Restriction map for
pF1KE3386
(Circular) (Six-base) MAPSORT of: pF1KE3386.seq Check: 4527 from: 1 to: 6734
pF1KE3386 6734 bp
With 182 enzymes: SgfI *
June 25, 2015 11:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2467 2467
Size: 6734
AccI GT'mk_AC
Cuts at: 3772 3772
Size: 6734
Acc65I G'GTAC_C
Cuts at: 3750 3750
Size: 6734
AclI AA'CG_TT
Cuts at: 1869 6574 1869
Size: 4705 2029
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 617 1400 1526 1726 2305 3178 4559 4991
Size: 402 783 126 200 579 873 1381 432
Cuts at: 4991 5738 6006 215
Size: 747 268 943
Fragments arranged by size:
1381 943 873 783 747 579 432 402
268 200 126
AflIII A'CryG_T
Cuts at: 1411 2443 2668 4086 5190 6167 1411
Size: 1032 225 1418 1104 977 1978
Fragments arranged by size:
1978 1418 1104 1032 977 225
AgeI A'CCGG_T
Cuts at: 5122 5122
Size: 6734
AhdI GACnn_n'nnGTC
Cuts at: 1110 2523 1110
Size: 1413 5321
AleI CACnn'nnGTG
Cuts at: 1119 2873 1119
Size: 1754 4980
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1025 2355 1025
Size: 1330 5404
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1904 1936 1904
Size: 32 6702
AlwNI CAG_nnn'CTG
Cuts at: 3291 3451 4039 5606 3291
Size: 160 588 1567 4419
Fragments arranged by size:
4419 1567 588 160
ApaI G_GGCC'C
Cuts at: 3270 3696 3270
Size: 426 6308
ApaLI G'TGCA_C
Cuts at: 2866 5504 2866
Size: 2638 4096
ApoI r'AATT_y
Cuts at: 148 219 3738 6087 6343 148
Size: 71 3519 2349 256 539
Fragments arranged by size:
3519 2349 539 256 71
AseI AT'TA_AT
Cuts at: 20 1964 20
Size: 1944 4790
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6734
AvaI C'yCGr_G
Cuts at: 3754 3754
Size: 6734
BamHI G'GATC_C
Cuts at: 1234 3759 1234
Size: 2525 4209
BanI G'GyrC_C
Cuts at: 3750 4164 4412 4447 3750
Size: 414 248 35 6037
Fragments arranged by size:
6037 414 248 35
BanII G_rGCy'C
Cuts at: 3270 3512 3574 3696 3748 4778 3270
Size: 242 62 122 52 1030 5226
Fragments arranged by size:
5226 1030 242 122 62 52
BbeI G_GCGC'C
Cuts at: 4168 4416 4168
Size: 248 6486
BbsI GAAGACnn'nnnn_
Cuts at: 623 913 2145 623
Size: 290 1232 5212
Fragments arranged by size:
5212 1232 290
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6700
BciVI GTATCCnnnnn_n'
Cuts at: 1132 1488 3567 4632 5393 6734 1132
Size: 356 2079 1065 761 1341 1132
Fragments arranged by size:
2079 1341 1132 1065 761 356
BclI T'GATC_A
Cuts at: 338 4254 338
Size: 3916 2818
BfrBI ATG'CAT
Cuts at: 1596 3106 1596
Size: 1510 5224
BglI GCCn_nnn'nGGC
Cuts at: 3182 3182
Size: 6734
BglII A'GATC_T
Cuts at: 663 4249 6286 663
Size: 3586 2037 1111
Fragments arranged by size:
3586 2037 1111
BlpI GC'TnA_GC
Cuts at: 1627 3846 1627
Size: 2219 4515
Bme1580I G_kGCm'C
Cuts at: 1255 2752 2870 3270 3532 3696 4359 4452 5508
Size: 1497 118 400 262 164 663 93 1056
Cuts at: 5508 1255
Size: 2481
Fragments arranged by size:
2481 1497 1056 663 400 262 164 118
93
BmrI ACTGGGnnnn_n'
Cuts at: 1468 2033 2737 2869 3507 3946 4027 4362 6506
Size: 565 704 132 638 439 81 335 2144
Cuts at: 6506 1468
Size: 1696
Fragments arranged by size:
2144 1696 704 638 565 439 335 132
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 682 1914 2284 5101 682
Size: 1232 370 2817 2315
Fragments arranged by size:
2817 2315 1232 370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 1093 204
Size: 889 5845
Bpu10I CC'TnA_GC
Cuts at: 1046 3253 1046
Size: 2207 4527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1414 1562 3412 3910 4789 5281 5579 1414
Size: 148 1850 498 879 492 298 2569
Fragments arranged by size:
2569 1850 879 498 492 298 148
BsaI GGTCTCn'nnnn_
Cuts at: 1148 3146 6438 1148
Size: 1998 3292 1444
Fragments arranged by size:
3292 1998 1444
BsaAI yAC'GTr
Cuts at: 1250 4087 1250
Size: 2837 3897
BsaBI GATnn'nnATC
Cuts at: 6 2462 4248 6285 6
Size: 2456 1786 2037 455
Fragments arranged by size:
2456 2037 1786 455
BsaHI Gr'CG_yC
Cuts at: 2934 4165 4413 5115 6611 2934
Size: 1231 248 702 1496 3057
Fragments arranged by size:
3057 1496 1231 702 248
BsaWI w'CCGG_w
Cuts at: 1157 3923 4444 5122 5396 5543 5954 1157
Size: 2766 521 678 274 147 411 1937
Fragments arranged by size:
2766 1937 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 803 833 1757 1787 3499 3529 803
Size: 30 924 30 1712 30 4008
Fragments arranged by size:
4008 1712 924 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2751 3238 3337 3357 2751
Size: 487 99 20 6128
Fragments arranged by size:
6128 487 99 20
BseYI C'CCAG_C
Cuts at: 1739 2692 4159 4917 5494 5990 1739
Size: 953 1467 758 577 496 2483
Fragments arranged by size:
2483 1467 953 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 1151 1230 1556 2877 224
Size: 927 79 326 1321 4081
Fragments arranged by size:
4081 1321 927 326 79
BsiEI CG_ry'CG
Cuts at: 104 4276 4322 5530 104
Size: 4172 46 1208 1308
Fragments arranged by size:
4172 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2616 2806 2870 3748 4526 4716 5508 2616
Size: 190 64 878 778 190 792 3842
Fragments arranged by size:
3842 878 792 778 190 190 64
BsmI GAATG_Cn'
Cuts at: 1333 2889 1333
Size: 1556 5178
BsmBI CGTCTCn'nnnn_
Cuts at: 2272 2272
Size: 6734
Bsp1286I G_dGCh'C
Cuts at: 1255 2616 2752 2806 2870 3270 3512 3532 3574
Size: 1361 136 54 64 400 242 20 42
Cuts at: 3574 3696 3748 4359 4452 4526 4716 4778 5508
Size: 122 52 611 93 74 190 62 730
Cuts at: 5508 1255
Size: 2481
Fragments arranged by size:
2481 1361 730 611 400 242 190 136
122 93 74 64 62 54 52 42
20
BspEI T'CCGG_A
Cuts at: 5954 5954
Size: 6734
BspHI T'CATG_A
Cuts at: 1662 5910 1662
Size: 4248 2486
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2467 2926 3784 3998 4300 4681 2467
Size: 459 858 214 302 381 4520
Fragments arranged by size:
4520 858 459 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5026 5080 6564 6729 50
Size: 4976 54 1484 165 55
Fragments arranged by size:
4976 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 866 1323 4646 866
Size: 457 3323 2954
Fragments arranged by size:
3323 2954 457
BsrFI r'CCGG_y
Cuts at: 2798 4732 4913 5122 2798
Size: 1934 181 209 4410
Fragments arranged by size:
4410 1934 209 181
BssSI C'ACGA_G
Cuts at: 1907 5005 5363 1907
Size: 3098 358 3278
Fragments arranged by size:
3278 3098 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4111 4111
Size: 6734
BstBI TT'CG_AA
Cuts at: 5095 5095
Size: 6734
BstEII G'GTnAC_C
Cuts at: 874 2161 874
Size: 1287 5447
BstXI CCAn_nnnn'nTGG
Cuts at: 1481 1999 2824 3497 3520 1481
Size: 518 825 673 23 4695
Fragments arranged by size:
4695 825 673 518 23
BstYI r'GATC_y
Cuts at: 1 663 1234 2334 3759 4226 4249 4584 4830
Size: 662 571 1100 1425 467 23 335 246
Cuts at: 4830 5842 5928 5940 6286 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1425 1100 1012 662 571 467 449 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2986 2986
Size: 6734
BtgI C'CryG_G
Cuts at: 261 3186 261
Size: 2925 3809
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3153 4684 4865 5028 3153
Size: 1531 181 163 4859
Fragments arranged by size:
4859 1531 181 163
BtsI GCAGTG_nn'
Cuts at: 2186 3977 2186
Size: 1791 4943
DraI TTT'AAA
Cuts at: 578 1859 3733 578
Size: 1281 1874 3579
Fragments arranged by size:
3579 1874 1281
DraIII CAC_nnn'GTG
Cuts at: 985 1094 985
Size: 109 6625
DrdI GACnn_nn'nnGTC
Cuts at: 2538 4440 5298 2538
Size: 1902 858 3974
Fragments arranged by size:
3974 1902 858
EaeI y'GGCC_r
Cuts at: 1803 1931 2001 2111 3183 3462 4319 4493 4884
Size: 128 70 110 1072 279 857 174 391
Cuts at: 4884 4911 6256 1803
Size: 27 1345 2281
Fragments arranged by size:
2281 1345 1072 857 391 279 174 128
110 70 27
EagI C'GGCC_G
Cuts at: 4319 4319
Size: 6734
EarI CTCTTCn'nnn_
Cuts at: 4757 4967 4757
Size: 210 6524
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4942 5252 5398 6544 4942
Size: 310 146 1146 5132
Fragments arranged by size:
5132 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3746 3746
Size: 6734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 215 617 1093 1400 1526 1726 2305 3178
Size: 11 402 476 307 126 200 579 873
Cuts at: 3178 4559 4991 5738 6006 204
Size: 1381 432 747 268 932
Fragments arranged by size:
1381 932 873 747 579 476 432 402
307 268 200 126 11
EcoO109I rG'GnC_Cy
Cuts at: 235 1360 3266 3692 3693 3873 235
Size: 1125 1906 426 1 180 3096
Fragments arranged by size:
3096 1906 1125 426 180 1
EcoRI G'AATT_C
Cuts at: 219 3738 219
Size: 3519 3215
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1704 4753 1704
Size: 3049 3685
FspI TGC'GCA
Cuts at: 4515 4515
Size: 6734
HaeII r_GCGC'y
Cuts at: 4168 4416 5438 6417 4168
Size: 248 1022 979 4485
Fragments arranged by size:
4485 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 803 835 1096 1128 2509 2541 2820 2852 3361
Size: 32 261 32 1381 32 279 32 509
Cuts at: 3361 3393 3556 3588 4576 4606 4608 4638 803
Size: 32 163 32 988 30 2 30 2899
Fragments arranged by size:
2899 1381 988 509 279 261 163 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3442 3773 3442
Size: 331 6403
HindIII A'AGCT_T
Cuts at: 3597 3597
Size: 6734
KasI G'GCGC_C
Cuts at: 4164 4412 4164
Size: 248 6486
KpnI G_GTAC'C
Cuts at: 3754 3754
Size: 6734
MfeI C'AATT_G
Cuts at: 310 310
Size: 6734
MluI A'CGCG_T
Cuts at: 6167 6167
Size: 6734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 674 862 5405 5589 674
Size: 188 4543 184 1819
Fragments arranged by size:
4543 1819 188 184
MscI TGG'CCA
Cuts at: 1933 2113 3185 3464 4495 1933
Size: 180 1072 279 1031 4172
Fragments arranged by size:
4172 1072 1031 279 180
MslI CAynn'nnrTG
Cuts at: 356 1119 1340 1601 2575 2673 2873 3068 3211
Size: 763 221 261 974 98 200 195 143
Cuts at: 3211 4723 4850 356
Size: 1512 127 2240
Fragments arranged by size:
2240 1512 974 763 261 221 200 195
143 127 98
MspA1I CmG'CkG
Cuts at: 718 2469 2873 2891 3845 4159 4519 5532 5777
Size: 1751 404 18 954 314 360 1013 245
Cuts at: 5777 718
Size: 1675
Fragments arranged by size:
1751 1675 1013 954 404 360 314 245
18
NaeI GCC'GGC
Cuts at: 4915 4915
Size: 6734
NarI GG'CG_CC
Cuts at: 4165 4413 4165
Size: 248 6486
NcoI C'CATG_G
Cuts at: 261 261
Size: 6734
NdeI CA'TA_TG
Cuts at: 3605 3605
Size: 6734
NgoMIV G'CCGG_C
Cuts at: 4913 4913
Size: 6734
NsiI A_TGCA'T
Cuts at: 1598 3108 1598
Size: 1510 5224
NspI r_CATG'y
Cuts at: 1128 1415 1596 2283 2574 2634 2672 2925 3081
Size: 287 181 687 291 60 38 253 156
Cuts at: 3081 3110 3787 4287 5194 1128
Size: 29 677 500 907 2668
Fragments arranged by size:
2668 907 687 677 500 291 287 253
181 156 60 38 29
PciI A'CATG_T
Cuts at: 1411 2668 5190 1411
Size: 1257 2522 2955
Fragments arranged by size:
2955 2522 1257
PflMI CCAn_nnn'nTGG
Cuts at: 1638 3451 3588 1638
Size: 1813 137 4784
Fragments arranged by size:
4784 1813 137
PmeI GTTT'AAAC
Cuts at: 3733 3733
Size: 6734
PmlI CAC'GTG
Cuts at: 1250 1250
Size: 6734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5873 5905 5873
Size: 32 6702
PpuMI rG'GwC_Cy
Cuts at: 235 1360 235
Size: 1125 5609
PshAI GACnn'nnGTC
Cuts at: 2937 2937
Size: 6734
PsiI TTA'TAA
Cuts at: 356 3409 356
Size: 3053 3681
PspOMI G'GGCC_C
Cuts at: 3266 3692 3266
Size: 426 6308
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1250 1282 1407 1439 1250
Size: 32 125 32 6545
Fragments arranged by size:
6545 125 32 32
PstI C_TGCA'G
Cuts at: 1084 3781 4466 1084
Size: 2697 685 3352
Fragments arranged by size:
3352 2697 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6734
PvuII CAG'CTG
Cuts at: 718 2469 2873 2891 4159 4519 718
Size: 1751 404 18 1268 360 2933
Fragments arranged by size:
2933 1751 1268 404 360 18
RsrII CG'GwC_CG
Cuts at: 4929 4929
Size: 6734
SacI G_AGCT'C
Cuts at: 3748 3748
Size: 6734
SalI G'TCGA_C
Cuts at: 3771 3771
Size: 6734
SapI GCTCTTCn'nnn_
Cuts at: 4757 4967 4757
Size: 210 6524
SbfI CC_TGCA'GG
Cuts at: 3781 3781
Size: 6734
ScaI AGT'ACT
Cuts at: 1165 6251 1165
Size: 5086 1648
SfcI C'TryA_G
Cuts at: 33 1080 1256 2174 3276 3777 4462 5455 5646
Size: 1047 176 918 1102 501 685 993 191
Cuts at: 5646 33
Size: 1121
Fragments arranged by size:
1121 1102 1047 993 918 685 501 191
176
SfoI GGC'GCC
Cuts at: 4166 4414 4166
Size: 248 6486
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6734
SmaI CCC'GGG
Cuts at: 3756 3756
Size: 6734
SmlI C'TyrA_G
Cuts at: 1393 1577 3427 3889 4804 5296 5558 1393
Size: 184 1850 462 915 492 262 2569
Fragments arranged by size:
2569 1850 915 492 462 262 184
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6734
SphI G_CATG'C
Cuts at: 2283 3110 3787 4287 2283
Size: 827 677 500 4730
Fragments arranged by size:
4730 827 677 500
StuI AGG'CCT
Cuts at: 1997 3354 1997
Size: 1357 5377
StyI C'CwwG_G
Cuts at: 261 1019 1992 1998 2271 2817 3394 3868 4222
Size: 758 973 6 273 546 577 474 354
Cuts at: 4222 261
Size: 2773
Fragments arranged by size:
2773 973 758 577 546 474 354 273
6
SwaI ATTT'AAAT
Cuts at: 1859 1859
Size: 6734
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 366 1205 2180 2725 4317 4450 4909 5118 366
Size: 839 975 545 1592 133 459 209 1982
Fragments arranged by size:
1982 1592 975 839 545 459 209 133
TatI w'GTAC_w
Cuts at: 761 1163 1207 2075 6249 761
Size: 402 44 868 4174 1246
Fragments arranged by size:
4174 1246 868 402 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1152 1184 1757 1789 2182 2214 2992 3024 6251
Size: 32 573 32 393 32 778 32 3227
Cuts at: 6251 6283 1152
Size: 32 1603
Fragments arranged by size:
3227 1603 778 573 393 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4531 4531
Size: 6734
XbaI T'CTAG_A
Cuts at: 3765 3765
Size: 6734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3472 4229 3472
Size: 757 5977
XmaI C'CCGG_G
Cuts at: 3754 3754
Size: 6734
XmnI GAAnn'nnTTC
Cuts at: 552 780 552
Size: 228 6506
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AscI AvrII BaeI BbvCI BmgBI
BmtI BsiWI BsrGI BssHII BstZ17I ClaI CspCI EcoNI
EcoRV FseI FspAI HpaI NheI NotI NruI PacI
PasI PfoI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI XhoI ZraI