(Circular) (Six-base) MAPSORT of: pF1KE2762.seq Check: 8866 from: 1 to: 4082 pF1KE2762 4082 bp With 182 enzymes: SgfI * October 3, 2019 17:18 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 974 974 Size: 4082 AccI GT'mk_AC Cuts at: 146 737 1120 146 Size: 591 383 3108 Fragments arranged by size: 3108 591 383 Acc65I G'GTAC_C Cuts at: 1098 1098 Size: 4082 AclI AA'CG_TT Cuts at: 3922 3922 Size: 4082 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1907 2339 3086 3354 1907 Size: 432 747 268 2635 Fragments arranged by size: 2635 747 432 268 AflIII A'CryG_T Cuts at: 544 809 1434 2538 3515 544 Size: 265 625 1104 977 1111 Fragments arranged by size: 1111 1104 977 625 265 AgeI A'CCGG_T Cuts at: 2470 2470 Size: 4082 AleI CACnn'nnGTG Cuts at: 679 679 Size: 4082 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 682 682 Size: 4082 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 544 576 544 Size: 32 4050 AlwNI CAG_nnn'CTG Cuts at: 273 1387 2954 273 Size: 1114 1567 1401 Fragments arranged by size: 1567 1401 1114 ApaI G_GGCC'C Cuts at: 1031 1031 Size: 4082 ApaLI G'TGCA_C Cuts at: 2852 2852 Size: 4082 ApoI r'AATT_y Cuts at: 1086 3435 3691 1086 Size: 2349 256 1477 Fragments arranged by size: 2349 1477 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4082 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4082 AvaI C'yCGr_G Cuts at: 783 912 949 1102 783 Size: 129 37 153 3763 Fragments arranged by size: 3763 153 129 37 BamHI G'GATC_C Cuts at: 1107 1107 Size: 4082 BanI G'GyrC_C Cuts at: 1098 1512 1760 1795 1098 Size: 414 248 35 3385 Fragments arranged by size: 3385 414 248 35 BanII G_rGCy'C Cuts at: 725 1031 1096 2126 725 Size: 306 65 1030 2681 Fragments arranged by size: 2681 1030 306 65 BbeI G_GCGC'C Cuts at: 1516 1764 1516 Size: 248 3834 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 117 151 117 Size: 34 4048 BciVI GTATCCnnnnn_n' Cuts at: 1980 2741 4082 1980 Size: 761 1341 1980 Fragments arranged by size: 1980 1341 761 BclI T'GATC_A Cuts at: 1602 1602 Size: 4082 BglI GCCn_nnn'nGGC Cuts at: 208 700 208 Size: 492 3590 BglII A'GATC_T Cuts at: 1597 3634 1597 Size: 2037 2045 BlpI GC'TnA_GC Cuts at: 1194 1194 Size: 4082 Bme1580I G_kGCm'C Cuts at: 464 542 1031 1707 1800 2856 464 Size: 78 489 676 93 1056 1690 Fragments arranged by size: 1690 1056 676 489 93 78 BmgBI CAC'GTC Cuts at: 531 537 531 Size: 6 4076 BmrI ACTGGGnnnn_n' Cuts at: 1294 1375 1710 3854 1294 Size: 81 335 2144 1522 Fragments arranged by size: 2144 1522 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 852 2449 852 Size: 1597 2485 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 571 571 Size: 4082 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 220 709 802 1258 2137 2629 2927 220 Size: 489 93 456 879 492 298 1375 Fragments arranged by size: 1375 879 492 489 456 298 93 BsaI GGTCTCn'nnnn_ Cuts at: 823 3786 823 Size: 2963 1119 BsaAI yAC'GTr Cuts at: 492 885 1435 492 Size: 393 550 3139 Fragments arranged by size: 3139 550 393 BsaBI GATnn'nnATC Cuts at: 6 1596 3633 6 Size: 1590 2037 455 Fragments arranged by size: 2037 1590 455 BsaHI Gr'CG_yC Cuts at: 1513 1761 2463 3959 1513 Size: 248 702 1496 1636 Fragments arranged by size: 1636 1496 702 248 BsaWI w'CCGG_w Cuts at: 1271 1792 2470 2744 2891 3302 1271 Size: 521 678 274 147 411 2051 Fragments arranged by size: 2051 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 457 487 544 574 457 Size: 30 57 30 3965 Fragments arranged by size: 3965 57 30 30 BseYI C'CCAG_C Cuts at: 904 1507 2265 2842 3338 904 Size: 603 758 577 496 1648 Fragments arranged by size: 1648 758 603 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 286 552 703 757 286 Size: 266 151 54 3611 Fragments arranged by size: 3611 266 151 54 BsiEI CG_ry'CG Cuts at: 104 628 695 1624 1670 2878 104 Size: 524 67 929 46 1208 1308 Fragments arranged by size: 1308 1208 929 524 67 46 BsiHKAI G_wGCw'C Cuts at: 671 806 893 998 1096 1874 2064 2856 671 Size: 135 87 105 98 778 190 792 1897 Fragments arranged by size: 1897 792 778 190 135 105 98 87 Bsp1286I G_dGCh'C Cuts at: 464 542 671 725 806 893 998 1031 1096 Size: 78 129 54 81 87 105 33 65 Cuts at: 1096 1707 1800 1874 2064 2126 2856 464 Size: 611 93 74 190 62 730 1690 Fragments arranged by size: 1690 730 611 190 129 105 93 87 81 78 74 65 62 54 33 BspEI T'CCGG_A Cuts at: 3302 3302 Size: 4082 BspHI T'CATG_A Cuts at: 3258 3258 Size: 4082 BspMI ACCTGCnnnn'nnnn_ Cuts at: 286 455 749 932 974 1132 1346 1648 2029 Size: 169 294 183 42 158 214 302 381 Cuts at: 2029 286 Size: 2339 Fragments arranged by size: 2339 381 302 294 214 183 169 158 42 BsrBI CCG'CTC Cuts at: 50 630 2374 2428 3912 4077 50 Size: 580 1744 54 1484 165 55 Fragments arranged by size: 1744 1484 580 165 55 54 BsrDI GCAATG_nn' Cuts at: 1994 1994 Size: 4082 BsrFI r'CCGG_y Cuts at: 2080 2261 2470 2080 Size: 181 209 3692 Fragments arranged by size: 3692 209 181 BsrGI T'GTAC_A Cuts at: 1052 1052 Size: 4082 BssSI C'ACGA_G Cuts at: 826 2353 2711 826 Size: 1527 358 2197 Fragments arranged by size: 2197 1527 358 BstAPI GCAn_nnn'nTGC Cuts at: 1459 1459 Size: 4082 BstBI TT'CG_AA Cuts at: 2443 2443 Size: 4082 BstXI CCAn_nnnn'nTGG Cuts at: 549 549 Size: 4082 BstYI r'GATC_y Cuts at: 1 1107 1574 1597 1932 2178 3190 3276 3288 Size: 1106 467 23 335 246 1012 86 12 Cuts at: 3288 3634 1 Size: 346 449 Fragments arranged by size: 1106 1012 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 452 731 107 Size: 345 279 3458 Fragments arranged by size: 3458 345 279 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2032 2213 2376 2032 Size: 181 163 3738 Fragments arranged by size: 3738 181 163 BtsI GCAGTG_nn' Cuts at: 1325 1325 Size: 4082 DraI TTT'AAA Cuts at: 1081 1081 Size: 4082 DraIII CAC_nnn'GTG Cuts at: 795 795 Size: 4082 DrdI GACnn_nn'nnGTC Cuts at: 1788 2646 1788 Size: 858 3224 EaeI y'GGCC_r Cuts at: 188 200 220 365 431 625 692 701 746 Size: 12 20 145 66 194 67 9 45 Cuts at: 746 797 1667 1841 2232 2259 3604 188 Size: 51 870 174 391 27 1345 666 Fragments arranged by size: 1345 870 666 391 194 174 145 67 66 51 45 27 20 12 9 EagI C'GGCC_G Cuts at: 625 692 1667 625 Size: 67 975 3040 Fragments arranged by size: 3040 975 67 EarI CTCTTCn'nnn_ Cuts at: 2105 2315 2105 Size: 210 3872 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2290 2600 2746 3892 2290 Size: 310 146 1146 2480 Fragments arranged by size: 2480 1146 310 146 EcoICRI GAG'CTC Cuts at: 1094 1094 Size: 4082 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 571 1907 2339 3086 3354 571 Size: 1336 432 747 268 1299 Fragments arranged by size: 1336 1299 747 432 268 EcoNI CCTnn'n_nnAGG Cuts at: 1035 1035 Size: 4082 EcoO109I rG'GnC_Cy Cuts at: 210 1027 1028 1221 210 Size: 817 1 193 3071 Fragments arranged by size: 3071 817 193 1 EcoRI G'AATT_C Cuts at: 1086 1086 Size: 4082 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2101 2101 Size: 4082 FspI TGC'GCA Cuts at: 1863 1863 Size: 4082 HaeII r_GCGC'y Cuts at: 309 1516 1764 2786 3765 309 Size: 1207 248 1022 979 626 Fragments arranged by size: 1207 1022 979 626 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1924 1954 1956 1986 1924 Size: 30 2 30 4020 Fragments arranged by size: 4020 30 30 2 HincII GTy'rAC Cuts at: 1121 1121 Size: 4082 KasI G'GCGC_C Cuts at: 1512 1760 1512 Size: 248 3834 KpnI G_GTAC'C Cuts at: 1102 1102 Size: 4082 MluI A'CGCG_T Cuts at: 544 3515 544 Size: 2971 1111 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2753 2937 2753 Size: 184 3898 MscI TGG'CCA Cuts at: 190 367 703 748 1843 190 Size: 177 336 45 1095 2429 Fragments arranged by size: 2429 1095 336 177 45 MslI CAynn'nnrTG Cuts at: 664 679 2071 2198 664 Size: 15 1392 127 2548 Fragments arranged by size: 2548 1392 127 15 MspA1I CmG'CkG Cuts at: 171 425 687 930 1193 1507 1867 2880 3125 Size: 254 262 243 263 314 360 1013 245 Cuts at: 3125 171 Size: 1128 Fragments arranged by size: 1128 1013 360 314 263 262 254 245 243 NaeI GCC'GGC Cuts at: 2263 2263 Size: 4082 NarI GG'CG_CC Cuts at: 1513 1761 1513 Size: 248 3834 NcoI C'CATG_G Cuts at: 107 107 Size: 4082 NgoMIV G'CCGG_C Cuts at: 2261 2261 Size: 4082 NspI r_CATG'y Cuts at: 813 1135 1635 2542 813 Size: 322 500 907 2353 Fragments arranged by size: 2353 907 500 322 PasI CC'CwG_GG Cuts at: 1023 1023 Size: 4082 PciI A'CATG_T Cuts at: 809 2538 809 Size: 1729 2353 PflMI CCAn_nnn'nTGG Cuts at: 795 795 Size: 4082 PmeI GTTT'AAAC Cuts at: 1081 1081 Size: 4082 PmlI CAC'GTG Cuts at: 492 885 492 Size: 393 3689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 544 576 3221 3253 544 Size: 32 2645 32 1373 Fragments arranged by size: 2645 1373 32 32 PspOMI G'GGCC_C Cuts at: 1027 1027 Size: 4082 PstI C_TGCA'G Cuts at: 605 1129 1814 605 Size: 524 685 2873 Fragments arranged by size: 2873 685 524 PvuI CG_AT'CG Cuts at: 104 104 Size: 4082 PvuII CAG'CTG Cuts at: 687 1507 1867 687 Size: 820 360 2902 Fragments arranged by size: 2902 820 360 RsrII CG'GwC_CG Cuts at: 2277 2277 Size: 4082 SacI G_AGCT'C Cuts at: 1096 1096 Size: 4082 SalI G'TCGA_C Cuts at: 1119 1119 Size: 4082 SapI GCTCTTCn'nnn_ Cuts at: 2105 2315 2105 Size: 210 3872 SbfI CC_TGCA'GG Cuts at: 1129 1129 Size: 4082 ScaI AGT'ACT Cuts at: 955 3599 955 Size: 2644 1438 SfcI C'TryA_G Cuts at: 33 601 1125 1810 2803 2994 33 Size: 568 524 685 993 191 1121 Fragments arranged by size: 1121 993 685 568 524 191 SfiI GGCCn_nnn'nGGCC Cuts at: 208 700 208 Size: 492 3590 SfoI GGC'GCC Cuts at: 1514 1762 1514 Size: 248 3834 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4082 SmaI CCC'GGG Cuts at: 914 1104 914 Size: 190 3892 SmlI C'TyrA_G Cuts at: 235 724 817 1237 2152 2644 2906 235 Size: 489 93 420 915 492 262 1411 Fragments arranged by size: 1411 915 492 489 420 262 93 SpeI A'CTAG_T Cuts at: 67 67 Size: 4082 SphI G_CATG'C Cuts at: 1135 1635 1135 Size: 500 3582 StyI C'CwwG_G Cuts at: 107 644 1216 1570 107 Size: 537 572 354 2619 Fragments arranged by size: 2619 572 537 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 445 975 1665 1798 2257 2466 445 Size: 530 690 133 459 209 2061 Fragments arranged by size: 2061 690 530 459 209 133 TatI w'GTAC_w Cuts at: 953 1052 3597 953 Size: 99 2545 1438 Fragments arranged by size: 2545 1438 99 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3599 3631 3599 Size: 32 4050 Tth111I GACn'n_nGTC Cuts at: 1879 1879 Size: 4082 XbaI T'CTAG_A Cuts at: 1113 1113 Size: 4082 XcmI CCAnnnn_n'nnnnTGG Cuts at: 450 756 1577 450 Size: 306 821 2955 Fragments arranged by size: 2955 821 306 XmaI C'CCGG_G Cuts at: 912 1102 912 Size: 190 3892 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AhdI AscI AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I BseRI BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PfoI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI