Restriction map for
pF1KE3388
(Circular) (Six-base) MAPSORT of: pF1KE3388.seq Check: 3929 from: 1 to: 6755
pF1KE3388 6755 bp
With 182 enzymes: SgfI *
October 12, 2011 16:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2841 2841
Size: 6755
AccI GT'mk_AC
Cuts at: 3793 3793
Size: 6755
Acc65I G'GTAC_C
Cuts at: 2671 3771 2671
Size: 1100 5655
AclI AA'CG_TT
Cuts at: 6595 6595
Size: 6755
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1494 1850 1975 2596 2974 3225 4580 5012 5759
Size: 356 125 621 378 251 1355 432 747
Cuts at: 5759 6027 1494
Size: 268 2222
Fragments arranged by size:
2222 1355 747 621 432 378 356 268
251 125
AfeI AGC'GCT
Cuts at: 718 718
Size: 6755
AflIII A'CryG_T
Cuts at: 4107 5211 6188 4107
Size: 1104 977 4674
Fragments arranged by size:
4674 1104 977
AgeI A'CCGG_T
Cuts at: 5143 5143
Size: 6755
AhdI GACnn_n'nnGTC
Cuts at: 1773 1773
Size: 6755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 901 1447 1908 901
Size: 546 461 5748
Fragments arranged by size:
5748 546 461
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3515 3547 3515
Size: 32 6723
AlwNI CAG_nnn'CTG
Cuts at: 1856 2922 3132 3584 4060 5627 1856
Size: 1066 210 452 476 1567 2984
Fragments arranged by size:
2984 1567 1066 476 452 210
ApaLI G'TGCA_C
Cuts at: 852 5525 852
Size: 4673 2082
ApoI r'AATT_y
Cuts at: 700 3454 3759 6108 6364 700
Size: 2754 305 2349 256 1091
Fragments arranged by size:
2754 2349 1091 305 256
AseI AT'TA_AT
Cuts at: 20 2336 20
Size: 2316 4439
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6755
AvaI C'yCGr_G
Cuts at: 169 193 1346 1922 2765 3775 169
Size: 24 1153 576 843 1010 3149
Fragments arranged by size:
3149 1153 1010 843 576 24
AvrII C'CTAG_G
Cuts at: 2193 2193
Size: 6755
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 597 630 597
Size: 33 6722
BamHI G'GATC_C
Cuts at: 3300 3698 3780 3300
Size: 398 82 6275
Fragments arranged by size:
6275 398 82
BanI G'GyrC_C
Cuts at: 124 2671 3771 4185 4433 4468 124
Size: 2547 1100 414 248 35 2411
Fragments arranged by size:
2547 2411 1100 414 248 35
BanII G_rGCy'C
Cuts at: 194 206 563 1366 1693 3206 3249 3401 3528
Size: 12 357 803 327 1513 43 152 127
Cuts at: 3528 3628 3635 3769 4799 194
Size: 100 7 134 1030 2150
Fragments arranged by size:
2150 1513 1030 803 357 327 152 134
127 100 43 12 7
BbeI G_GCGC'C
Cuts at: 128 4189 4437 128
Size: 4061 248 2446
Fragments arranged by size:
4061 2446 248
BbsI GAAGACnn'nnnn_
Cuts at: 268 1071 1152 1511 1556 1684 2602 2972 268
Size: 803 81 359 45 128 918 370 4051
Fragments arranged by size:
4051 918 803 370 359 128 81 45
BciVI GTATCCnnnnn_n'
Cuts at: 3100 4653 5414 6755 3100
Size: 1553 761 1341 3100
Fragments arranged by size:
3100 1553 1341 761
BclI T'GATC_A
Cuts at: 2171 4275 2171
Size: 2104 4651
BglI GCCn_nnn'nGGC
Cuts at: 112 367 971 1825 2237 3745 112
Size: 255 604 854 412 1508 3122
Fragments arranged by size:
3122 1508 854 604 412 255
BglII A'GATC_T
Cuts at: 1397 2816 4270 6307 1397
Size: 1419 1454 2037 1845
Fragments arranged by size:
2037 1845 1454 1419
BlpI GC'TnA_GC
Cuts at: 1365 3721 3867 1365
Size: 2356 146 4253
Fragments arranged by size:
4253 2356 146
Bme1580I G_kGCm'C
Cuts at: 856 967 2089 2916 4380 4473 5529 856
Size: 111 1122 827 1464 93 1056 2082
Fragments arranged by size:
2082 1464 1122 1056 827 111 93
BmgBI CAC'GTC
Cuts at: 242 671 242
Size: 429 6326
BmrI ACTGGGnnnn_n'
Cuts at: 561 1363 2600 2793 3967 4048 4383 6527 561
Size: 802 1237 193 1174 81 335 2144 789
Fragments arranged by size:
2144 1237 1174 802 789 335 193 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 225 1948 5122 225
Size: 1723 3174 1858
Fragments arranged by size:
3174 1858 1723
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2578 2596 2743 3589 3664 2578
Size: 18 147 846 75 5669
Fragments arranged by size:
5669 846 147 75 18
Bpu10I CC'TnA_GC
Cuts at: 3355 3355
Size: 6755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1741 3643 3931 4810 5302 5600 1741
Size: 1902 288 879 492 298 2896
Fragments arranged by size:
2896 1902 879 492 298 288
BsaI GGTCTCn'nnnn_
Cuts at: 1865 6459 1865
Size: 4594 2161
BsaAI yAC'GTr
Cuts at: 4108 4108
Size: 6755
BsaBI GATnn'nnATC
Cuts at: 6 2821 4269 6306 6
Size: 2815 1448 2037 455
Fragments arranged by size:
2815 2037 1448 455
BsaHI Gr'CG_yC
Cuts at: 125 4186 4434 5136 6632 125
Size: 4061 248 702 1496 248
Fragments arranged by size:
4061 1496 702 248 248
BsaWI w'CCGG_w
Cuts at: 1223 1867 3944 4465 5143 5417 5564 5975 1223
Size: 644 2077 521 678 274 147 411 2003
Fragments arranged by size:
2077 2003 678 644 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3010 3040 3515 3545 3010
Size: 30 475 30 6220
Fragments arranged by size:
6220 475 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 187 1476 1479 1602 1711 1837 2077 2145 3046
Size: 1289 3 123 109 126 240 68 901
Cuts at: 3046 3049 3565 187
Size: 3 516 3377
Fragments arranged by size:
3377 1289 901 516 240 126 123 109
68 3 3
BseYI C'CCAG_C
Cuts at: 1259 1308 1358 1385 2219 2638 3321 4180 4938
Size: 49 50 27 834 419 683 859 758
Cuts at: 4938 5515 6011 1259
Size: 577 496 2003
Fragments arranged by size:
2003 859 834 758 683 577 496 419
50 49 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 2499 2521 2869 2995 3622 559
Size: 1940 22 348 126 627 3692
Fragments arranged by size:
3692 1940 627 348 126 22
BsiEI CG_ry'CG
Cuts at: 104 770 1685 1906 4297 4343 5551 104
Size: 666 915 221 2391 46 1208 1308
Fragments arranged by size:
2391 1308 1208 915 666 221 46
BsiHKAI G_wGCw'C
Cuts at: 554 856 1628 1693 1726 2468 2950 3249 3333
Size: 302 772 65 33 742 482 299 84
Cuts at: 3333 3575 3635 3769 4547 4737 5529 554
Size: 242 60 134 778 190 792 1780
Fragments arranged by size:
1780 792 778 772 742 482 302 299
242 190 134 84 65 60 33
BsmI GAATG_Cn'
Cuts at: 2016 2016
Size: 6755
BsmBI CGTCTCn'nnnn_
Cuts at: 1627 2766 3253 1627
Size: 1139 487 5129
Fragments arranged by size:
5129 1139 487
Bsp1286I G_dGCh'C
Cuts at: 194 206 554 563 856 967 1366 1628 1693
Size: 12 348 9 293 111 399 262 65
Cuts at: 1693 1726 2089 2468 2916 2950 3206 3249 3333
Size: 33 363 379 448 34 256 43 84
Cuts at: 3333 3401 3528 3575 3628 3635 3769 4380 4473
Size: 68 127 47 53 7 134 611 93
Cuts at: 4473 4547 4737 4799 5529 194
Size: 74 190 62 730 1420
Fragments arranged by size:
1420 730 611 448 399 379 363 348
293 262 256 190 134 127 111 93
84 74 68 65 62 53 47 43
34 33 12 9 7
BspEI T'CCGG_A
Cuts at: 1223 5975 1223
Size: 4752 2003
BspHI T'CATG_A
Cuts at: 1169 2396 5931 1169
Size: 1227 3535 1993
Fragments arranged by size:
3535 1993 1227
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2841 3805 4019 4321 4702 2841
Size: 964 214 302 381 4894
Fragments arranged by size:
4894 964 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1323 3648 5047 5101 6585 6750 50
Size: 1273 2325 1399 54 1484 165 55
Fragments arranged by size:
2325 1484 1399 1273 165 55 54
BsrDI GCAATG_nn'
Cuts at: 645 812 1814 4667 645
Size: 167 1002 2853 2733
Fragments arranged by size:
2853 2733 1002 167
BsrFI r'CCGG_y
Cuts at: 4753 4934 5143 4753
Size: 181 209 6365
Fragments arranged by size:
6365 209 181
BssSI C'ACGA_G
Cuts at: 3463 5026 5384 3463
Size: 1563 358 4834
Fragments arranged by size:
4834 1563 358
BstAPI GCAn_nnn'nTGC
Cuts at: 644 860 1856 4132 644
Size: 216 996 2276 3267
Fragments arranged by size:
3267 2276 996 216
BstBI TT'CG_AA
Cuts at: 5116 5116
Size: 6755
BstXI CCAn_nnnn'nTGG
Cuts at: 1434 3048 3165 1434
Size: 1614 117 5024
Fragments arranged by size:
5024 1614 117
BstYI r'GATC_y
Cuts at: 1 251 396 1397 2261 2816 3300 3426 3698
Size: 250 145 1001 864 555 484 126 272
Cuts at: 3698 3780 4247 4270 4605 4851 5863 5949 5961
Size: 82 467 23 335 246 1012 86 12
Cuts at: 5961 6307 1
Size: 346 449
Fragments arranged by size:
1012 1001 864 555 484 467 449 346
335 272 250 246 145 126 86 82
23 12
BtgI C'CryG_G
Cuts at: 107 923 1001 3164 3376 107
Size: 816 78 2163 212 3486
Fragments arranged by size:
3486 2163 816 212 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1388 2474 4705 4886 5049 1388
Size: 1086 2231 181 163 3094
Fragments arranged by size:
3094 2231 1086 181 163
BtsI GCAGTG_nn'
Cuts at: 885 3261 3453 3998 885
Size: 2376 192 545 3642
Fragments arranged by size:
3642 2376 545 192
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 855 890 855
Size: 35 6720
DraI TTT'AAA
Cuts at: 3477 3754 3477
Size: 277 6478
DraIII CAC_nnn'GTG
Cuts at: 860 938 1602 2082 3377 860
Size: 78 664 480 1295 4238
Fragments arranged by size:
4238 1295 664 480 78
DrdI GACnn_nn'nnGTC
Cuts at: 4461 5319 4461
Size: 858 5897
EaeI y'GGCC_r
Cuts at: 1616 1712 1903 2756 4340 4514 4905 4932 6277
Size: 96 191 853 1584 174 391 27 1345
Cuts at: 6277 1616
Size: 2094
Fragments arranged by size:
2094 1584 1345 853 391 191 174 96
27
EagI C'GGCC_G
Cuts at: 1903 4340 1903
Size: 2437 4318
EarI CTCTTCn'nnn_
Cuts at: 1546 3404 3688 4778 4988 1546
Size: 1858 284 1090 210 3313
Fragments arranged by size:
3313 1858 1090 284 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 122 146 214 2204 3293 3448 4963 5273 5419
Size: 24 68 1990 1089 155 1515 310 146
Cuts at: 5419 6565 122
Size: 1146 312
Fragments arranged by size:
1990 1515 1146 1089 312 310 155 146
68 24
EcoICRI GAG'CTC
Cuts at: 1691 3247 3633 3767 1691
Size: 1556 386 134 4679
Fragments arranged by size:
4679 1556 386 134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1494 1850 1975 2578 2596 2743 2974 3225 3589
Size: 356 125 603 18 147 231 251 364
Cuts at: 3589 3664 4580 5012 5759 6027 1494
Size: 75 916 432 747 268 2222
Fragments arranged by size:
2222 916 747 603 432 364 356 268
251 231 147 125 75 18
EcoO109I rG'GnC_Cy
Cuts at: 139 1263 2118 3075 3654 3663 3894 139
Size: 1124 855 957 579 9 231 3000
Fragments arranged by size:
3000 1124 957 855 579 231 9
EcoRI G'AATT_C
Cuts at: 3759 3759
Size: 6755
EcoRV GAT'ATC
Cuts at: 2435 2435
Size: 6755
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 498 3618 4774 498
Size: 3120 1156 2479
Fragments arranged by size:
3120 2479 1156
FspI TGC'GCA
Cuts at: 3253 4536 3253
Size: 1283 5472
HaeII r_GCGC'y
Cuts at: 128 720 848 906 3734 4189 4437 5459 6438
Size: 592 128 58 2828 455 248 1022 979
Cuts at: 6438 128
Size: 445
Fragments arranged by size:
2828 1022 979 592 455 445 248 128
58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 571 603 643 675 1582 1614 1759
Size: 32 376 32 40 32 907 32 145
Cuts at: 1759 1791 3010 3042 3411 3443 4597 4627 4629
Size: 32 1219 32 369 32 1154 30 2
Cuts at: 4629 4659 163
Size: 30 2259
Fragments arranged by size:
2259 1219 1154 907 376 369 145 40
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 441 3794 441
Size: 3353 3402
HindIII A'AGCT_T
Cuts at: 334 334
Size: 6755
KasI G'GCGC_C
Cuts at: 124 4185 4433 124
Size: 4061 248 2446
Fragments arranged by size:
4061 2446 248
KpnI G_GTAC'C
Cuts at: 2675 3775 2675
Size: 1100 5655
MfeI C'AATT_G
Cuts at: 600 600
Size: 6755
MluI A'CGCG_T
Cuts at: 6188 6188
Size: 6755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 206 279 803 1391 5426 5610 206
Size: 73 524 588 4035 184 1351
Fragments arranged by size:
4035 1351 588 524 184 73
MscI TGG'CCA
Cuts at: 1714 4516 1714
Size: 2802 3953
MslI CAynn'nnrTG
Cuts at: 1429 2752 3046 4744 4871 1429
Size: 1323 294 1698 127 3313
Fragments arranged by size:
3313 1698 1323 294 127
MspA1I CmG'CkG
Cuts at: 1046 1308 3297 3321 3866 4180 4540 5553 5798
Size: 262 1989 24 545 314 360 1013 245
Cuts at: 5798 1046
Size: 2003
Fragments arranged by size:
2003 1989 1013 545 360 314 262 245
24
NaeI GCC'GGC
Cuts at: 4936 4936
Size: 6755
NarI GG'CG_CC
Cuts at: 125 4186 4434 125
Size: 4061 248 2446
Fragments arranged by size:
4061 2446 248
NcoI C'CATG_G
Cuts at: 107 923 3164 107
Size: 816 2241 3698
Fragments arranged by size:
3698 2241 816
NgoMIV G'CCGG_C
Cuts at: 4934 4934
Size: 6755
NspI r_CATG'y
Cuts at: 2751 3808 4308 5215 2751
Size: 1057 500 907 4291
Fragments arranged by size:
4291 1057 907 500
PacI TTA_AT'TAA
Cuts at: 2336 2336
Size: 6755
PciI A'CATG_T
Cuts at: 5211 5211
Size: 6755
PflMI CCAn_nnn'nTGG
Cuts at: 822 1401 2663 822
Size: 579 1262 4914
Fragments arranged by size:
4914 1262 579
PfoI T'CCnGG_A
Cuts at: 3221 3221
Size: 6755
PmeI GTTT'AAAC
Cuts at: 3754 3754
Size: 6755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3515 3547 5894 5926 3515
Size: 32 2347 32 4344
Fragments arranged by size:
4344 2347 32 32
PpuMI rG'GwC_Cy
Cuts at: 139 1263 2118 3075 3654 3663 139
Size: 1124 855 957 579 9 3231
Fragments arranged by size:
3231 1124 957 855 579 9
PstI C_TGCA'G
Cuts at: 896 3214 3296 3802 4487 896
Size: 2318 82 506 685 3164
Fragments arranged by size:
3164 2318 685 506 82
PvuI CG_AT'CG
Cuts at: 104 770 104
Size: 666 6089
PvuII CAG'CTG
Cuts at: 1046 1308 3297 3321 4180 4540 1046
Size: 262 1989 24 859 360 3261
Fragments arranged by size:
3261 1989 859 360 262 24
RsrII CG'GwC_CG
Cuts at: 4950 4950
Size: 6755
SacI G_AGCT'C
Cuts at: 1693 3249 3635 3769 1693
Size: 1556 386 134 4679
Fragments arranged by size:
4679 1556 386 134
SalI G'TCGA_C
Cuts at: 3792 3792
Size: 6755
SanDI GG'GwC_CC
Cuts at: 139 2118 139
Size: 1979 4776
SapI GCTCTTCn'nnn_
Cuts at: 4778 4988 4778
Size: 210 6545
SbfI CC_TGCA'GG
Cuts at: 896 3802 896
Size: 2906 3849
ScaI AGT'ACT
Cuts at: 1352 6272 1352
Size: 4920 1835
SfcI C'TryA_G
Cuts at: 33 892 2779 3210 3292 3798 4483 5476 5667
Size: 859 1887 431 82 506 685 993 191
Cuts at: 5667 33
Size: 1121
Fragments arranged by size:
1887 1121 993 859 685 506 431 191
82
SfoI GGC'GCC
Cuts at: 126 4187 4435 126
Size: 4061 248 2446
Fragments arranged by size:
4061 2446 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6755
SmaI CCC'GGG
Cuts at: 3777 3777
Size: 6755
SmlI C'TyrA_G
Cuts at: 1756 1922 2765 3658 3910 4825 5317 5579 1756
Size: 166 843 893 252 915 492 262 2932
Fragments arranged by size:
2932 915 893 843 492 262 252 166
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6755
SphI G_CATG'C
Cuts at: 2751 3808 4308 2751
Size: 1057 500 5198
Fragments arranged by size:
5198 1057 500
StuI AGG'CCT
Cuts at: 1483 1669 1783 2866 1483
Size: 186 114 1083 5372
Fragments arranged by size:
5372 1083 186 114
StyI C'CwwG_G
Cuts at: 107 923 1763 2123 2193 2675 2826 3164 3677
Size: 816 840 360 70 482 151 338 513
Cuts at: 3677 3889 4243 107
Size: 212 354 2619
Fragments arranged by size:
2619 840 816 513 482 360 354 338
212 151 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 580 1014 1399 1699 2483 3058 3362 4338 4471
Size: 434 385 300 784 575 304 976 133
Cuts at: 4471 4930 5139 580
Size: 459 209 2196
Fragments arranged by size:
2196 976 784 575 459 434 385 304
300 209 133
TatI w'GTAC_w
Cuts at: 569 1350 6270 569
Size: 781 4920 1054
Fragments arranged by size:
4920 1054 781
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 667 699 3690 3722 6272 6304 667
Size: 32 2991 32 2550 32 1118
Fragments arranged by size:
2991 2550 1118 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4552 4552
Size: 6755
XbaI T'CTAG_A
Cuts at: 3786 3786
Size: 6755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4250 4250
Size: 6755
XhoI C'TCGA_G
Cuts at: 1922 2765 1922
Size: 843 5912
XmaI C'CCGG_G
Cuts at: 3775 3775
Size: 6755
XmnI GAAnn'nnTTC
Cuts at: 1645 3238 1645
Size: 1593 5162
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PacI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI ApaI AscI BbvCI BcgI BfrBI
BmtI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
EcoNI FseI FspAI HpaI NdeI NheI NotI NruI
NsiI PasI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
ZraI