Restriction map for
pF1KE3316
(Circular) (Six-base) MAPSORT of: pF1KE3316.seq Check: 2717 from: 1 to: 5747
pF1KE3316 5747 bp
With 182 enzymes: SgfI *
September 29, 2011 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2463 2463
Size: 5747
AccI GT'mk_AC
Cuts at: 844 1751 2785 844
Size: 907 1034 3806
Fragments arranged by size:
3806 1034 907
Acc65I G'GTAC_C
Cuts at: 2763 2763
Size: 5747
AclI AA'CG_TT
Cuts at: 5587 5587
Size: 5747
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 636 785 1455 1630 3572 4004 4751 5019 636
Size: 149 670 175 1942 432 747 268 1364
Fragments arranged by size:
1942 1364 747 670 432 268 175 149
AfeI AGC'GCT
Cuts at: 643 643
Size: 5747
AflIII A'CryG_T
Cuts at: 2006 2144 2306 3099 4203 5180 2006
Size: 138 162 793 1104 977 2573
Fragments arranged by size:
2573 1104 977 793 162 138
AgeI A'CCGG_T
Cuts at: 4135 4135
Size: 5747
AhdI GACnn_n'nnGTC
Cuts at: 408 2310 408
Size: 1902 3845
AleI CACnn'nnGTG
Cuts at: 1190 1190
Size: 5747
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1318 1318
Size: 5747
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 331 363 1939 1971 331
Size: 32 1576 32 4107
Fragments arranged by size:
4107 1576 32 32
AlwNI CAG_nnn'CTG
Cuts at: 984 1629 1920 2082 2423 2718 3052 4619 984
Size: 645 291 162 341 295 334 1567 2112
Fragments arranged by size:
2112 1567 645 341 334 295 291 162
ApaI G_GGCC'C
Cuts at: 681 681
Size: 5747
ApaLI G'TGCA_C
Cuts at: 535 4517 535
Size: 3982 1765
ApoI r'AATT_y
Cuts at: 1427 2751 5100 5356 1427
Size: 1324 2349 256 1818
Fragments arranged by size:
2349 1818 1324 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5747
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5747
AvaI C'yCGr_G
Cuts at: 151 228 1598 1930 2266 2767 151
Size: 77 1370 332 336 501 3131
Fragments arranged by size:
3131 1370 501 336 332 77
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2518 2551 2518
Size: 33 5714
BamHI G'GATC_C
Cuts at: 2772 2772
Size: 5747
BanI G'GyrC_C
Cuts at: 549 689 736 894 1364 1489 2262 2347 2763
Size: 140 47 158 470 125 773 85 416
Cuts at: 2763 3177 3425 3460 549
Size: 414 248 35 2836
Fragments arranged by size:
2836 773 470 416 414 248 158 140
125 85 47 35
BanII G_rGCy'C
Cuts at: 140 681 1231 1783 2761 3791 140
Size: 541 550 552 978 1030 2096
Fragments arranged by size:
2096 1030 978 552 550 541
BbeI G_GCGC'C
Cuts at: 553 693 1368 3181 3429 553
Size: 140 675 1813 248 2871
Fragments arranged by size:
2871 1813 675 248 140
BbsI GAAGACnn'nnnn_
Cuts at: 1828 1828
Size: 5747
BbvCI CC'TCA_GC
Cuts at: 1589 2341 1589
Size: 752 4995
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 526 560 526
Size: 34 5713
BciVI GTATCCnnnnn_n'
Cuts at: 3645 4406 5747 3645
Size: 761 1341 3645
Fragments arranged by size:
3645 1341 761
BclI T'GATC_A
Cuts at: 564 2324 3267 564
Size: 1760 943 3044
Fragments arranged by size:
3044 1760 943
BglI GCCn_nnn'nGGC
Cuts at: 1578 2260 2345 1578
Size: 682 85 4980
Fragments arranged by size:
4980 682 85
BglII A'GATC_T
Cuts at: 1757 3262 5299 1757
Size: 1505 2037 2205
Fragments arranged by size:
2205 2037 1505
BlpI GC'TnA_GC
Cuts at: 772 2859 772
Size: 2087 3660
Bme1580I G_kGCm'C
Cuts at: 539 681 1492 2248 3372 3465 4521 539
Size: 142 811 756 1124 93 1056 1765
Fragments arranged by size:
1765 1124 1056 811 756 142 93
BmgBI CAC'GTC
Cuts at: 410 704 1836 2543 410
Size: 294 1132 707 3614
Fragments arranged by size:
3614 1132 707 294
BmrI ACTGGGnnnn_n'
Cuts at: 2959 3040 3375 5519 2959
Size: 81 335 2144 3187
Fragments arranged by size:
3187 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4114 4114
Size: 5747
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1966 2445 2462 2584 1966
Size: 479 17 122 5129
Fragments arranged by size:
5129 479 122 17
Bpu10I CC'TnA_GC
Cuts at: 1589 1782 2048 2341 1589
Size: 193 266 293 4995
Fragments arranged by size:
4995 293 266 193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 946 2923 3802 4294 4592 592
Size: 354 1977 879 492 298 1747
Fragments arranged by size:
1977 1747 879 492 354 298
BsaI GGTCTCn'nnnn_
Cuts at: 167 246 5451 167
Size: 79 5205 463
Fragments arranged by size:
5205 463 79
BsaAI yAC'GTr
Cuts at: 3100 3100
Size: 5747
BsaBI GATnn'nnATC
Cuts at: 6 3261 5298 6
Size: 3255 2037 455
Fragments arranged by size:
3255 2037 455
BsaHI Gr'CG_yC
Cuts at: 550 690 1365 1963 3178 3426 4128 5624 550
Size: 140 675 598 1215 248 702 1496 673
Fragments arranged by size:
1496 1215 702 675 673 598 248 140
BsaWI w'CCGG_w
Cuts at: 442 1147 1817 1892 2936 3457 4135 4409 4556
Size: 705 670 75 1044 521 678 274 147
Cuts at: 4556 4967 442
Size: 411 1222
Fragments arranged by size:
1222 1044 705 678 670 521 411 274
147 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1939 1969 1939
Size: 30 5717
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 155 816 949 1102 1210 1213 155
Size: 661 133 153 108 3 4689
Fragments arranged by size:
4689 661 153 133 108 3
BseYI C'CCAG_C
Cuts at: 1604 1657 1776 2615 3172 3930 4507 5003 1604
Size: 53 119 839 557 758 577 496 2348
Fragments arranged by size:
2348 839 758 577 557 496 119 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 741 741
Size: 5747
BsiEI CG_ry'CG
Cuts at: 104 3289 3335 4543 104
Size: 3185 46 1208 1308
Fragments arranged by size:
3185 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 539 1231 2761 3539 3729 4521 539
Size: 692 1530 778 190 792 1765
Fragments arranged by size:
1765 1530 792 778 692 190
BsmI GAATG_Cn'
Cuts at: 1182 1182
Size: 5747
BsmBI CGTCTCn'nnnn_
Cuts at: 710 1954 710
Size: 1244 4503
Bsp1286I G_dGCh'C
Cuts at: 140 539 681 1231 1492 1783 2248 2761 3372
Size: 399 142 550 261 291 465 513 611
Cuts at: 3372 3465 3539 3729 3791 4521 140
Size: 93 74 190 62 730 1366
Fragments arranged by size:
1366 730 611 550 513 465 399 291
261 190 142 93 74 62
BspEI T'CCGG_A
Cuts at: 442 1892 4967 442
Size: 1450 3075 1222
Fragments arranged by size:
3075 1450 1222
BspHI T'CATG_A
Cuts at: 4923 4923
Size: 5747
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2463 2474 2797 3011 3313 3694 2463
Size: 11 323 214 302 381 4516
Fragments arranged by size:
4516 381 323 302 214 11
BsrBI CCG'CTC
Cuts at: 50 2234 2297 4039 4093 5577 5742 50
Size: 2184 63 1742 54 1484 165 55
Fragments arranged by size:
2184 1742 1484 165 63 55 54
BsrDI GCAATG_nn'
Cuts at: 994 1739 3659 994
Size: 745 1920 3082
Fragments arranged by size:
3082 1920 745
BsrFI r'CCGG_y
Cuts at: 2039 2250 3745 3926 4135 2039
Size: 211 1495 181 209 3651
Fragments arranged by size:
3651 1495 211 209 181
BssHII G'CGCG_C
Cuts at: 2611 2611
Size: 5747
BssSI C'ACGA_G
Cuts at: 4018 4376 4018
Size: 358 5389
BstAPI GCAn_nnn'nTGC
Cuts at: 432 984 998 1269 1920 2643 3124 432
Size: 552 14 271 651 723 481 3055
Fragments arranged by size:
3055 723 651 552 481 271 14
BstBI TT'CG_AA
Cuts at: 4108 4108
Size: 5747
BstXI CCAn_nnnn'nTGG
Cuts at: 708 864 708
Size: 156 5591
BstYI r'GATC_y
Cuts at: 1 218 383 512 1721 1757 2078 2387 2673
Size: 217 165 129 1209 36 321 309 286
Cuts at: 2673 2772 3239 3262 3597 3843 4855 4941 4953
Size: 99 467 23 335 246 1012 86 12
Cuts at: 4953 5299 1
Size: 346 449
Fragments arranged by size:
1209 1012 467 449 346 335 321 309
286 246 217 165 129 99 86 36
23 12
BstZ17I GTA'TAC
Cuts at: 845 845
Size: 5747
Bsu36I CC'TnA_GG
Cuts at: 2174 2174
Size: 5747
BtgI C'CryG_G
Cuts at: 107 2663 107
Size: 2556 3191
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 629 3697 3878 4041 629
Size: 3068 181 163 2335
Fragments arranged by size:
3068 2335 181 163
BtsI GCAGTG_nn'
Cuts at: 567 1500 1517 2990 567
Size: 933 17 1473 3324
Fragments arranged by size:
3324 1473 933 17
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 654 689 654
Size: 35 5712
DraI TTT'AAA
Cuts at: 2746 2746
Size: 5747
DraIII CAC_nnn'GTG
Cuts at: 726 726
Size: 5747
DrdI GACnn_nn'nnGTC
Cuts at: 1150 3453 4311 1150
Size: 2303 858 2586
Fragments arranged by size:
2586 2303 858
EaeI y'GGCC_r
Cuts at: 110 413 491 710 717 1904 2414 3332 3506
Size: 303 78 219 7 1187 510 918 174
Cuts at: 3506 3897 3924 5269 110
Size: 391 27 1345 588
Fragments arranged by size:
1345 1187 918 588 510 391 303 219
174 78 27 7
EagI C'GGCC_G
Cuts at: 3332 3332
Size: 5747
EarI CTCTTCn'nnn_
Cuts at: 589 761 825 834 976 1396 3770 3980 589
Size: 172 64 9 142 420 2374 210 2356
Fragments arranged by size:
2374 2356 420 210 172 142 64 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 158 505 1449 1609 2218 2737 3955 4265 4411
Size: 347 944 160 609 519 1218 310 146
Cuts at: 4411 5557 158
Size: 1146 348
Fragments arranged by size:
1218 1146 944 609 519 348 347 310
160 146
EcoICRI GAG'CTC
Cuts at: 1229 2759 1229
Size: 1530 4217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 636 785 1455 1630 1966 2445 2462 2584 3572
Size: 149 670 175 336 479 17 122 988
Cuts at: 3572 4004 4751 5019 636
Size: 432 747 268 1364
Fragments arranged by size:
1364 988 747 670 479 432 336 268
175 149 122 17
EcoNI CCTnn'n_nnAGG
Cuts at: 469 1054 2470 469
Size: 585 1416 3746
Fragments arranged by size:
3746 1416 585
EcoO109I rG'GnC_Cy
Cuts at: 603 677 966 981 1059 1626 2684 2886 603
Size: 74 289 15 78 567 1058 202 3464
Fragments arranged by size:
3464 1058 567 289 202 78 74 15
EcoRI G'AATT_C
Cuts at: 2751 2751
Size: 5747
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 987 3766 987
Size: 2779 2968
FspI TGC'GCA
Cuts at: 2485 3528 2485
Size: 1043 4704
HaeII r_GCGC'y
Cuts at: 553 645 693 1124 1368 1664 2055 3181 3429
Size: 92 48 431 244 296 391 1126 248
Cuts at: 3429 4451 5430 553
Size: 1022 979 870
Fragments arranged by size:
1126 1022 979 870 431 391 296 248
244 92 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 178 210 394 426 2296 2328 3589 3619 3621
Size: 32 184 32 1870 32 1261 30 2
Cuts at: 3621 3651 178
Size: 30 2274
Fragments arranged by size:
2274 1870 1261 184 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2786 2786
Size: 5747
HindIII A'AGCT_T
Cuts at: 1688 1688
Size: 5747
KasI G'GCGC_C
Cuts at: 549 689 1364 3177 3425 549
Size: 140 675 1813 248 2871
Fragments arranged by size:
2871 1813 675 248 140
KpnI G_GTAC'C
Cuts at: 2767 2767
Size: 5747
MluI A'CGCG_T
Cuts at: 5180 5180
Size: 5747
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1736 1991 4418 4602 1736
Size: 255 2427 184 2881
Fragments arranged by size:
2881 2427 255 184
MscI TGG'CCA
Cuts at: 112 712 719 1906 3508 112
Size: 600 7 1187 1602 2351
Fragments arranged by size:
2351 1602 1187 600 7
MslI CAynn'nnrTG
Cuts at: 1190 1705 2600 3736 3863 1190
Size: 515 895 1136 127 3074
Fragments arranged by size:
3074 1136 895 515 127
MspA1I CmG'CkG
Cuts at: 321 429 1434 2345 2440 2643 2858 3172 3532
Size: 108 1005 911 95 203 215 314 360
Cuts at: 3532 4545 4790 321
Size: 1013 245 1278
Fragments arranged by size:
1278 1013 1005 911 360 314 245 215
203 108 95
NaeI GCC'GGC
Cuts at: 2041 2252 3928 2041
Size: 211 1676 3860
Fragments arranged by size:
3860 1676 211
NarI GG'CG_CC
Cuts at: 550 690 1365 3178 3426 550
Size: 140 675 1813 248 2871
Fragments arranged by size:
2871 1813 675 248 140
NcoI C'CATG_G
Cuts at: 107 107
Size: 5747
NgoMIV G'CCGG_C
Cuts at: 2039 2250 3926 2039
Size: 211 1676 3860
Fragments arranged by size:
3860 1676 211
NspI r_CATG'y
Cuts at: 1956 2010 2310 2712 2800 3300 4207 1956
Size: 54 300 402 88 500 907 3496
Fragments arranged by size:
3496 907 500 402 300 88 54
PasI CC'CwG_GG
Cuts at: 1233 1233
Size: 5747
PciI A'CATG_T
Cuts at: 2006 2306 4203 2006
Size: 300 1897 3550
Fragments arranged by size:
3550 1897 300
PflMI CCAn_nnn'nTGG
Cuts at: 2082 2423 2082
Size: 341 5406
PfoI T'CCnGG_A
Cuts at: 452 452
Size: 5747
PmeI GTTT'AAAC
Cuts at: 2746 2746
Size: 5747
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1939 1971 2209 2241 4886 4918 1939
Size: 32 238 32 2645 32 2768
Fragments arranged by size:
2768 2645 238 32 32 32
PspOMI G'GGCC_C
Cuts at: 677 677
Size: 5747
PstI C_TGCA'G
Cuts at: 194 428 1916 2474 2794 3479 194
Size: 234 1488 558 320 685 2462
Fragments arranged by size:
2462 1488 685 558 320 234
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5747
PvuII CAG'CTG
Cuts at: 321 429 2440 3172 3532 321
Size: 108 2011 732 360 2536
Fragments arranged by size:
2536 2011 732 360 108
RsrII CG'GwC_CG
Cuts at: 3942 3942
Size: 5747
SacI G_AGCT'C
Cuts at: 1231 2761 1231
Size: 1530 4217
SalI G'TCGA_C
Cuts at: 2784 2784
Size: 5747
SapI GCTCTTCn'nnn_
Cuts at: 589 761 1396 3770 3980 589
Size: 172 635 2374 210 2356
Fragments arranged by size:
2374 2356 635 210 172
SbfI CC_TGCA'GG
Cuts at: 2794 2794
Size: 5747
ScaI AGT'ACT
Cuts at: 5264 5264
Size: 5747
SexAI A'CCwGG_T
Cuts at: 464 464
Size: 5747
SfcI C'TryA_G
Cuts at: 33 190 424 669 1912 2470 2493 2790 3475
Size: 157 234 245 1243 558 23 297 685
Cuts at: 3475 4468 4659 33
Size: 993 191 1121
Fragments arranged by size:
1243 1121 993 685 558 297 245 234
191 157 23
SfoI GGC'GCC
Cuts at: 551 691 1366 3179 3427 551
Size: 140 675 1813 248 2871
Fragments arranged by size:
2871 1813 675 248 140
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5747
SmaI CCC'GGG
Cuts at: 153 230 1600 1932 2769 153
Size: 77 1370 332 837 3131
Fragments arranged by size:
3131 1370 837 332 77
SmlI C'TyrA_G
Cuts at: 607 961 2902 3817 4309 4571 607
Size: 354 1941 915 492 262 1783
Fragments arranged by size:
1941 1783 915 492 354 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5747
SphI G_CATG'C
Cuts at: 2800 3300 2800
Size: 500 5247
StyI C'CwwG_G
Cuts at: 107 884 2881 3235 107
Size: 777 1997 354 2619
Fragments arranged by size:
2619 1997 777 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1305 1671 2089 3330 3463 3922 4131 1305
Size: 366 418 1241 133 459 209 2921
Fragments arranged by size:
2921 1241 459 418 366 209 133
TatI w'GTAC_w
Cuts at: 5262 5262
Size: 5747
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1363 1395 1551 1583 5264 5296 1363
Size: 32 156 32 3681 32 1814
Fragments arranged by size:
3681 1814 156 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3544 3544
Size: 5747
XbaI T'CTAG_A
Cuts at: 2778 2778
Size: 5747
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1536 2437 3242 1536
Size: 901 805 4041
Fragments arranged by size:
4041 901 805
XmaI C'CCGG_G
Cuts at: 151 228 1598 1930 2767 151
Size: 77 1370 332 837 3131
Fragments arranged by size:
3131 1370 837 332 77
XmnI GAAnn'nnTTC
Cuts at: 2224 2224
Size: 5747
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AscI AvrII BfrBI BmtI BsiWI BsrGI
BstEII ClaI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI