Restriction map for
pF1KE3542
(Circular) (Six-base) MAPSORT of: pF1KE3542.seq Check: 2449 from: 1 to: 6503
pF1KE3542 6503 bp
With 182 enzymes: SgfI *
March 22, 2013 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 192 340 1794 2296 192
Size: 148 1454 502 4399
Fragments arranged by size:
4399 1454 502 148
AccI GT'mk_AC
Cuts at: 823 3541 823
Size: 2718 3785
Acc65I G'GTAC_C
Cuts at: 3519 3519
Size: 6503
AclI AA'CG_TT
Cuts at: 6343 6343
Size: 6503
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1130 1370 4328 4760 5507 5775 1130
Size: 240 2958 432 747 268 1858
Fragments arranged by size:
2958 1858 747 432 268 240
AflII C'TTAA_G
Cuts at: 1903 1903
Size: 6503
AflIII A'CryG_T
Cuts at: 3855 4959 5936 3855
Size: 1104 977 4422
Fragments arranged by size:
4422 1104 977
AgeI A'CCGG_T
Cuts at: 4891 4891
Size: 6503
AhdI GACnn_n'nnGTC
Cuts at: 1605 3433 1605
Size: 1828 4675
AleI CACnn'nnGTG
Cuts at: 3217 3217
Size: 6503
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1802 1802
Size: 6503
AlwNI CAG_nnn'CTG
Cuts at: 2875 3808 5375 2875
Size: 933 1567 4003
Fragments arranged by size:
4003 1567 933
ApaLI G'TGCA_C
Cuts at: 5273 5273
Size: 6503
ApoI r'AATT_y
Cuts at: 412 686 703 805 905 1975 1981 2251 2279
Size: 274 17 102 100 1070 6 270 28
Cuts at: 2279 2311 2944 3154 3507 5856 6112 412
Size: 32 633 210 353 2349 256 803
Fragments arranged by size:
2349 1070 803 633 353 274 270 256
210 102 100 32 28 17 6
AseI AT'TA_AT
Cuts at: 20 20
Size: 6503
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6503
AvaI C'yCGr_G
Cuts at: 220 244 2190 3523 220
Size: 24 1946 1333 3200
Fragments arranged by size:
3200 1946 1333 24
AvrII C'CTAG_G
Cuts at: 379 1393 379
Size: 1014 5489
BamHI G'GATC_C
Cuts at: 2659 3528 2659
Size: 869 5634
BanI G'GyrC_C
Cuts at: 147 239 3519 3933 4181 4216 147
Size: 92 3280 414 248 35 2434
Fragments arranged by size:
3280 2434 414 248 92 35
BanII G_rGCy'C
Cuts at: 142 1447 3517 4547 142
Size: 1305 2070 1030 2098
Fragments arranged by size:
2098 2070 1305 1030
BbeI G_GCGC'C
Cuts at: 151 243 3937 4185 151
Size: 92 3694 248 2469
Fragments arranged by size:
3694 2469 248 92
BbsI GAAGACnn'nnnn_
Cuts at: 302 2234 2347 302
Size: 1932 113 4458
Fragments arranged by size:
4458 1932 113
BbvCI CC'TCA_GC
Cuts at: 2952 2952
Size: 6503
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 160 194 823 857 90
Size: 34 36 34 629 34 5736
Fragments arranged by size:
5736 629 36 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1948 4401 5162 6503 1948
Size: 2453 761 1341 1948
Fragments arranged by size:
2453 1948 1341 761
BclI T'GATC_A
Cuts at: 1574 2099 4023 1574
Size: 525 1924 4054
Fragments arranged by size:
4054 1924 525
BfrBI ATG'CAT
Cuts at: 2965 2965
Size: 6503
BglII A'GATC_T
Cuts at: 1473 4018 6055 1473
Size: 2545 2037 1921
Fragments arranged by size:
2545 2037 1921
BlpI GC'TnA_GC
Cuts at: 3615 3615
Size: 6503
Bme1580I G_kGCm'C
Cuts at: 214 3207 4128 4221 5277 214
Size: 2993 921 93 1056 1440
Fragments arranged by size:
2993 1440 1056 921 93
BmgBI CAC'GTC
Cuts at: 300 477 300
Size: 177 6326
BmrI ACTGGGnnnn_n'
Cuts at: 200 529 1755 1877 2114 2359 3194 3715 3796
Size: 329 1226 122 237 245 835 521 81
Cuts at: 3796 4131 6275 200
Size: 335 2144 428
Fragments arranged by size:
2144 1226 835 521 428 335 329 245
237 122 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1685 4870 1685
Size: 3185 3318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 2832 110
Size: 2722 3781
Bpu10I CC'TnA_GC
Cuts at: 2952 2952
Size: 6503
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2259 3679 4558 5050 5348 2259
Size: 1420 879 492 298 3414
Fragments arranged by size:
3414 1420 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2774 2848 3006 6207 2774
Size: 74 158 3201 3070
Fragments arranged by size:
3201 3070 158 74
BsaAI yAC'GTr
Cuts at: 1614 3856 1614
Size: 2242 4261
BsaBI GATnn'nnATC
Cuts at: 6 4017 6054 6
Size: 4011 2037 455
Fragments arranged by size:
4011 2037 455
BsaHI Gr'CG_yC
Cuts at: 148 240 3934 4182 4884 6380 148
Size: 92 3694 248 702 1496 271
Fragments arranged by size:
3694 1496 702 271 248 92
BsaWI w'CCGG_w
Cuts at: 3261 3692 4213 4891 5165 5312 5723 3261
Size: 431 521 678 274 147 411 4041
Fragments arranged by size:
4041 678 521 431 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 649 679 2983 3013 649
Size: 30 2304 30 4139
Fragments arranged by size:
4139 2304 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 150 156 286 295 298 301 1059 2062 2940
Size: 6 130 9 3 3 758 1003 878
Cuts at: 2940 150
Size: 3713
Fragments arranged by size:
3713 1003 878 758 130 9 6 3
3
BseYI C'CCAG_C
Cuts at: 1252 2530 3928 4686 5263 5759 1252
Size: 1278 1398 758 577 496 1996
Fragments arranged by size:
1996 1398 1278 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 164
Size: 6503
BsiEI CG_ry'CG
Cuts at: 104 4045 4091 5299 104
Size: 3941 46 1208 1308
Fragments arranged by size:
3941 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 142 3517 4295 4485 5277 142
Size: 3375 778 190 792 1368
Fragments arranged by size:
3375 1368 792 778 190
BsmI GAATG_Cn'
Cuts at: 2726 2726
Size: 6503
BsmBI CGTCTCn'nnnn_
Cuts at: 483 3420 483
Size: 2937 3566
Bsp1286I G_dGCh'C
Cuts at: 142 214 1447 3207 3517 4128 4221 4295 4485
Size: 72 1233 1760 310 611 93 74 190
Cuts at: 4485 4547 5277 142
Size: 62 730 1368
Fragments arranged by size:
1760 1368 1233 730 611 310 190 93
74 72 62
BspEI T'CCGG_A
Cuts at: 3261 5723 3261
Size: 2462 4041
BspHI T'CATG_A
Cuts at: 5679 5679
Size: 6503
BspMI ACCTGCnnnn'nnnn_
Cuts at: 192 340 1794 2296 3553 3767 4069 4450 192
Size: 148 1454 502 1257 214 302 381 2245
Fragments arranged by size:
2245 1454 1257 502 381 302 214 148
BsrBI CCG'CTC
Cuts at: 50 4795 4849 6333 6498 50
Size: 4745 54 1484 165 55
Fragments arranged by size:
4745 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 971 1646 1763 2732 4415 971
Size: 675 117 969 1683 3059
Fragments arranged by size:
3059 1683 969 675 117
BsrFI r'CCGG_y
Cuts at: 4501 4682 4891 4501
Size: 181 209 6113
Fragments arranged by size:
6113 209 181
BssSI C'ACGA_G
Cuts at: 207 2035 4774 5132 207
Size: 1828 2739 358 1578
Fragments arranged by size:
2739 1828 1578 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2580 2962 3880 2580
Size: 382 918 5203
Fragments arranged by size:
5203 918 382
BstBI TT'CG_AA
Cuts at: 4864 4864
Size: 6503
BstXI CCAn_nnnn'nTGG
Cuts at: 2514 2856 2514
Size: 342 6161
BstYI r'GATC_y
Cuts at: 1 662 1473 2360 2494 2593 2659 3528 3995
Size: 661 811 887 134 99 66 869 467
Cuts at: 3995 4018 4353 4599 5611 5697 5709 6055 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 887 869 811 661 467 449 346
335 246 134 99 86 66 23 12
BstZ17I GTA'TAC
Cuts at: 824 824
Size: 6503
Bsu36I CC'TnA_GG
Cuts at: 2539 2539
Size: 6503
BtgI C'CryG_G
Cuts at: 389 733 795 1700 3083 389
Size: 344 62 905 1383 3809
Fragments arranged by size:
3809 1383 905 344 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2916 2975 4453 4634 4797 2916
Size: 59 1478 181 163 4622
Fragments arranged by size:
4622 1478 181 163 59
BtsI GCAGTG_nn'
Cuts at: 3120 3746 3120
Size: 626 5877
DraI TTT'AAA
Cuts at: 2555 3502 2555
Size: 947 5556
DraIII CAC_nnn'GTG
Cuts at: 1453 1453
Size: 6503
DrdI GACnn_nn'nnGTC
Cuts at: 4209 5067 4209
Size: 858 5645
EaeI y'GGCC_r
Cuts at: 457 1031 2391 2504 2691 4088 4262 4653 4680
Size: 574 1360 113 187 1397 174 391 27
Cuts at: 4680 6025 457
Size: 1345 935
Fragments arranged by size:
1397 1360 1345 935 574 391 187 174
113 27
EagI C'GGCC_G
Cuts at: 4088 4088
Size: 6503
EarI CTCTTCn'nnn_
Cuts at: 272 1096 1106 1866 2141 2146 2406 4526 4736
Size: 824 10 760 275 5 260 2120 210
Cuts at: 4736 272
Size: 2039
Fragments arranged by size:
2120 2039 824 760 275 260 210 10
5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2075 4711 5021 5167 6313 2075
Size: 2636 310 146 1146 2265
Fragments arranged by size:
2636 2265 1146 310 146
EcoICRI GAG'CTC
Cuts at: 140 3515 140
Size: 3375 3128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 1130 1370 2832 4328 4760 5507 5775 110
Size: 1020 240 1462 1496 432 747 268 838
Fragments arranged by size:
1496 1462 1020 838 747 432 268 240
EcoNI CCTnn'n_nnAGG
Cuts at: 231 3220 231
Size: 2989 3514
EcoO109I rG'GnC_Cy
Cuts at: 224 309 919 2568 3642 224
Size: 85 610 1649 1074 3085
Fragments arranged by size:
3085 1649 1074 610 85
EcoRI G'AATT_C
Cuts at: 2279 3507 2279
Size: 1228 5275
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3348 4522 3348
Size: 1174 5329
FspI TGC'GCA
Cuts at: 4284 4284
Size: 6503
HaeII r_GCGC'y
Cuts at: 151 243 3937 4185 5207 6186 151
Size: 92 3694 248 1022 979 468
Fragments arranged by size:
3694 1022 979 468 248 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1054 1086 1458 1490 1591 1623 1689 1721 1735
Size: 32 372 32 101 32 66 32 14
Cuts at: 1735 1767 2018 2050 2054 2086 2644 2676 2983
Size: 32 251 32 4 32 558 32 307
Cuts at: 2983 3015 3180 3212 3419 3451 4345 4375 4377
Size: 32 165 32 207 32 894 30 2
Cuts at: 4377 4407 1054
Size: 30 3150
Fragments arranged by size:
3150 894 558 372 307 251 207 165
101 66 32 32 32 32 32 32
32 32 32 32 32 30 30 14
4 2
HincII GTy'rAC
Cuts at: 3542 3542
Size: 6503
HindIII A'AGCT_T
Cuts at: 1244 1244
Size: 6503
KasI G'GCGC_C
Cuts at: 147 239 3933 4181 147
Size: 92 3694 248 2469
Fragments arranged by size:
3694 2469 248 92
KpnI G_GTAC'C
Cuts at: 3523 3523
Size: 6503
MfeI C'AATT_G
Cuts at: 839 839
Size: 6503
MluI A'CGCG_T
Cuts at: 5936 5936
Size: 6503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 506 2697 5174 5358 506
Size: 2191 2477 184 1651
Fragments arranged by size:
2477 2191 1651 184
MscI TGG'CCA
Cuts at: 459 1033 2393 2693 4264 459
Size: 574 1360 300 1571 2698
Fragments arranged by size:
2698 1571 1360 574 300
MslI CAynn'nnrTG
Cuts at: 574 1936 2512 2983 3217 4492 4619 574
Size: 1362 576 471 234 1275 127 2458
Fragments arranged by size:
2458 1362 1275 576 471 234 127
MspA1I CmG'CkG
Cuts at: 178 1851 2053 3111 3614 3928 4288 5301 5546
Size: 1673 202 1058 503 314 360 1013 245
Cuts at: 5546 178
Size: 1135
Fragments arranged by size:
1673 1135 1058 1013 503 360 314 245
202
NaeI GCC'GGC
Cuts at: 4684 4684
Size: 6503
NarI GG'CG_CC
Cuts at: 148 240 3934 4182 148
Size: 92 3694 248 2469
Fragments arranged by size:
3694 2469 248 92
NcoI C'CATG_G
Cuts at: 389 733 3083 389
Size: 344 2350 3809
Fragments arranged by size:
3809 2350 344
NdeI CA'TA_TG
Cuts at: 2223 2223
Size: 6503
NgoMIV G'CCGG_C
Cuts at: 4682 4682
Size: 6503
NsiI A_TGCA'T
Cuts at: 2967 2967
Size: 6503
NspI r_CATG'y
Cuts at: 1373 3556 4056 4963 1373
Size: 2183 500 907 2913
Fragments arranged by size:
2913 2183 907 500
PciI A'CATG_T
Cuts at: 4959 4959
Size: 6503
PflMI CCAn_nnn'nTGG
Cuts at: 553 2912 553
Size: 2359 4144
PfoI T'CCnGG_A
Cuts at: 407 407
Size: 6503
PmeI GTTT'AAAC
Cuts at: 3502 3502
Size: 6503
PmlI CAC'GTG
Cuts at: 1614 1614
Size: 6503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5642 5674 5642
Size: 32 6471
PpuMI rG'GwC_Cy
Cuts at: 224 309 224
Size: 85 6418
PshAI GACnn'nnGTC
Cuts at: 470 470
Size: 6503
PstI C_TGCA'G
Cuts at: 472 1186 3550 4235 472
Size: 714 2364 685 2740
Fragments arranged by size:
2740 2364 714 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6503
PvuII CAG'CTG
Cuts at: 178 1851 3111 3928 4288 178
Size: 1673 1260 817 360 2393
Fragments arranged by size:
2393 1673 1260 817 360
RsrII CG'GwC_CG
Cuts at: 4698 4698
Size: 6503
SacI G_AGCT'C
Cuts at: 142 3517 142
Size: 3375 3128
SalI G'TCGA_C
Cuts at: 3540 3540
Size: 6503
SapI GCTCTTCn'nnn_
Cuts at: 1106 4526 4736 1106
Size: 3420 210 2873
Fragments arranged by size:
3420 2873 210
SbfI CC_TGCA'GG
Cuts at: 3550 3550
Size: 6503
ScaI AGT'ACT
Cuts at: 1595 1828 6020 1595
Size: 233 4192 2078
Fragments arranged by size:
4192 2078 233
SexAI A'CCwGG_T
Cuts at: 608 608
Size: 6503
SfcI C'TryA_G
Cuts at: 33 468 923 1182 1965 2325 2937 3546 4231
Size: 435 455 259 783 360 612 609 685
Cuts at: 4231 5224 5415 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 783 685 612 609 455 435
360 259 191
SfoI GGC'GCC
Cuts at: 149 241 3935 4183 149
Size: 92 3694 248 2469
Fragments arranged by size:
3694 2469 248 92
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6503
SmaI CCC'GGG
Cuts at: 222 246 2192 3525 222
Size: 24 1946 1333 3200
Fragments arranged by size:
3200 1946 1333 24
SmlI C'TyrA_G
Cuts at: 1903 2274 3658 4573 5065 5327 1903
Size: 371 1384 915 492 262 3079
Fragments arranged by size:
3079 1384 915 492 371 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6503
SphI G_CATG'C
Cuts at: 3556 4056 3556
Size: 500 6003
StuI AGG'CCT
Cuts at: 1571 1571
Size: 6503
StyI C'CwwG_G
Cuts at: 379 389 733 1054 1393 3083 3637 3991 379
Size: 10 344 321 339 1690 554 354 2891
Fragments arranged by size:
2891 1690 554 354 344 339 321 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 545 865 4086 4219 4678 4887 545
Size: 320 3221 133 459 209 2161
Fragments arranged by size:
3221 2161 459 320 209 133
TatI w'GTAC_w
Cuts at: 516 1186 1593 1826 6018 516
Size: 670 407 233 4192 1001
Fragments arranged by size:
4192 1001 670 407 233
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 654 686 6020 6052 654
Size: 32 5334 32 1105
Fragments arranged by size:
5334 1105 32 32
Tth111I GACn'n_nGTC
Cuts at: 3166 4300 3166
Size: 1134 5369
XbaI T'CTAG_A
Cuts at: 3534 3534
Size: 6503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 554 573 2170 3998 554
Size: 19 1597 1828 3059
Fragments arranged by size:
3059 1828 1597 19
XmaI C'CCGG_G
Cuts at: 220 244 2190 3523 220
Size: 24 1946 1333 3200
Fragments arranged by size:
3200 1946 1333 24
XmnI GAAnn'nnTTC
Cuts at: 3331 3331
Size: 6503
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AloI ApaI AscI BaeI BglI BmtI
BsiWI BsrGI BssHII BstEII ClaI CspCI EcoRV FseI
FspAI HpaI NheI NotI NruI PacI PasI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI