(Circular) (Six-base) MAPSORT of: pF1KE2758.seq Check: 521 from: 1 to: 5075 pF1KE2758 5075 bp With 182 enzymes: SgfI * August 1, 2019 16:47 .. AccI GT'mk_AC Cuts at: 2113 2113 Size: 5075 Acc65I G'GTAC_C Cuts at: 2091 2091 Size: 5075 AclI AA'CG_TT Cuts at: 4915 4915 Size: 5075 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 136 437 2900 3332 4079 4347 136 Size: 301 2463 432 747 268 864 Fragments arranged by size: 2463 864 747 432 301 268 AflIII A'CryG_T Cuts at: 2427 3531 4508 2427 Size: 1104 977 2994 Fragments arranged by size: 2994 1104 977 AgeI A'CCGG_T Cuts at: 3463 3463 Size: 5075 AlwNI CAG_nnn'CTG Cuts at: 2380 3947 2380 Size: 1567 3508 ApaI G_GGCC'C Cuts at: 324 550 1859 324 Size: 226 1309 3540 Fragments arranged by size: 3540 1309 226 ApaLI G'TGCA_C Cuts at: 3845 3845 Size: 5075 ApoI r'AATT_y Cuts at: 863 2079 4428 4684 863 Size: 1216 2349 256 1254 Fragments arranged by size: 2349 1254 1216 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5075 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5075 AvaI C'yCGr_G Cuts at: 895 2095 895 Size: 1200 3875 AvrII C'CTAG_G Cuts at: 595 595 Size: 5075 BamHI G'GATC_C Cuts at: 2100 2100 Size: 5075 BanI G'GyrC_C Cuts at: 2091 2505 2753 2788 2091 Size: 414 248 35 4378 Fragments arranged by size: 4378 414 248 35 BanII G_rGCy'C Cuts at: 152 324 550 896 1248 1338 1859 2089 3119 Size: 172 226 346 352 90 521 230 1030 Cuts at: 3119 152 Size: 2108 Fragments arranged by size: 2108 1030 521 352 346 230 226 172 90 BbeI G_GCGC'C Cuts at: 2509 2757 2509 Size: 248 4827 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 5041 BciVI GTATCCnnnnn_n' Cuts at: 2973 3734 5075 2973 Size: 761 1341 2973 Fragments arranged by size: 2973 1341 761 BclI T'GATC_A Cuts at: 585 639 2595 585 Size: 54 1956 3065 Fragments arranged by size: 3065 1956 54 BglI GCCn_nnn'nGGC Cuts at: 217 319 473 716 1700 1727 1863 1997 217 Size: 102 154 243 984 27 136 134 3295 Fragments arranged by size: 3295 984 243 154 136 134 102 27 BglII A'GATC_T Cuts at: 2590 4627 2590 Size: 2037 3038 BlpI GC'TnA_GC Cuts at: 2187 2187 Size: 5075 Bme1580I G_kGCm'C Cuts at: 192 324 550 1859 2700 2793 3849 192 Size: 132 226 1309 841 93 1056 1418 Fragments arranged by size: 1418 1309 1056 841 226 132 93 BmrI ACTGGGnnnn_n' Cuts at: 2287 2368 2703 4847 2287 Size: 81 335 2144 2515 Fragments arranged by size: 2515 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3442 3442 Size: 5075 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 268 464 680 698 926 971 998 1262 1352 Size: 196 216 18 228 45 27 264 90 Cuts at: 1352 1496 268 Size: 144 3847 Fragments arranged by size: 3847 264 228 216 196 144 90 45 27 18 Bpu10I CC'TnA_GC Cuts at: 1337 1337 Size: 5075 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 346 1136 1460 2251 3130 3622 3920 346 Size: 790 324 791 879 492 298 1501 Fragments arranged by size: 1501 879 791 790 492 324 298 BsaI GGTCTCn'nnnn_ Cuts at: 4779 4779 Size: 5075 BsaAI yAC'GTr Cuts at: 2428 2428 Size: 5075 BsaBI GATnn'nnATC Cuts at: 6 2589 4626 6 Size: 2583 2037 455 Fragments arranged by size: 2583 2037 455 BsaHI Gr'CG_yC Cuts at: 2506 2754 3456 4952 2506 Size: 248 702 1496 2629 Fragments arranged by size: 2629 1496 702 248 BsaWI w'CCGG_w Cuts at: 2264 2785 3463 3737 3884 4295 2264 Size: 521 678 274 147 411 3044 Fragments arranged by size: 3044 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 524 524 Size: 5075 BseYI C'CCAG_C Cuts at: 468 2500 3258 3835 4331 468 Size: 2032 758 577 496 1212 Fragments arranged by size: 2032 1212 758 577 496 BsiEI CG_ry'CG Cuts at: 104 2617 2663 3871 104 Size: 2513 46 1208 1308 Fragments arranged by size: 2513 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 858 1338 2089 2867 3057 3849 858 Size: 480 751 778 190 792 2084 Fragments arranged by size: 2084 792 778 751 480 190 BsmI GAATG_Cn' Cuts at: 770 1667 770 Size: 897 4178 BsmBI CGTCTCn'nnnn_ Cuts at: 965 965 Size: 5075 Bsp1286I G_dGCh'C Cuts at: 152 192 324 550 858 896 1248 1338 1859 Size: 40 132 226 308 38 352 90 521 Cuts at: 1859 2089 2700 2793 2867 3057 3119 3849 152 Size: 230 611 93 74 190 62 730 1378 Fragments arranged by size: 1378 730 611 521 352 308 230 226 190 132 93 90 74 62 40 38 BspEI T'CCGG_A Cuts at: 4295 4295 Size: 5075 BspHI T'CATG_A Cuts at: 4251 4251 Size: 5075 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1789 2125 2339 2641 3022 1789 Size: 336 214 302 381 3842 Fragments arranged by size: 3842 381 336 302 214 BsrBI CCG'CTC Cuts at: 50 301 3367 3421 4905 5070 50 Size: 251 3066 54 1484 165 55 Fragments arranged by size: 3066 1484 251 165 55 54 BsrDI GCAATG_nn' Cuts at: 2987 2987 Size: 5075 BsrFI r'CCGG_y Cuts at: 3073 3254 3463 3073 Size: 181 209 4685 Fragments arranged by size: 4685 209 181 BssSI C'ACGA_G Cuts at: 3346 3704 3346 Size: 358 4717 BstAPI GCAn_nnn'nTGC Cuts at: 2452 2452 Size: 5075 BstBI TT'CG_AA Cuts at: 3436 3436 Size: 5075 BstEII G'GTnAC_C Cuts at: 673 1015 673 Size: 342 4733 BstYI r'GATC_y Cuts at: 1 154 1507 2100 2567 2590 2925 3171 4183 Size: 153 1353 593 467 23 335 246 1012 Cuts at: 4183 4269 4281 4627 1 Size: 86 12 346 449 Fragments arranged by size: 1353 1012 593 467 449 346 335 246 153 86 23 12 Bsu36I CC'TnA_GG Cuts at: 542 938 542 Size: 396 4679 BtgI C'CryG_G Cuts at: 242 759 1716 1733 1788 1912 1959 242 Size: 517 957 17 55 124 47 3358 Fragments arranged by size: 3358 957 517 124 55 47 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3025 3206 3369 3025 Size: 181 163 4731 Fragments arranged by size: 4731 181 163 BtsI GCAGTG_nn' Cuts at: 2318 2318 Size: 5075 DraI TTT'AAA Cuts at: 2074 2074 Size: 5075 DraIII CAC_nnn'GTG Cuts at: 1918 1918 Size: 5075 DrdI GACnn_nn'nnGTC Cuts at: 536 1349 2781 3639 536 Size: 813 1432 858 1972 Fragments arranged by size: 1972 1432 858 813 EaeI y'GGCC_r Cuts at: 239 2660 2834 3225 3252 4597 239 Size: 2421 174 391 27 1345 717 Fragments arranged by size: 2421 1345 717 391 174 27 EagI C'GGCC_G Cuts at: 2660 2660 Size: 5075 EarI CTCTTCn'nnn_ Cuts at: 501 1215 3098 3308 501 Size: 714 1883 210 2268 Fragments arranged by size: 2268 1883 714 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 300 3283 3593 3739 4885 300 Size: 2983 310 146 1146 490 Fragments arranged by size: 2983 1146 490 310 146 EcoICRI GAG'CTC Cuts at: 1336 2087 1336 Size: 751 4324 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 136 268 437 464 680 698 926 971 998 Size: 132 169 27 216 18 228 45 27 Cuts at: 998 1262 1352 1496 2900 3332 4079 4347 136 Size: 264 90 144 1404 432 747 268 864 Fragments arranged by size: 1404 864 747 432 268 264 228 216 169 144 132 90 45 27 27 18 EcoNI CCTnn'n_nnAGG Cuts at: 667 1220 1425 667 Size: 553 205 4317 Fragments arranged by size: 4317 553 205 EcoO109I rG'GnC_Cy Cuts at: 162 320 321 475 546 547 592 691 700 Size: 158 1 154 71 1 45 99 9 Cuts at: 700 817 826 934 1210 1453 1572 1678 1711 Size: 117 9 108 276 243 119 106 33 Cuts at: 1711 1738 1783 1801 1855 1856 2214 162 Size: 27 45 18 54 1 358 3023 Fragments arranged by size: 3023 358 276 243 158 154 119 117 108 106 99 71 54 45 45 33 27 18 9 9 1 1 1 EcoRI G'AATT_C Cuts at: 863 2079 863 Size: 1216 3859 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3094 3094 Size: 5075 FspI TGC'GCA Cuts at: 2856 2856 Size: 5075 HaeII r_GCGC'y Cuts at: 2509 2757 3779 4758 2509 Size: 248 1022 979 2826 Fragments arranged by size: 2826 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 139 171 285 317 804 836 1009 1041 1492 Size: 32 114 32 487 32 173 32 451 Cuts at: 1492 1524 2917 2947 2949 2979 139 Size: 32 1393 30 2 30 2235 Fragments arranged by size: 2235 1393 487 451 173 114 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 2114 2114 Size: 5075 KasI G'GCGC_C Cuts at: 2505 2753 2505 Size: 248 4827 KpnI G_GTAC'C Cuts at: 2095 2095 Size: 5075 MfeI C'AATT_G Cuts at: 2026 2026 Size: 5075 MluI A'CGCG_T Cuts at: 4508 4508 Size: 5075 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3746 3930 3746 Size: 184 4891 MscI TGG'CCA Cuts at: 2836 2836 Size: 5075 MslI CAynn'nnrTG Cuts at: 3064 3191 3064 Size: 127 4948 MspA1I CmG'CkG Cuts at: 2186 2500 2860 3873 4118 2186 Size: 314 360 1013 245 3143 Fragments arranged by size: 3143 1013 360 314 245 NaeI GCC'GGC Cuts at: 3256 3256 Size: 5075 NarI GG'CG_CC Cuts at: 2506 2754 2506 Size: 248 4827 NcoI C'CATG_G Cuts at: 1716 1733 1788 1716 Size: 17 55 5003 Fragments arranged by size: 5003 55 17 NdeI CA'TA_TG Cuts at: 419 419 Size: 5075 NgoMIV G'CCGG_C Cuts at: 3254 3254 Size: 5075 NspI r_CATG'y Cuts at: 2128 2628 3535 2128 Size: 500 907 3668 Fragments arranged by size: 3668 907 500 PciI A'CATG_T Cuts at: 3531 3531 Size: 5075 PfoI T'CCnGG_A Cuts at: 525 696 831 1107 1215 1467 1512 1611 1752 Size: 171 135 276 108 252 45 99 141 Cuts at: 1752 525 Size: 3848 Fragments arranged by size: 3848 276 252 171 141 135 108 99 45 PmeI GTTT'AAAC Cuts at: 2074 2074 Size: 5075 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4214 4246 4214 Size: 32 5043 PpuMI rG'GwC_Cy Cuts at: 162 592 700 817 934 1453 1572 1678 1711 Size: 430 108 117 117 519 119 106 33 Cuts at: 1711 1738 1783 1801 162 Size: 27 45 18 3436 Fragments arranged by size: 3436 519 430 119 117 117 108 106 45 33 27 18 PspOMI G'GGCC_C Cuts at: 320 546 1855 320 Size: 226 1309 3540 Fragments arranged by size: 3540 1309 226 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 1151 1183 1151 Size: 32 5043 PstI C_TGCA'G Cuts at: 1038 1800 2122 2807 1038 Size: 762 322 685 3306 Fragments arranged by size: 3306 762 685 322 PvuI CG_AT'CG Cuts at: 104 104 Size: 5075 PvuII CAG'CTG Cuts at: 2500 2860 2500 Size: 360 4715 RsrII CG'GwC_CG Cuts at: 3270 3270 Size: 5075 SacI G_AGCT'C Cuts at: 1338 2089 1338 Size: 751 4324 SalI G'TCGA_C Cuts at: 2112 2112 Size: 5075 SanDI GG'GwC_CC Cuts at: 1453 1572 1783 1453 Size: 119 211 4745 Fragments arranged by size: 4745 211 119 SapI GCTCTTCn'nnn_ Cuts at: 3098 3308 3098 Size: 210 4865 SbfI CC_TGCA'GG Cuts at: 1800 2122 1800 Size: 322 4753 ScaI AGT'ACT Cuts at: 4592 4592 Size: 5075 SexAI A'CCwGG_T Cuts at: 1134 1170 1356 1707 1134 Size: 36 186 351 4502 Fragments arranged by size: 4502 351 186 36 SfcI C'TryA_G Cuts at: 33 1034 1796 2118 2803 3796 3987 33 Size: 1001 762 322 685 993 191 1121 Fragments arranged by size: 1121 1001 993 762 685 322 191 SfiI GGCCn_nnn'nGGCC Cuts at: 716 1700 1727 1997 716 Size: 984 27 270 3794 Fragments arranged by size: 3794 984 270 27 SfoI GGC'GCC Cuts at: 2507 2755 2507 Size: 248 4827 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5075 SmaI CCC'GGG Cuts at: 897 2097 897 Size: 1200 3875 SmlI C'TyrA_G Cuts at: 361 1151 1475 2230 3145 3637 3899 361 Size: 790 324 755 915 492 262 1537 Fragments arranged by size: 1537 915 790 755 492 324 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5075 SphI G_CATG'C Cuts at: 2128 2628 2128 Size: 500 4575 StyI C'CwwG_G Cuts at: 595 678 992 1345 1716 1733 1788 2209 2563 Size: 83 314 353 371 17 55 421 354 Cuts at: 2563 595 Size: 3107 Fragments arranged by size: 3107 421 371 354 353 314 83 55 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 253 2658 2791 3250 3459 253 Size: 2405 133 459 209 1869 Fragments arranged by size: 2405 1869 459 209 133 TatI w'GTAC_w Cuts at: 4590 4590 Size: 5075 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1254 1286 4592 4624 1254 Size: 32 3306 32 1705 Fragments arranged by size: 3306 1705 32 32 Tth111I GACn'n_nGTC Cuts at: 2872 2872 Size: 5075 XbaI T'CTAG_A Cuts at: 2106 2106 Size: 5075 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1723 2570 1723 Size: 847 4228 XmaI C'CCGG_G Cuts at: 895 2095 895 Size: 1200 3875 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI BaeI BbsI BbvCI BfrBI BmgBI BmtI BsaXI BsgI BsiWI BsrGI BssHII BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI NheI NotI NruI NsiI PacI PasI PflMI PmlI PshAI PsiI PspXI SacII SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI