Restriction map for
pF1KE3393
(Circular) (Six-base) MAPSORT of: pF1KE3393.seq Check: 2208 from: 1 to: 6677
pF1KE3393 6677 bp
With 182 enzymes: SgfI *
September 21, 2017 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3093 3093
Size: 6677
AccI GT'mk_AC
Cuts at: 373 803 3715 373
Size: 430 2912 3335
Fragments arranged by size:
3335 2912 430
Acc65I G'GTAC_C
Cuts at: 3274 3693 3274
Size: 419 6258
AclI AA'CG_TT
Cuts at: 6517 6517
Size: 6677
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 835 980 1055 1131 1811 2401 2930 3227
Size: 254 145 75 76 680 590 529 297
Cuts at: 3227 3536 4502 4934 5681 5949 581
Size: 309 966 432 747 268 1309
Fragments arranged by size:
1309 966 747 680 590 529 432 309
297 268 254 145 76 75
AflIII A'CryG_T
Cuts at: 4029 5133 6110 4029
Size: 1104 977 4596
Fragments arranged by size:
4596 1104 977
AgeI A'CCGG_T
Cuts at: 5065 5065
Size: 6677
AlwNI CAG_nnn'CTG
Cuts at: 3613 3982 5549 3613
Size: 369 1567 4741
Fragments arranged by size:
4741 1567 369
ApaLI G'TGCA_C
Cuts at: 5447 5447
Size: 6677
ApoI r'AATT_y
Cuts at: 766 1208 1239 1539 1877 2232 2283 2802 3681
Size: 442 31 300 338 355 51 519 879
Cuts at: 3681 6030 6286 766
Size: 2349 256 1157
Fragments arranged by size:
2349 1157 879 519 442 355 338 300
256 51 31
AseI AT'TA_AT
Cuts at: 20 3352 20
Size: 3332 3345
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6677
AvaI C'yCGr_G
Cuts at: 1529 3697 1529
Size: 2168 4509
BamHI G'GATC_C
Cuts at: 3702 3702
Size: 6677
BanI G'GyrC_C
Cuts at: 172 1011 3274 3693 4107 4355 4390 172
Size: 839 2263 419 414 248 35 2459
Fragments arranged by size:
2459 2263 839 419 414 248 35
BanII G_rGCy'C
Cuts at: 833 3691 4721 833
Size: 2858 1030 2789
Fragments arranged by size:
2858 2789 1030
BbeI G_GCGC'C
Cuts at: 4111 4359 4111
Size: 248 6429
BbsI GAAGACnn'nnnn_
Cuts at: 529 608 1085 1583 1817 1873 2496 2591 529
Size: 79 477 498 234 56 623 95 4615
Fragments arranged by size:
4615 623 498 477 234 95 79 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 139 173 139
Size: 34 6643
BciVI GTATCCnnnnn_n'
Cuts at: 689 4575 5336 6677 689
Size: 3886 761 1341 689
Fragments arranged by size:
3886 1341 761 689
BclI T'GATC_A
Cuts at: 4197 4197
Size: 6677
BfrBI ATG'CAT
Cuts at: 2870 2870
Size: 6677
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 6677
BglII A'GATC_T
Cuts at: 541 1125 2508 4192 6229 541
Size: 584 1383 1684 2037 989
Fragments arranged by size:
2037 1684 1383 989 584
BlpI GC'TnA_GC
Cuts at: 3789 3789
Size: 6677
Bme1580I G_kGCm'C
Cuts at: 4302 4395 5451 4302
Size: 93 1056 5528
Fragments arranged by size:
5528 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1672 3889 3970 4305 6449 1672
Size: 2217 81 335 2144 1900
Fragments arranged by size:
2217 2144 1900 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 563 1544 3081 3474 5044 563
Size: 981 1537 393 1570 2196
Fragments arranged by size:
2196 1570 1537 981 393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 1039 491
Size: 548 6129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 2738 3200 3853 4732 5224 5522 943
Size: 1795 462 653 879 492 298 2098
Fragments arranged by size:
2098 1795 879 653 492 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 3229 6381 3229
Size: 3152 3525
BsaAI yAC'GTr
Cuts at: 4030 4030
Size: 6677
BsaBI GATnn'nnATC
Cuts at: 6 1130 4191 6228 6
Size: 1124 3061 2037 455
Fragments arranged by size:
3061 2037 1124 455
BsaHI Gr'CG_yC
Cuts at: 4108 4356 5058 6554 4108
Size: 248 702 1496 4231
Fragments arranged by size:
4231 1496 702 248
BsaWI w'CCGG_w
Cuts at: 819 3866 4387 5065 5339 5486 5897 819
Size: 3047 521 678 274 147 411 1599
Fragments arranged by size:
3047 1599 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 287 317 287
Size: 30 6647
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1312 2098 3552 1312
Size: 786 1454 4437
Fragments arranged by size:
4437 1454 786
BseYI C'CCAG_C
Cuts at: 700 954 4102 4860 5437 5933 700
Size: 254 3148 758 577 496 1444
Fragments arranged by size:
3148 1444 758 577 496 254
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2312 2312
Size: 6677
BsiEI CG_ry'CG
Cuts at: 104 4219 4265 5473 104
Size: 4115 46 1208 1308
Fragments arranged by size:
4115 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 833 3691 4469 4659 5451 833
Size: 2858 778 190 792 2059
Fragments arranged by size:
2858 2059 792 778 190
BsmI GAATG_Cn'
Cuts at: 1226 2843 3670 1226
Size: 1617 827 4233
Fragments arranged by size:
4233 1617 827
BsmBI CGTCTCn'nnnn_
Cuts at: 405 619 405
Size: 214 6463
Bsp1286I G_dGCh'C
Cuts at: 833 3691 4302 4395 4469 4659 4721 5451 833
Size: 2858 611 93 74 190 62 730 2059
Fragments arranged by size:
2858 2059 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 5897 5897
Size: 6677
BspHI T'CATG_A
Cuts at: 5853 5853
Size: 6677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3093 3727 3941 4243 4624 3093
Size: 634 214 302 381 5146
Fragments arranged by size:
5146 634 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4969 5023 6507 6672 50
Size: 4919 54 1484 165 55
Fragments arranged by size:
4919 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2957 4589 2957
Size: 1632 5045
BsrFI r'CCGG_y
Cuts at: 4675 4856 5065 4675
Size: 181 209 6287
Fragments arranged by size:
6287 209 181
BsrGI T'GTAC_A
Cuts at: 3291 3291
Size: 6677
BssSI C'ACGA_G
Cuts at: 4948 5306 4948
Size: 358 6319
BstAPI GCAn_nnn'nTGC
Cuts at: 4054 4054
Size: 6677
BstBI TT'CG_AA
Cuts at: 5038 5038
Size: 6677
BstXI CCAn_nnnn'nTGG
Cuts at: 255 255
Size: 6677
BstYI r'GATC_y
Cuts at: 1 325 541 1125 2508 2779 3702 4169 4192
Size: 324 216 584 1383 271 923 467 23
Cuts at: 4192 4527 4773 5785 5871 5883 6229 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1383 1012 923 584 467 449 346 335
324 271 246 216 86 23 12
BstZ17I GTA'TAC
Cuts at: 374 374
Size: 6677
Bsu36I CC'TnA_GG
Cuts at: 1016 1448 1016
Size: 432 6245
BtgI C'CryG_G
Cuts at: 377 377
Size: 6677
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2681 4627 4808 4971 2681
Size: 1946 181 163 4387
Fragments arranged by size:
4387 1946 181 163
BtsI GCAGTG_nn'
Cuts at: 591 3920 591
Size: 3329 3348
ClaI AT'CG_AT
Cuts at: 1460 1460
Size: 6677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1094 1129 1094
Size: 35 6642
DraI TTT'AAA
Cuts at: 2120 3676 2120
Size: 1556 5121
DrdI GACnn_nn'nnGTC
Cuts at: 4383 5241 4383
Size: 858 5819
EaeI y'GGCC_r
Cuts at: 2544 4262 4436 4827 4854 6199 2544
Size: 1718 174 391 27 1345 3022
Fragments arranged by size:
3022 1718 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4262 4262
Size: 6677
EarI CTCTTCn'nnn_
Cuts at: 554 584 968 1001 1031 1141 1150 1388 1432
Size: 30 384 33 30 110 9 238 44
Cuts at: 1432 1481 1559 1733 1739 3319 4700 4910 554
Size: 49 78 174 6 1580 1381 210 2321
Fragments arranged by size:
2321 1580 1381 384 238 210 174 110
78 49 44 33 30 30 9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4885 5195 5341 6487 4885
Size: 310 146 1146 5075
Fragments arranged by size:
5075 1146 310 146
EcoICRI GAG'CTC
Cuts at: 831 3689 831
Size: 2858 3819
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 581 835 980 1039 1055 1131 1811 2401
Size: 90 254 145 59 16 76 680 590
Cuts at: 2401 2930 3227 3536 4502 4934 5681 5949 491
Size: 529 297 309 966 432 747 268 1219
Fragments arranged by size:
1219 966 747 680 590 529 432 309
297 268 254 145 90 76 59 16
EcoNI CCTnn'n_nnAGG
Cuts at: 235 235
Size: 6677
EcoO109I rG'GnC_Cy
Cuts at: 280 3816 280
Size: 3536 3141
EcoRI G'AATT_C
Cuts at: 1239 3681 1239
Size: 2442 4235
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 560 4696 560
Size: 4136 2541
FspI TGC'GCA
Cuts at: 4458 4458
Size: 6677
HaeII r_GCGC'y
Cuts at: 612 4111 4359 5381 6360 612
Size: 3499 248 1022 979 929
Fragments arranged by size:
3499 1022 979 929 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1714 1746 1940 1972 2493 2525 3223 3255 4519
Size: 32 194 32 521 32 698 32 1264
Cuts at: 4519 4549 4551 4581 1714
Size: 30 2 30 3810
Fragments arranged by size:
3810 1264 698 521 194 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 356 3716 356
Size: 3360 3317
KasI G'GCGC_C
Cuts at: 4107 4355 4107
Size: 248 6429
KpnI G_GTAC'C
Cuts at: 3278 3697 3278
Size: 419 6258
MfeI C'AATT_G
Cuts at: 253 253
Size: 6677
MluI A'CGCG_T
Cuts at: 6110 6110
Size: 6677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 273 2373 2898 3129 5348 5532 273
Size: 2100 525 231 2219 184 1418
Fragments arranged by size:
2219 2100 1418 525 231 184
MscI TGG'CCA
Cuts at: 2546 4438 2546
Size: 1892 4785
MslI CAynn'nnrTG
Cuts at: 721 1166 1343 1943 3616 4666 4793 721
Size: 445 177 600 1673 1050 127 2605
Fragments arranged by size:
2605 1673 1050 600 445 177 127
MspA1I CmG'CkG
Cuts at: 704 958 1246 2908 3788 4102 4462 5475 5720
Size: 254 288 1662 880 314 360 1013 245
Cuts at: 5720 704
Size: 1661
Fragments arranged by size:
1662 1661 1013 880 360 314 288 254
245
NaeI GCC'GGC
Cuts at: 4858 4858
Size: 6677
NarI GG'CG_CC
Cuts at: 4108 4356 4108
Size: 248 6429
NgoMIV G'CCGG_C
Cuts at: 4856 4856
Size: 6677
NsiI A_TGCA'T
Cuts at: 2872 2872
Size: 6677
NspI r_CATG'y
Cuts at: 2145 3730 4230 5137 2145
Size: 1585 500 907 3685
Fragments arranged by size:
3685 1585 907 500
PciI A'CATG_T
Cuts at: 5133 5133
Size: 6677
PflMI CCAn_nnn'nTGG
Cuts at: 3337 3337
Size: 6677
PmeI GTTT'AAAC
Cuts at: 3676 3676
Size: 6677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5816 5848 5816
Size: 32 6645
PpuMI rG'GwC_Cy
Cuts at: 280 280
Size: 6677
PsiI TTA'TAA
Cuts at: 1067 2527 1067
Size: 1460 5217
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2396 2428 2396
Size: 32 6645
PstI C_TGCA'G
Cuts at: 1638 3399 3724 4409 1638
Size: 1761 325 685 3906
Fragments arranged by size:
3906 1761 685 325
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6677
PvuII CAG'CTG
Cuts at: 704 958 1246 2908 4102 4462 704
Size: 254 288 1662 1194 360 2919
Fragments arranged by size:
2919 1662 1194 360 288 254
RsrII CG'GwC_CG
Cuts at: 4872 4872
Size: 6677
SacI G_AGCT'C
Cuts at: 833 3691 833
Size: 2858 3819
SalI G'TCGA_C
Cuts at: 3714 3714
Size: 6677
SapI GCTCTTCn'nnn_
Cuts at: 4700 4910 4700
Size: 210 6467
SbfI CC_TGCA'GG
Cuts at: 3724 3724
Size: 6677
ScaI AGT'ACT
Cuts at: 1303 6194 1303
Size: 4891 1786
SfcI C'TryA_G
Cuts at: 33 776 1634 2630 3098 3279 3395 3478 3659
Size: 743 858 996 468 181 116 83 181
Cuts at: 3659 3720 4405 5398 5589 33
Size: 61 685 993 191 1121
Fragments arranged by size:
1121 996 993 858 743 685 468 191
181 181 116 83 61
SfoI GGC'GCC
Cuts at: 4109 4357 4109
Size: 248 6429
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6677
SmaI CCC'GGG
Cuts at: 3699 3699
Size: 6677
SmlI C'TyrA_G
Cuts at: 922 1529 2753 3215 3832 4747 5239 5501 922
Size: 607 1224 462 617 915 492 262 2098
Fragments arranged by size:
2098 1224 915 617 607 492 462 262
SpeI A'CTAG_T
Cuts at: 67 2884 67
Size: 2817 3860
SphI G_CATG'C
Cuts at: 3730 4230 3730
Size: 500 6177
SspI AAT'ATT
Cuts at: 1182 1320 2925 3496 1182
Size: 138 1605 571 4363
Fragments arranged by size:
4363 1605 571 138
StyI C'CwwG_G
Cuts at: 1221 2975 3811 4165 1221
Size: 1754 836 354 3733
Fragments arranged by size:
3733 1754 836 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1962 4260 4393 4852 5061 1962
Size: 2298 133 459 209 3578
Fragments arranged by size:
3578 2298 459 209 133
TatI w'GTAC_w
Cuts at: 1301 2412 3291 3445 3651 6192 1301
Size: 1111 879 154 206 2541 1786
Fragments arranged by size:
2541 1786 1111 879 206 154
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1826 1858 6194 6226 1826
Size: 32 4336 32 2277
Fragments arranged by size:
4336 2277 32 32
Tth111I GACn'n_nGTC
Cuts at: 4474 4474
Size: 6677
XbaI T'CTAG_A
Cuts at: 3708 3708
Size: 6677
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4172 4172
Size: 6677
XhoI C'TCGA_G
Cuts at: 1529 1529
Size: 6677
XmaI C'CCGG_G
Cuts at: 3697 3697
Size: 6677
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PciI PflMI PmeI PpiI
PpuMI PsiI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BmgBI BmtI Bpu10I BsiWI
BssHII BstEII DraIII EcoRV FseI FspAI HindIII HpaI
NcoI NdeI NheI NotI NruI PacI PasI PfoI
PmlI PshAI PspOMI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XmnI ZraI