Restriction map for
pF1KE2762
(Circular) (Six-base) MAPSORT of: pF1KE2762.seq Check: 8866 from: 1 to: 4082
pF1KE2762 4082 bp
With 182 enzymes: SgfI *
October 3, 2019 17:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 974 974
Size: 4082
AccI GT'mk_AC
Cuts at: 146 737 1120 146
Size: 591 383 3108
Fragments arranged by size:
3108 591 383
Acc65I G'GTAC_C
Cuts at: 1098 1098
Size: 4082
AclI AA'CG_TT
Cuts at: 3922 3922
Size: 4082
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1907 2339 3086 3354 1907
Size: 432 747 268 2635
Fragments arranged by size:
2635 747 432 268
AflIII A'CryG_T
Cuts at: 544 809 1434 2538 3515 544
Size: 265 625 1104 977 1111
Fragments arranged by size:
1111 1104 977 625 265
AgeI A'CCGG_T
Cuts at: 2470 2470
Size: 4082
AleI CACnn'nnGTG
Cuts at: 679 679
Size: 4082
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 682 682
Size: 4082
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 544 576 544
Size: 32 4050
AlwNI CAG_nnn'CTG
Cuts at: 273 1387 2954 273
Size: 1114 1567 1401
Fragments arranged by size:
1567 1401 1114
ApaI G_GGCC'C
Cuts at: 1031 1031
Size: 4082
ApaLI G'TGCA_C
Cuts at: 2852 2852
Size: 4082
ApoI r'AATT_y
Cuts at: 1086 3435 3691 1086
Size: 2349 256 1477
Fragments arranged by size:
2349 1477 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4082
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
AvaI C'yCGr_G
Cuts at: 783 912 949 1102 783
Size: 129 37 153 3763
Fragments arranged by size:
3763 153 129 37
BamHI G'GATC_C
Cuts at: 1107 1107
Size: 4082
BanI G'GyrC_C
Cuts at: 1098 1512 1760 1795 1098
Size: 414 248 35 3385
Fragments arranged by size:
3385 414 248 35
BanII G_rGCy'C
Cuts at: 725 1031 1096 2126 725
Size: 306 65 1030 2681
Fragments arranged by size:
2681 1030 306 65
BbeI G_GCGC'C
Cuts at: 1516 1764 1516
Size: 248 3834
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 117 151 117
Size: 34 4048
BciVI GTATCCnnnnn_n'
Cuts at: 1980 2741 4082 1980
Size: 761 1341 1980
Fragments arranged by size:
1980 1341 761
BclI T'GATC_A
Cuts at: 1602 1602
Size: 4082
BglI GCCn_nnn'nGGC
Cuts at: 208 700 208
Size: 492 3590
BglII A'GATC_T
Cuts at: 1597 3634 1597
Size: 2037 2045
BlpI GC'TnA_GC
Cuts at: 1194 1194
Size: 4082
Bme1580I G_kGCm'C
Cuts at: 464 542 1031 1707 1800 2856 464
Size: 78 489 676 93 1056 1690
Fragments arranged by size:
1690 1056 676 489 93 78
BmgBI CAC'GTC
Cuts at: 531 537 531
Size: 6 4076
BmrI ACTGGGnnnn_n'
Cuts at: 1294 1375 1710 3854 1294
Size: 81 335 2144 1522
Fragments arranged by size:
2144 1522 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 852 2449 852
Size: 1597 2485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 571
Size: 4082
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 709 802 1258 2137 2629 2927 220
Size: 489 93 456 879 492 298 1375
Fragments arranged by size:
1375 879 492 489 456 298 93
BsaI GGTCTCn'nnnn_
Cuts at: 823 3786 823
Size: 2963 1119
BsaAI yAC'GTr
Cuts at: 492 885 1435 492
Size: 393 550 3139
Fragments arranged by size:
3139 550 393
BsaBI GATnn'nnATC
Cuts at: 6 1596 3633 6
Size: 1590 2037 455
Fragments arranged by size:
2037 1590 455
BsaHI Gr'CG_yC
Cuts at: 1513 1761 2463 3959 1513
Size: 248 702 1496 1636
Fragments arranged by size:
1636 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1271 1792 2470 2744 2891 3302 1271
Size: 521 678 274 147 411 2051
Fragments arranged by size:
2051 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 457 487 544 574 457
Size: 30 57 30 3965
Fragments arranged by size:
3965 57 30 30
BseYI C'CCAG_C
Cuts at: 904 1507 2265 2842 3338 904
Size: 603 758 577 496 1648
Fragments arranged by size:
1648 758 603 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 552 703 757 286
Size: 266 151 54 3611
Fragments arranged by size:
3611 266 151 54
BsiEI CG_ry'CG
Cuts at: 104 628 695 1624 1670 2878 104
Size: 524 67 929 46 1208 1308
Fragments arranged by size:
1308 1208 929 524 67 46
BsiHKAI G_wGCw'C
Cuts at: 671 806 893 998 1096 1874 2064 2856 671
Size: 135 87 105 98 778 190 792 1897
Fragments arranged by size:
1897 792 778 190 135 105 98 87
Bsp1286I G_dGCh'C
Cuts at: 464 542 671 725 806 893 998 1031 1096
Size: 78 129 54 81 87 105 33 65
Cuts at: 1096 1707 1800 1874 2064 2126 2856 464
Size: 611 93 74 190 62 730 1690
Fragments arranged by size:
1690 730 611 190 129 105 93 87
81 78 74 65 62 54 33
BspEI T'CCGG_A
Cuts at: 3302 3302
Size: 4082
BspHI T'CATG_A
Cuts at: 3258 3258
Size: 4082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 286 455 749 932 974 1132 1346 1648 2029
Size: 169 294 183 42 158 214 302 381
Cuts at: 2029 286
Size: 2339
Fragments arranged by size:
2339 381 302 294 214 183 169 158
42
BsrBI CCG'CTC
Cuts at: 50 630 2374 2428 3912 4077 50
Size: 580 1744 54 1484 165 55
Fragments arranged by size:
1744 1484 580 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1994 1994
Size: 4082
BsrFI r'CCGG_y
Cuts at: 2080 2261 2470 2080
Size: 181 209 3692
Fragments arranged by size:
3692 209 181
BsrGI T'GTAC_A
Cuts at: 1052 1052
Size: 4082
BssSI C'ACGA_G
Cuts at: 826 2353 2711 826
Size: 1527 358 2197
Fragments arranged by size:
2197 1527 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1459 1459
Size: 4082
BstBI TT'CG_AA
Cuts at: 2443 2443
Size: 4082
BstXI CCAn_nnnn'nTGG
Cuts at: 549 549
Size: 4082
BstYI r'GATC_y
Cuts at: 1 1107 1574 1597 1932 2178 3190 3276 3288
Size: 1106 467 23 335 246 1012 86 12
Cuts at: 3288 3634 1
Size: 346 449
Fragments arranged by size:
1106 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 452 731 107
Size: 345 279 3458
Fragments arranged by size:
3458 345 279
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2032 2213 2376 2032
Size: 181 163 3738
Fragments arranged by size:
3738 181 163
BtsI GCAGTG_nn'
Cuts at: 1325 1325
Size: 4082
DraI TTT'AAA
Cuts at: 1081 1081
Size: 4082
DraIII CAC_nnn'GTG
Cuts at: 795 795
Size: 4082
DrdI GACnn_nn'nnGTC
Cuts at: 1788 2646 1788
Size: 858 3224
EaeI y'GGCC_r
Cuts at: 188 200 220 365 431 625 692 701 746
Size: 12 20 145 66 194 67 9 45
Cuts at: 746 797 1667 1841 2232 2259 3604 188
Size: 51 870 174 391 27 1345 666
Fragments arranged by size:
1345 870 666 391 194 174 145 67
66 51 45 27 20 12 9
EagI C'GGCC_G
Cuts at: 625 692 1667 625
Size: 67 975 3040
Fragments arranged by size:
3040 975 67
EarI CTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2290 2600 2746 3892 2290
Size: 310 146 1146 2480
Fragments arranged by size:
2480 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1094 1094
Size: 4082
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 1907 2339 3086 3354 571
Size: 1336 432 747 268 1299
Fragments arranged by size:
1336 1299 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1035 1035
Size: 4082
EcoO109I rG'GnC_Cy
Cuts at: 210 1027 1028 1221 210
Size: 817 1 193 3071
Fragments arranged by size:
3071 817 193 1
EcoRI G'AATT_C
Cuts at: 1086 1086
Size: 4082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 4082
FspI TGC'GCA
Cuts at: 1863 1863
Size: 4082
HaeII r_GCGC'y
Cuts at: 309 1516 1764 2786 3765 309
Size: 1207 248 1022 979 626
Fragments arranged by size:
1207 1022 979 626 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1924 1954 1956 1986 1924
Size: 30 2 30 4020
Fragments arranged by size:
4020 30 30 2
HincII GTy'rAC
Cuts at: 1121 1121
Size: 4082
KasI G'GCGC_C
Cuts at: 1512 1760 1512
Size: 248 3834
KpnI G_GTAC'C
Cuts at: 1102 1102
Size: 4082
MluI A'CGCG_T
Cuts at: 544 3515 544
Size: 2971 1111
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2753 2937 2753
Size: 184 3898
MscI TGG'CCA
Cuts at: 190 367 703 748 1843 190
Size: 177 336 45 1095 2429
Fragments arranged by size:
2429 1095 336 177 45
MslI CAynn'nnrTG
Cuts at: 664 679 2071 2198 664
Size: 15 1392 127 2548
Fragments arranged by size:
2548 1392 127 15
MspA1I CmG'CkG
Cuts at: 171 425 687 930 1193 1507 1867 2880 3125
Size: 254 262 243 263 314 360 1013 245
Cuts at: 3125 171
Size: 1128
Fragments arranged by size:
1128 1013 360 314 263 262 254 245
243
NaeI GCC'GGC
Cuts at: 2263 2263
Size: 4082
NarI GG'CG_CC
Cuts at: 1513 1761 1513
Size: 248 3834
NcoI C'CATG_G
Cuts at: 107 107
Size: 4082
NgoMIV G'CCGG_C
Cuts at: 2261 2261
Size: 4082
NspI r_CATG'y
Cuts at: 813 1135 1635 2542 813
Size: 322 500 907 2353
Fragments arranged by size:
2353 907 500 322
PasI CC'CwG_GG
Cuts at: 1023 1023
Size: 4082
PciI A'CATG_T
Cuts at: 809 2538 809
Size: 1729 2353
PflMI CCAn_nnn'nTGG
Cuts at: 795 795
Size: 4082
PmeI GTTT'AAAC
Cuts at: 1081 1081
Size: 4082
PmlI CAC'GTG
Cuts at: 492 885 492
Size: 393 3689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 544 576 3221 3253 544
Size: 32 2645 32 1373
Fragments arranged by size:
2645 1373 32 32
PspOMI G'GGCC_C
Cuts at: 1027 1027
Size: 4082
PstI C_TGCA'G
Cuts at: 605 1129 1814 605
Size: 524 685 2873
Fragments arranged by size:
2873 685 524
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4082
PvuII CAG'CTG
Cuts at: 687 1507 1867 687
Size: 820 360 2902
Fragments arranged by size:
2902 820 360
RsrII CG'GwC_CG
Cuts at: 2277 2277
Size: 4082
SacI G_AGCT'C
Cuts at: 1096 1096
Size: 4082
SalI G'TCGA_C
Cuts at: 1119 1119
Size: 4082
SapI GCTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
SbfI CC_TGCA'GG
Cuts at: 1129 1129
Size: 4082
ScaI AGT'ACT
Cuts at: 955 3599 955
Size: 2644 1438
SfcI C'TryA_G
Cuts at: 33 601 1125 1810 2803 2994 33
Size: 568 524 685 993 191 1121
Fragments arranged by size:
1121 993 685 568 524 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 208 700 208
Size: 492 3590
SfoI GGC'GCC
Cuts at: 1514 1762 1514
Size: 248 3834
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
SmaI CCC'GGG
Cuts at: 914 1104 914
Size: 190 3892
SmlI C'TyrA_G
Cuts at: 235 724 817 1237 2152 2644 2906 235
Size: 489 93 420 915 492 262 1411
Fragments arranged by size:
1411 915 492 489 420 262 93
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4082
SphI G_CATG'C
Cuts at: 1135 1635 1135
Size: 500 3582
StyI C'CwwG_G
Cuts at: 107 644 1216 1570 107
Size: 537 572 354 2619
Fragments arranged by size:
2619 572 537 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 445 975 1665 1798 2257 2466 445
Size: 530 690 133 459 209 2061
Fragments arranged by size:
2061 690 530 459 209 133
TatI w'GTAC_w
Cuts at: 953 1052 3597 953
Size: 99 2545 1438
Fragments arranged by size:
2545 1438 99
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3599 3631 3599
Size: 32 4050
Tth111I GACn'n_nGTC
Cuts at: 1879 1879
Size: 4082
XbaI T'CTAG_A
Cuts at: 1113 1113
Size: 4082
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 450 756 1577 450
Size: 306 821 2955
Fragments arranged by size:
2955 821 306
XmaI C'CCGG_G
Cuts at: 912 1102 912
Size: 190 3892
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PmlI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AscI AvrII BaeI BbsI
BbvCI BfrBI BmtI Bpu10I BseRI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI