Restriction map for
pF1KE3300
(Circular) (Six-base) MAPSORT of: pF1KE3300.seq Check: 6629 from: 1 to: 10778
pF1KE3300 10778 bp
With 182 enzymes: SgfI *
May 26, 2016 12:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 463 637 697 811 1162 1312 1513 1879 1966
Size: 174 60 114 351 150 201 366 87
Cuts at: 1966 2191 2218 2251 2416 2449 3025 3106 3133
Size: 225 27 33 165 33 576 81 27
Cuts at: 3133 3253 3592 3706 3844 3877 4078 4228 6815
Size: 120 339 114 138 33 201 150 2587
Cuts at: 6815 6941 7248 463
Size: 126 307 3993
Fragments arranged by size:
3993 2587 576 366 351 339 307 225
201 201 174 165 150 150 138 126
120 114 114 87 81 60 33 33
33 27 27
AatII G_ACGT'C
Cuts at: 824 1466 5120 5729 6170 824
Size: 642 3654 609 441 5432
Fragments arranged by size:
5432 3654 642 609 441
AccI GT'mk_AC
Cuts at: 2711 5135 7816 2711
Size: 2424 2681 5673
Fragments arranged by size:
5673 2681 2424
Acc65I G'GTAC_C
Cuts at: 7794 7794
Size: 10778
AclI AA'CG_TT
Cuts at: 10618 10618
Size: 10778
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2017 2439 3015 4590 4710 5492 5518 6271 8603
Size: 422 576 1575 120 782 26 753 2332
Cuts at: 8603 9035 9782 10050 2017
Size: 432 747 268 2745
Fragments arranged by size:
2745 2332 1575 782 753 747 576 432
422 268 120 26
AfeI AGC'GCT
Cuts at: 3895 5222 3895
Size: 1327 9451
AflIII A'CryG_T
Cuts at: 2432 2521 2581 3008 4138 4517 8130 9234 10211
Size: 89 60 427 1130 379 3613 1104 977
Cuts at: 10211 2432
Size: 2999
Fragments arranged by size:
3613 2999 1130 1104 977 427 379 89
60
AgeI A'CCGG_T
Cuts at: 9166 9166
Size: 10778
AhdI GACnn_n'nnGTC
Cuts at: 3801 7665 3801
Size: 3864 6914
AleI CACnn'nnGTG
Cuts at: 833 1762 3100 3293 3838 4828 4915 5200 833
Size: 929 1338 193 545 990 87 285 6411
Fragments arranged by size:
6411 1338 990 929 545 285 193 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1225 1558 2575 5669 6016 1225
Size: 333 1017 3094 347 5987
Fragments arranged by size:
5987 3094 1017 347 333
AlwNI CAG_nnn'CTG
Cuts at: 573 1428 1601 2054 2618 3158 3371 3530 3566
Size: 855 173 453 564 540 213 159 36
Cuts at: 3566 4277 4587 5649 6045 7209 7296 7443 7551
Size: 711 310 1062 396 1164 87 147 108
Cuts at: 7551 8083 9650 573
Size: 532 1567 1701
Fragments arranged by size:
1701 1567 1164 1062 855 711 564 540
532 453 396 310 213 173 159 147
108 87 36
ApaI G_GGCC'C
Cuts at: 271 395 1327 1447 4237 4249 6987 7450 271
Size: 124 932 120 2790 12 2738 463 3599
Fragments arranged by size:
3599 2790 2738 932 463 124 120 12
ApaLI G'TGCA_C
Cuts at: 1561 1767 2436 3012 3240 3286 3754 7465 9548
Size: 206 669 576 228 46 468 3711 2083
Cuts at: 9548 1561
Size: 2791
Fragments arranged by size:
3711 2791 2083 669 576 468 228 206
46
ApoI r'AATT_y
Cuts at: 4363 6990 7782 10131 10387 4363
Size: 2627 792 2349 256 4754
Fragments arranged by size:
4754 2627 2349 792 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 10778
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10778
AvaI C'yCGr_G
Cuts at: 664 1311 2061 2221 4462 4804 6314 6865 7798
Size: 647 750 160 2241 342 1510 551 933
Cuts at: 7798 664
Size: 3644
Fragments arranged by size:
3644 2241 1510 933 750 647 551 342
160
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4140 4173 4140
Size: 33 10745
BamHI G'GATC_C
Cuts at: 3038 7803 3038
Size: 4765 6013
BanI G'GyrC_C
Cuts at: 123 451 685 799 916 1147 1363 1726 1954
Size: 328 234 114 117 231 216 363 228
Cuts at: 1954 2065 2179 2404 2575 3094 3208 3694 3832
Size: 111 114 225 171 519 114 486 138
Cuts at: 3832 3946 4010 4066 4132 5515 5857 6842 6949
Size: 114 64 56 66 1383 342 985 107
Cuts at: 6949 7062 7098 7617 7794 8208 8456 8491 123
Size: 113 36 519 177 414 248 35 2410
Fragments arranged by size:
2410 1383 985 519 519 486 414 363
342 328 248 234 231 228 225 216
177 171 138 117 114 114 114 114
113 111 107 66 64 56 36 35
BanII G_rGCy'C
Cuts at: 271 395 1261 1327 1411 1447 2737 2869 3079
Size: 124 866 66 84 36 1290 132 210
Cuts at: 3079 3470 3814 4237 4249 4322 4336 4376 4895
Size: 391 344 423 12 73 14 40 519
Cuts at: 4895 5465 5591 6489 6970 6987 7375 7450 7492
Size: 570 126 898 481 17 388 75 42
Cuts at: 7492 7792 8822 271
Size: 300 1030 2227
Fragments arranged by size:
2227 1290 1030 898 866 570 519 481
423 391 388 344 300 210 132 126
124 84 75 73 66 42 40 36
17 14 12
BbeI G_GCGC'C
Cuts at: 127 1151 4014 4136 5861 8212 8460 127
Size: 1024 2863 122 1725 2351 248 2445
Fragments arranged by size:
2863 2445 2351 1725 1024 248 122
BbsI GAAGACnn'nnnn_
Cuts at: 4914 6027 4914
Size: 1113 9665
BbvCI CC'TCA_GC
Cuts at: 298 298
Size: 10778
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 2134 2168 3684 3718 3976 4010 90
Size: 34 2010 34 1516 34 258 34 6858
Fragments arranged by size:
6858 2010 1516 258 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8676 9437 10778 8676
Size: 761 1341 8676
Fragments arranged by size:
8676 1341 761
BclI T'GATC_A
Cuts at: 8298 8298
Size: 10778
BfrBI ATG'CAT
Cuts at: 1235 4196 6084 6423 1235
Size: 2961 1888 339 5590
Fragments arranged by size:
5590 2961 1888 339
BglI GCCn_nnn'nGGC
Cuts at: 206 500 683 815 848 1031 1661 1838 1886
Size: 294 183 132 33 183 630 177 48
Cuts at: 1886 1952 2225 2450 2684 2747 2798 2912 3026
Size: 66 273 225 234 63 51 114 114
Cuts at: 3026 3203 3254 3368 3512 3740 3878 4121 4184
Size: 177 51 114 144 228 138 243 63
Cuts at: 4184 4286 6121 7394 206
Size: 102 1835 1273 3590
Fragments arranged by size:
3590 1835 1273 630 294 273 243 234
228 225 183 183 177 177 144 138
132 114 114 114 102 66 63 63
51 51 48 33
BglII A'GATC_T
Cuts at: 8293 10330 8293
Size: 2037 8741
BlpI GC'TnA_GC
Cuts at: 4894 7374 7890 4894
Size: 2480 516 7782
Fragments arranged by size:
7782 2480 516
Bme1580I G_kGCm'C
Cuts at: 271 378 395 454 770 802 1327 1447 1565
Size: 107 17 59 316 32 525 120 118
Cuts at: 1565 1657 1771 1957 2068 2182 2440 2521 2610
Size: 92 114 186 111 114 258 81 89
Cuts at: 2610 2926 3016 3244 3290 3364 3398 3697 3745
Size: 316 90 228 46 74 34 299 48
Cuts at: 3745 3758 4069 4117 4237 4249 4357 4739 5520
Size: 13 311 48 120 12 108 382 781
Cuts at: 5520 6502 6797 6845 6987 7450 7469 8403 8496
Size: 982 295 48 142 463 19 934 93
Cuts at: 8496 9552 271
Size: 1056 1497
Fragments arranged by size:
1497 1056 982 934 781 525 463 382
316 316 311 299 295 258 228 186
142 120 120 118 114 114 111 108
107 93 92 90 89 81 74 59
48 48 48 46 34 32 19 17
13 12
BmgBI CAC'GTC
Cuts at: 1344 3393 5202 6744 7434 7505 1344
Size: 2049 1809 1542 690 71 4617
Fragments arranged by size:
4617 2049 1809 1542 690 71
BmrI ACTGGGnnnn_n'
Cuts at: 1679 3339 6216 7453 7539 7990 8071 8406 10550
Size: 1660 2877 1237 86 451 81 335 2144
Cuts at: 10550 1679
Size: 1907
Fragments arranged by size:
2877 2144 1907 1660 1237 451 335 86
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 723 1494 2682 3201 3687 7731 9145 723
Size: 771 1188 519 486 4044 1414 2356
Fragments arranged by size:
4044 2356 1414 1188 771 519 486
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1914 5161 5581 5812 5971 6160 6736 6954 7701
Size: 3247 420 231 159 189 576 218 747
Cuts at: 7701 1914
Size: 4991
Fragments arranged by size:
4991 3247 747 576 420 231 218 189
159
Bpu10I CC'TnA_GC
Cuts at: 298 7111 7622 298
Size: 6813 511 3454
Fragments arranged by size:
6813 3454 511
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5473 6538 6619 7954 8833 9325 9623 5473
Size: 1065 81 1335 879 492 298 6628
Fragments arranged by size:
6628 1335 1065 879 492 298 81
BsaI GGTCTCn'nnnn_
Cuts at: 187 1903 2815 5272 5557 5887 6325 10482 187
Size: 1716 912 2457 285 330 438 4157 483
Fragments arranged by size:
4157 2457 1716 912 483 438 330 285
BsaAI yAC'GTr
Cuts at: 333 765 2433 2522 2582 3011 4139 5322 8131
Size: 432 1668 89 60 429 1128 1183 2809
Cuts at: 8131 333
Size: 2980
Fragments arranged by size:
2980 2809 1668 1183 1128 432 429 89
60
BsaBI GATnn'nnATC
Cuts at: 6 6046 8292 10329 6
Size: 6040 2246 2037 455
Fragments arranged by size:
6040 2246 2037 455
BsaHI Gr'CG_yC
Cuts at: 124 821 1148 1463 1499 1856 3938 4011 4133
Size: 697 327 315 36 357 2082 73 122
Cuts at: 4133 5033 5117 5711 5726 5858 6097 6167 7555
Size: 900 84 594 15 132 239 70 1388
Cuts at: 7555 7712 8209 8457 9159 10655 124
Size: 157 497 248 702 1496 247
Fragments arranged by size:
2082 1496 1388 900 702 697 594 497
357 327 315 248 247 239 157 132
122 84 73 70 36 15
BsaWI w'CCGG_w
Cuts at: 5576 6050 7758 7967 8488 9166 9440 9587 9998
Size: 474 1708 209 521 678 274 147 411
Cuts at: 9998 5576
Size: 6356
Fragments arranged by size:
6356 1708 678 521 474 411 274 209
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 469 499 1243 1273 1516 1546 1825 1855 1912
Size: 30 744 30 243 30 279 30 57
Cuts at: 1912 1942 2115 2145 2851 2881 3712 3742 4756
Size: 30 173 30 706 30 831 30 1014
Cuts at: 4756 4786 7561 7591 469
Size: 30 2775 30 3656
Fragments arranged by size:
3656 2775 1014 831 744 706 279 243
173 57 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2950 3291 3667 4459 4990 4993 5163 5599 5830
Size: 341 376 792 531 3 170 436 231
Cuts at: 5830 6304 7569 2950
Size: 474 1265 6159
Fragments arranged by size:
6159 1265 792 531 474 436 376 341
231 170 3
BseYI C'CCAG_C
Cuts at: 182 610 1359 1404 2611 3364 3397 3559 4526
Size: 428 749 45 1207 753 33 162 967
Cuts at: 4526 5018 5040 5232 6492 6589 6879 7213 7232
Size: 492 22 192 1260 97 290 334 19
Cuts at: 7232 7253 7450 7600 8203 8961 9538 10034 182
Size: 21 197 150 603 758 577 496 926
Fragments arranged by size:
1260 1207 967 926 758 753 749 603
577 496 492 428 334 290 197 192
162 150 97 45 33 22 21 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1284 1591 2658 2856 2970 3051 3570 3849 3982
Size: 307 1067 198 114 81 519 279 133
Cuts at: 3982 4560 4891 5185 5290 5293 5851 6187 6463
Size: 578 331 294 105 3 558 336 276
Cuts at: 6463 6586 7141 7267 7282 7456 1284
Size: 123 555 126 15 174 4606
Fragments arranged by size:
4606 1067 578 558 555 519 336 331
307 294 279 276 198 174 133 126
123 114 105 81 15 3
BsiEI CG_ry'CG
Cuts at: 104 4820 4971 5333 5831 6894 8320 8366 9574
Size: 4716 151 362 498 1063 1426 46 1208
Cuts at: 9574 104
Size: 1308
Fragments arranged by size:
4716 1426 1308 1208 1063 498 362 151
46
BsiHKAI G_wGCw'C
Cuts at: 1357 1565 1771 1930 2440 3016 3079 3244 3290
Size: 208 206 159 510 576 63 165 46
Cuts at: 3290 3556 3730 3758 4895 6248 7117 7375 7469
Size: 266 174 28 1137 1353 869 258 94
Cuts at: 7469 7628 7792 8570 8760 9552 1357
Size: 159 164 778 190 792 2583
Fragments arranged by size:
2583 1353 1137 869 792 778 576 510
266 258 208 206 190 174 165 164
159 159 94 63 46 28
BsmI GAATG_Cn'
Cuts at: 4258 5209 7683 7754 4258
Size: 951 2474 71 7282
Fragments arranged by size:
7282 2474 951 71
BsmBI CGTCTCn'nnnn_
Cuts at: 1482 5788 6928 7698 7719 1482
Size: 4306 1140 770 21 4541
Fragments arranged by size:
4541 4306 1140 770 21
Bsp1286I G_dGCh'C
Cuts at: 271 378 395 454 770 802 1261 1327 1357
Size: 107 17 59 316 32 459 66 30
Cuts at: 1357 1411 1447 1565 1657 1771 1930 1957 2068
Size: 54 36 118 92 114 159 27 111
Cuts at: 2068 2182 2440 2521 2610 2737 2869 2926 3016
Size: 114 258 81 89 127 132 57 90
Cuts at: 3016 3079 3244 3290 3364 3398 3470 3556 3697
Size: 63 165 46 74 34 72 86 141
Cuts at: 3697 3730 3745 3758 3814 4069 4117 4237 4249
Size: 33 15 13 56 255 48 120 12
Cuts at: 4249 4322 4336 4357 4376 4739 4895 5465 5520
Size: 73 14 21 19 363 156 570 55
Cuts at: 5520 5591 6248 6489 6502 6797 6845 6970 6987
Size: 71 657 241 13 295 48 125 17
Cuts at: 6987 7117 7375 7450 7469 7492 7628 7792 8403
Size: 130 258 75 19 23 136 164 611
Cuts at: 8403 8496 8570 8760 8822 9552 271
Size: 93 74 190 62 730 1497
Fragments arranged by size:
1497 730 657 611 570 459 363 316
295 258 258 255 241 190 165 164
159 156 141 136 132 130 127 125
120 118 114 114 111 107 93 92
90 89 86 81 75 74 74 73
72 71 66 63 62 59 57 56
55 54 48 48 46 36 34 33
32 30 27 23 21 19 19 17
17 15 14 13 13 12
BspEI T'CCGG_A
Cuts at: 6050 7758 9998 6050
Size: 1708 2240 6830
Fragments arranged by size:
6830 2240 1708
BspHI T'CATG_A
Cuts at: 5762 9954 5762
Size: 4192 6586
BspMI ACCTGCnnnn'nnnn_
Cuts at: 205 463 470 637 697 811 1048 1162 1312
Size: 258 7 167 60 114 237 114 150
Cuts at: 1312 1513 1879 1966 2191 2218 2251 2416 2449
Size: 201 366 87 225 27 33 165 33
Cuts at: 2449 3025 3106 3133 3253 3592 3706 3844 3877
Size: 576 81 27 120 339 114 138 33
Cuts at: 3877 4078 4168 4228 5672 6815 6941 7248 7304
Size: 201 90 60 1444 1143 126 307 56
Cuts at: 7304 7828 8042 8344 8725 205
Size: 524 214 302 381 2258
Fragments arranged by size:
2258 1444 1143 576 524 381 366 339
307 302 258 237 225 214 201 201
167 165 150 138 126 120 114 114
114 90 87 81 60 60 56 33
33 33 27 27 7
BsrBI CCG'CTC
Cuts at: 50 117 756 5760 6522 7055 9070 9124 10608
Size: 67 639 5004 762 533 2015 54 1484
Cuts at: 10608 10773 50
Size: 165 55
Fragments arranged by size:
5004 2015 1484 762 639 533 165 67
55 54
BsrDI GCAATG_nn'
Cuts at: 8690 8690
Size: 10778
BsrFI r'CCGG_y
Cuts at: 632 856 2741 2797 2840 3376 3625 4642 4819
Size: 224 1885 56 43 536 249 1017 177
Cuts at: 4819 5153 5298 5387 5627 5826 6893 8776 8957
Size: 334 145 89 240 199 1067 1883 181
Cuts at: 8957 9166 632
Size: 209 2244
Fragments arranged by size:
2244 1885 1883 1067 1017 536 334 249
240 224 209 199 181 177 145 89
56 43
BssSI C'ACGA_G
Cuts at: 164 829 9049 9407 164
Size: 665 8220 358 1535
Fragments arranged by size:
8220 1535 665 358
BstAPI GCAn_nnn'nTGC
Cuts at: 308 361 1601 1715 2054 2165 2279 2993 3158
Size: 53 1240 114 339 111 114 714 165
Cuts at: 3158 4052 4277 4295 4587 5163 6671 7296 7329
Size: 894 225 18 292 576 1508 625 33
Cuts at: 7329 8155 308
Size: 826 2931
Fragments arranged by size:
2931 1508 1240 894 826 714 625 576
339 292 225 165 114 114 111 53
33 18
BstBI TT'CG_AA
Cuts at: 9139 9139
Size: 10778
BstXI CCAn_nnnn'nTGG
Cuts at: 1509 1737 5202 6069 6811 7458 1509
Size: 228 3465 867 742 647 4829
Fragments arranged by size:
4829 3465 867 742 647 228
BstYI r'GATC_y
Cuts at: 1 3038 6041 7803 8270 8293 8628 8874 9886
Size: 3037 3003 1762 467 23 335 246 1012
Cuts at: 9886 9972 9984 10330 1
Size: 86 12 346 449
Fragments arranged by size:
3037 3003 1762 1012 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 5417 5417
Size: 10778
BtgI C'CryG_G
Cuts at: 618 678 812 1053 1143 1194 1658 1947 2286
Size: 60 134 241 90 51 464 289 339
Cuts at: 2286 2681 2769 3633 3736 3942 4076 4095 5111
Size: 395 88 864 103 206 134 19 1016
Cuts at: 5111 5121 5282 5480 5591 5678 5736 6125 6704
Size: 10 161 198 111 87 58 389 579
Cuts at: 6704 6726 618
Size: 22 4670
Fragments arranged by size:
4670 1016 864 579 464 395 389 339
289 241 206 198 161 134 134 111
103 90 88 87 60 58 51 22
19 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 189 288 6390 8728 8909 9072 189
Size: 99 6102 2338 181 163 1895
Fragments arranged by size:
6102 2338 1895 181 163 99
BtsI GCAGTG_nn'
Cuts at: 600 1563 1791 1902 2787 3495 3981 4510 4597
Size: 963 228 111 885 708 486 529 87
Cuts at: 4597 4737 5164 5185 5638 6757 8021 600
Size: 140 427 21 453 1119 1264 3357
Fragments arranged by size:
3357 1264 1119 963 885 708 529 486
453 427 228 140 111 87 21
ClaI AT'CG_AT
Cuts at: 1808 2084 2144 1808
Size: 276 60 10442
Fragments arranged by size:
10442 276 60
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 633 668 7015 7050 633
Size: 35 6347 35 4361
Fragments arranged by size:
6347 4361 35 35
DraI TTT'AAA
Cuts at: 7777 7777
Size: 10778
DraIII CAC_nnn'GTG
Cuts at: 336 3639 6031 7245 336
Size: 3303 2392 1214 3869
Fragments arranged by size:
3869 3303 2392 1214
DrdI GACnn_nn'nnGTC
Cuts at: 5731 8484 9342 5731
Size: 2753 858 7167
Fragments arranged by size:
7167 2753 858
EaeI y'GGCC_r
Cuts at: 224 564 654 858 1197 1614 3623 4265 4614
Size: 340 90 204 339 417 2009 642 349
Cuts at: 4614 4817 4941 4968 5204 5330 5400 5828 5909
Size: 203 124 27 236 126 70 428 81
Cuts at: 5909 6122 6308 6857 6891 7226 7386 8363 8537
Size: 213 186 549 34 335 160 977 174
Cuts at: 8537 8928 8955 10300 224
Size: 391 27 1345 702
Fragments arranged by size:
2009 1345 977 702 642 549 428 417
391 349 340 339 335 236 213 204
203 186 174 160 126 124 90 81
70 34 27 27
EagI C'GGCC_G
Cuts at: 4817 4968 5330 5828 6891 8363 4817
Size: 151 362 498 1063 1472 7232
Fragments arranged by size:
7232 1472 1063 498 362 151
EarI CTCTTCn'nnn_
Cuts at: 4657 5807 8801 9011 4657
Size: 1150 2994 210 6424
Fragments arranged by size:
6424 2994 1150 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5109 6186 8986 9296 9442 10588 5109
Size: 1077 2800 310 146 1146 5299
Fragments arranged by size:
5299 2800 1146 1077 310 146
EcoICRI GAG'CTC
Cuts at: 3077 4893 7373 7790 3077
Size: 1816 2480 417 6065
Fragments arranged by size:
6065 2480 1816 417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1914 2017 2439 3015 4590 4710 5161 5492 5518
Size: 103 422 576 1575 120 451 331 26
Cuts at: 5518 5581 5812 5971 6160 6271 6736 6954 7701
Size: 63 231 159 189 111 465 218 747
Cuts at: 7701 8603 9035 9782 10050 1914
Size: 902 432 747 268 2642
Fragments arranged by size:
2642 1575 902 747 747 576 465 451
432 422 331 268 231 218 189 159
120 111 103 63 26
EcoNI CCTnn'n_nnAGG
Cuts at: 2251 5470 5950 2251
Size: 3219 480 7079
Fragments arranged by size:
7079 3219 480
EcoO109I rG'GnC_Cy
Cuts at: 257 282 392 1037 1444 2011 2291 3537 4234
Size: 25 110 645 407 567 280 1246 697
Cuts at: 4234 4246 4310 5556 6144 6630 6983 6984 7105
Size: 12 64 1246 588 486 353 1 121
Cuts at: 7105 7122 7206 7447 7917 257
Size: 17 84 241 470 3118
Fragments arranged by size:
3118 1246 1246 697 645 588 567 486
470 407 353 280 241 121 110 84
64 25 17 12 1
EcoRI G'AATT_C
Cuts at: 7782 7782
Size: 10778
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4740 8797 4740
Size: 4057 6721
FspI TGC'GCA
Cuts at: 3422 4864 4987 5668 6466 8559 3422
Size: 1442 123 681 798 2093 5641
Fragments arranged by size:
5641 2093 1442 798 681 123
HaeII r_GCGC'y
Cuts at: 127 145 1133 1151 3897 4014 4136 5224 5388
Size: 18 988 18 2746 117 122 1088 164
Cuts at: 5388 5861 5946 6880 7181 8212 8460 9482 10461
Size: 473 85 934 301 1031 248 1022 979
Cuts at: 10461 127
Size: 444
Fragments arranged by size:
2746 1088 1031 1022 988 979 934 473
444 301 248 164 122 117 85 18
18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1339 1371 1442 1474 2365 2397 2479 2511 3538
Size: 32 71 32 891 32 82 32 1027
Cuts at: 3538 3570 3787 3819 3907 3939 4309 4341 6733
Size: 32 217 32 88 32 370 32 2392
Cuts at: 6733 6765 7024 7056 7651 7683 8620 8650 8652
Size: 32 259 32 595 32 937 30 2
Cuts at: 8652 8682 1339
Size: 30 3435
Fragments arranged by size:
3435 2392 1027 937 891 595 370 259
217 88 82 71 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 645 1080 1467 1575 2397 2712 3438 3795 3912
Size: 435 387 108 822 315 726 357 117
Cuts at: 3912 5709 6171 7817 645
Size: 1797 462 1646 3606
Fragments arranged by size:
3606 1797 1646 822 726 462 435 387
357 315 117 108
KasI G'GCGC_C
Cuts at: 123 1147 4010 4132 5857 8208 8456 123
Size: 1024 2863 122 1725 2351 248 2445
Fragments arranged by size:
2863 2445 2351 1725 1024 248 122
KpnI G_GTAC'C
Cuts at: 7798 7798
Size: 10778
MluI A'CGCG_T
Cuts at: 4517 10211 4517
Size: 5694 5084
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 569 1286 2408 2522 4109 5864 7712 7721 9449
Size: 717 1122 114 1587 1755 1848 9 1728
Cuts at: 9449 9633 569
Size: 184 1714
Fragments arranged by size:
1848 1755 1728 1714 1587 1122 717 184
114 9
MscI TGG'CCA
Cuts at: 566 656 5402 7228 8539 566
Size: 90 4746 1826 1311 2805
Fragments arranged by size:
4746 2805 1826 1311 90
MslI CAynn'nnrTG
Cuts at: 833 1163 1540 1762 3100 3293 3838 4828 4915
Size: 330 377 222 1338 193 545 990 87
Cuts at: 4915 5200 5320 5651 7732 8767 8894 833
Size: 285 120 331 2081 1035 127 2717
Fragments arranged by size:
2717 2081 1338 1035 990 545 377 331
330 285 222 193 127 120 87
MspA1I CmG'CkG
Cuts at: 371 491 716 953 1008 1619 2216 2459 2783
Size: 120 225 237 55 611 597 243 324
Cuts at: 2783 3035 3353 3368 3503 3863 4016 4097 4437
Size: 252 318 15 135 360 153 81 340
Cuts at: 4437 4860 5123 5307 5659 5738 5780 6000 6477
Size: 423 263 184 352 79 42 220 477
Cuts at: 6477 6492 6977 7155 7385 7889 8203 8563 9576
Size: 15 485 178 230 504 314 360 1013
Cuts at: 9576 9821 371
Size: 245 1328
Fragments arranged by size:
1328 1013 611 597 504 485 477 423
360 360 352 340 324 318 314 263
252 245 243 237 230 225 220 184
178 153 135 120 81 79 55 42
15 15
NaeI GCC'GGC
Cuts at: 634 2743 2799 2842 3627 4644 4821 5300 5389
Size: 2109 56 43 785 1017 177 479 89
Cuts at: 5389 5828 6895 8959 634
Size: 439 1067 2064 2453
Fragments arranged by size:
2453 2109 2064 1067 1017 785 479 439
177 89 56 43
NarI GG'CG_CC
Cuts at: 124 1148 4011 4133 5858 8209 8457 124
Size: 1024 2863 122 1725 2351 248 2445
Fragments arranged by size:
2863 2445 2351 1725 1024 248 122
NcoI C'CATG_G
Cuts at: 618 1194 5111 5678 6726 618
Size: 576 3917 567 1048 4670
Fragments arranged by size:
4670 3917 1048 576 567
NdeI CA'TA_TG
Cuts at: 6383 6383
Size: 10778
NgoMIV G'CCGG_C
Cuts at: 632 2741 2797 2840 3625 4642 4819 5298 5387
Size: 2109 56 43 785 1017 177 479 89
Cuts at: 5387 5826 6893 8957 632
Size: 439 1067 2064 2453
Fragments arranged by size:
2453 2109 2064 1067 1017 785 479 439
177 89 56 43
NotI GC'GGCC_GC
Cuts at: 5330 5330
Size: 10778
NsiI A_TGCA'T
Cuts at: 1237 4198 6086 6425 1237
Size: 2961 1888 339 5590
Fragments arranged by size:
5590 2961 1888 339
NspI r_CATG'y
Cuts at: 329 1235 1384 1545 2130 4196 4801 5673 6007
Size: 906 149 161 585 2066 605 872 334
Cuts at: 6007 6084 6138 6423 6696 7737 7831 8331 9238
Size: 77 54 285 273 1041 94 500 907
Cuts at: 9238 329
Size: 1869
Fragments arranged by size:
2066 1869 1041 907 906 872 605 585
500 334 285 273 161 149 94 77
54
PasI CC'CwG_GG
Cuts at: 378 2335 3252 5697 378
Size: 1957 917 2445 5459
Fragments arranged by size:
5459 2445 1957 917
PciI A'CATG_T
Cuts at: 9234 9234
Size: 10778
PflMI CCAn_nnn'nTGG
Cuts at: 336 1344 2687 3371 4389 336
Size: 1008 1343 684 1018 6725
Fragments arranged by size:
6725 1343 1018 1008 684
PfoI T'CCnGG_A
Cuts at: 891 3805 4388 5975 891
Size: 2914 583 1587 5694
Fragments arranged by size:
5694 2914 1587 583
PmeI GTTT'AAAC
Cuts at: 7777 7777
Size: 10778
PmlI CAC'GTG
Cuts at: 333 2522 2582 3011 4139 333
Size: 2189 60 429 1128 6972
Fragments arranged by size:
6972 2189 1128 429 60
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9917 9949 9917
Size: 32 10746
PpuMI rG'GwC_Cy
Cuts at: 282 1037 2011 3537 4310 5556 6144 6630 282
Size: 755 974 1526 773 1246 588 486 4430
Fragments arranged by size:
4430 1526 1246 974 773 755 588 486
PshAI GACnn'nnGTC
Cuts at: 887 887
Size: 10778
PspOMI G'GGCC_C
Cuts at: 267 391 1323 1443 4233 4245 6983 7446 267
Size: 124 932 120 2790 12 2738 463 3599
Fragments arranged by size:
3599 2790 2738 932 463 124 120 12
PstI C_TGCA'G
Cuts at: 370 775 1105 1336 1433 2623 4498 4505 4592
Size: 405 330 231 97 1190 1875 7 87
Cuts at: 4592 5779 7052 7136 7301 7334 7825 8510 370
Size: 1187 1273 84 165 33 491 685 2638
Fragments arranged by size:
2638 1875 1273 1190 1187 685 491 405
330 231 165 97 87 84 33 7
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10778
PvuII CAG'CTG
Cuts at: 371 1008 3863 4860 5307 6477 8203 8563 371
Size: 637 2855 997 447 1170 1726 360 2586
Fragments arranged by size:
2855 2586 1726 1170 997 637 447 360
RsrII CG'GwC_CG
Cuts at: 8973 8973
Size: 10778
SacI G_AGCT'C
Cuts at: 3079 4895 7375 7792 3079
Size: 1816 2480 417 6065
Fragments arranged by size:
6065 2480 1816 417
SacII CC_GC'GG
Cuts at: 4098 5124 5739 4098
Size: 1026 615 9137
Fragments arranged by size:
9137 1026 615
SalI G'TCGA_C
Cuts at: 2710 7815 2710
Size: 5105 5673
SapI GCTCTTCn'nnn_
Cuts at: 8801 9011 8801
Size: 210 10568
SbfI CC_TGCA'GG
Cuts at: 7825 7825
Size: 10778
ScaI AGT'ACT
Cuts at: 979 4603 4711 10295 979
Size: 3624 108 5584 1462
Fragments arranged by size:
5584 3624 1462 108
SexAI A'CCwGG_T
Cuts at: 3539 3845 6458 7241 3539
Size: 306 2613 783 7076
Fragments arranged by size:
7076 2613 783 306
SfcI C'TryA_G
Cuts at: 33 366 771 1101 1332 1429 2619 2802 3234
Size: 333 405 330 231 97 1190 183 432
Cuts at: 3234 3858 4395 4494 4501 4588 5775 7048 7132
Size: 624 537 99 7 87 1187 1273 84
Cuts at: 7132 7297 7330 7612 7821 8506 9499 9690 33
Size: 165 33 282 209 685 993 191 1121
Fragments arranged by size:
1273 1190 1187 1121 993 685 624 537
432 405 333 330 282 231 209 191
183 165 99 97 87 84 33 7
SfiI GGCCn_nnn'nGGCC
Cuts at: 7394 7394
Size: 10778
SfoI GGC'GCC
Cuts at: 125 1149 4012 4134 5859 8210 8458 125
Size: 1024 2863 122 1725 2351 248 2445
Fragments arranged by size:
2863 2445 2351 1725 1024 248 122
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10778
SgrAI Cr'CCGG_yG
Cuts at: 5298 5387 5298
Size: 89 10689
SmaI CCC'GGG
Cuts at: 666 6316 7800 666
Size: 5650 1484 3644
Fragments arranged by size:
5650 3644 1484
SmlI C'TyrA_G
Cuts at: 5488 6553 6634 7933 8848 9340 9602 5488
Size: 1065 81 1299 915 492 262 6664
Fragments arranged by size:
6664 1299 1065 915 492 262 81
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10778
SphI G_CATG'C
Cuts at: 1235 1384 1545 6007 6084 6138 6423 6696 7737
Size: 149 161 4462 77 54 285 273 1041
Cuts at: 7737 7831 8331 1235
Size: 94 500 3682
Fragments arranged by size:
4462 3682 1041 500 285 273 161 149
94 77 54
SrfI GCCC'GGGC
Cuts at: 666 666
Size: 10778
StuI AGG'CCT
Cuts at: 241 586 5056 5170 6602 7765 241
Size: 345 4470 114 1432 1163 3254
Fragments arranged by size:
4470 3254 1432 1163 345 114
StyI C'CwwG_G
Cuts at: 618 1194 5111 5678 6338 6626 6726 7362 7912
Size: 576 3917 567 660 288 100 636 550
Cuts at: 7912 8266 618
Size: 354 3130
Fragments arranged by size:
3917 3130 660 636 576 567 550 354
288 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 624 748 841 1066 1351 2327 2782 2839 3358
Size: 124 93 225 285 976 455 57 519
Cuts at: 3358 3573 3877 3964 6841 6871 7246 8361 8494
Size: 215 304 87 2877 30 375 1115 133
Cuts at: 8494 8953 9162 624
Size: 459 209 2240
Fragments arranged by size:
2877 2240 1115 976 519 459 455 375
304 285 225 215 209 133 124 93
87 57 30
TatI w'GTAC_w
Cuts at: 479 977 4601 4709 6452 10293 479
Size: 498 3624 108 1743 3841 964
Fragments arranged by size:
3841 3624 1743 964 498 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 469 501 599 631 1516 1548 1825 1857 2340
Size: 32 98 32 885 32 277 32 483
Cuts at: 2340 2372 2737 2769 3030 3062 4672 4704 4756
Size: 32 365 32 261 32 1610 32 52
Cuts at: 4756 4788 7561 7593 10295 10327 469
Size: 32 2773 32 2702 32 920
Fragments arranged by size:
2773 2702 1610 920 885 483 365 277
261 98 52 32 32 32 32 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1855 8575 1855
Size: 6720 4058
XbaI T'CTAG_A
Cuts at: 7809 7809
Size: 10778
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2586 3259 5136 5946 6345 6540 6867 6960 7221
Size: 673 1877 810 399 195 327 93 261
Cuts at: 7221 7389 8273 2586
Size: 168 884 5091
Fragments arranged by size:
5091 1877 884 810 673 399 327 261
195 168 93
XmaI C'CCGG_G
Cuts at: 664 6314 7798 664
Size: 5650 1484 3644
Fragments arranged by size:
5650 3644 1484
XmnI GAAnn'nnTTC
Cuts at: 4603 4603
Size: 10778
ZraI GAC'GTC
Cuts at: 822 1464 5118 5727 6168 822
Size: 642 3654 609 441 5432
Fragments arranged by size:
5432 3654 642 609 441
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AloI AscI AvrII BmtI BsiWI BsrGI BssHII
BstEII BstZ17I EcoRV FseI FspAI HindIII HpaI MfeI
NheI NruI PacI PsiI PspXI PsrI SanDI SnaBI
SspI SwaI XhoI