Restriction map for
pF1KE3561
(Circular) (Six-base) MAPSORT of: pF1KE3561.seq Check: 7778 from: 1 to: 5891
pF1KE3561 5891 bp
With 182 enzymes: SgfI *
August 31, 2012 11:48 ..
AccI GT'mk_AC
Cuts at: 2929 2929
Size: 5891
Acc65I G'GTAC_C
Cuts at: 2907 2907
Size: 5891
AclI AA'CG_TT
Cuts at: 5731 5731
Size: 5891
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 824 913 2221 2785 3716 4148 4895 5163 824
Size: 89 1308 564 931 432 747 268 1552
Fragments arranged by size:
1552 1308 931 747 564 432 268 89
AflII C'TTAA_G
Cuts at: 1651 1651
Size: 5891
AflIII A'CryG_T
Cuts at: 815 2080 3243 4347 5324 815
Size: 1265 1163 1104 977 1382
Fragments arranged by size:
1382 1265 1163 1104 977
AgeI A'CCGG_T
Cuts at: 4279 4279
Size: 5891
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1208 2841 1208
Size: 1633 4258
AlwNI CAG_nnn'CTG
Cuts at: 3196 4763 3196
Size: 1567 4324
ApaI G_GGCC'C
Cuts at: 1824 1824
Size: 5891
ApaLI G'TGCA_C
Cuts at: 4661 4661
Size: 5891
ApoI r'AATT_y
Cuts at: 463 585 644 1485 1658 2156 2194 2452 2895
Size: 122 59 841 173 498 38 258 443
Cuts at: 2895 5244 5500 463
Size: 2349 256 854
Fragments arranged by size:
2349 854 841 498 443 258 256 173
122 59 38
AseI AT'TA_AT
Cuts at: 20 2563 20
Size: 2543 3348
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5891
AvaI C'yCGr_G
Cuts at: 2911 2911
Size: 5891
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2831 2864 2831
Size: 33 5858
BamHI G'GATC_C
Cuts at: 2347 2916 2347
Size: 569 5322
BanI G'GyrC_C
Cuts at: 2365 2907 3321 3569 3604 2365
Size: 542 414 248 35 4652
Fragments arranged by size:
4652 542 414 248 35
BanII G_rGCy'C
Cuts at: 1824 2905 3935 1824
Size: 1081 1030 3780
Fragments arranged by size:
3780 1081 1030
BbeI G_GCGC'C
Cuts at: 3325 3573 3325
Size: 248 5643
BbsI GAAGACnn'nnnn_
Cuts at: 269 1599 269
Size: 1330 4561
BciVI GTATCCnnnnn_n'
Cuts at: 3789 4550 5891 3789
Size: 761 1341 3789
Fragments arranged by size:
3789 1341 761
BclI T'GATC_A
Cuts at: 356 3411 356
Size: 3055 2836
BfrBI ATG'CAT
Cuts at: 1185 1232 1185
Size: 47 5844
BglI GCCn_nnn'nGGC
Cuts at: 1267 2392 1267
Size: 1125 4766
BglII A'GATC_T
Cuts at: 2796 3406 5443 2796
Size: 610 2037 3244
Fragments arranged by size:
3244 2037 610
BlpI GC'TnA_GC
Cuts at: 1328 3003 1328
Size: 1675 4216
Bme1580I G_kGCm'C
Cuts at: 677 1824 3516 3609 4665 677
Size: 1147 1692 93 1056 1903
Fragments arranged by size:
1903 1692 1147 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 400 671 1142 1827 1924 3103 3184 3519 5663
Size: 271 471 685 97 1179 81 335 2144
Cuts at: 5663 400
Size: 628
Fragments arranged by size:
2144 1179 685 628 471 335 271 97
81
BmtI G_CTAG'C
Cuts at: 2167 2167
Size: 5891
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2135 4258 2135
Size: 2123 3768
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 459 558 2353 401
Size: 58 99 1795 3939
Fragments arranged by size:
3939 1795 99 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2641 3067 3946 4438 4736 2641
Size: 426 879 492 298 3796
Fragments arranged by size:
3796 879 492 426 298
BsaI GGTCTCn'nnnn_
Cuts at: 5595 5595
Size: 5891
BsaAI yAC'GTr
Cuts at: 3244 3244
Size: 5891
BsaBI GATnn'nnATC
Cuts at: 6 742 3405 5442 6
Size: 736 2663 2037 455
Fragments arranged by size:
2663 2037 736 455
BsaHI Gr'CG_yC
Cuts at: 3322 3570 4272 5768 3322
Size: 248 702 1496 3445
Fragments arranged by size:
3445 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3080 3601 4279 4553 4700 5111 3080
Size: 521 678 274 147 411 3860
Fragments arranged by size:
3860 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 481 511 481
Size: 30 5861
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2356 2356
Size: 5891
BseYI C'CCAG_C
Cuts at: 2373 2755 3316 4074 4651 5147 2373
Size: 382 561 758 577 496 3117
Fragments arranged by size:
3117 758 577 561 496 382
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1120 1120
Size: 5891
BsiEI CG_ry'CG
Cuts at: 104 3433 3479 4687 104
Size: 3329 46 1208 1308
Fragments arranged by size:
3329 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1541 1837 2905 3683 3873 4665 1541
Size: 296 1068 778 190 792 2767
Fragments arranged by size:
2767 1068 792 778 296 190
BsmI GAATG_Cn'
Cuts at: 112 1687 112
Size: 1575 4316
Bsp1286I G_dGCh'C
Cuts at: 677 1541 1824 1837 2905 3516 3609 3683 3873
Size: 864 283 13 1068 611 93 74 190
Cuts at: 3873 3935 4665 677
Size: 62 730 1903
Fragments arranged by size:
1903 1068 864 730 611 283 190 93
74 62 13
BspEI T'CCGG_A
Cuts at: 5111 5111
Size: 5891
BspHI T'CATG_A
Cuts at: 240 1298 5067 240
Size: 1058 3769 1064
Fragments arranged by size:
3769 1064 1058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 142 2941 3155 3457 3838 142
Size: 2799 214 302 381 2195
Fragments arranged by size:
2799 2195 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2732 4183 4237 5721 5886 50
Size: 2682 1451 54 1484 165 55
Fragments arranged by size:
2682 1484 1451 165 55 54
BsrDI GCAATG_nn'
Cuts at: 440 707 2381 2696 2742 3803 440
Size: 267 1674 315 46 1061 2528
Fragments arranged by size:
2528 1674 1061 315 267 46
BsrFI r'CCGG_y
Cuts at: 2538 3889 4070 4279 2538
Size: 1351 181 209 4150
Fragments arranged by size:
4150 1351 209 181
BsrGI T'GTAC_A
Cuts at: 415 415
Size: 5891
BssSI C'ACGA_G
Cuts at: 1403 2046 4162 4520 1403
Size: 643 2116 358 2774
Fragments arranged by size:
2774 2116 643 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1366 1841 2132 3268 1366
Size: 475 291 1136 3989
Fragments arranged by size:
3989 1136 475 291
BstBI TT'CG_AA
Cuts at: 4252 4252
Size: 5891
BstYI r'GATC_y
Cuts at: 1 1389 2347 2796 2916 3383 3406 3741 3987
Size: 1388 958 449 120 467 23 335 246
Cuts at: 3987 4999 5085 5097 5443 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1388 1012 958 467 449 449 346 335
246 120 86 23 12
BtgI C'CryG_G
Cuts at: 107 1435 107
Size: 1328 4563
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3841 4022 4185 3841
Size: 181 163 5547
Fragments arranged by size:
5547 181 163
BtsI GCAGTG_nn'
Cuts at: 1367 1788 3134 1367
Size: 421 1346 4124
Fragments arranged by size:
4124 1346 421
DraI TTT'AAA
Cuts at: 1517 2890 1517
Size: 1373 4518
DraIII CAC_nnn'GTG
Cuts at: 1841 1841
Size: 5891
DrdI GACnn_nn'nnGTC
Cuts at: 3597 4455 3597
Size: 858 5033
EaeI y'GGCC_r
Cuts at: 1886 3476 3650 4041 4068 5413 1886
Size: 1590 174 391 27 1345 2364
Fragments arranged by size:
2364 1590 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3476 3476
Size: 5891
EarI CTCTTCn'nnn_
Cuts at: 970 1089 3914 4124 970
Size: 119 2825 210 2737
Fragments arranged by size:
2825 2737 210 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4099 4409 4555 5701 4099
Size: 310 146 1146 4289
Fragments arranged by size:
4289 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2903 2903
Size: 5891
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 459 558 824 913 2221 2353 2785 3716
Size: 58 99 266 89 1308 132 432 931
Cuts at: 3716 4148 4895 5163 401
Size: 432 747 268 1129
Fragments arranged by size:
1308 1129 931 747 432 432 268 266
132 99 89 58
EcoO109I rG'GnC_Cy
Cuts at: 1821 3030 1821
Size: 1209 4682
EcoRI G'AATT_C
Cuts at: 1485 2895 1485
Size: 1410 4481
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 856 2667 3910 856
Size: 1811 1243 2837
Fragments arranged by size:
2837 1811 1243
FspI TGC'GCA
Cuts at: 1200 3672 1200
Size: 2472 3419
HaeII r_GCGC'y
Cuts at: 3325 3573 4595 5574 3325
Size: 248 1022 979 3642
Fragments arranged by size:
3642 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 269 301 1006 1038 1985 2017 2133 2165 2312
Size: 32 705 32 947 32 116 32 147
Cuts at: 2312 2332 2344 2364 3733 3763 3765 3795 269
Size: 20 12 20 1369 30 2 30 2365
Fragments arranged by size:
2365 1369 947 705 147 116 32 32
32 32 30 30 20 20 12 2
HincII GTy'rAC
Cuts at: 1025 1720 2930 1025
Size: 695 1210 3986
Fragments arranged by size:
3986 1210 695
KasI G'GCGC_C
Cuts at: 3321 3569 3321
Size: 248 5643
KpnI G_GTAC'C
Cuts at: 2911 2911
Size: 5891
MluI A'CGCG_T
Cuts at: 5324 5324
Size: 5891
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 709 4562 4746 709
Size: 3853 184 1854
Fragments arranged by size:
3853 1854 184
MscI TGG'CCA
Cuts at: 1888 3652 1888
Size: 1764 4127
MslI CAynn'nnrTG
Cuts at: 352 982 1175 1499 1576 2823 3880 4007 352
Size: 630 193 324 77 1247 1057 127 2236
Fragments arranged by size:
2236 1247 1057 630 324 193 127 77
MspA1I CmG'CkG
Cuts at: 3002 3316 3676 4689 4934 3002
Size: 314 360 1013 245 3959
Fragments arranged by size:
3959 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4072 4072
Size: 5891
NarI GG'CG_CC
Cuts at: 3322 3570 3322
Size: 248 5643
NcoI C'CATG_G
Cuts at: 107 1435 107
Size: 1328 4563
NdeI CA'TA_TG
Cuts at: 1203 2824 1203
Size: 1621 4270
NgoMIV G'CCGG_C
Cuts at: 4070 4070
Size: 5891
NheI G'CTAG_C
Cuts at: 2163 2163
Size: 5891
NsiI A_TGCA'T
Cuts at: 1187 1234 1187
Size: 47 5844
NspI r_CATG'y
Cuts at: 519 2084 2472 2944 3444 4351 519
Size: 1565 388 472 500 907 2059
Fragments arranged by size:
2059 1565 907 500 472 388
PciI A'CATG_T
Cuts at: 2080 4347 2080
Size: 2267 3624
PfoI T'CCnGG_A
Cuts at: 204 287 574 2330 204
Size: 83 287 1756 3765
Fragments arranged by size:
3765 1756 287 83
PmeI GTTT'AAAC
Cuts at: 2890 2890
Size: 5891
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5030 5062 5030
Size: 32 5859
PsiI TTA'TAA
Cuts at: 793 793
Size: 5891
PspOMI G'GGCC_C
Cuts at: 1820 1820
Size: 5891
PstI C_TGCA'G
Cuts at: 627 2938 3623 627
Size: 2311 685 2895
Fragments arranged by size:
2895 2311 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5891
PvuII CAG'CTG
Cuts at: 3316 3676 3316
Size: 360 5531
RsrII CG'GwC_CG
Cuts at: 4086 4086
Size: 5891
SacI G_AGCT'C
Cuts at: 2905 2905
Size: 5891
SalI G'TCGA_C
Cuts at: 2928 2928
Size: 5891
SapI GCTCTTCn'nnn_
Cuts at: 3914 4124 3914
Size: 210 5681
SbfI CC_TGCA'GG
Cuts at: 2938 2938
Size: 5891
ScaI AGT'ACT
Cuts at: 5408 5408
Size: 5891
SfcI C'TryA_G
Cuts at: 33 623 1280 1594 2211 2294 2667 2934 3619
Size: 590 657 314 617 83 373 267 685
Cuts at: 3619 4612 4803 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 657 617 590 373 314
267 191 83
SfoI GGC'GCC
Cuts at: 3323 3571 3323
Size: 248 5643
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5891
SmaI CCC'GGG
Cuts at: 2913 2913
Size: 5891
SmlI C'TyrA_G
Cuts at: 1651 2656 3046 3961 4453 4715 1651
Size: 1005 390 915 492 262 2827
Fragments arranged by size:
2827 1005 915 492 390 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5891
SphI G_CATG'C
Cuts at: 2472 2944 3444 2472
Size: 472 500 4919
Fragments arranged by size:
4919 500 472
SspI AAT'ATT
Cuts at: 1949 1949
Size: 5891
StyI C'CwwG_G
Cuts at: 107 1371 1435 1676 1883 3025 3379 107
Size: 1264 64 241 207 1142 354 2619
Fragments arranged by size:
2619 1264 1142 354 241 207 64
SwaI ATTT'AAAT
Cuts at: 1517 1517
Size: 5891
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2232 3474 3607 4066 4275 2232
Size: 1242 133 459 209 3848
Fragments arranged by size:
3848 1242 459 209 133
TatI w'GTAC_w
Cuts at: 415 5406 415
Size: 4991 900
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 481 513 996 1028 5408 5440 481
Size: 32 483 32 4380 32 932
Fragments arranged by size:
4380 932 483 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2145 3688 2145
Size: 1543 4348
XbaI T'CTAG_A
Cuts at: 2922 2922
Size: 5891
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 295 483 3386 295
Size: 188 2903 2800
Fragments arranged by size:
2903 2800 188
XmaI C'CCGG_G
Cuts at: 2911 2911
Size: 5891
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NsiI NspI PciI PfoI PmeI PpiI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI AscI AvrII
BbvCI BcgI BmgBI Bpu10I BsiWI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NotI NruI PacI PasI
PflMI PmlI PpuMI PshAI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI XhoI XmnI
ZraI