Restriction map for
pF1KE3228
(Circular) (Six-base) MAPSORT of: pF1KE3228.seq Check: 1359 from: 1 to: 4673
pF1KE3228 4673 bp
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AccI GT'mk_AC
Cuts at: 1711 1711
Size: 4673
Acc65I G'GTAC_C
Cuts at: 1689 1689
Size: 4673
AclI AA'CG_TT
Cuts at: 4513 4513
Size: 4673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1030 2498 2930 3677 3945 1000
Size: 30 1468 432 747 268 1728
Fragments arranged by size:
1728 1468 747 432 268 30
AfeI AGC'GCT
Cuts at: 1213 1647 1213
Size: 434 4239
AflIII A'CryG_T
Cuts at: 1665 2025 3129 4106 1665
Size: 360 1104 977 2232
Fragments arranged by size:
2232 1104 977 360
AgeI A'CCGG_T
Cuts at: 3061 3061
Size: 4673
AleI CACnn'nnGTG
Cuts at: 307 307
Size: 4673
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1402 1434 1402
Size: 32 4641
AlwNI CAG_nnn'CTG
Cuts at: 185 978 1203 1978 3545 185
Size: 793 225 775 1567 1313
Fragments arranged by size:
1567 1313 793 775 225
ApaLI G'TGCA_C
Cuts at: 3443 3443
Size: 4673
ApoI r'AATT_y
Cuts at: 1677 4026 4282 1677
Size: 2349 256 2068
Fragments arranged by size:
2349 2068 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4673
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
AvaI C'yCGr_G
Cuts at: 242 1693 242
Size: 1451 3222
BamHI G'GATC_C
Cuts at: 1698 1698
Size: 4673
BanI G'GyrC_C
Cuts at: 150 1599 1689 2103 2351 2386 150
Size: 1449 90 414 248 35 2437
Fragments arranged by size:
2437 1449 414 248 90 35
BanII G_rGCy'C
Cuts at: 1687 2717 1687
Size: 1030 3643
BbeI G_GCGC'C
Cuts at: 2107 2355 2107
Size: 248 4425
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4639
BciVI GTATCCnnnnn_n'
Cuts at: 2571 3332 4673 2571
Size: 761 1341 2571
Fragments arranged by size:
2571 1341 761
BclI T'GATC_A
Cuts at: 743 1226 2193 743
Size: 483 967 3223
Fragments arranged by size:
3223 967 483
BglI GCCn_nnn'nGGC
Cuts at: 869 869
Size: 4673
BglII A'GATC_T
Cuts at: 143 2188 4225 143
Size: 2045 2037 591
Fragments arranged by size:
2045 2037 591
BlpI GC'TnA_GC
Cuts at: 1785 1785
Size: 4673
Bme1580I G_kGCm'C
Cuts at: 155 1604 2298 2391 3447 155
Size: 1449 694 93 1056 1381
Fragments arranged by size:
1449 1381 1056 694 93
BmgBI CAC'GTC
Cuts at: 759 759
Size: 4673
BmrI ACTGGGnnnn_n'
Cuts at: 1885 1966 2301 4445 1885
Size: 81 335 2144 2113
Fragments arranged by size:
2144 2113 335 81
BmtI G_CTAG'C
Cuts at: 1504 1504
Size: 4673
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3040 3040
Size: 4673
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 603 874 1258 1641 603
Size: 271 384 383 3635
Fragments arranged by size:
3635 384 383 271
Bpu10I CC'TnA_GC
Cuts at: 417 417
Size: 4673
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 447 1849 2728 3220 3518 447
Size: 1402 879 492 298 1602
Fragments arranged by size:
1602 1402 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4377 4377
Size: 4673
BsaAI yAC'GTr
Cuts at: 837 2026 837
Size: 1189 3484
BsaBI GATnn'nnATC
Cuts at: 6 829 2187 4224 6
Size: 823 1358 2037 455
Fragments arranged by size:
2037 1358 823 455
BsaHI Gr'CG_yC
Cuts at: 296 1088 1369 2104 2352 3054 4550 296
Size: 792 281 735 248 702 1496 419
Fragments arranged by size:
1496 792 735 702 419 281 248
BsaWI w'CCGG_w
Cuts at: 401 1862 2383 3061 3335 3482 3893 401
Size: 1461 521 678 274 147 411 1181
Fragments arranged by size:
1461 1181 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 519 982 1111 1126 1150 1336 1561 1644 519
Size: 463 129 15 24 186 225 83 3548
Fragments arranged by size:
3548 463 225 186 129 83 24 15
BseYI C'CCAG_C
Cuts at: 1579 2098 2856 3433 3929 1579
Size: 519 758 577 496 2323
Fragments arranged by size:
2323 758 577 519 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 808 859 1337 1410 157
Size: 651 51 478 73 3420
Fragments arranged by size:
3420 651 478 73 51
BsiEI CG_ry'CG
Cuts at: 104 1031 1571 2215 2261 3469 104
Size: 927 540 644 46 1208 1308
Fragments arranged by size:
1308 1208 927 644 540 46
BsiHKAI G_wGCw'C
Cuts at: 884 951 1018 1687 2465 2655 3447 884
Size: 67 67 669 778 190 792 2110
Fragments arranged by size:
2110 792 778 669 190 67 67
BsmBI CGTCTCn'nnnn_
Cuts at: 1473 1473
Size: 4673
Bsp1286I G_dGCh'C
Cuts at: 155 884 951 1018 1604 1687 2298 2391 2465
Size: 729 67 67 586 83 611 93 74
Cuts at: 2465 2655 2717 3447 155
Size: 190 62 730 1381
Fragments arranged by size:
1381 730 729 611 586 190 93 83
74 67 67 62
BspEI T'CCGG_A
Cuts at: 401 3893 401
Size: 3492 1181
BspHI T'CATG_A
Cuts at: 668 1475 3849 668
Size: 807 2374 1492
Fragments arranged by size:
2374 1492 807
BspMI ACCTGCnnnn'nnnn_
Cuts at: 995 1723 1937 2239 2620 995
Size: 728 214 302 381 3048
Fragments arranged by size:
3048 728 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1242 1473 2965 3019 4503 4668 50
Size: 1192 231 1492 54 1484 165 55
Fragments arranged by size:
1492 1484 1192 231 165 55 54
BsrDI GCAATG_nn'
Cuts at: 415 2585 415
Size: 2170 2503
BsrFI r'CCGG_y
Cuts at: 863 2671 2852 3061 863
Size: 1808 181 209 2475
Fragments arranged by size:
2475 1808 209 181
BssSI C'ACGA_G
Cuts at: 2944 3302 2944
Size: 358 4315
BstAPI GCAn_nnn'nTGC
Cuts at: 2050 2050
Size: 4673
BstBI TT'CG_AA
Cuts at: 3034 3034
Size: 4673
BstXI CCAn_nnnn'nTGG
Cuts at: 114 603 114
Size: 489 4184
BstYI r'GATC_y
Cuts at: 1 143 181 1698 2165 2188 2523 2769 3781
Size: 142 38 1517 467 23 335 246 1012
Cuts at: 3781 3867 3879 4225 1
Size: 86 12 346 449
Fragments arranged by size:
1517 1012 467 449 346 335 246 142
86 38 23 12
Bsu36I CC'TnA_GG
Cuts at: 1133 1133
Size: 4673
BtgI C'CryG_G
Cuts at: 155 656 155
Size: 501 4172
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2623 2804 2967 2623
Size: 181 163 4329
Fragments arranged by size:
4329 181 163
BtsI GCAGTG_nn'
Cuts at: 1063 1916 1063
Size: 853 3820
DraI TTT'AAA
Cuts at: 1672 1672
Size: 4673
DrdI GACnn_nn'nnGTC
Cuts at: 1087 2379 3237 1087
Size: 1292 858 2523
Fragments arranged by size:
2523 1292 858
EaeI y'GGCC_r
Cuts at: 1028 1568 2258 2432 2823 2850 4195 1028
Size: 540 690 174 391 27 1345 1506
Fragments arranged by size:
1506 1345 690 540 391 174 27
EagI C'GGCC_G
Cuts at: 1028 1568 2258 1028
Size: 540 690 3443
Fragments arranged by size:
3443 690 540
EarI CTCTTCn'nnn_
Cuts at: 667 1006 1352 2696 2906 667
Size: 339 346 1344 210 2434
Fragments arranged by size:
2434 1344 346 339 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 361 2881 3191 3337 4483 361
Size: 2520 310 146 1146 551
Fragments arranged by size:
2520 1146 551 310 146
EcoICRI GAG'CTC
Cuts at: 1685 1685
Size: 4673
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 603 874 1000 1030 1258 1641 2498 2930 3677
Size: 271 126 30 228 383 857 432 747
Cuts at: 3677 3945 603
Size: 268 1331
Fragments arranged by size:
1331 857 747 432 383 271 268 228
126 30
EcoO109I rG'GnC_Cy
Cuts at: 167 984 1200 1462 1812 167
Size: 817 216 262 350 3028
Fragments arranged by size:
3028 817 350 262 216
EcoRI G'AATT_C
Cuts at: 1677 1677
Size: 4673
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4673
FspI TGC'GCA
Cuts at: 2454 2454
Size: 4673
HaeII r_GCGC'y
Cuts at: 1039 1069 1215 1649 2107 2355 3377 4356 1039
Size: 30 146 434 458 248 1022 979 1356
Fragments arranged by size:
1356 1022 979 458 434 248 146 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 500 532 628 660 2515 2545 2547 2577 500
Size: 32 96 32 1855 30 2 30 2596
Fragments arranged by size:
2596 1855 96 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1712 1712
Size: 4673
KasI G'GCGC_C
Cuts at: 2103 2351 2103
Size: 248 4425
KpnI G_GTAC'C
Cuts at: 1693 1693
Size: 4673
MluI A'CGCG_T
Cuts at: 4106 4106
Size: 4673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 95 3344 3528 95
Size: 3249 184 1240
Fragments arranged by size:
3249 1240 184
MscI TGG'CCA
Cuts at: 2434 2434
Size: 4673
MslI CAynn'nnrTG
Cuts at: 223 307 835 2662 2789 223
Size: 84 528 1827 127 2107
Fragments arranged by size:
2107 1827 528 127 84
MspA1I CmG'CkG
Cuts at: 1008 1583 1611 1784 2098 2458 3471 3716 1008
Size: 575 28 173 314 360 1013 245 1965
Fragments arranged by size:
1965 1013 575 360 314 245 173 28
NaeI GCC'GGC
Cuts at: 865 2854 865
Size: 1989 2684
NarI GG'CG_CC
Cuts at: 2104 2352 2104
Size: 248 4425
NcoI C'CATG_G
Cuts at: 656 656
Size: 4673
NgoMIV G'CCGG_C
Cuts at: 863 2852 863
Size: 1989 2684
NheI G'CTAG_C
Cuts at: 1500 1500
Size: 4673
NspI r_CATG'y
Cuts at: 1555 1726 2226 3133 1555
Size: 171 500 907 3095
Fragments arranged by size:
3095 907 500 171
PciI A'CATG_T
Cuts at: 3129 3129
Size: 4673
PflMI CCAn_nnn'nTGG
Cuts at: 1203 1215 1203
Size: 12 4661
PfoI T'CCnGG_A
Cuts at: 1304 1636 1657 1304
Size: 332 21 4320
Fragments arranged by size:
4320 332 21
PmeI GTTT'AAAC
Cuts at: 1672 1672
Size: 4673
PmlI CAC'GTG
Cuts at: 837 837
Size: 4673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4641
PpuMI rG'GwC_Cy
Cuts at: 984 1200 1462 984
Size: 216 262 4195
Fragments arranged by size:
4195 262 216
PshAI GACnn'nnGTC
Cuts at: 1372 1372
Size: 4673
PstI C_TGCA'G
Cuts at: 1720 2405 1720
Size: 685 3988
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4673
PvuII CAG'CTG
Cuts at: 1008 1583 1611 2098 2458 1008
Size: 575 28 487 360 3223
Fragments arranged by size:
3223 575 487 360 28
RsrII CG'GwC_CG
Cuts at: 2868 2868
Size: 4673
SacI G_AGCT'C
Cuts at: 1687 1687
Size: 4673
SalI G'TCGA_C
Cuts at: 1710 1710
Size: 4673
SapI GCTCTTCn'nnn_
Cuts at: 1006 2696 2906 1006
Size: 1690 210 2773
Fragments arranged by size:
2773 1690 210
SbfI CC_TGCA'GG
Cuts at: 1720 1720
Size: 4673
ScaI AGT'ACT
Cuts at: 1165 4190 1165
Size: 3025 1648
SexAI A'CCwGG_T
Cuts at: 1202 1202
Size: 4673
SfcI C'TryA_G
Cuts at: 33 581 1716 2401 3394 3585 33
Size: 548 1135 685 993 191 1121
Fragments arranged by size:
1135 1121 993 685 548 191
SfoI GGC'GCC
Cuts at: 2105 2353 2105
Size: 248 4425
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
SmaI CCC'GGG
Cuts at: 244 1695 244
Size: 1451 3222
SmlI C'TyrA_G
Cuts at: 426 1828 2743 3235 3497 426
Size: 1402 915 492 262 1602
Fragments arranged by size:
1602 1402 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4673
SphI G_CATG'C
Cuts at: 1726 2226 1726
Size: 500 4173
StuI AGG'CCT
Cuts at: 917 917
Size: 4673
StyI C'CwwG_G
Cuts at: 656 1807 2161 656
Size: 1151 354 3168
Fragments arranged by size:
3168 1151 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 138 2256 2389 2848 3057 138
Size: 2118 133 459 209 1754
Fragments arranged by size:
2118 1754 459 209 133
TatI w'GTAC_w
Cuts at: 350 815 1163 1254 4188 350
Size: 465 348 91 2934 835
Fragments arranged by size:
2934 835 465 348 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 396 428 4190 4222 396
Size: 32 3762 32 847
Fragments arranged by size:
3762 847 32 32
Tth111I GACn'n_nGTC
Cuts at: 1204 2470 1204
Size: 1266 3407
XbaI T'CTAG_A
Cuts at: 1704 1704
Size: 4673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2168 2168
Size: 4673
XmaI C'CCGG_G
Cuts at: 242 1693 242
Size: 1451 3222
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI ApaI AscI AvrII
BaeI BbsI BbvCI BfrBI BsaXI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NotI NruI
NsiI PacI PasI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI