Restriction map for
pF1KE3379
(Circular) (Six-base) MAPSORT of: pF1KE3379.seq Check: 1499 from: 1 to: 6503
pF1KE3379 6503 bp
With 182 enzymes: SgfI *
June 16, 2011 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3473 3473
Size: 6503
AatII G_ACGT'C
Cuts at: 1057 3344 1057
Size: 2287 4216
AccI GT'mk_AC
Cuts at: 3541 3541
Size: 6503
Acc65I G'GTAC_C
Cuts at: 2480 3519 2480
Size: 1039 5464
AclI AA'CG_TT
Cuts at: 6343 6343
Size: 6503
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 2506 2815 2902 3169 4328 4760 5507 5775
Size: 1914 309 87 267 1159 432 747 268
Cuts at: 5775 592
Size: 1320
Fragments arranged by size:
1914 1320 1159 747 432 309 268 267
87
AflIII A'CryG_T
Cuts at: 1148 2643 3855 4959 5936 1148
Size: 1495 1212 1104 977 1715
Fragments arranged by size:
1715 1495 1212 1104 977
AgeI A'CCGG_T
Cuts at: 4891 4891
Size: 6503
AhdI GACnn_n'nnGTC
Cuts at: 614 2029 3321 614
Size: 1415 1292 3796
Fragments arranged by size:
3796 1415 1292
AleI CACnn'nnGTG
Cuts at: 604 604
Size: 6503
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3401 3401
Size: 6503
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2128 2160 2916 2948 2128
Size: 32 756 32 5683
Fragments arranged by size:
5683 756 32 32
AlwNI CAG_nnn'CTG
Cuts at: 991 1509 1776 2526 3072 3808 5375 991
Size: 518 267 750 546 736 1567 2119
Fragments arranged by size:
2119 1567 750 736 546 518 267
ApaI G_GGCC'C
Cuts at: 194 1528 2076 2265 3004 3375 194
Size: 1334 548 189 739 371 3322
Fragments arranged by size:
3322 1334 739 548 371 189
ApaLI G'TGCA_C
Cuts at: 5273 5273
Size: 6503
ApoI r'AATT_y
Cuts at: 2567 3507 5856 6112 2567
Size: 940 2349 256 2958
Fragments arranged by size:
2958 2349 940 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6503
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6503
AvaI C'yCGr_G
Cuts at: 1028 1754 2068 2428 3523 1028
Size: 726 314 360 1095 4008
Fragments arranged by size:
4008 1095 726 360 314
BamHI G'GATC_C
Cuts at: 3041 3528 3041
Size: 487 6016
BanI G'GyrC_C
Cuts at: 885 1156 1659 2480 2617 3199 3519 3933 4181
Size: 271 503 821 137 582 320 414 248
Cuts at: 4181 4216 885
Size: 35 3172
Fragments arranged by size:
3172 821 582 503 414 320 271 248
137 35
BanII G_rGCy'C
Cuts at: 155 194 299 596 827 1166 1528 1673 1861
Size: 39 105 297 231 339 362 145 188
Cuts at: 1861 1890 2076 2265 2306 2424 2594 2960 3004
Size: 29 186 189 41 118 170 366 44
Cuts at: 3004 3375 3517 4547 155
Size: 371 142 1030 2111
Fragments arranged by size:
2111 1030 371 366 362 339 297 231
189 188 186 170 145 142 118 105
44 41 39 29
BbeI G_GCGC'C
Cuts at: 3937 4185 3937
Size: 248 6255
BbsI GAAGACnn'nnnn_
Cuts at: 414 1443 2900 2948 414
Size: 1029 1457 48 3969
Fragments arranged by size:
3969 1457 1029 48
BbvCI CC'TCA_GC
Cuts at: 1301 1301
Size: 6503
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6469
BciVI GTATCCnnnnn_n'
Cuts at: 4401 5162 6503 4401
Size: 761 1341 4401
Fragments arranged by size:
4401 1341 761
BclI T'GATC_A
Cuts at: 4023 4023
Size: 6503
BglI GCCn_nnn'nGGC
Cuts at: 822 1178 2042 2095 2551 2560 2665 822
Size: 356 864 53 456 9 105 4660
Fragments arranged by size:
4660 864 456 356 105 53 9
BglII A'GATC_T
Cuts at: 4018 6055 4018
Size: 2037 4466
BlpI GC'TnA_GC
Cuts at: 590 3253 3615 590
Size: 2663 362 3478
Fragments arranged by size:
3478 2663 362
Bme1580I G_kGCm'C
Cuts at: 194 890 1528 2076 2265 2620 3004 3375 4128
Size: 696 638 548 189 355 384 371 753
Cuts at: 4128 4221 5277 194
Size: 93 1056 1420
Fragments arranged by size:
1420 1056 753 696 638 548 384 371
355 189 93
BmgBI CAC'GTC
Cuts at: 2160 3282 2160
Size: 1122 5381
BmrI ACTGGGnnnn_n'
Cuts at: 908 1275 1431 2659 3715 3796 4131 6275 908
Size: 367 156 1228 1056 81 335 2144 1136
Fragments arranged by size:
2144 1228 1136 1056 367 335 156 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 318 711 1692 2443 3135 3317 3422 4870 318
Size: 393 981 751 692 182 105 1448 1951
Fragments arranged by size:
1951 1448 981 751 692 393 182 105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 299 460 729 733 843 932 1100 1237
Size: 67 161 269 4 110 89 168 137
Cuts at: 1237 1261 2002 2380 2548 3007 232
Size: 24 741 378 168 459 3728
Fragments arranged by size:
3728 741 459 378 269 168 168 161
137 110 89 67 24 4
Bpu10I CC'TnA_GC
Cuts at: 448 1112 1301 1777 1860 3247 448
Size: 664 189 476 83 1387 3704
Fragments arranged by size:
3704 1387 664 476 189 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2793 3679 4558 5050 5348 2793
Size: 886 879 492 298 3948
Fragments arranged by size:
3948 886 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 370 861 1092 1569 2812 6207 370
Size: 491 231 477 1243 3395 666
Fragments arranged by size:
3395 1243 666 491 477 231
BsaAI yAC'GTr
Cuts at: 1151 1649 1839 2580 3856 1151
Size: 498 190 741 1276 3798
Fragments arranged by size:
3798 1276 741 498 190
BsaBI GATnn'nnATC
Cuts at: 6 4017 6054 6
Size: 4011 2037 455
Fragments arranged by size:
4011 2037 455
BsaHI Gr'CG_yC
Cuts at: 1054 1790 1997 3341 3433 3934 4182 4884 6380
Size: 736 207 1344 92 501 248 702 1496
Cuts at: 6380 1054
Size: 1177
Fragments arranged by size:
1496 1344 1177 736 702 501 248 207
92
BsaWI w'CCGG_w
Cuts at: 3312 3692 4213 4891 5165 5312 5723 3312
Size: 380 521 678 274 147 411 4092
Fragments arranged by size:
4092 678 521 411 380 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2128 2158 2576 2606 2128
Size: 30 418 30 6025
Fragments arranged by size:
6025 418 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 568 1236 1627 1726 1729 1888 2572 2857 568
Size: 668 391 99 3 159 684 285 4214
Fragments arranged by size:
4214 684 668 391 285 159 99 3
BseYI C'CCAG_C
Cuts at: 133 434 1000 1362 1396 2040 2106 2233 2383
Size: 301 566 362 34 644 66 127 150
Cuts at: 2383 2950 2997 3143 3374 3928 4686 5263 5759
Size: 567 47 146 231 554 758 577 496
Cuts at: 5759 133
Size: 877
Fragments arranged by size:
877 758 644 577 567 566 554 496
362 301 231 150 146 127 66 47
34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1225 1624 2069 2521 2932 1225
Size: 399 445 452 411 4796
Fragments arranged by size:
4796 452 445 411 399
BsiEI CG_ry'CG
Cuts at: 104 1336 1547 2601 4045 4091 5299 104
Size: 1232 211 1054 1444 46 1208 1308
Fragments arranged by size:
1444 1308 1232 1208 1054 211 46
BsiHKAI G_wGCw'C
Cuts at: 299 1049 1328 1861 1877 2306 2594 2960 3517
Size: 750 279 533 16 429 288 366 557
Cuts at: 3517 4295 4485 5277 299
Size: 778 190 792 1525
Fragments arranged by size:
1525 792 778 750 557 533 429 366
288 279 190 16
BsmBI CGTCTCn'nnnn_
Cuts at: 3409 3409
Size: 6503
Bsp1286I G_dGCh'C
Cuts at: 155 194 299 596 827 890 1049 1166 1328
Size: 39 105 297 231 63 159 117 162
Cuts at: 1328 1528 1673 1861 1877 1890 2076 2265 2306
Size: 200 145 188 16 13 186 189 41
Cuts at: 2306 2424 2594 2620 2960 3004 3375 3517 4128
Size: 118 170 26 340 44 371 142 611
Cuts at: 4128 4221 4295 4485 4547 5277 155
Size: 93 74 190 62 730 1381
Fragments arranged by size:
1381 730 611 371 340 297 231 200
190 189 188 186 170 162 159 145
142 118 117 105 93 74 63 62
44 41 39 26 16 13
BspEI T'CCGG_A
Cuts at: 3312 5723 3312
Size: 2411 4092
BspHI T'CATG_A
Cuts at: 5679 5679
Size: 6503
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1004 1023 3473 3553 3767 4069 4450 1004
Size: 19 2450 80 214 302 381 3057
Fragments arranged by size:
3057 2450 381 302 214 80 19
BsrBI CCG'CTC
Cuts at: 50 1136 1245 4795 4849 6333 6498 50
Size: 1086 109 3550 54 1484 165 55
Fragments arranged by size:
3550 1484 1086 165 109 55 54
BsrDI GCAATG_nn'
Cuts at: 1467 2254 4415 1467
Size: 787 2161 3555
Fragments arranged by size:
3555 2161 787
BsrFI r'CCGG_y
Cuts at: 953 1116 1328 1427 1656 1662 1696 1907 4501
Size: 163 212 99 229 6 34 211 2594
Cuts at: 4501 4682 4891 953
Size: 181 209 2565
Fragments arranged by size:
2594 2565 229 212 211 209 181 163
99 34 6
BsrGI T'GTAC_A
Cuts at: 3260 3260
Size: 6503
BssSI C'ACGA_G
Cuts at: 1346 1537 2548 2734 3302 4774 5132 1346
Size: 191 1011 186 568 1472 358 2717
Fragments arranged by size:
2717 1472 1011 568 358 191 186
BstAPI GCAn_nnn'nTGC
Cuts at: 1509 2469 3880 1509
Size: 960 1411 4132
Fragments arranged by size:
4132 1411 960
BstBI TT'CG_AA
Cuts at: 4864 4864
Size: 6503
BstXI CCAn_nnnn'nTGG
Cuts at: 186 186
Size: 6503
BstYI r'GATC_y
Cuts at: 1 1197 1506 3041 3528 3995 4018 4353 4599
Size: 1196 309 1535 487 467 23 335 246
Cuts at: 4599 5611 5697 5709 6055 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1535 1196 1012 487 467 449 346 335
309 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 776 776
Size: 6503
BtgI C'CryG_G
Cuts at: 179 819 876 930 1817 2203 179
Size: 640 57 54 887 386 4479
Fragments arranged by size:
4479 887 640 386 57 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 242 2188 3439 4453 4634 4797 242
Size: 1946 1251 1014 181 163 1948
Fragments arranged by size:
1948 1946 1251 1014 181 163
BtsI GCAGTG_nn'
Cuts at: 664 1138 1409 1961 2506 2538 3746 664
Size: 474 271 552 545 32 1208 3421
Fragments arranged by size:
3421 1208 552 545 474 271 32
DraI TTT'AAA
Cuts at: 3502 3502
Size: 6503
DraIII CAC_nnn'GTG
Cuts at: 172 172
Size: 6503
DrdI GACnn_nn'nnGTC
Cuts at: 418 3115 4209 5067 418
Size: 2697 1094 858 1854
Fragments arranged by size:
2697 1854 1094 858
EaeI y'GGCC_r
Cuts at: 699 955 1253 1333 1425 1544 1694 1909 2087
Size: 256 298 80 92 119 150 215 178
Cuts at: 2087 2177 2598 4088 4262 4653 4680 6025 699
Size: 90 421 1490 174 391 27 1345 1177
Fragments arranged by size:
1490 1345 1177 421 391 298 256 215
178 174 150 119 92 90 80 27
EagI C'GGCC_G
Cuts at: 1333 1544 2598 4088 1333
Size: 211 1054 1490 3748
Fragments arranged by size:
3748 1490 1054 211
EarI CTCTTCn'nnn_
Cuts at: 281 368 1519 1567 2332 4526 4736 281
Size: 87 1151 48 765 2194 210 2048
Fragments arranged by size:
2194 2048 1151 765 210 87 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1681 4711 5021 5167 6313 1681
Size: 3030 310 146 1146 1871
Fragments arranged by size:
3030 1871 1146 310 146
EcoICRI GAG'CTC
Cuts at: 297 1859 2304 2592 2958 3515 297
Size: 1562 445 288 366 557 3285
Fragments arranged by size:
3285 1562 557 445 366 288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 299 460 592 729 733 843 932 1100
Size: 67 161 132 137 4 110 89 168
Cuts at: 1100 1237 1261 2002 2380 2506 2548 2815 2902
Size: 137 24 741 378 126 42 267 87
Cuts at: 2902 3007 3169 4328 4760 5507 5775 232
Size: 105 162 1159 432 747 268 960
Fragments arranged by size:
1159 960 747 741 432 378 268 267
168 162 161 137 137 132 126 110
105 89 87 67 42 24 4
EcoNI CCTnn'n_nnAGG
Cuts at: 451 1189 1300 1780 2134 3008 451
Size: 738 111 480 354 874 3946
Fragments arranged by size:
3946 874 738 480 354 111
EcoO109I rG'GnC_Cy
Cuts at: 137 190 191 444 781 1974 2072 2073 2165
Size: 53 1 253 337 1193 98 1 92
Cuts at: 2165 2261 2262 2342 2973 3001 3013 3219 3371
Size: 96 1 80 631 28 12 206 152
Cuts at: 3371 3642 137
Size: 271 2998
Fragments arranged by size:
2998 1193 631 337 271 253 206 152
98 96 92 80 53 28 12 1
1 1
EcoRI G'AATT_C
Cuts at: 3507 3507
Size: 6503
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4522 4522
Size: 6503
FseI GG_CCGG'CC
Cuts at: 1700 1700
Size: 6503
FspI TGC'GCA
Cuts at: 4284 4284
Size: 6503
HaeII r_GCGC'y
Cuts at: 2791 3937 4185 5207 6186 2791
Size: 1146 248 1022 979 3108
Fragments arranged by size:
3108 1146 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 600 632 911 943 2015 2047 3307 3339 4345
Size: 32 279 32 1072 32 1260 32 1006
Cuts at: 4345 4375 4377 4407 600
Size: 30 2 30 2696
Fragments arranged by size:
2696 1260 1072 1006 279 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3542 3542
Size: 6503
HindIII A'AGCT_T
Cuts at: 1096 2905 3255 1096
Size: 1809 350 4344
Fragments arranged by size:
4344 1809 350
KasI G'GCGC_C
Cuts at: 3933 4181 3933
Size: 248 6255
KpnI G_GTAC'C
Cuts at: 2484 3523 2484
Size: 1039 5464
MluI A'CGCG_T
Cuts at: 5936 5936
Size: 6503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1115 2217 5174 5358 1115
Size: 1102 2957 184 2260
Fragments arranged by size:
2957 2260 1102 184
MscI TGG'CCA
Cuts at: 1255 2089 2179 4264 1255
Size: 834 90 2085 3494
Fragments arranged by size:
3494 2085 834 90
MslI CAynn'nnrTG
Cuts at: 257 604 1186 4492 4619 257
Size: 347 582 3306 127 2141
Fragments arranged by size:
3306 2141 582 347 127
MspA1I CmG'CkG
Cuts at: 438 589 1078 1281 2205 2411 2523 2747 2950
Size: 151 489 203 924 206 112 224 203
Cuts at: 2950 3147 3614 3928 4288 5301 5546 438
Size: 197 467 314 360 1013 245 1395
Fragments arranged by size:
1395 1013 924 489 467 360 314 245
224 206 203 203 197 151 112
NaeI GCC'GGC
Cuts at: 1429 1698 4684 1429
Size: 269 2986 3248
Fragments arranged by size:
3248 2986 269
NarI GG'CG_CC
Cuts at: 3934 4182 3934
Size: 248 6255
NcoI C'CATG_G
Cuts at: 179 819 876 1817 179
Size: 640 57 941 4865
Fragments arranged by size:
4865 941 640 57
NgoMIV G'CCGG_C
Cuts at: 1427 1696 4682 1427
Size: 269 2986 3248
Fragments arranged by size:
3248 2986 269
NotI GC'GGCC_GC
Cuts at: 1333 1333
Size: 6503
NspI r_CATG'y
Cuts at: 1008 2647 3556 4056 4963 1008
Size: 1639 909 500 907 2548
Fragments arranged by size:
2548 1639 909 907 500
PasI CC'CwG_GG
Cuts at: 1521 1521
Size: 6503
PciI A'CATG_T
Cuts at: 2643 4959 2643
Size: 2316 4187
PfoI T'CCnGG_A
Cuts at: 425 1901 3455 425
Size: 1476 1554 3473
Fragments arranged by size:
3473 1554 1476
PmeI GTTT'AAAC
Cuts at: 3502 3502
Size: 6503
PmlI CAC'GTG
Cuts at: 1649 2580 1649
Size: 931 5572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2128 2160 2833 2865 5642 5674 2128
Size: 32 673 32 2777 32 2957
Fragments arranged by size:
2957 2777 673 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 137 781 2165 2973 137
Size: 644 1384 808 3667
Fragments arranged by size:
3667 1384 808 644
PspOMI G'GGCC_C
Cuts at: 190 1524 2072 2261 3000 3371 190
Size: 1334 548 189 739 371 3322
Fragments arranged by size:
3322 1334 739 548 371 189
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3340 3372 3340
Size: 32 6471
PstI C_TGCA'G
Cuts at: 1145 1505 2498 3068 3550 4235 1145
Size: 360 993 570 482 685 3413
Fragments arranged by size:
3413 993 685 570 482 360
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6503
PvuII CAG'CTG
Cuts at: 438 589 1078 1281 2523 2747 2950 3147 3928
Size: 151 489 203 1242 224 203 197 781
Cuts at: 3928 4288 438
Size: 360 2653
Fragments arranged by size:
2653 1242 781 489 360 224 203 203
197 151
RsrII CG'GwC_CG
Cuts at: 1654 4698 1654
Size: 3044 3459
SacI G_AGCT'C
Cuts at: 299 1861 2306 2594 2960 3517 299
Size: 1562 445 288 366 557 3285
Fragments arranged by size:
3285 1562 557 445 366 288
SacII CC_GC'GG
Cuts at: 2206 2206
Size: 6503
SalI G'TCGA_C
Cuts at: 3540 3540
Size: 6503
SanDI GG'GwC_CC
Cuts at: 781 781
Size: 6503
SapI GCTCTTCn'nnn_
Cuts at: 1519 4526 4736 1519
Size: 3007 210 3286
Fragments arranged by size:
3286 3007 210
SbfI CC_TGCA'GG
Cuts at: 1505 3550 1505
Size: 2045 4458
ScaI AGT'ACT
Cuts at: 6020 6020
Size: 6503
SfcI C'TryA_G
Cuts at: 33 1141 1501 2131 2494 3064 3546 4231 5224
Size: 1108 360 630 363 570 482 685 993
Cuts at: 5224 5415 33
Size: 191 1121
Fragments arranged by size:
1121 1108 993 685 630 570 482 363
360 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2042 2095 2042
Size: 53 6450
SfoI GGC'GCC
Cuts at: 3935 4183 3935
Size: 248 6255
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6503
SgrAI Cr'CCGG_yG
Cuts at: 1328 1662 1328
Size: 334 6169
SmaI CCC'GGG
Cuts at: 1756 2070 3525 1756
Size: 314 1455 4734
Fragments arranged by size:
4734 1455 314
SmlI C'TyrA_G
Cuts at: 2772 3658 4573 5065 5327 2772
Size: 886 915 492 262 3948
Fragments arranged by size:
3948 915 886 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6503
SphI G_CATG'C
Cuts at: 1008 3556 4056 1008
Size: 2548 500 3455
Fragments arranged by size:
3455 2548 500
StuI AGG'CCT
Cuts at: 1295 2659 1295
Size: 1364 5139
StyI C'CwwG_G
Cuts at: 156 179 819 876 1287 1319 1817 2329 2660
Size: 23 640 57 411 32 498 512 331
Cuts at: 2660 3155 3637 3991 156
Size: 495 482 354 2668
Fragments arranged by size:
2668 640 512 498 495 482 411 354
331 57 32 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1078 2213 3187 4086 4219 4678 4887 1078
Size: 1135 974 899 133 459 209 2694
Fragments arranged by size:
2694 1135 974 899 459 209 133
TatI w'GTAC_w
Cuts at: 2646 3260 3347 6018 2646
Size: 614 87 2671 3131
Fragments arranged by size:
3131 2671 614 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1039 1071 2894 2926 6020 6052 1039
Size: 32 1823 32 3094 32 1490
Fragments arranged by size:
3094 1823 1490 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1447 4300 1447
Size: 2853 3650
XbaI T'CTAG_A
Cuts at: 3534 3534
Size: 6503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 183 3998 114
Size: 69 3815 2619
Fragments arranged by size:
3815 2619 69
XmaI C'CCGG_G
Cuts at: 1754 2068 3523 1754
Size: 314 1455 4734
Fragments arranged by size:
4734 1455 314
ZraI GAC'GTC
Cuts at: 1055 3342 1055
Size: 2287 4216
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AscI AvrII BaeI BfrBI BmtI BsiWI
BsmI BssHII BstEII BstZ17I ClaI CspCI EcoRV FspAI
HpaI MfeI NdeI NheI NruI NsiI PacI PflMI
PshAI PsiI PspXI SexAI SnaBI SrfI SspI SwaI
XhoI XmnI