Restriction map for
pF1KE3911
(Circular) (Six-base) MAPSORT of: pF1KE3911.seq Check: 6315 from: 1 to: 5243
pF1KE3911 5243 bp
With 182 enzymes: SgfI *
November 15, 2013 14:41 ..
AccI GT'mk_AC
Cuts at: 1193 2281 1193
Size: 1088 4155
Acc65I G'GTAC_C
Cuts at: 2259 2259
Size: 5243
AclI AA'CG_TT
Cuts at: 5083 5083
Size: 5243
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 1408 1600 3068 3500 4247 4515 146
Size: 1262 192 1468 432 747 268 874
Fragments arranged by size:
1468 1262 874 747 432 268 192
AflIII A'CryG_T
Cuts at: 1148 1793 2595 3699 4676 1148
Size: 645 802 1104 977 1715
Fragments arranged by size:
1715 1104 977 802 645
AgeI A'CCGG_T
Cuts at: 3631 3631
Size: 5243
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 716 910 1330 716
Size: 194 420 4629
Fragments arranged by size:
4629 420 194
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 925 957 925
Size: 32 5211
AlwNI CAG_nnn'CTG
Cuts at: 1785 2548 4115 1785
Size: 763 1567 2913
Fragments arranged by size:
2913 1567 763
ApaLI G'TGCA_C
Cuts at: 1419 1966 4013 1419
Size: 547 2047 2649
Fragments arranged by size:
2649 2047 547
ApoI r'AATT_y
Cuts at: 291 1655 2247 4596 4852 291
Size: 1364 592 2349 256 682
Fragments arranged by size:
2349 1364 682 592 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5243
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5243
AvaI C'yCGr_G
Cuts at: 507 526 874 2263 507
Size: 19 348 1389 3487
Fragments arranged by size:
3487 1389 348 19
BamHI G'GATC_C
Cuts at: 2268 2268
Size: 5243
BanI G'GyrC_C
Cuts at: 1548 2259 2673 2921 2956 1548
Size: 711 414 248 35 3835
Fragments arranged by size:
3835 711 414 248 35
BanII G_rGCy'C
Cuts at: 410 480 638 1295 2012 2257 3287 410
Size: 70 158 657 717 245 1030 2366
Fragments arranged by size:
2366 1030 717 657 245 158 70
BbeI G_GCGC'C
Cuts at: 2677 2925 2677
Size: 248 4995
BbsI GAAGACnn'nnnn_
Cuts at: 719 814 719
Size: 95 5148
BbvCI CC'TCA_GC
Cuts at: 2186 2186
Size: 5243
BciVI GTATCCnnnnn_n'
Cuts at: 3141 3902 5243 3141
Size: 761 1341 3141
Fragments arranged by size:
3141 1341 761
BclI T'GATC_A
Cuts at: 162 1508 2763 162
Size: 1346 1255 2642
Fragments arranged by size:
2642 1346 1255
BglII A'GATC_T
Cuts at: 821 2117 2758 4795 821
Size: 1296 641 2037 1269
Fragments arranged by size:
2037 1296 1269 641
BlpI GC'TnA_GC
Cuts at: 1982 2355 1982
Size: 373 4870
Bme1580I G_kGCm'C
Cuts at: 1423 1553 1970 2868 2961 4017 1423
Size: 130 417 898 93 1056 2649
Fragments arranged by size:
2649 1056 898 417 130 93
BmgBI CAC'GTC
Cuts at: 1077 1149 1077
Size: 72 5171
BmrI ACTGGGnnnn_n'
Cuts at: 2455 2536 2871 5015 2455
Size: 81 335 2144 2683
Fragments arranged by size:
2683 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 516 827 3610 516
Size: 311 2783 2149
Fragments arranged by size:
2783 2149 311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 450 630 1126 1132 1234 1786 1834 2026 450
Size: 180 496 6 102 552 48 192 3667
Fragments arranged by size:
3667 552 496 192 180 102 48 6
Bpu10I CC'TnA_GC
Cuts at: 2186 2186
Size: 5243
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 831 919 1348 2134 2419 3298 3790 4088
Size: 613 88 429 786 285 879 492 298
Cuts at: 4088 218
Size: 1373
Fragments arranged by size:
1373 879 786 613 492 429 298 285
88
BsaI GGTCTCn'nnnn_
Cuts at: 106 4947 106
Size: 4841 402
BsaAI yAC'GTr
Cuts at: 1977 2596 1977
Size: 619 4624
BsaBI GATnn'nnATC
Cuts at: 6 2757 4794 6
Size: 2751 2037 455
Fragments arranged by size:
2751 2037 455
BsaHI Gr'CG_yC
Cuts at: 964 1478 1855 2674 2922 3624 5120 964
Size: 514 377 819 248 702 1496 1087
Fragments arranged by size:
1496 1087 819 702 514 377 248
BsaWI w'CCGG_w
Cuts at: 881 1118 2135 2432 2953 3631 3905 4052 4463
Size: 237 1017 297 521 678 274 147 411
Cuts at: 4463 881
Size: 1661
Fragments arranged by size:
1661 1017 678 521 411 297 274 237
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 925 955 925
Size: 30 5213
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 544 769 784 787 790 793 796 931
Size: 306 225 15 3 3 3 3 135
Cuts at: 931 1141 1314 1774 2128 238
Size: 210 173 460 354 3353
Fragments arranged by size:
3353 460 354 306 225 210 173 135
15 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2098 2217 2668 3426 4003 4499 2098
Size: 119 451 758 577 496 2842
Fragments arranged by size:
2842 758 577 496 451 119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 1326 2056 1018
Size: 308 730 4205
Fragments arranged by size:
4205 730 308
BsiEI CG_ry'CG
Cuts at: 104 2785 2831 4039 104
Size: 2681 46 1208 1308
Fragments arranged by size:
2681 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1118 1295 1423 1970 2012 2257 3035 3225 4017
Size: 177 128 547 42 245 778 190 792
Cuts at: 4017 1118
Size: 2344
Fragments arranged by size:
2344 792 778 547 245 190 177 128
42
BsmI GAATG_Cn'
Cuts at: 1514 1514
Size: 5243
Bsp1286I G_dGCh'C
Cuts at: 410 480 638 1118 1295 1423 1553 1970 2012
Size: 70 158 480 177 128 130 417 42
Cuts at: 2012 2257 2868 2961 3035 3225 3287 4017 410
Size: 245 611 93 74 190 62 730 1636
Fragments arranged by size:
1636 730 611 480 417 245 190 177
158 130 128 93 74 70 62 42
BspEI T'CCGG_A
Cuts at: 881 4463 881
Size: 3582 1661
BspHI T'CATG_A
Cuts at: 4419 4419
Size: 5243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 995 1688 2293 2507 2809 3190 995
Size: 693 605 214 302 381 3048
Fragments arranged by size:
3048 693 605 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1017 1095 1230 1413 3535 3589 5073 5238
Size: 967 78 135 183 2122 54 1484 165
Cuts at: 5238 50
Size: 55
Fragments arranged by size:
2122 1484 967 183 165 135 78 55
54
BsrDI GCAATG_nn'
Cuts at: 267 464 3155 267
Size: 197 2691 2355
Fragments arranged by size:
2691 2355 197
BsrFI r'CCGG_y
Cuts at: 537 786 1694 3241 3422 3631 537
Size: 249 908 1547 181 209 2149
Fragments arranged by size:
2149 1547 908 249 209 181
BssSI C'ACGA_G
Cuts at: 991 3514 3872 991
Size: 2523 358 2362
Fragments arranged by size:
2523 2362 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2620 2620
Size: 5243
BstBI TT'CG_AA
Cuts at: 3604 3604
Size: 5243
BstXI CCAn_nnnn'nTGG
Cuts at: 369 1071 369
Size: 702 4541
BstYI r'GATC_y
Cuts at: 1 821 867 878 902 1028 2054 2117 2132
Size: 820 46 11 24 126 1026 63 15
Cuts at: 2132 2268 2735 2758 3093 3339 4351 4437 4449
Size: 136 467 23 335 246 1012 86 12
Cuts at: 4449 4795 1
Size: 346 449
Fragments arranged by size:
1026 1012 820 467 449 346 335 246
136 126 86 63 46 24 23 15
12 11
Bsu36I CC'TnA_GG
Cuts at: 914 1630 914
Size: 716 4527
BtgI C'CryG_G
Cuts at: 107 1738 107
Size: 1631 3612
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3193 3374 3537 3193
Size: 181 163 4899
Fragments arranged by size:
4899 181 163
BtsI GCAGTG_nn'
Cuts at: 1421 2486 1421
Size: 1065 4178
DraI TTT'AAA
Cuts at: 2242 2242
Size: 5243
DrdI GACnn_nn'nnGTC
Cuts at: 2949 3807 2949
Size: 858 4385
EaeI y'GGCC_r
Cuts at: 535 545 734 788 1787 2828 3002 3393 3420
Size: 10 189 54 999 1041 174 391 27
Cuts at: 3420 4765 535
Size: 1345 1013
Fragments arranged by size:
1345 1041 1013 999 391 189 174 54
27 10
EagI C'GGCC_G
Cuts at: 2828 2828
Size: 5243
EarI CTCTTCn'nnn_
Cuts at: 761 1063 1384 1394 1651 1669 3266 3476 761
Size: 302 321 10 257 18 1597 210 2528
Fragments arranged by size:
2528 1597 321 302 257 210 18 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3451 3761 3907 5053 3451
Size: 310 146 1146 3641
Fragments arranged by size:
3641 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1293 2010 2255 1293
Size: 717 245 4281
Fragments arranged by size:
4281 717 245
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 450 630 1126 1132 1234 1408 1600 1786
Size: 304 180 496 6 102 174 192 186
Cuts at: 1786 1834 2026 3068 3500 4247 4515 146
Size: 48 192 1042 432 747 268 874
Fragments arranged by size:
1042 874 747 496 432 304 268 192
192 186 180 174 102 48 6
EcoNI CCTnn'n_nnAGG
Cuts at: 643 862 643
Size: 219 5024
EcoO109I rG'GnC_Cy
Cuts at: 573 728 1609 2382 573
Size: 155 881 773 3434
Fragments arranged by size:
3434 881 773 155
EcoRI G'AATT_C
Cuts at: 2247 2247
Size: 5243
EcoRV GAT'ATC
Cuts at: 249 249
Size: 5243
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1662 3262 1662
Size: 1600 3643
FseI GG_CCGG'CC
Cuts at: 541 541
Size: 5243
FspI TGC'GCA
Cuts at: 3024 3024
Size: 5243
HaeII r_GCGC'y
Cuts at: 2677 2925 3947 4926 2677
Size: 248 1022 979 2994
Fragments arranged by size:
2994 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1171 1203 2109 2141 3085 3115 3117 3147 1171
Size: 32 906 32 944 30 2 30 3267
Fragments arranged by size:
3267 944 906 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2282 2282
Size: 5243
KasI G'GCGC_C
Cuts at: 2673 2921 2673
Size: 248 4995
KpnI G_GTAC'C
Cuts at: 2263 2263
Size: 5243
MluI A'CGCG_T
Cuts at: 4676 4676
Size: 5243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1088 1861 3914 4098 1088
Size: 773 2053 184 2233
Fragments arranged by size:
2233 2053 773 184
MscI TGG'CCA
Cuts at: 1789 3004 1789
Size: 1215 4028
MslI CAynn'nnrTG
Cuts at: 1843 3232 3359 1843
Size: 1389 127 3727
Fragments arranged by size:
3727 1389 127
MspA1I CmG'CkG
Cuts at: 365 890 1245 1571 1740 2354 2668 3028 4041
Size: 525 355 326 169 614 314 360 1013
Cuts at: 4041 4286 365
Size: 245 1322
Fragments arranged by size:
1322 1013 614 525 360 355 326 314
245 169
NaeI GCC'GGC
Cuts at: 539 788 1696 3424 539
Size: 249 908 1728 2358
Fragments arranged by size:
2358 1728 908 249
NarI GG'CG_CC
Cuts at: 2674 2922 2674
Size: 248 4995
NcoI C'CATG_G
Cuts at: 107 107
Size: 5243
NgoMIV G'CCGG_C
Cuts at: 537 786 1694 3422 537
Size: 249 908 1728 2358
Fragments arranged by size:
2358 1728 908 249
NspI r_CATG'y
Cuts at: 1469 1848 2296 2796 3703 1469
Size: 379 448 500 907 3009
Fragments arranged by size:
3009 907 500 448 379
PciI A'CATG_T
Cuts at: 3699 3699
Size: 5243
PflMI CCAn_nnn'nTGG
Cuts at: 1005 1785 2175 1005
Size: 780 390 4073
Fragments arranged by size:
4073 780 390
PfoI T'CCnGG_A
Cuts at: 607 1070 1103 1250 1268 1621 1704 2030 607
Size: 463 33 147 18 353 83 326 3820
Fragments arranged by size:
3820 463 353 326 147 83 33 18
PmeI GTTT'AAAC
Cuts at: 2242 2242
Size: 5243
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 925 957 4382 4414 925
Size: 32 3425 32 1754
Fragments arranged by size:
3425 1754 32 32
PpuMI rG'GwC_Cy
Cuts at: 728 728
Size: 5243
PstI C_TGCA'G
Cuts at: 932 1598 2290 2975 932
Size: 666 692 685 3200
Fragments arranged by size:
3200 692 685 666
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5243
PvuII CAG'CTG
Cuts at: 365 1245 2668 3028 365
Size: 880 1423 360 2580
Fragments arranged by size:
2580 1423 880 360
RsrII CG'GwC_CG
Cuts at: 3438 3438
Size: 5243
SacI G_AGCT'C
Cuts at: 1295 2012 2257 1295
Size: 717 245 4281
Fragments arranged by size:
4281 717 245
SacII CC_GC'GG
Cuts at: 1741 1741
Size: 5243
SalI G'TCGA_C
Cuts at: 2280 2280
Size: 5243
SanDI GG'GwC_CC
Cuts at: 728 728
Size: 5243
SapI GCTCTTCn'nnn_
Cuts at: 1651 3266 3476 1651
Size: 1615 210 3418
Fragments arranged by size:
3418 1615 210
SbfI CC_TGCA'GG
Cuts at: 2290 2290
Size: 5243
ScaI AGT'ACT
Cuts at: 4760 4760
Size: 5243
SexAI A'CCwGG_T
Cuts at: 1718 1718
Size: 5243
SfcI C'TryA_G
Cuts at: 33 907 928 1594 2286 2971 3964 4155 33
Size: 874 21 666 692 685 993 191 1121
Fragments arranged by size:
1121 993 874 692 685 666 191 21
SfoI GGC'GCC
Cuts at: 2675 2923 2675
Size: 248 4995
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5243
SmaI CCC'GGG
Cuts at: 509 2265 509
Size: 1756 3487
SmlI C'TyrA_G
Cuts at: 233 846 898 1363 2149 2398 3313 3805 4067
Size: 613 52 465 786 249 915 492 262
Cuts at: 4067 233
Size: 1409
Fragments arranged by size:
1409 915 786 613 492 465 262 249
52
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5243
SphI G_CATG'C
Cuts at: 1469 2296 2796 1469
Size: 827 500 3916
Fragments arranged by size:
3916 827 500
StyI C'CwwG_G
Cuts at: 107 191 332 2377 2731 107
Size: 84 141 2045 354 2619
Fragments arranged by size:
2619 2045 354 141 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1985 2826 2959 3418 3627 1985
Size: 841 133 459 209 3601
Fragments arranged by size:
3601 841 459 209 133
TatI w'GTAC_w
Cuts at: 4758 4758
Size: 5243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4760 4792 4760
Size: 32 5211
Tth111I GACn'n_nGTC
Cuts at: 3040 3040
Size: 5243
XbaI T'CTAG_A
Cuts at: 2274 2274
Size: 5243
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 945 2738 945
Size: 1793 3450
XmaI C'CCGG_G
Cuts at: 507 2263 507
Size: 1756 3487
XmnI GAAnn'nnTTC
Cuts at: 1645 1645
Size: 5243
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmtI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI