Restriction map for
pF1KE3374
(Circular) (Six-base) MAPSORT of: pF1KE3374.seq Check: 1994 from: 1 to: 6464
pF1KE3374 6464 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 611 2382 2457 611
Size: 1771 75 4618
Fragments arranged by size:
4618 1771 75
AatII G_ACGT'C
Cuts at: 2377 2377
Size: 6464
AccI GT'mk_AC
Cuts at: 3502 3502
Size: 6464
Acc65I G'GTAC_C
Cuts at: 3480 3480
Size: 6464
AclI AA'CG_TT
Cuts at: 6304 6304
Size: 6464
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1801 2010 4289 4721 5468 5736 1060
Size: 741 209 2279 432 747 268 1788
Fragments arranged by size:
2279 1788 747 741 432 268 209
AflIII A'CryG_T
Cuts at: 986 3816 4920 5897 986
Size: 2830 1104 977 1553
Fragments arranged by size:
2830 1553 1104 977
AgeI A'CCGG_T
Cuts at: 1994 4852 1994
Size: 2858 3606
AhdI GACnn_n'nnGTC
Cuts at: 2423 2423
Size: 6464
AleI CACnn'nnGTG
Cuts at: 754 2536 2701 754
Size: 1782 165 4517
Fragments arranged by size:
4517 1782 165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 760 2533 3044 3169 760
Size: 1773 511 125 4055
Fragments arranged by size:
4055 1773 511 125
AlwNI CAG_nnn'CTG
Cuts at: 154 417 561 867 1966 2232 2273 2805 3099
Size: 263 144 306 1099 266 41 532 294
Cuts at: 3099 3769 5336 154
Size: 670 1567 1282
Fragments arranged by size:
1567 1282 1099 670 532 306 294 266
263 144 41
ApaI G_GGCC'C
Cuts at: 309 411 897 1673 309
Size: 102 486 776 5100
Fragments arranged by size:
5100 776 486 102
ApaLI G'TGCA_C
Cuts at: 422 1084 1702 5234 422
Size: 662 618 3532 1652
Fragments arranged by size:
3532 1652 662 618
ApoI r'AATT_y
Cuts at: 1119 2092 3468 5817 6073 1119
Size: 973 1376 2349 256 1510
Fragments arranged by size:
2349 1510 1376 973 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6464
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6464
AvaI C'yCGr_G
Cuts at: 545 1954 2072 2350 3484 545
Size: 1409 118 278 1134 3525
Fragments arranged by size:
3525 1409 1134 278 118
BamHI G'GATC_C
Cuts at: 1406 1715 1751 3489 1406
Size: 309 36 1738 4381
Fragments arranged by size:
4381 1738 309 36
BanI G'GyrC_C
Cuts at: 144 254 517 550 662 724 736 776 1187
Size: 110 263 33 112 62 12 40 411
Cuts at: 1187 1984 2281 2592 3480 3894 4142 4177 144
Size: 797 297 311 888 414 248 35 2431
Fragments arranged by size:
2431 888 797 414 411 311 297 263
248 112 110 62 40 35 33 12
BanII G_rGCy'C
Cuts at: 309 411 897 1673 1949 2327 2483 2732 2768
Size: 102 486 776 276 378 156 249 36
Cuts at: 2768 2871 3478 4508 309
Size: 103 607 1030 2265
Fragments arranged by size:
2265 1030 776 607 486 378 276 249
156 103 102 36
BbeI G_GCGC'C
Cuts at: 258 554 666 780 1988 3898 4146 258
Size: 296 112 114 1208 1910 248 2576
Fragments arranged by size:
2576 1910 1208 296 248 114 112
BbsI GAAGACnn'nnnn_
Cuts at: 1641 2004 3337 1641
Size: 363 1333 4768
Fragments arranged by size:
4768 1333 363
BbvCI CC'TCA_GC
Cuts at: 667 667
Size: 6464
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1043 1077 2493 2527 1043
Size: 34 1416 34 4980
Fragments arranged by size:
4980 1416 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4362 5123 6464 4362
Size: 761 1341 4362
Fragments arranged by size:
4362 1341 761
BclI T'GATC_A
Cuts at: 3984 3984
Size: 6464
BglI GCCn_nnn'nGGC
Cuts at: 650 707 1217 2331 650
Size: 57 510 1114 4783
Fragments arranged by size:
4783 1114 510 57
BglII A'GATC_T
Cuts at: 3979 6016 3979
Size: 2037 4427
BlpI GC'TnA_GC
Cuts at: 1943 3576 1943
Size: 1633 4831
Bme1580I G_kGCm'C
Cuts at: 309 344 411 426 897 1088 1673 1706 2284
Size: 35 67 15 471 191 585 33 578
Cuts at: 2284 2698 2742 4089 4182 5238 309
Size: 414 44 1347 93 1056 1535
Fragments arranged by size:
1535 1347 1056 585 578 471 414 191
93 67 44 35 33 15
BmgBI CAC'GTC
Cuts at: 2049 2049
Size: 6464
BmrI ACTGGGnnnn_n'
Cuts at: 1203 1857 2235 3676 3757 4092 6236 1203
Size: 654 378 1441 81 335 2144 1431
Fragments arranged by size:
2144 1441 1431 654 378 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1380 4831 1380
Size: 3451 3013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 246 259 315 840 844 960 1129 1140
Size: 70 13 56 525 4 116 169 11
Cuts at: 1140 1533 1705 2077 2740 2798 3076 3424 176
Size: 393 172 372 663 58 278 348 3216
Fragments arranged by size:
3216 663 525 393 372 348 278 172
169 116 70 58 56 13 11 4
Bpu10I CC'TnA_GC
Cuts at: 667 1657 2930 3377 667
Size: 990 1273 447 3754
Fragments arranged by size:
3754 1273 990 447
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2656 3026 3640 4519 5011 5309 2656
Size: 370 614 879 492 298 3811
Fragments arranged by size:
3811 879 614 492 370 298
BsaI GGTCTCn'nnnn_
Cuts at: 973 1509 3244 6168 973
Size: 536 1735 2924 1269
Fragments arranged by size:
2924 1735 1269 536
BsaAI yAC'GTr
Cuts at: 1896 3817 1896
Size: 1921 4543
BsaBI GATnn'nnATC
Cuts at: 6 2917 3978 6015 6
Size: 2911 1061 2037 455
Fragments arranged by size:
2911 2037 1061 455
BsaHI Gr'CG_yC
Cuts at: 210 255 551 663 777 1209 1985 2042 2374
Size: 45 296 112 114 432 776 57 332
Cuts at: 2374 2831 3895 4143 4845 6341 210
Size: 457 1064 248 702 1496 333
Fragments arranged by size:
1496 1064 776 702 457 432 333 332
296 248 114 112 57 45
BsaWI w'CCGG_w
Cuts at: 923 1994 3653 4174 4852 5126 5273 5684 923
Size: 1071 1659 521 678 274 147 411 1703
Fragments arranged by size:
1703 1659 1071 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 225 228 475 478 585 1369 1516 1915 225
Size: 3 247 3 107 784 147 399 4774
Fragments arranged by size:
4774 784 399 247 147 107 3 3
BseYI C'CCAG_C
Cuts at: 175 223 269 410 554 1368 1928 2327 2691
Size: 48 46 141 144 814 560 399 364
Cuts at: 2691 2937 3109 3889 4647 5224 5720 175
Size: 246 172 780 758 577 496 919
Fragments arranged by size:
919 814 780 758 577 560 496 399
364 246 172 144 141 48 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 921 2485 2557 2560 2635 3017 3085 3139
Size: 290 1564 72 3 75 382 68 54
Cuts at: 3139 631
Size: 3956
Fragments arranged by size:
3956 1564 382 290 75 72 68 54
3
BsiEI CG_ry'CG
Cuts at: 104 806 1995 2865 4006 4052 5260 104
Size: 702 1189 870 1141 46 1208 1308
Fragments arranged by size:
1308 1208 1189 1141 870 702 46
BsiHKAI G_wGCw'C
Cuts at: 383 426 968 1088 1366 1706 2312 2441 2732
Size: 43 542 120 278 340 606 129 291
Cuts at: 2732 2879 3478 4256 4446 5238 383
Size: 147 599 778 190 792 1609
Fragments arranged by size:
1609 792 778 606 599 542 340 291
278 190 147 129 120 43
BsmBI CGTCTCn'nnnn_
Cuts at: 3125 3442 3125
Size: 317 6147
Bsp1286I G_dGCh'C
Cuts at: 309 344 383 411 426 897 968 1088 1366
Size: 35 39 28 15 471 71 120 278
Cuts at: 1366 1673 1706 1949 2284 2312 2327 2441 2483
Size: 307 33 243 335 28 15 114 42
Cuts at: 2483 2698 2732 2742 2768 2871 2879 3478 4089
Size: 215 34 10 26 103 8 599 611
Cuts at: 4089 4182 4256 4446 4508 5238 309
Size: 93 74 190 62 730 1535
Fragments arranged by size:
1535 730 611 599 471 335 307 278
243 215 190 120 114 103 93 74
71 62 42 39 35 34 33 28
28 26 15 15 10 8
BspEI T'CCGG_A
Cuts at: 923 5684 923
Size: 4761 1703
BspHI T'CATG_A
Cuts at: 1550 3326 5640 1550
Size: 1776 2314 2374
Fragments arranged by size:
2374 2314 1776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 590 611 1718 2382 2457 3206 3514 3728 4030
Size: 21 1107 664 75 749 308 214 302
Cuts at: 4030 4411 590
Size: 381 2643
Fragments arranged by size:
2643 1107 749 664 381 308 302 214
75 21
BsrBI CCG'CTC
Cuts at: 50 260 915 1548 2412 4756 4810 6294 6459
Size: 210 655 633 864 2344 54 1484 165
Cuts at: 6459 50
Size: 55
Fragments arranged by size:
2344 1484 864 655 633 210 165 55
54
BsrDI GCAATG_nn'
Cuts at: 4376 4376
Size: 6464
BsrFI r'CCGG_y
Cuts at: 323 733 1994 2589 3410 4462 4643 4852 323
Size: 410 1261 595 821 1052 181 209 1935
Fragments arranged by size:
1935 1261 1052 821 595 410 209 181
BssSI C'ACGA_G
Cuts at: 1296 2065 4735 5093 1296
Size: 769 2670 358 2667
Fragments arranged by size:
2670 2667 769 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1710 2592 3293 3841 1710
Size: 882 701 548 4333
Fragments arranged by size:
4333 882 701 548
BstBI TT'CG_AA
Cuts at: 4825 4825
Size: 6464
BstXI CCAn_nnnn'nTGG
Cuts at: 183 697 2172 183
Size: 514 1475 4475
Fragments arranged by size:
4475 1475 514
BstYI r'GATC_y
Cuts at: 1 920 1406 1715 1751 1883 2222 3221 3489
Size: 919 486 309 36 132 339 999 268
Cuts at: 3489 3956 3979 4314 4560 5572 5658 5670 6016
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6016 1
Size: 449
Fragments arranged by size:
1012 999 919 486 467 449 346 339
335 309 268 246 132 86 36 23
12
Bsu36I CC'TnA_GG
Cuts at: 684 720 1499 1967 684
Size: 36 779 468 5181
Fragments arranged by size:
5181 779 468 36
BtgI C'CryG_G
Cuts at: 107 401 611 1385 2534 107
Size: 294 210 774 1149 4037
Fragments arranged by size:
4037 1149 774 294 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2496 4414 4595 4758 2496
Size: 1918 181 163 4202
Fragments arranged by size:
4202 1918 181 163
BtsI GCAGTG_nn'
Cuts at: 2428 2837 3016 3707 2428
Size: 409 179 691 5185
Fragments arranged by size:
5185 691 409 179
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1833 1868 1833
Size: 35 6429
DraI TTT'AAA
Cuts at: 3463 3463
Size: 6464
DrdI GACnn_nn'nnGTC
Cuts at: 987 2047 4170 5028 987
Size: 1060 2123 858 2423
Fragments arranged by size:
2423 2123 1060 858
EaeI y'GGCC_r
Cuts at: 803 1283 2174 2381 3392 4049 4223 4614 4641
Size: 480 891 207 1011 657 174 391 27
Cuts at: 4641 5986 803
Size: 1345 1281
Fragments arranged by size:
1345 1281 1011 891 657 480 391 207
174 27
EagI C'GGCC_G
Cuts at: 803 4049 803
Size: 3246 3218
EarI CTCTTCn'nnn_
Cuts at: 708 1453 3044 4487 4697 708
Size: 745 1591 1443 210 2475
Fragments arranged by size:
2475 1591 1443 745 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 869 2414 2571 4672 4982 5128 6274 869
Size: 1545 157 2101 310 146 1146 1059
Fragments arranged by size:
2101 1545 1146 1059 310 157 146
EcoICRI GAG'CTC
Cuts at: 2730 3476 2730
Size: 746 5718
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 246 259 315 840 844 960 1060 1129
Size: 70 13 56 525 4 116 100 69
Cuts at: 1129 1140 1533 1705 1801 2010 2077 2740 2798
Size: 11 393 172 96 209 67 663 58
Cuts at: 2798 3076 3424 4289 4721 5468 5736 176
Size: 278 348 865 432 747 268 904
Fragments arranged by size:
904 865 747 663 525 432 393 348
278 268 209 172 116 100 96 70
69 67 58 56 13 11 4
EcoNI CCTnn'n_nnAGG
Cuts at: 1473 1660 1473
Size: 187 6277
EcoO109I rG'GnC_Cy
Cuts at: 151 305 407 642 893 894 1424 1495 1669
Size: 154 102 235 251 1 530 71 174
Cuts at: 1669 1932 1963 2169 2229 2646 3317 3603 151
Size: 263 31 206 60 417 671 286 3012
Fragments arranged by size:
3012 671 530 417 286 263 251 235
206 174 154 102 71 60 31 1
EcoRI G'AATT_C
Cuts at: 3468 3468
Size: 6464
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1155 1278 2838 4483 1155
Size: 123 1560 1645 3136
Fragments arranged by size:
3136 1645 1560 123
FspI TGC'GCA
Cuts at: 1065 2521 2608 4245 1065
Size: 1456 87 1637 3284
Fragments arranged by size:
3284 1637 1456 87
HaeII r_GCGC'y
Cuts at: 258 269 330 506 554 666 780 827 1684
Size: 11 61 176 48 112 114 47 857
Cuts at: 1684 1988 2564 3898 4146 5168 6147 258
Size: 304 576 1334 248 1022 979 575
Fragments arranged by size:
1334 1022 979 857 576 575 304 248
176 114 112 61 48 47 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1508 1540 1861 1893 2409 2441 4306 4336 4338
Size: 32 321 32 516 32 1865 30 2
Cuts at: 4338 4368 1508
Size: 30 3604
Fragments arranged by size:
3604 1865 516 321 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 437 3503 437
Size: 3066 3398
HindIII A'AGCT_T
Cuts at: 2488 2488
Size: 6464
KasI G'GCGC_C
Cuts at: 254 550 662 776 1984 3894 4142 254
Size: 296 112 114 1208 1910 248 2576
Fragments arranged by size:
2576 1910 1208 296 248 114 112
KpnI G_GTAC'C
Cuts at: 3484 3484
Size: 6464
MluI A'CGCG_T
Cuts at: 5897 5897
Size: 6464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5135 5319 5135
Size: 184 6280
MscI TGG'CCA
Cuts at: 1285 2176 4225 1285
Size: 891 2049 3524
Fragments arranged by size:
3524 2049 891
MslI CAynn'nnrTG
Cuts at: 754 2188 2461 2536 2614 2639 2701 4453 4580
Size: 1434 273 75 78 25 62 1752 127
Cuts at: 4580 754
Size: 2638
Fragments arranged by size:
2638 1752 1434 273 127 78 75 62
25
MspA1I CmG'CkG
Cuts at: 171 223 252 561 613 675 1107 1127 1292
Size: 52 29 309 52 62 432 20 165
Cuts at: 1292 1511 1920 1928 2331 2691 3271 3363 3435
Size: 219 409 8 403 360 580 92 72
Cuts at: 3435 3575 3889 4249 5262 5507 171
Size: 140 314 360 1013 245 1128
Fragments arranged by size:
1128 1013 580 432 409 403 360 360
314 309 245 219 165 140 92 72
62 52 52 29 20 8
NaeI GCC'GGC
Cuts at: 325 735 4645 325
Size: 410 3910 2144
Fragments arranged by size:
3910 2144 410
NarI GG'CG_CC
Cuts at: 255 551 663 777 1985 3895 4143 255
Size: 296 112 114 1208 1910 248 2576
Fragments arranged by size:
2576 1910 1208 296 248 114 112
NcoI C'CATG_G
Cuts at: 107 2534 107
Size: 2427 4037
NdeI CA'TA_TG
Cuts at: 957 957
Size: 6464
NgoMIV G'CCGG_C
Cuts at: 323 733 4643 323
Size: 410 3910 2144
Fragments arranged by size:
3910 2144 410
NruI TCG'CGA
Cuts at: 835 2429 835
Size: 1594 4870
NspI r_CATG'y
Cuts at: 2634 2798 3517 4017 4924 2634
Size: 164 719 500 907 4174
Fragments arranged by size:
4174 907 719 500 164
PasI CC'CwG_GG
Cuts at: 309 703 889 1812 2291 2302 3276 309
Size: 394 186 923 479 11 974 3497
Fragments arranged by size:
3497 974 923 479 394 186 11
PciI A'CATG_T
Cuts at: 4920 4920
Size: 6464
PflMI CCAn_nnn'nTGG
Cuts at: 154 417 867 2291 154
Size: 263 450 1424 4327
Fragments arranged by size:
4327 1424 450 263
PfoI T'CCnGG_A
Cuts at: 473 1156 2225 2825 2921 3092 473
Size: 683 1069 600 96 171 3845
Fragments arranged by size:
3845 1069 683 600 171 96
PmeI GTTT'AAAC
Cuts at: 3463 3463
Size: 6464
PmlI CAC'GTG
Cuts at: 1896 1896
Size: 6464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5603 5635 5603
Size: 32 6432
PpuMI rG'GwC_Cy
Cuts at: 1932 2169 2229 1932
Size: 237 60 6167
Fragments arranged by size:
6167 237 60
PspOMI G'GGCC_C
Cuts at: 305 407 893 1669 305
Size: 102 486 776 5100
Fragments arranged by size:
5100 776 486 102
PspXI vC'TCGA_Gb
Cuts at: 2350 2350
Size: 6464
PstI C_TGCA'G
Cuts at: 1346 2393 3511 4196 1346
Size: 1047 1118 685 3614
Fragments arranged by size:
3614 1118 1047 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6464
PvuII CAG'CTG
Cuts at: 1107 1292 1920 3889 4249 1107
Size: 185 628 1969 360 3322
Fragments arranged by size:
3322 1969 628 360 185
RsrII CG'GwC_CG
Cuts at: 4659 4659
Size: 6464
SacI G_AGCT'C
Cuts at: 2732 3478 2732
Size: 746 5718
SacII CC_GC'GG
Cuts at: 614 614
Size: 6464
SalI G'TCGA_C
Cuts at: 3501 3501
Size: 6464
SapI GCTCTTCn'nnn_
Cuts at: 708 3044 4487 4697 708
Size: 2336 1443 210 2475
Fragments arranged by size:
2475 2336 1443 210
SbfI CC_TGCA'GG
Cuts at: 3511 3511
Size: 6464
ScaI AGT'ACT
Cuts at: 1021 5981 1021
Size: 4960 1504
SfcI C'TryA_G
Cuts at: 33 1342 2389 2853 3507 4192 5185 5376 33
Size: 1309 1047 464 654 685 993 191 1121
Fragments arranged by size:
1309 1121 1047 993 685 654 464 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 707 707
Size: 6464
SfoI GGC'GCC
Cuts at: 256 552 664 778 1986 3896 4144 256
Size: 296 112 114 1208 1910 248 2576
Fragments arranged by size:
2576 1910 1208 296 248 114 112
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6464
SgrAI Cr'CCGG_yG
Cuts at: 323 323
Size: 6464
SmaI CCC'GGG
Cuts at: 3486 3486
Size: 6464
SmlI C'TyrA_G
Cuts at: 2072 2350 2671 3041 3619 4534 5026 5288 2072
Size: 278 321 370 578 915 492 262 3248
Fragments arranged by size:
3248 915 578 492 370 321 278 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6464
SphI G_CATG'C
Cuts at: 2634 3517 4017 2634
Size: 883 500 5081
Fragments arranged by size:
5081 883 500
StuI AGG'CCT
Cuts at: 232 817 2680 232
Size: 585 1863 4016
Fragments arranged by size:
4016 1863 585
StyI C'CwwG_G
Cuts at: 107 1673 2165 2450 2534 3598 3952 107
Size: 1566 492 285 84 1064 354 2619
Fragments arranged by size:
2619 1566 1064 492 354 285 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 409 1988 2879 3018 4047 4180 4639 4848 409
Size: 1579 891 139 1029 133 459 209 2025
Fragments arranged by size:
2025 1579 1029 891 459 209 139 133
TatI w'GTAC_w
Cuts at: 928 1019 5979 928
Size: 91 4960 1413
Fragments arranged by size:
4960 1413 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1207 1239 5981 6013 1207
Size: 32 4742 32 1658
Fragments arranged by size:
4742 1658 32 32
Tth111I GACn'n_nGTC
Cuts at: 4261 4261
Size: 6464
XbaI T'CTAG_A
Cuts at: 3495 3495
Size: 6464
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1680 1809 2376 3959 1680
Size: 129 567 1583 4185
Fragments arranged by size:
4185 1583 567 129
XhoI C'TCGA_G
Cuts at: 2072 2350 2072
Size: 278 6186
XmaI C'CCGG_G
Cuts at: 3484 3484
Size: 6464
XmnI GAAnn'nnTTC
Cuts at: 1243 3079 1243
Size: 1836 4628
ZraI GAC'GTC
Cuts at: 2375 2375
Size: 6464
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NruI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AflII AloI AscI AvrII BaeI BfrBI BmtI
BsaXI BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI
DraIII EcoRV FseI FspAI HpaI MfeI NheI NotI
NsiI PacI PshAI PsiI PsrI SanDI SexAI SnaBI
SrfI SspI SwaI