Result of FASTA (omim) for
pF1KE3196
FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
Query: pF1KE3196, 470 aa
1>>>pF1KE3196 470 - 470 aa - 470 aa
Library: /omim/omim.rfq.tfa
64369986 residues in 92320 sequences
Statistics: Expectation_n fit: rho(ln(x))= 6.6004+/-0.000296; mu= 13.9480+/- 0.019
mean_var=141.8534+/-28.594, 0's: 0 Z-trim(120.8): 42 B-trim: 0 in 0/56
Lambda= 0.107685
statistics sampled from 37975 (38017) to 37975 sequences
Algorithm: FASTA (3.7 Nov 2010) [optimized]
Parameters: BL50 matrix (15:-5), open/ext: -10/-2
ktup: 2, E-join: 1 (0.746), E-opt: 0.2 (0.412), width: 16
Scan time: 5.140
The best scores are: opt bits E(92320)
NP_892010 (OMIM: 603730) sphingosine kinase 1 isof ( 470) 3206 509.5 8.8e-144
NP_001342068 (OMIM: 603730) sphingosine kinase 1 i ( 384) 2611 416.9 5.1e-116
NP_001136074 (OMIM: 603730) sphingosine kinase 1 i ( 384) 2611 416.9 5.1e-116
NP_001136073 (OMIM: 603730) sphingosine kinase 1 i ( 384) 2611 416.9 5.1e-116
NP_068807 (OMIM: 603730) sphingosine kinase 1 isof ( 398) 2586 413.1 7.8e-115
NP_001191087 (OMIM: 607092) sphingosine kinase 2 i ( 595) 851 143.7 1.4e-33
NP_001191089 (OMIM: 607092) sphingosine kinase 2 i ( 618) 851 143.7 1.5e-33
XP_006723355 (OMIM: 607092) sphingosine kinase 2 i ( 618) 851 143.7 1.5e-33
XP_011525436 (OMIM: 607092) sphingosine kinase 2 i ( 618) 851 143.7 1.5e-33
XP_011525435 (OMIM: 607092) sphingosine kinase 2 i ( 654) 851 143.7 1.5e-33
NP_064511 (OMIM: 607092) sphingosine kinase 2 isof ( 654) 851 143.7 1.5e-33
NP_001191088 (OMIM: 607092) sphingosine kinase 2 i ( 654) 851 143.7 1.5e-33
XP_016882497 (OMIM: 607092) sphingosine kinase 2 i ( 716) 851 143.8 1.6e-33
NP_001230805 (OMIM: 607092) sphingosine kinase 2 i ( 448) 730 124.8 5.3e-28
XP_016882499 (OMIM: 607092) sphingosine kinase 2 i ( 401) 472 84.7 5.7e-16
XP_016884398 (OMIM: 610307) ceramide kinase isofor ( 500) 320 61.1 8.6e-09
NP_073603 (OMIM: 610307) ceramide kinase [Homo sap ( 537) 320 61.2 9e-09
NP_001351877 (OMIM: 212350,610345,614691) acylglyc ( 350) 278 54.5 6.1e-07
XP_011514699 (OMIM: 212350,610345,614691) acylglyc ( 422) 278 54.5 7e-07
XP_024302603 (OMIM: 212350,610345,614691) acylglyc ( 422) 278 54.5 7e-07
NP_060708 (OMIM: 212350,610345,614691) acylglycero ( 422) 278 54.5 7e-07
NP_963842 (OMIM: 608380,608381) ceramide kinase-li ( 532) 199 42.4 0.0041
>>NP_892010 (OMIM: 603730) sphingosine kinase 1 isoform (470 aa)
initn: 3206 init1: 3206 opt: 3206 Z-score: 2702.9 bits: 509.5 E(92320): 8.8e-144
Smith-Waterman score: 3206; 100.0% identity (100.0% similar) in 470 aa overlap (1-470:1-470)
10 20 30 40 50 60
pF1KE3 MSAQVLGFLRSWTPLPLAAPRGPAAAGNDAGAPAATAPGGEGEPHSRPCDARLGSTDKEL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_892 MSAQVLGFLRSWTPLPLAAPRGPAAAGNDAGAPAATAPGGEGEPHSRPCDARLGSTDKEL
10 20 30 40 50 60
70 80 90 100 110 120
pF1KE3 KAGAAATGSAPTAPGTPWQREPRVEVMDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLF
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_892 KAGAAATGSAPTAPGTPWQREPRVEVMDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLF
70 80 90 100 110 120
130 140 150 160 170 180
pF1KE3 RSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMER
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_892 RSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMER
130 140 150 160 170 180
190 200 210 220 230 240
pF1KE3 PDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_892 PDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLS
190 200 210 220 230 240
250 260 270 280 290 300
pF1KE3 LHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLP
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_892 LHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLP
250 260 270 280 290 300
310 320 330 340 350 360
pF1KE3 VGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_892 VGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFA
310 320 330 340 350 360
370 380 390 400 410 420
pF1KE3 APMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_892 APMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDG
370 380 390 400 410 420
430 440 450 460 470
pF1KE3 KGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL
::::::::::::::::::::::::::::::::::::::::::::::::::
NP_892 KGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL
430 440 450 460 470
>>NP_001342068 (OMIM: 603730) sphingosine kinase 1 isofo (384 aa)
initn: 2611 init1: 2611 opt: 2611 Z-score: 2204.5 bits: 416.9 E(92320): 5.1e-116
Smith-Waterman score: 2611; 100.0% identity (100.0% similar) in 384 aa overlap (87-470:1-384)
60 70 80 90 100 110
pF1KE3 DKELKAGAAATGSAPTAPGTPWQREPRVEVMDPAGGPRGVLPRPCRVLVLLNPRGGKGKA
::::::::::::::::::::::::::::::
NP_001 MDPAGGPRGVLPRPCRVLVLLNPRGGKGKA
10 20 30
120 130 140 150 160 170
pF1KE3 LQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 LQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNG
40 50 60 70 80 90
180 190 200 210 220 230
pF1KE3 LMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPM
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 LMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPM
100 110 120 130 140 150
240 250 260 270 280 290
pF1KE3 NLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 NLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRL
160 170 180 190 200 210
300 310 320 330 340 350
pF1KE3 AYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 AYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGS
220 230 240 250 260 270
360 370 380 390 400 410
pF1KE3 EMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLE
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 EMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLE
280 290 300 310 320 330
420 430 440 450 460 470
pF1KE3 PKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL
::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 PKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL
340 350 360 370 380
>>NP_001136074 (OMIM: 603730) sphingosine kinase 1 isofo (384 aa)
initn: 2611 init1: 2611 opt: 2611 Z-score: 2204.5 bits: 416.9 E(92320): 5.1e-116
Smith-Waterman score: 2611; 100.0% identity (100.0% similar) in 384 aa overlap (87-470:1-384)
60 70 80 90 100 110
pF1KE3 DKELKAGAAATGSAPTAPGTPWQREPRVEVMDPAGGPRGVLPRPCRVLVLLNPRGGKGKA
::::::::::::::::::::::::::::::
NP_001 MDPAGGPRGVLPRPCRVLVLLNPRGGKGKA
10 20 30
120 130 140 150 160 170
pF1KE3 LQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 LQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNG
40 50 60 70 80 90
180 190 200 210 220 230
pF1KE3 LMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPM
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 LMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPM
100 110 120 130 140 150
240 250 260 270 280 290
pF1KE3 NLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 NLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRL
160 170 180 190 200 210
300 310 320 330 340 350
pF1KE3 AYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 AYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGS
220 230 240 250 260 270
360 370 380 390 400 410
pF1KE3 EMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLE
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 EMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLE
280 290 300 310 320 330
420 430 440 450 460 470
pF1KE3 PKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL
::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 PKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL
340 350 360 370 380
>>NP_001136073 (OMIM: 603730) sphingosine kinase 1 isofo (384 aa)
initn: 2611 init1: 2611 opt: 2611 Z-score: 2204.5 bits: 416.9 E(92320): 5.1e-116
Smith-Waterman score: 2611; 100.0% identity (100.0% similar) in 384 aa overlap (87-470:1-384)
60 70 80 90 100 110
pF1KE3 DKELKAGAAATGSAPTAPGTPWQREPRVEVMDPAGGPRGVLPRPCRVLVLLNPRGGKGKA
::::::::::::::::::::::::::::::
NP_001 MDPAGGPRGVLPRPCRVLVLLNPRGGKGKA
10 20 30
120 130 140 150 160 170
pF1KE3 LQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 LQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNG
40 50 60 70 80 90
180 190 200 210 220 230
pF1KE3 LMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPM
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 LMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPM
100 110 120 130 140 150
240 250 260 270 280 290
pF1KE3 NLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 NLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRL
160 170 180 190 200 210
300 310 320 330 340 350
pF1KE3 AYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 AYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGS
220 230 240 250 260 270
360 370 380 390 400 410
pF1KE3 EMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLE
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 EMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLE
280 290 300 310 320 330
420 430 440 450 460 470
pF1KE3 PKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL
::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 PKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL
340 350 360 370 380
>>NP_068807 (OMIM: 603730) sphingosine kinase 1 isoform (398 aa)
initn: 2586 init1: 2586 opt: 2586 Z-score: 2183.3 bits: 413.1 E(92320): 7.8e-115
Smith-Waterman score: 2586; 100.0% identity (100.0% similar) in 381 aa overlap (90-470:18-398)
60 70 80 90 100 110
pF1KE3 LKAGAAATGSAPTAPGTPWQREPRVEVMDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQL
::::::::::::::::::::::::::::::
NP_068 MDPVVGCGRGLFGFVFSAGGPRGVLPRPCRVLVLLNPRGGKGKALQL
10 20 30 40
120 130 140 150 160 170
pF1KE3 FRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLME
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_068 FRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLME
50 60 70 80 90 100
180 190 200 210 220 230
pF1KE3 RPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_068 RPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLL
110 120 130 140 150 160
240 250 260 270 280 290
pF1KE3 SLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_068 SLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYL
170 180 190 200 210 220
300 310 320 330 340 350
pF1KE3 PVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMF
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_068 PVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMF
230 240 250 260 270 280
360 370 380 390 400 410
pF1KE3 AAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKD
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_068 AAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKD
290 300 310 320 330 340
420 430 440 450 460 470
pF1KE3 GKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL
:::::::::::::::::::::::::::::::::::::::::::::::::::
NP_068 GKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL
350 360 370 380 390
>>NP_001191087 (OMIM: 607092) sphingosine kinase 2 isofo (595 aa)
initn: 1166 init1: 827 opt: 851 Z-score: 724.3 bits: 143.7 E(92320): 1.4e-33
Smith-Waterman score: 859; 44.6% identity (68.5% similar) in 336 aa overlap (14-332:22-350)
10 20 30 40
pF1KE3 MSAQVLGFLRSWTPLPLAAPRG---PAAAGNDAGAPAATAPGGEGE------
: : : ::: : : . . . .:. .
NP_001 MNGHLEAEEQQDQALHIQRLRPKPEARPRGGLVPLAEVSGCCTLRSRSPSDSAAYFCIYT
10 20 30 40 50 60
50 60 70 80 90
pF1KE3 -PHSRPCDARLGSTDKELKAGAAATGSAPTAPGTPWQREPRVEVMD---PAGG---PRGV
:..: :: .: . ..: .::: : . : . :. : : .
NP_001 YPRGRR-GARRRAT-RTFRADGAATYEENRAEAQRWATALTCLLRGLPLPGDGEITP-DL
70 80 90 100 110
100 110 120 130 140 150
pF1KE3 LPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELG
:::: :.:.:.:: ::.: : : ..:: :...:: .::.:. :::.:::::::.. :.
NP_001 LPRPPRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGLSFNLIQTERQNHARELVQGLSLS
120 130 140 150 160 170
160 170 180 190 200 210
pF1KE3 RWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVT
.::..:..:::::.:::.:::..::::: :.. :. :: :::::::...:...:.: .
NP_001 EWDGIVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGILPCGSGNALAGAVNQHGGFEPAL
180 190 200 210 220 230
220 230 240 250 260 270
pF1KE3 NEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEM
. ::: ::.::::: :..:::. ::: : :: ::.::::..:::..::..: ::
NP_001 GLDLLLNCSLLLCRGGGHPLDLLSVTLASGSRCFSFLSVAWGFVSDVDIQSERFRALGSA
240 250 260 270 280 290
280 290 300 310 320 330
pF1KE3 RFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGP-VDAHLVPLEEPVPSHW
:::::: : ::.:.::::::.:::. . ::::. ... : . ..:. .:.:
NP_001 RFTLGTVLGLATLHTYRGRLSYLPA----TVEPASPTPAHSLPRAKSELTLTPDPAPPMA
300 310 320 330 340 350
340 350 360 370 380 390
pF1KE3 TVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKG
NP_001 HSPLHRSVSDLPLPLPQPALASPGSPEPLPILSLNGGGPELAGDWGGAGDAPLSPDPLLS
360 370 380 390 400 410
>--
initn: 417 init1: 353 opt: 423 Z-score: 365.0 bits: 77.2 E(92320): 1.5e-13
Smith-Waterman score: 423; 45.4% identity (66.3% similar) in 163 aa overlap (300-460:445-595)
270 280 290 300 310 320
pF1KE3 YRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEE
: .:::..: :: : : ::
NP_001 PPGSPKAALHSPVSEGAPVIPPSSGLPLPTPDARVGAST--------CGPPDHLLPPLGT
420 430 440 450 460
330 340 350 360 370 380
pF1KE3 PVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLF
:.: : :. . ::::.::. ::::... ::: .: :..:: .::.:.::: :::::
NP_001 PLPPDW-VTLEGDFVLMLAISPSHLGADLVAAPHARFDDGLVHLCWVRSGISRAALLRLF
470 480 490 500 510 520
390 400 410 420 430 440
pF1KE3 LAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVS
::::.: :. :: : :. . :::::: .::..:::: . .:.:.::. ...
NP_001 LAMERGSHFSLGCPQLGYAAARAFRLEPLTPRGVLTVDGEQVEYGPLQAQMHPGIGTLLT
530 540 550 560 570 580
450 460 470
pF1KE3 GCVEPP--PSWKPQQMPPPEEPL
: :: :. .:
NP_001 G---PPGCPGREP
590
>>NP_001191089 (OMIM: 607092) sphingosine kinase 2 isofo (618 aa)
initn: 1166 init1: 827 opt: 851 Z-score: 724.1 bits: 143.7 E(92320): 1.5e-33
Smith-Waterman score: 865; 43.8% identity (68.2% similar) in 349 aa overlap (1-332:33-373)
10 20
pF1KE3 MSAQVLGFLRSWTPLPLAAPRG---PAAAG
...:.: . : : : : ::: : :
NP_001 PPPPPLAASTPLLHGEFGSYPARGPRFALTLTSQALHIQR-LRPKPEARPRGGLVPLAEV
10 20 30 40 50 60
30 40 50 60 70 80
pF1KE3 NDAGAPAATAPGGEGE-------PHSRPCDARLGSTDKELKAGAAATGSAPTAPGTPWQR
. . . .:. . :..: :: .: . ..: .::: : . :
NP_001 SGCCTLRSRSPSDSAAYFCIYTYPRGRR-GARRRAT-RTFRADGAATYEENRAEAQRWAT
70 80 90 100 110
90 100 110 120 130
pF1KE3 EPRVEVMD---PAGG---PRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEIS
. :. : : .:::: :.:.:.:: ::.: : : ..:: :...:: .:
NP_001 ALTCLLRGLPLPGDGEITP-DLLPRPPRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGLS
120 130 140 150 160 170
140 150 160 170 180 190
pF1KE3 FTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSL
:.:. :::.:::::::.. :..::..:..:::::.:::.:::..::::: :.. :. :
NP_001 FNLIQTERQNHARELVQGLSLSEWDGIVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGIL
180 190 200 210 220 230
200 210 220 230 240 250
pF1KE3 PAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLS
: :::::::...:...:.: . . ::: ::.::::: :..:::. ::: : :: ::
NP_001 PCGSGNALAGAVNQHGGFEPALGLDLLLNCSLLLCRGGGHPLDLLSVTLASGSRCFSFLS
240 250 260 270 280 290
260 270 280 290 300 310
pF1KE3 LAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVV
.::::..:::..::..: :: :::::: : ::.:.::::::.:::. . ::::.
NP_001 VAWGFVSDVDIQSERFRALGSARFTLGTVLGLATLHTYRGRLSYLPA----TVEPASPTP
300 310 320 330 340 350
320 330 340 350 360 370
pF1KE3 VQQGP-VDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHL
... : . ..:. .:.:
NP_001 AHSLPRAKSELTLTPDPAPPMAHSPLHRSVSDLPLPLPQPALASPGSPEPLPILSLNGGG
360 370 380 390 400 410
>--
initn: 417 init1: 353 opt: 423 Z-score: 364.7 bits: 77.2 E(92320): 1.5e-13
Smith-Waterman score: 423; 45.4% identity (66.3% similar) in 163 aa overlap (300-460:468-618)
270 280 290 300 310 320
pF1KE3 YRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEE
: .:::..: :: : : ::
NP_001 PPGSPKAALHSPVSEGAPVIPPSSGLPLPTPDARVGAST--------CGPPDHLLPPLGT
440 450 460 470 480
330 340 350 360 370 380
pF1KE3 PVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLF
:.: : :. . ::::.::. ::::... ::: .: :..:: .::.:.::: :::::
NP_001 PLPPDW-VTLEGDFVLMLAISPSHLGADLVAAPHARFDDGLVHLCWVRSGISRAALLRLF
490 500 510 520 530 540
390 400 410 420 430 440
pF1KE3 LAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVS
::::.: :. :: : :. . :::::: .::..:::: . .:.:.::. ...
NP_001 LAMERGSHFSLGCPQLGYAAARAFRLEPLTPRGVLTVDGEQVEYGPLQAQMHPGIGTLLT
550 560 570 580 590 600
450 460 470
pF1KE3 GCVEPP--PSWKPQQMPPPEEPL
: :: :. .:
NP_001 G---PPGCPGREP
610
>>XP_006723355 (OMIM: 607092) sphingosine kinase 2 isofo (618 aa)
initn: 1166 init1: 827 opt: 851 Z-score: 724.1 bits: 143.7 E(92320): 1.5e-33
Smith-Waterman score: 865; 43.8% identity (68.2% similar) in 349 aa overlap (1-332:33-373)
10 20
pF1KE3 MSAQVLGFLRSWTPLPLAAPRG---PAAAG
...:.: . : : : : ::: : :
XP_006 PPPPPLAASTPLLHGEFGSYPARGPRFALTLTSQALHIQR-LRPKPEARPRGGLVPLAEV
10 20 30 40 50 60
30 40 50 60 70 80
pF1KE3 NDAGAPAATAPGGEGE-------PHSRPCDARLGSTDKELKAGAAATGSAPTAPGTPWQR
. . . .:. . :..: :: .: . ..: .::: : . :
XP_006 SGCCTLRSRSPSDSAAYFCIYTYPRGRR-GARRRAT-RTFRADGAATYEENRAEAQRWAT
70 80 90 100 110
90 100 110 120 130
pF1KE3 EPRVEVMD---PAGG---PRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEIS
. :. : : .:::: :.:.:.:: ::.: : : ..:: :...:: .:
XP_006 ALTCLLRGLPLPGDGEITP-DLLPRPPRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGLS
120 130 140 150 160 170
140 150 160 170 180 190
pF1KE3 FTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSL
:.:. :::.:::::::.. :..::..:..:::::.:::.:::..::::: :.. :. :
XP_006 FNLIQTERQNHARELVQGLSLSEWDGIVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGIL
180 190 200 210 220 230
200 210 220 230 240 250
pF1KE3 PAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLS
: :::::::...:...:.: . . ::: ::.::::: :..:::. ::: : :: ::
XP_006 PCGSGNALAGAVNQHGGFEPALGLDLLLNCSLLLCRGGGHPLDLLSVTLASGSRCFSFLS
240 250 260 270 280 290
260 270 280 290 300 310
pF1KE3 LAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVV
.::::..:::..::..: :: :::::: : ::.:.::::::.:::. . ::::.
XP_006 VAWGFVSDVDIQSERFRALGSARFTLGTVLGLATLHTYRGRLSYLPA----TVEPASPTP
300 310 320 330 340 350
320 330 340 350 360 370
pF1KE3 VQQGP-VDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHL
... : . ..:. .:.:
XP_006 AHSLPRAKSELTLTPDPAPPMAHSPLHRSVSDLPLPLPQPALASPGSPEPLPILSLNGGG
360 370 380 390 400 410
>--
initn: 417 init1: 353 opt: 423 Z-score: 364.7 bits: 77.2 E(92320): 1.5e-13
Smith-Waterman score: 423; 45.4% identity (66.3% similar) in 163 aa overlap (300-460:468-618)
270 280 290 300 310 320
pF1KE3 YRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEE
: .:::..: :: : : ::
XP_006 PPGSPKAALHSPVSEGAPVIPPSSGLPLPTPDARVGAST--------CGPPDHLLPPLGT
440 450 460 470 480
330 340 350 360 370 380
pF1KE3 PVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLF
:.: : :. . ::::.::. ::::... ::: .: :..:: .::.:.::: :::::
XP_006 PLPPDW-VTLEGDFVLMLAISPSHLGADLVAAPHARFDDGLVHLCWVRSGISRAALLRLF
490 500 510 520 530 540
390 400 410 420 430 440
pF1KE3 LAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVS
::::.: :. :: : :. . :::::: .::..:::: . .:.:.::. ...
XP_006 LAMERGSHFSLGCPQLGYAAARAFRLEPLTPRGVLTVDGEQVEYGPLQAQMHPGIGTLLT
550 560 570 580 590 600
450 460 470
pF1KE3 GCVEPP--PSWKPQQMPPPEEPL
: :: :. .:
XP_006 G---PPGCPGREP
610
>>XP_011525436 (OMIM: 607092) sphingosine kinase 2 isofo (618 aa)
initn: 1166 init1: 827 opt: 851 Z-score: 724.1 bits: 143.7 E(92320): 1.5e-33
Smith-Waterman score: 865; 43.8% identity (68.2% similar) in 349 aa overlap (1-332:33-373)
10 20
pF1KE3 MSAQVLGFLRSWTPLPLAAPRG---PAAAG
...:.: . : : : : ::: : :
XP_011 PPPPPLAASTPLLHGEFGSYPARGPRFALTLTSQALHIQR-LRPKPEARPRGGLVPLAEV
10 20 30 40 50 60
30 40 50 60 70 80
pF1KE3 NDAGAPAATAPGGEGE-------PHSRPCDARLGSTDKELKAGAAATGSAPTAPGTPWQR
. . . .:. . :..: :: .: . ..: .::: : . :
XP_011 SGCCTLRSRSPSDSAAYFCIYTYPRGRR-GARRRAT-RTFRADGAATYEENRAEAQRWAT
70 80 90 100 110
90 100 110 120 130
pF1KE3 EPRVEVMD---PAGG---PRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEIS
. :. : : .:::: :.:.:.:: ::.: : : ..:: :...:: .:
XP_011 ALTCLLRGLPLPGDGEITP-DLLPRPPRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGLS
120 130 140 150 160 170
140 150 160 170 180 190
pF1KE3 FTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSL
:.:. :::.:::::::.. :..::..:..:::::.:::.:::..::::: :.. :. :
XP_011 FNLIQTERQNHARELVQGLSLSEWDGIVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGIL
180 190 200 210 220 230
200 210 220 230 240 250
pF1KE3 PAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLS
: :::::::...:...:.: . . ::: ::.::::: :..:::. ::: : :: ::
XP_011 PCGSGNALAGAVNQHGGFEPALGLDLLLNCSLLLCRGGGHPLDLLSVTLASGSRCFSFLS
240 250 260 270 280 290
260 270 280 290 300 310
pF1KE3 LAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVV
.::::..:::..::..: :: :::::: : ::.:.::::::.:::. . ::::.
XP_011 VAWGFVSDVDIQSERFRALGSARFTLGTVLGLATLHTYRGRLSYLPA----TVEPASPTP
300 310 320 330 340 350
320 330 340 350 360 370
pF1KE3 VQQGP-VDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHL
... : . ..:. .:.:
XP_011 AHSLPRAKSELTLTPDPAPPMAHSPLHRSVSDLPLPLPQPALASPGSPEPLPILSLNGGG
360 370 380 390 400 410
>--
initn: 417 init1: 353 opt: 423 Z-score: 364.7 bits: 77.2 E(92320): 1.5e-13
Smith-Waterman score: 423; 45.4% identity (66.3% similar) in 163 aa overlap (300-460:468-618)
270 280 290 300 310 320
pF1KE3 YRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEE
: .:::..: :: : : ::
XP_011 PPGSPKAALHSPVSEGAPVIPPSSGLPLPTPDARVGAST--------CGPPDHLLPPLGT
440 450 460 470 480
330 340 350 360 370 380
pF1KE3 PVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLF
:.: : :. . ::::.::. ::::... ::: .: :..:: .::.:.::: :::::
XP_011 PLPPDW-VTLEGDFVLMLAISPSHLGADLVAAPHARFDDGLVHLCWVRSGISRAALLRLF
490 500 510 520 530 540
390 400 410 420 430 440
pF1KE3 LAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVS
::::.: :. :: : :. . :::::: .::..:::: . .:.:.::. ...
XP_011 LAMERGSHFSLGCPQLGYAAARAFRLEPLTPRGVLTVDGEQVEYGPLQAQMHPGIGTLLT
550 560 570 580 590 600
450 460 470
pF1KE3 GCVEPP--PSWKPQQMPPPEEPL
: :: :. .:
XP_011 G---PPGCPGREP
610
>>XP_011525435 (OMIM: 607092) sphingosine kinase 2 isofo (654 aa)
initn: 1190 init1: 827 opt: 851 Z-score: 723.8 bits: 143.7 E(92320): 1.5e-33
Smith-Waterman score: 865; 43.8% identity (68.2% similar) in 349 aa overlap (1-332:69-409)
10 20
pF1KE3 MSAQVLGFLRSWTPLPLAAPRG---PAAAG
...:.: . : : : : ::: : :
XP_011 PPPPPLAASTPLLHGEFGSYPARGPRFALTLTSQALHIQR-LRPKPEARPRGGLVPLAEV
40 50 60 70 80 90
30 40 50 60 70 80
pF1KE3 NDAGAPAATAPGGEGE-------PHSRPCDARLGSTDKELKAGAAATGSAPTAPGTPWQR
. . . .:. . :..: :: .: . ..: .::: : . :
XP_011 SGCCTLRSRSPSDSAAYFCIYTYPRGRR-GARRRAT-RTFRADGAATYEENRAEAQRWAT
100 110 120 130 140 150
90 100 110 120 130
pF1KE3 EPRVEVMD---PAGG---PRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEIS
. :. : : .:::: :.:.:.:: ::.: : : ..:: :...:: .:
XP_011 ALTCLLRGLPLPGDGEITP-DLLPRPPRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGLS
160 170 180 190 200 210
140 150 160 170 180 190
pF1KE3 FTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSL
:.:. :::.:::::::.. :..::..:..:::::.:::.:::..::::: :.. :. :
XP_011 FNLIQTERQNHARELVQGLSLSEWDGIVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGIL
220 230 240 250 260 270
200 210 220 230 240 250
pF1KE3 PAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLS
: :::::::...:...:.: . . ::: ::.::::: :..:::. ::: : :: ::
XP_011 PCGSGNALAGAVNQHGGFEPALGLDLLLNCSLLLCRGGGHPLDLLSVTLASGSRCFSFLS
280 290 300 310 320 330
260 270 280 290 300 310
pF1KE3 LAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVV
.::::..:::..::..: :: :::::: : ::.:.::::::.:::. . ::::.
XP_011 VAWGFVSDVDIQSERFRALGSARFTLGTVLGLATLHTYRGRLSYLPA----TVEPASPTP
340 350 360 370 380 390
320 330 340 350 360 370
pF1KE3 VQQGP-VDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHL
... : . ..:. .:.:
XP_011 AHSLPRAKSELTLTPDPAPPMAHSPLHRSVSDLPLPLPQPALASPGSPEPLPILSLNGGG
400 410 420 430 440 450
>--
initn: 417 init1: 353 opt: 423 Z-score: 364.4 bits: 77.2 E(92320): 1.6e-13
Smith-Waterman score: 423; 45.4% identity (66.3% similar) in 163 aa overlap (300-460:504-654)
270 280 290 300 310 320
pF1KE3 YRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEE
: .:::..: :: : : ::
XP_011 PPGSPKAALHSPVSEGAPVIPPSSGLPLPTPDARVGAST--------CGPPDHLLPPLGT
480 490 500 510 520
330 340 350 360 370 380
pF1KE3 PVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLF
:.: : :. . ::::.::. ::::... ::: .: :..:: .::.:.::: :::::
XP_011 PLPPDW-VTLEGDFVLMLAISPSHLGADLVAAPHARFDDGLVHLCWVRSGISRAALLRLF
530 540 550 560 570 580
390 400 410 420 430 440
pF1KE3 LAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVS
::::.: :. :: : :. . :::::: .::..:::: . .:.:.::. ...
XP_011 LAMERGSHFSLGCPQLGYAAARAFRLEPLTPRGVLTVDGEQVEYGPLQAQMHPGIGTLLT
590 600 610 620 630 640
450 460 470
pF1KE3 GCVEPP--PSWKPQQMPPPEEPL
: :: :. .:
XP_011 G---PPGCPGREP
650
470 residues in 1 query sequences
64369986 residues in 92320 library sequences
Tcomplib [36.3.4 Apr, 2011] (8 proc)
start: Thu Oct 24 21:47:45 2019 done: Thu Oct 24 21:47:46 2019
Total Scan time: 5.140 Total Display time: 0.080
Function used was FASTA [36.3.4 Apr, 2011]