Restriction map for
pF1KE2728
(Circular) (Six-base) MAPSORT of: pF1KE2728.seq Check: 1616 from: 1 to: 5042
pF1KE2728 5042 bp
With 182 enzymes: SgfI *
July 3, 2018 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 312 891 312
Size: 579 4463
AccI GT'mk_AC
Cuts at: 2080 2080
Size: 5042
Acc65I G'GTAC_C
Cuts at: 2058 2058
Size: 5042
AclI AA'CG_TT
Cuts at: 4882 4882
Size: 5042
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 465 1330 1901 2867 3299 4046 4314 465
Size: 865 571 966 432 747 268 1193
Fragments arranged by size:
1193 966 865 747 571 432 268
AfeI AGC'GCT
Cuts at: 237 910 993 237
Size: 673 83 4286
Fragments arranged by size:
4286 673 83
AflIII A'CryG_T
Cuts at: 2394 3498 4475 2394
Size: 1104 977 2961
Fragments arranged by size:
2961 1104 977
AgeI A'CCGG_T
Cuts at: 3430 3430
Size: 5042
AleI CACnn'nnGTG
Cuts at: 646 865 646
Size: 219 4823
AlwNI CAG_nnn'CTG
Cuts at: 327 393 1204 2347 3914 327
Size: 66 811 1143 1567 1455
Fragments arranged by size:
1567 1455 1143 811 66
ApaI G_GGCC'C
Cuts at: 755 1629 755
Size: 874 4168
ApaLI G'TGCA_C
Cuts at: 3812 3812
Size: 5042
ApoI r'AATT_y
Cuts at: 2046 4395 4651 2046
Size: 2349 256 2437
Fragments arranged by size:
2437 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5042
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5042
AvaI C'yCGr_G
Cuts at: 218 819 1126 1785 2062 218
Size: 601 307 659 277 3198
Fragments arranged by size:
3198 659 601 307 277
BamHI G'GATC_C
Cuts at: 2067 2067
Size: 5042
BanI G'GyrC_C
Cuts at: 263 1156 1242 1492 1702 2058 2472 2720 2755
Size: 893 86 250 210 356 414 248 35
Cuts at: 2755 263
Size: 2550
Fragments arranged by size:
2550 893 414 356 250 248 210 86
35
BanII G_rGCy'C
Cuts at: 755 1277 1354 1629 1740 2056 3086 755
Size: 522 77 275 111 316 1030 2711
Fragments arranged by size:
2711 1030 522 316 275 111 77
BbeI G_GCGC'C
Cuts at: 267 1496 2476 2724 267
Size: 1229 980 248 2585
Fragments arranged by size:
2585 1229 980 248
BbsI GAAGACnn'nnnn_
Cuts at: 423 1217 423
Size: 794 4248
BbvCI CC'TCA_GC
Cuts at: 239 1162 239
Size: 923 4119
BciVI GTATCCnnnnn_n'
Cuts at: 2940 3701 5042 2940
Size: 761 1341 2940
Fragments arranged by size:
2940 1341 761
BclI T'GATC_A
Cuts at: 2562 2562
Size: 5042
BglI GCCn_nnn'nGGC
Cuts at: 112 946 1757 112
Size: 834 811 3397
Fragments arranged by size:
3397 834 811
BglII A'GATC_T
Cuts at: 1110 2557 4594 1110
Size: 1447 2037 1558
Fragments arranged by size:
2037 1558 1447
BlpI GC'TnA_GC
Cuts at: 2154 2154
Size: 5042
Bme1580I G_kGCm'C
Cuts at: 755 1629 1707 1714 2667 2760 3816 755
Size: 874 78 7 953 93 1056 1981
Fragments arranged by size:
1981 1056 953 874 93 78 7
BmrI ACTGGGnnnn_n'
Cuts at: 2254 2335 2670 4814 2254
Size: 81 335 2144 2482
Fragments arranged by size:
2482 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3409 3409
Size: 5042
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 1139 1602 1987 490
Size: 649 463 385 3545
Fragments arranged by size:
3545 649 463 385
Bpu10I CC'TnA_GC
Cuts at: 239 1162 1635 1662 239
Size: 923 473 27 3619
Fragments arranged by size:
3619 923 473 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 2218 3097 3589 3887 346
Size: 1872 879 492 298 1501
Fragments arranged by size:
1872 1501 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 115 2009 4746 115
Size: 1894 2737 411
Fragments arranged by size:
2737 1894 411
BsaAI yAC'GTr
Cuts at: 915 2395 915
Size: 1480 3562
BsaBI GATnn'nnATC
Cuts at: 6 2556 4593 6
Size: 2550 2037 455
Fragments arranged by size:
2550 2037 455
BsaHI Gr'CG_yC
Cuts at: 264 604 1439 1493 1646 2473 2721 3423 4919
Size: 340 835 54 153 827 248 702 1496
Cuts at: 4919 264
Size: 387
Fragments arranged by size:
1496 835 827 702 387 340 248 153
54
BsaWI w'CCGG_w
Cuts at: 619 2231 2752 3430 3704 3851 4262 619
Size: 1612 521 678 274 147 411 1399
Fragments arranged by size:
1612 1399 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1325 1355 1325
Size: 30 5012
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 837 1036 1349 1591 837
Size: 199 313 242 4288
Fragments arranged by size:
4288 313 242 199
BseYI C'CCAG_C
Cuts at: 327 1389 1510 1630 2467 3225 3802 4298 327
Size: 1062 121 120 837 758 577 496 1071
Fragments arranged by size:
1071 1062 837 758 577 496 121 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 615 925 340
Size: 275 310 4457
Fragments arranged by size:
4457 310 275
BsiEI CG_ry'CG
Cuts at: 104 317 2584 2630 3838 104
Size: 213 2267 46 1208 1308
Fragments arranged by size:
2267 1308 1208 213 46
BsiHKAI G_wGCw'C
Cuts at: 2056 2834 3024 3816 2056
Size: 778 190 792 3282
Fragments arranged by size:
3282 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 755 1277 1354 1629 1707 1714 1740 2056 2667
Size: 522 77 275 78 7 26 316 611
Cuts at: 2667 2760 2834 3024 3086 3816 755
Size: 93 74 190 62 730 1981
Fragments arranged by size:
1981 730 611 522 316 275 190 93
78 77 74 62 26 7
BspEI T'CCGG_A
Cuts at: 4262 4262
Size: 5042
BspHI T'CATG_A
Cuts at: 4218 4218
Size: 5042
BspMI ACCTGCnnnn'nnnn_
Cuts at: 312 891 2092 2306 2608 2989 312
Size: 579 1201 214 302 381 2365
Fragments arranged by size:
2365 1201 579 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3334 3388 4872 5037 50
Size: 3284 54 1484 165 55
Fragments arranged by size:
3284 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2954 2954
Size: 5042
BsrFI r'CCGG_y
Cuts at: 260 541 561 1419 3040 3221 3430 260
Size: 281 20 858 1621 181 209 1872
Fragments arranged by size:
1872 1621 858 281 209 181 20
BsrGI T'GTAC_A
Cuts at: 536 536
Size: 5042
BssSI C'ACGA_G
Cuts at: 1255 3313 3671 1255
Size: 2058 358 2626
Fragments arranged by size:
2626 2058 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2419 2419
Size: 5042
BstBI TT'CG_AA
Cuts at: 1106 3403 1106
Size: 2297 2745
BstYI r'GATC_y
Cuts at: 1 1110 2067 2534 2557 2892 3138 4150 4236
Size: 1109 957 467 23 335 246 1012 86
Cuts at: 4236 4248 4594 1
Size: 12 346 449
Fragments arranged by size:
1109 1012 957 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 116 281 1307 107
Size: 9 165 1026 3842
Fragments arranged by size:
3842 1026 165 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2992 3173 3336 2992
Size: 181 163 4698
Fragments arranged by size:
4698 181 163
BtsI GCAGTG_nn'
Cuts at: 497 1324 2285 497
Size: 827 961 3254
Fragments arranged by size:
3254 961 827
DraI TTT'AAA
Cuts at: 2041 2041
Size: 5042
DraIII CAC_nnn'GTG
Cuts at: 733 733
Size: 5042
DrdI GACnn_nn'nnGTC
Cuts at: 160 1867 2748 3606 160
Size: 1707 881 858 1596
Fragments arranged by size:
1707 1596 881 858
EaeI y'GGCC_r
Cuts at: 314 1847 2627 2801 3192 3219 4564 314
Size: 1533 780 174 391 27 1345 792
Fragments arranged by size:
1533 1345 792 780 391 174 27
EagI C'GGCC_G
Cuts at: 314 2627 314
Size: 2313 2729
EarI CTCTTCn'nnn_
Cuts at: 424 3065 3275 424
Size: 2641 210 2191
Fragments arranged by size:
2641 2191 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3250 3560 3706 4852 3250
Size: 310 146 1146 3440
Fragments arranged by size:
3440 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2054 2054
Size: 5042
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 465 490 1139 1330 1602 1901 1987 2867 3299
Size: 25 649 191 272 299 86 880 432
Cuts at: 3299 4046 4314 465
Size: 747 268 1193
Fragments arranged by size:
1193 880 747 649 432 299 272 268
191 86 25
EcoO109I rG'GnC_Cy
Cuts at: 752 1530 1625 1626 1759 1803 1876 2181 752
Size: 778 95 1 133 44 73 305 3613
Fragments arranged by size:
3613 778 305 133 95 73 44 1
EcoRI G'AATT_C
Cuts at: 2046 2046
Size: 5042
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3061 3061
Size: 5042
FspI TGC'GCA
Cuts at: 2823 2823
Size: 5042
HaeII r_GCGC'y
Cuts at: 239 267 912 995 1471 1496 2476 2724 3746
Size: 28 645 83 476 25 980 248 1022
Cuts at: 3746 4725 239
Size: 979 556
Fragments arranged by size:
1022 980 979 645 556 476 248 83
28 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1325 1357 1385 1417 1727 1759 2884 2914 2916
Size: 32 28 32 310 32 1125 30 2
Cuts at: 2916 2946 1325
Size: 30 3421
Fragments arranged by size:
3421 1125 310 32 32 32 30 30
28 2
HincII GTy'rAC
Cuts at: 1572 2081 1572
Size: 509 4533
KasI G'GCGC_C
Cuts at: 263 1492 2472 2720 263
Size: 1229 980 248 2585
Fragments arranged by size:
2585 1229 980 248
KpnI G_GTAC'C
Cuts at: 2062 2062
Size: 5042
MluI A'CGCG_T
Cuts at: 4475 4475
Size: 5042
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 320 1977 3713 3897 320
Size: 1657 1736 184 1465
Fragments arranged by size:
1736 1657 1465 184
MscI TGG'CCA
Cuts at: 1849 2803 1849
Size: 954 4088
MslI CAynn'nnrTG
Cuts at: 646 742 865 1012 1018 1363 2006 3031 3158
Size: 96 123 147 6 345 643 1025 127
Cuts at: 3158 646
Size: 2530
Fragments arranged by size:
2530 1025 643 345 147 127 123 96
6
MspA1I CmG'CkG
Cuts at: 1379 1553 1748 2153 2467 2827 3840 4085 1379
Size: 174 195 405 314 360 1013 245 2336
Fragments arranged by size:
2336 1013 405 360 314 245 195 174
NaeI GCC'GGC
Cuts at: 262 1421 3223 262
Size: 1159 1802 2081
Fragments arranged by size:
2081 1802 1159
NarI GG'CG_CC
Cuts at: 264 1493 2473 2721 264
Size: 1229 980 248 2585
Fragments arranged by size:
2585 1229 980 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5042
NgoMIV G'CCGG_C
Cuts at: 260 1419 3221 260
Size: 1159 1802 2081
Fragments arranged by size:
2081 1802 1159
NspI r_CATG'y
Cuts at: 1083 2095 2595 3502 1083
Size: 1012 500 907 2623
Fragments arranged by size:
2623 1012 907 500
PasI CC'CwG_GG
Cuts at: 1707 1707
Size: 5042
PciI A'CATG_T
Cuts at: 3498 3498
Size: 5042
PflMI CCAn_nnn'nTGG
Cuts at: 693 693
Size: 5042
PfoI T'CCnGG_A
Cuts at: 787 1586 1853 787
Size: 799 267 3976
Fragments arranged by size:
3976 799 267
PmeI GTTT'AAAC
Cuts at: 2041 2041
Size: 5042
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4181 4213 4181
Size: 32 5010
PpuMI rG'GwC_Cy
Cuts at: 1530 1803 1876 1530
Size: 273 73 4696
Fragments arranged by size:
4696 273 73
PspOMI G'GGCC_C
Cuts at: 751 1625 751
Size: 874 4168
PstI C_TGCA'G
Cuts at: 1200 2089 2774 1200
Size: 889 685 3468
Fragments arranged by size:
3468 889 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5042
PvuII CAG'CTG
Cuts at: 1748 2467 2827 1748
Size: 719 360 3963
Fragments arranged by size:
3963 719 360
RsrII CG'GwC_CG
Cuts at: 3237 3237
Size: 5042
SacI G_AGCT'C
Cuts at: 2056 2056
Size: 5042
SalI G'TCGA_C
Cuts at: 2079 2079
Size: 5042
SapI GCTCTTCn'nnn_
Cuts at: 424 3065 3275 424
Size: 2641 210 2191
Fragments arranged by size:
2641 2191 210
SbfI CC_TGCA'GG
Cuts at: 2089 2089
Size: 5042
ScaI AGT'ACT
Cuts at: 4559 4559
Size: 5042
SexAI A'CCwGG_T
Cuts at: 962 962
Size: 5042
SfcI C'TryA_G
Cuts at: 33 1196 1205 2085 2770 3763 3954 33
Size: 1163 9 880 685 993 191 1121
Fragments arranged by size:
1163 1121 993 880 685 191 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 946 946
Size: 5042
SfoI GGC'GCC
Cuts at: 265 1494 2474 2722 265
Size: 1229 980 248 2585
Fragments arranged by size:
2585 1229 980 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5042
SmaI CCC'GGG
Cuts at: 1128 1787 2064 1128
Size: 659 277 4106
Fragments arranged by size:
4106 659 277
SmlI C'TyrA_G
Cuts at: 361 2197 3112 3604 3866 361
Size: 1836 915 492 262 1537
Fragments arranged by size:
1836 1537 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5042
SphI G_CATG'C
Cuts at: 2095 2595 2095
Size: 500 4542
StuI AGG'CCT
Cuts at: 346 346
Size: 5042
StyI C'CwwG_G
Cuts at: 107 1268 1487 1835 2176 2530 107
Size: 1161 219 348 341 354 2619
Fragments arranged by size:
2619 1161 354 348 341 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 390 844 1260 1470 1524 2625 2758 3217 3426
Size: 454 416 210 54 1101 133 459 209
Cuts at: 3426 390
Size: 2006
Fragments arranged by size:
2006 1101 459 454 416 210 209 133
54
TatI w'GTAC_w
Cuts at: 515 536 1076 4557 515
Size: 21 540 3481 1000
Fragments arranged by size:
3481 1000 540 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1424 1456 1977 2009 4559 4591 1424
Size: 32 521 32 2550 32 1875
Fragments arranged by size:
2550 1875 521 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2839 2839
Size: 5042
XbaI T'CTAG_A
Cuts at: 2073 2073
Size: 5042
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1638 1842 2537 114
Size: 1524 204 695 2619
Fragments arranged by size:
2619 1524 695 204
XmaI C'CCGG_G
Cuts at: 1126 1785 2062 1126
Size: 659 277 4106
Fragments arranged by size:
4106 659 277
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI