Restriction map for
pF1KE3378
(Circular) (Six-base) MAPSORT of: pF1KE3378.seq Check: 9288 from: 1 to: 6503
pF1KE3378 6503 bp
With 182 enzymes: SgfI *
June 11, 2014 14:42 ..
AccI GT'mk_AC
Cuts at: 2079 3541 2079
Size: 1462 5041
Acc65I G'GTAC_C
Cuts at: 3519 3519
Size: 6503
AclI AA'CG_TT
Cuts at: 6343 6343
Size: 6503
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 460 1769 2402 2649 2800 2885 3065 4328
Size: 74 1309 633 247 151 85 180 1263
Cuts at: 4328 4760 5507 5775 386
Size: 432 747 268 1114
Fragments arranged by size:
1309 1263 1114 747 633 432 268 247
180 151 85 74
AfeI AGC'GCT
Cuts at: 934 934
Size: 6503
AflIII A'CryG_T
Cuts at: 2043 3855 4959 5936 2043
Size: 1812 1104 977 2610
Fragments arranged by size:
2610 1812 1104 977
AgeI A'CCGG_T
Cuts at: 4891 4891
Size: 6503
AleI CACnn'nnGTG
Cuts at: 2895 2895
Size: 6503
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 578 578
Size: 6503
AlwNI CAG_nnn'CTG
Cuts at: 571 1567 3808 5375 571
Size: 996 2241 1567 1699
Fragments arranged by size:
2241 1699 1567 996
ApaLI G'TGCA_C
Cuts at: 5273 5273
Size: 6503
ApoI r'AATT_y
Cuts at: 206 225 316 347 384 514 1117 1494 2026
Size: 19 91 31 37 130 603 377 532
Cuts at: 2026 3219 3337 3507 5856 6112 206
Size: 1193 118 170 2349 256 597
Fragments arranged by size:
2349 1193 603 597 532 377 256 170
130 118 91 37 31 19
AseI AT'TA_AT
Cuts at: 20 584 2488 3413 20
Size: 564 1904 925 3110
Fragments arranged by size:
3110 1904 925 564
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6503
AvaI C'yCGr_G
Cuts at: 1440 3523 1440
Size: 2083 4420
BamHI G'GATC_C
Cuts at: 2748 3528 2748
Size: 780 5723
BanI G'GyrC_C
Cuts at: 1338 3519 3933 4181 4216 1338
Size: 2181 414 248 35 3625
Fragments arranged by size:
3625 2181 414 248 35
BanII G_rGCy'C
Cuts at: 3517 4547 3517
Size: 1030 5473
BbeI G_GCGC'C
Cuts at: 3937 4185 3937
Size: 248 6255
BbsI GAAGACnn'nnnn_
Cuts at: 750 750
Size: 6503
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 331 365 331
Size: 34 6469
BciVI GTATCCnnnnn_n'
Cuts at: 1658 2878 4401 5162 6503 1658
Size: 1220 1523 761 1341 1658
Fragments arranged by size:
1658 1523 1341 1220 761
BclI T'GATC_A
Cuts at: 3450 4023 3450
Size: 573 5930
BfrBI ATG'CAT
Cuts at: 3328 3328
Size: 6503
BglII A'GATC_T
Cuts at: 4018 6055 4018
Size: 2037 4466
BlpI GC'TnA_GC
Cuts at: 257 3615 257
Size: 3358 3145
Bme1580I G_kGCm'C
Cuts at: 4128 4221 5277 4128
Size: 93 1056 5354
Fragments arranged by size:
5354 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1425 1688 3715 3796 4131 6275 1425
Size: 263 2027 81 335 2144 1653
Fragments arranged by size:
2144 2027 1653 335 263 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 860 4870 860
Size: 4010 2493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1409 3138 1409
Size: 1729 4774
Bpu10I CC'TnA_GC
Cuts at: 251 3093 251
Size: 2842 3661
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 812 2000 2114 2739 3679 4558 5050 5348 812
Size: 1188 114 625 940 879 492 298 1967
Fragments arranged by size:
1967 1188 940 879 625 492 298 114
BsaI GGTCTCn'nnnn_
Cuts at: 1131 6207 1131
Size: 5076 1427
BsaAI yAC'GTr
Cuts at: 957 3856 957
Size: 2899 3604
BsaBI GATnn'nnATC
Cuts at: 6 4017 6054 6
Size: 4011 2037 455
Fragments arranged by size:
4011 2037 455
BsaHI Gr'CG_yC
Cuts at: 3934 4182 4884 6380 3934
Size: 248 702 1496 4057
Fragments arranged by size:
4057 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3692 4213 4891 5165 5312 5723 3692
Size: 521 678 274 147 411 4472
Fragments arranged by size:
4472 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 683 713 861 882 891 912 946 976 1566
Size: 30 148 21 9 21 34 30 590
Cuts at: 1566 1596 2901 2931 683
Size: 30 1305 30 4255
Fragments arranged by size:
4255 1305 590 148 34 30 30 30
30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 129 836 2003 129
Size: 707 1167 4629
Fragments arranged by size:
4629 1167 707
BseYI C'CCAG_C
Cuts at: 3928 4686 5263 5759 3928
Size: 758 577 496 4672
Fragments arranged by size:
4672 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 1783 2570 2640 2786 631
Size: 1152 787 70 146 4348
Fragments arranged by size:
4348 1152 787 146 70
BsiEI CG_ry'CG
Cuts at: 104 4045 4091 5299 104
Size: 3941 46 1208 1308
Fragments arranged by size:
3941 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3517 4295 4485 5277 3517
Size: 778 190 792 4743
Fragments arranged by size:
4743 792 778 190
BsmI GAATG_Cn'
Cuts at: 1151 3328 1151
Size: 2177 4326
Bsp1286I G_dGCh'C
Cuts at: 3517 4128 4221 4295 4485 4547 5277 3517
Size: 611 93 74 190 62 730 4743
Fragments arranged by size:
4743 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 5723 5723
Size: 6503
BspHI T'CATG_A
Cuts at: 1175 5679 1175
Size: 4504 1999
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3553 3767 4069 4450 3553
Size: 214 302 381 5606
Fragments arranged by size:
5606 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2793 4795 4849 6333 6498 50
Size: 2743 2002 54 1484 165 55
Fragments arranged by size:
2743 2002 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4415 4415
Size: 6503
BsrFI r'CCGG_y
Cuts at: 188 4501 4682 4891 188
Size: 4313 181 209 1800
Fragments arranged by size:
4313 1800 209 181
BssSI C'ACGA_G
Cuts at: 4774 5132 4774
Size: 358 6145
BstAPI GCAn_nnn'nTGC
Cuts at: 571 3880 571
Size: 3309 3194
BstBI TT'CG_AA
Cuts at: 351 2316 2337 4864 351
Size: 1965 21 2527 1990
Fragments arranged by size:
2527 1990 1965 21
BstEII G'GTnAC_C
Cuts at: 2701 2701
Size: 6503
BstYI r'GATC_y
Cuts at: 1 288 540 757 1285 2748 3528 3995 4018
Size: 287 252 217 528 1463 780 467 23
Cuts at: 4018 4353 4599 5611 5697 5709 6055 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1463 1012 780 528 467 449 346 335
287 252 246 217 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 220 1706 220
Size: 1486 5017
BtgI C'CryG_G
Cuts at: 2710 2710
Size: 6503
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2832 4453 4634 4797 2832
Size: 1621 181 163 4538
Fragments arranged by size:
4538 1621 181 163
BtsI GCAGTG_nn'
Cuts at: 1452 1565 3746 1452
Size: 113 2181 4209
Fragments arranged by size:
4209 2181 113
DraI TTT'AAA
Cuts at: 2025 3138 3502 2025
Size: 1113 364 5026
Fragments arranged by size:
5026 1113 364
DrdI GACnn_nn'nnGTC
Cuts at: 4209 5067 4209
Size: 858 5645
EaeI y'GGCC_r
Cuts at: 4088 4262 4653 4680 6025 4088
Size: 174 391 27 1345 4566
Fragments arranged by size:
4566 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4088 4088
Size: 6503
EarI CTCTTCn'nnn_
Cuts at: 106 479 1973 2318 3017 4526 4736 106
Size: 373 1494 345 699 1509 210 1873
Fragments arranged by size:
1873 1509 1494 699 373 345 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4711 5021 5167 6313 4711
Size: 310 146 1146 4901
Fragments arranged by size:
4901 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3515 3515
Size: 6503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 460 1409 1769 2402 2649 2800 2885 3065
Size: 74 949 360 633 247 151 85 180
Cuts at: 3065 3138 4328 4760 5507 5775 386
Size: 73 1190 432 747 268 1114
Fragments arranged by size:
1190 1114 949 747 633 432 360 268
247 180 151 85 74 73
EcoNI CCTnn'n_nnAGG
Cuts at: 860 881 1049 3348 860
Size: 21 168 2299 4015
Fragments arranged by size:
4015 2299 168 21
EcoO109I rG'GnC_Cy
Cuts at: 3079 3642 3079
Size: 563 5940
EcoRI G'AATT_C
Cuts at: 514 3507 514
Size: 2993 3510
EcoRV GAT'ATC
Cuts at: 2373 2373
Size: 6503
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2347 4522 2347
Size: 2175 4328
FspI TGC'GCA
Cuts at: 4284 4284
Size: 6503
HaeII r_GCGC'y
Cuts at: 936 3937 4185 5207 6186 936
Size: 3001 248 1022 979 1253
Fragments arranged by size:
3001 1253 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 861 893 1079 1111 3421 3453 3462 3494 4345
Size: 32 186 32 2310 32 9 32 851
Cuts at: 4345 4375 4377 4407 861
Size: 30 2 30 2957
Fragments arranged by size:
2957 2310 851 186 32 32 32 32
30 30 9 2
HincII GTy'rAC
Cuts at: 281 624 3542 281
Size: 343 2918 3242
Fragments arranged by size:
3242 2918 343
HindIII A'AGCT_T
Cuts at: 1863 3047 1863
Size: 1184 5319
HpaI GTT'AAC
Cuts at: 624 624
Size: 6503
KasI G'GCGC_C
Cuts at: 3933 4181 3933
Size: 248 6255
KpnI G_GTAC'C
Cuts at: 3523 3523
Size: 6503
MfeI C'AATT_G
Cuts at: 509 3143 509
Size: 2634 3869
MluI A'CGCG_T
Cuts at: 5936 5936
Size: 6503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2100 3419 5174 5358 2100
Size: 1319 1755 184 3245
Fragments arranged by size:
3245 1755 1319 184
MscI TGG'CCA
Cuts at: 4264 4264
Size: 6503
MslI CAynn'nnrTG
Cuts at: 1187 1483 2895 4492 4619 1187
Size: 296 1412 1597 127 3071
Fragments arranged by size:
3071 1597 1412 296 127
MspA1I CmG'CkG
Cuts at: 571 3614 3928 4288 5301 5546 571
Size: 3043 314 360 1013 245 1528
Fragments arranged by size:
3043 1528 1013 360 314 245
NaeI GCC'GGC
Cuts at: 190 4684 190
Size: 4494 2009
NarI GG'CG_CC
Cuts at: 3934 4182 3934
Size: 248 6255
NgoMIV G'CCGG_C
Cuts at: 188 4682 188
Size: 4494 2009
NruI TCG'CGA
Cuts at: 3482 3482
Size: 6503
NsiI A_TGCA'T
Cuts at: 3330 3330
Size: 6503
NspI r_CATG'y
Cuts at: 2047 3328 3556 4056 4963 2047
Size: 1281 228 500 907 3587
Fragments arranged by size:
3587 1281 907 500 228
PciI A'CATG_T
Cuts at: 2043 4959 2043
Size: 2916 3587
PmeI GTTT'AAAC
Cuts at: 3502 3502
Size: 6503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5642 5674 5642
Size: 32 6471
PpuMI rG'GwC_Cy
Cuts at: 3079 3079
Size: 6503
PshAI GACnn'nnGTC
Cuts at: 750 3171 750
Size: 2421 4082
PstI C_TGCA'G
Cuts at: 567 3550 4235 567
Size: 2983 685 2835
Fragments arranged by size:
2983 2835 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6503
PvuII CAG'CTG
Cuts at: 571 3928 4288 571
Size: 3357 360 2786
Fragments arranged by size:
3357 2786 360
RsrII CG'GwC_CG
Cuts at: 4698 4698
Size: 6503
SacI G_AGCT'C
Cuts at: 3517 3517
Size: 6503
SalI G'TCGA_C
Cuts at: 3540 3540
Size: 6503
SapI GCTCTTCn'nnn_
Cuts at: 479 1973 4526 4736 479
Size: 1494 2553 210 2246
Fragments arranged by size:
2553 2246 1494 210
SbfI CC_TGCA'GG
Cuts at: 3550 3550
Size: 6503
ScaI AGT'ACT
Cuts at: 399 2689 6020 399
Size: 2290 3331 882
Fragments arranged by size:
3331 2290 882
SfcI C'TryA_G
Cuts at: 33 563 1630 3546 4231 5224 5415 33
Size: 530 1067 1916 685 993 191 1121
Fragments arranged by size:
1916 1121 1067 993 685 530 191
SfoI GGC'GCC
Cuts at: 3935 4183 3935
Size: 248 6255
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6503
SmaI CCC'GGG
Cuts at: 3525 3525
Size: 6503
SmlI C'TyrA_G
Cuts at: 827 2015 2129 2754 3658 4573 5065 5327 827
Size: 1188 114 625 904 915 492 262 2003
Fragments arranged by size:
2003 1188 915 904 625 492 262 114
SnaBI TAC'GTA
Cuts at: 957 957
Size: 6503
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6503
SphI G_CATG'C
Cuts at: 3556 4056 3556
Size: 500 6003
SspI AAT'ATT
Cuts at: 2607 2607
Size: 6503
StuI AGG'CCT
Cuts at: 150 150
Size: 6503
StyI C'CwwG_G
Cuts at: 1871 3637 3991 1871
Size: 1766 354 4383
Fragments arranged by size:
4383 1766 354
SwaI ATTT'AAAT
Cuts at: 2025 2025
Size: 6503
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 327 3218 4086 4219 4678 4887 327
Size: 2891 868 133 459 209 1943
Fragments arranged by size:
2891 1943 868 459 209 133
TatI w'GTAC_w
Cuts at: 397 2055 2687 6018 397
Size: 1658 632 3331 882
Fragments arranged by size:
3331 1658 882 632
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 683 715 791 823 2901 2933 6020 6052 683
Size: 32 76 32 2078 32 3087 32 1134
Fragments arranged by size:
3087 2078 1134 76 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4300 4300
Size: 6503
XbaI T'CTAG_A
Cuts at: 3534 3534
Size: 6503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 989 3998 989
Size: 3009 3494
XmaI C'CCGG_G
Cuts at: 3523 3523
Size: 6503
XmnI GAAnn'nnTTC
Cuts at: 1716 2561 1716
Size: 845 5658
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NruI NsiI NspI PciI PmeI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BglI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstXI BstZ17I ClaI CspCI DraIII FseI FspAI
NcoI NdeI NheI NotI PacI PasI PflMI PfoI
PmlI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI XhoI ZraI