Restriction map for
pF1KE3523
(Circular) (Six-base) MAPSORT of: pF1KE3523.seq Check: 1488 from: 1 to: 4160
pF1KE3523 4160 bp
With 182 enzymes: SgfI *
November 18, 2016 17:23 ..
AccI GT'mk_AC
Cuts at: 1198 1198
Size: 4160
Acc65I G'GTAC_C
Cuts at: 746 1176 746
Size: 430 3730
AclI AA'CG_TT
Cuts at: 4000 4000
Size: 4160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 710 1049 1055 1985 2417 3164 3432 433
Size: 277 339 6 930 432 747 268 1161
Fragments arranged by size:
1161 930 747 432 339 277 268 6
AflIII A'CryG_T
Cuts at: 609 1512 2616 3593 609
Size: 903 1104 977 1176
Fragments arranged by size:
1176 1104 977 903
AgeI A'CCGG_T
Cuts at: 2548 2548
Size: 4160
AlwNI CAG_nnn'CTG
Cuts at: 1465 3032 1465
Size: 1567 2593
ApaLI G'TGCA_C
Cuts at: 2930 2930
Size: 4160
ApoI r'AATT_y
Cuts at: 1164 3513 3769 1164
Size: 2349 256 1555
Fragments arranged by size:
2349 1555 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4160
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
AvaI C'yCGr_G
Cuts at: 692 1180 692
Size: 488 3672
BamHI G'GATC_C
Cuts at: 1185 1185
Size: 4160
BanI G'GyrC_C
Cuts at: 746 1176 1590 1838 1873 746
Size: 430 414 248 35 3033
Fragments arranged by size:
3033 430 414 248 35
BanII G_rGCy'C
Cuts at: 668 1174 2204 668
Size: 506 1030 2624
Fragments arranged by size:
2624 1030 506
BbeI G_GCGC'C
Cuts at: 1594 1842 1594
Size: 248 3912
BbsI GAAGACnn'nnnn_
Cuts at: 1043 1043
Size: 4160
BciVI GTATCCnnnnn_n'
Cuts at: 915 2058 2819 4160 915
Size: 1143 761 1341 915
Fragments arranged by size:
1341 1143 915 761
BclI T'GATC_A
Cuts at: 1680 1680
Size: 4160
BglII A'GATC_T
Cuts at: 1675 3712 1675
Size: 2037 2123
BlpI GC'TnA_GC
Cuts at: 1272 1272
Size: 4160
Bme1580I G_kGCm'C
Cuts at: 150 1785 1878 2934 150
Size: 1635 93 1056 1376
Fragments arranged by size:
1635 1376 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 507 1372 1453 1788 3932 507
Size: 865 81 335 2144 735
Fragments arranged by size:
2144 865 735 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 4160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 904 904
Size: 4160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1336 2215 2707 3005 1336
Size: 879 492 298 2491
Fragments arranged by size:
2491 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1067 3864 1067
Size: 2797 1363
BsaAI yAC'GTr
Cuts at: 1513 1513
Size: 4160
BsaBI GATnn'nnATC
Cuts at: 6 1674 3711 6
Size: 1668 2037 455
Fragments arranged by size:
2037 1668 455
BsaHI Gr'CG_yC
Cuts at: 1591 1839 2541 4037 1591
Size: 248 702 1496 1714
Fragments arranged by size:
1714 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1349 1870 2548 2822 2969 3380 1349
Size: 521 678 274 147 411 2129
Fragments arranged by size:
2129 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 168 198 1030 1060 1066 1096 168
Size: 30 832 30 6 30 3232
Fragments arranged by size:
3232 832 30 30 30 6
BseYI C'CCAG_C
Cuts at: 279 709 768 903 1014 1585 2343 2920 3416
Size: 430 59 135 111 571 758 577 496
Cuts at: 3416 279
Size: 1023
Fragments arranged by size:
1023 758 577 571 496 430 135 111
59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 830 830
Size: 4160
BsiEI CG_ry'CG
Cuts at: 104 1702 1748 2956 104
Size: 1598 46 1208 1308
Fragments arranged by size:
1598 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 443 668 1174 1952 2142 2934 443
Size: 225 506 778 190 792 1669
Fragments arranged by size:
1669 792 778 506 225 190
BsmI GAATG_Cn'
Cuts at: 369 369
Size: 4160
BsmBI CGTCTCn'nnnn_
Cuts at: 1086 1086
Size: 4160
Bsp1286I G_dGCh'C
Cuts at: 150 443 668 1174 1785 1878 1952 2142 2204
Size: 293 225 506 611 93 74 190 62
Cuts at: 2204 2934 150
Size: 730 1376
Fragments arranged by size:
1376 730 611 506 293 225 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3380 3380
Size: 4160
BspHI T'CATG_A
Cuts at: 1104 3336 1104
Size: 2232 1928
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1210 1424 1726 2107 1210
Size: 214 302 381 3263
Fragments arranged by size:
3263 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2452 2506 3990 4155 50
Size: 2402 54 1484 165 55
Fragments arranged by size:
2402 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2072 2072
Size: 4160
BsrFI r'CCGG_y
Cuts at: 749 2158 2339 2548 749
Size: 1409 181 209 2361
Fragments arranged by size:
2361 1409 209 181
BssSI C'ACGA_G
Cuts at: 2431 2789 2431
Size: 358 3802
BstAPI GCAn_nnn'nTGC
Cuts at: 1537 1537
Size: 4160
BstBI TT'CG_AA
Cuts at: 2521 2521
Size: 4160
BstYI r'GATC_y
Cuts at: 1 204 1185 1652 1675 2010 2256 3268 3354
Size: 203 981 467 23 335 246 1012 86
Cuts at: 3354 3366 3712 1
Size: 12 346 449
Fragments arranged by size:
1012 981 467 449 346 335 246 203
86 23 12
BtgI C'CryG_G
Cuts at: 172 172
Size: 4160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2110 2291 2454 2110
Size: 181 163 3816
Fragments arranged by size:
3816 181 163
BtsI GCAGTG_nn'
Cuts at: 973 1403 973
Size: 430 3730
DraI TTT'AAA
Cuts at: 347 1159 347
Size: 812 3348
DrdI GACnn_nn'nnGTC
Cuts at: 1866 2724 1866
Size: 858 3302
EaeI y'GGCC_r
Cuts at: 928 1745 1919 2310 2337 3682 928
Size: 817 174 391 27 1345 1406
Fragments arranged by size:
1406 1345 817 391 174 27
EagI C'GGCC_G
Cuts at: 1745 1745
Size: 4160
EarI CTCTTCn'nnn_
Cuts at: 194 226 729 2183 2393 194
Size: 32 503 1454 210 1961
Fragments arranged by size:
1961 1454 503 210 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1074 2368 2678 2824 3970 1074
Size: 1294 310 146 1146 1264
Fragments arranged by size:
1294 1264 1146 310 146
EcoICRI GAG'CTC
Cuts at: 666 1172 666
Size: 506 3654
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 710 904 1049 1055 1985 2417 3164 3432
Size: 277 194 145 6 930 432 747 268
Cuts at: 3432 433
Size: 1161
Fragments arranged by size:
1161 930 747 432 277 268 194 145
6
EcoO109I rG'GnC_Cy
Cuts at: 585 624 1299 585
Size: 39 675 3446
Fragments arranged by size:
3446 675 39
EcoRI G'AATT_C
Cuts at: 1164 1164
Size: 4160
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 4160
FspI TGC'GCA
Cuts at: 1941 1941
Size: 4160
HaeII r_GCGC'y
Cuts at: 1121 1594 1842 2864 3843 1121
Size: 473 248 1022 979 1438
Fragments arranged by size:
1438 1022 979 473 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1030 1062 1064 1066 1096 1098 2002 2032 2034
Size: 32 2 2 30 2 904 30 2
Cuts at: 2034 2064 1030
Size: 30 3126
Fragments arranged by size:
3126 904 32 30 30 30 2 2
2 2
HincII GTy'rAC
Cuts at: 844 1199 844
Size: 355 3805
HindIII A'AGCT_T
Cuts at: 401 401
Size: 4160
KasI G'GCGC_C
Cuts at: 1590 1838 1590
Size: 248 3912
KpnI G_GTAC'C
Cuts at: 750 1180 750
Size: 430 3730
MluI A'CGCG_T
Cuts at: 3593 3593
Size: 4160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 890 2831 3015 890
Size: 1941 184 2035
Fragments arranged by size:
2035 1941 184
MscI TGG'CCA
Cuts at: 1921 1921
Size: 4160
MslI CAynn'nnrTG
Cuts at: 358 2149 2276 358
Size: 1791 127 2242
Fragments arranged by size:
2242 1791 127
MspA1I CmG'CkG
Cuts at: 882 1134 1271 1585 1945 2958 3203 882
Size: 252 137 314 360 1013 245 1839
Fragments arranged by size:
1839 1013 360 314 252 245 137
NaeI GCC'GGC
Cuts at: 2341 2341
Size: 4160
NarI GG'CG_CC
Cuts at: 1591 1839 1591
Size: 248 3912
NdeI CA'TA_TG
Cuts at: 802 802
Size: 4160
NgoMIV G'CCGG_C
Cuts at: 2339 2339
Size: 4160
NspI r_CATG'y
Cuts at: 430 498 1213 1713 2620 430
Size: 68 715 500 907 1970
Fragments arranged by size:
1970 907 715 500 68
PasI CC'CwG_GG
Cuts at: 125 125
Size: 4160
PciI A'CATG_T
Cuts at: 2616 2616
Size: 4160
PfoI T'CCnGG_A
Cuts at: 531 531
Size: 4160
PmeI GTTT'AAAC
Cuts at: 1159 1159
Size: 4160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 4128
PpuMI rG'GwC_Cy
Cuts at: 624 624
Size: 4160
PstI C_TGCA'G
Cuts at: 1207 1892 1207
Size: 685 3475
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4160
PvuII CAG'CTG
Cuts at: 882 1134 1585 1945 882
Size: 252 451 360 3097
Fragments arranged by size:
3097 451 360 252
RsrII CG'GwC_CG
Cuts at: 2355 2355
Size: 4160
SacI G_AGCT'C
Cuts at: 668 1174 668
Size: 506 3654
SalI G'TCGA_C
Cuts at: 1197 1197
Size: 4160
SapI GCTCTTCn'nnn_
Cuts at: 226 2183 2393 226
Size: 1957 210 1993
Fragments arranged by size:
1993 1957 210
SbfI CC_TGCA'GG
Cuts at: 1207 1207
Size: 4160
ScaI AGT'ACT
Cuts at: 3677 3677
Size: 4160
SfcI C'TryA_G
Cuts at: 33 914 1203 1888 2881 3072 33
Size: 881 289 685 993 191 1121
Fragments arranged by size:
1121 993 881 685 289 191
SfoI GGC'GCC
Cuts at: 1592 1840 1592
Size: 248 3912
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
SmaI CCC'GGG
Cuts at: 694 1182 694
Size: 488 3672
SmlI C'TyrA_G
Cuts at: 1315 2230 2722 2984 1315
Size: 915 492 262 2491
Fragments arranged by size:
2491 915 492 262
SpeI A'CTAG_T
Cuts at: 67 336 67
Size: 269 3891
SphI G_CATG'C
Cuts at: 1213 1713 1213
Size: 500 3660
SrfI GCCC'GGGC
Cuts at: 694 694
Size: 4160
SspI AAT'ATT
Cuts at: 435 784 435
Size: 349 3811
StyI C'CwwG_G
Cuts at: 1294 1648 1294
Size: 354 3806
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 271 1743 1876 2335 2544 271
Size: 1472 133 459 209 1887
Fragments arranged by size:
1887 1472 459 209 133
TatI w'GTAC_w
Cuts at: 3675 3675
Size: 4160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 168 200 323 355 3677 3709 168
Size: 32 123 32 3322 32 619
Fragments arranged by size:
3322 619 123 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1078 1957 1078
Size: 879 3281
XbaI T'CTAG_A
Cuts at: 1191 1191
Size: 4160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1655 1655
Size: 4160
XmaI C'CCGG_G
Cuts at: 692 1180 692
Size: 488 3672
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BseRI BsiWI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NcoI NheI NotI NruI
NsiI PacI PflMI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI StuI
SwaI XhoI XmnI ZraI