Restriction map for
pF1KE3269
(Circular) (Six-base) MAPSORT of: pF1KE3269.seq Check: 2791 from: 1 to: 5123
pF1KE3269 5123 bp
With 182 enzymes: SgfI *
February 10, 2017 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1869 1869
Size: 5123
AatII G_ACGT'C
Cuts at: 179 1124 1780 179
Size: 945 656 3522
Fragments arranged by size:
3522 945 656
AccI GT'mk_AC
Cuts at: 1319 1793 2161 1319
Size: 474 368 4281
Fragments arranged by size:
4281 474 368
Acc65I G'GTAC_C
Cuts at: 2139 2139
Size: 5123
AclI AA'CG_TT
Cuts at: 4963 4963
Size: 5123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 1326 1569 1618 1657 1941 2948 3380 4127
Size: 338 243 49 39 284 1007 432 747
Cuts at: 4127 4395 988
Size: 268 1716
Fragments arranged by size:
1716 1007 747 432 338 284 268 243
49 39
AflIII A'CryG_T
Cuts at: 541 551 1381 1853 2475 3579 4556 541
Size: 10 830 472 622 1104 977 1108
Fragments arranged by size:
1108 1104 977 830 622 472 10
AgeI A'CCGG_T
Cuts at: 1973 3511 1973
Size: 1538 3585
AhdI GACnn_n'nnGTC
Cuts at: 1200 1866 1200
Size: 666 4457
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 430 430
Size: 5123
AlwNI CAG_nnn'CTG
Cuts at: 1512 1988 2022 2086 2428 3995 1512
Size: 476 34 64 342 1567 2640
Fragments arranged by size:
2640 1567 476 342 64 34
ApaI G_GGCC'C
Cuts at: 155 1300 1539 1683 155
Size: 1145 239 144 3595
Fragments arranged by size:
3595 1145 239 144
ApaLI G'TGCA_C
Cuts at: 903 1554 3893 903
Size: 651 2339 2133
Fragments arranged by size:
2339 2133 651
ApoI r'AATT_y
Cuts at: 2127 4476 4732 2127
Size: 2349 256 2518
Fragments arranged by size:
2518 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5123
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5123
AvaI C'yCGr_G
Cuts at: 436 728 2063 2143 436
Size: 292 1335 80 3416
Fragments arranged by size:
3416 1335 292 80
AvrII C'CTAG_G
Cuts at: 1236 1236
Size: 5123
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1368 1401 1368
Size: 33 5090
BamHI G'GATC_C
Cuts at: 2148 2148
Size: 5123
BanI G'GyrC_C
Cuts at: 734 1831 1906 2139 2553 2801 2836 734
Size: 1097 75 233 414 248 35 3021
Fragments arranged by size:
3021 1097 414 248 233 75 35
BanII G_rGCy'C
Cuts at: 115 155 659 1112 1300 1539 1655 1683 2137
Size: 40 504 453 188 239 116 28 454
Cuts at: 2137 3167 115
Size: 1030 2071
Fragments arranged by size:
2071 1030 504 454 453 239 188 116
40 28
BbeI G_GCGC'C
Cuts at: 2557 2805 2557
Size: 248 4875
BbsI GAAGACnn'nnnn_
Cuts at: 510 1411 1947 510
Size: 901 536 3686
Fragments arranged by size:
3686 901 536
BbvCI CC'TCA_GC
Cuts at: 1253 1253
Size: 5123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 894 928 894
Size: 34 5089
BciVI GTATCCnnnnn_n'
Cuts at: 3021 3782 5123 3021
Size: 761 1341 3021
Fragments arranged by size:
3021 1341 761
BclI T'GATC_A
Cuts at: 533 2643 533
Size: 2110 3013
BglI GCCn_nnn'nGGC
Cuts at: 677 1295 1424 1687 1904 677
Size: 618 129 263 217 3896
Fragments arranged by size:
3896 618 263 217 129
BglII A'GATC_T
Cuts at: 1259 1733 2638 4675 1259
Size: 474 905 2037 1707
Fragments arranged by size:
2037 1707 905 474
BlpI GC'TnA_GC
Cuts at: 754 1147 2235 754
Size: 393 1088 3642
Fragments arranged by size:
3642 1088 393
Bme1580I G_kGCm'C
Cuts at: 129 155 551 739 907 1300 1539 1558 1630
Size: 26 396 188 168 393 239 19 72
Cuts at: 1630 1683 1909 2748 2841 3897 129
Size: 53 226 839 93 1056 1355
Fragments arranged by size:
1355 1056 839 396 393 239 226 188
168 93 72 53 26 19
BmgBI CAC'GTC
Cuts at: 1767 1767
Size: 5123
BmrI ACTGGGnnnn_n'
Cuts at: 1680 2335 2416 2751 4895 1680
Size: 655 81 335 2144 1908
Fragments arranged by size:
2144 1908 655 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 498 3490 498
Size: 2992 2131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 868 964 1126 1432 2010 279
Size: 589 96 162 306 578 3392
Fragments arranged by size:
3392 589 578 306 162 96
Bpu10I CC'TnA_GC
Cuts at: 114 835 1253 114
Size: 721 418 3984
Fragments arranged by size:
3984 721 418
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 1096 2299 3178 3670 3968 844
Size: 252 1203 879 492 298 1999
Fragments arranged by size:
1999 1203 879 492 298 252
BsaI GGTCTCn'nnnn_
Cuts at: 928 1569 4827 928
Size: 641 3258 1224
Fragments arranged by size:
3258 1224 641
BsaAI yAC'GTr
Cuts at: 542 1014 1854 1902 2476 542
Size: 472 840 48 574 3189
Fragments arranged by size:
3189 840 574 472 48
BsaBI GATnn'nnATC
Cuts at: 6 2637 4674 6
Size: 2631 2037 455
Fragments arranged by size:
2631 2037 455
BsaHI Gr'CG_yC
Cuts at: 176 1121 1777 1936 2554 2802 3504 5000 176
Size: 945 656 159 618 248 702 1496 299
Fragments arranged by size:
1496 945 702 656 618 299 248 159
BsaWI w'CCGG_w
Cuts at: 1973 2312 2833 3511 3785 3932 4343 1973
Size: 339 521 678 274 147 411 2753
Fragments arranged by size:
2753 678 521 411 339 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 127 157 1099 1129 1699 1729 127
Size: 30 942 30 570 30 3521
Fragments arranged by size:
3521 942 570 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 279 282 340 454 457 460 463 1341 1557
Size: 3 58 114 3 3 3 878 216
Cuts at: 1557 279
Size: 3845
Fragments arranged by size:
3845 878 216 114 58 3 3 3
3
BseYI C'CCAG_C
Cuts at: 154 817 1062 2548 3306 3883 4379 154
Size: 663 245 1486 758 577 496 898
Fragments arranged by size:
1486 898 758 663 577 496 245
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 1467 1995 376
Size: 1091 528 3504
Fragments arranged by size:
3504 1091 528
BsiEI CG_ry'CG
Cuts at: 104 1974 2665 2711 3919 104
Size: 1870 691 46 1208 1308
Fragments arranged by size:
1870 1308 1208 691 46
BsiHKAI G_wGCw'C
Cuts at: 115 659 875 907 1112 1558 1583 1655 2137
Size: 544 216 32 205 446 25 72 482
Cuts at: 2137 2915 3105 3897 115
Size: 778 190 792 1341
Fragments arranged by size:
1341 792 778 544 482 446 216 205
190 72 32 25
Bsp1286I G_dGCh'C
Cuts at: 115 129 155 551 659 739 875 907 1112
Size: 14 26 396 108 80 136 32 205
Cuts at: 1112 1300 1539 1558 1583 1630 1655 1683 1909
Size: 188 239 19 25 47 25 28 226
Cuts at: 1909 2137 2748 2841 2915 3105 3167 3897 115
Size: 228 611 93 74 190 62 730 1341
Fragments arranged by size:
1341 730 611 396 239 228 226 205
190 188 136 108 93 80 74 62
47 32 28 26 25 25 19 14
BspEI T'CCGG_A
Cuts at: 4343 4343
Size: 5123
BspHI T'CATG_A
Cuts at: 4299 4299
Size: 5123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1343 1520 1869 2173 2387 2689 3070 1343
Size: 177 349 304 214 302 381 3396
Fragments arranged by size:
3396 381 349 304 302 214 177
BsrBI CCG'CTC
Cuts at: 50 362 386 3415 3469 4953 5118 50
Size: 312 24 3029 54 1484 165 55
Fragments arranged by size:
3029 1484 312 165 55 54 24
BsrDI GCAATG_nn'
Cuts at: 1799 3035 1799
Size: 1236 3887
BsrFI r'CCGG_y
Cuts at: 671 1973 3121 3302 3511 671
Size: 1302 1148 181 209 2283
Fragments arranged by size:
2283 1302 1148 209 181
BsrGI T'GTAC_A
Cuts at: 1706 1706
Size: 5123
BssSI C'ACGA_G
Cuts at: 772 1003 3394 3752 772
Size: 231 2391 358 2143
Fragments arranged by size:
2391 2143 358 231
BstAPI GCAn_nnn'nTGC
Cuts at: 2022 2500 2022
Size: 478 4645
BstBI TT'CG_AA
Cuts at: 3484 3484
Size: 5123
BstEII G'GTnAC_C
Cuts at: 1330 1330
Size: 5123
BstYI r'GATC_y
Cuts at: 1 1259 1733 2148 2615 2638 2973 3219 4231
Size: 1258 474 415 467 23 335 246 1012
Cuts at: 4231 4317 4329 4675 1
Size: 86 12 346 449
Fragments arranged by size:
1258 1012 474 467 449 415 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1981 1981
Size: 5123
BtgI C'CryG_G
Cuts at: 316 593 614 1325 316
Size: 277 21 711 4114
Fragments arranged by size:
4114 711 277 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 615 3073 3254 3417 615
Size: 2458 181 163 2321
Fragments arranged by size:
2458 2321 181 163
BtsI GCAGTG_nn'
Cuts at: 273 430 666 2046 2366 273
Size: 157 236 1380 320 3030
Fragments arranged by size:
3030 1380 320 236 157
DraI TTT'AAA
Cuts at: 2122 2122
Size: 5123
DraIII CAC_nnn'GTG
Cuts at: 542 1578 1674 542
Size: 1036 96 3991
Fragments arranged by size:
3991 1036 96
DrdI GACnn_nn'nnGTC
Cuts at: 2829 3687 2829
Size: 858 4265
EaeI y'GGCC_r
Cuts at: 638 2708 2882 3273 3300 4645 638
Size: 2070 174 391 27 1345 1116
Fragments arranged by size:
2070 1345 1116 391 174 27
EagI C'GGCC_G
Cuts at: 2708 2708
Size: 5123
EarI CTCTTCn'nnn_
Cuts at: 664 1093 1345 1588 2080 3146 3356 664
Size: 429 252 243 492 1066 210 2431
Fragments arranged by size:
2431 1066 492 429 252 243 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 360 3331 3641 3787 4933 360
Size: 2971 310 146 1146 550
Fragments arranged by size:
2971 1146 550 310 146
EcoICRI GAG'CTC
Cuts at: 113 657 1110 1653 2135 113
Size: 544 453 543 482 3101
Fragments arranged by size:
3101 544 543 482 453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 868 964 988 1126 1326 1432 1569 1618
Size: 589 96 24 138 200 106 137 49
Cuts at: 1618 1657 1941 2010 2948 3380 4127 4395 279
Size: 39 284 69 938 432 747 268 1007
Fragments arranged by size:
1007 938 747 589 432 284 268 200
138 137 106 96 69 49 39 24
EcoNI CCTnn'n_nnAGG
Cuts at: 1516 1516
Size: 5123
EcoO109I rG'GnC_Cy
Cuts at: 151 963 1155 1277 1296 1297 1680 2262 151
Size: 812 192 122 19 1 383 582 3012
Fragments arranged by size:
3012 812 582 383 192 122 19 1
EcoRI G'AATT_C
Cuts at: 2127 2127
Size: 5123
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3142 3142
Size: 5123
FspI TGC'GCA
Cuts at: 2904 2904
Size: 5123
HaeII r_GCGC'y
Cuts at: 761 2557 2805 3827 4806 761
Size: 1796 248 1022 979 1078
Fragments arranged by size:
1796 1078 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1186 1218 1852 1884 2965 2995 2997 3027 1186
Size: 32 634 32 1081 30 2 30 3282
Fragments arranged by size:
3282 1081 634 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2162 2162
Size: 5123
KasI G'GCGC_C
Cuts at: 2553 2801 2553
Size: 248 4875
KpnI G_GTAC'C
Cuts at: 2143 2143
Size: 5123
MluI A'CGCG_T
Cuts at: 4556 4556
Size: 5123
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 389 3794 3978 389
Size: 3405 184 1534
Fragments arranged by size:
3405 1534 184
MscI TGG'CCA
Cuts at: 2884 2884
Size: 5123
MslI CAynn'nnrTG
Cuts at: 634 1369 1492 1804 1852 3112 3239 634
Size: 735 123 312 48 1260 127 2518
Fragments arranged by size:
2518 1260 735 312 127 123 48
MspA1I CmG'CkG
Cuts at: 318 698 782 821 831 1469 1589 1712 2234
Size: 380 84 39 10 638 120 123 522
Cuts at: 2234 2548 2908 3921 4166 318
Size: 314 360 1013 245 1275
Fragments arranged by size:
1275 1013 638 522 380 360 314 245
123 120 84 39 10
NaeI GCC'GGC
Cuts at: 673 3304 673
Size: 2631 2492
NarI GG'CG_CC
Cuts at: 2554 2802 2554
Size: 248 4875
NcoI C'CATG_G
Cuts at: 1325 1325
Size: 5123
NgoMIV G'CCGG_C
Cuts at: 671 3302 671
Size: 2631 2492
NspI r_CATG'y
Cuts at: 339 411 1385 2176 2676 3583 339
Size: 72 974 791 500 907 1879
Fragments arranged by size:
1879 974 907 791 500 72
PciI A'CATG_T
Cuts at: 1381 3579 1381
Size: 2198 2925
PfoI T'CCnGG_A
Cuts at: 1124 1124
Size: 5123
PmeI GTTT'AAAC
Cuts at: 2122 2122
Size: 5123
PmlI CAC'GTG
Cuts at: 542 1014 1854 1902 542
Size: 472 840 48 3763
Fragments arranged by size:
3763 840 472 48
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4262 4294 4262
Size: 32 5091
PpuMI rG'GwC_Cy
Cuts at: 1155 1277 1155
Size: 122 5001
PshAI GACnn'nnGTC
Cuts at: 1951 1951
Size: 5123
PspOMI G'GGCC_C
Cuts at: 151 1296 1535 1679 151
Size: 1145 239 144 3595
Fragments arranged by size:
3595 1145 239 144
PstI C_TGCA'G
Cuts at: 280 514 983 1479 1517 1775 2170 2855 280
Size: 234 469 496 38 258 395 685 2548
Fragments arranged by size:
2548 685 496 469 395 258 234 38
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5123
PvuII CAG'CTG
Cuts at: 782 821 2548 2908 782
Size: 39 1727 360 2997
Fragments arranged by size:
2997 1727 360 39
RsrII CG'GwC_CG
Cuts at: 3318 3318
Size: 5123
SacI G_AGCT'C
Cuts at: 115 659 1112 1655 2137 115
Size: 544 453 543 482 3101
Fragments arranged by size:
3101 544 543 482 453
SacII CC_GC'GG
Cuts at: 319 319
Size: 5123
SalI G'TCGA_C
Cuts at: 2160 2160
Size: 5123
SapI GCTCTTCn'nnn_
Cuts at: 664 1093 1345 1588 3146 3356 664
Size: 429 252 243 1558 210 2431
Fragments arranged by size:
2431 1558 429 252 243 210
SbfI CC_TGCA'GG
Cuts at: 2170 2170
Size: 5123
ScaI AGT'ACT
Cuts at: 4640 4640
Size: 5123
SfcI C'TryA_G
Cuts at: 33 276 510 558 789 979 1464 1475 1513
Size: 243 234 48 231 190 485 11 38
Cuts at: 1513 1771 2166 2851 3844 4035 33
Size: 258 395 685 993 191 1121
Fragments arranged by size:
1121 993 685 485 395 258 243 234
231 191 190 48 38 11
SfiI GGCCn_nnn'nGGCC
Cuts at: 1295 1295
Size: 5123
SfoI GGC'GCC
Cuts at: 2555 2803 2555
Size: 248 4875
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5123
SmaI CCC'GGG
Cuts at: 2065 2145 2065
Size: 80 5043
SmlI C'TyrA_G
Cuts at: 859 1111 2278 3193 3685 3947 859
Size: 252 1167 915 492 262 2035
Fragments arranged by size:
2035 1167 915 492 262 252
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5123
SphI G_CATG'C
Cuts at: 339 2176 2676 339
Size: 1837 500 2786
Fragments arranged by size:
2786 1837 500
StuI AGG'CCT
Cuts at: 795 1661 795
Size: 866 4257
StyI C'CwwG_G
Cuts at: 1236 1325 1871 2257 2611 1236
Size: 89 546 386 354 3748
Fragments arranged by size:
3748 546 386 354 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1318 1662 2706 2839 3298 3507 1318
Size: 344 1044 133 459 209 2934
Fragments arranged by size:
2934 1044 459 344 209 133
TatI w'GTAC_w
Cuts at: 1706 4638 1706
Size: 2932 2191
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 385 417 4640 4672 385
Size: 32 4223 32 836
Fragments arranged by size:
4223 836 32 32
Tth111I GACn'n_nGTC
Cuts at: 601 2920 601
Size: 2319 2804
XbaI T'CTAG_A
Cuts at: 2154 2154
Size: 5123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 717 1825 2618 717
Size: 1108 793 3222
Fragments arranged by size:
3222 1108 793
XmaI C'CCGG_G
Cuts at: 2063 2143 2063
Size: 80 5043
ZraI GAC'GTC
Cuts at: 177 1122 1778 177
Size: 945 656 3522
Fragments arranged by size:
3522 945 656
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AleI AloI AscI BfrBI BmtI BsiWI
BsmI BsmBI BssHII BstXI BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PsiI PspXI PsrI
SanDI SexAI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI