(Circular) (Six-base) MAPSORT of: pF1KE3342.seq Check: 7375 from: 1 to: 6074 pF1KE3342 6074 bp With 182 enzymes: SgfI * March 2, 2011 13:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1966 1966 Size: 6074 AatII G_ACGT'C Cuts at: 1570 1570 Size: 6074 AccI GT'mk_AC Cuts at: 1874 3112 1874 Size: 1238 4836 Acc65I G'GTAC_C Cuts at: 1404 2294 3090 1404 Size: 890 796 4388 Fragments arranged by size: 4388 890 796 AclI AA'CG_TT Cuts at: 5914 5914 Size: 6074 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 217 1582 1618 2074 3899 4331 5078 5346 217 Size: 1365 36 456 1825 432 747 268 945 Fragments arranged by size: 1825 1365 945 747 456 432 268 36 AflIII A'CryG_T Cuts at: 362 3426 4530 5507 362 Size: 3064 1104 977 929 Fragments arranged by size: 3064 1104 977 929 AgeI A'CCGG_T Cuts at: 4462 4462 Size: 6074 AhdI GACnn_n'nnGTC Cuts at: 599 599 Size: 6074 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 682 1806 682 Size: 1124 4950 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1102 1134 1327 1359 1102 Size: 32 193 32 5817 Fragments arranged by size: 5817 193 32 32 AlwNI CAG_nnn'CTG Cuts at: 2899 3379 4946 2899 Size: 480 1567 4027 Fragments arranged by size: 4027 1567 480 ApaI G_GGCC'C Cuts at: 1364 1658 1364 Size: 294 5780 ApaLI G'TGCA_C Cuts at: 1058 4844 1058 Size: 3786 2288 ApoI r'AATT_y Cuts at: 1921 3078 5427 5683 1921 Size: 1157 2349 256 2312 Fragments arranged by size: 2349 2312 1157 256 AseI AT'TA_AT Cuts at: 20 20 Size: 6074 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6074 AvaI C'yCGr_G Cuts at: 306 391 410 743 1052 1256 1478 2283 2771 Size: 85 19 333 309 204 222 805 488 Cuts at: 2771 3036 3094 306 Size: 265 58 3286 Fragments arranged by size: 3286 805 488 333 309 265 222 204 85 58 19 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 1856 1889 1856 Size: 33 6041 BamHI G'GATC_C Cuts at: 3099 3099 Size: 6074 BanI G'GyrC_C Cuts at: 352 652 928 1404 1482 1531 2203 2294 3090 Size: 300 276 476 78 49 672 91 796 Cuts at: 3090 3504 3752 3787 352 Size: 414 248 35 2639 Fragments arranged by size: 2639 796 672 476 414 300 276 248 91 78 49 35 BanII G_rGCy'C Cuts at: 116 1274 1364 1658 2437 3088 4118 116 Size: 1158 90 294 779 651 1030 2072 Fragments arranged by size: 2072 1158 1030 779 651 294 90 BbeI G_GCGC'C Cuts at: 356 1535 2207 3508 3756 356 Size: 1179 672 1301 248 2674 Fragments arranged by size: 2674 1301 1179 672 248 BbsI GAAGACnn'nnnn_ Cuts at: 438 1570 1904 438 Size: 1132 334 4608 Fragments arranged by size: 4608 1132 334 BbvCI CC'TCA_GC Cuts at: 893 2939 893 Size: 2046 4028 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1725 1759 1811 1845 1725 Size: 34 52 34 5954 Fragments arranged by size: 5954 52 34 34 BciVI GTATCCnnnnn_n' Cuts at: 1353 3972 4733 6074 1353 Size: 2619 761 1341 1353 Fragments arranged by size: 2619 1353 1341 761 BclI T'GATC_A Cuts at: 2594 3594 2594 Size: 1000 5074 BglI GCCn_nnn'nGGC Cuts at: 661 1112 2147 2476 661 Size: 451 1035 329 4259 Fragments arranged by size: 4259 1035 451 329 BglII A'GATC_T Cuts at: 2930 3589 5626 2930 Size: 659 2037 3378 Fragments arranged by size: 3378 2037 659 BlpI GC'TnA_GC Cuts at: 613 3186 613 Size: 2573 3501 Bme1580I G_kGCm'C Cuts at: 306 761 924 931 1041 1062 1143 1364 1485 Size: 455 163 7 110 21 81 221 121 Cuts at: 1485 1658 1738 3699 3792 4848 306 Size: 173 80 1961 93 1056 1532 Fragments arranged by size: 1961 1532 1056 455 221 173 163 121 110 93 81 80 21 7 BmgBI CAC'GTC Cuts at: 294 2592 294 Size: 2298 3776 BmrI ACTGGGnnnn_n' Cuts at: 1481 2580 3286 3367 3702 5846 1481 Size: 1099 706 81 335 2144 1709 Fragments arranged by size: 2144 1709 1099 706 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1186 2427 4441 1186 Size: 1241 2014 2819 Fragments arranged by size: 2819 2014 1241 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1168 2164 2422 2538 1168 Size: 996 258 116 4704 Fragments arranged by size: 4704 996 258 116 Bpu10I CC'TnA_GC Cuts at: 893 1350 2939 893 Size: 457 1589 4028 Fragments arranged by size: 4028 1589 457 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1476 1993 3250 4129 4621 4919 1476 Size: 517 1257 879 492 298 2631 Fragments arranged by size: 2631 1257 879 517 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 154 427 1226 2318 5778 154 Size: 273 799 1092 3460 450 Fragments arranged by size: 3460 1092 799 450 273 BsaAI yAC'GTr Cuts at: 363 3427 363 Size: 3064 3010 BsaBI GATnn'nnATC Cuts at: 6 2455 3588 5625 6 Size: 2449 1133 2037 455 Fragments arranged by size: 2449 2037 1133 455 BsaHI Gr'CG_yC Cuts at: 353 1532 1567 2204 3505 3753 4455 5951 353 Size: 1179 35 637 1301 248 702 1496 476 Fragments arranged by size: 1496 1301 1179 702 637 476 248 35 BsaWI w'CCGG_w Cuts at: 2489 2687 2741 3263 3784 4462 4736 4883 5294 Size: 198 54 522 521 678 274 147 411 Cuts at: 5294 2489 Size: 3269 Fragments arranged by size: 3269 678 522 521 411 274 198 147 54 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1059 1089 1102 1132 2419 2449 2533 2551 2563 Size: 30 13 30 1287 30 84 18 12 Cuts at: 2563 2581 2783 2813 1059 Size: 18 202 30 4320 Fragments arranged by size: 4320 1287 202 84 30 30 30 30 18 18 13 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1107 2419 1107 Size: 1312 4762 BseYI C'CCAG_C Cuts at: 131 415 701 805 811 972 1447 1543 3499 Size: 284 286 104 6 161 475 96 1956 Cuts at: 3499 4257 4834 5330 131 Size: 758 577 496 875 Fragments arranged by size: 1956 875 758 577 496 475 286 284 161 104 96 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 316 661 1050 1108 1642 1994 316 Size: 345 389 58 534 352 4396 Fragments arranged by size: 4396 534 389 352 345 58 BsiEI CG_ry'CG Cuts at: 104 3616 3662 4870 104 Size: 3512 46 1208 1308 Fragments arranged by size: 3512 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 116 876 1062 2126 2437 3088 3866 4056 4848 Size: 760 186 1064 311 651 778 190 792 Cuts at: 4848 116 Size: 1342 Fragments arranged by size: 1342 1064 792 778 760 651 311 190 186 BsmI GAATG_Cn' Cuts at: 2697 2697 Size: 6074 Bsp1286I G_dGCh'C Cuts at: 116 306 761 876 924 931 1041 1062 1143 Size: 190 455 115 48 7 110 21 81 Cuts at: 1143 1274 1364 1485 1658 1738 2126 2437 3088 Size: 131 90 121 173 80 388 311 651 Cuts at: 3088 3699 3792 3866 4056 4118 4848 116 Size: 611 93 74 190 62 730 1342 Fragments arranged by size: 1342 730 651 611 455 388 311 190 190 173 131 121 115 110 93 90 81 80 74 62 48 21 7 BspEI T'CCGG_A Cuts at: 2489 2741 5294 2489 Size: 252 2553 3269 Fragments arranged by size: 3269 2553 252 BspHI T'CATG_A Cuts at: 2174 5250 2174 Size: 3076 2998 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1614 1966 2954 3124 3338 3640 4021 1614 Size: 352 988 170 214 302 381 3667 Fragments arranged by size: 3667 988 381 352 302 214 170 BsrBI CCG'CTC Cuts at: 50 245 612 2073 4366 4420 5904 6069 50 Size: 195 367 1461 2293 54 1484 165 55 Fragments arranged by size: 2293 1484 1461 367 195 165 55 54 BsrDI GCAATG_nn' Cuts at: 1815 2480 3986 1815 Size: 665 1506 3903 Fragments arranged by size: 3903 1506 665 BsrFI r'CCGG_y Cuts at: 766 852 1645 2618 3054 4072 4253 4462 766 Size: 86 793 973 436 1018 181 209 2378 Fragments arranged by size: 2378 1018 973 793 436 209 181 86 BssHII G'CGCG_C Cuts at: 1633 1633 Size: 6074 BssSI C'ACGA_G Cuts at: 2257 2430 4345 4703 2257 Size: 173 1915 358 3628 Fragments arranged by size: 3628 1915 358 173 BstAPI GCAn_nnn'nTGC Cuts at: 270 3451 270 Size: 3181 2893 BstBI TT'CG_AA Cuts at: 4435 4435 Size: 6074 BstEII G'GTnAC_C Cuts at: 2154 2154 Size: 6074 BstXI CCAn_nnnn'nTGG Cuts at: 1617 2868 1617 Size: 1251 4823 BstYI r'GATC_y Cuts at: 1 2930 3099 3566 3589 3924 4170 5182 5268 Size: 2929 169 467 23 335 246 1012 86 Cuts at: 5268 5280 5626 1 Size: 12 346 449 Fragments arranged by size: 2929 1012 467 449 346 335 246 169 86 23 12 Bsu36I CC'TnA_GG Cuts at: 215 1795 215 Size: 1580 4494 BtgI C'CryG_G Cuts at: 107 343 476 533 761 1087 1355 107 Size: 236 133 57 228 326 268 4826 Fragments arranged by size: 4826 326 268 236 228 133 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 476 2262 4024 4205 4368 476 Size: 1786 1762 181 163 2182 Fragments arranged by size: 2182 1786 1762 181 163 BtsI GCAGTG_nn' Cuts at: 235 1325 1421 3317 235 Size: 1090 96 1896 2992 Fragments arranged by size: 2992 1896 1090 96 ClaI AT'CG_AT Cuts at: 1937 1937 Size: 6074 DraI TTT'AAA Cuts at: 3073 3073 Size: 6074 DraIII CAC_nnn'GTG Cuts at: 270 908 1489 270 Size: 638 581 4855 Fragments arranged by size: 4855 638 581 DrdI GACnn_nn'nnGTC Cuts at: 1510 3780 4638 1510 Size: 2270 858 2946 Fragments arranged by size: 2946 2270 858 EaeI y'GGCC_r Cuts at: 143 473 530 764 834 1158 1662 2993 3659 Size: 330 57 234 70 324 504 1331 666 Cuts at: 3659 3833 4224 4251 5596 143 Size: 174 391 27 1345 621 Fragments arranged by size: 1345 1331 666 621 504 391 330 324 234 174 70 57 27 EagI C'GGCC_G Cuts at: 3659 3659 Size: 6074 EarI CTCTTCn'nnn_ Cuts at: 152 688 1955 2418 4097 4307 152 Size: 536 1267 463 1679 210 1919 Fragments arranged by size: 1919 1679 1267 536 463 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2128 2493 4282 4592 4738 5884 2128 Size: 365 1789 310 146 1146 2318 Fragments arranged by size: 2318 1789 1146 365 310 146 EcoICRI GAG'CTC Cuts at: 114 2435 3086 114 Size: 2321 651 3102 Fragments arranged by size: 3102 2321 651 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 217 1168 1582 1618 2074 2164 2422 2538 3899 Size: 951 414 36 456 90 258 116 1361 Cuts at: 3899 4331 5078 5346 217 Size: 432 747 268 945 Fragments arranged by size: 1361 951 945 747 456 432 414 268 258 116 90 36 EcoNI CCTnn'n_nnAGG Cuts at: 253 1621 253 Size: 1368 4706 EcoO109I rG'GnC_Cy Cuts at: 339 956 1167 1260 1360 1540 1655 2013 2786 Size: 617 211 93 100 180 115 358 773 Cuts at: 2786 2943 3213 339 Size: 157 270 3200 Fragments arranged by size: 3200 773 617 358 270 211 180 157 115 100 93 EcoRI G'AATT_C Cuts at: 3078 3078 Size: 6074 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 453 642 3006 4093 453 Size: 189 2364 1087 2434 Fragments arranged by size: 2434 2364 1087 189 FspI TGC'GCA Cuts at: 274 3855 274 Size: 3581 2493 FspAI rTGC'GCAy Cuts at: 274 274 Size: 6074 HaeII r_GCGC'y Cuts at: 356 423 558 1535 2207 2726 3508 3756 4778 Size: 67 135 977 672 519 782 248 1022 Cuts at: 4778 5757 356 Size: 979 673 Fragments arranged by size: 1022 979 977 782 673 672 519 248 135 67 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 198 230 385 417 585 617 726 758 992 Size: 32 155 32 168 32 109 32 234 Cuts at: 992 1024 1835 1867 2533 2565 3916 3946 3948 Size: 32 811 32 666 32 1351 30 2 Cuts at: 3948 3978 198 Size: 30 2294 Fragments arranged by size: 2294 1351 811 666 234 168 155 109 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 2352 3113 2352 Size: 761 5313 KasI G'GCGC_C Cuts at: 352 1531 2203 3504 3752 352 Size: 1179 672 1301 248 2674 Fragments arranged by size: 2674 1301 1179 672 248 KpnI G_GTAC'C Cuts at: 1408 2298 3094 1408 Size: 890 796 4388 Fragments arranged by size: 4388 890 796 MluI A'CGCG_T Cuts at: 5507 5507 Size: 6074 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1445 4745 4929 1445 Size: 3300 184 2590 Fragments arranged by size: 3300 2590 184 MscI TGG'CCA Cuts at: 836 2995 3835 836 Size: 2159 840 3075 Fragments arranged by size: 3075 2159 840 MslI CAynn'nnrTG Cuts at: 517 1429 2866 4063 4190 517 Size: 912 1437 1197 127 2401 Fragments arranged by size: 2401 1437 1197 912 127 MspA1I CmG'CkG Cuts at: 535 705 869 892 1089 1187 1719 2079 2811 Size: 170 164 23 197 98 532 360 732 Cuts at: 2811 2899 3185 3499 3859 4872 5117 535 Size: 88 286 314 360 1013 245 1492 Fragments arranged by size: 1492 1013 732 532 360 360 314 286 245 197 170 164 98 88 23 NaeI GCC'GGC Cuts at: 1647 4255 1647 Size: 2608 3466 NarI GG'CG_CC Cuts at: 353 1532 2204 3505 3753 353 Size: 1179 672 1301 248 2674 Fragments arranged by size: 2674 1301 1179 672 248 NcoI C'CATG_G Cuts at: 107 107 Size: 6074 NgoMIV G'CCGG_C Cuts at: 1645 4253 1645 Size: 2608 3466 NspI r_CATG'y Cuts at: 2169 2268 2292 3127 3627 4534 2169 Size: 99 24 835 500 907 3709 Fragments arranged by size: 3709 907 835 500 99 24 PasI CC'CwG_GG Cuts at: 559 924 1274 2017 2439 559 Size: 365 350 743 422 4194 Fragments arranged by size: 4194 743 422 365 350 PciI A'CATG_T Cuts at: 4530 4530 Size: 6074 PflMI CCAn_nnn'nTGG Cuts at: 2259 2462 2259 Size: 203 5871 PfoI T'CCnGG_A Cuts at: 641 728 2399 2801 641 Size: 87 1671 402 3914 Fragments arranged by size: 3914 1671 402 87 PmeI GTTT'AAAC Cuts at: 3073 3073 Size: 6074 PmlI CAC'GTG Cuts at: 363 363 Size: 6074 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1102 1134 5213 5245 1102 Size: 32 4079 32 1931 Fragments arranged by size: 4079 1931 32 32 PpuMI rG'GwC_Cy Cuts at: 339 956 1167 1260 1540 2943 339 Size: 617 211 93 280 1403 3470 Fragments arranged by size: 3470 1403 617 280 211 93 PspOMI G'GGCC_C Cuts at: 1360 1654 1360 Size: 294 5780 PspXI vC'TCGA_Gb Cuts at: 391 391 Size: 6074 PstI C_TGCA'G Cuts at: 868 1416 3121 3806 868 Size: 548 1705 685 3136 Fragments arranged by size: 3136 1705 685 548 PvuI CG_AT'CG Cuts at: 104 104 Size: 6074 PvuII CAG'CTG Cuts at: 705 869 892 1719 3499 3859 705 Size: 164 23 827 1780 360 2920 Fragments arranged by size: 2920 1780 827 360 164 23 RsrII CG'GwC_CG Cuts at: 4269 4269 Size: 6074 SacI G_AGCT'C Cuts at: 116 2437 3088 116 Size: 2321 651 3102 Fragments arranged by size: 3102 2321 651 SacII CC_GC'GG Cuts at: 536 1090 536 Size: 554 5520 SalI G'TCGA_C Cuts at: 3111 3111 Size: 6074 SanDI GG'GwC_CC Cuts at: 339 1540 339 Size: 1201 4873 SapI GCTCTTCn'nnn_ Cuts at: 4097 4307 4097 Size: 210 5864 SbfI CC_TGCA'GG Cuts at: 3121 3121 Size: 6074 ScaI AGT'ACT Cuts at: 5591 5591 Size: 6074 SexAI A'CCwGG_T Cuts at: 1262 1616 1262 Size: 354 5720 SfcI C'TryA_G Cuts at: 33 864 1412 3117 3802 4795 4986 33 Size: 831 548 1705 685 993 191 1121 Fragments arranged by size: 1705 1121 993 831 685 548 191 SfoI GGC'GCC Cuts at: 354 1533 2205 3506 3754 354 Size: 1179 672 1301 248 2674 Fragments arranged by size: 2674 1301 1179 672 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6074 SmaI CCC'GGG Cuts at: 308 1054 1258 1480 2773 3038 3096 308 Size: 746 204 222 1293 265 58 3286 Fragments arranged by size: 3286 1293 746 265 222 204 58 SmlI C'TyrA_G Cuts at: 391 1455 2008 3229 4144 4636 4898 391 Size: 1064 553 1221 915 492 262 1567 Fragments arranged by size: 1567 1221 1064 915 553 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 6074 SphI G_CATG'C Cuts at: 2169 2268 2292 3127 3627 2169 Size: 99 24 835 500 4616 Fragments arranged by size: 4616 835 500 99 24 SspI AAT'ATT Cuts at: 1828 1828 Size: 6074 StuI AGG'CCT Cuts at: 2101 2101 Size: 6074 StyI C'CwwG_G Cuts at: 107 3208 3562 107 Size: 3101 354 2619 Fragments arranged by size: 3101 2619 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 192 1266 1412 1461 1500 1738 3657 3790 4249 Size: 1074 146 49 39 238 1919 133 459 Cuts at: 4249 4458 192 Size: 209 1808 Fragments arranged by size: 1919 1808 1074 459 238 209 146 133 49 39 TatI w'GTAC_w Cuts at: 5589 5589 Size: 6074 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 214 246 823 855 1059 1091 2783 2815 2998 Size: 32 577 32 204 32 1692 32 183 Cuts at: 2998 3030 5591 5623 214 Size: 32 2561 32 665 Fragments arranged by size: 2561 1692 665 577 204 183 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1437 3871 1437 Size: 2434 3640 XbaI T'CTAG_A Cuts at: 3105 3105 Size: 6074 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3569 3569 Size: 6074 XhoI C'TCGA_G Cuts at: 391 391 Size: 6074 XmaI C'CCGG_G Cuts at: 306 1052 1256 1478 2771 3036 3094 306 Size: 746 204 222 1293 265 58 3286 Fragments arranged by size: 3286 1293 746 265 222 204 58 ZraI GAC'GTC Cuts at: 1568 1568 Size: 6074 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI Enzymes that do not cut: AfeI AflII AleI AscI AvrII BfrBI BmtI BsiWI BsmBI BsrGI BstZ17I CspCI EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PshAI PsiI PsrI SfiI SgrAI SnaBI SrfI SwaI XmnI