Restriction map for
pF1KE3532
(Circular) (Six-base) MAPSORT of: pF1KE3532.seq Check: 7790 from: 1 to: 4364
pF1KE3532 4364 bp
With 182 enzymes: SgfI *
August 29, 2012 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 174 174
Size: 4364
AccI GT'mk_AC
Cuts at: 1402 1402
Size: 4364
Acc65I G'GTAC_C
Cuts at: 1380 1380
Size: 4364
AclI AA'CG_TT
Cuts at: 4204 4204
Size: 4364
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2189 2621 3368 3636 2189
Size: 432 747 268 2917
Fragments arranged by size:
2917 747 432 268
AflIII A'CryG_T
Cuts at: 1716 2820 3797 1716
Size: 1104 977 2283
Fragments arranged by size:
2283 1104 977
AgeI A'CCGG_T
Cuts at: 2752 2752
Size: 4364
AleI CACnn'nnGTG
Cuts at: 1187 1187
Size: 4364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1067 1067
Size: 4364
AlwNI CAG_nnn'CTG
Cuts at: 1017 1669 3236 1017
Size: 652 1567 2145
Fragments arranged by size:
2145 1567 652
ApaI G_GGCC'C
Cuts at: 383 618 383
Size: 235 4129
ApaLI G'TGCA_C
Cuts at: 997 3134 997
Size: 2137 2227
ApoI r'AATT_y
Cuts at: 1368 3717 3973 1368
Size: 2349 256 1759
Fragments arranged by size:
2349 1759 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4364
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
AvaI C'yCGr_G
Cuts at: 1384 1384
Size: 4364
AvrII C'CTAG_G
Cuts at: 1240 1240
Size: 4364
BamHI G'GATC_C
Cuts at: 1281 1389 1281
Size: 108 4256
BanI G'GyrC_C
Cuts at: 132 150 596 708 775 1139 1380 1794 2042
Size: 18 446 112 67 364 241 414 248
Cuts at: 2042 2077 132
Size: 35 2419
Fragments arranged by size:
2419 446 414 364 248 241 112 67
35 18
BanII G_rGCy'C
Cuts at: 383 618 1378 2408 383
Size: 235 760 1030 2339
Fragments arranged by size:
2339 1030 760 235
BbeI G_GCGC'C
Cuts at: 600 712 779 1798 2046 600
Size: 112 67 1019 248 2918
Fragments arranged by size:
2918 1019 248 112 67
BciVI GTATCCnnnnn_n'
Cuts at: 2262 3023 4364 2262
Size: 761 1341 2262
Fragments arranged by size:
2262 1341 761
BclI T'GATC_A
Cuts at: 1884 1884
Size: 4364
BglI GCCn_nnn'nGGC
Cuts at: 393 706 802 393
Size: 313 96 3955
Fragments arranged by size:
3955 313 96
BglII A'GATC_T
Cuts at: 1879 3916 1879
Size: 2037 2327
BlpI GC'TnA_GC
Cuts at: 1476 1476
Size: 4364
Bme1580I G_kGCm'C
Cuts at: 266 383 606 618 1001 1088 1989 2082 3138
Size: 117 223 12 383 87 901 93 1056
Cuts at: 3138 266
Size: 1492
Fragments arranged by size:
1492 1056 901 383 223 117 93 87
12
BmgBI CAC'GTC
Cuts at: 1189 1189
Size: 4364
BmrI ACTGGGnnnn_n'
Cuts at: 1220 1576 1657 1992 4136 1220
Size: 356 81 335 2144 1448
Fragments arranged by size:
2144 1448 356 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 532 1122 2731 532
Size: 590 1609 2165
Fragments arranged by size:
2165 1609 590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 751
Size: 4364
Bpu10I CC'TnA_GC
Cuts at: 1018 1018
Size: 4364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1540 2419 2911 3209 1540
Size: 879 492 298 2695
Fragments arranged by size:
2695 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4068 4068
Size: 4364
BsaAI yAC'GTr
Cuts at: 1717 1717
Size: 4364
BsaBI GATnn'nnATC
Cuts at: 6 1878 3915 6
Size: 1872 2037 455
Fragments arranged by size:
2037 1872 455
BsaHI Gr'CG_yC
Cuts at: 597 709 776 1795 2043 2745 4241 597
Size: 112 67 1019 248 702 1496 720
Fragments arranged by size:
1496 1019 720 702 248 112 67
BsaWI w'CCGG_w
Cuts at: 1553 2074 2752 3026 3173 3584 1553
Size: 521 678 274 147 411 2333
Fragments arranged by size:
2333 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1297 1297
Size: 4364
BseYI C'CCAG_C
Cuts at: 236 1081 1789 2547 3124 3620 236
Size: 845 708 758 577 496 980
Fragments arranged by size:
980 845 758 708 577 496
BsiEI CG_ry'CG
Cuts at: 104 496 764 1906 1952 3160 104
Size: 392 268 1142 46 1208 1308
Fragments arranged by size:
1308 1208 1142 392 268 46
BsiHKAI G_wGCw'C
Cuts at: 587 1001 1024 1118 1135 1378 2156 2346 3138
Size: 414 23 94 17 243 778 190 792
Cuts at: 3138 587
Size: 1813
Fragments arranged by size:
1813 792 778 414 243 190 94 23
17
BsmBI CGTCTCn'nnnn_
Cuts at: 277 277
Size: 4364
Bsp1286I G_dGCh'C
Cuts at: 266 383 587 606 618 1001 1024 1088 1118
Size: 117 204 19 12 383 23 64 30
Cuts at: 1118 1135 1378 1989 2082 2156 2346 2408 3138
Size: 17 243 611 93 74 190 62 730
Cuts at: 3138 266
Size: 1492
Fragments arranged by size:
1492 730 611 383 243 204 190 117
93 74 64 62 30 23 19 17
12
BspEI T'CCGG_A
Cuts at: 3584 3584
Size: 4364
BspHI T'CATG_A
Cuts at: 3540 3540
Size: 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 174 1035 1414 1628 1930 2311 174
Size: 861 379 214 302 381 2227
Fragments arranged by size:
2227 861 381 379 302 214
BsrBI CCG'CTC
Cuts at: 50 2656 2710 4194 4359 50
Size: 2606 54 1484 165 55
Fragments arranged by size:
2606 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2276 2276
Size: 4364
BsrFI r'CCGG_y
Cuts at: 845 1001 1314 2362 2543 2752 845
Size: 156 313 1048 181 209 2457
Fragments arranged by size:
2457 1048 313 209 181 156
BssHII G'CGCG_C
Cuts at: 665 665
Size: 4364
BssSI C'ACGA_G
Cuts at: 2635 2993 2635
Size: 358 4006
BstAPI GCAn_nnn'nTGC
Cuts at: 1139 1741 1139
Size: 602 3762
BstBI TT'CG_AA
Cuts at: 2725 2725
Size: 4364
BstXI CCAn_nnnn'nTGG
Cuts at: 375 978 1285 375
Size: 603 307 3454
Fragments arranged by size:
3454 603 307
BstYI r'GATC_y
Cuts at: 1 1281 1389 1856 1879 2214 2460 3472 3558
Size: 1280 108 467 23 335 246 1012 86
Cuts at: 3558 3570 3916 1
Size: 12 346 449
Fragments arranged by size:
1280 1012 467 449 346 335 246 108
86 23 12
BtgI C'CryG_G
Cuts at: 107 779 107
Size: 672 3692
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 781 2314 2495 2658 781
Size: 1533 181 163 2487
Fragments arranged by size:
2487 1533 181 163
BtsI GCAGTG_nn'
Cuts at: 1607 1607
Size: 4364
DraI TTT'AAA
Cuts at: 1363 1363
Size: 4364
DraIII CAC_nnn'GTG
Cuts at: 1139 1139
Size: 4364
DrdI GACnn_nn'nnGTC
Cuts at: 2070 2928 2070
Size: 858 3506
EaeI y'GGCC_r
Cuts at: 493 761 1154 1312 1949 2123 2514 2541 3886
Size: 268 393 158 637 174 391 27 1345
Cuts at: 3886 493
Size: 971
Fragments arranged by size:
1345 971 637 393 391 268 174 158
27
EagI C'GGCC_G
Cuts at: 493 761 1949 493
Size: 268 1188 2908
Fragments arranged by size:
2908 1188 268
EarI CTCTTCn'nnn_
Cuts at: 223 340 952 2387 2597 223
Size: 117 612 1435 210 1990
Fragments arranged by size:
1990 1435 612 210 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 907 2572 2882 3028 4174 907
Size: 1665 310 146 1146 1097
Fragments arranged by size:
1665 1146 1097 310 146
EcoICRI GAG'CTC
Cuts at: 1376 1376
Size: 4364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 2189 2621 3368 3636 751
Size: 1438 432 747 268 1479
Fragments arranged by size:
1479 1438 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 379 380 530 542 615 1014 1222 1244 1503
Size: 1 150 12 73 399 208 22 259
Cuts at: 1503 379
Size: 3240
Fragments arranged by size:
3240 399 259 208 150 73 22 12
1
EcoRI G'AATT_C
Cuts at: 1368 1368
Size: 4364
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4364
FspI TGC'GCA
Cuts at: 2145 2145
Size: 4364
HaeII r_GCGC'y
Cuts at: 600 712 779 1798 2046 3068 4047 600
Size: 112 67 1019 248 1022 979 917
Fragments arranged by size:
1022 1019 979 917 248 112 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2206 2236 2238 2268 2206
Size: 30 2 30 4302
Fragments arranged by size:
4302 30 30 2
HincII GTy'rAC
Cuts at: 1403 1403
Size: 4364
KasI G'GCGC_C
Cuts at: 596 708 775 1794 2042 596
Size: 112 67 1019 248 2918
Fragments arranged by size:
2918 1019 248 112 67
KpnI G_GTAC'C
Cuts at: 1384 1384
Size: 4364
MluI A'CGCG_T
Cuts at: 3797 3797
Size: 4364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3035 3219 3035
Size: 184 4180
MscI TGG'CCA
Cuts at: 2125 2125
Size: 4364
MslI CAynn'nnrTG
Cuts at: 1187 2353 2480 1187
Size: 1166 127 3071
Fragments arranged by size:
3071 1166 127
MspA1I CmG'CkG
Cuts at: 190 1475 1789 2149 3162 3407 190
Size: 1285 314 360 1013 245 1147
Fragments arranged by size:
1285 1147 1013 360 314 245
NaeI GCC'GGC
Cuts at: 847 1316 2545 847
Size: 469 1229 2666
Fragments arranged by size:
2666 1229 469
NarI GG'CG_CC
Cuts at: 597 709 776 1795 2043 597
Size: 112 67 1019 248 2918
Fragments arranged by size:
2918 1019 248 112 67
NcoI C'CATG_G
Cuts at: 107 779 107
Size: 672 3692
NgoMIV G'CCGG_C
Cuts at: 845 1314 2543 845
Size: 469 1229 2666
Fragments arranged by size:
2666 1229 469
NotI GC'GGCC_GC
Cuts at: 761 761
Size: 4364
NspI r_CATG'y
Cuts at: 1417 1917 2824 1417
Size: 500 907 2957
Fragments arranged by size:
2957 907 500
PasI CC'CwG_GG
Cuts at: 177 611 177
Size: 434 3930
PciI A'CATG_T
Cuts at: 2820 2820
Size: 4364
PmeI GTTT'AAAC
Cuts at: 1363 1363
Size: 4364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 3503
Size: 32 4332
PpuMI rG'GwC_Cy
Cuts at: 1014 1014
Size: 4364
PspOMI G'GGCC_C
Cuts at: 379 614 379
Size: 235 4129
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 907 939 907
Size: 32 4332
PstI C_TGCA'G
Cuts at: 425 1411 2096 425
Size: 986 685 2693
Fragments arranged by size:
2693 986 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4364
PvuII CAG'CTG
Cuts at: 190 1789 2149 190
Size: 1599 360 2405
Fragments arranged by size:
2405 1599 360
RsrII CG'GwC_CG
Cuts at: 2559 2559
Size: 4364
SacI G_AGCT'C
Cuts at: 1378 1378
Size: 4364
SalI G'TCGA_C
Cuts at: 1401 1401
Size: 4364
SapI GCTCTTCn'nnn_
Cuts at: 223 2387 2597 223
Size: 2164 210 1990
Fragments arranged by size:
2164 1990 210
SbfI CC_TGCA'GG
Cuts at: 1411 1411
Size: 4364
ScaI AGT'ACT
Cuts at: 3881 3881
Size: 4364
SexAI A'CCwGG_T
Cuts at: 896 926 1135 896
Size: 30 209 4125
Fragments arranged by size:
4125 209 30
SfcI C'TryA_G
Cuts at: 33 421 1407 2092 3085 3276 33
Size: 388 986 685 993 191 1121
Fragments arranged by size:
1121 993 986 685 388 191
SfoI GGC'GCC
Cuts at: 598 710 777 1796 2044 598
Size: 112 67 1019 248 2918
Fragments arranged by size:
2918 1019 248 112 67
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
SmaI CCC'GGG
Cuts at: 1386 1386
Size: 4364
SmlI C'TyrA_G
Cuts at: 1519 2434 2926 3188 1519
Size: 915 492 262 2695
Fragments arranged by size:
2695 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4364
SphI G_CATG'C
Cuts at: 1417 1917 1417
Size: 500 3864
StyI C'CwwG_G
Cuts at: 107 479 737 779 830 1240 1498 1852 107
Size: 372 258 42 51 410 258 354 2619
Fragments arranged by size:
2619 410 372 354 258 258 51 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 760 1049 1947 2080 2539 2748 760
Size: 289 898 133 459 209 2376
Fragments arranged by size:
2376 898 459 289 209 133
TatI w'GTAC_w
Cuts at: 3879 3879
Size: 4364
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3881 3913 3881
Size: 32 4332
Tth111I GACn'n_nGTC
Cuts at: 2161 2161
Size: 4364
XbaI T'CTAG_A
Cuts at: 1395 1395
Size: 4364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 975 978 1036 1859 975
Size: 3 58 823 3480
Fragments arranged by size:
3480 823 58 3
XmaI C'CCGG_G
Cuts at: 1384 1384
Size: 4364
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PasI PciI PmeI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI BaeI BbsI
BbvCI BcgI BfrBI BmtI BsaXI BsgI BsiWI BsmI
BsrGI BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NruI
NsiI PacI PflMI PfoI PmlI PshAI PsiI PspXI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI