Restriction map for
pF1KE3369
(Circular) (Six-base) MAPSORT of: pF1KE3369.seq Check: 1686 from: 1 to: 6380
pF1KE3369 6380 bp
With 182 enzymes: SgfI *
November 11, 2016 16:52 ..
AccI GT'mk_AC
Cuts at: 2882 3418 2882
Size: 536 5844
Acc65I G'GTAC_C
Cuts at: 609 2843 3396 609
Size: 2234 553 3593
Fragments arranged by size:
3593 2234 553
AclI AA'CG_TT
Cuts at: 6220 6220
Size: 6380
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 1053 1439 1669 2062 3037 3127 3263 3332
Size: 739 386 230 393 975 90 136 69
Cuts at: 3332 4205 4637 5384 5652 314
Size: 873 432 747 268 1042
Fragments arranged by size:
1042 975 873 747 739 432 393 386
268 230 136 90 69
AfeI AGC'GCT
Cuts at: 3147 3147
Size: 6380
AflIII A'CryG_T
Cuts at: 3732 4836 5813 3732
Size: 1104 977 4299
Fragments arranged by size:
4299 1104 977
AgeI A'CCGG_T
Cuts at: 4768 4768
Size: 6380
AleI CACnn'nnGTG
Cuts at: 596 1204 2945 596
Size: 608 1741 4031
Fragments arranged by size:
4031 1741 608
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 901 901
Size: 6380
AlwNI CAG_nnn'CTG
Cuts at: 3141 3685 5252 3141
Size: 544 1567 4269
Fragments arranged by size:
4269 1567 544
ApaI G_GGCC'C
Cuts at: 665 665
Size: 6380
ApaLI G'TGCA_C
Cuts at: 1410 5150 1410
Size: 3740 2640
ApoI r'AATT_y
Cuts at: 231 1775 2999 3384 5733 5989 231
Size: 1544 1224 385 2349 256 622
Fragments arranged by size:
2349 1544 1224 622 385 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6380
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6380
AvaI C'yCGr_G
Cuts at: 571 940 1541 2516 3400 571
Size: 369 601 975 884 3551
Fragments arranged by size:
3551 975 884 601 369
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1536 1569 1536
Size: 33 6347
BamHI G'GATC_C
Cuts at: 3405 3405
Size: 6380
BanI G'GyrC_C
Cuts at: 609 2785 2843 3396 3810 4058 4093 609
Size: 2176 58 553 414 248 35 2896
Fragments arranged by size:
2896 2176 553 414 248 58 35
BanII G_rGCy'C
Cuts at: 307 665 3394 4424 307
Size: 358 2729 1030 2263
Fragments arranged by size:
2729 2263 1030 358
BbeI G_GCGC'C
Cuts at: 3814 4062 3814
Size: 248 6132
BbsI GAAGACnn'nnnn_
Cuts at: 139 1274 3104 3317 3368 139
Size: 1135 1830 213 51 3151
Fragments arranged by size:
3151 1830 1135 213 51
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 961 995 1827 1861 961
Size: 34 832 34 5480
Fragments arranged by size:
5480 832 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4278 5039 6380 4278
Size: 761 1341 4278
Fragments arranged by size:
4278 1341 761
BclI T'GATC_A
Cuts at: 1374 1721 3900 1374
Size: 347 2179 3854
Fragments arranged by size:
3854 2179 347
BfrBI ATG'CAT
Cuts at: 2640 3067 2640
Size: 427 5953
BglI GCCn_nnn'nGGC
Cuts at: 382 988 382
Size: 606 5774
BglII A'GATC_T
Cuts at: 2606 2768 3895 5932 2606
Size: 162 1127 2037 3054
Fragments arranged by size:
3054 2037 1127 162
BlpI GC'TnA_GC
Cuts at: 160 3492 160
Size: 3332 3048
Bme1580I G_kGCm'C
Cuts at: 665 1414 4005 4098 5154 665
Size: 749 2591 93 1056 1891
Fragments arranged by size:
2591 1891 1056 749 93
BmrI ACTGGGnnnn_n'
Cuts at: 1400 3123 3592 3673 4008 6152 1400
Size: 1723 469 81 335 2144 1628
Fragments arranged by size:
2144 1723 1628 469 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1554 4747 1554
Size: 3193 3187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 878 1803 1931 878
Size: 925 128 5327
Fragments arranged by size:
5327 925 128
Bpu10I CC'TnA_GC
Cuts at: 1259 2188 1259
Size: 929 5451
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1325 1842 3556 4435 4927 5225 1325
Size: 517 1714 879 492 298 2480
Fragments arranged by size:
2480 1714 879 517 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1585 2919 3295 6084 1585
Size: 1334 376 2789 1881
Fragments arranged by size:
2789 1881 1334 376
BsaAI yAC'GTr
Cuts at: 3733 3733
Size: 6380
BsaBI GATnn'nnATC
Cuts at: 6 1720 3894 5931 6
Size: 1714 2174 2037 455
Fragments arranged by size:
2174 2037 1714 455
BsaHI Gr'CG_yC
Cuts at: 3811 4059 4761 6257 3811
Size: 248 702 1496 3934
Fragments arranged by size:
3934 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2846 3569 4090 4768 5042 5189 5600 2846
Size: 723 521 678 274 147 411 3626
Fragments arranged by size:
3626 723 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2138 2168 2138
Size: 30 6350
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1603 3211 3214 1603
Size: 1608 3 4769
Fragments arranged by size:
4769 1608 3
BseYI C'CCAG_C
Cuts at: 2088 3805 4563 5140 5636 2088
Size: 1717 758 577 496 2832
Fragments arranged by size:
2832 1717 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 2082 244
Size: 1838 4542
BsiEI CG_ry'CG
Cuts at: 104 3922 3968 5176 104
Size: 3818 46 1208 1308
Fragments arranged by size:
3818 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 307 1414 3394 4172 4362 5154 307
Size: 1107 1980 778 190 792 1533
Fragments arranged by size:
1980 1533 1107 792 778 190
BsmI GAATG_Cn'
Cuts at: 2753 2753
Size: 6380
BsmBI CGTCTCn'nnnn_
Cuts at: 3253 3253
Size: 6380
Bsp1286I G_dGCh'C
Cuts at: 307 665 1414 3394 4005 4098 4172 4362 4424
Size: 358 749 1980 611 93 74 190 62
Cuts at: 4424 5154 307
Size: 730 1533
Fragments arranged by size:
1980 1533 749 730 611 358 190 93
74 62
BspEI T'CCGG_A
Cuts at: 5600 5600
Size: 6380
BspHI T'CATG_A
Cuts at: 1970 5556 1970
Size: 3586 2794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 621 1100 3430 3644 3946 4327 621
Size: 479 2330 214 302 381 2674
Fragments arranged by size:
2674 2330 479 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4672 4726 6210 6375 50
Size: 4622 54 1484 165 55
Fragments arranged by size:
4622 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4292 4292
Size: 6380
BsrFI r'CCGG_y
Cuts at: 1955 4378 4559 4768 1955
Size: 2423 181 209 3567
Fragments arranged by size:
3567 2423 209 181
BsrGI T'GTAC_A
Cuts at: 398 923 398
Size: 525 5855
BssSI C'ACGA_G
Cuts at: 4651 5009 4651
Size: 358 6022
BstAPI GCAn_nnn'nTGC
Cuts at: 3757 3757
Size: 6380
BstBI TT'CG_AA
Cuts at: 2647 4741 2647
Size: 2094 4286
BstEII G'GTnAC_C
Cuts at: 804 804
Size: 6380
BstXI CCAn_nnnn'nTGG
Cuts at: 918 1206 918
Size: 288 6092
BstYI r'GATC_y
Cuts at: 1 750 1532 2008 2606 2768 3405 3872 3895
Size: 749 782 476 598 162 637 467 23
Cuts at: 3895 4230 4476 5488 5574 5586 5932 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 782 749 637 598 476 467 449
346 335 246 162 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3194 3194
Size: 6380
BtgI C'CryG_G
Cuts at: 107 947 1380 1556 2000 107
Size: 840 433 176 444 4487
Fragments arranged by size:
4487 840 444 433 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1581 1620 4330 4511 4674 1581
Size: 39 2710 181 163 3287
Fragments arranged by size:
3287 2710 181 163 39
BtsI GCAGTG_nn'
Cuts at: 378 1988 3623 378
Size: 1610 1635 3135
Fragments arranged by size:
3135 1635 1610
DraI TTT'AAA
Cuts at: 731 3379 731
Size: 2648 3732
DraIII CAC_nnn'GTG
Cuts at: 2734 2734
Size: 6380
DrdI GACnn_nn'nnGTC
Cuts at: 2437 4086 4944 2437
Size: 1649 858 3873
Fragments arranged by size:
3873 1649 858
EaeI y'GGCC_r
Cuts at: 1275 1502 2003 2624 2852 3965 4139 4530 4557
Size: 227 501 621 228 1113 174 391 27
Cuts at: 4557 5902 1275
Size: 1345 1753
Fragments arranged by size:
1753 1345 1113 621 501 391 228 227
174 27
EagI C'GGCC_G
Cuts at: 3965 3965
Size: 6380
EarI CTCTTCn'nnn_
Cuts at: 293 425 1309 2917 3013 3239 3351 4403 4613
Size: 132 884 1608 96 226 112 1052 210
Cuts at: 4613 293
Size: 2060
Fragments arranged by size:
2060 1608 1052 884 226 210 132 112
96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4588 4898 5044 6190 4588
Size: 310 146 1146 4778
Fragments arranged by size:
4778 1146 310 146
EcoICRI GAG'CTC
Cuts at: 305 3392 305
Size: 3087 3293
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 878 1053 1439 1669 1803 1931 2062 3037
Size: 564 175 386 230 134 128 131 975
Cuts at: 3037 3127 3263 3332 4205 4637 5384 5652 314
Size: 90 136 69 873 432 747 268 1042
Fragments arranged by size:
1042 975 873 747 564 432 386 268
230 175 136 134 131 128 90 69
EcoO109I rG'GnC_Cy
Cuts at: 647 661 662 2110 3204 3519 647
Size: 14 1 1448 1094 315 3508
Fragments arranged by size:
3508 1448 1094 315 14 1
EcoRI G'AATT_C
Cuts at: 231 3384 231
Size: 3153 3227
EcoRV GAT'ATC
Cuts at: 1148 2226 1148
Size: 1078 5302
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4399 4399
Size: 6380
FspI TGC'GCA
Cuts at: 4161 4161
Size: 6380
HaeII r_GCGC'y
Cuts at: 3149 3814 4062 5084 6063 3149
Size: 665 248 1022 979 3466
Fragments arranged by size:
3466 1022 979 665 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 105 137 1167 1199 2037 2069 2138 2170 2449
Size: 32 1030 32 838 32 69 32 279
Cuts at: 2449 2481 2728 2760 3012 3044 4222 4252 4254
Size: 32 247 32 252 32 1178 30 2
Cuts at: 4254 4284 105
Size: 30 2201
Fragments arranged by size:
2201 1178 1030 838 279 252 247 69
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 837 1089 1337 2157 3419 837
Size: 252 248 820 1262 3798
Fragments arranged by size:
3798 1262 820 252 248
HpaI GTT'AAC
Cuts at: 1337 1337
Size: 6380
KasI G'GCGC_C
Cuts at: 3810 4058 3810
Size: 248 6132
KpnI G_GTAC'C
Cuts at: 613 2847 3400 613
Size: 2234 553 3593
Fragments arranged by size:
3593 2234 553
MluI A'CGCG_T
Cuts at: 5813 5813
Size: 6380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1058 2396 5051 5235 1058
Size: 1338 2655 184 2203
Fragments arranged by size:
2655 2203 1338 184
MscI TGG'CCA
Cuts at: 1277 1504 2626 2854 4141 1277
Size: 227 1122 228 1287 3516
Fragments arranged by size:
3516 1287 1122 228 227
MslI CAynn'nnrTG
Cuts at: 466 596 883 1204 1600 2305 2806 2945 3049
Size: 130 287 321 396 705 501 139 104
Cuts at: 3049 3319 4369 4496 466
Size: 270 1050 127 2350
Fragments arranged by size:
2350 1050 705 501 396 321 287 270
139 130 127 104
MspA1I CmG'CkG
Cuts at: 228 341 1417 1845 3491 3805 4165 5178 5423
Size: 113 1076 428 1646 314 360 1013 245
Cuts at: 5423 228
Size: 1185
Fragments arranged by size:
1646 1185 1076 1013 428 360 314 245
113
NaeI GCC'GGC
Cuts at: 4561 4561
Size: 6380
NarI GG'CG_CC
Cuts at: 3811 4059 3811
Size: 248 6132
NcoI C'CATG_G
Cuts at: 107 1380 107
Size: 1273 5107
NgoMIV G'CCGG_C
Cuts at: 4559 4559
Size: 6380
NsiI A_TGCA'T
Cuts at: 2642 3069 2642
Size: 427 5953
NspI r_CATG'y
Cuts at: 3048 3433 3933 4840 3048
Size: 385 500 907 4588
Fragments arranged by size:
4588 907 500 385
PciI A'CATG_T
Cuts at: 4836 4836
Size: 6380
PflMI CCAn_nnn'nTGG
Cuts at: 1879 1879
Size: 6380
PfoI T'CCnGG_A
Cuts at: 1489 1489
Size: 6380
PmeI GTTT'AAAC
Cuts at: 3379 3379
Size: 6380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5519 5551 5519
Size: 32 6348
PpuMI rG'GwC_Cy
Cuts at: 647 3204 647
Size: 2557 3823
PspOMI G'GGCC_C
Cuts at: 661 661
Size: 6380
PstI C_TGCA'G
Cuts at: 1698 3427 4112 1698
Size: 1729 685 3966
Fragments arranged by size:
3966 1729 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6380
PvuII CAG'CTG
Cuts at: 228 1417 1845 3805 4165 228
Size: 1189 428 1960 360 2443
Fragments arranged by size:
2443 1960 1189 428 360
RsrII CG'GwC_CG
Cuts at: 4575 4575
Size: 6380
SacI G_AGCT'C
Cuts at: 307 3394 307
Size: 3087 3293
SalI G'TCGA_C
Cuts at: 3417 3417
Size: 6380
SapI GCTCTTCn'nnn_
Cuts at: 1309 4403 4613 1309
Size: 3094 210 3076
Fragments arranged by size:
3094 3076 210
SbfI CC_TGCA'GG
Cuts at: 3427 3427
Size: 6380
ScaI AGT'ACT
Cuts at: 5897 5897
Size: 6380
SexAI A'CCwGG_T
Cuts at: 3353 3353
Size: 6380
SfcI C'TryA_G
Cuts at: 33 656 1694 3423 4108 5101 5292 33
Size: 623 1038 1729 685 993 191 1121
Fragments arranged by size:
1729 1121 1038 993 685 623 191
SfoI GGC'GCC
Cuts at: 3812 4060 3812
Size: 248 6132
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6380
SmaI CCC'GGG
Cuts at: 3402 3402
Size: 6380
SmlI C'TyrA_G
Cuts at: 1340 1857 3535 4450 4942 5204 1340
Size: 517 1678 915 492 262 2516
Fragments arranged by size:
2516 1678 915 517 492 262
SpeI A'CTAG_T
Cuts at: 67 1884 67
Size: 1817 4563
SphI G_CATG'C
Cuts at: 3048 3433 3933 3048
Size: 385 500 5495
Fragments arranged by size:
5495 500 385
SspI AAT'ATT
Cuts at: 2368 2368
Size: 6380
StuI AGG'CCT
Cuts at: 2474 2474
Size: 6380
StyI C'CwwG_G
Cuts at: 107 1380 1626 2778 3514 3868 107
Size: 1273 246 1152 736 354 2619
Fragments arranged by size:
2619 1273 1152 736 354 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2213 3963 4096 4555 4764 2213
Size: 1750 133 459 209 3829
Fragments arranged by size:
3829 1750 459 209 133
TatI w'GTAC_w
Cuts at: 398 923 5895 398
Size: 525 4972 883
Fragments arranged by size:
4972 883 525
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 552 584 1388 1416 1420 1448 1721 1753 2318
Size: 32 804 28 4 28 273 32 565
Cuts at: 2318 2350 2585 2617 5897 5929 552
Size: 32 235 32 3280 32 1003
Fragments arranged by size:
3280 1003 804 565 273 235 32 32
32 32 32 28 28 4
Tth111I GACn'n_nGTC
Cuts at: 1366 3187 4177 1366
Size: 1821 990 3569
Fragments arranged by size:
3569 1821 990
XbaI T'CTAG_A
Cuts at: 753 3411 753
Size: 2658 3722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1362 1387 3875 1362
Size: 25 2488 3867
Fragments arranged by size:
3867 2488 25
XmaI C'CCGG_G
Cuts at: 3400 3400
Size: 6380
XmnI GAAnn'nnTTC
Cuts at: 1045 1214 2999 1045
Size: 169 1785 4426
Fragments arranged by size:
4426 1785 169
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI AscI AvrII BbvCI
BmgBI BmtI BsiWI BssHII BstZ17I ClaI CspCI EcoNI
FseI FspAI HindIII MfeI NdeI NheI NotI NruI
PacI PasI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI