(Circular) (Six-base) MAPSORT of: pF1KE2761.seq Check: 7099 from: 1 to: 5618 pF1KE2761 5618 bp With 182 enzymes: SgfI * October 3, 2019 17:18 .. AccI GT'mk_AC Cuts at: 1215 1452 1839 2656 1215 Size: 237 387 817 4177 Fragments arranged by size: 4177 817 387 237 Acc65I G'GTAC_C Cuts at: 652 2634 652 Size: 1982 3636 AclI AA'CG_TT Cuts at: 5458 5458 Size: 5618 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1805 3443 3875 4622 4890 1805 Size: 1638 432 747 268 2533 Fragments arranged by size: 2533 1638 747 432 268 AflIII A'CryG_T Cuts at: 212 2970 4074 5051 212 Size: 2758 1104 977 779 Fragments arranged by size: 2758 1104 977 779 AgeI A'CCGG_T Cuts at: 4006 4006 Size: 5618 AhdI GACnn_n'nnGTC Cuts at: 2533 2533 Size: 5618 AlwNI CAG_nnn'CTG Cuts at: 923 2923 4490 923 Size: 2000 1567 2051 Fragments arranged by size: 2051 2000 1567 ApaLI G'TGCA_C Cuts at: 4388 4388 Size: 5618 ApoI r'AATT_y Cuts at: 351 1486 2154 2622 4971 5227 351 Size: 1135 668 468 2349 256 742 Fragments arranged by size: 2349 1135 742 668 468 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5618 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5618 AvaI C'yCGr_G Cuts at: 776 824 2638 776 Size: 48 1814 3756 Fragments arranged by size: 3756 1814 48 BamHI G'GATC_C Cuts at: 2643 2643 Size: 5618 BanI G'GyrC_C Cuts at: 652 1681 2634 3048 3296 3331 652 Size: 1029 953 414 248 35 2939 Fragments arranged by size: 2939 1029 953 414 248 35 BanII G_rGCy'C Cuts at: 2167 2632 3662 2167 Size: 465 1030 4123 Fragments arranged by size: 4123 1030 465 BbeI G_GCGC'C Cuts at: 3052 3300 3052 Size: 248 5370 BbsI GAAGACnn'nnnn_ Cuts at: 1726 2611 1726 Size: 885 4733 BbvCI CC'TCA_GC Cuts at: 2387 2387 Size: 5618 BciVI GTATCCnnnnn_n' Cuts at: 3516 4277 5618 3516 Size: 761 1341 3516 Fragments arranged by size: 3516 1341 761 BclI T'GATC_A Cuts at: 1052 3138 1052 Size: 2086 3532 BfrBI ATG'CAT Cuts at: 132 317 132 Size: 185 5433 BglII A'GATC_T Cuts at: 233 3133 5170 233 Size: 2900 2037 681 Fragments arranged by size: 2900 2037 681 BlpI GC'TnA_GC Cuts at: 811 1526 2730 811 Size: 715 1204 3699 Fragments arranged by size: 3699 1204 715 Bme1580I G_kGCm'C Cuts at: 3243 3336 4392 3243 Size: 93 1056 4469 Fragments arranged by size: 4469 1056 93 BmgBI CAC'GTC Cuts at: 1219 1837 1219 Size: 618 5000 BmrI ACTGGGnnnn_n' Cuts at: 1717 2830 2911 3246 5390 1717 Size: 1113 81 335 2144 1945 Fragments arranged by size: 2144 1945 1113 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3985 3985 Size: 5618 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1618 1618 Size: 5618 Bpu10I CC'TnA_GC Cuts at: 2387 2387 Size: 5618 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 396 2794 3673 4165 4463 396 Size: 2398 879 492 298 1551 Fragments arranged by size: 2398 1551 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 5322 5322 Size: 5618 BsaAI yAC'GTr Cuts at: 1741 2971 1741 Size: 1230 4388 BsaBI GATnn'nnATC Cuts at: 6 3132 5169 6 Size: 3126 2037 455 Fragments arranged by size: 3126 2037 455 BsaHI Gr'CG_yC Cuts at: 3049 3297 3999 5495 3049 Size: 248 702 1496 3172 Fragments arranged by size: 3172 1496 702 248 BsaWI w'CCGG_w Cuts at: 2807 3328 4006 4280 4427 4838 2807 Size: 521 678 274 147 411 3587 Fragments arranged by size: 3587 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 2567 2597 2567 Size: 30 5588 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 932 1356 932 Size: 424 5194 BseYI C'CCAG_C Cuts at: 744 1536 2176 2491 3043 3801 4378 4874 744 Size: 792 640 315 552 758 577 496 1488 Fragments arranged by size: 1488 792 758 640 577 552 496 315 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 2316 2316 Size: 5618 BsiEI CG_ry'CG Cuts at: 104 3160 3206 4414 104 Size: 3056 46 1208 1308 Fragments arranged by size: 3056 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 2632 3410 3600 4392 2632 Size: 778 190 792 3858 Fragments arranged by size: 3858 792 778 190 BsmI GAATG_Cn' Cuts at: 134 1473 134 Size: 1339 4279 Bsp1286I G_dGCh'C Cuts at: 2167 2632 3243 3336 3410 3600 3662 4392 2167 Size: 465 611 93 74 190 62 730 3393 Fragments arranged by size: 3393 730 611 465 190 93 74 62 BspEI T'CCGG_A Cuts at: 4838 4838 Size: 5618 BspHI T'CATG_A Cuts at: 731 1569 4794 731 Size: 838 3225 1555 Fragments arranged by size: 3225 1555 838 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2034 2668 2882 3184 3565 2034 Size: 634 214 302 381 4087 Fragments arranged by size: 4087 634 381 302 214 BsrBI CCG'CTC Cuts at: 50 3910 3964 5448 5613 50 Size: 3860 54 1484 165 55 Fragments arranged by size: 3860 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2231 3530 2231 Size: 1299 4319 BsrFI r'CCGG_y Cuts at: 3616 3797 4006 3616 Size: 181 209 5228 Fragments arranged by size: 5228 209 181 BssSI C'ACGA_G Cuts at: 581 1715 1963 3889 4247 581 Size: 1134 248 1926 358 1952 Fragments arranged by size: 1952 1926 1134 358 248 BstAPI GCAn_nnn'nTGC Cuts at: 535 2995 535 Size: 2460 3158 BstBI TT'CG_AA Cuts at: 126 3979 126 Size: 3853 1765 BstYI r'GATC_y Cuts at: 1 233 2643 3110 3133 3468 3714 4726 4812 Size: 232 2410 467 23 335 246 1012 86 Cuts at: 4812 4824 5170 1 Size: 12 346 449 Fragments arranged by size: 2410 1012 467 449 346 335 246 232 86 23 12 BtgI C'CryG_G Cuts at: 1910 2171 1910 Size: 261 5357 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3568 3749 3912 3568 Size: 181 163 5274 Fragments arranged by size: 5274 181 163 BtsI GCAGTG_nn' Cuts at: 291 1802 2861 291 Size: 1511 1059 3048 Fragments arranged by size: 3048 1511 1059 DraI TTT'AAA Cuts at: 2617 2617 Size: 5618 DrdI GACnn_nn'nnGTC Cuts at: 3324 4182 3324 Size: 858 4760 EaeI y'GGCC_r Cuts at: 3203 3377 3768 3795 5140 3203 Size: 174 391 27 1345 3681 Fragments arranged by size: 3681 1345 391 174 27 EagI C'GGCC_G Cuts at: 3203 3203 Size: 5618 EarI CTCTTCn'nnn_ Cuts at: 263 1262 2046 3641 3851 263 Size: 999 784 1595 210 2030 Fragments arranged by size: 2030 1595 999 784 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3826 4136 4282 5428 3826 Size: 310 146 1146 4016 Fragments arranged by size: 4016 1146 310 146 EcoICRI GAG'CTC Cuts at: 2630 2630 Size: 5618 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1618 1805 3443 3875 4622 4890 1618 Size: 187 1638 432 747 268 2346 Fragments arranged by size: 2346 1638 747 432 268 187 EcoO109I rG'GnC_Cy Cuts at: 601 2757 601 Size: 2156 3462 EcoRI G'AATT_C Cuts at: 2622 2622 Size: 5618 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1134 3637 1134 Size: 2503 3115 FspI TGC'GCA Cuts at: 3399 3399 Size: 5618 HaeII r_GCGC'y Cuts at: 3052 3300 4322 5301 3052 Size: 248 1022 979 3369 Fragments arranged by size: 3369 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 873 905 918 950 1126 1158 1369 1401 1551 Size: 32 13 32 176 32 211 32 150 Cuts at: 1551 1583 2519 2551 3460 3490 3492 3522 873 Size: 32 936 32 909 30 2 30 2969 Fragments arranged by size: 2969 936 909 211 176 150 32 32 32 32 32 32 30 30 13 2 HincII GTy'rAC Cuts at: 361 2142 2657 361 Size: 1781 515 3322 Fragments arranged by size: 3322 1781 515 HindIII A'AGCT_T Cuts at: 476 1612 476 Size: 1136 4482 KasI G'GCGC_C Cuts at: 3048 3296 3048 Size: 248 5370 KpnI G_GTAC'C Cuts at: 656 2638 656 Size: 1982 3636 MfeI C'AATT_G Cuts at: 1206 1206 Size: 5618 MluI A'CGCG_T Cuts at: 5051 5051 Size: 5618 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 223 572 1529 4289 4473 223 Size: 349 957 2760 184 1368 Fragments arranged by size: 2760 1368 957 349 184 MscI TGG'CCA Cuts at: 3379 3379 Size: 5618 MslI CAynn'nnrTG Cuts at: 196 211 1574 1915 3607 3734 196 Size: 15 1363 341 1692 127 2080 Fragments arranged by size: 2080 1692 1363 341 127 15 MspA1I CmG'CkG Cuts at: 2330 2729 3043 3403 4416 4661 2330 Size: 399 314 360 1013 245 3287 Fragments arranged by size: 3287 1013 399 360 314 245 NaeI GCC'GGC Cuts at: 3799 3799 Size: 5618 NarI GG'CG_CC Cuts at: 3049 3297 3049 Size: 248 5370 NcoI C'CATG_G Cuts at: 1910 2171 1910 Size: 261 5357 NgoMIV G'CCGG_C Cuts at: 3797 3797 Size: 5618 NsiI A_TGCA'T Cuts at: 134 319 134 Size: 185 5433 NspI r_CATG'y Cuts at: 216 705 2429 2671 3171 4078 216 Size: 489 1724 242 500 907 1756 Fragments arranged by size: 1756 1724 907 500 489 242 PasI CC'CwG_GG Cuts at: 158 1424 158 Size: 1266 4352 PciI A'CATG_T Cuts at: 212 4074 212 Size: 3862 1756 PmeI GTTT'AAAC Cuts at: 2617 2617 Size: 5618 PmlI CAC'GTG Cuts at: 1741 1741 Size: 5618 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4757 4789 4757 Size: 32 5586 PpuMI rG'GwC_Cy Cuts at: 601 601 Size: 5618 PsiI TTA'TAA Cuts at: 295 1306 1658 295 Size: 1011 352 4255 Fragments arranged by size: 4255 1011 352 PstI C_TGCA'G Cuts at: 1758 2665 3350 1758 Size: 907 685 4026 Fragments arranged by size: 4026 907 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 5618 PvuII CAG'CTG Cuts at: 2330 3043 3403 2330 Size: 713 360 4545 Fragments arranged by size: 4545 713 360 RsrII CG'GwC_CG Cuts at: 3813 3813 Size: 5618 SacI G_AGCT'C Cuts at: 2632 2632 Size: 5618 SalI G'TCGA_C Cuts at: 2655 2655 Size: 5618 SapI GCTCTTCn'nnn_ Cuts at: 3641 3851 3641 Size: 210 5408 SbfI CC_TGCA'GG Cuts at: 2665 2665 Size: 5618 ScaI AGT'ACT Cuts at: 1391 5135 1391 Size: 3744 1874 SfcI C'TryA_G Cuts at: 33 1178 1453 1754 1840 2345 2661 3346 4339 Size: 1145 275 301 86 505 316 685 993 Cuts at: 4339 4530 33 Size: 191 1121 Fragments arranged by size: 1145 1121 993 685 505 316 301 275 191 86 SfoI GGC'GCC Cuts at: 3050 3298 3050 Size: 248 5370 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5618 SmaI CCC'GGG Cuts at: 2640 2640 Size: 5618 SmlI C'TyrA_G Cuts at: 411 2773 3688 4180 4442 411 Size: 2362 915 492 262 1587 Fragments arranged by size: 2362 1587 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5618 SphI G_CATG'C Cuts at: 2429 2671 3171 2429 Size: 242 500 4876 Fragments arranged by size: 4876 500 242 SspI AAT'ATT Cuts at: 576 576 Size: 5618 StyI C'CwwG_G Cuts at: 758 1910 2090 2171 2586 2752 3106 758 Size: 1152 180 81 415 166 354 3270 Fragments arranged by size: 3270 1152 415 354 180 166 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2353 3201 3334 3793 4002 2353 Size: 848 133 459 209 3969 Fragments arranged by size: 3969 848 459 209 133 TatI w'GTAC_w Cuts at: 427 952 1112 1389 2011 5133 427 Size: 525 160 277 622 3122 912 Fragments arranged by size: 3122 912 622 525 277 160 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1701 1733 5135 5167 1701 Size: 32 3402 32 2152 Fragments arranged by size: 3402 2152 32 32 Tth111I GACn'n_nGTC Cuts at: 3415 3415 Size: 5618 XbaI T'CTAG_A Cuts at: 2649 2649 Size: 5618 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3113 3113 Size: 5618 XmaI C'CCGG_G Cuts at: 2638 2638 Size: 5618 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PmeI PmlI PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AleI AlfI AloI ApaI AscI AvrII BaeI BcgI BglI BmtI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI NdeI NheI NotI NruI PacI PflMI PfoI PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI