Restriction map for
pF1KE2756
(Circular) (Six-base) MAPSORT of: pF1KE2756.seq Check: 5784 from: 1 to: 4580
pF1KE2756 4580 bp
With 182 enzymes: SgfI *
July 18, 2019 20:57 ..
AccI GT'mk_AC
Cuts at: 1618 1618
Size: 4580
Acc65I G'GTAC_C
Cuts at: 1596 1596
Size: 4580
AclI AA'CG_TT
Cuts at: 4420 4420
Size: 4580
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 114 218 359 854 1022 2405 2837 3584 3852
Size: 104 141 495 168 1383 432 747 268
Cuts at: 3852 114
Size: 842
Fragments arranged by size:
1383 842 747 495 432 268 168 141
104
AfeI AGC'GCT
Cuts at: 239 239
Size: 4580
AflIII A'CryG_T
Cuts at: 1932 3036 4013 1932
Size: 1104 977 2499
Fragments arranged by size:
2499 1104 977
AgeI A'CCGG_T
Cuts at: 2968 2968
Size: 4580
AhdI GACnn_n'nnGTC
Cuts at: 422 422
Size: 4580
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1550 1582 1550
Size: 32 4548
AlwNI CAG_nnn'CTG
Cuts at: 1885 3452 1885
Size: 1567 3013
ApaLI G'TGCA_C
Cuts at: 3350 3350
Size: 4580
ApoI r'AATT_y
Cuts at: 369 1584 3933 4189 369
Size: 1215 2349 256 760
Fragments arranged by size:
2349 1215 760 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4580
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4580
AvaI C'yCGr_G
Cuts at: 1600 1600
Size: 4580
BamHI G'GATC_C
Cuts at: 1605 1605
Size: 4580
BanI G'GyrC_C
Cuts at: 502 1596 2010 2258 2293 502
Size: 1094 414 248 35 2789
Fragments arranged by size:
2789 1094 414 248 35
BanII G_rGCy'C
Cuts at: 256 680 731 854 1594 2624 256
Size: 424 51 123 740 1030 2212
Fragments arranged by size:
2212 1030 740 424 123 51
BbeI G_GCGC'C
Cuts at: 2014 2262 2014
Size: 248 4332
BbsI GAAGACnn'nnnn_
Cuts at: 233 906 1388 1535 233
Size: 673 482 147 3278
Fragments arranged by size:
3278 673 482 147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 675 709 675
Size: 34 4546
BciVI GTATCCnnnnn_n'
Cuts at: 2478 3239 4580 2478
Size: 761 1341 2478
Fragments arranged by size:
2478 1341 761
BclI T'GATC_A
Cuts at: 288 2100 288
Size: 1812 2768
BglI GCCn_nnn'nGGC
Cuts at: 457 457
Size: 4580
BglII A'GATC_T
Cuts at: 2095 4132 2095
Size: 2037 2543
BlpI GC'TnA_GC
Cuts at: 241 848 1692 241
Size: 607 844 3129
Fragments arranged by size:
3129 844 607
Bme1580I G_kGCm'C
Cuts at: 505 2205 2298 3354 505
Size: 1700 93 1056 1731
Fragments arranged by size:
1731 1700 1056 93
BmgBI CAC'GTC
Cuts at: 1196 1196
Size: 4580
BmrI ACTGGGnnnn_n'
Cuts at: 645 1792 1873 2208 4352 645
Size: 1147 81 335 2144 873
Fragments arranged by size:
2144 1147 873 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1170 2947 1170
Size: 1777 2803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 895 1165 832
Size: 63 270 4247
Fragments arranged by size:
4247 270 63
Bpu10I CC'TnA_GC
Cuts at: 537 537
Size: 4580
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1756 2635 3127 3425 1756
Size: 879 492 298 2911
Fragments arranged by size:
2911 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 658 4284 658
Size: 3626 954
BsaAI yAC'GTr
Cuts at: 1933 1933
Size: 4580
BsaBI GATnn'nnATC
Cuts at: 6 287 2094 4131 6
Size: 281 1807 2037 455
Fragments arranged by size:
2037 1807 455 281
BsaHI Gr'CG_yC
Cuts at: 1366 2011 2259 2961 4457 1366
Size: 645 248 702 1496 1489
Fragments arranged by size:
1496 1489 702 645 248
BsaWI w'CCGG_w
Cuts at: 524 1769 2290 2968 3242 3389 3800 524
Size: 1245 521 678 274 147 411 1304
Fragments arranged by size:
1304 1245 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 321 351 1550 1580 321
Size: 30 1199 30 3321
Fragments arranged by size:
3321 1199 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 289 829 949 1372 289
Size: 540 120 423 3497
Fragments arranged by size:
3497 540 423 120
BseYI C'CCAG_C
Cuts at: 2005 2763 3340 3836 2005
Size: 758 577 496 2749
Fragments arranged by size:
2749 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 566
Size: 4580
BsiEI CG_ry'CG
Cuts at: 104 461 2122 2168 3376 104
Size: 357 1661 46 1208 1308
Fragments arranged by size:
1661 1308 1208 357 46
BsiHKAI G_wGCw'C
Cuts at: 256 629 680 731 1475 1594 2372 2562 3354
Size: 373 51 51 744 119 778 190 792
Cuts at: 3354 256
Size: 1482
Fragments arranged by size:
1482 792 778 744 373 190 119 51
51
Bsp1286I G_dGCh'C
Cuts at: 256 505 629 680 731 854 1475 1594 2205
Size: 249 124 51 51 123 621 119 611
Cuts at: 2205 2298 2372 2562 2624 3354 256
Size: 93 74 190 62 730 1482
Fragments arranged by size:
1482 730 621 611 249 190 124 123
119 93 74 62 51 51
BspEI T'CCGG_A
Cuts at: 3800 3800
Size: 4580
BspHI T'CATG_A
Cuts at: 413 992 3756 413
Size: 579 2764 1237
Fragments arranged by size:
2764 1237 579
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1630 1844 2146 2527 1630
Size: 214 302 381 3683
Fragments arranged by size:
3683 381 302 214
BsrBI CCG'CTC
Cuts at: 50 744 2872 2926 4410 4575 50
Size: 694 2128 54 1484 165 55
Fragments arranged by size:
2128 1484 694 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1059 2492 1059
Size: 1433 3147
BsrFI r'CCGG_y
Cuts at: 2578 2759 2968 2578
Size: 181 209 4190
Fragments arranged by size:
4190 209 181
BssSI C'ACGA_G
Cuts at: 2851 3209 2851
Size: 358 4222
BstAPI GCAn_nnn'nTGC
Cuts at: 1957 1957
Size: 4580
BstBI TT'CG_AA
Cuts at: 2941 2941
Size: 4580
BstXI CCAn_nnnn'nTGG
Cuts at: 571 571
Size: 4580
BstYI r'GATC_y
Cuts at: 1 1605 2072 2095 2430 2676 3688 3774 3786
Size: 1604 467 23 335 246 1012 86 12
Cuts at: 3786 4132 1
Size: 346 449
Fragments arranged by size:
1604 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2530 2711 2874 2530
Size: 181 163 4236
Fragments arranged by size:
4236 181 163
BtsI GCAGTG_nn'
Cuts at: 453 1176 1823 453
Size: 723 647 3210
Fragments arranged by size:
3210 723 647
DraI TTT'AAA
Cuts at: 1579 1579
Size: 4580
DraIII CAC_nnn'GTG
Cuts at: 639 1312 639
Size: 673 3907
DrdI GACnn_nn'nnGTC
Cuts at: 1390 2286 3144 1390
Size: 896 858 2826
Fragments arranged by size:
2826 896 858
EaeI y'GGCC_r
Cuts at: 116 458 1097 2165 2339 2730 2757 4102 116
Size: 342 639 1068 174 391 27 1345 594
Fragments arranged by size:
1345 1068 639 594 391 342 174 27
EagI C'GGCC_G
Cuts at: 458 2165 458
Size: 1707 2873
EarI CTCTTCn'nnn_
Cuts at: 2603 2813 2603
Size: 210 4370
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 619 2788 3098 3244 4390 619
Size: 2169 310 146 1146 809
Fragments arranged by size:
2169 1146 809 310 146
EcoICRI GAG'CTC
Cuts at: 254 678 729 1592 254
Size: 424 51 863 3242
Fragments arranged by size:
3242 863 424 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 114 218 359 832 854 895 1022 1165 2405
Size: 104 141 473 22 41 127 143 1240
Cuts at: 2405 2837 3584 3852 114
Size: 432 747 268 842
Fragments arranged by size:
1240 842 747 473 432 268 143 141
127 104 41 22
EcoNI CCTnn'n_nnAGG
Cuts at: 736 736
Size: 4580
EcoO109I rG'GnC_Cy
Cuts at: 786 980 1719 786
Size: 194 739 3647
Fragments arranged by size:
3647 739 194
EcoRI G'AATT_C
Cuts at: 1584 1584
Size: 4580
EcoRV GAT'ATC
Cuts at: 1418 1418
Size: 4580
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1001 2599 1001
Size: 1598 2982
FspI TGC'GCA
Cuts at: 2361 2361
Size: 4580
HaeII r_GCGC'y
Cuts at: 241 1082 2014 2262 3284 4263 241
Size: 841 932 248 1022 979 558
Fragments arranged by size:
1022 979 932 841 558 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 408 440 2422 2452 2454 2484 408
Size: 32 1982 30 2 30 2504
Fragments arranged by size:
2504 1982 32 30 30 2
HincII GTy'rAC
Cuts at: 1395 1619 1395
Size: 224 4356
HindIII A'AGCT_T
Cuts at: 1223 1246 1223
Size: 23 4557
KasI G'GCGC_C
Cuts at: 2010 2258 2010
Size: 248 4332
KpnI G_GTAC'C
Cuts at: 1600 1600
Size: 4580
MluI A'CGCG_T
Cuts at: 4013 4013
Size: 4580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 364 3251 3435 364
Size: 2887 184 1509
Fragments arranged by size:
2887 1509 184
MscI TGG'CCA
Cuts at: 2341 2341
Size: 4580
MslI CAynn'nnrTG
Cuts at: 826 1013 2569 2696 826
Size: 187 1556 127 2710
Fragments arranged by size:
2710 1556 187 127
MspA1I CmG'CkG
Cuts at: 891 1118 1691 2005 2365 3378 3623 891
Size: 227 573 314 360 1013 245 1848
Fragments arranged by size:
1848 1013 573 360 314 245 227
NaeI GCC'GGC
Cuts at: 2761 2761
Size: 4580
NarI GG'CG_CC
Cuts at: 2011 2259 2011
Size: 248 4332
NdeI CA'TA_TG
Cuts at: 1111 1111
Size: 4580
NgoMIV G'CCGG_C
Cuts at: 2759 2759
Size: 4580
NspI r_CATG'y
Cuts at: 1633 2133 3040 1633
Size: 500 907 3173
Fragments arranged by size:
3173 907 500
PciI A'CATG_T
Cuts at: 3036 3036
Size: 4580
PfoI T'CCnGG_A
Cuts at: 701 701
Size: 4580
PmeI GTTT'AAAC
Cuts at: 1579 1579
Size: 4580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1550 1582 3719 3751 1550
Size: 32 2137 32 2379
Fragments arranged by size:
2379 2137 32 32
PpuMI rG'GwC_Cy
Cuts at: 786 980 786
Size: 194 4386
PstI C_TGCA'G
Cuts at: 756 1627 2312 756
Size: 871 685 3024
Fragments arranged by size:
3024 871 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4580
PvuII CAG'CTG
Cuts at: 891 2005 2365 891
Size: 1114 360 3106
Fragments arranged by size:
3106 1114 360
RsrII CG'GwC_CG
Cuts at: 2775 2775
Size: 4580
SacI G_AGCT'C
Cuts at: 256 680 731 1594 256
Size: 424 51 863 3242
Fragments arranged by size:
3242 863 424 51
SalI G'TCGA_C
Cuts at: 1617 1617
Size: 4580
SapI GCTCTTCn'nnn_
Cuts at: 2603 2813 2603
Size: 210 4370
SbfI CC_TGCA'GG
Cuts at: 1627 1627
Size: 4580
ScaI AGT'ACT
Cuts at: 4097 4097
Size: 4580
SfcI C'TryA_G
Cuts at: 33 752 1623 2308 3301 3492 33
Size: 719 871 685 993 191 1121
Fragments arranged by size:
1121 993 871 719 685 191
SfoI GGC'GCC
Cuts at: 2012 2260 2012
Size: 248 4332
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4580
SmaI CCC'GGG
Cuts at: 1602 1602
Size: 4580
SmlI C'TyrA_G
Cuts at: 1735 2650 3142 3404 1735
Size: 915 492 262 2911
Fragments arranged by size:
2911 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4580
SphI G_CATG'C
Cuts at: 1633 2133 1633
Size: 500 4080
SspI AAT'ATT
Cuts at: 1495 1495
Size: 4580
StyI C'CwwG_G
Cuts at: 1714 2068 1714
Size: 354 4226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 363 627 1083 2163 2296 2755 2964 363
Size: 264 456 1080 133 459 209 1979
Fragments arranged by size:
1979 1080 459 456 264 209 133
TatI w'GTAC_w
Cuts at: 110 4095 110
Size: 3985 595
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 625 657 1298 1330 4097 4129 625
Size: 32 641 32 2767 32 1076
Fragments arranged by size:
2767 1076 641 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2377 2377
Size: 4580
XbaI T'CTAG_A
Cuts at: 1611 1611
Size: 4580
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 304 2075 304
Size: 1771 2809
XmaI C'CCGG_G
Cuts at: 1600 1600
Size: 4580
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI ApaI AscI AvrII
BaeI BbvCI BfrBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI FseI
FspAI HpaI MfeI NcoI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI