Restriction map for
pF1KE3836
(Circular) (Six-base) MAPSORT of: pF1KE3836.seq Check: 6344 from: 1 to: 16745
pF1KE3836 16745 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 511 592 2797 2920 3040 3775 4674 4846 7663
Size: 81 2205 123 120 735 899 172 2817
Cuts at: 7663 11614 11692 11907 12906 12958 13201 511
Size: 3951 78 215 999 52 243 4055
Fragments arranged by size:
4055 3951 2817 2205 999 899 735 243
215 172 123 120 81 78 52
AatII G_ACGT'C
Cuts at: 12398 12398
Size: 16745
AccI GT'mk_AC
Cuts at: 692 766 1193 1250 2402 4160 4580 9839 13783
Size: 74 427 57 1152 1758 420 5259 3944
Cuts at: 13783 692
Size: 3654
Fragments arranged by size:
5259 3944 3654 1758 1152 427 420 74
57
Acc65I G'GTAC_C
Cuts at: 9578 13761 9578
Size: 4183 12562
AclI AA'CG_TT
Cuts at: 16585 16585
Size: 16745
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 795 1620 1918 5304 6692 6861 7710 9046
Size: 281 825 298 3386 1388 169 849 1336
Cuts at: 9046 11998 12369 13329 14570 15002 15749 16017 514
Size: 2952 371 960 1241 432 747 268 1242
Fragments arranged by size:
3386 2952 1388 1336 1242 1241 960 849
825 747 432 371 298 281 268 169
AfeI AGC'GCT
Cuts at: 2716 8479 9091 11365 2716
Size: 5763 612 2274 8096
Fragments arranged by size:
8096 5763 2274 612
AflII C'TTAA_G
Cuts at: 9262 9262
Size: 16745
AflIII A'CryG_T
Cuts at: 1045 2968 6398 6578 8248 8462 9043 9254 11393
Size: 1923 3430 180 1670 214 581 211 2139
Cuts at: 11393 12020 14097 15201 16178 1045
Size: 627 2077 1104 977 1612
Fragments arranged by size:
3430 2139 2077 1923 1670 1612 1104 977
627 581 214 211 180
AgeI A'CCGG_T
Cuts at: 2822 9212 15133 2822
Size: 6390 5921 4434
Fragments arranged by size:
6390 5921 4434
AhdI GACnn_n'nnGTC
Cuts at: 1656 4467 1656
Size: 2811 13934
AleI CACnn'nnGTG
Cuts at: 476 1471 2664 3560 5050 5593 6577 6940 8461
Size: 995 1193 896 1490 543 984 363 1521
Cuts at: 8461 8627 10049 10096 11056 12652 476
Size: 166 1422 47 960 1596 4569
Fragments arranged by size:
4569 1596 1521 1490 1422 1193 995 984
960 896 543 363 166 47
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3355 3578 4568 8556 8892 9827 10067 11005 11074
Size: 223 990 3988 336 935 240 938 69
Cuts at: 11074 12670 12938 13303 3355
Size: 1596 268 365 6797
Fragments arranged by size:
6797 3988 1596 990 938 935 365 336
268 240 223 69
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3514 3546 3514
Size: 32 16713
AlwNI CAG_nnn'CTG
Cuts at: 2702 5208 6412 6612 7363 7581 7976 8361 8838
Size: 2506 1204 200 751 218 395 385 477
Cuts at: 8838 8960 9273 11480 11715 12950 13145 14050 15617
Size: 122 313 2207 235 1235 195 905 1567
Cuts at: 15617 2702
Size: 3830
Fragments arranged by size:
3830 2506 2207 1567 1235 1204 905 751
477 395 385 313 235 218 200 195
122
ApaI G_GGCC'C
Cuts at: 425 500 759 1976 3755 4750 5338 12901 13325
Size: 75 259 1217 1779 995 588 7563 424
Cuts at: 13325 13711 425
Size: 386 3459
Fragments arranged by size:
7563 3459 1779 1217 995 588 424 386
259 75
ApaLI G'TGCA_C
Cuts at: 4384 5724 15515 4384
Size: 1340 9791 5614
Fragments arranged by size:
9791 5614 1340
ApoI r'AATT_y
Cuts at: 5816 7668 9748 13749 16098 16354 5816
Size: 1852 2080 4001 2349 256 6207
Fragments arranged by size:
6207 4001 2349 2080 1852 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 16745
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 16745
AvaI C'yCGr_G
Cuts at: 1469 1520 1790 3193 4144 4295 5559 6137 7197
Size: 51 270 1403 951 151 1264 578 1060
Cuts at: 7197 8473 8659 10033 10453 10511 11167 11538 12036
Size: 1276 186 1374 420 58 656 371 498
Cuts at: 12036 12096 13765 1469
Size: 60 1669 4449
Fragments arranged by size:
4449 1669 1403 1374 1276 1264 1060 951
656 578 498 420 371 270 186 151
60 58 51
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 7221 7254 7221
Size: 33 16712
BamHI G'GATC_C
Cuts at: 2073 4290 4304 5826 13770 2073
Size: 2217 14 1522 7944 5048
Fragments arranged by size:
7944 5048 2217 1522 14
BanI G'GyrC_C
Cuts at: 580 1486 2240 2515 3702 3984 4373 4938 5644
Size: 906 754 275 1187 282 389 565 706
Cuts at: 5644 6571 6602 6844 6856 8131 8455 9578 9892
Size: 927 31 242 12 1275 324 1123 314
Cuts at: 9892 11246 11602 11872 12838 12878 13761 14175 14423
Size: 1354 356 270 966 40 883 414 248
Cuts at: 14423 14458 580
Size: 35 2867
Fragments arranged by size:
2867 1354 1275 1187 1123 966 927 906
883 754 706 565 414 389 356 324
314 282 275 270 248 242 40 35
31 12
BanII G_rGCy'C
Cuts at: 425 440 500 759 1797 1976 3650 3755 4101
Size: 15 60 259 1038 179 1674 105 346
Cuts at: 4101 4424 4750 5338 5566 6958 8725 9548 9555
Size: 323 326 588 228 1392 1767 823 7
Cuts at: 9555 9879 10205 10565 10688 11306 12116 12598 12901
Size: 324 326 360 123 618 810 482 303
Cuts at: 12901 13325 13711 13759 14789 425
Size: 424 386 48 1030 2381
Fragments arranged by size:
2381 1767 1674 1392 1038 1030 823 810
618 588 482 424 386 360 346 326
326 324 323 303 259 228 179 123
105 60 48 15 7
BbeI G_GCGC'C
Cuts at: 2244 4377 8135 11250 14179 14427 2244
Size: 2133 3758 3115 2929 248 4562
Fragments arranged by size:
4562 3758 3115 2929 2133 248
BbsI GAAGACnn'nnnn_
Cuts at: 1420 5984 6540 6680 7816 9939 11677 12435 13335
Size: 4564 556 140 1136 2123 1738 758 900
Cuts at: 13335 1420
Size: 4830
Fragments arranged by size:
4830 4564 2123 1738 1136 900 758 556
140
BbvCI CC'TCA_GC
Cuts at: 2597 4024 8864 9409 2597
Size: 1427 4840 545 9933
Fragments arranged by size:
9933 4840 1427 545
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1170 1204 2792 2826 3987 4021 7249
Size: 34 1046 34 1588 34 1161 34 3228
Cuts at: 7249 7283 9636 9670 11158 11192 11379 11413 11982
Size: 34 2353 34 1488 34 187 34 569
Cuts at: 11982 12016 90
Size: 34 4819
Fragments arranged by size:
4819 3228 2353 1588 1488 1161 1046 569
187 34 34 34 34 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 634 6206 6751 14643 15404 16745 634
Size: 5572 545 7892 761 1341 634
Fragments arranged by size:
7892 5572 1341 761 634 545
BclI T'GATC_A
Cuts at: 5514 14265 5514
Size: 8751 7994
BfrBI ATG'CAT
Cuts at: 3332 3332
Size: 16745
BglI GCCn_nnn'nGGC
Cuts at: 902 1631 1712 2214 2675 3737 4419 5333 5342
Size: 729 81 502 461 1062 682 914 9
Cuts at: 5342 5465 7628 9626 9764 11540 12887 12995 902
Size: 123 2163 1998 138 1776 1347 108 4652
Fragments arranged by size:
4652 2163 1998 1776 1347 1062 914 729
682 502 461 138 123 108 81 9
BglII A'GATC_T
Cuts at: 4067 14260 16297 4067
Size: 10193 2037 4515
Fragments arranged by size:
10193 4515 2037
BlpI GC'TnA_GC
Cuts at: 2218 9787 12592 13857 2218
Size: 7569 2805 1265 5106
Fragments arranged by size:
7569 5106 2805 1265
Bme1580I G_kGCm'C
Cuts at: 425 500 759 885 1491 1976 3755 3989 4388
Size: 75 259 126 606 485 1779 234 399
Cuts at: 4388 4750 4943 5338 5539 5647 5728 6506 6657
Size: 362 193 395 201 108 81 778 151
Cuts at: 6657 6859 9977 10046 10757 11181 11194 11558 11875
Size: 202 3118 69 711 424 13 364 317
Cuts at: 11875 12665 12841 12883 12901 13306 13325 13711 14370
Size: 790 176 42 18 405 19 386 659
Cuts at: 14370 14463 15519 425
Size: 93 1056 1651
Fragments arranged by size:
3118 1779 1651 1056 790 778 711 659
606 485 424 405 399 395 386 364
362 317 259 234 202 201 193 176
151 126 108 93 81 75 69 42
19 18 13
BmgBI CAC'GTC
Cuts at: 2969 3414 4053 4873 5007 5699 5969 5982 8249
Size: 445 639 820 134 692 270 13 2267
Cuts at: 8249 8426 9044 9972 10341 12513 13341 2969
Size: 177 618 928 369 2172 828 6373
Fragments arranged by size:
6373 2267 2172 928 828 820 692 639
618 445 369 270 177 134 13
BmrI ACTGGGnnnn_n'
Cuts at: 1263 2115 2259 2658 3156 3419 4194 4332 5115
Size: 852 144 399 498 263 775 138 783
Cuts at: 5115 5851 5928 7425 8313 8681 9447 9606 9729
Size: 736 77 1497 888 368 766 159 123
Cuts at: 9729 9861 10346 12015 12174 12297 12593 12972 13957
Size: 132 485 1669 159 123 296 379 985
Cuts at: 13957 14038 14373 16517 1263
Size: 81 335 2144 1491
Fragments arranged by size:
2144 1669 1497 1491 985 888 852 783
775 766 736 498 485 399 379 368
335 296 263 159 159 144 138 132
123 123 81 77
BmtI G_CTAG'C
Cuts at: 10118 10118
Size: 16745
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3525 4371 4416 8022 8112 15112 3525
Size: 846 45 3606 90 7000 5158
Fragments arranged by size:
7000 5158 3606 846 90 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2023 2164 3108 3363 4459 5230 5455 6370 6456
Size: 141 944 255 1096 771 225 915 86
Cuts at: 6456 6909 7115 7624 7953 2023
Size: 453 206 509 329 10815
Fragments arranged by size:
10815 1096 944 915 771 509 453 329
255 225 206 141 86
Bpu10I CC'TnA_GC
Cuts at: 2327 2597 4024 4613 5935 6797 8864 9409 12496
Size: 270 1427 589 1322 862 2067 545 3087
Cuts at: 12496 13460 2327
Size: 964 5612
Fragments arranged by size:
5612 3087 2067 1427 1322 964 862 589
545 270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 6718 8968 13921 14800 15292 15590 859
Size: 5859 2250 4953 879 492 298 2014
Fragments arranged by size:
5859 4953 2250 2014 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 820 1807 1882 4387 5115 6218 7069 8281 10889
Size: 987 75 2505 728 1103 851 1212 2608
Cuts at: 10889 16449 820
Size: 5560 1116
Fragments arranged by size:
5560 2608 2505 1212 1116 1103 987 851
728 75
BsaAI yAC'GTr
Cuts at: 743 951 2652 6108 7452 8463 12872 14098 743
Size: 208 1701 3456 1344 1011 4409 1226 3390
Fragments arranged by size:
4409 3456 3390 1701 1344 1226 1011 208
BsaBI GATnn'nnATC
Cuts at: 6 964 1265 4882 14259 16296 6
Size: 958 301 3617 9377 2037 455
Fragments arranged by size:
9377 3617 2037 958 455 301
BsaHI Gr'CG_yC
Cuts at: 173 1512 2241 2624 4374 4463 6524 7292 7745
Size: 1339 729 383 1750 89 2061 768 453
Cuts at: 7745 8003 8132 9728 11012 11247 12296 12395 14176
Size: 258 129 1596 1284 235 1049 99 1781
Cuts at: 14176 14424 15126 16622 173
Size: 248 702 1496 296
Fragments arranged by size:
2061 1781 1750 1596 1496 1339 1284 1049
768 729 702 453 383 296 258 248
235 129 99 89
BsaWI w'CCGG_w
Cuts at: 2303 2822 4238 4781 5744 9212 13934 14455 15133
Size: 519 1416 543 963 3468 4722 521 678
Cuts at: 15133 15407 15554 15965 2303
Size: 274 147 411 3083
Fragments arranged by size:
4722 3468 3083 1416 963 678 543 521
519 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2137 2167 2382 2412 2914 2944 4681 4711 4912
Size: 30 215 30 502 30 1737 30 201
Cuts at: 4912 4942 5095 5125 6151 6181 6907 6937 6940
Size: 30 153 30 1026 30 726 30 3
Cuts at: 6940 6970 7193 7223 7777 7807 11335 11365 2137
Size: 30 223 30 554 30 3528 30 7517
Fragments arranged by size:
7517 3528 1737 1026 726 554 502 223
215 201 153 30 30 30 30 30
30 30 30 30 30 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1542 2125 3532 8427 9898 11053 11185 11364 11367
Size: 583 1407 4895 1471 1155 132 179 3
Cuts at: 11367 11440 13348 1542
Size: 73 1908 4939
Fragments arranged by size:
4939 4895 1908 1471 1407 1155 583 179
132 73 3
BseYI C'CCAG_C
Cuts at: 314 836 1406 2236 2498 2766 3747 3860 4369
Size: 522 570 830 262 268 981 113 509
Cuts at: 4369 4667 5463 5681 6986 7174 7490 8113 8117
Size: 298 796 218 1305 188 316 623 4
Cuts at: 8117 10632 11612 12815 12911 13222 14170 14928 15505
Size: 2515 980 1203 96 311 948 758 577
Cuts at: 15505 16001 314
Size: 496 1058
Fragments arranged by size:
2515 1305 1203 1058 981 980 948 830
796 758 623 577 570 522 509 496
316 311 298 268 262 218 188 113
96 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 3243 5221 6799 7453 7845 8537 8657 8781
Size: 2315 1978 1578 654 392 692 120 124
Cuts at: 8781 8862 9084 9406 9589 9907 10092 10248 10557
Size: 81 222 322 183 318 185 156 309
Cuts at: 10557 10800 10917 12247 12597 12936 13080 13158 13185
Size: 243 117 1330 350 339 144 78 27
Cuts at: 13185 928
Size: 4488
Fragments arranged by size:
4488 2315 1978 1578 1330 692 654 392
350 339 322 318 309 243 222 185
183 156 144 124 120 117 81 78
27
BsiEI CG_ry'CG
Cuts at: 104 2271 8568 8628 9039 9621 10530 10761 10863
Size: 2167 6297 60 411 582 909 231 102
Cuts at: 10863 13011 14287 14333 15541 104
Size: 2148 1276 46 1208 1308
Fragments arranged by size:
6297 2167 2148 1308 1276 1208 909 582
411 231 102 60 46
BsiHKAI G_wGCw'C
Cuts at: 440 571 787 920 1343 1760 3200 3650 3664
Size: 131 216 133 423 417 1440 450 14
Cuts at: 3664 3847 4271 4388 5728 5941 6803 7400 7686
Size: 183 424 117 1340 213 862 597 286
Cuts at: 7686 8075 8725 8813 10252 10489 12824 13033 13340
Size: 389 650 88 1439 237 2335 209 307
Cuts at: 13340 13759 14537 14727 15519 440
Size: 419 778 190 792 1666
Fragments arranged by size:
2335 1666 1440 1439 1340 862 792 778
650 597 450 424 423 419 417 389
307 286 237 216 213 209 190 183
133 131 117 88 14
BsmI GAATG_Cn'
Cuts at: 11785 11785
Size: 16745
BsmBI CGTCTCn'nnnn_
Cuts at: 2272 5070 5698 12181 2272
Size: 2798 628 6483 6836
Fragments arranged by size:
6836 6483 2798 628
Bsp1286I G_dGCh'C
Cuts at: 425 440 500 571 759 787 885 920 1343
Size: 15 60 71 188 28 98 35 423
Cuts at: 1343 1491 1760 1797 1976 3200 3650 3664 3755
Size: 148 269 37 179 1224 450 14 91
Cuts at: 3755 3847 3989 4101 4271 4388 4424 4750 4943
Size: 92 142 112 170 117 36 326 193
Cuts at: 4943 5338 5539 5566 5647 5728 5941 6506 6657
Size: 395 201 27 81 81 213 565 151
Cuts at: 6657 6803 6859 6958 7400 7686 8075 8725 8813
Size: 146 56 99 442 286 389 650 88
Cuts at: 8813 9548 9555 9879 9977 10046 10205 10252 10489
Size: 735 7 324 98 69 159 47 237
Cuts at: 10489 10565 10688 10757 11181 11194 11306 11558 11875
Size: 76 123 69 424 13 112 252 317
Cuts at: 11875 12116 12598 12665 12824 12841 12883 12901 13033
Size: 241 482 67 159 17 42 18 132
Cuts at: 13033 13306 13325 13340 13711 13759 14370 14463 14537
Size: 273 19 15 371 48 611 93 74
Cuts at: 14537 14727 14789 15519 425
Size: 190 62 730 1651
Fragments arranged by size:
1651 1224 735 730 650 611 565 482
450 442 424 423 395 389 371 326
324 317 286 273 269 252 241 237
213 201 193 190 188 179 170 159
159 151 148 146 142 132 123 117
112 112 99 98 98 93 92 91
88 81 81 76 74 71 69 69
67 62 60 56 48 47 42 37
36 35 28 27 19 18 17 15
15 14 13 7
BspEI T'CCGG_A
Cuts at: 4238 5744 15965 4238
Size: 1506 10221 5018
Fragments arranged by size:
10221 5018 1506
BspHI T'CATG_A
Cuts at: 1781 4811 11666 15921 1781
Size: 3030 6855 4255 2605
Fragments arranged by size:
6855 4255 3030 2605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 511 556 592 1624 2797 2920 3040 3064 3214
Size: 45 36 1032 1173 123 120 24 150
Cuts at: 3214 3775 4674 4688 4846 6716 7663 7744 7963
Size: 561 899 14 158 1870 947 81 219
Cuts at: 7963 8686 9230 9378 9947 10471 11074 11614 11692
Size: 723 544 148 569 524 603 540 78
Cuts at: 11692 11907 12719 12906 12958 13201 13243 13795 14009
Size: 215 812 187 52 243 42 552 214
Cuts at: 14009 14311 14692 511
Size: 302 381 2564
Fragments arranged by size:
2564 1870 1173 1032 947 899 812 723
603 569 561 552 544 540 524 381
302 243 219 215 214 187 158 150
148 123 120 81 78 52 45 42
36 24 14
BsrBI CCG'CTC
Cuts at: 50 665 1884 2463 2999 5714 6210 7314 7663
Size: 615 1219 579 536 2715 496 1104 349
Cuts at: 7663 8091 10973 11537 12279 15037 15091 16575 16740
Size: 428 2882 564 742 2758 54 1484 165
Cuts at: 16740 50
Size: 55
Fragments arranged by size:
2882 2758 2715 1484 1219 1104 742 615
579 564 536 496 428 349 165 55
54
BsrDI GCAATG_nn'
Cuts at: 390 1054 1088 6014 7836 8826 9387 10413 12837
Size: 664 34 4926 1822 990 561 1026 2424
Cuts at: 12837 13260 14657 390
Size: 423 1397 2478
Fragments arranged by size:
4926 2478 2424 1822 1397 1026 990 664
561 423 34
BsrFI r'CCGG_y
Cuts at: 695 1706 1816 2822 3133 3314 4255 4396 4630
Size: 1011 110 1006 311 181 941 141 234
Cuts at: 4630 5731 6514 6560 6700 9212 9926 10229 11201
Size: 1101 783 46 140 2512 714 303 972
Cuts at: 11201 11930 12875 13022 14743 14924 15133 695
Size: 729 945 147 1721 181 209 2307
Fragments arranged by size:
2512 2307 1721 1101 1011 1006 972 945
941 783 729 714 311 303 234 209
181 181 147 141 140 110 46
BsrGI T'GTAC_A
Cuts at: 8546 11717 8546
Size: 3171 13574
BssSI C'ACGA_G
Cuts at: 1690 3273 4531 15016 15374 1690
Size: 1583 1258 10485 358 3061
Fragments arranged by size:
10485 3061 1583 1258 358
BstAPI GCAn_nnn'nTGC
Cuts at: 548 2558 8426 10019 12572 14122 548
Size: 2010 5868 1593 2553 1550 3171
Fragments arranged by size:
5868 3171 2553 2010 1593 1550
BstBI TT'CG_AA
Cuts at: 15106 15106
Size: 16745
BstEII G'GTnAC_C
Cuts at: 5985 9601 10458 5985
Size: 3616 857 12272
Fragments arranged by size:
12272 3616 857
BstXI CCAn_nnnn'nTGG
Cuts at: 804 2344 4011 4251 5481 7251 9555 10640 11257
Size: 1540 1667 240 1230 1770 2304 1085 617
Cuts at: 11257 12129 12255 12460 13503 804
Size: 872 126 205 1043 4046
Fragments arranged by size:
4046 2304 1770 1667 1540 1230 1085 1043
872 617 240 205 126
BstYI r'GATC_y
Cuts at: 1 274 1016 1139 2073 2480 3773 4067 4290
Size: 273 742 123 934 407 1293 294 223
Cuts at: 4290 4304 4883 5826 7898 11738 12449 13770 14237
Size: 14 579 943 2072 3840 711 1321 467
Cuts at: 14237 14260 14595 14841 15853 15939 15951 16297 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
3840 2072 1321 1293 1012 943 934 742
711 579 467 449 407 346 335 294
273 246 223 123 86 23 14 12
Bsu36I CC'TnA_GG
Cuts at: 13712 13712
Size: 16745
BtgI C'CryG_G
Cuts at: 803 1106 1931 2358 4074 4427 4646 4709 5088
Size: 303 825 427 1716 353 219 63 379
Cuts at: 5088 5220 5339 5567 6630 6657 6959 6981 7108
Size: 132 119 228 1063 27 302 22 127
Cuts at: 7108 7499 7514 9105 9147 9932 12260 12884 13650
Size: 391 15 1591 42 785 2328 624 766
Cuts at: 13650 803
Size: 3898
Fragments arranged by size:
3898 2328 1716 1591 1063 825 785 766
624 427 391 379 353 303 302 228
219 132 127 119 63 42 27 22
15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 333 3224 3368 3398 3990 6329 6801 7494 8323
Size: 2891 144 30 592 2339 472 693 829
Cuts at: 8323 8390 8514 10097 10301 10854 10904 11141 11270
Size: 67 124 1583 204 553 50 237 129
Cuts at: 11270 11706 11979 12211 14695 14876 15039 333
Size: 436 273 232 2484 181 163 2039
Fragments arranged by size:
2891 2484 2339 2039 1583 829 693 592
553 472 436 273 237 232 204 181
163 144 129 124 67 50 30
BtsI GCAGTG_nn'
Cuts at: 426 1653 2403 2463 4562 5201 5664 6026 7436
Size: 1227 750 60 2099 639 463 362 1410
Cuts at: 7436 7752 7828 8031 8292 8362 8793 8853 8869
Size: 316 76 203 261 70 431 60 16
Cuts at: 8869 10560 10774 10977 11325 12721 13033 13097 13245
Size: 1691 214 203 348 1396 312 64 148
Cuts at: 13245 13988 426
Size: 743 3183
Fragments arranged by size:
3183 2099 1691 1410 1396 1227 750 743
639 463 431 362 348 316 312 261
214 203 203 148 76 70 64 60
60 16
ClaI AT'CG_AT
Cuts at: 1007 3953 5444 1007
Size: 2946 1491 12308
Fragments arranged by size:
12308 2946 1491
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1973 2008 2847 2882 3093 3128 7548 7583 8124
Size: 35 839 35 211 35 4420 35 541
Cuts at: 8124 8159 8937 8972 9984 10019 13161 13196 1973
Size: 35 778 35 1012 35 3142 35 5522
Fragments arranged by size:
5522 4420 3142 1012 839 778 541 211
35 35 35 35 35 35 35 35
DraI TTT'AAA
Cuts at: 13744 13744
Size: 16745
DraIII CAC_nnn'GTG
Cuts at: 951 1917 7041 8613 9783 11102 11553 951
Size: 966 5124 1572 1170 1319 451 6143
Fragments arranged by size:
6143 5124 1572 1319 1170 966 451
DrdI GACnn_nn'nnGTC
Cuts at: 1048 2182 3976 6544 9943 10339 11803 11950 14451
Size: 1134 1794 2568 3399 396 1464 147 2501
Cuts at: 14451 15309 1048
Size: 858 2484
Fragments arranged by size:
3399 2568 2501 2484 1794 1464 1134 858
396 147
EaeI y'GGCC_r
Cuts at: 737 962 1592 1708 1857 1928 1994 2564 2943
Size: 225 630 116 149 71 66 570 379
Cuts at: 2943 4136 4217 4253 4506 4632 5285 5325 5483
Size: 1193 81 36 253 126 653 40 158
Cuts at: 5483 5751 6000 6590 7253 7517 8883 9036 9618
Size: 268 249 590 663 264 1366 153 582
Cuts at: 9618 10758 10860 10974 11559 11637 11757 11841 12011
Size: 1140 102 114 585 78 120 84 170
Cuts at: 12011 12257 12462 13209 13242 13418 13670 14330 14504
Size: 246 205 747 33 176 252 660 174
Cuts at: 14504 14895 14922 16267 737
Size: 391 27 1345 1215
Fragments arranged by size:
1366 1345 1215 1193 1140 747 663 660
653 630 590 585 582 570 391 379
268 264 253 252 249 246 225 205
176 174 170 158 153 149 126 120
116 114 102 84 81 78 71 66
40 36 33 27
EagI C'GGCC_G
Cuts at: 9036 9618 10758 10860 14330 9036
Size: 582 1140 102 3470 11451
Fragments arranged by size:
11451 3470 1140 582 102
EarI CTCTTCn'nnn_
Cuts at: 2377 3385 5323 8080 10688 10802 11285 14768 14978
Size: 1008 1938 2757 2608 114 483 3483 210
Cuts at: 14978 2377
Size: 4144
Fragments arranged by size:
4144 3483 2757 2608 1938 1008 483 210
114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 8041 13556 14953 15263 15409 16555 8041
Size: 5515 1397 310 146 1146 8231
Fragments arranged by size:
8231 5515 1397 1146 310 146
EcoICRI GAG'CTC
Cuts at: 438 3648 8723 13757 438
Size: 3210 5075 5034 3426
Fragments arranged by size:
5075 5034 3426 3210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 795 1620 1918 2023 2164 3108 3363 4459
Size: 281 825 298 105 141 944 255 1096
Cuts at: 4459 5230 5304 5455 6370 6456 6692 6861 6909
Size: 771 74 151 915 86 236 169 48
Cuts at: 6909 7115 7624 7710 7953 9046 11998 12369 13329
Size: 206 509 86 243 1093 2952 371 960
Cuts at: 13329 14570 15002 15749 16017 514
Size: 1241 432 747 268 1242
Fragments arranged by size:
2952 1242 1241 1096 1093 960 944 915
825 771 747 509 432 371 298 281
268 255 243 236 206 169 151 141
105 86 86 74 48
EcoNI CCTnn'n_nnAGG
Cuts at: 3710 9826 3710
Size: 6116 10629
EcoO109I rG'GnC_Cy
Cuts at: 285 421 422 755 1746 2115 2323 2763 3278
Size: 136 1 333 991 369 208 440 515
Cuts at: 3278 3751 3864 4149 4232 4746 4747 5205 5334
Size: 473 113 285 83 514 1 458 129
Cuts at: 5334 5335 5867 6792 7061 7578 7952 8979 9270
Size: 1 532 925 269 517 374 1027 291
Cuts at: 9270 9390 9506 11712 12492 12898 13157 13321 13322
Size: 120 116 2206 780 406 259 164 1
Cuts at: 13322 13707 13708 13728 13884 285
Size: 385 1 20 156 3146
Fragments arranged by size:
3146 2206 1027 991 925 780 532 517
515 514 473 458 440 406 385 374
369 333 291 285 269 259 208 164
156 136 129 120 116 113 83 20
1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 5816 7668 13749 5816
Size: 1852 6081 8812
Fragments arranged by size:
8812 6081 1852
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5049 7299 9009 14764 5049
Size: 2250 1710 5755 7030
Fragments arranged by size:
7030 5755 2250 1710
FseI GG_CCGG'CC
Cuts at: 1710 1710
Size: 16745
FspI TGC'GCA
Cuts at: 2455 3997 9226 11506 14526 2455
Size: 1542 5229 2280 3020 4674
Fragments arranged by size:
5229 4674 3020 2280 1542
FspAI rTGC'GCAy
Cuts at: 11506 11506
Size: 16745
HaeII r_GCGC'y
Cuts at: 2244 2718 3351 4037 4377 7195 7839 8135 8229
Size: 474 633 686 340 2818 644 296 94
Cuts at: 8229 8481 8715 8856 9093 10881 11001 11250 11367
Size: 252 234 141 237 1788 120 249 117
Cuts at: 11367 14179 14427 15449 16428 2244
Size: 2812 248 1022 979 2561
Fragments arranged by size:
2818 2812 2561 1788 1022 979 686 644
633 474 340 296 252 249 248 237
234 141 120 117 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 553 585 649 681 1642 1674 2370 2402 2958
Size: 32 64 32 961 32 696 32 556
Cuts at: 2958 2990 3317 3349 3646 3678 4453 4485 4525
Size: 32 327 32 297 32 775 32 40
Cuts at: 4525 4557 5095 5127 5506 5538 6667 6699 6907
Size: 32 538 32 379 32 1129 32 208
Cuts at: 6907 6939 7193 7225 7765 7797 14587 14617 14619
Size: 32 254 32 540 32 6790 30 2
Cuts at: 14619 14649 553
Size: 30 2649
Fragments arranged by size:
6790 2649 1129 961 775 696 556 540
538 379 327 297 254 208 64 40
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1194 5664 6249 7611 9996 12480 12960 13170 13784
Size: 4470 585 1362 2385 2484 480 210 614
Cuts at: 13784 1194
Size: 4155
Fragments arranged by size:
4470 4155 2484 2385 1362 614 585 480
210
HindIII A'AGCT_T
Cuts at: 12301 12301
Size: 16745
KasI G'GCGC_C
Cuts at: 2240 4373 8131 11246 14175 14423 2240
Size: 2133 3758 3115 2929 248 4562
Fragments arranged by size:
4562 3758 3115 2929 2133 248
KpnI G_GTAC'C
Cuts at: 9582 13765 9582
Size: 4183 12562
MluI A'CGCG_T
Cuts at: 16178 16178
Size: 16745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2591 3953 5504 6731 6927 7871 9272 10127 10621
Size: 1362 1551 1227 196 944 1401 855 494
Cuts at: 10621 11945 12694 15416 15600 2591
Size: 1324 749 2722 184 3736
Fragments arranged by size:
3736 2722 1551 1401 1362 1324 1227 944
855 749 494 196 184
MscI TGG'CCA
Cuts at: 739 964 1594 1996 2566 4138 4219 4508 5287
Size: 225 630 402 570 1572 81 289 779
Cuts at: 5287 5327 5485 5753 6592 7255 7519 12464 13672
Size: 40 158 268 839 663 264 4945 1208
Cuts at: 13672 14506 739
Size: 834 2978
Fragments arranged by size:
4945 2978 1572 1208 839 834 779 663
630 570 402 289 268 264 225 158
81 40
MslI CAynn'nnrTG
Cuts at: 476 802 1471 1538 1732 2664 3299 3560 4516
Size: 326 669 67 194 932 635 261 956
Cuts at: 4516 5050 5281 5593 6019 6280 6397 6577 6940
Size: 534 231 312 426 261 117 180 363
Cuts at: 6940 7087 7340 7513 8236 8461 8627 8930 9487
Size: 147 253 173 723 225 166 303 557
Cuts at: 9487 9628 10049 10096 10579 11056 12127 12652 13501
Size: 141 421 47 483 477 1071 525 849
Cuts at: 13501 14734 14861 476
Size: 1233 127 2360
Fragments arranged by size:
2360 1233 1071 956 932 849 723 669
635 557 534 525 483 477 426 421
363 326 312 303 261 261 253 231
225 194 180 173 166 147 141 127
117 67 47
MspA1I CmG'CkG
Cuts at: 1622 2702 2840 2948 3179 4076 5411 5463 5631
Size: 1080 138 108 231 897 1335 52 168
Cuts at: 5631 6263 6612 6639 7437 7535 7802 7940 7976
Size: 632 349 27 798 98 267 138 36
Cuts at: 7976 8117 8536 8699 9005 9149 9167 10382 10403
Size: 141 419 163 306 144 18 1215 21
Cuts at: 10403 10607 10625 10733 10748 10763 10865 10943 11112
Size: 204 18 108 15 15 102 78 169
Cuts at: 11112 11459 11651 12734 12770 13013 13094 13145 13226
Size: 347 192 1083 36 243 81 51 81
Cuts at: 13226 13856 14170 14530 15543 15788 1622
Size: 630 314 360 1013 245 2579
Fragments arranged by size:
2579 1335 1215 1083 1080 1013 897 798
632 630 419 360 349 347 314 306
267 245 243 231 204 192 169 168
163 144 141 138 138 108 108 102
98 81 81 78 52 51 36 36
27 21 18 18 15 15
NaeI GCC'GGC
Cuts at: 1708 3135 3316 5733 6702 10231 14926 1708
Size: 1427 181 2417 969 3529 4695 3527
Fragments arranged by size:
4695 3529 3527 2417 1427 969 181
NarI GG'CG_CC
Cuts at: 2241 4374 8132 11247 14176 14424 2241
Size: 2133 3758 3115 2929 248 4562
Fragments arranged by size:
4562 3758 3115 2929 2133 248
NcoI C'CATG_G
Cuts at: 803 1106 2358 4709 5220 7499 9105 9932 13650
Size: 303 1252 2351 511 2279 1606 827 3718
Cuts at: 13650 803
Size: 3898
Fragments arranged by size:
3898 3718 2351 2279 1606 1252 827 511
303
NdeI CA'TA_TG
Cuts at: 622 622
Size: 16745
NgoMIV G'CCGG_C
Cuts at: 1706 3133 3314 5731 6700 10229 14924 1706
Size: 1427 181 2417 969 3529 4695 3527
Fragments arranged by size:
4695 3529 3527 2417 1427 969 181
NheI G'CTAG_C
Cuts at: 10114 10114
Size: 16745
NsiI A_TGCA'T
Cuts at: 3334 3334
Size: 16745
NspI r_CATG'y
Cuts at: 847 891 1049 5543 5724 6402 7854 9946 11397
Size: 44 158 4494 181 678 1452 2092 1451
Cuts at: 11397 12653 12867 13061 13798 14298 15205 847
Size: 1256 214 194 737 500 907 2387
Fragments arranged by size:
4494 2387 2092 1452 1451 1256 907 737
678 500 214 194 181 158 44
PasI CC'CwG_GG
Cuts at: 2057 2572 6120 7057 9342 9548 2057
Size: 515 3548 937 2285 206 9254
Fragments arranged by size:
9254 3548 2285 937 515 206
PciI A'CATG_T
Cuts at: 1045 6398 11393 15201 1045
Size: 5353 4995 3808 2589
Fragments arranged by size:
5353 4995 3808 2589
PflMI CCAn_nnn'nTGG
Cuts at: 971 1917 2083 2610 2840 2963 4625 4710 5094
Size: 946 166 527 230 123 1662 85 384
Cuts at: 5094 5918 7041 7989 8243 8357 12005 12155 13668
Size: 824 1123 948 254 114 3648 150 1513
Cuts at: 13668 971
Size: 4048
Fragments arranged by size:
4048 3648 1662 1513 1123 948 946 824
527 384 254 230 166 150 123 114
85
PfoI T'CCnGG_A
Cuts at: 392 1748 2708 2962 3866 5389 5863 6925 7640
Size: 1356 960 254 904 1523 474 1062 715
Cuts at: 7640 392
Size: 9497
Fragments arranged by size:
9497 1523 1356 1062 960 904 715 474
254
PmeI GTTT'AAAC
Cuts at: 13744 13744
Size: 16745
PmlI CAC'GTG
Cuts at: 951 7452 8463 12872 951
Size: 6501 1011 4409 4824
Fragments arranged by size:
6501 4824 4409 1011
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10828 10860 15884 15916 10828
Size: 32 5024 32 11657
Fragments arranged by size:
11657 5024 32 32
PpuMI rG'GwC_Cy
Cuts at: 1746 2115 2323 3864 4232 5867 6792 7578 7952
Size: 369 208 1541 368 1635 925 786 374
Cuts at: 7952 8979 9390 11712 13728 1746
Size: 1027 411 2322 2016 4763
Fragments arranged by size:
4763 2322 2016 1635 1541 1027 925 786
411 374 369 368 208
PshAI GACnn'nnGTC
Cuts at: 3412 5062 7583 13237 3412
Size: 1650 2521 5654 6920
Fragments arranged by size:
6920 5654 2521 1650
PspOMI G'GGCC_C
Cuts at: 421 496 755 1972 3751 4746 5334 12897 13321
Size: 75 259 1217 1779 995 588 7563 424
Cuts at: 13321 13707 421
Size: 386 3459
Fragments arranged by size:
7563 3459 1779 1217 995 588 424 386
259 75
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2632 2664 2632
Size: 32 16713
PstI C_TGCA'G
Cuts at: 211 589 653 2551 3142 3544 3685 4685 5073
Size: 378 64 1898 591 402 141 1000 388
Cuts at: 5073 5659 8026 8041 8965 9085 10012 11437 11650
Size: 586 2367 15 924 120 927 1425 213
Cuts at: 11650 12716 13252 13792 14477 211
Size: 1066 536 540 685 2479
Fragments arranged by size:
2479 2367 1898 1425 1066 1000 927 924
685 591 586 540 536 402 388 378
213 141 120 64 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 16745
PvuII CAG'CTG
Cuts at: 5463 5631 6263 6612 7535 8117 8536 9167 11459
Size: 168 632 349 923 582 419 631 2292
Cuts at: 11459 11651 12734 13226 14170 14530 5463
Size: 192 1083 492 944 360 7678
Fragments arranged by size:
7678 2292 1083 944 923 632 631 582
492 419 360 349 192 168
RsrII CG'GwC_CG
Cuts at: 2799 4784 14940 2799
Size: 1985 10156 4604
Fragments arranged by size:
10156 4604 1985
SacI G_AGCT'C
Cuts at: 440 3650 8725 13759 440
Size: 3210 5075 5034 3426
Fragments arranged by size:
5075 5034 3426 3210
SacII CC_GC'GG
Cuts at: 4077 9150 4077
Size: 5073 11672
SalI G'TCGA_C
Cuts at: 1192 13782 1192
Size: 12590 4155
SanDI GG'GwC_CC
Cuts at: 4232 13728 4232
Size: 9496 7249
SapI GCTCTTCn'nnn_
Cuts at: 3385 5323 8080 14768 14978 3385
Size: 1938 2757 6688 210 5152
Fragments arranged by size:
6688 5152 2757 1938 210
SbfI CC_TGCA'GG
Cuts at: 3544 4685 13792 3544
Size: 1141 9107 6497
Fragments arranged by size:
9107 6497 1141
ScaI AGT'ACT
Cuts at: 16262 16262
Size: 16745
SexAI A'CCwGG_T
Cuts at: 1232 1457 2603 11708 1232
Size: 225 1146 9105 6269
Fragments arranged by size:
9105 6269 1146 225
SfcI C'TryA_G
Cuts at: 33 207 585 649 2547 3138 3234 3540 3681
Size: 174 378 64 1898 591 96 306 141
Cuts at: 3681 3972 4157 4681 5069 5655 5736 8022 8037
Size: 291 185 524 388 586 81 2286 15
Cuts at: 8037 8151 8961 9081 10008 11433 11646 12712 13248
Size: 114 810 120 927 1425 213 1066 536
Cuts at: 13248 13788 14473 15466 15657 33
Size: 540 685 993 191 1121
Fragments arranged by size:
2286 1898 1425 1121 1066 993 927 810
685 591 586 540 536 524 388 378
306 291 213 191 185 174 141 120
114 96 81 64 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 5333 5342 5333
Size: 9 16736
SfoI GGC'GCC
Cuts at: 2242 4375 8133 11248 14177 14425 2242
Size: 2133 3758 3115 2929 248 4562
Fragments arranged by size:
4562 3758 3115 2929 2133 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 16745
SgrAI Cr'CCGG_yG
Cuts at: 11930 11930
Size: 16745
SmaI CCC'GGG
Cuts at: 1471 4297 6139 10035 10513 13767 1471
Size: 2826 1842 3896 478 3254 4449
Fragments arranged by size:
4449 3896 3254 2826 1842 478
SmlI C'TyrA_G
Cuts at: 874 6733 8983 9262 13900 14815 15307 15569 874
Size: 5859 2250 279 4638 915 492 262 2050
Fragments arranged by size:
5859 4638 2250 2050 915 492 279 262
SpeI A'CTAG_T
Cuts at: 67 4105 67
Size: 4038 12707
SphI G_CATG'C
Cuts at: 891 12867 13798 14298 891
Size: 11976 931 500 3338
Fragments arranged by size:
11976 3338 931 500
StuI AGG'CCT
Cuts at: 5761 6968 9407 11044 12254 12371 13565 5761
Size: 1207 2439 1637 1210 117 1194 8941
Fragments arranged by size:
8941 2439 1637 1210 1207 1194 117
StyI C'CwwG_G
Cuts at: 441 803 1082 1106 1742 2120 2358 4273 4709
Size: 362 279 24 636 378 238 1915 436
Cuts at: 4709 5220 7499 9105 9290 9932 10688 11630 12473
Size: 511 2279 1606 185 642 756 942 843
Cuts at: 12473 13452 13650 13733 13879 14233 441
Size: 979 198 83 146 354 2953
Fragments arranged by size:
2953 2279 1915 1606 979 942 843 756
642 636 511 436 378 362 354 279
238 198 185 146 83 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 828 2075 2241 2816 3175 3465 4063 4176 4286
Size: 1247 166 575 359 290 598 113 110
Cuts at: 4286 4681 5278 5910 7121 7407 8109 8127 8473
Size: 395 597 632 1211 286 702 18 346
Cuts at: 8473 9118 9355 9429 9433 9862 10591 11338 11971
Size: 645 237 74 4 429 729 747 633
Cuts at: 11971 11997 12046 12925 14328 14461 14920 15129 828
Size: 26 49 879 1403 133 459 209 2444
Fragments arranged by size:
2444 1403 1247 1211 879 747 729 702
645 633 632 598 597 575 459 429
395 359 346 290 286 237 209 166
133 113 110 74 49 26 18 4
TatI w'GTAC_w
Cuts at: 1955 2093 4994 5789 6305 8546 9455 9584 11717
Size: 138 2901 795 516 2241 909 129 2133
Cuts at: 11717 12023 12152 12284 16260 1955
Size: 306 129 132 3976 2440
Fragments arranged by size:
3976 2901 2440 2241 2133 909 795 516
306 138 132 129 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1551 1583 2137 2169 2382 2414 5253 5285 5488
Size: 32 554 32 213 32 2839 32 203
Cuts at: 5488 5520 5953 5985 11335 11367 16262 16294 1551
Size: 32 433 32 5350 32 4895 32 2002
Fragments arranged by size:
5350 4895 2839 2002 554 433 213 203
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1234 2623 4788 4954 5107 7744 9700 9835 11227
Size: 1389 2165 166 153 2637 1956 135 1392
Cuts at: 11227 12514 14542 1234
Size: 1287 2028 3437
Fragments arranged by size:
3437 2637 2165 2028 1956 1392 1389 1287
166 153 135
XbaI T'CTAG_A
Cuts at: 6126 13776 6126
Size: 7650 9095
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1870 2064 2084 5242 5919 6390 7449 8893 9555
Size: 194 20 3158 677 471 1059 1444 662
Cuts at: 9555 10071 10585 11836 12006 13252 13638 14240 1870
Size: 516 514 1251 170 1246 386 602 4375
Fragments arranged by size:
4375 3158 1444 1251 1246 1059 677 662
602 516 514 471 386 194 170 20
XmaI C'CCGG_G
Cuts at: 1469 4295 6137 10033 10511 13765 1469
Size: 2826 1842 3896 478 3254 4449
Fragments arranged by size:
4449 3896 3254 2826 1842 478
XmnI GAAnn'nnTTC
Cuts at: 3829 7813 8206 9194 3829
Size: 3984 393 988 11380
Fragments arranged by size:
11380 3984 988 393
ZraI GAC'GTC
Cuts at: 12396 12396
Size: 16745
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BsiWI BssHII BstZ17I EcoRV HpaI MfeI
NotI NruI PacI PsiI PspXI SnaBI SrfI SspI
SwaI XhoI