(Circular) (Six-base) MAPSORT of: pF1KE3145.seq Check: 7407 from: 1 to: 4175 pF1KE3145 4175 bp With 182 enzymes: SgfI * December 4, 2012 11:59 .. AccI GT'mk_AC Cuts at: 1213 1213 Size: 4175 Acc65I G'GTAC_C Cuts at: 1191 1191 Size: 4175 AclI AA'CG_TT Cuts at: 4015 4015 Size: 4175 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 160 678 2000 2432 3179 3447 160 Size: 518 1322 432 747 268 888 Fragments arranged by size: 1322 888 747 518 432 268 AflIII A'CryG_T Cuts at: 1527 2631 3608 1527 Size: 1104 977 2094 Fragments arranged by size: 2094 1104 977 AgeI A'CCGG_T Cuts at: 2563 2563 Size: 4175 AlwNI CAG_nnn'CTG Cuts at: 601 866 1480 3047 601 Size: 265 614 1567 1729 Fragments arranged by size: 1729 1567 614 265 ApaLI G'TGCA_C Cuts at: 2945 2945 Size: 4175 ApoI r'AATT_y Cuts at: 512 1038 1061 1179 3528 3784 512 Size: 526 23 118 2349 256 903 Fragments arranged by size: 2349 903 526 256 118 23 AseI AT'TA_AT Cuts at: 20 20 Size: 4175 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4175 AvaI C'yCGr_G Cuts at: 638 1195 638 Size: 557 3618 BamHI G'GATC_C Cuts at: 1200 1200 Size: 4175 BanI G'GyrC_C Cuts at: 1191 1605 1853 1888 1191 Size: 414 248 35 3478 Fragments arranged by size: 3478 414 248 35 BanII G_rGCy'C Cuts at: 1189 2219 1189 Size: 1030 3145 BbeI G_GCGC'C Cuts at: 1609 1857 1609 Size: 248 3927 BbsI GAAGACnn'nnnn_ Cuts at: 821 821 Size: 4175 BbvCI CC'TCA_GC Cuts at: 479 479 Size: 4175 BciVI GTATCCnnnnn_n' Cuts at: 2073 2834 4175 2073 Size: 761 1341 2073 Fragments arranged by size: 2073 1341 761 BclI T'GATC_A Cuts at: 1695 1695 Size: 4175 BfrBI ATG'CAT Cuts at: 305 767 837 305 Size: 462 70 3643 Fragments arranged by size: 3643 462 70 BglII A'GATC_T Cuts at: 721 1690 3727 721 Size: 969 2037 1169 Fragments arranged by size: 2037 1169 969 BlpI GC'TnA_GC Cuts at: 188 1287 188 Size: 1099 3076 Bme1580I G_kGCm'C Cuts at: 1800 1893 2949 1800 Size: 93 1056 3026 Fragments arranged by size: 3026 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 989 1387 1468 1803 3947 989 Size: 398 81 335 2144 1217 Fragments arranged by size: 2144 1217 398 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2542 2542 Size: 4175 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 184 184 Size: 4175 Bpu10I CC'TnA_GC Cuts at: 479 479 Size: 4175 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1351 2230 2722 3020 1351 Size: 879 492 298 2506 Fragments arranged by size: 2506 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3879 3879 Size: 4175 BsaAI yAC'GTr Cuts at: 1528 1528 Size: 4175 BsaBI GATnn'nnATC Cuts at: 6 1689 3726 6 Size: 1683 2037 455 Fragments arranged by size: 2037 1683 455 BsaHI Gr'CG_yC Cuts at: 1606 1854 2556 4052 1606 Size: 248 702 1496 1729 Fragments arranged by size: 1729 1496 702 248 BsaWI w'CCGG_w Cuts at: 1364 1885 2563 2837 2984 3395 1364 Size: 521 678 274 147 411 2144 Fragments arranged by size: 2144 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 1600 2358 2935 3431 1600 Size: 758 577 496 2344 Fragments arranged by size: 2344 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 428 767 428 Size: 339 3836 BsiEI CG_ry'CG Cuts at: 104 1717 1763 2971 104 Size: 1613 46 1208 1308 Fragments arranged by size: 1613 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1189 1967 2157 2949 1189 Size: 778 190 792 2415 Fragments arranged by size: 2415 792 778 190 BsmI GAATG_Cn' Cuts at: 307 839 307 Size: 532 3643 Bsp1286I G_dGCh'C Cuts at: 1189 1800 1893 1967 2157 2219 2949 1189 Size: 611 93 74 190 62 730 2415 Fragments arranged by size: 2415 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3395 3395 Size: 4175 BspHI T'CATG_A Cuts at: 3351 3351 Size: 4175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1225 1439 1741 2122 1225 Size: 214 302 381 3278 Fragments arranged by size: 3278 381 302 214 BsrBI CCG'CTC Cuts at: 50 2467 2521 4005 4170 50 Size: 2417 54 1484 165 55 Fragments arranged by size: 2417 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 337 440 2087 337 Size: 103 1647 2425 Fragments arranged by size: 2425 1647 103 BsrFI r'CCGG_y Cuts at: 2173 2354 2563 2173 Size: 181 209 3785 Fragments arranged by size: 3785 209 181 BssSI C'ACGA_G Cuts at: 808 2446 2804 808 Size: 1638 358 2179 Fragments arranged by size: 2179 1638 358 BstAPI GCAn_nnn'nTGC Cuts at: 790 1552 790 Size: 762 3413 BstBI TT'CG_AA Cuts at: 2536 2536 Size: 4175 BstEII G'GTnAC_C Cuts at: 939 939 Size: 4175 BstXI CCAn_nnnn'nTGG Cuts at: 283 283 Size: 4175 BstYI r'GATC_y Cuts at: 1 504 721 1200 1667 1690 2025 2271 3283 Size: 503 217 479 467 23 335 246 1012 Cuts at: 3283 3369 3381 3727 1 Size: 86 12 346 449 Fragments arranged by size: 1012 503 479 467 449 346 335 246 217 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4175 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2125 2306 2469 2125 Size: 181 163 3831 Fragments arranged by size: 3831 181 163 BtsI GCAGTG_nn' Cuts at: 744 1418 744 Size: 674 3501 DraI TTT'AAA Cuts at: 681 1043 1174 681 Size: 362 131 3682 Fragments arranged by size: 3682 362 131 DraIII CAC_nnn'GTG Cuts at: 790 790 Size: 4175 DrdI GACnn_nn'nnGTC Cuts at: 1881 2739 1881 Size: 858 3317 EaeI y'GGCC_r Cuts at: 1760 1934 2325 2352 3697 1760 Size: 174 391 27 1345 2238 Fragments arranged by size: 2238 1345 391 174 27 EagI C'GGCC_G Cuts at: 1760 1760 Size: 4175 EarI CTCTTCn'nnn_ Cuts at: 2198 2408 2198 Size: 210 3965 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2383 2693 2839 3985 2383 Size: 310 146 1146 2573 Fragments arranged by size: 2573 1146 310 146 EcoICRI GAG'CTC Cuts at: 1187 1187 Size: 4175 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 160 184 678 2000 2432 3179 3447 160 Size: 24 494 1322 432 747 268 888 Fragments arranged by size: 1322 888 747 494 432 268 24 EcoO109I rG'GnC_Cy Cuts at: 1314 1314 Size: 4175 EcoRI G'AATT_C Cuts at: 512 1179 512 Size: 667 3508 EcoRV GAT'ATC Cuts at: 924 924 Size: 4175 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2194 2194 Size: 4175 FspI TGC'GCA Cuts at: 1956 1956 Size: 4175 HaeII r_GCGC'y Cuts at: 1609 1857 2879 3858 1609 Size: 248 1022 979 1926 Fragments arranged by size: 1926 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2017 2047 2049 2079 2017 Size: 30 2 30 4113 Fragments arranged by size: 4113 30 30 2 HincII GTy'rAC Cuts at: 1214 1214 Size: 4175 HindIII A'AGCT_T Cuts at: 172 172 Size: 4175 KasI G'GCGC_C Cuts at: 1605 1853 1605 Size: 248 3927 KpnI G_GTAC'C Cuts at: 1195 1195 Size: 4175 MluI A'CGCG_T Cuts at: 3608 3608 Size: 4175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2846 3030 2846 Size: 184 3991 MscI TGG'CCA Cuts at: 1936 1936 Size: 4175 MslI CAynn'nnrTG Cuts at: 281 2164 2291 281 Size: 1883 127 2165 Fragments arranged by size: 2165 1883 127 MspA1I CmG'CkG Cuts at: 1286 1600 1960 2973 3218 1286 Size: 314 360 1013 245 2243 Fragments arranged by size: 2243 1013 360 314 245 NaeI GCC'GGC Cuts at: 2356 2356 Size: 4175 NarI GG'CG_CC Cuts at: 1606 1854 1606 Size: 248 3927 NcoI C'CATG_G Cuts at: 107 107 Size: 4175 NgoMIV G'CCGG_C Cuts at: 2354 2354 Size: 4175 NsiI A_TGCA'T Cuts at: 307 769 839 307 Size: 462 70 3643 Fragments arranged by size: 3643 462 70 NspI r_CATG'y Cuts at: 767 1228 1728 2635 767 Size: 461 500 907 2307 Fragments arranged by size: 2307 907 500 461 PciI A'CATG_T Cuts at: 2631 2631 Size: 4175 PfoI T'CCnGG_A Cuts at: 507 507 Size: 4175 PmeI GTTT'AAAC Cuts at: 1174 1174 Size: 4175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3314 3346 3314 Size: 32 4143 PstI C_TGCA'G Cuts at: 1222 1907 1222 Size: 685 3490 PvuI CG_AT'CG Cuts at: 104 104 Size: 4175 PvuII CAG'CTG Cuts at: 1600 1960 1600 Size: 360 3815 RsrII CG'GwC_CG Cuts at: 2370 2370 Size: 4175 SacI G_AGCT'C Cuts at: 1189 1189 Size: 4175 SalI G'TCGA_C Cuts at: 1212 1212 Size: 4175 SapI GCTCTTCn'nnn_ Cuts at: 2198 2408 2198 Size: 210 3965 SbfI CC_TGCA'GG Cuts at: 1222 1222 Size: 4175 ScaI AGT'ACT Cuts at: 249 3692 249 Size: 3443 732 SfcI C'TryA_G Cuts at: 33 1218 1903 2896 3087 33 Size: 1185 685 993 191 1121 Fragments arranged by size: 1185 1121 993 685 191 SfoI GGC'GCC Cuts at: 1607 1855 1607 Size: 248 3927 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4175 SmaI CCC'GGG Cuts at: 1197 1197 Size: 4175 SmlI C'TyrA_G Cuts at: 638 1330 2245 2737 2999 638 Size: 692 915 492 262 1814 Fragments arranged by size: 1814 915 692 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4175 SphI G_CATG'C Cuts at: 1228 1728 1228 Size: 500 3675 StuI AGG'CCT Cuts at: 520 520 Size: 4175 StyI C'CwwG_G Cuts at: 107 378 944 1309 1663 107 Size: 271 566 365 354 2619 Fragments arranged by size: 2619 566 365 354 271 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1108 1758 1891 2350 2559 1108 Size: 650 133 459 209 2724 Fragments arranged by size: 2724 650 459 209 133 TatI w'GTAC_w Cuts at: 247 3690 247 Size: 3443 732 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3692 3724 3692 Size: 32 4143 Tth111I GACn'n_nGTC Cuts at: 802 1972 802 Size: 1170 3005 XbaI T'CTAG_A Cuts at: 1029 1206 1029 Size: 177 3998 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1670 1670 Size: 4175 XhoI C'TCGA_G Cuts at: 638 638 Size: 4175 XmaI C'CCGG_G Cuts at: 1195 1195 Size: 4175 XmnI GAAnn'nnTTC Cuts at: 123 845 123 Size: 722 3453 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BcgI BglI BmgBI BmtI BsaXI BseRI BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI FseI FspAI HpaI MfeI NdeI NheI NotI NruI PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI ZraI