Restriction map for
pF1KE3768
(Circular) (Six-base) MAPSORT of: pF1KE3768.seq Check: 8875 from: 1 to: 5975
pF1KE3768 5975 bp
With 182 enzymes: SgfI *
April 23, 2019 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 519 702 1433 519
Size: 183 731 5061
Fragments arranged by size:
5061 731 183
AccI GT'mk_AC
Cuts at: 3013 3013
Size: 5975
Acc65I G'GTAC_C
Cuts at: 2991 2991
Size: 5975
AclI AA'CG_TT
Cuts at: 5815 5815
Size: 5975
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1855 3800 4232 4979 5247 1855
Size: 1945 432 747 268 2583
Fragments arranged by size:
2583 1945 747 432 268
AfeI AGC'GCT
Cuts at: 894 1345 894
Size: 451 5524
AflIII A'CryG_T
Cuts at: 1265 2380 3327 4431 5408 1265
Size: 1115 947 1104 977 1832
Fragments arranged by size:
1832 1115 1104 977 947
AgeI A'CCGG_T
Cuts at: 4363 4363
Size: 5975
AhdI GACnn_n'nnGTC
Cuts at: 777 777
Size: 5975
AleI CACnn'nnGTG
Cuts at: 2344 2344
Size: 5975
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1513 1996 1513
Size: 483 5492
AlwNI CAG_nnn'CTG
Cuts at: 1383 2697 3280 4847 1383
Size: 1314 583 1567 2511
Fragments arranged by size:
2511 1567 1314 583
ApaI G_GGCC'C
Cuts at: 164 453 1736 1790 2314 2879 2958 164
Size: 289 1283 54 524 565 79 3181
Fragments arranged by size:
3181 1283 565 524 289 79 54
ApaLI G'TGCA_C
Cuts at: 647 4745 647
Size: 4098 1877
ApoI r'AATT_y
Cuts at: 2979 5328 5584 2979
Size: 2349 256 3370
Fragments arranged by size:
3370 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5975
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5975
AvaI C'yCGr_G
Cuts at: 638 719 1093 1457 2043 2626 2760 2995 638
Size: 81 374 364 586 583 134 235 3618
Fragments arranged by size:
3618 586 583 374 364 235 134 81
BamHI G'GATC_C
Cuts at: 1597 3000 1597
Size: 1403 4572
BanI G'GyrC_C
Cuts at: 343 592 678 918 2055 2104 2170 2328 2579
Size: 249 86 240 1137 49 66 158 251
Cuts at: 2579 2991 3405 3653 3688 343
Size: 412 414 248 35 2630
Fragments arranged by size:
2630 1137 414 412 251 249 248 240
158 86 66 49 35
BanII G_rGCy'C
Cuts at: 164 453 561 1100 1736 1790 2039 2314 2633
Size: 289 108 539 636 54 249 275 319
Cuts at: 2633 2872 2879 2958 2989 4019 164
Size: 239 7 79 31 1030 2120
Fragments arranged by size:
2120 1030 636 539 319 289 275 249
239 108 79 54 31 7
BbeI G_GCGC'C
Cuts at: 347 596 922 2059 2108 2174 2332 3409 3657
Size: 249 326 1137 49 66 158 1077 248
Cuts at: 3657 347
Size: 2665
Fragments arranged by size:
2665 1137 1077 326 249 248 158 66
49
BbsI GAAGACnn'nnnn_
Cuts at: 309 1247 2309 309
Size: 938 1062 3975
Fragments arranged by size:
3975 1062 938
BbvCI CC'TCA_GC
Cuts at: 1645 2269 1645
Size: 624 5351
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1084 1118 1084
Size: 34 5941
BciVI GTATCCnnnnn_n'
Cuts at: 3873 4634 5975 3873
Size: 761 1341 3873
Fragments arranged by size:
3873 1341 761
BclI T'GATC_A
Cuts at: 3495 3495
Size: 5975
BglI GCCn_nnn'nGGC
Cuts at: 311 916 2179 2419 311
Size: 605 1263 240 3867
Fragments arranged by size:
3867 1263 605 240
BglII A'GATC_T
Cuts at: 3490 5527 3490
Size: 2037 3938
BlpI GC'TnA_GC
Cuts at: 1441 3087 1441
Size: 1646 4329
Bme1580I G_kGCm'C
Cuts at: 148 164 453 651 758 1093 1736 1790 1878
Size: 16 289 198 107 335 643 54 88
Cuts at: 1878 1985 2314 2582 2879 2945 2958 3600 3693
Size: 107 329 268 297 66 13 642 93
Cuts at: 3693 4749 148
Size: 1056 1374
Fragments arranged by size:
1374 1056 643 642 335 329 297 289
268 198 107 107 93 88 66 54
16 13
BmgBI CAC'GTC
Cuts at: 2178 2307 2550 2178
Size: 129 243 5603
Fragments arranged by size:
5603 243 129
BmrI ACTGGGnnnn_n'
Cuts at: 711 3187 3268 3603 5747 711
Size: 2476 81 335 2144 939
Fragments arranged by size:
2476 2144 939 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4342 4342
Size: 5975
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 820 987 1135 1237 1273 2518 2524 352
Size: 468 167 148 102 36 1245 6 3803
Fragments arranged by size:
3803 1245 468 167 148 102 36 6
Bpu10I CC'TnA_GC
Cuts at: 1645 2269 1645
Size: 624 5351
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 823 1486 3151 4030 4522 4820 760
Size: 63 663 1665 879 492 298 1915
Fragments arranged by size:
1915 1665 879 663 492 298 63
BsaI GGTCTCn'nnnn_
Cuts at: 2757 2817 5679 2757
Size: 60 2862 3053
Fragments arranged by size:
3053 2862 60
BsaAI yAC'GTr
Cuts at: 333 690 1088 3328 333
Size: 357 398 2240 2980
Fragments arranged by size:
2980 2240 398 357
BsaBI GATnn'nnATC
Cuts at: 6 1402 3489 5526 6
Size: 1396 2087 2037 455
Fragments arranged by size:
2087 2037 1396 455
BsaHI Gr'CG_yC
Cuts at: 314 344 593 919 1028 2056 2071 2105 2171
Size: 30 249 326 109 1028 15 34 66
Cuts at: 2171 2329 2590 3406 3654 4356 5852 314
Size: 158 261 816 248 702 1496 437
Fragments arranged by size:
1496 1028 816 702 437 326 261 249
248 158 109 66 34 30 15
BsaWI w'CCGG_w
Cuts at: 2862 3164 3685 4363 4637 4784 5195 2862
Size: 302 521 678 274 147 411 3642
Fragments arranged by size:
3642 678 521 411 302 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 517 547 1713 1743 2359 2389 517
Size: 30 1166 30 616 30 4103
Fragments arranged by size:
4103 1166 616 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 130 133 220 424 481 1327 1330 1369
Size: 3 3 87 204 57 846 3 39
Cuts at: 1369 1835 1840 1960 2221 127
Size: 466 5 120 261 3881
Fragments arranged by size:
3881 846 466 261 204 120 87 57
39 5 3 3 3
BseYI C'CCAG_C
Cuts at: 298 322 1124 1627 1789 1842 2151 2473 2607
Size: 24 802 503 162 53 309 322 134
Cuts at: 2607 2703 2845 2881 3400 4158 4735 5231 298
Size: 96 142 36 519 758 577 496 1042
Fragments arranged by size:
1042 802 758 577 519 503 496 322
309 162 142 134 96 53 36 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 730 1405 2908 338
Size: 392 675 1503 3405
Fragments arranged by size:
3405 1503 675 392
BsiEI CG_ry'CG
Cuts at: 104 231 306 2198 2253 2295 2423 2773 2823
Size: 127 75 1892 55 42 128 350 50
Cuts at: 2823 3517 3563 4771 104
Size: 694 46 1208 1308
Fragments arranged by size:
1892 1308 1208 694 350 128 127 75
55 50 46 42
BsiHKAI G_wGCw'C
Cuts at: 651 839 2989 3767 3957 4749 651
Size: 188 2150 778 190 792 1877
Fragments arranged by size:
2150 1877 792 778 190 188
BsmI GAATG_Cn'
Cuts at: 1126 1126
Size: 5975
BsmBI CGTCTCn'nnnn_
Cuts at: 940 940
Size: 5975
Bsp1286I G_dGCh'C
Cuts at: 148 164 453 561 651 758 839 1093 1100
Size: 16 289 108 90 107 81 254 7
Cuts at: 1100 1736 1790 1878 1985 2039 2314 2582 2633
Size: 636 54 88 107 54 275 268 51
Cuts at: 2633 2872 2879 2945 2958 2989 3600 3693 3767
Size: 239 7 66 13 31 611 93 74
Cuts at: 3767 3957 4019 4749 148
Size: 190 62 730 1374
Fragments arranged by size:
1374 730 636 611 289 275 268 254
239 190 108 107 107 93 90 88
81 74 66 62 54 54 51 31
16 13 7 7
BspEI T'CCGG_A
Cuts at: 2862 5195 2862
Size: 2333 3642
BspHI T'CATG_A
Cuts at: 5151 5151
Size: 5975
BspMI ACCTGCnnnn'nnnn_
Cuts at: 519 658 702 1433 3025 3239 3541 3922 519
Size: 139 44 731 1592 214 302 381 2572
Fragments arranged by size:
2572 1592 731 381 302 214 139 44
BsrBI CCG'CTC
Cuts at: 50 1812 2089 4267 4321 5805 5970 50
Size: 1762 277 2178 54 1484 165 55
Fragments arranged by size:
2178 1762 1484 277 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3887 3887
Size: 5975
BsrFI r'CCGG_y
Cuts at: 2248 2418 2582 2714 2772 3973 4154 4363 2248
Size: 170 164 132 58 1201 181 209 3860
Fragments arranged by size:
3860 1201 209 181 170 164 132 58
BssHII G'CGCG_C
Cuts at: 2188 2190 2188
Size: 2 5973
BssSI C'ACGA_G
Cuts at: 394 2344 2654 4246 4604 394
Size: 1950 310 1592 358 1765
Fragments arranged by size:
1950 1765 1592 358 310
BstAPI GCAn_nnn'nTGC
Cuts at: 3352 3352
Size: 5975
BstBI TT'CG_AA
Cuts at: 4336 4336
Size: 5975
BstXI CCAn_nnnn'nTGG
Cuts at: 2151 2487 2647 2901 2151
Size: 336 160 254 5225
Fragments arranged by size:
5225 336 254 160
BstYI r'GATC_y
Cuts at: 1 383 1597 3000 3467 3490 3825 4071 5083
Size: 382 1214 1403 467 23 335 246 1012
Cuts at: 5083 5169 5181 5527 1
Size: 86 12 346 449
Fragments arranged by size:
1403 1214 1012 467 449 382 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 586 586
Size: 5975
BtgI C'CryG_G
Cuts at: 107 155 173 359 1037 1140 1667 1725 2059
Size: 48 18 186 678 103 527 58 334
Cuts at: 2059 2260 2289 2574 2633 2640 2958 107
Size: 201 29 285 59 7 318 3124
Fragments arranged by size:
3124 678 527 334 318 285 201 186
103 59 58 48 29 18 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1896 3925 4106 4269 1896
Size: 2029 181 163 3602
Fragments arranged by size:
3602 2029 181 163
BtsI GCAGTG_nn'
Cuts at: 521 963 1695 1707 2023 2386 2896 3218 521
Size: 442 732 12 316 363 510 322 3278
Fragments arranged by size:
3278 732 510 442 363 322 316 12
DraI TTT'AAA
Cuts at: 2974 2974
Size: 5975
DrdI GACnn_nn'nnGTC
Cuts at: 3681 4539 3681
Size: 858 5117
EaeI y'GGCC_r
Cuts at: 131 303 1034 1147 2180 2195 2250 2292 2336
Size: 172 731 113 1033 15 55 42 44
Cuts at: 2336 2420 2770 2820 3560 3734 4125 4152 5497
Size: 84 350 50 740 174 391 27 1345
Cuts at: 5497 131
Size: 609
Fragments arranged by size:
1345 1033 740 731 609 391 350 174
172 113 84 55 50 44 42 27
15
EagI C'GGCC_G
Cuts at: 303 2195 2250 2292 2420 2770 2820 3560 303
Size: 1892 55 42 128 350 50 740 2718
Fragments arranged by size:
2718 1892 740 350 128 55 50 42
EarI CTCTTCn'nnn_
Cuts at: 949 2239 3998 4208 949
Size: 1290 1759 210 2716
Fragments arranged by size:
2716 1759 1290 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2601 4183 4493 4639 5785 2601
Size: 1582 310 146 1146 2791
Fragments arranged by size:
2791 1582 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2987 2987
Size: 5975
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 820 987 1135 1237 1273 1855 2518 2524
Size: 468 167 148 102 36 582 663 6
Cuts at: 2524 3800 4232 4979 5247 352
Size: 1276 432 747 268 1080
Fragments arranged by size:
1276 1080 747 663 582 468 432 268
167 148 102 36 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1114 1804 1114
Size: 690 5285
EcoO109I rG'GnC_Cy
Cuts at: 160 450 507 771 902 1008 1128 1733 1786
Size: 290 57 264 131 106 120 605 53
Cuts at: 1786 2265 2311 2520 2694 2876 2954 3114 160
Size: 479 46 209 174 182 78 160 3021
Fragments arranged by size:
3021 605 479 290 264 209 182 174
160 131 120 106 78 57 53 46
EcoRI G'AATT_C
Cuts at: 2979 2979
Size: 5975
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1497 3994 1497
Size: 2497 3478
FspI TGC'GCA
Cuts at: 3756 3756
Size: 5975
HaeII r_GCGC'y
Cuts at: 347 596 896 922 1347 2059 2108 2118 2131
Size: 249 300 26 425 712 49 10 13
Cuts at: 2131 2174 2220 2332 2608 3409 3657 4679 5658
Size: 43 46 112 276 801 248 1022 979
Cuts at: 5658 347
Size: 664
Fragments arranged by size:
1022 979 801 712 664 425 300 276
249 248 112 49 46 43 26 13
10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 213 245 763 795 2359 2391 3817 3847 3849
Size: 32 518 32 1564 32 1426 30 2
Cuts at: 3849 3879 213
Size: 30 2309
Fragments arranged by size:
2309 1564 1426 518 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 699 3014 699
Size: 2315 3660
KasI G'GCGC_C
Cuts at: 343 592 918 2055 2104 2170 2328 3405 3653
Size: 249 326 1137 49 66 158 1077 248
Cuts at: 3653 343
Size: 2665
Fragments arranged by size:
2665 1137 1077 326 249 248 158 66
49
KpnI G_GTAC'C
Cuts at: 2995 2995
Size: 5975
MluI A'CGCG_T
Cuts at: 2380 5408 2380
Size: 3028 2947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1600 2390 4646 4830 1600
Size: 790 2256 184 2745
Fragments arranged by size:
2745 2256 790 184
MscI TGG'CCA
Cuts at: 133 3736 133
Size: 3603 2372
MslI CAynn'nnrTG
Cuts at: 421 743 886 2344 2911 3964 4091 421
Size: 322 143 1458 567 1053 127 2305
Fragments arranged by size:
2305 1458 1053 567 322 143 127
MspA1I CmG'CkG
Cuts at: 157 175 302 352 361 372 523 1362 1440
Size: 18 127 50 9 11 151 839 78
Cuts at: 1440 1727 2214 2262 2576 2616 2635 3086 3400
Size: 287 487 48 314 40 19 451 314
Cuts at: 3400 3760 4773 5018 157
Size: 360 1013 245 1114
Fragments arranged by size:
1114 1013 839 487 451 360 314 314
287 245 151 127 78 50 48 40
19 18 11 9
NaeI GCC'GGC
Cuts at: 2250 2716 2774 4156 2250
Size: 466 58 1382 4069
Fragments arranged by size:
4069 1382 466 58
NarI GG'CG_CC
Cuts at: 344 593 919 2056 2105 2171 2329 3406 3654
Size: 249 326 1137 49 66 158 1077 248
Cuts at: 3654 344
Size: 2665
Fragments arranged by size:
2665 1137 1077 326 249 248 158 66
49
NcoI C'CATG_G
Cuts at: 107 2640 107
Size: 2533 3442
NgoMIV G'CCGG_C
Cuts at: 2248 2714 2772 4154 2248
Size: 466 58 1382 4069
Fragments arranged by size:
4069 1382 466 58
NotI GC'GGCC_GC
Cuts at: 303 303
Size: 5975
NspI r_CATG'y
Cuts at: 1269 2564 3028 3528 4435 1269
Size: 1295 464 500 907 2809
Fragments arranged by size:
2809 1295 907 500 464
PasI CC'CwG_GG
Cuts at: 2872 2872
Size: 5975
PciI A'CATG_T
Cuts at: 1265 4431 1265
Size: 3166 2809
PflMI CCAn_nnn'nTGG
Cuts at: 140 2599 140
Size: 2459 3516
PfoI T'CCnGG_A
Cuts at: 797 1112 1349 797
Size: 315 237 5423
Fragments arranged by size:
5423 315 237
PmeI GTTT'AAAC
Cuts at: 2974 2974
Size: 5975
PmlI CAC'GTG
Cuts at: 690 1088 690
Size: 398 5577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5114 5146 5114
Size: 32 5943
PpuMI rG'GwC_Cy
Cuts at: 507 771 902 1008 1128 2520 507
Size: 264 131 106 120 1392 3962
Fragments arranged by size:
3962 1392 264 131 120 106
PspOMI G'GGCC_C
Cuts at: 160 449 1732 1786 2310 2875 2954 160
Size: 289 1283 54 524 565 79 3181
Fragments arranged by size:
3181 1283 565 524 289 79 54
PstI C_TGCA'G
Cuts at: 404 566 1238 1805 3022 3707 404
Size: 162 672 567 1217 685 2672
Fragments arranged by size:
2672 1217 685 672 567 162
PvuI CG_AT'CG
Cuts at: 104 231 104
Size: 127 5848
PvuII CAG'CTG
Cuts at: 372 1362 1440 3400 3760 372
Size: 990 78 1960 360 2587
Fragments arranged by size:
2587 1960 990 360 78
RsrII CG'GwC_CG
Cuts at: 4170 4170
Size: 5975
SacI G_AGCT'C
Cuts at: 2989 2989
Size: 5975
SacII CC_GC'GG
Cuts at: 158 176 362 1728 2263 2577 2636 158
Size: 18 186 1366 535 314 59 3497
Fragments arranged by size:
3497 1366 535 314 186 59 18
SalI G'TCGA_C
Cuts at: 3012 3012
Size: 5975
SanDI GG'GwC_CC
Cuts at: 1128 1128
Size: 5975
SapI GCTCTTCn'nnn_
Cuts at: 3998 4208 3998
Size: 210 5765
SbfI CC_TGCA'GG
Cuts at: 3022 3022
Size: 5975
ScaI AGT'ACT
Cuts at: 5492 5492
Size: 5975
SfcI C'TryA_G
Cuts at: 33 400 562 1234 1801 3018 3703 4696 4887
Size: 367 162 672 567 1217 685 993 191
Cuts at: 4887 33
Size: 1121
Fragments arranged by size:
1217 1121 993 685 672 567 367 191
162
SfiI GGCCn_nnn'nGGCC
Cuts at: 2419 2419
Size: 5975
SfoI GGC'GCC
Cuts at: 345 594 920 2057 2106 2172 2330 3407 3655
Size: 249 326 1137 49 66 158 1077 248
Cuts at: 3655 345
Size: 2665
Fragments arranged by size:
2665 1137 1077 326 249 248 158 66
49
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5975
SgrAI Cr'CCGG_yG
Cuts at: 2582 2582
Size: 5975
SmaI CCC'GGG
Cuts at: 640 721 1459 2045 2997 640
Size: 81 738 586 952 3618
Fragments arranged by size:
3618 952 738 586 81
SmlI C'TyrA_G
Cuts at: 775 838 1501 3130 4045 4537 4799 775
Size: 63 663 1629 915 492 262 1951
Fragments arranged by size:
1951 1629 915 663 492 262 63
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5975
SphI G_CATG'C
Cuts at: 2564 3028 3528 2564
Size: 464 500 5011
Fragments arranged by size:
5011 500 464
StuI AGG'CCT
Cuts at: 1570 1570
Size: 5975
StyI C'CwwG_G
Cuts at: 107 134 1031 1286 2640 3109 3463 107
Size: 27 897 255 1354 469 354 2619
Fragments arranged by size:
2619 1354 897 469 354 255 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1923 2475 2560 3558 3691 4150 4359 1923
Size: 552 85 998 133 459 209 3539
Fragments arranged by size:
3539 998 552 459 209 133 85
TatI w'GTAC_w
Cuts at: 5490 5490
Size: 5975
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 517 549 1713 1745 5492 5524 517
Size: 32 1164 32 3747 32 968
Fragments arranged by size:
3747 1164 968 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2004 2073 3772 2004
Size: 69 1699 4207
Fragments arranged by size:
4207 1699 69
XbaI T'CTAG_A
Cuts at: 3006 3006
Size: 5975
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 306 330 2487 3470 306
Size: 24 2157 983 2811
Fragments arranged by size:
2811 2157 983 24
XmaI C'CCGG_G
Cuts at: 638 719 1457 2043 2995 638
Size: 81 738 586 952 3618
Fragments arranged by size:
3618 952 738 586 81
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AloI AscI AvrII BaeI BfrBI BmtI
BsiWI BsrGI BstEII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NruI
NsiI PacI PshAI PsiI PspXI PsrI SexAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI