(Circular) (Six-base) MAPSORT of: pF1KE2751.seq Check: 7964 from: 1 to: 3929 pF1KE2751 3929 bp With 182 enzymes: SgfI * June 30, 2019 14:46 .. AccI GT'mk_AC Cuts at: 967 967 Size: 3929 Acc65I G'GTAC_C Cuts at: 945 945 Size: 3929 AclI AA'CG_TT Cuts at: 215 3769 215 Size: 3554 375 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 641 1754 2186 2933 3201 641 Size: 1113 432 747 268 1369 Fragments arranged by size: 1369 1113 747 432 268 AflIII A'CryG_T Cuts at: 601 921 1281 2385 3362 601 Size: 320 360 1104 977 1168 Fragments arranged by size: 1168 1104 977 360 320 AgeI A'CCGG_T Cuts at: 2317 2317 Size: 3929 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 357 389 357 Size: 32 3897 AlwNI CAG_nnn'CTG Cuts at: 1234 2801 1234 Size: 1567 2362 ApaLI G'TGCA_C Cuts at: 544 2699 544 Size: 2155 1774 ApoI r'AATT_y Cuts at: 432 933 3282 3538 432 Size: 501 2349 256 823 Fragments arranged by size: 2349 823 501 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3929 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3929 AvaI C'yCGr_G Cuts at: 182 949 182 Size: 767 3162 BamHI G'GATC_C Cuts at: 954 954 Size: 3929 BanI G'GyrC_C Cuts at: 191 945 1359 1607 1642 191 Size: 754 414 248 35 2478 Fragments arranged by size: 2478 754 414 248 35 BanII G_rGCy'C Cuts at: 943 1973 943 Size: 1030 2899 BbeI G_GCGC'C Cuts at: 195 1363 1611 195 Size: 1168 248 2513 Fragments arranged by size: 2513 1168 248 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 3895 BciVI GTATCCnnnnn_n' Cuts at: 1827 2588 3929 1827 Size: 761 1341 1827 Fragments arranged by size: 1827 1341 761 BclI T'GATC_A Cuts at: 1449 1449 Size: 3929 BfrBI ATG'CAT Cuts at: 207 207 Size: 3929 BglI GCCn_nnn'nGGC Cuts at: 236 236 Size: 3929 BglII A'GATC_T Cuts at: 1444 3481 1444 Size: 2037 1892 BlpI GC'TnA_GC Cuts at: 1041 1041 Size: 3929 Bme1580I G_kGCm'C Cuts at: 548 1554 1647 2703 548 Size: 1006 93 1056 1774 Fragments arranged by size: 1774 1056 1006 93 BmrI ACTGGGnnnn_n' Cuts at: 1141 1222 1557 3701 1141 Size: 81 335 2144 1369 Fragments arranged by size: 2144 1369 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 352 2296 352 Size: 1944 1985 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1105 1984 2476 2774 1105 Size: 879 492 298 2260 Fragments arranged by size: 2260 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3633 3633 Size: 3929 BsaAI yAC'GTr Cuts at: 1282 1282 Size: 3929 BsaBI GATnn'nnATC Cuts at: 6 1443 3480 6 Size: 1437 2037 455 Fragments arranged by size: 2037 1437 455 BsaHI Gr'CG_yC Cuts at: 192 1360 1608 2310 3806 192 Size: 1168 248 702 1496 315 Fragments arranged by size: 1496 1168 702 315 248 BsaWI w'CCGG_w Cuts at: 1118 1639 2317 2591 2738 3149 1118 Size: 521 678 274 147 411 1898 Fragments arranged by size: 1898 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 1354 2112 2689 3185 1354 Size: 758 577 496 2098 Fragments arranged by size: 2098 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 423 423 Size: 3929 BsiEI CG_ry'CG Cuts at: 104 1471 1517 2725 104 Size: 1367 46 1208 1308 Fragments arranged by size: 1367 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 548 943 1721 1911 2703 548 Size: 395 778 190 792 1774 Fragments arranged by size: 1774 792 778 395 190 BsmI GAATG_Cn' Cuts at: 209 209 Size: 3929 Bsp1286I G_dGCh'C Cuts at: 548 943 1554 1647 1721 1911 1973 2703 548 Size: 395 611 93 74 190 62 730 1774 Fragments arranged by size: 1774 730 611 395 190 93 74 62 BspEI T'CCGG_A Cuts at: 3149 3149 Size: 3929 BspHI T'CATG_A Cuts at: 3105 3105 Size: 3929 BspMI ACCTGCnnnn'nnnn_ Cuts at: 979 1193 1495 1876 979 Size: 214 302 381 3032 Fragments arranged by size: 3032 381 302 214 BsrBI CCG'CTC Cuts at: 50 2221 2275 3759 3924 50 Size: 2171 54 1484 165 55 Fragments arranged by size: 2171 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1841 1841 Size: 3929 BsrFI r'CCGG_y Cuts at: 1927 2108 2317 1927 Size: 181 209 3539 Fragments arranged by size: 3539 209 181 BsrGI T'GTAC_A Cuts at: 391 703 918 391 Size: 312 215 3402 Fragments arranged by size: 3402 312 215 BssSI C'ACGA_G Cuts at: 2200 2558 2200 Size: 358 3571 BstAPI GCAn_nnn'nTGC Cuts at: 1306 1306 Size: 3929 BstBI TT'CG_AA Cuts at: 2290 2290 Size: 3929 BstEII G'GTnAC_C Cuts at: 667 667 Size: 3929 BstYI r'GATC_y Cuts at: 1 954 1421 1444 1779 2025 3037 3123 3135 Size: 953 467 23 335 246 1012 86 12 Cuts at: 3135 3481 1 Size: 346 449 Fragments arranged by size: 1012 953 467 449 346 335 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1879 2060 2223 1879 Size: 181 163 3585 Fragments arranged by size: 3585 181 163 BtsI GCAGTG_nn' Cuts at: 1172 1172 Size: 3929 DraI TTT'AAA Cuts at: 928 928 Size: 3929 DrdI GACnn_nn'nnGTC Cuts at: 1635 2493 1635 Size: 858 3071 EaeI y'GGCC_r Cuts at: 1514 1688 2079 2106 3451 1514 Size: 174 391 27 1345 1992 Fragments arranged by size: 1992 1345 391 174 27 EagI C'GGCC_G Cuts at: 1514 1514 Size: 3929 EarI CTCTTCn'nnn_ Cuts at: 345 1952 2162 345 Size: 1607 210 2112 Fragments arranged by size: 2112 1607 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2137 2447 2593 3739 2137 Size: 310 146 1146 2327 Fragments arranged by size: 2327 1146 310 146 EcoICRI GAG'CTC Cuts at: 941 941 Size: 3929 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 641 1754 2186 2933 3201 641 Size: 1113 432 747 268 1369 Fragments arranged by size: 1369 1113 747 432 268 EcoNI CCTnn'n_nnAGG Cuts at: 779 779 Size: 3929 EcoO109I rG'GnC_Cy Cuts at: 1068 1068 Size: 3929 EcoRI G'AATT_C Cuts at: 933 933 Size: 3929 EcoRV GAT'ATC Cuts at: 373 373 Size: 3929 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1948 1948 Size: 3929 FspI TGC'GCA Cuts at: 1710 1710 Size: 3929 HaeII r_GCGC'y Cuts at: 195 1363 1611 2633 3612 195 Size: 1168 248 1022 979 512 Fragments arranged by size: 1168 1022 979 512 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1771 1801 1803 1833 1771 Size: 30 2 30 3867 Fragments arranged by size: 3867 30 30 2 HincII GTy'rAC Cuts at: 968 968 Size: 3929 KasI G'GCGC_C Cuts at: 191 1359 1607 191 Size: 1168 248 2513 Fragments arranged by size: 2513 1168 248 KpnI G_GTAC'C Cuts at: 949 949 Size: 3929 MfeI C'AATT_G Cuts at: 489 489 Size: 3929 MluI A'CGCG_T Cuts at: 3362 3362 Size: 3929 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 485 2600 2784 485 Size: 2115 184 1630 Fragments arranged by size: 2115 1630 184 MscI TGG'CCA Cuts at: 1690 1690 Size: 3929 MslI CAynn'nnrTG Cuts at: 1918 2045 1918 Size: 127 3802 MspA1I CmG'CkG Cuts at: 1040 1354 1714 2727 2972 1040 Size: 314 360 1013 245 1997 Fragments arranged by size: 1997 1013 360 314 245 NaeI GCC'GGC Cuts at: 2110 2110 Size: 3929 NarI GG'CG_CC Cuts at: 192 1360 1608 192 Size: 1168 248 2513 Fragments arranged by size: 2513 1168 248 NgoMIV G'CCGG_C Cuts at: 2108 2108 Size: 3929 NsiI A_TGCA'T Cuts at: 209 209 Size: 3929 NspI r_CATG'y Cuts at: 251 605 982 1482 2389 251 Size: 354 377 500 907 1791 Fragments arranged by size: 1791 907 500 377 354 PciI A'CATG_T Cuts at: 601 2385 601 Size: 1784 2145 PflMI CCAn_nnn'nTGG Cuts at: 525 525 Size: 3929 PmeI GTTT'AAAC Cuts at: 928 928 Size: 3929 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3068 3100 3068 Size: 32 3897 PstI C_TGCA'G Cuts at: 976 1661 976 Size: 685 3244 PvuI CG_AT'CG Cuts at: 104 104 Size: 3929 PvuII CAG'CTG Cuts at: 1354 1714 1354 Size: 360 3569 RsrII CG'GwC_CG Cuts at: 2124 2124 Size: 3929 SacI G_AGCT'C Cuts at: 943 943 Size: 3929 SalI G'TCGA_C Cuts at: 966 966 Size: 3929 SapI GCTCTTCn'nnn_ Cuts at: 1952 2162 1952 Size: 210 3719 SbfI CC_TGCA'GG Cuts at: 976 976 Size: 3929 ScaI AGT'ACT Cuts at: 3446 3446 Size: 3929 SfcI C'TryA_G Cuts at: 33 972 1657 2650 2841 33 Size: 939 685 993 191 1121 Fragments arranged by size: 1121 993 939 685 191 SfoI GGC'GCC Cuts at: 193 1361 1609 193 Size: 1168 248 2513 Fragments arranged by size: 2513 1168 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3929 SmaI CCC'GGG Cuts at: 184 951 184 Size: 767 3162 SmlI C'TyrA_G Cuts at: 1084 1999 2491 2753 1084 Size: 915 492 262 2260 Fragments arranged by size: 2260 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3929 SphI G_CATG'C Cuts at: 982 1482 982 Size: 500 3429 StyI C'CwwG_G Cuts at: 231 471 571 1063 1417 231 Size: 240 100 492 354 2743 Fragments arranged by size: 2743 492 354 240 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1512 1645 2104 2313 1512 Size: 133 459 209 3128 Fragments arranged by size: 3128 459 209 133 TatI w'GTAC_w Cuts at: 391 703 918 3444 391 Size: 312 215 2526 876 Fragments arranged by size: 2526 876 312 215 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3446 3478 3446 Size: 32 3897 Tth111I GACn'n_nGTC Cuts at: 1726 1726 Size: 3929 XbaI T'CTAG_A Cuts at: 960 960 Size: 3929 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1424 1424 Size: 3929 XmaI C'CCGG_G Cuts at: 182 949 182 Size: 767 3162 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI ApaI AscI AvrII BaeI BbsI BbvCI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmBI BssHII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII FseI FspAI HindIII HpaI NcoI NdeI NheI NotI NruI PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI