Restriction map for
pF1KE3196
(Circular) (Six-base) MAPSORT of: pF1KE3196.seq Check: 8214 from: 1 to: 4523
pF1KE3196 4523 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1041 1041
Size: 4523
AccI GT'mk_AC
Cuts at: 1561 1561
Size: 4523
Acc65I G'GTAC_C
Cuts at: 1539 1539
Size: 4523
AclI AA'CG_TT
Cuts at: 4363 4363
Size: 4523
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 2348 2780 3527 3795 307
Size: 2041 432 747 268 1035
Fragments arranged by size:
2041 1035 747 432 268
AflIII A'CryG_T
Cuts at: 1875 2979 3956 1875
Size: 1104 977 2442
Fragments arranged by size:
2442 1104 977
AgeI A'CCGG_T
Cuts at: 2911 2911
Size: 4523
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 472 802 973 472
Size: 330 171 4022
Fragments arranged by size:
4022 330 171
AlwNI CAG_nnn'CTG
Cuts at: 700 1828 3395 700
Size: 1128 1567 1828
Fragments arranged by size:
1828 1567 1128
ApaI G_GGCC'C
Cuts at: 1063 1063
Size: 4523
ApaLI G'TGCA_C
Cuts at: 1426 3293 1426
Size: 1867 2656
ApoI r'AATT_y
Cuts at: 1527 3876 4132 1527
Size: 2349 256 1918
Fragments arranged by size:
2349 1918 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4523
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4523
AvaI C'yCGr_G
Cuts at: 165 386 1543 165
Size: 221 1157 3145
Fragments arranged by size:
3145 1157 221
BamHI G'GATC_C
Cuts at: 369 1548 369
Size: 1179 3344
BanI G'GyrC_C
Cuts at: 949 1119 1539 1953 2201 2236 949
Size: 170 420 414 248 35 3236
Fragments arranged by size:
3236 420 414 248 170 35
BanII G_rGCy'C
Cuts at: 239 638 1063 1358 1469 1514 1537 2567 239
Size: 399 425 295 111 45 23 1030 2195
Fragments arranged by size:
2195 1030 425 399 295 111 45 23
BbeI G_GCGC'C
Cuts at: 1957 2205 1957
Size: 248 4275
BbsI GAAGACnn'nnnn_
Cuts at: 767 767
Size: 4523
BbvCI CC'TCA_GC
Cuts at: 494 494
Size: 4523
BciVI GTATCCnnnnn_n'
Cuts at: 2421 3182 4523 2421
Size: 761 1341 2421
Fragments arranged by size:
2421 1341 761
BclI T'GATC_A
Cuts at: 2043 2043
Size: 4523
BfrBI ATG'CAT
Cuts at: 1221 1221
Size: 4523
BglI GCCn_nnn'nGGC
Cuts at: 389 389
Size: 4523
BglII A'GATC_T
Cuts at: 2038 4075 2038
Size: 2037 2486
BlpI GC'TnA_GC
Cuts at: 865 1635 865
Size: 770 3753
Bme1580I G_kGCm'C
Cuts at: 952 1063 1103 1124 1334 1430 2148 2241 3297
Size: 111 40 21 210 96 718 93 1056
Cuts at: 3297 952
Size: 2178
Fragments arranged by size:
2178 1056 718 210 111 96 93 40
21
BmrI ACTGGGnnnn_n'
Cuts at: 663 1735 1816 2151 4295 663
Size: 1072 81 335 2144 891
Fragments arranged by size:
2144 1072 891 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2890 2890
Size: 4523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 623 1108 623
Size: 485 4038
Bpu10I CC'TnA_GC
Cuts at: 494 494
Size: 4523
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 1699 2578 3070 3368 101
Size: 1598 879 492 298 1256
Fragments arranged by size:
1598 1256 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 652 4227 652
Size: 3575 948
BsaAI yAC'GTr
Cuts at: 477 1233 1876 477
Size: 756 643 3124
Fragments arranged by size:
3124 756 643
BsaBI GATnn'nnATC
Cuts at: 6 2037 4074 6
Size: 2031 2037 455
Fragments arranged by size:
2037 2031 455
BsaHI Gr'CG_yC
Cuts at: 194 257 921 1214 1954 2202 2904 4400 194
Size: 63 664 293 740 248 702 1496 317
Fragments arranged by size:
1496 740 702 664 317 293 248 63
BsaWI w'CCGG_w
Cuts at: 467 1712 2233 2911 3185 3332 3743 467
Size: 1245 521 678 274 147 411 1247
Fragments arranged by size:
1247 1245 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 580 1105 580
Size: 525 3998
BseYI C'CCAG_C
Cuts at: 570 578 690 1474 1487 1948 2706 3283 3779
Size: 8 112 784 13 461 758 577 496
Cuts at: 3779 570
Size: 1314
Fragments arranged by size:
1314 784 758 577 496 461 112 13
8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 762 814 1141 1172 1226 1435 499
Size: 263 52 327 31 54 209 3587
Fragments arranged by size:
3587 327 263 209 54 52 31
BsiEI CG_ry'CG
Cuts at: 104 562 990 2065 2111 3319 104
Size: 458 428 1075 46 1208 1308
Fragments arranged by size:
1308 1208 1075 458 428 46
BsiHKAI G_wGCw'C
Cuts at: 204 398 866 1430 1537 2315 2505 3297 204
Size: 194 468 564 107 778 190 792 1430
Fragments arranged by size:
1430 792 778 564 468 194 190 107
BsmI GAATG_Cn'
Cuts at: 1315 1315
Size: 4523
BsmBI CGTCTCn'nnnn_
Cuts at: 599 599
Size: 4523
Bsp1286I G_dGCh'C
Cuts at: 204 239 398 638 866 952 1063 1103 1124
Size: 35 159 240 228 86 111 40 21
Cuts at: 1124 1334 1358 1430 1469 1514 1537 2148 2241
Size: 210 24 72 39 45 23 611 93
Cuts at: 2241 2315 2505 2567 3297 204
Size: 74 190 62 730 1430
Fragments arranged by size:
1430 730 611 240 228 210 190 159
111 93 86 74 72 62 45 40
39 35 24 23 21
BspEI T'CCGG_A
Cuts at: 467 3743 467
Size: 3276 1247
BspHI T'CATG_A
Cuts at: 3699 3699
Size: 4523
BspMI ACCTGCnnnn'nnnn_
Cuts at: 738 827 1041 1573 1787 2089 2470 738
Size: 89 214 532 214 302 381 2791
Fragments arranged by size:
2791 532 381 302 214 214 89
BsrBI CCG'CTC
Cuts at: 50 115 531 645 2815 2869 4353 4518 50
Size: 65 416 114 2170 54 1484 165 55
Fragments arranged by size:
2170 1484 416 165 114 65 55 54
BsrDI GCAATG_nn'
Cuts at: 884 2435 884
Size: 1551 2972
BsrFI r'CCGG_y
Cuts at: 196 245 797 2521 2702 2911 196
Size: 49 552 1724 181 209 1808
Fragments arranged by size:
1808 1724 552 209 181 49
BssSI C'ACGA_G
Cuts at: 619 1250 2794 3152 619
Size: 631 1544 358 1990
Fragments arranged by size:
1990 1544 631 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1900 1900
Size: 4523
BstBI TT'CG_AA
Cuts at: 2884 2884
Size: 4523
BstEII G'GTnAC_C
Cuts at: 750 750
Size: 4523
BstXI CCAn_nnnn'nTGG
Cuts at: 736 736
Size: 4523
BstYI r'GATC_y
Cuts at: 1 369 1548 2015 2038 2373 2619 3631 3717
Size: 368 1179 467 23 335 246 1012 86
Cuts at: 3717 3729 4075 1
Size: 12 346 449
Fragments arranged by size:
1179 1012 467 449 368 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 212 305 352 400 984 1193 1280 1334 212
Size: 93 47 48 584 209 87 54 3401
Fragments arranged by size:
3401 584 209 93 87 54 48 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2473 2654 2817 2473
Size: 181 163 4179
Fragments arranged by size:
4179 181 163
BtsI GCAGTG_nn'
Cuts at: 1150 1179 1388 1766 1150
Size: 29 209 378 3907
Fragments arranged by size:
3907 378 209 29
DraI TTT'AAA
Cuts at: 1522 1522
Size: 4523
DraIII CAC_nnn'GTG
Cuts at: 624 1080 624
Size: 456 4067
DrdI GACnn_nn'nnGTC
Cuts at: 2229 3087 2229
Size: 858 3665
EaeI y'GGCC_r
Cuts at: 987 1277 2108 2282 2673 2700 4045 987
Size: 290 831 174 391 27 1345 1465
Fragments arranged by size:
1465 1345 831 391 290 174 27
EagI C'GGCC_G
Cuts at: 987 2108 987
Size: 1121 3402
EarI CTCTTCn'nnn_
Cuts at: 469 859 1008 1276 1502 2546 2756 469
Size: 390 149 268 226 1044 210 2236
Fragments arranged by size:
2236 1044 390 268 226 210 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 549 2731 3041 3187 4333 549
Size: 2182 310 146 1146 739
Fragments arranged by size:
2182 1146 739 310 146
EcoICRI GAG'CTC
Cuts at: 1535 1535
Size: 4523
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 623 1108 2348 2780 3527 3795 307
Size: 316 485 1240 432 747 268 1035
Fragments arranged by size:
1240 1035 747 485 432 316 268
EcoO109I rG'GnC_Cy
Cuts at: 332 1059 1662 332
Size: 727 603 3193
Fragments arranged by size:
3193 727 603
EcoRI G'AATT_C
Cuts at: 1527 1527
Size: 4523
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2542 2542
Size: 4523
FspI TGC'GCA
Cuts at: 976 2304 976
Size: 1328 3195
HaeII r_GCGC'y
Cuts at: 317 328 1957 2205 3227 4206 317
Size: 11 1629 248 1022 979 634
Fragments arranged by size:
1629 1022 979 634 248 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2365 2395 2397 2427 2365
Size: 30 2 30 4461
Fragments arranged by size:
4461 30 30 2
HincII GTy'rAC
Cuts at: 1562 1562
Size: 4523
KasI G'GCGC_C
Cuts at: 1953 2201 1953
Size: 248 4275
KpnI G_GTAC'C
Cuts at: 1543 1543
Size: 4523
MluI A'CGCG_T
Cuts at: 3956 3956
Size: 4523
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 542 3194 3378 542
Size: 2652 184 1687
Fragments arranged by size:
2652 1687 184
MscI TGG'CCA
Cuts at: 1279 2284 1279
Size: 1005 3518
MslI CAynn'nnrTG
Cuts at: 517 2512 2639 517
Size: 1995 127 2401
Fragments arranged by size:
2401 1995 127
MspA1I CmG'CkG
Cuts at: 140 345 354 376 402 578 986 1202 1210
Size: 205 9 22 26 176 408 216 8
Cuts at: 1210 1454 1478 1634 1948 2308 3321 3566 140
Size: 244 24 156 314 360 1013 245 1097
Fragments arranged by size:
1097 1013 408 360 314 245 244 216
205 176 156 26 24 22 9 8
NaeI GCC'GGC
Cuts at: 247 799 2704 247
Size: 552 1905 2066
Fragments arranged by size:
2066 1905 552
NarI GG'CG_CC
Cuts at: 1954 2202 1954
Size: 248 4275
NcoI C'CATG_G
Cuts at: 1193 1280 1193
Size: 87 4436
NdeI CA'TA_TG
Cuts at: 1298 1298
Size: 4523
NgoMIV G'CCGG_C
Cuts at: 245 797 2702 245
Size: 552 1905 2066
Fragments arranged by size:
2066 1905 552
NsiI A_TGCA'T
Cuts at: 1223 1223
Size: 4523
NspI r_CATG'y
Cuts at: 1576 2076 2983 1576
Size: 500 907 3116
Fragments arranged by size:
3116 907 500
PciI A'CATG_T
Cuts at: 2979 2979
Size: 4523
PmeI GTTT'AAAC
Cuts at: 1522 1522
Size: 4523
PmlI CAC'GTG
Cuts at: 477 477
Size: 4523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3662 3694 3662
Size: 32 4491
PpuMI rG'GwC_Cy
Cuts at: 332 332
Size: 4523
PspOMI G'GGCC_C
Cuts at: 1059 1059
Size: 4523
PstI C_TGCA'G
Cuts at: 210 1570 2255 210
Size: 1360 685 2478
Fragments arranged by size:
2478 1360 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4523
PvuII CAG'CTG
Cuts at: 140 1210 1478 1948 2308 140
Size: 1070 268 470 360 2355
Fragments arranged by size:
2355 1070 470 360 268
RsrII CG'GwC_CG
Cuts at: 2718 2718
Size: 4523
SacI G_AGCT'C
Cuts at: 1537 1537
Size: 4523
SacII CC_GC'GG
Cuts at: 355 403 987 355
Size: 48 584 3891
Fragments arranged by size:
3891 584 48
SalI G'TCGA_C
Cuts at: 1560 1560
Size: 4523
SanDI GG'GwC_CC
Cuts at: 332 332
Size: 4523
SapI GCTCTTCn'nnn_
Cuts at: 469 1502 2546 2756 469
Size: 1033 1044 210 2236
Fragments arranged by size:
2236 1044 1033 210
SbfI CC_TGCA'GG
Cuts at: 1570 1570
Size: 4523
ScaI AGT'ACT
Cuts at: 4040 4040
Size: 4523
SfcI C'TryA_G
Cuts at: 33 206 1007 1566 2251 3244 3435 33
Size: 173 801 559 685 993 191 1121
Fragments arranged by size:
1121 993 801 685 559 191 173
SfoI GGC'GCC
Cuts at: 1955 2203 1955
Size: 248 4275
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4523
SgrAI Cr'CCGG_yG
Cuts at: 196 196
Size: 4523
SmaI CCC'GGG
Cuts at: 388 1545 388
Size: 1157 3366
SmlI C'TyrA_G
Cuts at: 116 1678 2593 3085 3347 116
Size: 1562 915 492 262 1292
Fragments arranged by size:
1562 1292 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4523
SphI G_CATG'C
Cuts at: 1576 2076 1576
Size: 500 4023
StuI AGG'CCT
Cuts at: 454 454
Size: 4523
StyI C'CwwG_G
Cuts at: 1193 1280 1358 1657 2011 1193
Size: 87 78 299 354 3705
Fragments arranged by size:
3705 354 299 87 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 825 1206 1445 2106 2239 2698 2907 825
Size: 381 239 661 133 459 209 2441
Fragments arranged by size:
2441 661 459 381 239 209 133
TatI w'GTAC_w
Cuts at: 4038 4038
Size: 4523
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4040 4072 4040
Size: 32 4491
Tth111I GACn'n_nGTC
Cuts at: 2320 2320
Size: 4523
XbaI T'CTAG_A
Cuts at: 1554 1554
Size: 4523
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 335 1439 2018 335
Size: 1104 579 2840
Fragments arranged by size:
2840 1104 579
XmaI C'CCGG_G
Cuts at: 386 1543 386
Size: 1157 3366
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BcgI BmgBI BmtI BsaXI BsiWI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI PacI PasI
PflMI PfoI PshAI PsiI PspXI PsrI SexAI SfiI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI