Restriction map for
pF1KE3105
(Circular) (Six-base) MAPSORT of: pF1KE3105.seq Check: 7198 from: 1 to: 3890
pF1KE3105 3890 bp
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AatII G_ACGT'C
Cuts at: 511 511
Size: 3890
AccI GT'mk_AC
Cuts at: 928 928
Size: 3890
Acc65I G'GTAC_C
Cuts at: 906 906
Size: 3890
AclI AA'CG_TT
Cuts at: 3730 3730
Size: 3890
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 611 1715 2147 2894 3162 178
Size: 433 1104 432 747 268 906
Fragments arranged by size:
1104 906 747 433 432 268
AflIII A'CryG_T
Cuts at: 416 1242 2346 3323 416
Size: 826 1104 977 983
Fragments arranged by size:
1104 983 977 826
AgeI A'CCGG_T
Cuts at: 2278 2278
Size: 3890
AlwNI CAG_nnn'CTG
Cuts at: 156 1195 2762 156
Size: 1039 1567 1284
Fragments arranged by size:
1567 1284 1039
ApaI G_GGCC'C
Cuts at: 487 487
Size: 3890
ApaLI G'TGCA_C
Cuts at: 2660 2660
Size: 3890
ApoI r'AATT_y
Cuts at: 316 894 3243 3499 316
Size: 578 2349 256 707
Fragments arranged by size:
2349 707 578 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3890
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3890
AvaI C'yCGr_G
Cuts at: 232 910 232
Size: 678 3212
BamHI G'GATC_C
Cuts at: 915 915
Size: 3890
BanI G'GyrC_C
Cuts at: 192 779 799 906 1320 1568 1603 192
Size: 587 20 107 414 248 35 2479
Fragments arranged by size:
2479 587 414 248 107 35 20
BanII G_rGCy'C
Cuts at: 135 215 461 487 764 904 1934 135
Size: 80 246 26 277 140 1030 2091
Fragments arranged by size:
2091 1030 277 246 140 80 26
BbeI G_GCGC'C
Cuts at: 803 1324 1572 803
Size: 521 248 3121
Fragments arranged by size:
3121 521 248
BbvCI CC'TCA_GC
Cuts at: 865 865
Size: 3890
BciVI GTATCCnnnnn_n'
Cuts at: 1788 2549 3890 1788
Size: 761 1341 1788
Fragments arranged by size:
1788 1341 761
BclI T'GATC_A
Cuts at: 347 1410 347
Size: 1063 2827
BglII A'GATC_T
Cuts at: 1405 3442 1405
Size: 2037 1853
BlpI GC'TnA_GC
Cuts at: 1002 1002
Size: 3890
Bme1580I G_kGCm'C
Cuts at: 487 1515 1608 2664 487
Size: 1028 93 1056 1713
Fragments arranged by size:
1713 1056 1028 93
BmgBI CAC'GTC
Cuts at: 672 672
Size: 3890
BmrI ACTGGGnnnn_n'
Cuts at: 329 464 1102 1183 1518 3662 329
Size: 135 638 81 335 2144 557
Fragments arranged by size:
2144 638 557 335 135 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2257 2257
Size: 3890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 748
Size: 3890
Bpu10I CC'TnA_GC
Cuts at: 463 865 463
Size: 402 3488
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1066 1945 2437 2735 1066
Size: 879 492 298 2221
Fragments arranged by size:
2221 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3594 3594
Size: 3890
BsaAI yAC'GTr
Cuts at: 419 861 1243 419
Size: 442 382 3066
Fragments arranged by size:
3066 442 382
BsaBI GATnn'nnATC
Cuts at: 6 1404 3441 6
Size: 1398 2037 455
Fragments arranged by size:
2037 1398 455
BsaHI Gr'CG_yC
Cuts at: 508 800 1321 1569 2271 3767 508
Size: 292 521 248 702 1496 631
Fragments arranged by size:
1496 702 631 521 292 248
BsaWI w'CCGG_w
Cuts at: 1079 1600 2278 2552 2699 3110 1079
Size: 521 678 274 147 411 1859
Fragments arranged by size:
1859 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 616 882 616
Size: 266 3624
BseYI C'CCAG_C
Cuts at: 1315 2073 2650 3146 1315
Size: 758 577 496 2059
Fragments arranged by size:
2059 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 559
Size: 3890
BsiEI CG_ry'CG
Cuts at: 104 1432 1478 2686 104
Size: 1328 46 1208 1308
Fragments arranged by size:
1328 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 904 1682 1872 2664 904
Size: 778 190 792 2130
Fragments arranged by size:
2130 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 499 682 499
Size: 183 3707
Bsp1286I G_dGCh'C
Cuts at: 135 215 461 487 764 904 1515 1608 1682
Size: 80 246 26 277 140 611 93 74
Cuts at: 1682 1872 1934 2664 135
Size: 190 62 730 1361
Fragments arranged by size:
1361 730 611 277 246 190 140 93
80 74 62 26
BspEI T'CCGG_A
Cuts at: 3110 3110
Size: 3890
BspHI T'CATG_A
Cuts at: 3066 3066
Size: 3890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 940 1154 1456 1837 940
Size: 214 302 381 2993
Fragments arranged by size:
2993 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2182 2236 3720 3885 50
Size: 2132 54 1484 165 55
Fragments arranged by size:
2132 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1802 1802
Size: 3890
BsrFI r'CCGG_y
Cuts at: 776 1888 2069 2278 776
Size: 1112 181 209 2388
Fragments arranged by size:
2388 1112 209 181
BssSI C'ACGA_G
Cuts at: 2161 2519 2161
Size: 358 3532
BstAPI GCAn_nnn'nTGC
Cuts at: 841 1267 841
Size: 426 3464
BstBI TT'CG_AA
Cuts at: 223 2251 223
Size: 2028 1862
BstYI r'GATC_y
Cuts at: 1 915 1382 1405 1740 1986 2998 3084 3096
Size: 914 467 23 335 246 1012 86 12
Cuts at: 3096 3442 1
Size: 346 449
Fragments arranged by size:
1012 914 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 140 783 140
Size: 643 3247
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 858 1840 2021 2184 858
Size: 982 181 163 2564
Fragments arranged by size:
2564 982 181 163
BtsI GCAGTG_nn'
Cuts at: 542 1133 542
Size: 591 3299
DraI TTT'AAA
Cuts at: 889 889
Size: 3890
DrdI GACnn_nn'nnGTC
Cuts at: 1596 2454 1596
Size: 858 3032
EaeI y'GGCC_r
Cuts at: 1475 1649 2040 2067 3412 1475
Size: 174 391 27 1345 1953
Fragments arranged by size:
1953 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1475 1475
Size: 3890
EarI CTCTTCn'nnn_
Cuts at: 1913 2123 1913
Size: 210 3680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2098 2408 2554 3700 2098
Size: 310 146 1146 2288
Fragments arranged by size:
2288 1146 310 146
EcoICRI GAG'CTC
Cuts at: 902 902
Size: 3890
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 611 748 1715 2147 2894 3162 178
Size: 433 137 967 432 747 268 906
Fragments arranged by size:
967 906 747 433 432 268 137
EcoNI CCTnn'n_nnAGG
Cuts at: 275 275
Size: 3890
EcoO109I rG'GnC_Cy
Cuts at: 483 484 1029 483
Size: 1 545 3344
Fragments arranged by size:
3344 545 1
EcoRI G'AATT_C
Cuts at: 894 894
Size: 3890
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1909 1909
Size: 3890
FspI TGC'GCA
Cuts at: 1671 1671
Size: 3890
HaeII r_GCGC'y
Cuts at: 803 1324 1572 2594 3573 803
Size: 521 248 1022 979 1120
Fragments arranged by size:
1120 1022 979 521 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1732 1762 1764 1794 1732
Size: 30 2 30 3828
Fragments arranged by size:
3828 30 30 2
HincII GTy'rAC
Cuts at: 929 929
Size: 3890
KasI G'GCGC_C
Cuts at: 799 1320 1568 799
Size: 521 248 3121
Fragments arranged by size:
3121 521 248
KpnI G_GTAC'C
Cuts at: 910 910
Size: 3890
MluI A'CGCG_T
Cuts at: 3323 3323
Size: 3890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 160 491 2561 2745 160
Size: 331 2070 184 1305
Fragments arranged by size:
2070 1305 331 184
MscI TGG'CCA
Cuts at: 1651 1651
Size: 3890
MslI CAynn'nnrTG
Cuts at: 1879 2006 1879
Size: 127 3763
MspA1I CmG'CkG
Cuts at: 717 1001 1315 1675 2688 2933 717
Size: 284 314 360 1013 245 1674
Fragments arranged by size:
1674 1013 360 314 284 245
NaeI GCC'GGC
Cuts at: 2071 2071
Size: 3890
NarI GG'CG_CC
Cuts at: 800 1321 1569 800
Size: 521 248 3121
Fragments arranged by size:
3121 521 248
NgoMIV G'CCGG_C
Cuts at: 2069 2069
Size: 3890
NspI r_CATG'y
Cuts at: 733 943 1443 2350 733
Size: 210 500 907 2273
Fragments arranged by size:
2273 907 500 210
PasI CC'CwG_GG
Cuts at: 492 492
Size: 3890
PciI A'CATG_T
Cuts at: 2346 2346
Size: 3890
PmeI GTTT'AAAC
Cuts at: 889 889
Size: 3890
PmlI CAC'GTG
Cuts at: 419 419
Size: 3890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3029 3061 3029
Size: 32 3858
PspOMI G'GGCC_C
Cuts at: 483 483
Size: 3890
PstI C_TGCA'G
Cuts at: 937 1622 937
Size: 685 3205
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3890
PvuII CAG'CTG
Cuts at: 717 1315 1675 717
Size: 598 360 2932
Fragments arranged by size:
2932 598 360
RsrII CG'GwC_CG
Cuts at: 2085 2085
Size: 3890
SacI G_AGCT'C
Cuts at: 904 904
Size: 3890
SalI G'TCGA_C
Cuts at: 927 927
Size: 3890
SapI GCTCTTCn'nnn_
Cuts at: 1913 2123 1913
Size: 210 3680
SbfI CC_TGCA'GG
Cuts at: 937 937
Size: 3890
ScaI AGT'ACT
Cuts at: 3407 3407
Size: 3890
SfcI C'TryA_G
Cuts at: 33 933 1618 2611 2802 33
Size: 900 685 993 191 1121
Fragments arranged by size:
1121 993 900 685 191
SfoI GGC'GCC
Cuts at: 801 1322 1570 801
Size: 521 248 3121
Fragments arranged by size:
3121 521 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3890
SmaI CCC'GGG
Cuts at: 912 912
Size: 3890
SmlI C'TyrA_G
Cuts at: 232 1045 1960 2452 2714 232
Size: 813 915 492 262 1408
Fragments arranged by size:
1408 915 813 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3890
SphI G_CATG'C
Cuts at: 733 943 1443 733
Size: 210 500 3180
Fragments arranged by size:
3180 500 210
SspI AAT'ATT
Cuts at: 228 228
Size: 3890
StyI C'CwwG_G
Cuts at: 1024 1378 1024
Size: 354 3536
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 784 1473 1606 2065 2274 784
Size: 689 133 459 209 2400
Fragments arranged by size:
2400 689 459 209 133
TatI w'GTAC_w
Cuts at: 3405 3405
Size: 3890
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3407 3439 3407
Size: 32 3858
Tth111I GACn'n_nGTC
Cuts at: 1687 1687
Size: 3890
XbaI T'CTAG_A
Cuts at: 921 921
Size: 3890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1385 1385
Size: 3890
XhoI C'TCGA_G
Cuts at: 232 232
Size: 3890
XmaI C'CCGG_G
Cuts at: 910 910
Size: 3890
XmnI GAAnn'nnTTC
Cuts at: 584 584
Size: 3890
ZraI GAC'GTC
Cuts at: 509 509
Size: 3890
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PmeI PmlI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmtI BsaXI
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI