size: 2037 583 578 455 BsaHI Gr'CG_yC Cuts at: 284 1084 1332 2034 3530 284 Size: 800 248 702 1496 407 Fragments arranged by size: 1496 800 702 407 248 BsaWI w'CCGG_w Cuts at: 842 1363 2041 2315 2462 2873 842 Size: 521 678 274 147 411 1622 Fragments arranged by size: 1622 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 293 293 Size: 3653 BseYI C'CCAG_C Cuts at: 1078 1836 2413 2909 1078 Size: 758 577 496 1822 Fragments arranged by size: 1822 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1195 1241 2449 104 Size: 1091 46 1208 1308 Fragments arranged by size: 1308 1208 1091 46 BsiHKAI G_wGCw'C Cuts at: 667 1445 1635 2427 667 Size: 778 190 792 1893 Fragments arranged by size: 1893 792 778 190 BsmI GAATG_Cn' Cuts at: 173 173 Size: 3653 Bsp1286I G_dGCh'C Cuts at: 130 667 1278 1371 1445 1635 1697 2427 130 Size: 537 611 93 74 190 62 730 1356 Fragments arranged by size: 1356 730 611 537 190 93 74 62 BspEI T'CCGG_A Cuts at: 2873 2873 Size: 3653 BspHI T'CATG_A Cuts at: 2829 2829 Size: 3653 BspMI ACCTGCnnnn'nnnn_ Cuts at: 703 917 1219 1600 703 Size: 214 302 381 2756 Fragments arranged by size: 2756 381 302 214 BsrBI CCG'CTC Cuts at: 50 1945 1999 3483 3648 50 Size: 1895 54 1484 165 55 Fragments arranged by size: 1895 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 201 1565 201 Size: 1364 2289 BsrFI r'CCGG_y Cuts at: 1651 1832 2041 1651 Size: 181 209 3263 Fragments arranged by size: 3263 209 181 BssSI C'ACGA_G Cuts at: 1924 2282 1924 Size: 358 3295 BstAPI GCAn_nnn'nTGC Cuts at: 1030 1030 Size: 3653 BstBI TT'CG_AA Cuts at: 2014 2014 Size: 3653 BstYI r'GATC_y Cuts at: 1 678 1145 1168 1503 1749 2761 2847 2859 Size: 677 467 23 335 246 1012 86 12 Cuts at: 2859 3205 1 Size: 346 449 Fragments arranged by size: 1012 677 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 633 633 Size: 3653 BtgI C'CryG_G Cuts at: 224 289 404 224 Size: 65 115 3473 Fragments arranged by size: 3473 115 65 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 161 1603 1784 1947 161 Size: 1442 181 163 1867 Fragments arranged by size: 1867 1442 181 163 BtsI GCAGTG_nn' Cuts at: 604 896 604 Size: 292 3361 DraI TTT'AAA Cuts at: 652 652 Size: 3653 DraIII CAC_nnn'GTG Cuts at: 599 599 Size: 3653 DrdI GACnn_nn'nnGTC Cuts at: 1359 2217 1359 Size: 858 2795 EaeI y'GGCC_r Cuts at: 1238 1412 1803 1830 3175 1238 Size: 174 391 27 1345 1716 Fragments arranged by size: 1716 1345 391 174 27 EagI C'GGCC_G Cuts at: 1238 1238 Size: 3653 EarI CTCTTCn'nnn_ Cuts at: 1676 1886 1676 Size: 210 3443 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1861 2171 2317 3463 1861 Size: 310 146 1146 2051 Fragments arranged by size: 2051 1146 310 146 EcoICRI GAG'CTC Cuts at: 665 665 Size: 3653 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 299 496 550 1478 1910 2657 2925 299 Size: 197 54 928 432 747 268 1027 Fragments arranged by size: 1027 928 747 432 268 197 54 EcoNI CCTnn'n_nnAGG Cuts at: 436 632 436 Size: 196 3457 EcoO109I rG'GnC_Cy Cuts at: 166 238 263 792 166 Size: 72 25 529 3027 Fragments arranged by size: 3027 529 72 25 EcoRI G'AATT_C Cuts at: 657 657 Size: 3653 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1672 1672 Size: 3653 FspI TGC'GCA Cuts at: 1434 1434 Size: 3653 HaeII r_GCGC'y Cuts at: 287 1087 1335 2357 3336 287 Size: 800 248 1022 979 604 Fragments arranged by size: 1022 979 800 604 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1495 1525 1527 1557 1495 Size: 30 2 30 3591 Fragments arranged by size: 3591 30 30 2 HincII GTy'rAC Cuts at: 692 692 Size: 3653 KasI G'GCGC_C Cuts at: 283 1083 1331 283 Size: 800 248 2605 Fragments arranged by size: 2605 800 248 KpnI G_GTAC'C Cuts at: 673 673 Size: 3653 MluI A'CGCG_T Cuts at: 3086 3086 Size: 3653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 537 2324 2508 537 Size: 1787 184 1682 Fragments arranged by size: 1787 1682 184 MscI TGG'CCA Cuts at: 1414 1414 Size: 3653 MslI CAynn'nnrTG Cuts at: 1642 1769 1642 Size: 127 3526 MspA1I CmG'CkG Cuts at: 291 764 1078 1438 2451 2696 291 Size: 473 314 360 1013 245 1248 Fragments arranged by size: 1248 1013 473 360 314 245 NaeI GCC'GGC Cuts at: 1834 1834 Size: 3653 NarI GG'CG_CC Cuts at: 284 1084 1332 284 Size: 800 248 2605 Fragments arranged by size: 2605 800 248 NcoI C'CATG_G Cuts at: 404 404 Size: 3653 NgoMIV G'CCGG_C Cuts at: 1832 1832 Size: 3653 NruI TCG'CGA Cuts at: 397 397 Size: 3653 NspI r_CATG'y Cuts at: 706 1206 2113 706 Size: 500 907 2246 Fragments arranged by size: 2246 907 500 PasI CC'CwG_GG Cuts at: 187 187 Size: 3653 PciI A'CATG_T Cuts at: 2109 2109 Size: 3653 PmeI GTTT'AAAC Cuts at: 652 652 Size: 3653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2792 2824 2792 Size: 32 3621 PpuMI rG'GwC_Cy Cuts at: 238 238 Size: 3653 PstI C_TGCA'G Cuts at: 337 528 545 700 1385 337 Size: 191 17 155 685 2605 Fragments arranged by size: 2605 685 191 155 17 PvuI CG_AT'CG Cuts at: 104 104 Size: 3653 PvuII CAG'CTG Cuts at: 1078 1438 1078 Size: 360 3293 RsrII CG'GwC_CG Cuts at: 1848 1848 Size: 3653 SacI G_AGCT'C Cuts at: 667 667 Size: 3653 SacII CC_GC'GG Cuts at: 292 292 Size: 3653 SalI G'TCGA_C Cuts at: 690 690 Size: 3653 SapI GCTCTTCn'nnn_ Cuts at: 1676 1886 1676 Size: 210 3443 SbfI CC_TGCA'GG Cuts at: 700 700 Size: 3653 ScaI AGT'ACT Cuts at: 3170 3170 Size: 3653 SfcI C'TryA_G Cuts at: 33 333 524 541 696 1381 2374 2565 33 Size: 300 191 17 155 685 993 191 1121 Fragments arranged by size: 1121 993 685 300 191 191 155 17 SfiI GGCCn_nnn'nGGCC Cuts at: 164 164 Size: 3653 SfoI GGC'GCC Cuts at: 285 1085 1333 285 Size: 800 248 2605 Fragments arranged by size: 2605 800 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3653 SmaI CCC'GGG Cuts at: 244 675 244 Size: 431 3222 SmlI C'TyrA_G Cuts at: 370 808 1723 2215 2477 370 Size: 438 915 492 262 1546 Fragments arranged by size: 1546 915 492 438 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3653 SphI G_CATG'C Cuts at: 706 1206 706 Size: 500 3153 StyI C'CwwG_G Cuts at: 404 787 1141 404 Size: 383 354 2916 Fragments arranged by size: 2916 383 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 417 1236 1369 1828 2037 417 Size: 819 133 459 209 2033 Fragments arranged by size: 2033 819 459 209 133 TatI w'GTAC_w Cuts at: 3168 3168 Size: 3653 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3170 3202 3170 Size: 32 3621 Tth111I GACn'n_nGTC Cuts at: 1450 1450 Size: 3653 XbaI T'CTAG_A Cuts at: 684 684 Size: 3653 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1148 1148 Size: 3653 XmaI C'CCGG_G Cuts at: 242 673 242 Size: 431 3222 XmnI GAAnn'nnTTC Cuts at: 481 481 Size: 3653 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NsiI PacI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI