Restriction map for
pF1KE2802
(Circular) (Six-base) MAPSORT of: pF1KE2802.seq Check: 7137 from: 1 to: 3467
pF1KE2802 3467 bp
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AccI GT'mk_AC
Cuts at: 505 505
Size: 3467
Acc65I G'GTAC_C
Cuts at: 483 483
Size: 3467
AclI AA'CG_TT
Cuts at: 3307 3307
Size: 3467
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 1292 1724 2471 2739 208
Size: 1084 432 747 268 936
Fragments arranged by size:
1084 936 747 432 268
AflIII A'CryG_T
Cuts at: 113 819 1923 2900 113
Size: 706 1104 977 680
Fragments arranged by size:
1104 977 706 680
AgeI A'CCGG_T
Cuts at: 1855 1855
Size: 3467
AlwNI CAG_nnn'CTG
Cuts at: 237 772 2339 237
Size: 535 1567 1365
Fragments arranged by size:
1567 1365 535
ApaLI G'TGCA_C
Cuts at: 2237 2237
Size: 3467
ApoI r'AATT_y
Cuts at: 471 2820 3076 471
Size: 2349 256 862
Fragments arranged by size:
2349 862 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3467
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3467
AvaI C'yCGr_G
Cuts at: 429 487 429
Size: 58 3409
BamHI G'GATC_C
Cuts at: 492 492
Size: 3467
BanI G'GyrC_C
Cuts at: 131 483 897 1145 1180 131
Size: 352 414 248 35 2418
Fragments arranged by size:
2418 414 352 248 35
BanII G_rGCy'C
Cuts at: 481 1511 481
Size: 1030 2437
BbeI G_GCGC'C
Cuts at: 135 901 1149 135
Size: 766 248 2453
Fragments arranged by size:
2453 766 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 162 196 162
Size: 34 3433
BciVI GTATCCnnnnn_n'
Cuts at: 1365 2126 3467 1365
Size: 761 1341 1365
Fragments arranged by size:
1365 1341 761
BclI T'GATC_A
Cuts at: 987 987
Size: 3467
BglI GCCn_nnn'nGGC
Cuts at: 346 346
Size: 3467
BglII A'GATC_T
Cuts at: 982 3019 982
Size: 2037 1430
BlpI GC'TnA_GC
Cuts at: 579 579
Size: 3467
Bme1580I G_kGCm'C
Cuts at: 1092 1185 2241 1092
Size: 93 1056 2318
Fragments arranged by size:
2318 1056 93
BmgBI CAC'GTC
Cuts at: 114 114
Size: 3467
BmrI ACTGGGnnnn_n'
Cuts at: 286 679 760 1095 3239 286
Size: 393 81 335 2144 514
Fragments arranged by size:
2144 514 393 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1834 1834
Size: 3467
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 643 1522 2014 2312 196
Size: 447 879 492 298 1351
Fragments arranged by size:
1351 879 492 447 298
BsaI GGTCTCn'nnnn_
Cuts at: 3171 3171
Size: 3467
BsaAI yAC'GTr
Cuts at: 820 820
Size: 3467
BsaBI GATnn'nnATC
Cuts at: 6 981 3018 6
Size: 975 2037 455
Fragments arranged by size:
2037 975 455
BsaHI Gr'CG_yC
Cuts at: 132 158 898 1146 1848 3344 132
Size: 26 740 248 702 1496 255
Fragments arranged by size:
1496 740 702 255 248 26
BsaWI w'CCGG_w
Cuts at: 656 1177 1855 2129 2276 2687 656
Size: 521 678 274 147 411 1436
Fragments arranged by size:
1436 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 321 892 1650 2227 2723 321
Size: 571 758 577 496 1065
Fragments arranged by size:
1065 758 577 571 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 478
Size: 3467
BsiEI CG_ry'CG
Cuts at: 104 1009 1055 2263 104
Size: 905 46 1208 1308
Fragments arranged by size:
1308 1208 905 46
BsiHKAI G_wGCw'C
Cuts at: 481 1259 1449 2241 481
Size: 778 190 792 1707
Fragments arranged by size:
1707 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 481 1092 1185 1259 1449 1511 2241 481
Size: 611 93 74 190 62 730 1707
Fragments arranged by size:
1707 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2687 2687
Size: 3467
BspHI T'CATG_A
Cuts at: 122 2643 122
Size: 2521 946
BspMI ACCTGCnnnn'nnnn_
Cuts at: 450 517 731 1033 1414 450
Size: 67 214 302 381 2503
Fragments arranged by size:
2503 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 1759 1813 3297 3462 50
Size: 1709 54 1484 165 55
Fragments arranged by size:
1709 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1379 1379
Size: 3467
BsrFI r'CCGG_y
Cuts at: 1465 1646 1855 1465
Size: 181 209 3077
Fragments arranged by size:
3077 209 181
BsrGI T'GTAC_A
Cuts at: 239 239
Size: 3467
BssSI C'ACGA_G
Cuts at: 1738 2096 1738
Size: 358 3109
BstAPI GCAn_nnn'nTGC
Cuts at: 844 844
Size: 3467
BstBI TT'CG_AA
Cuts at: 182 1828 182
Size: 1646 1821
BstYI r'GATC_y
Cuts at: 1 492 959 982 1317 1563 2575 2661 2673
Size: 491 467 23 335 246 1012 86 12
Cuts at: 2673 3019 1
Size: 346 449
Fragments arranged by size:
1012 491 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3467
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1417 1598 1761 1417
Size: 181 163 3123
Fragments arranged by size:
3123 181 163
BtsI GCAGTG_nn'
Cuts at: 444 710 444
Size: 266 3201
DraI TTT'AAA
Cuts at: 466 466
Size: 3467
DrdI GACnn_nn'nnGTC
Cuts at: 1173 2031 1173
Size: 858 2609
EaeI y'GGCC_r
Cuts at: 310 1052 1226 1617 1644 2989 310
Size: 742 174 391 27 1345 788
Fragments arranged by size:
1345 788 742 391 174 27
EagI C'GGCC_G
Cuts at: 1052 1052
Size: 3467
EarI CTCTTCn'nnn_
Cuts at: 1490 1700 1490
Size: 210 3257
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1675 1985 2131 3277 1675
Size: 310 146 1146 1865
Fragments arranged by size:
1865 1146 310 146
EcoICRI GAG'CTC
Cuts at: 479 479
Size: 3467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 1292 1724 2471 2739 208
Size: 1084 432 747 268 936
Fragments arranged by size:
1084 936 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 318 366 606 318
Size: 48 240 3179
Fragments arranged by size:
3179 240 48
EcoRI G'AATT_C
Cuts at: 471 471
Size: 3467
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1486 1486
Size: 3467
FspI TGC'GCA
Cuts at: 1248 1248
Size: 3467
HaeII r_GCGC'y
Cuts at: 135 901 1149 2171 3150 135
Size: 766 248 1022 979 452
Fragments arranged by size:
1022 979 766 452 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1309 1339 1341 1371 1309
Size: 30 2 30 3405
Fragments arranged by size:
3405 30 30 2
HincII GTy'rAC
Cuts at: 506 506
Size: 3467
KasI G'GCGC_C
Cuts at: 131 897 1145 131
Size: 766 248 2453
Fragments arranged by size:
2453 766 248
KpnI G_GTAC'C
Cuts at: 487 487
Size: 3467
MluI A'CGCG_T
Cuts at: 2900 2900
Size: 3467
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2138 2322 2138
Size: 184 3283
MscI TGG'CCA
Cuts at: 1228 1228
Size: 3467
MslI CAynn'nnrTG
Cuts at: 112 1456 1583 112
Size: 1344 127 1996
Fragments arranged by size:
1996 1344 127
MspA1I CmG'CkG
Cuts at: 234 578 892 1252 2265 2510 234
Size: 344 314 360 1013 245 1191
Fragments arranged by size:
1191 1013 360 344 314 245
NaeI GCC'GGC
Cuts at: 1648 1648
Size: 3467
NarI GG'CG_CC
Cuts at: 132 898 1146 132
Size: 766 248 2453
Fragments arranged by size:
2453 766 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3467
NgoMIV G'CCGG_C
Cuts at: 1646 1646
Size: 3467
NspI r_CATG'y
Cuts at: 520 1020 1927 520
Size: 500 907 2060
Fragments arranged by size:
2060 907 500
PciI A'CATG_T
Cuts at: 1923 1923
Size: 3467
PflMI CCAn_nnn'nTGG
Cuts at: 258 258
Size: 3467
PmeI GTTT'AAAC
Cuts at: 466 466
Size: 3467
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2606 2638 2606
Size: 32 3435
PstI C_TGCA'G
Cuts at: 514 1199 514
Size: 685 2782
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3467
PvuII CAG'CTG
Cuts at: 892 1252 892
Size: 360 3107
RsrII CG'GwC_CG
Cuts at: 1662 1662
Size: 3467
SacI G_AGCT'C
Cuts at: 481 481
Size: 3467
SalI G'TCGA_C
Cuts at: 504 504
Size: 3467
SapI GCTCTTCn'nnn_
Cuts at: 1490 1700 1490
Size: 210 3257
SbfI CC_TGCA'GG
Cuts at: 514 514
Size: 3467
ScaI AGT'ACT
Cuts at: 2984 2984
Size: 3467
SfcI C'TryA_G
Cuts at: 33 510 1195 2188 2379 33
Size: 477 685 993 191 1121
Fragments arranged by size:
1121 993 685 477 191
SfoI GGC'GCC
Cuts at: 133 899 1147 133
Size: 766 248 2453
Fragments arranged by size:
2453 766 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3467
SmaI CCC'GGG
Cuts at: 489 489
Size: 3467
SmlI C'TyrA_G
Cuts at: 211 622 1537 2029 2291 211
Size: 411 915 492 262 1387
Fragments arranged by size:
1387 915 492 411 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3467
SphI G_CATG'C
Cuts at: 520 1020 520
Size: 500 2967
StyI C'CwwG_G
Cuts at: 107 161 601 955 107
Size: 54 440 354 2619
Fragments arranged by size:
2619 440 354 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1050 1183 1642 1851 1050
Size: 133 459 209 2666
Fragments arranged by size:
2666 459 209 133
TatI w'GTAC_w
Cuts at: 239 2982 239
Size: 2743 724
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2984 3016 2984
Size: 32 3435
Tth111I GACn'n_nGTC
Cuts at: 1264 1264
Size: 3467
XbaI T'CTAG_A
Cuts at: 498 498
Size: 3467
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 333 962 333
Size: 629 2838
XmaI C'CCGG_G
Cuts at: 487 487
Size: 3467
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmtI
BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI