Restriction map for
pF1KE3012
(Circular) (Six-base) MAPSORT of: pF1KE3012.seq Check: 3171 from: 1 to: 3416
pF1KE3012 3416 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 454 454
Size: 3416
Acc65I G'GTAC_C
Cuts at: 432 432
Size: 3416
AclI AA'CG_TT
Cuts at: 410 3256 410
Size: 2846 570
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 1241 1673 2420 2688 185
Size: 1056 432 747 268 913
Fragments arranged by size:
1056 913 747 432 268
AflIII A'CryG_T
Cuts at: 768 1872 2849 768
Size: 1104 977 1335
Fragments arranged by size:
1335 1104 977
AgeI A'CCGG_T
Cuts at: 1804 1804
Size: 3416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 215 215
Size: 3416
AlwNI CAG_nnn'CTG
Cuts at: 117 721 2288 117
Size: 604 1567 1245
Fragments arranged by size:
1567 1245 604
ApaI G_GGCC'C
Cuts at: 262 262
Size: 3416
ApaLI G'TGCA_C
Cuts at: 2186 2186
Size: 3416
ApoI r'AATT_y
Cuts at: 420 2769 3025 420
Size: 2349 256 811
Fragments arranged by size:
2349 811 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3416
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3416
AvaI C'yCGr_G
Cuts at: 436 436
Size: 3416
BamHI G'GATC_C
Cuts at: 441 441
Size: 3416
BanI G'GyrC_C
Cuts at: 432 846 1094 1129 432
Size: 414 248 35 2719
Fragments arranged by size:
2719 414 248 35
BanII G_rGCy'C
Cuts at: 262 430 1460 262
Size: 168 1030 2218
Fragments arranged by size:
2218 1030 168
BbeI G_GCGC'C
Cuts at: 850 1098 850
Size: 248 3168
BbvCI CC'TCA_GC
Cuts at: 398 398
Size: 3416
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3382
BciVI GTATCCnnnnn_n'
Cuts at: 1314 2075 3416 1314
Size: 761 1341 1314
Fragments arranged by size:
1341 1314 761
BclI T'GATC_A
Cuts at: 936 936
Size: 3416
BglII A'GATC_T
Cuts at: 931 2968 931
Size: 2037 1379
BlpI GC'TnA_GC
Cuts at: 528 528
Size: 3416
Bme1580I G_kGCm'C
Cuts at: 262 1041 1134 2190 262
Size: 779 93 1056 1488
Fragments arranged by size:
1488 1056 779 93
BmrI ACTGGGnnnn_n'
Cuts at: 628 709 1044 3188 628
Size: 81 335 2144 856
Fragments arranged by size:
2144 856 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1783 1783
Size: 3416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 268 367 253
Size: 15 99 3302
Fragments arranged by size:
3302 99 15
Bpu10I CC'TnA_GC
Cuts at: 398 398
Size: 3416
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 1471 1963 2261 592
Size: 879 492 298 1747
Fragments arranged by size:
1747 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3120 3120
Size: 3416
BsaAI yAC'GTr
Cuts at: 769 769
Size: 3416
BsaBI GATnn'nnATC
Cuts at: 6 930 2967 6
Size: 924 2037 455
Fragments arranged by size:
2037 924 455
BsaHI Gr'CG_yC
Cuts at: 847 1095 1797 3293 847
Size: 248 702 1496 970
Fragments arranged by size:
1496 970 702 248
BsaWI w'CCGG_w
Cuts at: 605 1126 1804 2078 2225 2636 605
Size: 521 678 274 147 411 1385
Fragments arranged by size:
1385 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 188 261 285 841 1599 2176 2672 188
Size: 73 24 556 758 577 496 932
Fragments arranged by size:
932 758 577 556 496 73 24
BsiEI CG_ry'CG
Cuts at: 104 387 958 1004 2212 104
Size: 283 571 46 1208 1308
Fragments arranged by size:
1308 1208 571 283 46
BsiHKAI G_wGCw'C
Cuts at: 430 1208 1398 2190 430
Size: 778 190 792 1656
Fragments arranged by size:
1656 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 262 430 1041 1134 1208 1398 1460 2190 262
Size: 168 611 93 74 190 62 730 1488
Fragments arranged by size:
1488 730 611 190 168 93 74 62
BspEI T'CCGG_A
Cuts at: 2636 2636
Size: 3416
BspHI T'CATG_A
Cuts at: 2592 2592
Size: 3416
BspMI ACCTGCnnnn'nnnn_
Cuts at: 466 680 982 1363 466
Size: 214 302 381 2519
Fragments arranged by size:
2519 381 302 214
BsrBI CCG'CTC
Cuts at: 50 185 1708 1762 3246 3411 50
Size: 135 1523 54 1484 165 55
Fragments arranged by size:
1523 1484 165 135 55 54
BsrDI GCAATG_nn'
Cuts at: 1328 1328
Size: 3416
BsrFI r'CCGG_y
Cuts at: 1414 1595 1804 1414
Size: 181 209 3026
Fragments arranged by size:
3026 209 181
BssSI C'ACGA_G
Cuts at: 310 1687 2045 310
Size: 1377 358 1681
Fragments arranged by size:
1681 1377 358
BstAPI GCAn_nnn'nTGC
Cuts at: 793 793
Size: 3416
BstBI TT'CG_AA
Cuts at: 1777 1777
Size: 3416
BstYI r'GATC_y
Cuts at: 1 441 908 931 1266 1512 2524 2610 2622
Size: 440 467 23 335 246 1012 86 12
Cuts at: 2622 2968 1
Size: 346 449
Fragments arranged by size:
1012 467 449 440 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 207 207
Size: 3416
BtgI C'CryG_G
Cuts at: 340 340
Size: 3416
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1366 1547 1710 1366
Size: 181 163 3072
Fragments arranged by size:
3072 181 163
BtsI GCAGTG_nn'
Cuts at: 399 659 399
Size: 260 3156
DraI TTT'AAA
Cuts at: 415 415
Size: 3416
DraIII CAC_nnn'GTG
Cuts at: 312 312
Size: 3416
DrdI GACnn_nn'nnGTC
Cuts at: 296 1122 1980 296
Size: 826 858 1732
Fragments arranged by size:
1732 858 826
EaeI y'GGCC_r
Cuts at: 152 384 1001 1175 1566 1593 2938 152
Size: 232 617 174 391 27 1345 630
Fragments arranged by size:
1345 630 617 391 232 174 27
EagI C'GGCC_G
Cuts at: 384 1001 384
Size: 617 2799
EarI CTCTTCn'nnn_
Cuts at: 1439 1649 1439
Size: 210 3206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1624 1934 2080 3226 1624
Size: 310 146 1146 1814
Fragments arranged by size:
1814 1146 310 146
EcoICRI GAG'CTC
Cuts at: 428 428
Size: 3416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 253 268 367 1241 1673 2420 2688 185
Size: 68 15 99 874 432 747 268 913
Fragments arranged by size:
913 874 747 432 268 99 68 15
EcoO109I rG'GnC_Cy
Cuts at: 228 258 289 298 555 228
Size: 30 31 9 257 3089
Fragments arranged by size:
3089 257 31 30 9
EcoRI G'AATT_C
Cuts at: 420 420
Size: 3416
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1435 1435
Size: 3416
FspI TGC'GCA
Cuts at: 1197 1197
Size: 3416
HaeII r_GCGC'y
Cuts at: 850 1098 2120 3099 850
Size: 248 1022 979 1167
Fragments arranged by size:
1167 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1258 1288 1290 1320 1258
Size: 30 2 30 3354
Fragments arranged by size:
3354 30 30 2
HincII GTy'rAC
Cuts at: 455 455
Size: 3416
HindIII A'AGCT_T
Cuts at: 167 167
Size: 3416
KasI G'GCGC_C
Cuts at: 846 1094 846
Size: 248 3168
KpnI G_GTAC'C
Cuts at: 436 436
Size: 3416
MluI A'CGCG_T
Cuts at: 2849 2849
Size: 3416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2087 2271 2087
Size: 184 3232
MscI TGG'CCA
Cuts at: 1177 1177
Size: 3416
MslI CAynn'nnrTG
Cuts at: 1405 1532 1405
Size: 127 3289
MspA1I CmG'CkG
Cuts at: 285 527 841 1201 2214 2459 285
Size: 242 314 360 1013 245 1242
Fragments arranged by size:
1242 1013 360 314 245 242
NaeI GCC'GGC
Cuts at: 1597 1597
Size: 3416
NarI GG'CG_CC
Cuts at: 847 1095 847
Size: 248 3168
NcoI C'CATG_G
Cuts at: 340 340
Size: 3416
NgoMIV G'CCGG_C
Cuts at: 1595 1595
Size: 3416
NspI r_CATG'y
Cuts at: 469 969 1876 469
Size: 500 907 2009
Fragments arranged by size:
2009 907 500
PasI CC'CwG_GG
Cuts at: 294 294
Size: 3416
PciI A'CATG_T
Cuts at: 1872 1872
Size: 3416
PmeI GTTT'AAAC
Cuts at: 415 415
Size: 3416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2555 2587 2555
Size: 32 3384
PpuMI rG'GwC_Cy
Cuts at: 228 289 298 228
Size: 61 9 3346
Fragments arranged by size:
3346 61 9
PspOMI G'GGCC_C
Cuts at: 258 258
Size: 3416
PstI C_TGCA'G
Cuts at: 463 1148 463
Size: 685 2731
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3416
PvuII CAG'CTG
Cuts at: 285 841 1201 285
Size: 556 360 2500
Fragments arranged by size:
2500 556 360
RsrII CG'GwC_CG
Cuts at: 1611 1611
Size: 3416
SacI G_AGCT'C
Cuts at: 430 430
Size: 3416
SalI G'TCGA_C
Cuts at: 453 453
Size: 3416
SanDI GG'GwC_CC
Cuts at: 289 298 289
Size: 9 3407
SapI GCTCTTCn'nnn_
Cuts at: 1439 1649 1439
Size: 210 3206
SbfI CC_TGCA'GG
Cuts at: 463 463
Size: 3416
ScaI AGT'ACT
Cuts at: 2933 2933
Size: 3416
SfcI C'TryA_G
Cuts at: 33 459 1144 2137 2328 33
Size: 426 685 993 191 1121
Fragments arranged by size:
1121 993 685 426 191
SfoI GGC'GCC
Cuts at: 848 1096 848
Size: 248 3168
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3416
SmaI CCC'GGG
Cuts at: 438 438
Size: 3416
SmlI C'TyrA_G
Cuts at: 571 1486 1978 2240 571
Size: 915 492 262 1747
Fragments arranged by size:
1747 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3416
SphI G_CATG'C
Cuts at: 469 969 469
Size: 500 2916
StyI C'CwwG_G
Cuts at: 340 550 904 340
Size: 210 354 2852
Fragments arranged by size:
2852 354 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 999 1132 1591 1800 999
Size: 133 459 209 2615
Fragments arranged by size:
2615 459 209 133
TatI w'GTAC_w
Cuts at: 2931 2931
Size: 3416
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2933 2965 2933
Size: 32 3384
Tth111I GACn'n_nGTC
Cuts at: 1213 1213
Size: 3416
XbaI T'CTAG_A
Cuts at: 447 447
Size: 3416
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 228 911 228
Size: 683 2733
XmaI C'CCGG_G
Cuts at: 436 436
Size: 3416
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BfrBI BglI BmgBI BmtI BsaXI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI