Restriction map for
pF1KE3717
(Circular) (Six-base) MAPSORT of: pF1KE3717.seq Check: 8233 from: 1 to: 10259
pF1KE3717 10259 bp
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AatII G_ACGT'C
Cuts at: 1024 6826 1024
Size: 5802 4457
AccI GT'mk_AC
Cuts at: 812 2662 3311 3732 4014 6412 6869 7297 812
Size: 1850 649 421 282 2398 457 428 3774
Fragments arranged by size:
3774 2398 1850 649 457 428 421 282
Acc65I G'GTAC_C
Cuts at: 1261 7275 1261
Size: 6014 4245
AclI AA'CG_TT
Cuts at: 10099 10099
Size: 10259
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 1096 1249 1478 1949 1955 2015 3320 4265
Size: 180 153 229 471 6 60 1305 945
Cuts at: 4265 4274 4439 4460 5036 5846 6566 8084 8516
Size: 9 165 21 576 810 720 1518 432
Cuts at: 8516 9263 9531 916
Size: 747 268 1644
Fragments arranged by size:
1644 1518 1305 945 810 747 720 576
471 432 268 229 180 165 153 60
21 9 6
AflII C'TTAA_G
Cuts at: 4125 6661 4125
Size: 2536 7723
AflIII A'CryG_T
Cuts at: 7611 8715 9692 7611
Size: 1104 977 8178
Fragments arranged by size:
8178 1104 977
AgeI A'CCGG_T
Cuts at: 8647 8647
Size: 10259
AhdI GACnn_n'nnGTC
Cuts at: 6297 6297
Size: 10259
AleI CACnn'nnGTG
Cuts at: 2567 3088 4279 4558 2567
Size: 521 1191 279 8268
Fragments arranged by size:
8268 1191 521 279
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2510 2542 3131 3163 3769 3801 5505 5537 2510
Size: 32 589 32 606 32 1704 32 7232
Fragments arranged by size:
7232 1704 606 589 32 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 526 2724 3955 4177 4483 5662 5917 6375 7564
Size: 2198 1231 222 306 1179 255 458 1189
Cuts at: 7564 9131 526
Size: 1567 1654
Fragments arranged by size:
2198 1654 1567 1231 1189 1179 458 306
255 222
ApaI G_GGCC'C
Cuts at: 559 6882 6943 559
Size: 6323 61 3875
Fragments arranged by size:
6323 3875 61
ApaLI G'TGCA_C
Cuts at: 3317 9029 3317
Size: 5712 4547
ApoI r'AATT_y
Cuts at: 3470 3550 7188 7263 9612 9868 3470
Size: 80 3638 75 2349 256 3861
Fragments arranged by size:
3861 3638 2349 256 80 75
AseI AT'TA_AT
Cuts at: 20 1798 20
Size: 1778 8481
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10259
AvaI C'yCGr_G
Cuts at: 2108 2763 3574 6881 7279 2108
Size: 655 811 3307 398 5088
Fragments arranged by size:
5088 3307 811 655 398
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2014 2047 2950 2983 2014
Size: 33 903 33 9290
Fragments arranged by size:
9290 903 33 33
BamHI G'GATC_C
Cuts at: 2086 7284 2086
Size: 5198 5061
BanI G'GyrC_C
Cuts at: 118 272 1088 1261 5710 6429 6712 7275 7689
Size: 154 816 173 4449 719 283 563 414
Cuts at: 7689 7937 7972 118
Size: 248 35 2405
Fragments arranged by size:
4449 2405 816 719 563 414 283 248
173 154 35
BanII G_rGCy'C
Cuts at: 464 559 3976 5121 6220 6882 6943 7273 8303
Size: 95 3417 1145 1099 662 61 330 1030
Cuts at: 8303 464
Size: 2420
Fragments arranged by size:
3417 2420 1145 1099 1030 662 330 95
61
BbeI G_GCGC'C
Cuts at: 122 276 7693 7941 122
Size: 154 7417 248 2440
Fragments arranged by size:
7417 2440 248 154
BbsI GAAGACnn'nnnn_
Cuts at: 922 1102 2088 3800 6131 6323 6483 922
Size: 180 986 1712 2331 192 160 4698
Fragments arranged by size:
4698 2331 1712 986 192 180 160
BbvCI CC'TCA_GC
Cuts at: 2324 2903 2324
Size: 579 9680
BciVI GTATCCnnnnn_n'
Cuts at: 4460 4915 8157 8918 10259 4460
Size: 455 3242 761 1341 4460
Fragments arranged by size:
4460 3242 1341 761 455
BclI T'GATC_A
Cuts at: 1755 3714 5531 7779 1755
Size: 1959 1817 2248 4235
Fragments arranged by size:
4235 2248 1959 1817
BfrBI ATG'CAT
Cuts at: 1484 1484
Size: 10259
BglI GCCn_nnn'nGGC
Cuts at: 1190 4803 5902 1190
Size: 3613 1099 5547
Fragments arranged by size:
5547 3613 1099
BglII A'GATC_T
Cuts at: 1397 7774 9811 1397
Size: 6377 2037 1845
Fragments arranged by size:
6377 2037 1845
BlpI GC'TnA_GC
Cuts at: 4633 5120 6214 6806 7371 4633
Size: 487 1094 592 565 7521
Fragments arranged by size:
7521 1094 592 565 487
Bme1580I G_kGCm'C
Cuts at: 559 3321 4047 4241 5715 5883 6434 6841 6882
Size: 2762 726 194 1474 168 551 407 41
Cuts at: 6882 6926 6943 7072 7884 7977 9033 559
Size: 44 17 129 812 93 1056 1785
Fragments arranged by size:
2762 1785 1474 1056 812 726 551 407
194 168 129 93 44 41 17
BmgBI CAC'GTC
Cuts at: 4042 4042
Size: 10259
BmrI ACTGGGnnnn_n'
Cuts at: 232 595 2036 2524 3926 5311 5463 5927 6430
Size: 363 1441 488 1402 1385 152 464 503
Cuts at: 6430 7471 7552 7887 10031 232
Size: 1041 81 335 2144 460
Fragments arranged by size:
2144 1441 1402 1385 1041 503 488 464
460 363 335 152 81
BmtI G_CTAG'C
Cuts at: 1937 1937
Size: 10259
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 158 2453 3452 8626 158
Size: 2295 999 5174 1791
Fragments arranged by size:
5174 2295 1791 999
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 177 406 503 748 2385 2942 4160 4280 4505
Size: 229 97 245 1637 557 1218 120 225
Cuts at: 4505 4787 5135 5161 6660 6725 177
Size: 282 348 26 1499 65 3711
Fragments arranged by size:
3711 1637 1499 1218 557 348 282 245
229 225 120 97 65 26
Bpu10I CC'TnA_GC
Cuts at: 2324 2903 4514 2324
Size: 579 1611 8069
Fragments arranged by size:
8069 1611 579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1366 2223 2262 2423 2444 3836 5606 7435
Size: 612 857 39 161 21 1392 1770 1829
Cuts at: 7435 8314 8806 9104 754
Size: 879 492 298 1909
Fragments arranged by size:
1909 1829 1770 1392 879 857 612 492
298 161 39 21
BsaI GGTCTCn'nnnn_
Cuts at: 469 724 1202 3488 4303 5093 5137 9963 469
Size: 255 478 2286 815 790 44 4826 765
Fragments arranged by size:
4826 2286 815 790 765 478 255 44
BsaAI yAC'GTr
Cuts at: 1621 7612 1621
Size: 5991 4268
BsaBI GATnn'nnATC
Cuts at: 6 1066 2015 6353 7773 9810 6
Size: 1060 949 4338 1420 2037 455
Fragments arranged by size:
4338 2037 1420 1060 949 455
BsaHI Gr'CG_yC
Cuts at: 119 273 1021 6823 7690 7938 8640 10136 119
Size: 154 748 5802 867 248 702 1496 242
Fragments arranged by size:
5802 1496 867 748 702 248 242 154
BsaWI w'CCGG_w
Cuts at: 456 5385 6187 7448 7969 8647 8921 9068 9479
Size: 4929 802 1261 521 678 274 147 411
Cuts at: 9479 456
Size: 1236
Fragments arranged by size:
4929 1261 1236 802 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1116 1146 1550 1580 2340 2370 2582 2612 3131
Size: 30 404 30 760 30 212 30 519
Cuts at: 3131 3161 3485 3515 4059 4089 4635 4665 4760
Size: 30 324 30 544 30 546 30 95
Cuts at: 4760 4790 5367 5397 5651 5681 1116
Size: 30 577 30 254 30 5694
Fragments arranged by size:
5694 760 577 546 544 519 404 324
254 212 95 30 30 30 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 277 649 1309 1852 2348 2642 3592 6032 6264
Size: 372 660 543 496 294 950 2440 232
Cuts at: 6264 277
Size: 4272
Fragments arranged by size:
4272 2440 950 660 543 496 372 294
232
BseYI C'CCAG_C
Cuts at: 416 421 509 577 608 2492 3158 3200 3284
Size: 5 88 68 31 1884 666 42 84
Cuts at: 3284 5428 5474 6453 6545 6596 7134 7684 8442
Size: 2144 46 979 92 51 538 550 758
Cuts at: 8442 9019 9515 416
Size: 577 496 1160
Fragments arranged by size:
2144 1884 1160 979 758 666 577 550
538 496 92 88 84 68 51 46
42 31 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 1376 2392 2402 3112 4727 6596 6950 365
Size: 1011 1016 10 710 1615 1869 354 3674
Fragments arranged by size:
3674 1869 1615 1016 1011 710 354 10
BsiEI CG_ry'CG
Cuts at: 104 236 314 7801 7847 9055 104
Size: 132 78 7487 46 1208 1308
Fragments arranged by size:
7487 1308 1208 132 78 46
BsiHKAI G_wGCw'C
Cuts at: 338 453 2574 3321 5121 5167 6220 7273 8051
Size: 115 2121 747 1800 46 1053 1053 778
Cuts at: 8051 8241 9033 338
Size: 190 792 1564
Fragments arranged by size:
2121 1800 1564 1053 1053 792 778 747
190 115 46
BsmI GAATG_Cn'
Cuts at: 3830 3830
Size: 10259
Bsp1286I G_dGCh'C
Cuts at: 338 453 464 559 2574 3321 3976 4047 4241
Size: 115 11 95 2015 747 655 71 194
Cuts at: 4241 5121 5167 5715 5883 6220 6434 6841 6882
Size: 880 46 548 168 337 214 407 41
Cuts at: 6882 6926 6943 7072 7273 7884 7977 8051 8241
Size: 44 17 129 201 611 93 74 190
Cuts at: 8241 8303 9033 338
Size: 62 730 1564
Fragments arranged by size:
2015 1564 880 747 730 655 611 548
407 337 214 201 194 190 168 129
115 95 93 74 71 62 46 44
41 17 11
BspEI T'CCGG_A
Cuts at: 9479 9479
Size: 10259
BspHI T'CATG_A
Cuts at: 9435 9435
Size: 10259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1872 4371 5407 6383 7250 7309 7523 7825 8206
Size: 2499 1036 976 867 59 214 302 381
Cuts at: 8206 1872
Size: 3925
Fragments arranged by size:
3925 2499 1036 976 867 381 302 214
59
BsrBI CCG'CTC
Cuts at: 50 2598 8551 8605 10089 10254 50
Size: 2548 5953 54 1484 165 55
Fragments arranged by size:
5953 2548 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3360 4133 8171 3360
Size: 773 4038 5448
Fragments arranged by size:
5448 4038 773
BsrFI r'CCGG_y
Cuts at: 168 250 645 1180 1939 8257 8438 8647 168
Size: 82 395 535 759 6318 181 209 1780
Fragments arranged by size:
6318 1780 759 535 395 209 181 82
BsrGI T'GTAC_A
Cuts at: 4944 4944
Size: 10259
BssSI C'ACGA_G
Cuts at: 8530 8888 8530
Size: 358 9901
BstAPI GCAn_nnn'nTGC
Cuts at: 5710 7636 5710
Size: 1926 8333
BstBI TT'CG_AA
Cuts at: 8620 8620
Size: 10259
BstEII G'GTnAC_C
Cuts at: 6556 6556
Size: 10259
BstXI CCAn_nnnn'nTGG
Cuts at: 384 741 2700 6616 384
Size: 357 1959 3916 4027
Fragments arranged by size:
4027 3916 1959 357
BstYI r'GATC_y
Cuts at: 1 1097 1397 2086 2525 5490 7284 7751 7774
Size: 1096 300 689 439 2965 1794 467 23
Cuts at: 7774 8109 8355 9367 9453 9465 9811 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
2965 1794 1096 1012 689 467 449 439
346 335 300 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 6413 6413
Size: 10259
Bsu36I CC'TnA_GG
Cuts at: 2003 2003
Size: 10259
BtgI C'CryG_G
Cuts at: 209 230 303 431 740 839 1766 2220 2870
Size: 21 73 128 309 99 927 454 650
Cuts at: 2870 4670 5564 209
Size: 1800 894 4904
Fragments arranged by size:
4904 1800 927 894 650 454 309 128
99 73 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 8209 8390 8553 8209
Size: 181 163 9915
Fragments arranged by size:
9915 181 163
BtsI GCAGTG_nn'
Cuts at: 377 7502 377
Size: 7125 3134
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2435 2470 5951 5986 2435
Size: 35 3481 35 6708
Fragments arranged by size:
6708 3481 35 35
DraI TTT'AAA
Cuts at: 903 1056 7258 903
Size: 153 6202 3904
Fragments arranged by size:
6202 3904 153
DrdI GACnn_nn'nnGTC
Cuts at: 7965 8823 7965
Size: 858 9401
EaeI y'GGCC_r
Cuts at: 233 311 759 7152 7844 8018 8409 8436 9781
Size: 78 448 6393 692 174 391 27 1345
Cuts at: 9781 233
Size: 711
Fragments arranged by size:
6393 1345 711 692 448 391 174 78
27
EagI C'GGCC_G
Cuts at: 233 311 7844 233
Size: 78 7533 2648
Fragments arranged by size:
7533 2648 78
EarI CTCTTCn'nnn_
Cuts at: 467 716 1856 3000 4241 4761 5865 8282 8492
Size: 249 1140 1144 1241 520 1104 2417 210
Cuts at: 8492 467
Size: 2234
Fragments arranged by size:
2417 2234 1241 1144 1140 1104 520 249
210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 337 8467 8777 8923 10069 337
Size: 8130 310 146 1146 527
Fragments arranged by size:
8130 1146 527 310 146
EcoICRI GAG'CTC
Cuts at: 5119 6218 7271 5119
Size: 1099 1053 8107
Fragments arranged by size:
8107 1099 1053
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 177 406 503 748 916 1096 1249 1478 1949
Size: 229 97 245 168 180 153 229 471
Cuts at: 1949 1955 2015 2385 2942 3320 4160 4265 4274
Size: 6 60 370 557 378 840 105 9
Cuts at: 4274 4280 4439 4460 4505 4787 5036 5135 5161
Size: 6 159 21 45 282 249 99 26
Cuts at: 5161 5846 6566 6660 6725 8084 8516 9263 9531
Size: 685 720 94 65 1359 432 747 268
Cuts at: 9531 177
Size: 905
Fragments arranged by size:
1359 905 840 747 720 685 557 471
432 378 370 282 268 249 245 229
229 180 168 159 153 105 99 97
94 65 60 45 26 21 9 6
6
EcoNI CCTnn'n_nnAGG
Cuts at: 4769 5450 4769
Size: 681 9578
EcoO109I rG'GnC_Cy
Cuts at: 556 4060 4510 4867 5455 5635 6372 6939 6940
Size: 3504 450 357 588 180 737 567 1
Cuts at: 6940 7398 556
Size: 458 3417
Fragments arranged by size:
3504 3417 737 588 567 458 450 357
180 1
EcoRI G'AATT_C
Cuts at: 7263 7263
Size: 10259
EcoRV GAT'ATC
Cuts at: 717 6306 717
Size: 5589 4670
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4628 6088 8278 4628
Size: 1460 2190 6609
Fragments arranged by size:
6609 2190 1460
FspI TGC'GCA
Cuts at: 8040 8040
Size: 10259
HaeII r_GCGC'y
Cuts at: 122 276 677 7112 7693 7941 8963 9942 122
Size: 154 401 6435 581 248 1022 979 439
Fragments arranged by size:
6435 1022 979 581 439 401 248 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1389 1421 1499 1531 1777 1809 2078 2110 2250
Size: 32 78 32 246 32 269 32 140
Cuts at: 2250 2282 2340 2372 2582 2614 3616 3648 4687
Size: 32 58 32 210 32 1002 32 1039
Cuts at: 4687 4719 5734 5766 5893 5925 6283 6315 8101
Size: 32 1015 32 127 32 358 32 1786
Cuts at: 8101 8131 8133 8163 1389
Size: 30 2 30 3485
Fragments arranged by size:
3485 1786 1039 1015 1002 358 269 246
210 140 127 78 58 32 32 32
32 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1317 2073 7298 1317
Size: 756 5225 4278
Fragments arranged by size:
5225 4278 756
KasI G'GCGC_C
Cuts at: 118 272 7689 7937 118
Size: 154 7417 248 2440
Fragments arranged by size:
7417 2440 248 154
KpnI G_GTAC'C
Cuts at: 1265 7279 1265
Size: 6014 4245
MfeI C'AATT_G
Cuts at: 5503 6873 5503
Size: 1370 8889
MluI A'CGCG_T
Cuts at: 9692 9692
Size: 10259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2821 3023 5599 5916 7185 7262 8930 9114 2821
Size: 202 2576 317 1269 77 1668 184 3966
Fragments arranged by size:
3966 2576 1668 1269 317 202 184 77
MscI TGG'CCA
Cuts at: 7154 8020 7154
Size: 866 9393
MslI CAynn'nnrTG
Cuts at: 2567 2698 3088 3326 4279 4558 6128 6988 8248
Size: 131 390 238 953 279 1570 860 1260
Cuts at: 8248 8375 2567
Size: 127 4451
Fragments arranged by size:
4451 1570 1260 953 860 390 279 238
131 127
MspA1I CmG'CkG
Cuts at: 232 433 526 662 1821 2470 2707 2755 2823
Size: 201 93 136 1159 649 237 48 68
Cuts at: 2823 4525 4620 5439 6213 6457 6648 7370 7684
Size: 1702 95 819 774 244 191 722 314
Cuts at: 7684 8044 9057 9302 232
Size: 360 1013 245 1189
Fragments arranged by size:
1702 1189 1159 1013 819 774 722 649
360 314 245 244 237 201 191 136
95 93 68 48
NaeI GCC'GGC
Cuts at: 170 647 8440 170
Size: 477 7793 1989
Fragments arranged by size:
7793 1989 477
NarI GG'CG_CC
Cuts at: 119 273 7690 7938 119
Size: 154 7417 248 2440
Fragments arranged by size:
7417 2440 248 154
NcoI C'CATG_G
Cuts at: 1766 2220 2870 5564 1766
Size: 454 650 2694 6461
Fragments arranged by size:
6461 2694 650 454
NdeI CA'TA_TG
Cuts at: 2136 2136
Size: 10259
NgoMIV G'CCGG_C
Cuts at: 168 645 8438 168
Size: 477 7793 1989
Fragments arranged by size:
7793 1989 477
NheI G'CTAG_C
Cuts at: 1933 1933
Size: 10259
NotI GC'GGCC_GC
Cuts at: 233 311 233
Size: 78 10181
NsiI A_TGCA'T
Cuts at: 1486 1486
Size: 10259
NspI r_CATG'y
Cuts at: 1344 5128 7312 7812 8719 1344
Size: 3784 2184 500 907 2884
Fragments arranged by size:
3784 2884 2184 907 500
PacI TTA_AT'TAA
Cuts at: 1802 1802
Size: 10259
PasI CC'CwG_GG
Cuts at: 535 2179 535
Size: 1644 8615
PciI A'CATG_T
Cuts at: 8715 8715
Size: 10259
PflMI CCAn_nnn'nTGG
Cuts at: 288 7167 288
Size: 6879 3380
PfoI T'CCnGG_A
Cuts at: 4764 5046 5451 4764
Size: 282 405 9572
Fragments arranged by size:
9572 405 282
PmeI GTTT'AAAC
Cuts at: 7258 7258
Size: 10259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3131 3163 5589 5621 9398 9430 3131
Size: 32 2426 32 3777 32 3960
Fragments arranged by size:
3960 3777 2426 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4060 4510 4867 5455 6372 4060
Size: 450 357 588 917 7947
Fragments arranged by size:
7947 917 588 450 357
PshAI GACnn'nnGTC
Cuts at: 1214 2078 1214
Size: 864 9395
PspOMI G'GGCC_C
Cuts at: 555 6878 6939 555
Size: 6323 61 3875
Fragments arranged by size:
6323 3875 61
PstI C_TGCA'G
Cuts at: 866 2810 3252 4368 4488 6380 7029 7306 7991
Size: 1944 442 1116 120 1892 649 277 685
Cuts at: 7991 866
Size: 3134
Fragments arranged by size:
3134 1944 1892 1116 685 649 442 277
120
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10259
PvuII CAG'CTG
Cuts at: 2470 2707 2755 2823 4525 4620 5439 6213 6457
Size: 237 48 68 1702 95 819 774 244
Cuts at: 6457 6648 7684 8044 2470
Size: 191 1036 360 4685
Fragments arranged by size:
4685 1702 1036 819 774 360 244 237
191 95 68 48
RsrII CG'GwC_CG
Cuts at: 8454 8454
Size: 10259
SacI G_AGCT'C
Cuts at: 5121 6220 7273 5121
Size: 1099 1053 8107
Fragments arranged by size:
8107 1099 1053
SacII CC_GC'GG
Cuts at: 233 434 233
Size: 201 10058
SalI G'TCGA_C
Cuts at: 7296 7296
Size: 10259
SapI GCTCTTCn'nnn_
Cuts at: 716 8282 8492 716
Size: 7566 210 2483
Fragments arranged by size:
7566 2483 210
SbfI CC_TGCA'GG
Cuts at: 7306 7306
Size: 10259
ScaI AGT'ACT
Cuts at: 3059 3222 4331 4497 4644 5232 9776 3059
Size: 163 1109 166 147 588 4544 3542
Fragments arranged by size:
4544 3542 1109 588 166 163 147
SfcI C'TryA_G
Cuts at: 33 862 2026 2165 2806 3026 3248 3308 3962
Size: 829 1164 139 641 220 222 60 654
Cuts at: 3962 4364 4484 4526 5957 6376 7025 7302 7987
Size: 402 120 42 1431 419 649 277 685
Cuts at: 7987 8980 9171 33
Size: 993 191 1121
Fragments arranged by size:
1431 1164 1121 993 829 685 654 649
641 419 402 277 222 220 191 139
120 60 42
SfoI GGC'GCC
Cuts at: 120 274 7691 7939 120
Size: 154 7417 248 2440
Fragments arranged by size:
7417 2440 248 154
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10259
SgrAI Cr'CCGG_yG
Cuts at: 645 645
Size: 10259
SmaI CCC'GGG
Cuts at: 2765 7281 2765
Size: 4516 5743
SmlI C'TyrA_G
Cuts at: 769 1345 2108 2238 2277 2438 2459 3851 4125
Size: 576 763 130 39 161 21 1392 274
Cuts at: 4125 5621 6661 7414 8329 8821 9083 769
Size: 1496 1040 753 915 492 262 1945
Fragments arranged by size:
1945 1496 1392 1040 915 763 753 576
492 274 262 161 130 39 21
SnaBI TAC'GTA
Cuts at: 1621 1621
Size: 10259
SpeI A'CTAG_T
Cuts at: 67 5151 5650 67
Size: 5084 499 4676
Fragments arranged by size:
5084 4676 499
SphI G_CATG'C
Cuts at: 1344 5128 7312 7812 1344
Size: 3784 2184 500 3791
Fragments arranged by size:
3791 3784 2184 500
SspI AAT'ATT
Cuts at: 945 2985 945
Size: 2040 8219
StuI AGG'CCT
Cuts at: 4024 4024
Size: 10259
StyI C'CwwG_G
Cuts at: 677 734 1766 2220 2511 2870 2889 4913 5564
Size: 57 1032 454 291 359 19 2024 651
Cuts at: 5564 5816 5930 6527 7393 7747 677
Size: 252 114 597 866 354 3189
Fragments arranged by size:
3189 2024 1032 866 651 597 454 359
354 291 252 114 57 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 274 1204 2855 3118 3512 6994 7842 7975 8434
Size: 930 1651 263 394 3482 848 133 459
Cuts at: 8434 8643 274
Size: 209 1890
Fragments arranged by size:
3482 1890 1651 930 848 459 394 263
209 133
TatI w'GTAC_w
Cuts at: 266 3057 3220 4329 4438 4495 4642 4714 4944
Size: 2791 163 1109 109 57 147 72 230
Cuts at: 4944 5230 9774 266
Size: 286 4544 751
Fragments arranged by size:
4544 2791 1109 751 286 230 163 147
109 72 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4059 4091 4388 4420 4760 4792 5165 5197 9776
Size: 32 297 32 340 32 373 32 4579
Cuts at: 9776 9808 4059
Size: 32 4510
Fragments arranged by size:
4579 4510 373 340 297 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 8056 8056
Size: 10259
XbaI T'CTAG_A
Cuts at: 5070 5484 7290 5070
Size: 414 1806 8039
Fragments arranged by size:
8039 1806 414
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4278 7754 4278
Size: 3476 6783
XhoI C'TCGA_G
Cuts at: 2108 2108
Size: 10259
XmaI C'CCGG_G
Cuts at: 2763 7279 2763
Size: 4516 5743
XmnI GAAnn'nnTTC
Cuts at: 6522 6522
Size: 10259
ZraI GAC'GTC
Cuts at: 1022 6824 1022
Size: 5802 4457
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AlfI AscI AvrII BcgI BsiWI BsmBI
BssHII ClaI DraIII FseI FspAI HindIII HpaI NruI
PmlI PsiI PspXI PsrI SanDI SexAI SfiI SrfI
SwaI