Restriction map for
pF1KE2752
(Circular) (Six-base) MAPSORT of: pF1KE2752.seq Check: 4268 from: 1 to: 6599
pF1KE2752 6599 bp
With 182 enzymes: SgfI *
August 1, 2019 16:47 ..
AccI GT'mk_AC
Cuts at: 1352 3637 1352
Size: 2285 4314
Acc65I G'GTAC_C
Cuts at: 654 1603 3615 654
Size: 949 2012 3638
Fragments arranged by size:
3638 2012 949
AclI AA'CG_TT
Cuts at: 6439 6439
Size: 6599
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 1825 2467 2672 2926 2953 3214 4424 4856
Size: 1554 642 205 254 27 261 1210 432
Cuts at: 4856 5603 5871 271
Size: 747 268 999
Fragments arranged by size:
1554 1210 999 747 642 432 268 261
254 205 27
AflII C'TTAA_G
Cuts at: 151 151
Size: 6599
AflIII A'CryG_T
Cuts at: 197 212 3951 5055 6032 197
Size: 15 3739 1104 977 764
Fragments arranged by size:
3739 1104 977 764 15
AgeI A'CCGG_T
Cuts at: 4987 4987
Size: 6599
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 231 241 231
Size: 10 6589
AlwNI CAG_nnn'CTG
Cuts at: 730 1455 2868 3538 3904 5471 730
Size: 725 1413 670 366 1567 1858
Fragments arranged by size:
1858 1567 1413 725 670 366
ApaLI G'TGCA_C
Cuts at: 5369 5369
Size: 6599
ApoI r'AATT_y
Cuts at: 1883 2623 3123 3410 3603 5952 6208 1883
Size: 740 500 287 193 2349 256 2274
Fragments arranged by size:
2349 2274 740 500 287 256 193
AseI AT'TA_AT
Cuts at: 20 20
Size: 6599
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6599
AvaI C'yCGr_G
Cuts at: 2342 3619 2342
Size: 1277 5322
BamHI G'GATC_C
Cuts at: 393 1660 2919 3624 393
Size: 1267 1259 705 3368
Fragments arranged by size:
3368 1267 1259 705
BanI G'GyrC_C
Cuts at: 654 1575 1603 2269 3034 3615 4029 4277 4312
Size: 921 28 666 765 581 414 248 35
Cuts at: 4312 654
Size: 2941
Fragments arranged by size:
2941 921 765 666 581 414 248 35
28
BanII G_rGCy'C
Cuts at: 1213 2304 3613 4643 1213
Size: 1091 1309 1030 3169
Fragments arranged by size:
3169 1309 1091 1030
BbeI G_GCGC'C
Cuts at: 4033 4281 4033
Size: 248 6351
BbvCI CC'TCA_GC
Cuts at: 2560 2560
Size: 6599
BciVI GTATCCnnnnn_n'
Cuts at: 429 4497 5258 6599 429
Size: 4068 761 1341 429
Fragments arranged by size:
4068 1341 761 429
BclI T'GATC_A
Cuts at: 1436 2183 4119 1436
Size: 747 1936 3916
Fragments arranged by size:
3916 1936 747
BfrBI ATG'CAT
Cuts at: 341 439 341
Size: 98 6501
BglI GCCn_nnn'nGGC
Cuts at: 1038 2392 1038
Size: 1354 5245
BglII A'GATC_T
Cuts at: 3482 4114 6151 3482
Size: 632 2037 3930
Fragments arranged by size:
3930 2037 632
BlpI GC'TnA_GC
Cuts at: 3711 3711
Size: 6599
Bme1580I G_kGCm'C
Cuts at: 2272 2388 4224 4317 5373 2272
Size: 116 1836 93 1056 3498
Fragments arranged by size:
3498 1836 1056 116 93
BmgBI CAC'GTC
Cuts at: 1994 1994
Size: 6599
BmrI ACTGGGnnnn_n'
Cuts at: 978 1330 2856 3811 3892 4227 6371 978
Size: 352 1526 955 81 335 2144 1206
Fragments arranged by size:
2144 1526 1206 955 352 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2140 2289 4966 2140
Size: 149 2677 3773
Fragments arranged by size:
3773 2677 149
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 752 1103 1477 1676 2806 3264 3362 3560 752
Size: 351 374 199 1130 458 98 198 3791
Fragments arranged by size:
3791 1130 458 374 351 199 198 98
Bpu10I CC'TnA_GC
Cuts at: 1097 1905 2560 1097
Size: 808 655 5136
Fragments arranged by size:
5136 808 655
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1777 3775 4654 5146 5444 1777
Size: 1998 879 492 298 2932
Fragments arranged by size:
2932 1998 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1054 6303 1054
Size: 5249 1350
BsaAI yAC'GTr
Cuts at: 198 215 2152 3952 198
Size: 17 1937 1800 2845
Fragments arranged by size:
2845 1937 1800 17
BsaBI GATnn'nnATC
Cuts at: 6 4113 6150 6
Size: 4107 2037 455
Fragments arranged by size:
4107 2037 455
BsaHI Gr'CG_yC
Cuts at: 161 4030 4278 4980 6476 161
Size: 3869 248 702 1496 284
Fragments arranged by size:
3869 1496 702 284 248
BsaWI w'CCGG_w
Cuts at: 3788 4309 4987 5261 5408 5819 3788
Size: 521 678 274 147 411 4568
Fragments arranged by size:
4568 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1548 1578 2779 2809 3533 3563 1548
Size: 30 1201 30 724 30 4584
Fragments arranged by size:
4584 1201 724 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1183 2505 2537 2577 2815 1183
Size: 1322 32 40 238 4967
Fragments arranged by size:
4967 1322 238 40 32
BseYI C'CCAG_C
Cuts at: 884 2615 4024 4782 5359 5855 884
Size: 1731 1409 758 577 496 1628
Fragments arranged by size:
1731 1628 1409 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 1088 2368 237
Size: 851 1280 4468
Fragments arranged by size:
4468 1280 851
BsiEI CG_ry'CG
Cuts at: 104 1057 4141 4187 5395 104
Size: 953 3084 46 1208 1308
Fragments arranged by size:
3084 1308 1208 953 46
BsiHKAI G_wGCw'C
Cuts at: 134 1387 3079 3613 4391 4581 5373 134
Size: 1253 1692 534 778 190 792 1360
Fragments arranged by size:
1692 1360 1253 792 778 534 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1237 1768 1237
Size: 531 6068
Bsp1286I G_dGCh'C
Cuts at: 134 1213 1387 2272 2304 2388 3079 3613 4224
Size: 1079 174 885 32 84 691 534 611
Cuts at: 4224 4317 4391 4581 4643 5373 134
Size: 93 74 190 62 730 1360
Fragments arranged by size:
1360 1079 885 730 691 611 534 190
174 93 84 74 62 32
BspEI T'CCGG_A
Cuts at: 5819 5819
Size: 6599
BspHI T'CATG_A
Cuts at: 5775 5775
Size: 6599
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3649 3863 4165 4546 3649
Size: 214 302 381 5702
Fragments arranged by size:
5702 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1053 4891 4945 6429 6594 50
Size: 1003 3838 54 1484 165 55
Fragments arranged by size:
3838 1484 1003 165 55 54
BsrDI GCAATG_nn'
Cuts at: 252 3184 4511 252
Size: 2932 1327 2340
Fragments arranged by size:
2932 2340 1327
BsrFI r'CCGG_y
Cuts at: 157 4597 4778 4987 157
Size: 4440 181 209 1769
Fragments arranged by size:
4440 1769 209 181
BssSI C'ACGA_G
Cuts at: 2200 4870 5228 2200
Size: 2670 358 3571
Fragments arranged by size:
3571 2670 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3976 3976
Size: 6599
BstBI TT'CG_AA
Cuts at: 2289 4960 2289
Size: 2671 3928
BstEII G'GTnAC_C
Cuts at: 879 879
Size: 6599
BstXI CCAn_nnnn'nTGG
Cuts at: 518 518
Size: 6599
BstYI r'GATC_y
Cuts at: 1 393 1660 1791 2240 2836 2919 3482 3624
Size: 392 1267 131 449 596 83 563 142
Cuts at: 3624 4091 4114 4449 4695 5707 5793 5805 6151
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6151 1
Size: 449
Fragments arranged by size:
1267 1012 596 563 467 449 449 392
346 335 246 142 131 86 83 23
12
Bsu36I CC'TnA_GG
Cuts at: 1034 1302 3290 1034
Size: 268 1988 4343
Fragments arranged by size:
4343 1988 268
BtgI C'CryG_G
Cuts at: 107 385 2031 2130 107
Size: 278 1646 99 4576
Fragments arranged by size:
4576 1646 278 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 182 1732 4549 4730 4893 182
Size: 1550 2817 181 163 1888
Fragments arranged by size:
2817 1888 1550 181 163
BtsI GCAGTG_nn'
Cuts at: 1164 1715 3842 1164
Size: 551 2127 3921
Fragments arranged by size:
3921 2127 551
ClaI AT'CG_AT
Cuts at: 3224 3224
Size: 6599
DraI TTT'AAA
Cuts at: 2642 3598 2642
Size: 956 5643
DraIII CAC_nnn'GTG
Cuts at: 1071 1575 1071
Size: 504 6095
DrdI GACnn_nn'nnGTC
Cuts at: 3577 4305 5163 3577
Size: 728 858 5013
Fragments arranged by size:
5013 858 728
EaeI y'GGCC_r
Cuts at: 1054 2204 3314 3515 4184 4358 4749 4776 6121
Size: 1150 1110 201 669 174 391 27 1345
Cuts at: 6121 1054
Size: 1532
Fragments arranged by size:
1532 1345 1150 1110 669 391 201 174
27
EagI C'GGCC_G
Cuts at: 1054 4184 1054
Size: 3130 3469
EarI CTCTTCn'nnn_
Cuts at: 692 1375 1418 1504 2654 4622 4832 692
Size: 683 43 86 1150 1968 210 2459
Fragments arranged by size:
2459 1968 1150 683 210 86 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2866 4807 5117 5263 6409 2866
Size: 1941 310 146 1146 3056
Fragments arranged by size:
3056 1941 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3611 3611
Size: 6599
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 752 1103 1477 1676 1825 2467 2672 2806
Size: 481 351 374 199 149 642 205 134
Cuts at: 2806 2926 2953 3214 3264 3362 3560 4424 4856
Size: 120 27 261 50 98 198 864 432
Cuts at: 4856 5603 5871 271
Size: 747 268 999
Fragments arranged by size:
999 864 747 642 481 432 374 351
268 261 205 199 198 149 134 120
98 50 27
EcoNI CCTnn'n_nnAGG
Cuts at: 952 1033 1132 1301 952
Size: 81 99 169 6250
Fragments arranged by size:
6250 169 99 81
EcoO109I rG'GnC_Cy
Cuts at: 943 1809 3286 3738 943
Size: 866 1477 452 3804
Fragments arranged by size:
3804 1477 866 452
EcoRI G'AATT_C
Cuts at: 1883 3603 1883
Size: 1720 4879
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2003 3579 4618 2003
Size: 1576 1039 3984
Fragments arranged by size:
3984 1576 1039
FspI TGC'GCA
Cuts at: 4380 4380
Size: 6599
HaeII r_GCGC'y
Cuts at: 4033 4281 5303 6282 4033
Size: 248 1022 979 4350
Fragments arranged by size:
4350 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 438 470 628 660 1503 1535 2494 2526 2779
Size: 32 158 32 843 32 959 32 253
Cuts at: 2779 2811 3075 3107 3533 3565 4441 4471 4473
Size: 32 264 32 426 32 876 30 2
Cuts at: 4473 4503 438
Size: 30 2534
Fragments arranged by size:
2534 959 876 843 426 264 253 158
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1741 2502 3638 1741
Size: 761 1136 4702
Fragments arranged by size:
4702 1136 761
HindIII A'AGCT_T
Cuts at: 2644 3398 2644
Size: 754 5845
KasI G'GCGC_C
Cuts at: 4029 4277 4029
Size: 248 6351
KpnI G_GTAC'C
Cuts at: 658 1607 3619 658
Size: 949 2012 3638
Fragments arranged by size:
3638 2012 949
MluI A'CGCG_T
Cuts at: 6032 6032
Size: 6599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 488 5270 5454 488
Size: 4782 184 1633
Fragments arranged by size:
4782 1633 184
MscI TGG'CCA
Cuts at: 2206 3517 4360 2206
Size: 1311 843 4445
Fragments arranged by size:
4445 1311 843
MslI CAynn'nnrTG
Cuts at: 1703 2362 4588 4715 1703
Size: 659 2226 127 3587
Fragments arranged by size:
3587 2226 659 127
MspA1I CmG'CkG
Cuts at: 1093 2580 3710 4024 4384 5397 5642 1093
Size: 1487 1130 314 360 1013 245 2050
Fragments arranged by size:
2050 1487 1130 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4780 4780
Size: 6599
NarI GG'CG_CC
Cuts at: 4030 4278 4030
Size: 248 6351
NcoI C'CATG_G
Cuts at: 107 385 2031 2130 107
Size: 278 1646 99 4576
Fragments arranged by size:
4576 1646 278 99
NdeI CA'TA_TG
Cuts at: 2172 2172
Size: 6599
NgoMIV G'CCGG_C
Cuts at: 4778 4778
Size: 6599
NsiI A_TGCA'T
Cuts at: 343 441 343
Size: 98 6501
NspI r_CATG'y
Cuts at: 439 3069 3171 3372 3652 4152 5059 439
Size: 2630 102 201 280 500 907 1979
Fragments arranged by size:
2630 1979 907 500 280 201 102
PasI CC'CwG_GG
Cuts at: 1128 1128
Size: 6599
PciI A'CATG_T
Cuts at: 5055 5055
Size: 6599
PflMI CCAn_nnn'nTGG
Cuts at: 1455 1614 1455
Size: 159 6440
PfoI T'CCnGG_A
Cuts at: 1080 1448 1080
Size: 368 6231
PmeI GTTT'AAAC
Cuts at: 3598 3598
Size: 6599
PmlI CAC'GTG
Cuts at: 215 2152 215
Size: 1937 4662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 500 532 5738 5770 500
Size: 32 5206 32 1329
Fragments arranged by size:
5206 1329 32 32
PpuMI rG'GwC_Cy
Cuts at: 943 1809 943
Size: 866 5733
PshAI GACnn'nnGTC
Cuts at: 160 1424 160
Size: 1264 5335
PstI C_TGCA'G
Cuts at: 3646 4331 3646
Size: 685 5914
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6599
PvuII CAG'CTG
Cuts at: 2580 4024 4384 2580
Size: 1444 360 4795
Fragments arranged by size:
4795 1444 360
RsrII CG'GwC_CG
Cuts at: 4794 4794
Size: 6599
SacI G_AGCT'C
Cuts at: 3613 3613
Size: 6599
SalI G'TCGA_C
Cuts at: 3636 3636
Size: 6599
SanDI GG'GwC_CC
Cuts at: 943 943
Size: 6599
SapI GCTCTTCn'nnn_
Cuts at: 1375 1418 4622 4832 1375
Size: 43 3204 210 3142
Fragments arranged by size:
3204 3142 210 43
SbfI CC_TGCA'GG
Cuts at: 3646 3646
Size: 6599
ScaI AGT'ACT
Cuts at: 1466 6116 1466
Size: 4650 1949
SexAI A'CCwGG_T
Cuts at: 1571 1571
Size: 6599
SfcI C'TryA_G
Cuts at: 33 674 2193 3642 4327 5320 5511 33
Size: 641 1519 1449 685 993 191 1121
Fragments arranged by size:
1519 1449 1121 993 685 641 191
SfoI GGC'GCC
Cuts at: 4031 4279 4031
Size: 248 6351
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6599
SmaI CCC'GGG
Cuts at: 3621 3621
Size: 6599
SmlI C'TyrA_G
Cuts at: 151 1756 3754 4669 5161 5423 151
Size: 1605 1998 915 492 262 1327
Fragments arranged by size:
1998 1605 1327 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6599
SphI G_CATG'C
Cuts at: 439 3069 3652 4152 439
Size: 2630 583 500 2886
Fragments arranged by size:
2886 2630 583 500
StyI C'CwwG_G
Cuts at: 107 385 780 2031 2130 2207 3563 3733 4087
Size: 278 395 1251 99 77 1356 170 354
Cuts at: 4087 107
Size: 2619
Fragments arranged by size:
2619 1356 1251 395 354 278 170 99
77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1033 1120 1726 3422 4182 4315 4774 4983 1033
Size: 87 606 1696 760 133 459 209 2649
Fragments arranged by size:
2649 1696 760 606 459 209 133 87
TatI w'GTAC_w
Cuts at: 959 1464 6114 959
Size: 505 4650 1444
Fragments arranged by size:
4650 1444 505
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 225 257 1548 1580 2842 2874 6116 6148 225
Size: 32 1291 32 1262 32 3242 32 676
Fragments arranged by size:
3242 1291 1262 676 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2985 4396 2985
Size: 1411 5188
XbaI T'CTAG_A
Cuts at: 1623 3630 1623
Size: 2007 4592
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1651 3249 3561 4094 1651
Size: 1598 312 533 4156
Fragments arranged by size:
4156 1598 533 312
XmaI C'CCGG_G
Cuts at: 3619 3619
Size: 6599
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BcgI BmtI BsiWI BsmI BsrGI
BssHII BstZ17I CspCI EcoRV FseI FspAI HpaI MfeI
NheI NotI NruI PacI PsiI PspOMI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI