Restriction map for
pF1KE2794
(Circular) (Six-base) MAPSORT of: pF1KE2794.seq Check: 3464 from: 1 to: 5204
pF1KE2794 5204 bp
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AatII G_ACGT'C
Cuts at: 1832 1832
Size: 5204
AccI GT'mk_AC
Cuts at: 2242 2242
Size: 5204
Acc65I G'GTAC_C
Cuts at: 2220 2220
Size: 5204
AclI AA'CG_TT
Cuts at: 5044 5044
Size: 5204
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 1663 3029 3461 4208 4476 769
Size: 894 1366 432 747 268 1497
Fragments arranged by size:
1497 1366 894 747 432 268
AflIII A'CryG_T
Cuts at: 2556 3660 4637 2556
Size: 1104 977 3123
Fragments arranged by size:
3123 1104 977
AgeI A'CCGG_T
Cuts at: 3592 3592
Size: 5204
AhdI GACnn_n'nnGTC
Cuts at: 1320 1320
Size: 5204
AleI CACnn'nnGTG
Cuts at: 447 447
Size: 5204
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 406 814 1375 406
Size: 408 561 4235
Fragments arranged by size:
4235 561 408
AlwNI CAG_nnn'CTG
Cuts at: 316 1143 1254 1578 1617 1803 2509 4076 316
Size: 827 111 324 39 186 706 1567 1444
Fragments arranged by size:
1567 1444 827 706 324 186 111 39
ApaI G_GGCC'C
Cuts at: 349 1512 349
Size: 1163 4041
ApaLI G'TGCA_C
Cuts at: 3974 3974
Size: 5204
ApoI r'AATT_y
Cuts at: 1648 2208 4557 4813 1648
Size: 560 2349 256 2039
Fragments arranged by size:
2349 2039 560 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5204
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5204
AvaI C'yCGr_G
Cuts at: 183 335 395 824 1198 1238 1279 1347 1735
Size: 152 60 429 374 40 41 68 388
Cuts at: 1735 2224 183
Size: 489 3163
Fragments arranged by size:
3163 489 429 388 374 152 68 60
41 40
AvrII C'CTAG_G
Cuts at: 894 894
Size: 5204
BamHI G'GATC_C
Cuts at: 2229 2229
Size: 5204
BanI G'GyrC_C
Cuts at: 129 710 1885 2220 2634 2882 2917 129
Size: 581 1175 335 414 248 35 2416
Fragments arranged by size:
2416 1175 581 414 335 248 35
BanII G_rGCy'C
Cuts at: 183 223 349 960 1067 1345 1512 2218 3248
Size: 40 126 611 107 278 167 706 1030
Cuts at: 3248 183
Size: 2139
Fragments arranged by size:
2139 1030 706 611 278 167 126 107
40
BbeI G_GCGC'C
Cuts at: 2638 2886 2638
Size: 248 4956
BbsI GAAGACnn'nnnn_
Cuts at: 1120 1120
Size: 5204
BbvCI CC'TCA_GC
Cuts at: 274 1174 274
Size: 900 4304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 316 350 1081 1115 316
Size: 34 731 34 4405
Fragments arranged by size:
4405 731 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 553 1908 3102 3863 5204 553
Size: 1355 1194 761 1341 553
Fragments arranged by size:
1355 1341 1194 761 553
BclI T'GATC_A
Cuts at: 2724 2724
Size: 5204
BglI GCCn_nnn'nGGC
Cuts at: 875 1349 875
Size: 474 4730
BglII A'GATC_T
Cuts at: 329 1515 2719 4756 329
Size: 1186 1204 2037 777
Fragments arranged by size:
2037 1204 1186 777
BlpI GC'TnA_GC
Cuts at: 1726 2316 1726
Size: 590 4614
Bme1580I G_kGCm'C
Cuts at: 134 349 793 1354 1512 2829 2922 3978 134
Size: 215 444 561 158 1317 93 1056 1360
Fragments arranged by size:
1360 1317 1056 561 444 215 158 93
BmgBI CAC'GTC
Cuts at: 888 1131 888
Size: 243 4961
BmrI ACTGGGnnnn_n'
Cuts at: 2416 2497 2832 4976 2416
Size: 81 335 2144 2644
Fragments arranged by size:
2644 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 607 669 1392 3571 607
Size: 62 723 2179 2240
Fragments arranged by size:
2240 2179 723 62
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 774 1129 1639 2167 2193 774
Size: 355 510 528 26 3785
Fragments arranged by size:
3785 528 510 355 26
Bpu10I CC'TnA_GC
Cuts at: 136 274 1174 136
Size: 138 900 4166
Fragments arranged by size:
4166 900 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1289 2380 3259 3751 4049 1289
Size: 1091 879 492 298 2444
Fragments arranged by size:
2444 1091 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 623 1705 2143 4908 623
Size: 1082 438 2765 919
Fragments arranged by size:
2765 1082 919 438
BsaAI yAC'GTr
Cuts at: 2557 2557
Size: 5204
BsaBI GATnn'nnATC
Cuts at: 6 2718 4755 6
Size: 2712 2037 455
Fragments arranged by size:
2712 2037 455
BsaHI Gr'CG_yC
Cuts at: 839 905 1025 1541 1829 2635 2883 3585 5081
Size: 66 120 516 288 806 248 702 1496
Cuts at: 5081 839
Size: 962
Fragments arranged by size:
1496 962 806 702 516 288 248 120
66
BsaWI w'CCGG_w
Cuts at: 119 361 2078 2393 2914 3592 3866 4013 4424
Size: 242 1717 315 521 678 274 147 411
Cuts at: 4424 119
Size: 899
Fragments arranged by size:
1717 899 678 521 411 315 274 242
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1378 1408 1378
Size: 30 5174
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 191 1692 191
Size: 1501 3703
BseYI C'CCAG_C
Cuts at: 342 425 578 1694 1984 2629 3387 3964 4460
Size: 83 153 1116 290 645 758 577 496
Cuts at: 4460 342
Size: 1086
Fragments arranged by size:
1116 1086 758 645 577 496 290 153
83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1267 1887 1267
Size: 620 4584
BsiEI CG_ry'CG
Cuts at: 104 362 486 2746 2792 4000 104
Size: 258 124 2260 46 1208 1308
Fragments arranged by size:
2260 1308 1208 258 124 46
BsiHKAI G_wGCw'C
Cuts at: 410 2218 2996 3186 3978 410
Size: 1808 778 190 792 1636
Fragments arranged by size:
1808 1636 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 182 182
Size: 5204
Bsp1286I G_dGCh'C
Cuts at: 134 183 223 349 410 793 960 1067 1345
Size: 49 40 126 61 383 167 107 278
Cuts at: 1345 1354 1512 2218 2829 2922 2996 3186 3248
Size: 9 158 706 611 93 74 190 62
Cuts at: 3248 3978 134
Size: 730 1360
Fragments arranged by size:
1360 730 706 611 383 278 190 167
158 126 107 93 74 62 61 49
40 9
BspEI T'CCGG_A
Cuts at: 2078 4424 2078
Size: 2346 2858
BspHI T'CATG_A
Cuts at: 4380 4380
Size: 5204
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2254 2468 2770 3151 2254
Size: 214 302 381 4307
Fragments arranged by size:
4307 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1197 1437 3496 3550 5034 5199 50
Size: 1147 240 2059 54 1484 165 55
Fragments arranged by size:
2059 1484 1147 240 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3116 3116
Size: 5204
BsrFI r'CCGG_y
Cuts at: 707 854 3202 3383 3592 707
Size: 147 2348 181 209 2319
Fragments arranged by size:
2348 2319 209 181 147
BssSI C'ACGA_G
Cuts at: 1498 3475 3833 1498
Size: 1977 358 2869
Fragments arranged by size:
2869 1977 358
BstAPI GCAn_nnn'nTGC
Cuts at: 748 1254 2581 748
Size: 506 1327 3371
Fragments arranged by size:
3371 1327 506
BstBI TT'CG_AA
Cuts at: 3565 3565
Size: 5204
BstXI CCAn_nnnn'nTGG
Cuts at: 449 538 449
Size: 89 5115
BstYI r'GATC_y
Cuts at: 1 329 1515 2229 2696 2719 3054 3300 4312
Size: 328 1186 714 467 23 335 246 1012
Cuts at: 4312 4398 4410 4756 1
Size: 86 12 346 449
Fragments arranged by size:
1186 1012 714 467 449 346 335 328
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 250 350 250
Size: 100 5104
BtgI C'CryG_G
Cuts at: 107 297 920 1265 1393 1544 1787 1930 107
Size: 190 623 345 128 151 243 143 3381
Fragments arranged by size:
3381 623 345 243 190 151 143 128
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3154 3335 3498 3154
Size: 181 163 4860
Fragments arranged by size:
4860 181 163
BtsI GCAGTG_nn'
Cuts at: 531 2447 531
Size: 1916 3288
DraI TTT'AAA
Cuts at: 2203 2203
Size: 5204
DrdI GACnn_nn'nnGTC
Cuts at: 2910 3768 2910
Size: 858 4346
EaeI y'GGCC_r
Cuts at: 483 1046 1547 2789 2963 3354 3381 4726 483
Size: 563 501 1242 174 391 27 1345 961
Fragments arranged by size:
1345 1242 961 563 501 391 174 27
EagI C'GGCC_G
Cuts at: 483 2789 483
Size: 2306 2898
EarI CTCTTCn'nnn_
Cuts at: 168 638 3227 3437 168
Size: 470 2589 210 1935
Fragments arranged by size:
2589 1935 470 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 583 694 1047 1111 3412 3722 3868 5014 583
Size: 111 353 64 2301 310 146 1146 773
Fragments arranged by size:
2301 1146 773 353 310 146 111 64
EcoICRI GAG'CTC
Cuts at: 2216 2216
Size: 5204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 774 1129 1639 1663 2167 2193 3029 3461
Size: 5 355 510 24 504 26 836 432
Cuts at: 3461 4208 4476 769
Size: 747 268 1497
Fragments arranged by size:
1497 836 747 510 504 432 355 268
26 24 5
EcoO109I rG'GnC_Cy
Cuts at: 112 346 415 813 1052 1508 1926 2343 112
Size: 234 69 398 239 456 418 417 2973
Fragments arranged by size:
2973 456 418 417 398 239 234 69
EcoRI G'AATT_C
Cuts at: 2208 2208
Size: 5204
EcoRV GAT'ATC
Cuts at: 1185 1185
Size: 5204
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3223 3223
Size: 5204
FspI TGC'GCA
Cuts at: 1261 1363 2985 1261
Size: 102 1622 3480
Fragments arranged by size:
3480 1622 102
HaeII r_GCGC'y
Cuts at: 1408 1452 2638 2886 3908 4887 1408
Size: 44 1186 248 1022 979 1725
Fragments arranged by size:
1725 1186 1022 979 248 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 551 583 1306 1338 3046 3076 3078 3108 551
Size: 32 723 32 1708 30 2 30 2647
Fragments arranged by size:
2647 1708 723 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2243 2243
Size: 5204
HindIII A'AGCT_T
Cuts at: 1994 1994
Size: 5204
KasI G'GCGC_C
Cuts at: 2634 2882 2634
Size: 248 4956
KpnI G_GTAC'C
Cuts at: 2224 2224
Size: 5204
MluI A'CGCG_T
Cuts at: 4637 4637
Size: 5204
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 870 3875 4059 870
Size: 3005 184 2015
Fragments arranged by size:
3005 2015 184
MscI TGG'CCA
Cuts at: 1549 2965 1549
Size: 1416 3788
MslI CAynn'nnrTG
Cuts at: 447 3193 3320 447
Size: 2746 127 2331
Fragments arranged by size:
2746 2331 127
MspA1I CmG'CkG
Cuts at: 240 306 684 922 1039 1140 1152 1233 1461
Size: 66 378 238 117 101 12 81 228
Cuts at: 1461 1563 1664 2072 2315 2629 2989 4002 4247
Size: 102 101 408 243 314 360 1013 245
Cuts at: 4247 240
Size: 1197
Fragments arranged by size:
1197 1013 408 378 360 314 245 243
238 228 117 102 101 101 81 66
12
NaeI GCC'GGC
Cuts at: 709 3385 709
Size: 2676 2528
NarI GG'CG_CC
Cuts at: 2635 2883 2635
Size: 248 4956
NcoI C'CATG_G
Cuts at: 107 1930 107
Size: 1823 3381
NgoMIV G'CCGG_C
Cuts at: 707 3383 707
Size: 2676 2528
NspI r_CATG'y
Cuts at: 751 1421 2257 2757 3664 751
Size: 670 836 500 907 2291
Fragments arranged by size:
2291 907 836 670 500
PciI A'CATG_T
Cuts at: 3660 3660
Size: 5204
PflMI CCAn_nnn'nTGG
Cuts at: 1617 1617
Size: 5204
PfoI T'CCnGG_A
Cuts at: 833 833
Size: 5204
PmeI GTTT'AAAC
Cuts at: 2203 2203
Size: 5204
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4343 4375 4343
Size: 32 5172
PpuMI rG'GwC_Cy
Cuts at: 415 1926 415
Size: 1511 3693
PshAI GACnn'nnGTC
Cuts at: 1574 1574
Size: 5204
PspOMI G'GGCC_C
Cuts at: 345 1508 345
Size: 1163 4041
PspXI vC'TCGA_Gb
Cuts at: 1198 1279 1735 1198
Size: 81 456 4667
Fragments arranged by size:
4667 456 81
PstI C_TGCA'G
Cuts at: 695 729 770 803 1562 1853 2251 2936 695
Size: 34 41 33 759 291 398 685 2963
Fragments arranged by size:
2963 759 685 398 291 41 34 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5204
PvuII CAG'CTG
Cuts at: 306 1039 1140 1233 1461 1563 2072 2629 2989
Size: 733 101 93 228 102 509 557 360
Cuts at: 2989 306
Size: 2521
Fragments arranged by size:
2521 733 557 509 360 228 102 101
93
RsrII CG'GwC_CG
Cuts at: 3399 3399
Size: 5204
SacI G_AGCT'C
Cuts at: 2218 2218
Size: 5204
SacII CC_GC'GG
Cuts at: 923 923
Size: 5204
SalI G'TCGA_C
Cuts at: 2241 2241
Size: 5204
SapI GCTCTTCn'nnn_
Cuts at: 638 3227 3437 638
Size: 2589 210 2405
Fragments arranged by size:
2589 2405 210
SbfI CC_TGCA'GG
Cuts at: 770 2251 770
Size: 1481 3723
ScaI AGT'ACT
Cuts at: 4721 4721
Size: 5204
SfcI C'TryA_G
Cuts at: 33 691 725 766 799 1558 1849 2247 2932
Size: 658 34 41 33 759 291 398 685
Cuts at: 2932 3925 4116 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 759 685 658 398 291 191
41 34 33
SfoI GGC'GCC
Cuts at: 2636 2884 2636
Size: 248 4956
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5204
SmaI CCC'GGG
Cuts at: 185 826 1240 2226 185
Size: 641 414 986 3163
Fragments arranged by size:
3163 986 641 414
SmlI C'TyrA_G
Cuts at: 335 1198 1279 1304 1735 2359 3274 3766 4028
Size: 863 81 25 431 624 915 492 262
Cuts at: 4028 335
Size: 1511
Fragments arranged by size:
1511 915 863 624 492 431 262 81
25
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5204
SphI G_CATG'C
Cuts at: 751 2257 2757 751
Size: 1506 500 3198
Fragments arranged by size:
3198 1506 500
SrfI GCCC'GGGC
Cuts at: 826 826
Size: 5204
StyI C'CwwG_G
Cuts at: 107 537 623 864 894 1881 1930 2338 2692
Size: 430 86 241 30 987 49 408 354
Cuts at: 2692 107
Size: 2619
Fragments arranged by size:
2619 987 430 408 354 241 86 49
30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 193 455 1027 1331 2787 2920 3379 3588 193
Size: 262 572 304 1456 133 459 209 1809
Fragments arranged by size:
1809 1456 572 459 304 262 209 133
TatI w'GTAC_w
Cuts at: 4719 4719
Size: 5204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1378 1410 2083 2115 4721 4753 1378
Size: 32 673 32 2606 32 1829
Fragments arranged by size:
2606 1829 673 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 841 3001 841
Size: 2160 3044
XbaI T'CTAG_A
Cuts at: 1020 2235 1020
Size: 1215 3989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 871 967 2699 871
Size: 96 1732 3376
Fragments arranged by size:
3376 1732 96
XhoI C'TCGA_G
Cuts at: 335 1198 1279 1735 335
Size: 863 81 456 3804
Fragments arranged by size:
3804 863 456 81
XmaI C'CCGG_G
Cuts at: 183 824 1238 2224 183
Size: 641 414 986 3163
Fragments arranged by size:
3163 986 641 414
XmnI GAAnn'nnTTC
Cuts at: 1648 1648
Size: 5204
ZraI GAC'GTC
Cuts at: 1830 1830
Size: 5204
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AloI AscI BaeI BfrBI BmtI
BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoNI FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PsiI PsrI
SanDI SexAI SfiI SgrAI SnaBI SspI StuI SwaI