Restriction map for
pF1KE3504
(Circular) (Six-base) MAPSORT of: pF1KE3504.seq Check: 4135 from: 1 to: 5702
pF1KE3504 5702 bp
With 182 enzymes: SgfI *
June 20, 2019 10:05 ..
AccI GT'mk_AC
Cuts at: 239 371 2740 239
Size: 132 2369 3201
Fragments arranged by size:
3201 2369 132
Acc65I G'GTAC_C
Cuts at: 2718 2718
Size: 5702
AclI AA'CG_TT
Cuts at: 5542 5542
Size: 5702
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 1108 1141 1155 1726 3527 3959 4706 4974
Size: 806 33 14 571 1801 432 747 268
Cuts at: 4974 302
Size: 1030
Fragments arranged by size:
1801 1030 806 747 571 432 268 33
14
AflIII A'CryG_T
Cuts at: 333 390 647 2669 3054 4158 5135 333
Size: 57 257 2022 385 1104 977 900
Fragments arranged by size:
2022 1104 977 900 385 257 57
AgeI A'CCGG_T
Cuts at: 4090 4090
Size: 5702
AhdI GACnn_n'nnGTC
Cuts at: 2500 2500
Size: 5702
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1463 1463
Size: 5702
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 882 914 882
Size: 32 5670
AlwNI CAG_nnn'CTG
Cuts at: 2245 3007 4574 2245
Size: 762 1567 3373
Fragments arranged by size:
3373 1567 762
ApaLI G'TGCA_C
Cuts at: 4472 4472
Size: 5702
ApoI r'AATT_y
Cuts at: 444 1183 1423 1456 1900 2706 5055 5311 444
Size: 739 240 33 444 806 2349 256 835
Fragments arranged by size:
2349 835 806 739 444 256 240 33
AseI AT'TA_AT
Cuts at: 20 20
Size: 5702
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5702
AvaI C'yCGr_G
Cuts at: 2722 2722
Size: 5702
BamHI G'GATC_C
Cuts at: 948 2727 948
Size: 1779 3923
BanI G'GyrC_C
Cuts at: 121 2152 2718 3132 3380 3415 121
Size: 2031 566 414 248 35 2408
Fragments arranged by size:
2408 2031 566 414 248 35
BanII G_rGCy'C
Cuts at: 1022 2716 3746 1022
Size: 1694 1030 2978
Fragments arranged by size:
2978 1694 1030
BbeI G_GCGC'C
Cuts at: 125 2156 3136 3384 125
Size: 2031 980 248 2443
Fragments arranged by size:
2443 2031 980 248
BbsI GAAGACnn'nnnn_
Cuts at: 1698 2429 1698
Size: 731 4971
BbvCI CC'TCA_GC
Cuts at: 1042 1042
Size: 5702
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 198 232 90
Size: 34 74 34 5560
Fragments arranged by size:
5560 74 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2681 3600 4361 5702 2681
Size: 919 761 1341 2681
Fragments arranged by size:
2681 1341 919 761
BclI T'GATC_A
Cuts at: 3222 3222
Size: 5702
BfrBI ATG'CAT
Cuts at: 302 1002 302
Size: 700 5002
BglI GCCn_nnn'nGGC
Cuts at: 1624 2161 1624
Size: 537 5165
BglII A'GATC_T
Cuts at: 462 3217 5254 462
Size: 2755 2037 910
Fragments arranged by size:
2755 2037 910
BlpI GC'TnA_GC
Cuts at: 2207 2814 2207
Size: 607 5095
Bme1580I G_kGCm'C
Cuts at: 197 221 1081 1791 3327 3420 4476 197
Size: 24 860 710 1536 93 1056 1423
Fragments arranged by size:
1536 1423 1056 860 710 93 24
BmrI ACTGGGnnnn_n'
Cuts at: 895 1896 2914 2995 3330 5474 895
Size: 1001 1018 81 335 2144 1123
Fragments arranged by size:
2144 1123 1018 1001 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 781 4069 781
Size: 3288 2414
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1006 1800 2270 2276 865
Size: 141 794 470 6 4291
Fragments arranged by size:
4291 794 470 141 6
Bpu10I CC'TnA_GC
Cuts at: 1042 1042
Size: 5702
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2878 3757 4249 4547 2878
Size: 879 492 298 4033
Fragments arranged by size:
4033 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5406 5406
Size: 5702
BsaAI yAC'GTr
Cuts at: 650 1714 3055 650
Size: 1064 1341 3297
Fragments arranged by size:
3297 1341 1064
BsaBI GATnn'nnATC
Cuts at: 6 3216 5253 6
Size: 3210 2037 455
Fragments arranged by size:
3210 2037 455
BsaHI Gr'CG_yC
Cuts at: 122 415 2153 3133 3381 4083 5579 122
Size: 293 1738 980 248 702 1496 245
Fragments arranged by size:
1738 1496 980 702 293 248 245
BsaWI w'CCGG_w
Cuts at: 1314 2891 3412 4090 4364 4511 4922 1314
Size: 1577 521 678 274 147 411 2094
Fragments arranged by size:
2094 1577 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 882 912 2243 2273 882
Size: 30 1331 30 4311
Fragments arranged by size:
4311 1331 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 583 1366 1419 583
Size: 783 53 4866
Fragments arranged by size:
4866 783 53
BseYI C'CCAG_C
Cuts at: 695 3127 3885 4462 4958 695
Size: 2432 758 577 496 1439
Fragments arranged by size:
2432 1439 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1876 1876
Size: 5702
BsiEI CG_ry'CG
Cuts at: 104 3244 3290 4498 104
Size: 3140 46 1208 1308
Fragments arranged by size:
3140 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1358 2388 2716 3494 3684 4476 1358
Size: 1030 328 778 190 792 2584
Fragments arranged by size:
2584 1030 792 778 328 190
BsmI GAATG_Cn'
Cuts at: 1547 2536 1547
Size: 989 4713
Bsp1286I G_dGCh'C
Cuts at: 197 221 1022 1081 1358 1791 2388 2716 3327
Size: 24 801 59 277 433 597 328 611
Cuts at: 3327 3420 3494 3684 3746 4476 197
Size: 93 74 190 62 730 1423
Fragments arranged by size:
1423 801 730 611 597 433 328 277
190 93 74 62 59 24
BspEI T'CCGG_A
Cuts at: 4922 4922
Size: 5702
BspHI T'CATG_A
Cuts at: 4878 4878
Size: 5702
BspMI ACCTGCnnnn'nnnn_
Cuts at: 761 2752 2966 3268 3649 761
Size: 1991 214 302 381 2814
Fragments arranged by size:
2814 1991 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3994 4048 5532 5697 50
Size: 3944 54 1484 165 55
Fragments arranged by size:
3944 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3614 3614
Size: 5702
BsrFI r'CCGG_y
Cuts at: 3700 3881 4090 3700
Size: 181 209 5312
Fragments arranged by size:
5312 209 181
BssSI C'ACGA_G
Cuts at: 3973 4331 3973
Size: 358 5344
BstAPI GCAn_nnn'nTGC
Cuts at: 1139 1553 1804 2245 3079 1139
Size: 414 251 441 834 3762
Fragments arranged by size:
3762 834 441 414 251
BstBI TT'CG_AA
Cuts at: 351 4063 351
Size: 3712 1990
BstXI CCAn_nnnn'nTGG
Cuts at: 650 731 650
Size: 81 5621
BstYI r'GATC_y
Cuts at: 1 462 948 1239 1376 2396 2512 2727 3194
Size: 461 486 291 137 1020 116 215 467
Cuts at: 3194 3217 3552 3798 4810 4896 4908 5254 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1020 1012 486 467 461 449 346 335
291 246 215 137 116 86 23 12
BstZ17I GTA'TAC
Cuts at: 372 372
Size: 5702
BtgI C'CryG_G
Cuts at: 1619 2321 1619
Size: 702 5000
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1532 3652 3833 3996 1532
Size: 2120 181 163 3238
Fragments arranged by size:
3238 2120 181 163
BtsI GCAGTG_nn'
Cuts at: 1791 2945 1791
Size: 1154 4548
ClaI AT'CG_AT
Cuts at: 2066 2066
Size: 5702
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2614 2649 2614
Size: 35 5667
DraI TTT'AAA
Cuts at: 485 2701 485
Size: 2216 3486
DraIII CAC_nnn'GTG
Cuts at: 1473 1473
Size: 5702
DrdI GACnn_nn'nnGTC
Cuts at: 709 790 3408 4266 709
Size: 81 2618 858 2145
Fragments arranged by size:
2618 2145 858 81
EaeI y'GGCC_r
Cuts at: 959 1616 2090 3287 3461 3852 3879 5224 959
Size: 657 474 1197 174 391 27 1345 1437
Fragments arranged by size:
1437 1345 1197 657 474 391 174 27
EagI C'GGCC_G
Cuts at: 3287 3287
Size: 5702
EarI CTCTTCn'nnn_
Cuts at: 2186 3725 3935 2186
Size: 1539 210 3953
Fragments arranged by size:
3953 1539 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3910 4220 4366 5512 3910
Size: 310 146 1146 4100
Fragments arranged by size:
4100 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2714 2714
Size: 5702
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 865 1006 1108 1141 1155 1726 1800 2270
Size: 563 141 102 33 14 571 74 470
Cuts at: 2270 2276 3527 3959 4706 4974 302
Size: 6 1251 432 747 268 1030
Fragments arranged by size:
1251 1030 747 571 563 470 432 268
141 102 74 33 14 6
EcoNI CCTnn'n_nnAGG
Cuts at: 324 1434 2643 324
Size: 1110 1209 3383
Fragments arranged by size:
3383 1209 1110
EcoO109I rG'GnC_Cy
Cuts at: 639 2494 2648 2841 639
Size: 1855 154 193 3500
Fragments arranged by size:
3500 1855 193 154
EcoRI G'AATT_C
Cuts at: 444 1183 1423 1456 2706 444
Size: 739 240 33 1250 3440
Fragments arranged by size:
3440 1250 739 240 33
EcoRV GAT'ATC
Cuts at: 1938 1938
Size: 5702
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 538 828 3721 538
Size: 290 2893 2519
Fragments arranged by size:
2893 2519 290
FspI TGC'GCA
Cuts at: 3483 3483
Size: 5702
HaeII r_GCGC'y
Cuts at: 117 125 2156 3136 3384 4406 5385 117
Size: 8 2031 980 248 1022 979 434
Fragments arranged by size:
2031 1022 980 979 434 248 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2059 2091 2486 2518 3544 3574 3576 3606 2059
Size: 32 395 32 1026 30 2 30 4155
Fragments arranged by size:
4155 1026 395 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2741 2741
Size: 5702
KasI G'GCGC_C
Cuts at: 121 2152 3132 3380 121
Size: 2031 980 248 2443
Fragments arranged by size:
2443 2031 980 248
KpnI G_GTAC'C
Cuts at: 2722 2722
Size: 5702
MfeI C'AATT_G
Cuts at: 590 2044 590
Size: 1454 4248
MluI A'CGCG_T
Cuts at: 5135 5135
Size: 5702
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 712 1267 4373 4557 712
Size: 555 3106 184 1857
Fragments arranged by size:
3106 1857 555 184
MscI TGG'CCA
Cuts at: 961 1618 3463 961
Size: 657 1845 3200
Fragments arranged by size:
3200 1845 657
MslI CAynn'nnrTG
Cuts at: 1001 1264 2320 3691 3818 1001
Size: 263 1056 1371 127 2885
Fragments arranged by size:
2885 1371 1056 263 127
MspA1I CmG'CkG
Cuts at: 149 699 1139 2088 2109 2214 2813 3127 3487
Size: 550 440 949 21 105 599 314 360
Cuts at: 3487 4500 4745 149
Size: 1013 245 1106
Fragments arranged by size:
1106 1013 949 599 550 440 360 314
245 105 21
NaeI GCC'GGC
Cuts at: 3883 3883
Size: 5702
NarI GG'CG_CC
Cuts at: 122 2153 3133 3381 122
Size: 2031 980 248 2443
Fragments arranged by size:
2443 2031 980 248
NcoI C'CATG_G
Cuts at: 1619 2321 1619
Size: 702 5000
NdeI CA'TA_TG
Cuts at: 998 2523 998
Size: 1525 4177
NgoMIV G'CCGG_C
Cuts at: 3881 3881
Size: 5702
NsiI A_TGCA'T
Cuts at: 304 1004 304
Size: 700 5002
NspI r_CATG'y
Cuts at: 138 204 337 378 394 841 1392 2120 2673
Size: 66 133 41 16 447 551 728 553
Cuts at: 2673 2755 3255 4162 138
Size: 82 500 907 1678
Fragments arranged by size:
1678 907 728 553 551 500 447 133
82 66 41 16
PciI A'CATG_T
Cuts at: 333 390 2669 4158 333
Size: 57 2279 1489 1877
Fragments arranged by size:
2279 1877 1489 57
PflMI CCAn_nnn'nTGG
Cuts at: 1099 1099
Size: 5702
PfoI T'CCnGG_A
Cuts at: 1022 1067 1022
Size: 45 5657
PmeI GTTT'AAAC
Cuts at: 2701 2701
Size: 5702
PmlI CAC'GTG
Cuts at: 650 650
Size: 5702
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 882 914 1363 1395 4841 4873 882
Size: 32 449 32 3446 32 1711
Fragments arranged by size:
3446 1711 449 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 639 2494 639
Size: 1855 3847
PstI C_TGCA'G
Cuts at: 171 2749 3434 171
Size: 2578 685 2439
Fragments arranged by size:
2578 2439 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5702
PvuII CAG'CTG
Cuts at: 699 1139 2088 3127 3487 699
Size: 440 949 1039 360 2914
Fragments arranged by size:
2914 1039 949 440 360
RsrII CG'GwC_CG
Cuts at: 3897 3897
Size: 5702
SacI G_AGCT'C
Cuts at: 2716 2716
Size: 5702
SalI G'TCGA_C
Cuts at: 2739 2739
Size: 5702
SapI GCTCTTCn'nnn_
Cuts at: 2186 3725 3935 2186
Size: 1539 210 3953
Fragments arranged by size:
3953 1539 210
SbfI CC_TGCA'GG
Cuts at: 2749 2749
Size: 5702
ScaI AGT'ACT
Cuts at: 5219 5219
Size: 5702
SexAI A'CCwGG_T
Cuts at: 1469 1469
Size: 5702
SfcI C'TryA_G
Cuts at: 33 167 2745 3430 4423 4614 33
Size: 134 2578 685 993 191 1121
Fragments arranged by size:
2578 1121 993 685 191 134
SfoI GGC'GCC
Cuts at: 123 2154 3134 3382 123
Size: 2031 980 248 2443
Fragments arranged by size:
2443 2031 980 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5702
SmaI CCC'GGG
Cuts at: 2724 2724
Size: 5702
SmlI C'TyrA_G
Cuts at: 2857 3772 4264 4526 2857
Size: 915 492 262 4033
Fragments arranged by size:
4033 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5702
SphI G_CATG'C
Cuts at: 138 841 2755 3255 138
Size: 703 1914 500 2585
Fragments arranged by size:
2585 1914 703 500
SspI AAT'ATT
Cuts at: 564 1220 564
Size: 656 5046
StuI AGG'CCT
Cuts at: 1399 1747 1399
Size: 348 5354
StyI C'CwwG_G
Cuts at: 1619 2321 2836 3190 1619
Size: 702 515 354 4131
Fragments arranged by size:
4131 702 515 354
SwaI ATTT'AAAT
Cuts at: 485 485
Size: 5702
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 709 2598 3285 3418 3877 4086 709
Size: 1889 687 133 459 209 2325
Fragments arranged by size:
2325 1889 687 459 209 133
TatI w'GTAC_w
Cuts at: 2132 2672 5217 2132
Size: 540 2545 2617
Fragments arranged by size:
2617 2545 540
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2594 2626 5219 5251 2594
Size: 32 2593 32 3045
Fragments arranged by size:
3045 2593 32 32
Tth111I GACn'n_nGTC
Cuts at: 235 3499 235
Size: 3264 2438
XbaI T'CTAG_A
Cuts at: 465 2733 465
Size: 2268 3434
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 703 3197 703
Size: 2494 3208
XmaI C'CCGG_G
Cuts at: 2722 2722
Size: 5702
XmnI GAAnn'nnTTC
Cuts at: 1547 1982 1547
Size: 435 5267
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI ApaI AscI AvrII
BaeI BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstEII
Bsu36I FseI FspAI HindIII HpaI NheI NotI NruI
PacI PasI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI XhoI ZraI