Restriction map for
pF1KE2790
(Circular) (Six-base) MAPSORT of: pF1KE2790.seq Check: 5285 from: 1 to: 3635
pF1KE2790 3635 bp
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 562 562
Size: 3635
AccI GT'mk_AC
Cuts at: 673 673
Size: 3635
Acc65I G'GTAC_C
Cuts at: 651 651
Size: 3635
AclI AA'CG_TT
Cuts at: 3475 3475
Size: 3635
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 523 1460 1892 2639 2907 502
Size: 21 937 432 747 268 1230
Fragments arranged by size:
1230 937 747 432 268 21
AflIII A'CryG_T
Cuts at: 987 2091 3068 987
Size: 1104 977 1554
Fragments arranged by size:
1554 1104 977
AgeI A'CCGG_T
Cuts at: 2023 2023
Size: 3635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 420 622 420
Size: 202 3433
AlwNI CAG_nnn'CTG
Cuts at: 940 2507 940
Size: 1567 2068
ApaLI G'TGCA_C
Cuts at: 2405 2405
Size: 3635
ApoI r'AATT_y
Cuts at: 639 2988 3244 639
Size: 2349 256 1030
Fragments arranged by size:
2349 1030 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3635
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3635
AvaI C'yCGr_G
Cuts at: 268 586 610 619 655 268
Size: 318 24 9 36 3248
Fragments arranged by size:
3248 318 36 24 9
BamHI G'GATC_C
Cuts at: 660 660
Size: 3635
BanI G'GyrC_C
Cuts at: 371 651 1065 1313 1348 371
Size: 280 414 248 35 2658
Fragments arranged by size:
2658 414 280 248 35
BanII G_rGCy'C
Cuts at: 215 649 1679 215
Size: 434 1030 2171
Fragments arranged by size:
2171 1030 434
BbeI G_GCGC'C
Cuts at: 375 1069 1317 375
Size: 694 248 2693
Fragments arranged by size:
2693 694 248
BbsI GAAGACnn'nnnn_
Cuts at: 490 490
Size: 3635
BciVI GTATCCnnnnn_n'
Cuts at: 1533 2294 3635 1533
Size: 761 1341 1533
Fragments arranged by size:
1533 1341 761
BclI T'GATC_A
Cuts at: 1155 1155
Size: 3635
BglII A'GATC_T
Cuts at: 1150 3187 1150
Size: 2037 1598
BlpI GC'TnA_GC
Cuts at: 747 747
Size: 3635
Bme1580I G_kGCm'C
Cuts at: 1260 1353 2409 1260
Size: 93 1056 2486
Fragments arranged by size:
2486 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 319 847 928 1263 3407 319
Size: 528 81 335 2144 547
Fragments arranged by size:
2144 547 528 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2002 2002
Size: 3635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 319
Size: 3635
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 1690 2182 2480 811
Size: 879 492 298 1966
Fragments arranged by size:
1966 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3339 3339
Size: 3635
BsaAI yAC'GTr
Cuts at: 988 988
Size: 3635
BsaBI GATnn'nnATC
Cuts at: 6 1149 3186 6
Size: 1143 2037 455
Fragments arranged by size:
2037 1143 455
BsaHI Gr'CG_yC
Cuts at: 372 1066 1314 2016 3512 372
Size: 694 248 702 1496 495
Fragments arranged by size:
1496 702 694 495 248
BsaWI w'CCGG_w
Cuts at: 563 824 1345 2023 2297 2444 2855 563
Size: 261 521 678 274 147 411 1343
Fragments arranged by size:
1343 678 521 411 274 261 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 122 253 370 511 122
Size: 131 117 141 3246
Fragments arranged by size:
3246 141 131 117
BseYI C'CCAG_C
Cuts at: 217 323 1060 1818 2395 2891 217
Size: 106 737 758 577 496 961
Fragments arranged by size:
961 758 737 577 496 106
BsiEI CG_ry'CG
Cuts at: 104 1177 1223 2431 104
Size: 1073 46 1208 1308
Fragments arranged by size:
1308 1208 1073 46
BsiHKAI G_wGCw'C
Cuts at: 649 1427 1617 2409 649
Size: 778 190 792 1875
Fragments arranged by size:
1875 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 215 649 1260 1353 1427 1617 1679 2409 215
Size: 434 611 93 74 190 62 730 1441
Fragments arranged by size:
1441 730 611 434 190 93 74 62
BspEI T'CCGG_A
Cuts at: 563 2855 563
Size: 2292 1343
BspHI T'CATG_A
Cuts at: 467 2811 467
Size: 2344 1291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 562 685 899 1201 1582 562
Size: 123 214 302 381 2615
Fragments arranged by size:
2615 381 302 214 123
BsrBI CCG'CTC
Cuts at: 50 456 513 1927 1981 3465 3630 50
Size: 406 57 1414 54 1484 165 55
Fragments arranged by size:
1484 1414 406 165 57 55 54
BsrDI GCAATG_nn'
Cuts at: 1547 1547
Size: 3635
BsrFI r'CCGG_y
Cuts at: 1633 1814 2023 1633
Size: 181 209 3245
Fragments arranged by size:
3245 209 181
BssSI C'ACGA_G
Cuts at: 1906 2264 1906
Size: 358 3277
BstAPI GCAn_nnn'nTGC
Cuts at: 1012 1012
Size: 3635
BstBI TT'CG_AA
Cuts at: 1996 1996
Size: 3635
BstEII G'GTnAC_C
Cuts at: 171 171
Size: 3635
BstYI r'GATC_y
Cuts at: 1 155 660 1127 1150 1485 1731 2743 2829
Size: 154 505 467 23 335 246 1012 86
Cuts at: 2829 2841 3187 1
Size: 12 346 449
Fragments arranged by size:
1012 505 467 449 346 335 246 154
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3635
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1585 1766 1929 1585
Size: 181 163 3291
Fragments arranged by size:
3291 181 163
BtsI GCAGTG_nn'
Cuts at: 604 878 604
Size: 274 3361
DraI TTT'AAA
Cuts at: 634 634
Size: 3635
DrdI GACnn_nn'nnGTC
Cuts at: 588 1341 2199 588
Size: 753 858 2024
Fragments arranged by size:
2024 858 753
EaeI y'GGCC_r
Cuts at: 1220 1394 1785 1812 3157 1220
Size: 174 391 27 1345 1698
Fragments arranged by size:
1698 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1220 1220
Size: 3635
EarI CTCTTCn'nnn_
Cuts at: 347 1658 1868 347
Size: 1311 210 2114
Fragments arranged by size:
2114 1311 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1843 2153 2299 3445 1843
Size: 310 146 1146 2033
Fragments arranged by size:
2033 1146 310 146
EcoICRI GAG'CTC
Cuts at: 647 647
Size: 3635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 502 523 1460 1892 2639 2907 319
Size: 183 21 937 432 747 268 1047
Fragments arranged by size:
1047 937 747 432 268 183 21
EcoO109I rG'GnC_Cy
Cuts at: 320 774 320
Size: 454 3181
EcoRI G'AATT_C
Cuts at: 639 639
Size: 3635
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1654 1654
Size: 3635
FspI TGC'GCA
Cuts at: 1416 1416
Size: 3635
HaeII r_GCGC'y
Cuts at: 375 1069 1317 2339 3318 375
Size: 694 248 1022 979 692
Fragments arranged by size:
1022 979 694 692 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1477 1507 1509 1539 1477
Size: 30 2 30 3573
Fragments arranged by size:
3573 30 30 2
HincII GTy'rAC
Cuts at: 674 674
Size: 3635
KasI G'GCGC_C
Cuts at: 371 1065 1313 371
Size: 694 248 2693
Fragments arranged by size:
2693 694 248
KpnI G_GTAC'C
Cuts at: 655 655
Size: 3635
MluI A'CGCG_T
Cuts at: 3068 3068
Size: 3635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2306 2490 2306
Size: 184 3451
MscI TGG'CCA
Cuts at: 1396 1396
Size: 3635
MslI CAynn'nnrTG
Cuts at: 529 1624 1751 529
Size: 1095 127 2413
Fragments arranged by size:
2413 1095 127
MspA1I CmG'CkG
Cuts at: 444 746 1060 1420 2433 2678 444
Size: 302 314 360 1013 245 1401
Fragments arranged by size:
1401 1013 360 314 302 245
NaeI GCC'GGC
Cuts at: 1816 1816
Size: 3635
NarI GG'CG_CC
Cuts at: 372 1066 1314 372
Size: 694 248 2693
Fragments arranged by size:
2693 694 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3635
NgoMIV G'CCGG_C
Cuts at: 1814 1814
Size: 3635
NspI r_CATG'y
Cuts at: 688 1188 2095 688
Size: 500 907 2228
Fragments arranged by size:
2228 907 500
PciI A'CATG_T
Cuts at: 2091 2091
Size: 3635
PflMI CCAn_nnn'nTGG
Cuts at: 196 196
Size: 3635
PfoI T'CCnGG_A
Cuts at: 158 296 158
Size: 138 3497
PmeI GTTT'AAAC
Cuts at: 634 634
Size: 3635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2774 2806 2774
Size: 32 3603
PspXI vC'TCGA_Gb
Cuts at: 610 610
Size: 3635
PstI C_TGCA'G
Cuts at: 443 682 1367 443
Size: 239 685 2711
Fragments arranged by size:
2711 685 239
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3635
PvuII CAG'CTG
Cuts at: 1060 1420 1060
Size: 360 3275
RsrII CG'GwC_CG
Cuts at: 566 1830 566
Size: 1264 2371
SacI G_AGCT'C
Cuts at: 649 649
Size: 3635
SalI G'TCGA_C
Cuts at: 672 672
Size: 3635
SapI GCTCTTCn'nnn_
Cuts at: 1658 1868 1658
Size: 210 3425
SbfI CC_TGCA'GG
Cuts at: 682 682
Size: 3635
ScaI AGT'ACT
Cuts at: 3152 3152
Size: 3635
SfcI C'TryA_G
Cuts at: 33 439 678 1363 2356 2547 33
Size: 406 239 685 993 191 1121
Fragments arranged by size:
1121 993 685 406 239 191
SfoI GGC'GCC
Cuts at: 373 1067 1315 373
Size: 694 248 2693
Fragments arranged by size:
2693 694 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3635
SmaI CCC'GGG
Cuts at: 657 657
Size: 3635
SmlI C'TyrA_G
Cuts at: 610 619 790 1705 2197 2459 610
Size: 9 171 915 492 262 1786
Fragments arranged by size:
1786 915 492 262 171 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3635
SphI G_CATG'C
Cuts at: 688 1188 688
Size: 500 3135
StyI C'CwwG_G
Cuts at: 107 769 1123 107
Size: 662 354 2619
Fragments arranged by size:
2619 662 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 583 1218 1351 1810 2019 583
Size: 635 133 459 209 2199
Fragments arranged by size:
2199 635 459 209 133
TatI w'GTAC_w
Cuts at: 3150 3150
Size: 3635
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 558 590 3152 3184 558
Size: 32 2562 32 1009
Fragments arranged by size:
2562 1009 32 32
Tth111I GACn'n_nGTC
Cuts at: 574 1432 574
Size: 858 2777
XbaI T'CTAG_A
Cuts at: 666 666
Size: 3635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1130 1130
Size: 3635
XhoI C'TCGA_G
Cuts at: 610 619 610
Size: 9 3626
XmaI C'CCGG_G
Cuts at: 655 655
Size: 3635
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PpuMI PshAI PsiI
PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XmnI ZraI