Restriction map for
pF1KE3543
(Circular) (Six-base) MAPSORT of: pF1KE3543.seq Check: 2815 from: 1 to: 6761
pF1KE3543 6761 bp
With 182 enzymes: SgfI *
July 1, 2016 17:02 ..
AccI GT'mk_AC
Cuts at: 483 2566 3799 483
Size: 2083 1233 3445
Fragments arranged by size:
3445 2083 1233
Acc65I G'GTAC_C
Cuts at: 3777 3777
Size: 6761
AclI AA'CG_TT
Cuts at: 6601 6601
Size: 6761
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 929 1131 1459 1720 2423 2773 2969 4586 5018
Size: 202 328 261 703 350 196 1617 432
Cuts at: 5018 5765 6033 929
Size: 747 268 1657
Fragments arranged by size:
1657 1617 747 703 432 350 328 268
261 202 196
AflIII A'CryG_T
Cuts at: 343 405 4113 5217 6194 343
Size: 62 3708 1104 977 910
Fragments arranged by size:
3708 1104 977 910 62
AgeI A'CCGG_T
Cuts at: 5149 5149
Size: 6761
AhdI GACnn_n'nnGTC
Cuts at: 2198 2850 2198
Size: 652 6109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1214 1214
Size: 6761
AlwNI CAG_nnn'CTG
Cuts at: 119 826 1179 1311 1323 1437 1569 1786 2392
Size: 707 353 132 12 114 132 217 606
Cuts at: 2392 2410 3502 4066 5633 119
Size: 18 1092 564 1567 1247
Fragments arranged by size:
1567 1247 1092 707 606 564 353 217
132 132 114 18 12
ApaI G_GGCC'C
Cuts at: 232 249 2318 2995 232
Size: 17 2069 677 3998
Fragments arranged by size:
3998 2069 677 17
ApaLI G'TGCA_C
Cuts at: 5531 5531
Size: 6761
ApoI r'AATT_y
Cuts at: 714 2607 3765 6114 6370 714
Size: 1893 1158 2349 256 1105
Fragments arranged by size:
2349 1893 1158 1105 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6761
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6761
AvaI C'yCGr_G
Cuts at: 223 242 3166 3781 223
Size: 19 2924 615 3203
Fragments arranged by size:
3203 2924 615 19
BamHI G'GATC_C
Cuts at: 3786 3786
Size: 6761
BanI G'GyrC_C
Cuts at: 1167 2101 2547 3777 4191 4439 4474 1167
Size: 934 446 1230 414 248 35 3454
Fragments arranged by size:
3454 1230 934 446 414 248 35
BanII G_rGCy'C
Cuts at: 232 249 1377 1594 1835 1852 2318 2625 2995
Size: 17 1128 217 241 17 466 307 370
Cuts at: 2995 3775 4805 232
Size: 780 1030 2188
Fragments arranged by size:
2188 1128 1030 780 466 370 307 241
217 17 17
BbeI G_GCGC'C
Cuts at: 2551 4195 4443 2551
Size: 1644 248 4869
Fragments arranged by size:
4869 1644 248
BbvCI CC'TCA_GC
Cuts at: 1727 3292 1727
Size: 1565 5196
BciVI GTATCCnnnnn_n'
Cuts at: 4659 5420 6761 4659
Size: 761 1341 4659
Fragments arranged by size:
4659 1341 761
BclI T'GATC_A
Cuts at: 2298 2769 2792 4281 2298
Size: 471 23 1489 4778
Fragments arranged by size:
4778 1489 471 23
BfrBI ATG'CAT
Cuts at: 1632 1632
Size: 6761
BglI GCCn_nnn'nGGC
Cuts at: 1598 2110 2866 3041 1598
Size: 512 756 175 5318
Fragments arranged by size:
5318 756 512 175
BglII A'GATC_T
Cuts at: 2711 3545 4276 6313 2711
Size: 834 731 2037 3159
Fragments arranged by size:
3159 2037 834 731
BlpI GC'TnA_GC
Cuts at: 1001 1996 3112 3873 1001
Size: 995 1116 761 3889
Fragments arranged by size:
3889 1116 995 761
Bme1580I G_kGCm'C
Cuts at: 232 249 1172 2106 2219 2318 2941 2995 4386
Size: 17 923 934 113 99 623 54 1391
Cuts at: 4386 4479 5535 232
Size: 93 1056 1458
Fragments arranged by size:
1458 1391 1056 934 923 623 113 99
93 54 17
BmgBI CAC'GTC
Cuts at: 2564 2564
Size: 6761
BmrI ACTGGGnnnn_n'
Cuts at: 735 1081 1930 2420 3973 4054 4389 6533 735
Size: 346 849 490 1553 81 335 2144 963
Fragments arranged by size:
2144 1553 963 849 490 346 335 81
BmtI G_CTAG'C
Cuts at: 886 886
Size: 6761
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2399 5128 2399
Size: 2729 4032
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 823 977 1028 1666 1669 2044 2369 2406
Size: 578 154 51 638 3 375 325 37
Cuts at: 2406 2500 2527 2719 2802 2899 245
Size: 94 27 192 83 97 4107
Fragments arranged by size:
4107 638 578 375 325 192 154 97
94 83 51 37 27 3
Bpu10I CC'TnA_GC
Cuts at: 1727 3232 3292 1727
Size: 1505 60 5196
Fragments arranged by size:
5196 1505 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 2692 3937 4816 5308 5606 310
Size: 2382 1245 879 492 298 1465
Fragments arranged by size:
2382 1465 1245 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1190 1804 6465 1190
Size: 614 4661 1486
Fragments arranged by size:
4661 1486 614
BsaAI yAC'GTr
Cuts at: 4114 4114
Size: 6761
BsaBI GATnn'nnATC
Cuts at: 6 4275 6312 6
Size: 4269 2037 455
Fragments arranged by size:
4269 2037 455
BsaHI Gr'CG_yC
Cuts at: 1903 2548 4192 4440 5142 6638 1903
Size: 645 1644 248 702 1496 2026
Fragments arranged by size:
2026 1644 1496 702 645 248
BsaWI w'CCGG_w
Cuts at: 3377 3950 4471 5149 5423 5570 5981 3377
Size: 573 521 678 274 147 411 4157
Fragments arranged by size:
4157 678 573 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1481 1511 1707 1737 2017 2047 2436 2466 3119
Size: 30 196 30 280 30 389 30 653
Cuts at: 3119 3149 3547 3577 3607 1481
Size: 30 398 30 30 4635
Fragments arranged by size:
4635 653 398 389 280 196 30 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1047 1715 1822 1950 2524 2715 3439 3613 1047
Size: 668 107 128 574 191 724 174 4195
Fragments arranged by size:
4195 724 668 574 191 174 128 107
BseYI C'CCAG_C
Cuts at: 1123 1134 1172 1179 1274 1304 1311 1331 1382
Size: 11 38 7 95 30 7 20 51
Cuts at: 1382 1562 1696 1836 2185 2218 2311 2317 2911
Size: 180 134 140 349 33 93 6 594
Cuts at: 2911 2997 3161 3402 3569 4186 4944 5521 6017
Size: 86 164 241 167 617 758 577 496
Cuts at: 6017 1123
Size: 1867
Fragments arranged by size:
1867 758 617 594 577 496 349 241
180 167 164 140 134 95 93 86
51 38 33 30 20 11 7 7
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 204 420 730 144
Size: 60 216 310 6175
Fragments arranged by size:
6175 310 216 60
BsiEI CG_ry'CG
Cuts at: 104 4303 4349 5557 104
Size: 4199 46 1208 1308
Fragments arranged by size:
4199 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1057 1198 1300 2136 3775 4553 4743 5535 1057
Size: 141 102 836 1639 778 190 792 2283
Fragments arranged by size:
2283 1639 836 792 778 190 141 102
BsmI GAATG_Cn'
Cuts at: 2490 3195 3218 2490
Size: 705 23 6033
Fragments arranged by size:
6033 705 23
Bsp1286I G_dGCh'C
Cuts at: 232 249 1057 1172 1198 1300 1377 1594 1835
Size: 17 808 115 26 102 77 217 241
Cuts at: 1835 1852 2106 2136 2219 2318 2625 2941 2995
Size: 17 254 30 83 99 307 316 54
Cuts at: 2995 3775 4386 4479 4553 4743 4805 5535 232
Size: 780 611 93 74 190 62 730 1458
Fragments arranged by size:
1458 808 780 730 611 316 307 254
241 217 190 115 102 99 93 83
77 74 62 54 30 26 17 17
BspEI T'CCGG_A
Cuts at: 3377 5981 3377
Size: 2604 4157
BspHI T'CATG_A
Cuts at: 5937 5937
Size: 6761
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3811 4025 4327 4708 3811
Size: 214 302 381 5864
Fragments arranged by size:
5864 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5053 5107 6591 6756 50
Size: 5003 54 1484 165 55
Fragments arranged by size:
5003 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4673 4673
Size: 6761
BsrFI r'CCGG_y
Cuts at: 1857 4759 4940 5149 1857
Size: 2902 181 209 3469
Fragments arranged by size:
3469 2902 209 181
BssSI C'ACGA_G
Cuts at: 1585 5032 5390 1585
Size: 3447 358 2956
Fragments arranged by size:
3447 2956 358
BstAPI GCAn_nnn'nTGC
Cuts at: 167 191 798 4138 167
Size: 24 607 3340 2790
Fragments arranged by size:
3340 2790 607 24
BstBI TT'CG_AA
Cuts at: 5122 5122
Size: 6761
BstXI CCAn_nnnn'nTGG
Cuts at: 1906 2022 1906
Size: 116 6645
BstYI r'GATC_y
Cuts at: 1 256 822 2072 2711 3545 3786 4253 4276
Size: 255 566 1250 639 834 241 467 23
Cuts at: 4276 4611 4857 5869 5955 5967 6313 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1250 1012 834 639 566 467 449 346
335 255 246 241 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2273 2306 2727 2273
Size: 33 421 6307
Fragments arranged by size:
6307 421 33
BtgI C'CryG_G
Cuts at: 107 1283 3359 3643 107
Size: 1176 2076 284 3225
Fragments arranged by size:
3225 2076 1176 284
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4711 4892 5055 4711
Size: 181 163 6417
Fragments arranged by size:
6417 181 163
BtsI GCAGTG_nn'
Cuts at: 1055 1077 1198 4004 1055
Size: 22 121 2806 3812
Fragments arranged by size:
3812 2806 121 22
DraI TTT'AAA
Cuts at: 3760 3760
Size: 6761
DrdI GACnn_nn'nnGTC
Cuts at: 4467 5325 4467
Size: 858 5903
EaeI y'GGCC_r
Cuts at: 110 566 1422 2430 2920 3504 3734 4346 4520
Size: 456 856 1008 490 584 230 612 174
Cuts at: 4520 4911 4938 6283 110
Size: 391 27 1345 588
Fragments arranged by size:
1345 1008 856 612 588 584 490 456
391 230 174 27
EagI C'GGCC_G
Cuts at: 4346 4346
Size: 6761
EarI CTCTTCn'nnn_
Cuts at: 133 1556 4784 4994 133
Size: 1423 3228 210 1900
Fragments arranged by size:
3228 1900 1423 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3059 4969 5279 5425 6571 3059
Size: 1910 310 146 1146 3249
Fragments arranged by size:
3249 1910 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3773 3773
Size: 6761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 823 929 977 1028 1131 1459 1666 1669
Size: 578 106 48 51 103 328 207 3
Cuts at: 1669 1720 2044 2369 2406 2423 2500 2527 2719
Size: 51 324 325 37 17 77 27 192
Cuts at: 2719 2773 2802 2899 2969 4586 5018 5765 6033
Size: 54 29 97 70 1617 432 747 268
Cuts at: 6033 245
Size: 973
Fragments arranged by size:
1617 973 747 578 432 328 325 324
268 207 192 106 103 97 77 70
54 51 51 48 37 29 27 17
3
EcoO109I rG'GnC_Cy
Cuts at: 228 1113 2057 2868 2991 2992 3399 3900 228
Size: 885 944 811 123 1 407 501 3089
Fragments arranged by size:
3089 944 885 811 501 407 123 1
EcoRI G'AATT_C
Cuts at: 3765 3765
Size: 6761
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1030 4780 1030
Size: 3750 3011
FspI TGC'GCA
Cuts at: 4542 4542
Size: 6761
HaeII r_GCGC'y
Cuts at: 2551 4195 4443 5465 6444 2551
Size: 1644 248 1022 979 2868
Fragments arranged by size:
2868 1644 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 814 846 1285 1317 1707 1739 2023 2055 2184
Size: 32 439 32 390 32 284 32 129
Cuts at: 2184 2216 2436 2468 2696 2728 2836 2868 2926
Size: 32 220 32 228 32 108 32 58
Cuts at: 2926 2958 3418 3450 3577 3589 3609 3621 4603
Size: 32 460 32 127 12 20 12 982
Cuts at: 4603 4633 4635 4665 814
Size: 30 2 30 2910
Fragments arranged by size:
2910 982 460 439 390 284 228 220
129 127 108 58 32 32 32 32
32 32 32 32 32 32 30 30
20 12 12 2
HincII GTy'rAC
Cuts at: 3800 3800
Size: 6761
KasI G'GCGC_C
Cuts at: 2547 4191 4439 2547
Size: 1644 248 4869
Fragments arranged by size:
4869 1644 248
KpnI G_GTAC'C
Cuts at: 3781 3781
Size: 6761
MluI A'CGCG_T
Cuts at: 6194 6194
Size: 6761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2030 2039 3696 5432 5616 2030
Size: 9 1657 1736 184 3175
Fragments arranged by size:
3175 1736 1657 184 9
MscI TGG'CCA
Cuts at: 112 568 1424 3736 4522 112
Size: 456 856 2312 786 2351
Fragments arranged by size:
2351 2312 856 786 456
MslI CAynn'nnrTG
Cuts at: 1065 4750 4877 1065
Size: 3685 127 2949
Fragments arranged by size:
3685 2949 127
MspA1I CmG'CkG
Cuts at: 1179 1308 1323 2098 2919 3068 3509 3872 4186
Size: 129 15 775 821 149 441 363 314
Cuts at: 4186 4546 5559 5804 1179
Size: 360 1013 245 2136
Fragments arranged by size:
2136 1013 821 775 441 363 360 314
245 149 129 15
NaeI GCC'GGC
Cuts at: 1859 4942 1859
Size: 3083 3678
NarI GG'CG_CC
Cuts at: 2548 4192 4440 2548
Size: 1644 248 4869
Fragments arranged by size:
4869 1644 248
NcoI C'CATG_G
Cuts at: 107 3359 107
Size: 3252 3509
NdeI CA'TA_TG
Cuts at: 3695 3695
Size: 6761
NgoMIV G'CCGG_C
Cuts at: 1857 4940 1857
Size: 3083 3678
NheI G'CTAG_C
Cuts at: 882 882
Size: 6761
NsiI A_TGCA'T
Cuts at: 1634 1634
Size: 6761
NspI r_CATG'y
Cuts at: 347 409 709 2400 2826 3240 3660 3814 4314
Size: 62 300 1691 426 414 420 154 500
Cuts at: 4314 5221 347
Size: 907 1887
Fragments arranged by size:
1887 1691 907 500 426 420 414 300
154 62
PasI CC'CwG_GG
Cuts at: 1211 3121 3633 1211
Size: 1910 512 4339
Fragments arranged by size:
4339 1910 512
PciI A'CATG_T
Cuts at: 343 405 5217 343
Size: 62 4812 1887
Fragments arranged by size:
4812 1887 62
PflMI CCAn_nnn'nTGG
Cuts at: 1179 1311 1569 2410 1179
Size: 132 258 841 5530
Fragments arranged by size:
5530 841 258 132
PfoI T'CCnGG_A
Cuts at: 261 279 261
Size: 18 6743
PmeI GTTT'AAAC
Cuts at: 3760 3760
Size: 6761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2945 2977 3606 3638 5900 5932 2945
Size: 32 629 32 2262 32 3774
Fragments arranged by size:
3774 2262 629 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1113 3399 1113
Size: 2286 4475
PshAI GACnn'nnGTC
Cuts at: 921 921
Size: 6761
PspOMI G'GGCC_C
Cuts at: 228 245 2314 2991 228
Size: 17 2069 677 3998
Fragments arranged by size:
3998 2069 677 17
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 653 685 653
Size: 32 6729
PstI C_TGCA'G
Cuts at: 739 1193 1234 1259 1820 2375 2579 3808 4493
Size: 454 41 25 561 555 204 1229 685
Cuts at: 4493 739
Size: 3007
Fragments arranged by size:
3007 1229 685 561 555 454 204 41
25
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6761
PvuII CAG'CTG
Cuts at: 1179 1323 2098 4186 4546 1179
Size: 144 775 2088 360 3394
Fragments arranged by size:
3394 2088 775 360 144
RsrII CG'GwC_CG
Cuts at: 4956 4956
Size: 6761
SacI G_AGCT'C
Cuts at: 3775 3775
Size: 6761
SalI G'TCGA_C
Cuts at: 3798 3798
Size: 6761
SapI GCTCTTCn'nnn_
Cuts at: 4784 4994 4784
Size: 210 6551
SbfI CC_TGCA'GG
Cuts at: 1820 3808 1820
Size: 1988 4773
ScaI AGT'ACT
Cuts at: 323 6278 323
Size: 5955 806
SexAI A'CCwGG_T
Cuts at: 2044 2044
Size: 6761
SfcI C'TryA_G
Cuts at: 33 484 735 1189 1230 1255 1816 1823 2206
Size: 451 251 454 41 25 561 7 383
Cuts at: 2206 2371 2567 2575 3804 4489 5482 5673 33
Size: 165 196 8 1229 685 993 191 1121
Fragments arranged by size:
1229 1121 993 685 561 454 451 383
251 196 191 165 41 25 8 7
SfiI GGCCn_nnn'nGGCC
Cuts at: 2866 2866
Size: 6761
SfoI GGC'GCC
Cuts at: 2549 4193 4441 2549
Size: 1644 248 4869
Fragments arranged by size:
4869 1644 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6761
SmaI CCC'GGG
Cuts at: 225 3783 225
Size: 3558 3203
SmlI C'TyrA_G
Cuts at: 325 2707 3916 4831 5323 5585 325
Size: 2382 1209 915 492 262 1501
Fragments arranged by size:
2382 1501 1209 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6761
SphI G_CATG'C
Cuts at: 3814 4314 3814
Size: 500 6261
StyI C'CwwG_G
Cuts at: 107 835 3359 3574 3895 4249 107
Size: 728 2524 215 321 354 2619
Fragments arranged by size:
2619 2524 728 354 321 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1137 1148 1559 2353 2878 3123 4344 4477 4936
Size: 11 411 794 525 245 1221 133 459
Cuts at: 4936 5145 1137
Size: 209 2753
Fragments arranged by size:
2753 1221 794 525 459 411 245 209
133 11
TatI w'GTAC_w
Cuts at: 321 6276 321
Size: 5955 806
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1019 1051 1391 1423 1481 1513 3119 3151 6278
Size: 32 340 32 58 32 1606 32 3127
Cuts at: 6278 6310 1019
Size: 32 1470
Fragments arranged by size:
3127 1606 1470 340 58 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4558 4558
Size: 6761
XbaI T'CTAG_A
Cuts at: 3792 3792
Size: 6761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1417 3128 4256 1417
Size: 1711 1128 3922
Fragments arranged by size:
3922 1711 1128
XmaI C'CCGG_G
Cuts at: 223 3781 223
Size: 3558 3203
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BaeI BbsI BcgI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NotI NruI PacI PmlI PsiI
PspXI SacII SanDI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI