Restriction map for
pF1KE3703
(Circular) (Six-base) MAPSORT of: pF1KE3703.seq Check: 5354 from: 1 to: 8150
pF1KE3703 8150 bp
With 182 enzymes: SgfI *
May 29, 2012 11:57 ..
AatII G_ACGT'C
Cuts at: 3524 3524
Size: 8150
AccI GT'mk_AC
Cuts at: 5188 5188
Size: 8150
Acc65I G'GTAC_C
Cuts at: 5166 5166
Size: 8150
AclI AA'CG_TT
Cuts at: 7990 7990
Size: 8150
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1947 2293 3388 3427 5975 6407 7154 7422 1947
Size: 346 1095 39 2548 432 747 268 2675
Fragments arranged by size:
2675 2548 1095 747 432 346 268 39
AfeI AGC'GCT
Cuts at: 4365 4365
Size: 8150
AflIII A'CryG_T
Cuts at: 4646 4886 5502 6606 7583 4646
Size: 240 616 1104 977 5213
Fragments arranged by size:
5213 1104 977 616 240
AgeI A'CCGG_T
Cuts at: 6538 6538
Size: 8150
AleI CACnn'nnGTG
Cuts at: 473 3841 5005 473
Size: 3368 1164 3618
Fragments arranged by size:
3618 3368 1164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 977 3859 977
Size: 2882 5268
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4609 4641 4609
Size: 32 8118
AlwNI CAG_nnn'CTG
Cuts at: 3265 3301 3509 4137 5455 7022 3265
Size: 36 208 628 1318 1567 4393
Fragments arranged by size:
4393 1567 1318 628 208 36
ApaI G_GGCC'C
Cuts at: 138 542 973 1418 2591 2729 2736 2773 3395
Size: 404 431 445 1173 138 7 37 622
Cuts at: 3395 4559 5016 138
Size: 1164 457 3272
Fragments arranged by size:
3272 1173 1164 622 457 445 431 404
138 37 7
ApaLI G'TGCA_C
Cuts at: 4336 4504 6920 4336
Size: 168 2416 5566
Fragments arranged by size:
5566 2416 168
ApoI r'AATT_y
Cuts at: 2158 5154 7503 7759 2158
Size: 2996 2349 256 2549
Fragments arranged by size:
2996 2549 2349 256
AseI AT'TA_AT
Cuts at: 20 4490 20
Size: 4470 3680
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8150
AvaI C'yCGr_G
Cuts at: 1580 2011 2071 2093 2735 2766 3320 3629 5170
Size: 431 60 22 642 31 554 309 1541
Cuts at: 5170 1580
Size: 4560
Fragments arranged by size:
4560 1541 642 554 431 309 60 31
22
BamHI G'GATC_C
Cuts at: 5175 5175
Size: 8150
BanI G'GyrC_C
Cuts at: 314 345 1282 1967 4084 4242 4919 5166 5580
Size: 31 937 685 2117 158 677 247 414
Cuts at: 5580 5828 5863 314
Size: 248 35 2601
Fragments arranged by size:
2601 2117 937 685 677 414 248 247
158 35 31
BanII G_rGCy'C
Cuts at: 138 196 542 973 1418 1455 1610 1981 2282
Size: 58 346 431 445 37 155 371 301
Cuts at: 2282 2591 2677 2729 2736 2773 3395 3760 3910
Size: 309 86 52 7 37 622 365 150
Cuts at: 3910 4559 5016 5080 5102 5164 6194 138
Size: 649 457 64 22 62 1030 2094
Fragments arranged by size:
2094 1030 649 622 457 445 431 371
365 346 309 301 155 150 86 64
62 58 52 37 37 22 7
BbeI G_GCGC'C
Cuts at: 349 1971 4923 5584 5832 349
Size: 1622 2952 661 248 2667
Fragments arranged by size:
2952 2667 1622 661 248
BbvCI CC'TCA_GC
Cuts at: 775 2652 775
Size: 1877 6273
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 4074 4108 4894 4928 90
Size: 34 3950 34 786 34 3312
Fragments arranged by size:
3950 3312 786 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6048 6809 8150 6048
Size: 761 1341 6048
Fragments arranged by size:
6048 1341 761
BclI T'GATC_A
Cuts at: 4703 5670 4703
Size: 967 7183
BfrBI ATG'CAT
Cuts at: 111 4435 111
Size: 4324 3826
BglI GCCn_nnn'nGGC
Cuts at: 785 1280 1573 3226 3755 4196 4940 5020 785
Size: 495 293 1653 529 441 744 80 3915
Fragments arranged by size:
3915 1653 744 529 495 441 293 80
BglII A'GATC_T
Cuts at: 1595 3890 5665 7702 1595
Size: 2295 1775 2037 2043
Fragments arranged by size:
2295 2043 2037 1775
BlpI GC'TnA_GC
Cuts at: 249 530 4109 4186 5262 249
Size: 281 3579 77 1076 3137
Fragments arranged by size:
3579 3137 1076 281 77
Bme1580I G_kGCm'C
Cuts at: 138 317 542 973 1418 1708 2591 2729 2736
Size: 179 225 431 445 290 883 138 7
Cuts at: 2736 2773 3395 4247 4340 4508 4559 4637 4917
Size: 37 622 852 93 168 51 78 280
Cuts at: 4917 5016 5046 5775 5868 6924 138
Size: 99 30 729 93 1056 1364
Fragments arranged by size:
1364 1056 883 852 729 622 445 431
290 280 225 179 168 138 99 93
93 78 51 37 30 7
BmgBI CAC'GTC
Cuts at: 5138 5138
Size: 8150
BmrI ACTGGGnnnn_n'
Cuts at: 712 2905 3582 3876 4359 4518 5056 5362 5443
Size: 2193 677 294 483 159 538 306 81
Cuts at: 5443 5778 7922 712
Size: 335 2144 940
Fragments arranged by size:
2193 2144 940 677 538 483 335 306
294 159 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6517 6517
Size: 8150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 113 210 257 323 646 803 1034 1197 1408
Size: 97 47 66 323 157 231 163 211
Cuts at: 1408 1904 2152 2551 2582 3230 3688 4975 113
Size: 496 248 399 31 648 458 1287 3288
Fragments arranged by size:
3288 1287 648 496 458 399 323 248
231 211 163 157 97 66 47 31
Bpu10I CC'TnA_GC
Cuts at: 172 775 2652 4982 5096 172
Size: 603 1877 2330 114 3226
Fragments arranged by size:
3226 2330 1877 603 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 5326 6205 6697 6995 673
Size: 4653 879 492 298 1828
Fragments arranged by size:
4653 1828 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1330 1398 1998 2857 3439 4348 4469 7854 1330
Size: 68 600 859 582 909 121 3385 1626
Fragments arranged by size:
3385 1626 909 859 600 582 121 68
BsaAI yAC'GTr
Cuts at: 4194 4647 4889 5503 4194
Size: 453 242 614 6841
Fragments arranged by size:
6841 614 453 242
BsaBI GATnn'nnATC
Cuts at: 6 5664 7701 6
Size: 5658 2037 455
Fragments arranged by size:
5658 2037 455
BsaHI Gr'CG_yC
Cuts at: 346 1968 3521 4920 4994 5581 5829 6531 8027
Size: 1622 1553 1399 74 587 248 702 1496
Cuts at: 8027 346
Size: 469
Fragments arranged by size:
1622 1553 1496 1399 702 587 469 248
74
BsaWI w'CCGG_w
Cuts at: 5339 5860 6538 6812 6959 7370 5339
Size: 521 678 274 147 411 6119
Fragments arranged by size:
6119 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 619 649 1637 1667 2125 2155 2319 2349 2555
Size: 30 988 30 458 30 164 30 206
Cuts at: 2555 2585 4273 4303 4466 4496 619
Size: 30 1688 30 163 30 4273
Fragments arranged by size:
4273 1688 988 458 206 164 163 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 206 493 781 2161 2413 2992 3190 3631 3994
Size: 287 288 1380 252 579 198 441 363
Cuts at: 3994 4015 4024 4987 5052 206
Size: 21 9 963 65 3304
Fragments arranged by size:
3304 1380 963 579 441 363 288 287
252 198 65 21 9
BseYI C'CCAG_C
Cuts at: 181 319 422 534 566 1044 1795 1861 1866
Size: 138 103 112 32 478 751 66 5
Cuts at: 1866 2057 2491 2901 3150 3278 3655 4124 5127
Size: 191 434 410 249 128 377 469 1003
Cuts at: 5127 5575 6333 6910 7406 181
Size: 448 758 577 496 925
Fragments arranged by size:
1003 925 758 751 577 496 478 469
448 434 410 377 249 191 138 128
112 103 66 32 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 418 745 3251 3865 4246 4483 4534 128
Size: 290 327 2506 614 381 237 51 3744
Fragments arranged by size:
3744 2506 614 381 327 290 237 51
BsiEI CG_ry'CG
Cuts at: 104 5084 5692 5738 6946 104
Size: 4980 608 46 1208 1308
Fragments arranged by size:
4980 1308 1208 608 46
BsiHKAI G_wGCw'C
Cuts at: 196 470 659 1610 3910 4340 4346 4508 5164
Size: 274 189 951 2300 430 6 162 656
Cuts at: 5164 5942 6132 6924 196
Size: 778 190 792 1422
Fragments arranged by size:
2300 1422 951 792 778 656 430 274
190 189 162 6
BsmI GAATG_Cn'
Cuts at: 1352 3546 1352
Size: 2194 5956
Bsp1286I G_dGCh'C
Cuts at: 138 196 317 470 542 659 973 1418 1455
Size: 58 121 153 72 117 314 445 37
Cuts at: 1455 1610 1708 1981 2282 2591 2677 2729 2736
Size: 155 98 273 301 309 86 52 7
Cuts at: 2736 2773 3395 3760 3910 4247 4340 4346 4508
Size: 37 622 365 150 337 93 6 162
Cuts at: 4508 4559 4637 4917 5016 5046 5080 5102 5164
Size: 51 78 280 99 30 34 22 62
Cuts at: 5164 5775 5868 5942 6132 6194 6924 138
Size: 611 93 74 190 62 730 1364
Fragments arranged by size:
1364 730 622 611 445 365 337 314
309 301 280 273 190 162 155 153
150 121 117 99 98 93 93 86
78 74 72 62 62 58 52 51
37 37 34 30 22 7 6
BspEI T'CCGG_A
Cuts at: 7370 7370
Size: 8150
BspHI T'CATG_A
Cuts at: 2192 7326 2192
Size: 5134 3016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3173 5200 5414 5716 6097 3173
Size: 2027 214 302 381 5226
Fragments arranged by size:
5226 2027 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2877 3474 6442 6496 7980 8145 50
Size: 2827 597 2968 54 1484 165 55
Fragments arranged by size:
2968 2827 1484 597 165 55 54
BsrDI GCAATG_nn'
Cuts at: 403 3926 4538 6062 403
Size: 3523 612 1524 2491
Fragments arranged by size:
3523 2491 1524 612
BsrFI r'CCGG_y
Cuts at: 1079 4522 5106 6148 6329 6538 1079
Size: 3443 584 1042 181 209 2691
Fragments arranged by size:
3443 2691 1042 584 209 181
BsrGI T'GTAC_A
Cuts at: 3712 4232 3712
Size: 520 7630
BssHII G'CGCG_C
Cuts at: 3528 5023 3528
Size: 1495 6655
BssSI C'ACGA_G
Cuts at: 4339 4480 6421 6779 4339
Size: 141 1941 358 5710
Fragments arranged by size:
5710 1941 358 141
BstAPI GCAn_nnn'nTGC
Cuts at: 405 732 1023 3274 4137 4512 4829 5527 405
Size: 327 291 2251 863 375 317 698 3028
Fragments arranged by size:
3028 2251 863 698 375 327 317 291
BstBI TT'CG_AA
Cuts at: 6511 6511
Size: 8150
BstXI CCAn_nnnn'nTGG
Cuts at: 434 941 2130 3798 434
Size: 507 1189 1668 4786
Fragments arranged by size:
4786 1668 1189 507
BstYI r'GATC_y
Cuts at: 1 1595 2165 3179 3410 3870 3890 5175 5642
Size: 1594 570 1014 231 460 20 1285 467
Cuts at: 5642 5665 6000 6246 7258 7344 7356 7702 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1594 1285 1014 1012 570 467 460 449
346 335 246 231 86 23 20 12
Bsu36I CC'TnA_GG
Cuts at: 759 1953 2718 759
Size: 1194 765 6191
Fragments arranged by size:
6191 1194 765
BtgI C'CryG_G
Cuts at: 366 1137 1401 2381 3824 4172 366
Size: 771 264 980 1443 348 4344
Fragments arranged by size:
4344 1443 980 771 348 264
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1645 4847 6100 6281 6444 1645
Size: 3202 1253 181 163 3351
Fragments arranged by size:
3351 3202 1253 181 163
BtsI GCAGTG_nn'
Cuts at: 125 841 3005 4734 4899 5393 125
Size: 716 2164 1729 165 494 2882
Fragments arranged by size:
2882 2164 1729 716 494 165
DraI TTT'AAA
Cuts at: 5149 5149
Size: 8150
DraIII CAC_nnn'GTG
Cuts at: 3346 3385 3825 4386 4512 3346
Size: 39 440 561 126 6984
Fragments arranged by size:
6984 561 440 126 39
DrdI GACnn_nn'nnGTC
Cuts at: 5856 6714 5856
Size: 858 7292
EaeI y'GGCC_r
Cuts at: 549 1074 1404 1829 2183 2384 2498 4411 5081
Size: 525 330 425 354 201 114 1913 670
Cuts at: 5081 5735 5909 6300 6327 7672 549
Size: 654 174 391 27 1345 1027
Fragments arranged by size:
1913 1345 1027 670 654 525 425 391
354 330 201 174 114 27
EagI C'GGCC_G
Cuts at: 5081 5735 5081
Size: 654 7496
EarI CTCTTCn'nnn_
Cuts at: 1598 1615 2219 2390 2750 2837 3464 3787 6173
Size: 17 604 171 360 87 627 323 2386
Cuts at: 6173 6383 1598
Size: 210 3365
Fragments arranged by size:
3365 2386 627 604 360 323 210 171
87 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6358 6668 6814 7960 6358
Size: 310 146 1146 6548
Fragments arranged by size:
6548 1146 310 146
EcoICRI GAG'CTC
Cuts at: 194 1608 3908 5162 194
Size: 1414 2300 1254 3182
Fragments arranged by size:
3182 2300 1414 1254
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 113 210 257 323 646 803 1034 1197 1408
Size: 97 47 66 323 157 231 163 211
Cuts at: 1408 1904 1947 2152 2293 2551 2582 3230 3388
Size: 496 43 205 141 258 31 648 158
Cuts at: 3388 3427 3688 4975 5975 6407 7154 7422 113
Size: 39 261 1287 1000 432 747 268 841
Fragments arranged by size:
1287 1000 841 747 648 496 432 323
268 261 258 231 211 205 163 158
157 141 97 66 47 43 39 31
EcoNI CCTnn'n_nnAGG
Cuts at: 2093 2093
Size: 8150
EcoO109I rG'GnC_Cy
Cuts at: 134 168 185 538 539 612 688 754 771
Size: 34 17 353 1 73 76 66 17
Cuts at: 771 969 1111 1414 1415 1627 1678 1884 2038
Size: 198 142 303 1 212 51 206 154
Cuts at: 2038 2098 2588 2726 2770 3123 3218 3391 3392
Size: 60 490 138 44 353 95 173 1
Cuts at: 3392 3747 4555 4556 5012 5124 5289 134
Size: 355 808 1 456 112 165 2995
Fragments arranged by size:
2995 808 490 456 355 353 353 303
212 206 198 173 165 154 142 138
112 95 76 73 66 60 51 44
34 17 17 1 1 1 1
EcoRI G'AATT_C
Cuts at: 2158 5154 2158
Size: 2996 5154
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2298 3465 6169 2298
Size: 1167 2704 4279
Fragments arranged by size:
4279 2704 1167
FspI TGC'GCA
Cuts at: 3484 5931 3484
Size: 2447 5703
HaeII r_GCGC'y
Cuts at: 349 506 1005 1364 1971 2669 4367 4923 5584
Size: 157 499 359 607 698 1698 556 661
Cuts at: 5584 5832 6854 7833 349
Size: 248 1022 979 666
Fragments arranged by size:
1698 1022 979 698 666 661 607 556
499 359 248 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 596 628 3171 3203 3673 3705 3867 3899 3988
Size: 32 2543 32 470 32 162 32 89
Cuts at: 3988 4020 5992 6022 6024 6054 596
Size: 32 1972 30 2 30 2692
Fragments arranged by size:
2692 2543 1972 470 162 89 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 4296 5189 4296
Size: 893 7257
HindIII A'AGCT_T
Cuts at: 522 1959 522
Size: 1437 6713
KasI G'GCGC_C
Cuts at: 345 1967 4919 5580 5828 345
Size: 1622 2952 661 248 2667
Fragments arranged by size:
2952 2667 1622 661 248
KpnI G_GTAC'C
Cuts at: 5170 5170
Size: 8150
MluI A'CGCG_T
Cuts at: 7583 7583
Size: 8150
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1414 6821 7005 1414
Size: 5407 184 2559
Fragments arranged by size:
5407 2559 184
MscI TGG'CCA
Cuts at: 1076 1831 2185 4413 5911 1076
Size: 755 354 2228 1498 3315
Fragments arranged by size:
3315 2228 1498 755 354
MslI CAynn'nnrTG
Cuts at: 392 473 959 2197 3736 3841 4240 5005 6139
Size: 81 486 1238 1539 105 399 765 1134
Cuts at: 6139 6266 392
Size: 127 2276
Fragments arranged by size:
2276 1539 1238 1134 765 486 399 127
105 81
MspA1I CmG'CkG
Cuts at: 1014 1459 2130 2271 2569 2656 2932 3011 4128
Size: 445 671 141 298 87 276 79 1117
Cuts at: 4128 4230 4581 5034 5261 5575 5935 6948 7193
Size: 102 351 453 227 314 360 1013 245
Cuts at: 7193 1014
Size: 1971
Fragments arranged by size:
1971 1117 1013 671 453 445 360 351
314 298 276 245 227 141 102 87
79
NaeI GCC'GGC
Cuts at: 5108 6331 5108
Size: 1223 6927
NarI GG'CG_CC
Cuts at: 346 1968 4920 5581 5829 346
Size: 1622 2952 661 248 2667
Fragments arranged by size:
2952 2667 1622 661 248
NcoI C'CATG_G
Cuts at: 366 1137 1401 366
Size: 771 264 7115
Fragments arranged by size:
7115 771 264
NgoMIV G'CCGG_C
Cuts at: 5106 6329 5106
Size: 1223 6927
NruI TCG'CGA
Cuts at: 1664 5142 1664
Size: 3478 4672
NsiI A_TGCA'T
Cuts at: 113 4437 113
Size: 4324 3826
NspI r_CATG'y
Cuts at: 3366 4185 5203 5703 6610 3366
Size: 819 1018 500 907 4906
Fragments arranged by size:
4906 1018 907 819 500
PasI CC'CwG_GG
Cuts at: 832 1988 2670 2729 3334 832
Size: 1156 682 59 605 5648
Fragments arranged by size:
5648 1156 682 605 59
PciI A'CATG_T
Cuts at: 6606 6606
Size: 8150
PflMI CCAn_nnn'nTGG
Cuts at: 1083 4526 1083
Size: 3443 4707
PfoI T'CCnGG_A
Cuts at: 410 3962 4418 410
Size: 3552 456 4142
Fragments arranged by size:
4142 3552 456
PmeI GTTT'AAAC
Cuts at: 5149 5149
Size: 8150
PmlI CAC'GTG
Cuts at: 4194 4647 4194
Size: 453 7697
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3897 3929 7289 7321 3897
Size: 32 3360 32 4726
Fragments arranged by size:
4726 3360 32 32
PpuMI rG'GwC_Cy
Cuts at: 612 688 1627 1884 3123 612
Size: 76 939 257 1239 5639
Fragments arranged by size:
5639 1239 939 257 76
PspOMI G'GGCC_C
Cuts at: 134 538 969 1414 2587 2725 2732 2769 3391
Size: 404 431 445 1173 138 7 37 622
Cuts at: 3391 4555 5012 134
Size: 1164 457 3272
Fragments arranged by size:
3272 1173 1164 622 457 445 431 404
138 37 7
PstI C_TGCA'G
Cuts at: 560 744 2297 3170 3707 3977 4133 4724 4940
Size: 184 1553 873 537 270 156 591 216
Cuts at: 4940 5061 5197 5882 560
Size: 121 136 685 2828
Fragments arranged by size:
2828 1553 873 685 591 537 270 216
184 156 136 121
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8150
PvuII CAG'CTG
Cuts at: 2569 3011 4128 4230 4581 5575 5935 2569
Size: 442 1117 102 351 994 360 4784
Fragments arranged by size:
4784 1117 994 442 360 351 102
RsrII CG'GwC_CG
Cuts at: 6345 6345
Size: 8150
SacI G_AGCT'C
Cuts at: 196 1610 3910 5164 196
Size: 1414 2300 1254 3182
Fragments arranged by size:
3182 2300 1414 1254
SalI G'TCGA_C
Cuts at: 5187 5187
Size: 8150
SanDI GG'GwC_CC
Cuts at: 1627 1627
Size: 8150
SapI GCTCTTCn'nnn_
Cuts at: 1598 1615 2750 3464 6173 6383 1598
Size: 17 1135 714 2709 210 3365
Fragments arranged by size:
3365 2709 1135 714 210 17
SbfI CC_TGCA'GG
Cuts at: 4940 5197 4940
Size: 257 7893
ScaI AGT'ACT
Cuts at: 2545 7667 2545
Size: 5122 3028
SexAI A'CCwGG_T
Cuts at: 4892 4892
Size: 8150
SfcI C'TryA_G
Cuts at: 33 556 740 2293 3166 3703 3973 4129 4720
Size: 523 184 1553 873 537 270 156 591
Cuts at: 4720 4936 5057 5193 5878 6871 7062 33
Size: 216 121 136 685 993 191 1121
Fragments arranged by size:
1553 1121 993 873 685 591 537 523
270 216 191 184 156 136 121
SfiI GGCCn_nnn'nGGCC
Cuts at: 3226 3226
Size: 8150
SfoI GGC'GCC
Cuts at: 347 1969 4921 5582 5830 347
Size: 1622 2952 661 248 2667
Fragments arranged by size:
2952 2667 1622 661 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8150
SmaI CCC'GGG
Cuts at: 2073 2737 2768 3631 5172 2073
Size: 664 31 863 1541 5051
Fragments arranged by size:
5051 1541 863 664 31
SmlI C'TyrA_G
Cuts at: 652 5305 6220 6712 6974 652
Size: 4653 915 492 262 1828
Fragments arranged by size:
4653 1828 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8150
SphI G_CATG'C
Cuts at: 5203 5703 5203
Size: 500 7650
SspI AAT'ATT
Cuts at: 4443 4443
Size: 8150
StuI AGG'CCT
Cuts at: 3952 4934 4987 3952
Size: 982 53 7115
Fragments arranged by size:
7115 982 53
StyI C'CwwG_G
Cuts at: 162 366 608 1137 1401 2516 2972 3113 3119
Size: 204 242 529 264 1115 456 141 6
Cuts at: 3119 3224 3398 3545 5284 5638 162
Size: 105 174 147 1739 354 2674
Fragments arranged by size:
2674 1739 1115 529 456 354 264 242
204 174 147 141 105 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1046 2404 2800 3252 3669 3853 4500 5733 5866
Size: 1358 396 452 417 184 647 1233 133
Cuts at: 5866 6325 6534 1046
Size: 459 209 2662
Fragments arranged by size:
2662 1358 1233 647 459 452 417 396
209 184 133
TatI w'GTAC_w
Cuts at: 2543 3712 4232 7665 2543
Size: 1169 520 3433 3028
Fragments arranged by size:
3433 3028 1169 520
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 619 651 1637 1669 2319 2351 4466 4498 7667
Size: 32 986 32 650 32 2115 32 3169
Cuts at: 7667 7699 619
Size: 32 1070
Fragments arranged by size:
3169 2115 1070 986 650 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 5947 5947
Size: 8150
XbaI T'CTAG_A
Cuts at: 5181 5181
Size: 8150
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1547 3663 3795 3798 5645 1547
Size: 2116 132 3 1847 4052
Fragments arranged by size:
4052 2116 1847 132 3
XmaI C'CCGG_G
Cuts at: 2071 2735 2766 3629 5170 2071
Size: 664 31 863 1541 5051
Fragments arranged by size:
5051 1541 863 664 31
XmnI GAAnn'nnTTC
Cuts at: 1441 2213 1441
Size: 772 7378
ZraI GAC'GTC
Cuts at: 3522 3522
Size: 8150
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AscI AvrII BaeI BbsI BmtI
BsiWI BsmBI BstEII BstZ17I ClaI CspCI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI PacI PshAI
PsiI PspXI PsrI SacII SgrAI SnaBI SrfI SwaI
XhoI