(Circular) (Six-base) MAPSORT of: pF1KE2742.seq Check: 5645 from: 1 to: 7196 pF1KE2742 7196 bp With 182 enzymes: SgfI * June 19, 2019 14:13 .. AatII G_ACGT'C Cuts at: 2990 2990 Size: 7196 AccI GT'mk_AC Cuts at: 1235 1802 2990 3680 4234 1235 Size: 567 1188 690 554 4197 Fragments arranged by size: 4197 1188 690 567 554 Acc65I G'GTAC_C Cuts at: 4212 4212 Size: 7196 AclI AA'CG_TT Cuts at: 7036 7036 Size: 7196 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 547 932 1759 2446 3430 3547 3882 5021 5453 Size: 385 827 687 984 117 335 1139 432 Cuts at: 5453 6200 6468 547 Size: 747 268 1275 Fragments arranged by size: 1275 1139 984 827 747 687 432 385 335 268 117 AflIII A'CryG_T Cuts at: 4548 5652 6629 4548 Size: 1104 977 5115 Fragments arranged by size: 5115 1104 977 AgeI A'CCGG_T Cuts at: 722 3240 5584 722 Size: 2518 2344 2334 Fragments arranged by size: 2518 2344 2334 AleI CACnn'nnGTG Cuts at: 617 1360 617 Size: 743 6453 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 313 313 Size: 7196 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 662 694 749 781 662 Size: 32 55 32 7077 Fragments arranged by size: 7077 55 32 32 AlwNI CAG_nnn'CTG Cuts at: 205 1286 1490 1758 2176 3635 4501 6068 205 Size: 1081 204 268 418 1459 866 1567 1333 Fragments arranged by size: 1567 1459 1333 1081 866 418 268 204 ApaI G_GGCC'C Cuts at: 2224 2677 2936 2224 Size: 453 259 6484 Fragments arranged by size: 6484 453 259 ApaLI G'TGCA_C Cuts at: 5966 5966 Size: 7196 ApoI r'AATT_y Cuts at: 1133 2275 2721 2738 4200 6549 6805 1133 Size: 1142 446 17 1462 2349 256 1524 Fragments arranged by size: 2349 1524 1462 1142 446 256 17 AseI AT'TA_AT Cuts at: 20 20 Size: 7196 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7196 AvaI C'yCGr_G Cuts at: 998 2432 4216 998 Size: 1434 1784 3978 Fragments arranged by size: 3978 1784 1434 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 1409 1442 1513 1546 1409 Size: 33 71 33 7059 Fragments arranged by size: 7059 71 33 33 BamHI G'GATC_C Cuts at: 510 797 3338 4221 510 Size: 287 2541 883 3485 Fragments arranged by size: 3485 2541 883 287 BanI G'GyrC_C Cuts at: 212 783 3053 3367 4212 4626 4874 4909 212 Size: 571 2270 314 845 414 248 35 2499 Fragments arranged by size: 2499 2270 845 571 414 314 248 35 BanII G_rGCy'C Cuts at: 229 2224 2677 2936 3353 4210 5240 229 Size: 1995 453 259 417 857 1030 2185 Fragments arranged by size: 2185 1995 1030 857 453 417 259 BbeI G_GCGC'C Cuts at: 4630 4878 4630 Size: 248 6948 BbsI GAAGACnn'nnnn_ Cuts at: 962 3426 962 Size: 2464 4732 BbvCI CC'TCA_GC Cuts at: 1219 1219 Size: 7196 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 369 403 2335 2369 369 Size: 34 1932 34 5196 Fragments arranged by size: 5196 1932 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5094 5855 7196 5094 Size: 761 1341 5094 Fragments arranged by size: 5094 1341 761 BclI T'GATC_A Cuts at: 1904 2057 2318 4716 1904 Size: 153 261 2398 4384 Fragments arranged by size: 4384 2398 261 153 BglI GCCn_nnn'nGGC Cuts at: 856 1329 2681 856 Size: 473 1352 5371 Fragments arranged by size: 5371 1352 473 BglII A'GATC_T Cuts at: 4711 6748 4711 Size: 2037 5159 BlpI GC'TnA_GC Cuts at: 4308 4308 Size: 7196 Bme1580I G_kGCm'C Cuts at: 217 444 788 1732 2224 2677 2936 4163 4821 Size: 227 344 944 492 453 259 1227 658 Cuts at: 4821 4914 5970 217 Size: 93 1056 1443 Fragments arranged by size: 1443 1227 1056 944 658 492 453 344 259 227 93 BmgBI CAC'GTC Cuts at: 1074 3684 1074 Size: 2610 4586 BmrI ACTGGGnnnn_n' Cuts at: 2278 3285 3507 3821 4408 4489 4824 6968 2278 Size: 1007 222 314 587 81 335 2144 2506 Fragments arranged by size: 2506 2144 1007 587 335 314 222 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1234 3367 5563 1234 Size: 2133 2196 2867 Fragments arranged by size: 2867 2196 2133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1433 2821 3102 3217 3987 1433 Size: 1388 281 115 770 4642 Fragments arranged by size: 4642 1388 770 281 115 Bpu10I CC'TnA_GC Cuts at: 1219 1219 Size: 7196 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 580 4039 4372 5251 5743 6041 580 Size: 3459 333 879 492 298 1735 Fragments arranged by size: 3459 1735 879 492 333 298 BsaI GGTCTCn'nnnn_ Cuts at: 4057 6900 4057 Size: 2843 4353 BsaAI yAC'GTr Cuts at: 2211 4549 2211 Size: 2338 4858 BsaBI GATnn'nnATC Cuts at: 6 4710 6747 6 Size: 4704 2037 455 Fragments arranged by size: 4704 2037 455 BsaHI Gr'CG_yC Cuts at: 2414 2987 3431 3626 4627 4875 5577 7073 2414 Size: 573 444 195 1001 248 702 1496 2537 Fragments arranged by size: 2537 1496 1001 702 573 444 248 195 BsaWI w'CCGG_w Cuts at: 722 3240 4385 4906 5584 5858 6005 6416 722 Size: 2518 1145 521 678 274 147 411 1502 Fragments arranged by size: 2518 1502 1145 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 662 692 1284 1314 2575 2605 3001 3031 662 Size: 30 592 30 1261 30 396 30 4827 Fragments arranged by size: 4827 1261 592 396 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 434 1561 2011 434 Size: 1127 450 5619 Fragments arranged by size: 5619 1127 450 BseYI C'CCAG_C Cuts at: 118 208 433 656 3178 4621 5379 5956 6452 Size: 90 225 223 2522 1443 758 577 496 Cuts at: 6452 118 Size: 862 Fragments arranged by size: 2522 1443 862 758 577 496 225 223 90 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 874 1519 2760 3621 874 Size: 645 1241 861 4449 Fragments arranged by size: 4449 1241 861 645 BsiEI CG_ry'CG Cuts at: 104 1275 2371 4738 4784 5992 104 Size: 1171 1096 2367 46 1208 1308 Fragments arranged by size: 2367 1308 1208 1171 1096 46 BsiHKAI G_wGCw'C Cuts at: 2261 2295 3220 4210 4988 5178 5970 2261 Size: 34 925 990 778 190 792 3487 Fragments arranged by size: 3487 990 925 792 778 190 34 BsmI GAATG_Cn' Cuts at: 4075 4075 Size: 7196 BsmBI CGTCTCn'nnnn_ Cuts at: 4005 4005 Size: 7196 Bsp1286I G_dGCh'C Cuts at: 217 229 444 788 1732 2224 2261 2295 2677 Size: 12 215 344 944 492 37 34 382 Cuts at: 2677 2936 3220 3353 4163 4210 4821 4914 4988 Size: 259 284 133 810 47 611 93 74 Cuts at: 4988 5178 5240 5970 217 Size: 190 62 730 1443 Fragments arranged by size: 1443 944 810 730 611 492 382 344 284 259 215 190 133 93 74 62 47 37 34 12 BspEI T'CCGG_A Cuts at: 6416 6416 Size: 7196 BspHI T'CATG_A Cuts at: 1098 6372 1098 Size: 5274 1922 BspMI ACCTGCnnnn'nnnn_ Cuts at: 184 1541 3155 4246 4460 4762 5143 184 Size: 1357 1614 1091 214 302 381 2237 Fragments arranged by size: 2237 1614 1357 1091 381 302 214 BsrBI CCG'CTC Cuts at: 50 5488 5542 7026 7191 50 Size: 5438 54 1484 165 55 Fragments arranged by size: 5438 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1427 2115 2459 2879 5108 1427 Size: 688 344 420 2229 3515 Fragments arranged by size: 3515 2229 688 420 344 BsrFI r'CCGG_y Cuts at: 722 1792 2439 2525 2807 2875 3240 5194 5375 Size: 1070 647 86 282 68 365 1954 181 Cuts at: 5375 5584 722 Size: 209 2334 Fragments arranged by size: 2334 1954 1070 647 365 282 209 181 86 68 BsrGI T'GTAC_A Cuts at: 1395 3119 1395 Size: 1724 5472 BssSI C'ACGA_G Cuts at: 3891 5467 5825 3891 Size: 1576 358 5262 Fragments arranged by size: 5262 1576 358 BstAPI GCAn_nnn'nTGC Cuts at: 1286 1490 2048 3104 4573 1286 Size: 204 558 1056 1469 3909 Fragments arranged by size: 3909 1469 1056 558 204 BstBI TT'CG_AA Cuts at: 5557 5557 Size: 7196 BstXI CCAn_nnnn'nTGG Cuts at: 1203 1993 2823 4026 4170 1203 Size: 790 830 1203 144 4229 Fragments arranged by size: 4229 1203 830 790 144 BstYI r'GATC_y Cuts at: 1 510 739 797 1486 2173 2666 3338 4221 Size: 509 229 58 689 687 493 672 883 Cuts at: 4221 4688 4711 5046 5292 6304 6390 6402 6748 Size: 467 23 335 246 1012 86 12 346 Cuts at: 6748 1 Size: 449 Fragments arranged by size: 1012 883 689 687 672 509 493 467 449 346 335 246 229 86 58 23 12 BtgI C'CryG_G Cuts at: 107 317 666 1884 2228 2481 3925 4012 107 Size: 210 349 1218 344 253 1444 87 3291 Fragments arranged by size: 3291 1444 1218 349 344 253 210 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 874 5146 5327 5490 874 Size: 4272 181 163 2580 Fragments arranged by size: 4272 2580 181 163 BtsI GCAGTG_nn' Cuts at: 295 818 1938 3105 4439 295 Size: 523 1120 1167 1334 3052 Fragments arranged by size: 3052 1334 1167 1120 523 DraI TTT'AAA Cuts at: 4195 4195 Size: 7196 DraIII CAC_nnn'GTG Cuts at: 273 273 Size: 7196 DrdI GACnn_nn'nnGTC Cuts at: 2332 4902 5760 2332 Size: 2570 858 3768 Fragments arranged by size: 3768 2570 858 EaeI y'GGCC_r Cuts at: 252 282 848 1790 2132 2368 2441 2558 4781 Size: 30 566 942 342 236 73 117 2223 Cuts at: 4781 4955 5346 5373 6718 252 Size: 174 391 27 1345 730 Fragments arranged by size: 2223 1345 942 730 566 391 342 236 174 117 73 30 27 EagI C'GGCC_G Cuts at: 2368 4781 2368 Size: 2413 4783 EarI CTCTTCn'nnn_ Cuts at: 142 315 1457 5219 5429 142 Size: 173 1142 3762 210 1909 Fragments arranged by size: 3762 1909 1142 210 173 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1588 2821 5404 5714 5860 7006 1588 Size: 1233 2583 310 146 1146 1778 Fragments arranged by size: 2583 1778 1233 1146 310 146 EcoICRI GAG'CTC Cuts at: 4208 4208 Size: 7196 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 547 932 1433 1759 2446 2821 3102 3217 3430 Size: 385 501 326 687 375 281 115 213 Cuts at: 3430 3547 3882 3987 5021 5453 6200 6468 547 Size: 117 335 105 1034 432 747 268 1275 Fragments arranged by size: 1275 1034 747 687 501 432 385 375 335 326 281 268 213 117 115 105 EcoNI CCTnn'n_nnAGG Cuts at: 139 3256 139 Size: 3117 4079 EcoO109I rG'GnC_Cy Cuts at: 1842 2221 2486 2673 2674 2702 2933 3443 4335 Size: 379 265 187 1 28 231 510 892 Cuts at: 4335 1842 Size: 4703 Fragments arranged by size: 4703 892 510 379 265 231 187 28 1 EcoRI G'AATT_C Cuts at: 2738 4200 2738 Size: 1462 5734 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2631 3189 3720 3786 5215 2631 Size: 558 531 66 1429 4612 Fragments arranged by size: 4612 1429 558 531 66 FspI TGC'GCA Cuts at: 4977 4977 Size: 7196 HaeII r_GCGC'y Cuts at: 557 2822 3208 4630 4878 5900 6879 557 Size: 2265 386 1422 248 1022 979 874 Fragments arranged by size: 2265 1422 1022 979 874 386 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 682 714 744 776 1093 1125 2121 2153 2575 Size: 32 30 32 317 32 996 32 422 Cuts at: 2575 2607 2956 2988 3493 3525 3588 3620 5038 Size: 32 349 32 505 32 63 32 1418 Cuts at: 5038 5068 5070 5100 682 Size: 30 2 30 2778 Fragments arranged by size: 2778 1418 996 505 422 349 317 63 32 32 32 32 32 32 32 32 30 30 30 2 HincII GTy'rAC Cuts at: 1215 2991 4235 1215 Size: 1776 1244 4176 Fragments arranged by size: 4176 1776 1244 HindIII A'AGCT_T Cuts at: 4033 4033 Size: 7196 HpaI GTT'AAC Cuts at: 1215 1215 Size: 7196 KasI G'GCGC_C Cuts at: 4626 4874 4626 Size: 248 6948 KpnI G_GTAC'C Cuts at: 4216 4216 Size: 7196 MluI A'CGCG_T Cuts at: 6629 6629 Size: 7196 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2324 5867 6051 2324 Size: 3543 184 3469 Fragments arranged by size: 3543 3469 184 MscI TGG'CCA Cuts at: 254 850 2134 2560 4957 254 Size: 596 1284 426 2397 2493 Fragments arranged by size: 2493 2397 1284 596 426 MslI CAynn'nnrTG Cuts at: 617 1201 1360 1603 1606 3805 3847 4111 4168 Size: 584 159 243 3 2199 42 264 57 Cuts at: 4168 5185 5312 617 Size: 1017 127 2501 Fragments arranged by size: 2501 2199 1017 584 264 243 159 127 57 42 3 MspA1I CmG'CkG Cuts at: 161 1758 2167 2424 2522 2949 3717 4307 4621 Size: 1597 409 257 98 427 768 590 314 Cuts at: 4621 4981 5994 6239 161 Size: 360 1013 245 1118 Fragments arranged by size: 1597 1118 1013 768 590 427 409 360 314 257 245 98 NaeI GCC'GGC Cuts at: 2441 2809 5377 2441 Size: 368 2568 4260 Fragments arranged by size: 4260 2568 368 NarI GG'CG_CC Cuts at: 4627 4875 4627 Size: 248 6948 NcoI C'CATG_G Cuts at: 107 1884 3925 4012 107 Size: 1777 2041 87 3291 Fragments arranged by size: 3291 2041 1777 87 NdeI CA'TA_TG Cuts at: 1745 1745 Size: 7196 NgoMIV G'CCGG_C Cuts at: 2439 2807 5375 2439 Size: 368 2568 4260 Fragments arranged by size: 4260 2568 368 NspI r_CATG'y Cuts at: 433 1569 4249 4749 5656 433 Size: 1136 2680 500 907 1973 Fragments arranged by size: 2680 1973 1136 907 500 PasI CC'CwG_GG Cuts at: 184 2077 184 Size: 1893 5303 PciI A'CATG_T Cuts at: 5652 5652 Size: 7196 PflMI CCAn_nnn'nTGG Cuts at: 161 1061 2126 3275 3738 3852 161 Size: 900 1065 1149 463 114 3505 Fragments arranged by size: 3505 1149 1065 900 463 114 PfoI T'CCnGG_A Cuts at: 586 586 Size: 7196 PmeI GTTT'AAAC Cuts at: 4195 4195 Size: 7196 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 662 694 6335 6367 662 Size: 32 5641 32 1491 Fragments arranged by size: 5641 1491 32 32 PpuMI rG'GwC_Cy Cuts at: 1842 2702 3443 1842 Size: 860 741 5595 Fragments arranged by size: 5595 860 741 PshAI GACnn'nnGTC Cuts at: 1988 3430 4049 1988 Size: 1442 619 5135 Fragments arranged by size: 5135 1442 619 PspOMI G'GGCC_C Cuts at: 2220 2673 2932 2220 Size: 453 259 6484 Fragments arranged by size: 6484 453 259 PstI C_TGCA'G Cuts at: 1486 2172 3257 4243 4928 1486 Size: 686 1085 986 685 3754 Fragments arranged by size: 3754 1085 986 686 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 7196 PvuII CAG'CTG Cuts at: 161 1758 2522 2949 3717 4621 4981 161 Size: 1597 764 427 768 904 360 2376 Fragments arranged by size: 2376 1597 904 768 764 427 360 RsrII CG'GwC_CG Cuts at: 2031 5391 2031 Size: 3360 3836 SacI G_AGCT'C Cuts at: 4210 4210 Size: 7196 SalI G'TCGA_C Cuts at: 2989 4233 2989 Size: 1244 5952 SapI GCTCTTCn'nnn_ Cuts at: 5219 5429 5219 Size: 210 6986 SbfI CC_TGCA'GG Cuts at: 4243 4243 Size: 7196 ScaI AGT'ACT Cuts at: 1437 6713 1437 Size: 5276 1920 SexAI A'CCwGG_T Cuts at: 1502 1502 Size: 7196 SfcI C'TryA_G Cuts at: 33 735 1482 1638 2168 2711 3253 4239 4924 Size: 702 747 156 530 543 542 986 685 Cuts at: 4924 5917 6108 33 Size: 993 191 1121 Fragments arranged by size: 1121 993 986 747 702 685 543 542 530 191 156 SfoI GGC'GCC Cuts at: 4628 4876 4628 Size: 248 6948 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7196 SmaI CCC'GGG Cuts at: 1000 2434 4218 1000 Size: 1434 1784 3978 Fragments arranged by size: 3978 1784 1434 SmlI C'TyrA_G Cuts at: 559 4054 4351 5266 5758 6020 559 Size: 3495 297 915 492 262 1735 Fragments arranged by size: 3495 1735 915 492 297 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 7196 SphI G_CATG'C Cuts at: 433 4249 4749 433 Size: 3816 500 2880 Fragments arranged by size: 3816 2880 500 SspI AAT'ATT Cuts at: 1036 1036 Size: 7196 StuI AGG'CCT Cuts at: 2350 2350 Size: 7196 StyI C'CwwG_G Cuts at: 107 894 910 1884 2357 3870 3925 4012 4330 Size: 787 16 974 473 1513 55 87 318 Cuts at: 4330 4684 107 Size: 354 2619 Fragments arranged by size: 2619 1513 974 787 473 354 318 87 55 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 200 629 1172 1842 3071 3092 3235 4101 4779 Size: 429 543 670 1229 21 143 866 678 Cuts at: 4779 4912 5371 5580 200 Size: 133 459 209 1816 Fragments arranged by size: 1816 1229 866 678 670 543 459 429 209 143 133 21 TatI w'GTAC_w Cuts at: 530 1395 1435 2237 3119 6711 530 Size: 865 40 802 882 3592 1015 Fragments arranged by size: 3592 1015 882 865 802 40 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1284 1316 6713 6745 1284 Size: 32 5397 32 1735 Fragments arranged by size: 5397 1735 32 32 Tth111I GACn'n_nGTC Cuts at: 4993 4993 Size: 7196 XbaI T'CTAG_A Cuts at: 4227 4227 Size: 7196 XcmI CCAnnnn_n'nnnnTGG Cuts at: 198 1024 1380 1891 2127 3975 4691 198 Size: 826 356 511 236 1848 716 2703 Fragments arranged by size: 2703 1848 826 716 511 356 236 XmaI C'CCGG_G Cuts at: 998 2432 4216 998 Size: 1434 1784 3978 Fragments arranged by size: 3978 1784 1434 XmnI GAAnn'nnTTC Cuts at: 500 3172 3790 500 Size: 2672 618 3906 Fragments arranged by size: 3906 2672 618 ZraI GAC'GTC Cuts at: 2988 2988 Size: 7196 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AflII AhdI AscI AvrII BfrBI BmtI BsiWI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI MfeI NheI NotI NruI NsiI PacI PmlI PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI