Restriction map for
pF1KE3359
(Circular) (Six-base) MAPSORT of: pF1KE3359.seq Check: 4600 from: 1 to: 6296
pF1KE3359 6296 bp
With 182 enzymes: SgfI *
September 19, 2014 17:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1685 2784 1685
Size: 1099 5197
AccI GT'mk_AC
Cuts at: 2273 3334 2273
Size: 1061 5235
Acc65I G'GTAC_C
Cuts at: 1445 3312 1445
Size: 1867 4429
AclI AA'CG_TT
Cuts at: 6136 6136
Size: 6296
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 843 1105 1350 1879 2142 2278 2710 2727 3088
Size: 262 245 529 263 136 432 17 361
Cuts at: 3088 3163 4121 4553 5300 5568 843
Size: 75 958 432 747 268 1571
Fragments arranged by size:
1571 958 747 529 432 432 361 268
263 262 245 136 75 17
AfeI AGC'GCT
Cuts at: 653 2768 653
Size: 2115 4181
AflIII A'CryG_T
Cuts at: 1809 3648 4752 5729 1809
Size: 1839 1104 977 2376
Fragments arranged by size:
2376 1839 1104 977
AgeI A'CCGG_T
Cuts at: 4684 4684
Size: 6296
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2806 2806
Size: 6296
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 700 732 1993 2025 700
Size: 32 1261 32 4971
Fragments arranged by size:
4971 1261 32 32
AlwNI CAG_nnn'CTG
Cuts at: 980 2664 2680 3043 3601 5168 980
Size: 1684 16 363 558 1567 2108
Fragments arranged by size:
2108 1684 1567 558 363 16
ApaI G_GGCC'C
Cuts at: 119 454 587 964 119
Size: 335 133 377 5451
Fragments arranged by size:
5451 377 335 133
ApaLI G'TGCA_C
Cuts at: 5066 5066
Size: 6296
ApoI r'AATT_y
Cuts at: 1078 2515 3300 5649 5905 1078
Size: 1437 785 2349 256 1469
Fragments arranged by size:
2349 1469 1437 785 256
AscI GG'CGCG_CC
Cuts at: 310 310
Size: 6296
AseI AT'TA_AT
Cuts at: 20 20
Size: 6296
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6296
AvaI C'yCGr_G
Cuts at: 1066 1351 2375 3284 3316 1066
Size: 285 1024 909 32 4046
Fragments arranged by size:
4046 1024 909 285 32
BamHI G'GATC_C
Cuts at: 1347 3321 1347
Size: 1974 4322
BanI G'GyrC_C
Cuts at: 122 526 1360 1445 1673 3312 3726 3974 4009
Size: 404 834 85 228 1639 414 248 35
Cuts at: 4009 122
Size: 2409
Fragments arranged by size:
2409 1639 834 414 404 248 228 85
35
BanII G_rGCy'C
Cuts at: 119 454 587 964 1178 1658 2225 2257 2350
Size: 335 133 377 214 480 567 32 93
Cuts at: 2350 2906 3310 4340 119
Size: 556 404 1030 2075
Fragments arranged by size:
2075 1030 567 556 480 404 377 335
214 133 93 32
BbeI G_GCGC'C
Cuts at: 126 530 3730 3978 126
Size: 404 3200 248 2444
Fragments arranged by size:
3200 2444 404 248
BbsI GAAGACnn'nnnn_
Cuts at: 1493 2313 3149 1493
Size: 820 836 4640
Fragments arranged by size:
4640 836 820
BbvCI CC'TCA_GC
Cuts at: 781 2462 2642 781
Size: 1681 180 4435
Fragments arranged by size:
4435 1681 180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1342 1376 90
Size: 34 1218 34 5010
Fragments arranged by size:
5010 1218 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1282 3090 4194 4955 6296 1282
Size: 1808 1104 761 1341 1282
Fragments arranged by size:
1808 1341 1282 1104 761
BclI T'GATC_A
Cuts at: 3816 3816
Size: 6296
BglI GCCn_nnn'nGGC
Cuts at: 131 203 1018 1027 2950 131
Size: 72 815 9 1923 3477
Fragments arranged by size:
3477 1923 815 72 9
BglII A'GATC_T
Cuts at: 3811 5848 3811
Size: 2037 4259
BlpI GC'TnA_GC
Cuts at: 2681 3408 2681
Size: 727 5569
Bme1580I G_kGCm'C
Cuts at: 119 454 587 964 1014 3921 4014 5070 119
Size: 335 133 377 50 2907 93 1056 1345
Fragments arranged by size:
2907 1345 1056 377 335 133 93 50
BmrI ACTGGGnnnn_n'
Cuts at: 472 1652 3508 3589 3924 6068 472
Size: 1180 1856 81 335 2144 700
Fragments arranged by size:
2144 1856 1180 700 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4663 4663
Size: 6296
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 1543 1624 1999 2170 2446 2608 2644 3074
Size: 762 81 375 171 276 162 36 430
Cuts at: 3074 3199 3223 781
Size: 125 24 3854
Fragments arranged by size:
3854 762 430 375 276 171 162 125
81 36 24
Bpu10I CC'TnA_GC
Cuts at: 781 1705 2219 2344 2462 2642 781
Size: 924 514 125 118 180 4435
Fragments arranged by size:
4435 924 514 180 125 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 1687 2074 2113 3099 3472 4351 4843 5141
Size: 1284 387 39 986 373 879 492 298
Cuts at: 5141 403
Size: 1558
Fragments arranged by size:
1558 1284 986 879 492 387 373 298
39
BsaI GGTCTCn'nnnn_
Cuts at: 3157 6000 3157
Size: 2843 3453
BsaAI yAC'GTr
Cuts at: 2328 3649 2328
Size: 1321 4975
BsaBI GATnn'nnATC
Cuts at: 6 1346 3810 5847 6
Size: 1340 2464 2037 455
Fragments arranged by size:
2464 2037 1340 455
BsaHI Gr'CG_yC
Cuts at: 123 377 527 3727 3975 4677 6173 123
Size: 254 150 3200 248 702 1496 246
Fragments arranged by size:
3200 1496 702 254 248 246 150
BsaWI w'CCGG_w
Cuts at: 2012 3485 4006 4684 4958 5105 5516 2012
Size: 1473 521 678 274 147 411 2792
Fragments arranged by size:
2792 1473 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 700 730 700
Size: 30 6266
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1442 2325 2443 2617 2641 3196 1442
Size: 883 118 174 24 555 4542
Fragments arranged by size:
4542 883 555 174 118 24
BseYI C'CCAG_C
Cuts at: 351 372 537 586 600 633 638 1155 1491
Size: 21 165 49 14 33 5 517 336
Cuts at: 1491 1640 2456 3721 4479 5056 5552 351
Size: 149 816 1265 758 577 496 1095
Fragments arranged by size:
1265 1095 816 758 577 517 496 336
165 149 49 33 21 14 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 1706 1896 1897 2728 607
Size: 1099 190 1 831 4175
Fragments arranged by size:
4175 1099 831 190 1
BsiEI CG_ry'CG
Cuts at: 104 676 735 1317 3838 3884 5092 104
Size: 572 59 582 2521 46 1208 1308
Fragments arranged by size:
2521 1308 1208 582 572 59 46
BsiHKAI G_wGCw'C
Cuts at: 431 692 2129 2225 2785 3310 4088 4278 5070
Size: 261 1437 96 560 525 778 190 792
Cuts at: 5070 431
Size: 1657
Fragments arranged by size:
1657 1437 792 778 560 525 261 190
96
BsmI GAATG_Cn'
Cuts at: 1562 1562
Size: 6296
BsmBI CGTCTCn'nnnn_
Cuts at: 2695 2695
Size: 6296
Bsp1286I G_dGCh'C
Cuts at: 119 431 454 587 692 964 1014 1178 1658
Size: 312 23 133 105 272 50 164 480
Cuts at: 1658 2129 2225 2257 2350 2785 2906 3310 3921
Size: 471 96 32 93 435 121 404 611
Cuts at: 3921 4014 4088 4278 4340 5070 119
Size: 93 74 190 62 730 1345
Fragments arranged by size:
1345 730 611 480 471 435 404 312
272 190 164 133 121 105 96 93
93 74 62 50 32 23
BspEI T'CCGG_A
Cuts at: 5516 5516
Size: 6296
BspHI T'CATG_A
Cuts at: 5472 5472
Size: 6296
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1685 2561 2784 3346 3560 3862 4243 1685
Size: 876 223 562 214 302 381 3738
Fragments arranged by size:
3738 876 562 381 302 223 214
BsrBI CCG'CTC
Cuts at: 50 1709 2367 2607 4588 4642 6126 6291 50
Size: 1659 658 240 1981 54 1484 165 55
Fragments arranged by size:
1981 1659 1484 658 240 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4208 4208
Size: 6296
BsrFI r'CCGG_y
Cuts at: 523 671 1694 2996 4294 4475 4684 523
Size: 148 1023 1302 1298 181 209 2135
Fragments arranged by size:
2135 1302 1298 1023 209 181 148
BsrGI T'GTAC_A
Cuts at: 2819 2858 2819
Size: 39 6257
BssHII G'CGCG_C
Cuts at: 265 310 265
Size: 45 6251
BssSI C'ACGA_G
Cuts at: 703 1795 4567 4925 703
Size: 1092 2772 358 2074
Fragments arranged by size:
2772 2074 1092 358
BstAPI GCAn_nnn'nTGC
Cuts at: 945 3673 945
Size: 2728 3568
BstBI TT'CG_AA
Cuts at: 4657 4657
Size: 6296
BstEII G'GTnAC_C
Cuts at: 2799 2799
Size: 6296
BstXI CCAn_nnnn'nTGG
Cuts at: 1902 2106 2796 1902
Size: 204 690 5402
Fragments arranged by size:
5402 690 204
BstYI r'GATC_y
Cuts at: 1 1347 2495 2660 3146 3321 3788 3811 4146
Size: 1346 1148 165 486 175 467 23 335
Cuts at: 4146 4392 5404 5490 5502 5848 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1346 1148 1012 486 467 449 346 335
246 175 165 86 23 12
BstZ17I GTA'TAC
Cuts at: 2274 2274
Size: 6296
Bsu36I CC'TnA_GG
Cuts at: 2308 2308
Size: 6296
BtgI C'CryG_G
Cuts at: 221 281 407 1127 1956 2079 221
Size: 60 126 720 829 123 4438
Fragments arranged by size:
4438 829 720 126 123 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2894 4246 4427 4590 2894
Size: 1352 181 163 4600
Fragments arranged by size:
4600 1352 181 163
BtsI GCAGTG_nn'
Cuts at: 690 1202 1314 2959 3539 690
Size: 512 112 1645 580 3447
Fragments arranged by size:
3447 1645 580 512 112
DraI TTT'AAA
Cuts at: 3295 3295
Size: 6296
DraIII CAC_nnn'GTG
Cuts at: 2676 2676
Size: 6296
DrdI GACnn_nn'nnGTC
Cuts at: 4002 4860 4002
Size: 858 5438
EaeI y'GGCC_r
Cuts at: 236 404 673 3881 4055 4446 4473 5818 236
Size: 168 269 3208 174 391 27 1345 714
Fragments arranged by size:
3208 1345 714 391 269 174 168 27
EagI C'GGCC_G
Cuts at: 673 3881 673
Size: 3208 3088
EarI CTCTTCn'nnn_
Cuts at: 680 4319 4529 680
Size: 3639 210 2447
Fragments arranged by size:
3639 2447 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2488 2791 2980 4504 4814 4960 6106 2488
Size: 303 189 1524 310 146 1146 2678
Fragments arranged by size:
2678 1524 1146 310 303 189 146
EcoICRI GAG'CTC
Cuts at: 2223 3308 2223
Size: 1085 5211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 843 1105 1350 1543 1624 1879 1999 2142
Size: 62 262 245 193 81 255 120 143
Cuts at: 2142 2170 2278 2446 2608 2644 2710 2727 3074
Size: 28 108 168 162 36 66 17 347
Cuts at: 3074 3088 3163 3199 3223 4121 4553 5300 5568
Size: 14 75 36 24 898 432 747 268
Cuts at: 5568 781
Size: 1509
Fragments arranged by size:
1509 898 747 432 347 268 262 255
245 193 168 162 143 120 108 81
75 66 62 36 36 28 24 17
14
EcoNI CCTnn'n_nnAGG
Cuts at: 298 706 298
Size: 408 5888
EcoO109I rG'GnC_Cy
Cuts at: 115 116 274 451 507 583 960 961 977
Size: 1 158 177 56 76 377 1 16
Cuts at: 977 2163 2466 2646 3040 3435 115
Size: 1186 303 180 394 395 2976
Fragments arranged by size:
2976 1186 395 394 377 303 180 177
158 76 56 16 1 1
EcoRI G'AATT_C
Cuts at: 1078 3300 1078
Size: 2222 4074
EcoRV GAT'ATC
Cuts at: 930 930
Size: 6296
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1528 3168 4315 1528
Size: 1640 1147 3509
Fragments arranged by size:
3509 1640 1147
FspI TGC'GCA
Cuts at: 2512 3022 4077 2512
Size: 510 1055 4731
Fragments arranged by size:
4731 1055 510
HaeII r_GCGC'y
Cuts at: 126 400 489 530 538 622 648 655 2121
Size: 274 89 41 8 84 26 7 1466
Cuts at: 2121 2770 3730 3978 5000 5979 126
Size: 649 960 248 1022 979 443
Fragments arranged by size:
1466 1022 979 960 649 443 274 248
89 84 41 26 8 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1191 1223 2652 2684 4138 4168 4170 4200 1191
Size: 32 1429 32 1454 30 2 30 3287
Fragments arranged by size:
3287 1454 1429 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3105 3335 3105
Size: 230 6066
HindIII A'AGCT_T
Cuts at: 1269 1269
Size: 6296
KasI G'GCGC_C
Cuts at: 122 526 3726 3974 122
Size: 404 3200 248 2444
Fragments arranged by size:
3200 2444 404 248
KpnI G_GTAC'C
Cuts at: 1449 3316 1449
Size: 1867 4429
MfeI C'AATT_G
Cuts at: 2873 2873
Size: 6296
MluI A'CGCG_T
Cuts at: 5729 5729
Size: 6296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 410 2123 4967 5151 410
Size: 1713 2844 184 1555
Fragments arranged by size:
2844 1713 1555 184
MscI TGG'CCA
Cuts at: 238 4057 238
Size: 3819 2477
MslI CAynn'nnrTG
Cuts at: 2788 2794 2893 4285 4412 2788
Size: 6 99 1392 127 4672
Fragments arranged by size:
4672 1392 127 99 6
MspA1I CmG'CkG
Cuts at: 130 174 234 409 439 513 1254 1602 2451
Size: 44 60 175 30 74 741 348 849
Cuts at: 2451 2688 3012 3407 3721 4081 5094 5339 130
Size: 237 324 395 314 360 1013 245 1087
Fragments arranged by size:
1087 1013 849 741 395 360 348 324
314 245 237 175 74 60 44 30
NaeI GCC'GGC
Cuts at: 525 1696 4477 525
Size: 1171 2781 2344
Fragments arranged by size:
2781 2344 1171
NarI GG'CG_CC
Cuts at: 123 527 3727 3975 123
Size: 404 3200 248 2444
Fragments arranged by size:
3200 2444 404 248
NcoI C'CATG_G
Cuts at: 1127 2079 1127
Size: 952 5344
NdeI CA'TA_TG
Cuts at: 1865 1865
Size: 6296
NgoMIV G'CCGG_C
Cuts at: 523 1694 4475 523
Size: 1171 2781 2344
Fragments arranged by size:
2781 2344 1171
NspI r_CATG'y
Cuts at: 1499 2049 3349 3849 4756 1499
Size: 550 1300 500 907 3039
Fragments arranged by size:
3039 1300 907 550 500
PasI CC'CwG_GG
Cuts at: 980 1440 980
Size: 460 5836
PciI A'CATG_T
Cuts at: 4752 4752
Size: 6296
PflMI CCAn_nnn'nTGG
Cuts at: 1404 1404
Size: 6296
PfoI T'CCnGG_A
Cuts at: 2543 3176 2543
Size: 633 5663
PmeI GTTT'AAAC
Cuts at: 3295 3295
Size: 6296
PmlI CAC'GTG
Cuts at: 2328 2328
Size: 6296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 700 732 1738 1770 5435 5467 700
Size: 32 1006 32 3665 32 1529
Fragments arranged by size:
3665 1529 1006 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 274 507 2163 2466 3040 274
Size: 233 1656 303 574 3530
Fragments arranged by size:
3530 1656 574 303 233
PsiI TTA'TAA
Cuts at: 1784 1784
Size: 6296
PspOMI G'GGCC_C
Cuts at: 115 450 583 960 115
Size: 335 133 377 5451
Fragments arranged by size:
5451 377 335 133
PstI C_TGCA'G
Cuts at: 844 1970 2417 3343 4028 844
Size: 1126 447 926 685 3112
Fragments arranged by size:
3112 1126 926 685 447
PvuI CG_AT'CG
Cuts at: 104 1317 104
Size: 1213 5083
PvuII CAG'CTG
Cuts at: 1602 2688 3012 3721 4081 1602
Size: 1086 324 709 360 3817
Fragments arranged by size:
3817 1086 709 360 324
RsrII CG'GwC_CG
Cuts at: 339 4491 339
Size: 4152 2144
SacI G_AGCT'C
Cuts at: 2225 3310 2225
Size: 1085 5211
SacII CC_GC'GG
Cuts at: 410 410
Size: 6296
SalI G'TCGA_C
Cuts at: 3333 3333
Size: 6296
SanDI GG'GwC_CC
Cuts at: 274 507 274
Size: 233 6063
SapI GCTCTTCn'nnn_
Cuts at: 680 4319 4529 680
Size: 3639 210 2447
Fragments arranged by size:
3639 2447 210
SbfI CC_TGCA'GG
Cuts at: 1970 3343 1970
Size: 1373 4923
ScaI AGT'ACT
Cuts at: 1072 1948 5813 1072
Size: 876 3865 1555
Fragments arranged by size:
3865 1555 876
SexAI A'CCwGG_T
Cuts at: 1894 1894
Size: 6296
SfcI C'TryA_G
Cuts at: 33 840 1966 2413 3339 4024 5017 5208 33
Size: 807 1126 447 926 685 993 191 1121
Fragments arranged by size:
1126 1121 993 926 807 685 447 191
SfoI GGC'GCC
Cuts at: 124 528 3728 3976 124
Size: 404 3200 248 2444
Fragments arranged by size:
3200 2444 404 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6296
SgrAI Cr'CCGG_yG
Cuts at: 523 523
Size: 6296
SmaI CCC'GGG
Cuts at: 3318 3318
Size: 6296
SmlI C'TyrA_G
Cuts at: 418 1666 2089 2128 3114 3451 4366 4858 5120
Size: 1248 423 39 986 337 915 492 262
Cuts at: 5120 418
Size: 1594
Fragments arranged by size:
1594 1248 986 915 492 423 337 262
39
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6296
SphI G_CATG'C
Cuts at: 1499 2049 3349 3849 1499
Size: 550 1300 500 3946
Fragments arranged by size:
3946 1300 550 500
SspI AAT'ATT
Cuts at: 744 744
Size: 6296
StuI AGG'CCT
Cuts at: 1021 1021
Size: 6296
StyI C'CwwG_G
Cuts at: 1127 2079 3167 3261 3430 3784 1127
Size: 952 1088 94 169 354 3639
Fragments arranged by size:
3639 1088 952 354 169 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2802 3879 4012 4471 4680 2802
Size: 1077 133 459 209 4418
Fragments arranged by size:
4418 1077 459 209 133
TatI w'GTAC_w
Cuts at: 1070 1374 1946 2819 2858 5811 1070
Size: 304 572 873 39 2953 1555
Fragments arranged by size:
2953 1555 873 572 304 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 266 298 406 438 1829 1861 5813 5845 266
Size: 32 108 32 1391 32 3952 32 717
Fragments arranged by size:
3952 1391 717 108 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2035 4093 2035
Size: 2058 4238
XbaI T'CTAG_A
Cuts at: 3327 3327
Size: 6296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3174 3791 3174
Size: 617 5679
XmaI C'CCGG_G
Cuts at: 3316 3316
Size: 6296
XmnI GAAnn'nnTTC
Cuts at: 2235 2235
Size: 6296
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AvrII BaeI BfrBI BmgBI
BmtI BsiWI ClaI CspCI FseI FspAI HpaI NheI
NotI NruI NsiI PacI PshAI PspXI PsrI SfiI
SnaBI SrfI SwaI XhoI ZraI