Restriction map for
pF1KE3935
(Circular) (Six-base) MAPSORT of: pF1KE3935.seq Check: 6969 from: 1 to: 4511
pF1KE3935 4511 bp
With 182 enzymes: SgfI *
November 18, 2016 17:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 819 819
Size: 4511
AccI GT'mk_AC
Cuts at: 272 1549 272
Size: 1277 3234
Acc65I G'GTAC_C
Cuts at: 603 1527 603
Size: 924 3587
AclI AA'CG_TT
Cuts at: 439 4351 439
Size: 3912 599
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 483 575 596 2336 2768 3515 3783 483
Size: 92 21 1740 432 747 268 1211
Fragments arranged by size:
1740 1211 747 432 268 92 21
AflIII A'CryG_T
Cuts at: 1863 2967 3944 1863
Size: 1104 977 2430
Fragments arranged by size:
2430 1104 977
AgeI A'CCGG_T
Cuts at: 2899 2899
Size: 4511
AlwNI CAG_nnn'CTG
Cuts at: 694 730 811 910 1816 3383 694
Size: 36 81 99 906 1567 1822
Fragments arranged by size:
1822 1567 906 99 81 36
ApaLI G'TGCA_C
Cuts at: 3281 3281
Size: 4511
ApoI r'AATT_y
Cuts at: 148 475 1515 3864 4120 148
Size: 327 1040 2349 256 539
Fragments arranged by size:
2349 1040 539 327 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4511
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4511
AvaI C'yCGr_G
Cuts at: 1352 1409 1531 1352
Size: 57 122 4332
Fragments arranged by size:
4332 122 57
BamHI G'GATC_C
Cuts at: 1536 1536
Size: 4511
BanI G'GyrC_C
Cuts at: 603 1527 1941 2189 2224 603
Size: 924 414 248 35 2890
Fragments arranged by size:
2890 924 414 248 35
BanII G_rGCy'C
Cuts at: 1525 2555 1525
Size: 1030 3481
BbeI G_GCGC'C
Cuts at: 1945 2193 1945
Size: 248 4263
BbsI GAAGACnn'nnnn_
Cuts at: 845 1354 845
Size: 509 4002
BbvCI CC'TCA_GC
Cuts at: 998 998
Size: 4511
BciVI GTATCCnnnnn_n'
Cuts at: 908 2409 3170 4511 908
Size: 1501 761 1341 908
Fragments arranged by size:
1501 1341 908 761
BclI T'GATC_A
Cuts at: 630 2031 630
Size: 1401 3110
BglII A'GATC_T
Cuts at: 2026 4063 2026
Size: 2037 2474
BlpI GC'TnA_GC
Cuts at: 1623 1623
Size: 4511
Bme1580I G_kGCm'C
Cuts at: 2136 2229 3285 2136
Size: 93 1056 3362
Fragments arranged by size:
3362 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 884 920 1723 1804 2139 4283 884
Size: 36 803 81 335 2144 1112
Fragments arranged by size:
2144 1112 803 335 81 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 818 1457 2878 818
Size: 639 1421 2451
Fragments arranged by size:
2451 1421 639
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 788 1421 788
Size: 633 3878
Bpu10I CC'TnA_GC
Cuts at: 752 998 1291 752
Size: 246 293 3972
Fragments arranged by size:
3972 293 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 864 1687 2566 3058 3356 864
Size: 823 879 492 298 2019
Fragments arranged by size:
2019 879 823 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4215 4215
Size: 4511
BsaAI yAC'GTr
Cuts at: 1864 1864
Size: 4511
BsaBI GATnn'nnATC
Cuts at: 6 2025 4062 6
Size: 2019 2037 455
Fragments arranged by size:
2037 2019 455
BsaHI Gr'CG_yC
Cuts at: 1942 2190 2892 4388 1942
Size: 248 702 1496 2065
Fragments arranged by size:
2065 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1700 2221 2899 3173 3320 3731 1700
Size: 521 678 274 147 411 2480
Fragments arranged by size:
2480 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1442 1472 1442
Size: 30 4481
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 438 562 685 438
Size: 124 123 4264
Fragments arranged by size:
4264 124 123
BseYI C'CCAG_C
Cuts at: 1419 1936 2694 3271 3767 1419
Size: 517 758 577 496 2163
Fragments arranged by size:
2163 758 577 517 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1480 1480
Size: 4511
BsiEI CG_ry'CG
Cuts at: 104 1374 2053 2099 3307 104
Size: 1270 679 46 1208 1308
Fragments arranged by size:
1308 1270 1208 679 46
BsiHKAI G_wGCw'C
Cuts at: 1525 2303 2493 3285 1525
Size: 778 190 792 2751
Fragments arranged by size:
2751 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1525 2136 2229 2303 2493 2555 3285 1525
Size: 611 93 74 190 62 730 2751
Fragments arranged by size:
2751 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3731 3731
Size: 4511
BspHI T'CATG_A
Cuts at: 1152 3687 1152
Size: 2535 1976
BspMI ACCTGCnnnn'nnnn_
Cuts at: 819 1154 1494 1561 1775 2077 2458 819
Size: 335 340 67 214 302 381 2872
Fragments arranged by size:
2872 381 340 335 302 214 67
BsrBI CCG'CTC
Cuts at: 50 2803 2857 4341 4506 50
Size: 2753 54 1484 165 55
Fragments arranged by size:
2753 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2423 2423
Size: 4511
BsrFI r'CCGG_y
Cuts at: 2509 2690 2899 2509
Size: 181 209 4121
Fragments arranged by size:
4121 209 181
BssSI C'ACGA_G
Cuts at: 2782 3140 2782
Size: 358 4153
BstAPI GCAn_nnn'nTGC
Cuts at: 1888 1888
Size: 4511
BstBI TT'CG_AA
Cuts at: 2872 2872
Size: 4511
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4511
BstYI r'GATC_y
Cuts at: 1 1536 2003 2026 2361 2607 3619 3705 3717
Size: 1535 467 23 335 246 1012 86 12
Cuts at: 3717 4063 1
Size: 346 449
Fragments arranged by size:
1535 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 455 542 596 779 455
Size: 87 54 183 4187
Fragments arranged by size:
4187 183 87 54
BtgI C'CryG_G
Cuts at: 107 231 1446 1498 107
Size: 124 1215 52 3120
Fragments arranged by size:
3120 1215 124 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2461 2642 2805 2461
Size: 181 163 4167
Fragments arranged by size:
4167 181 163
BtsI GCAGTG_nn'
Cuts at: 169 1754 169
Size: 1585 2926
DraI TTT'AAA
Cuts at: 1510 1510
Size: 4511
DraIII CAC_nnn'GTG
Cuts at: 892 892
Size: 4511
DrdI GACnn_nn'nnGTC
Cuts at: 2217 3075 2217
Size: 858 3653
EaeI y'GGCC_r
Cuts at: 2096 2270 2661 2688 4033 2096
Size: 174 391 27 1345 2574
Fragments arranged by size:
2574 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2096 2096
Size: 4511
EarI CTCTTCn'nnn_
Cuts at: 225 787 1138 1334 2534 2744 225
Size: 562 351 196 1200 210 1992
Fragments arranged by size:
1992 1200 562 351 210 196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2719 3029 3175 4321 2719
Size: 310 146 1146 2909
Fragments arranged by size:
2909 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1523 1523
Size: 4511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 483 575 596 788 1421 2336 2768 3515 3783
Size: 92 21 192 633 915 432 747 268
Cuts at: 3783 483
Size: 1211
Fragments arranged by size:
1211 915 747 633 432 268 192 92
21
EcoO109I rG'GnC_Cy
Cuts at: 183 1051 1396 1405 1438 1650 183
Size: 868 345 9 33 212 3044
Fragments arranged by size:
3044 868 345 212 33 9
EcoRI G'AATT_C
Cuts at: 1515 1515
Size: 4511
EcoRV GAT'ATC
Cuts at: 495 495
Size: 4511
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 4511
FspI TGC'GCA
Cuts at: 1216 2292 1216
Size: 1076 3435
HaeII r_GCGC'y
Cuts at: 1945 2193 3215 4194 1945
Size: 248 1022 979 2262
Fragments arranged by size:
2262 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 906 938 1307 1339 2353 2383 2385 2415 906
Size: 32 369 32 1014 30 2 30 3002
Fragments arranged by size:
3002 1014 369 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1550 1550
Size: 4511
KasI G'GCGC_C
Cuts at: 1941 2189 1941
Size: 248 4263
KpnI G_GTAC'C
Cuts at: 607 1531 607
Size: 924 3587
MluI A'CGCG_T
Cuts at: 3944 3944
Size: 4511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3182 3366 3182
Size: 184 4327
MscI TGG'CCA
Cuts at: 2272 2272
Size: 4511
MslI CAynn'nnrTG
Cuts at: 112 347 2500 2627 112
Size: 235 2153 127 1996
Fragments arranged by size:
2153 1996 235 127
MspA1I CmG'CkG
Cuts at: 1002 1622 1936 2296 3309 3554 1002
Size: 620 314 360 1013 245 1959
Fragments arranged by size:
1959 1013 620 360 314 245
NaeI GCC'GGC
Cuts at: 2692 2692
Size: 4511
NarI GG'CG_CC
Cuts at: 1942 2190 1942
Size: 248 4263
NcoI C'CATG_G
Cuts at: 107 231 107
Size: 124 4387
NgoMIV G'CCGG_C
Cuts at: 2690 2690
Size: 4511
NspI r_CATG'y
Cuts at: 195 1564 2064 2971 195
Size: 1369 500 907 1735
Fragments arranged by size:
1735 1369 907 500
PciI A'CATG_T
Cuts at: 2967 2967
Size: 4511
PflMI CCAn_nnn'nTGG
Cuts at: 390 892 910 1474 390
Size: 502 18 564 3427
Fragments arranged by size:
3427 564 502 18
PmeI GTTT'AAAC
Cuts at: 1510 1510
Size: 4511
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3650 3682 3650
Size: 32 4479
PpuMI rG'GwC_Cy
Cuts at: 1051 1396 1051
Size: 345 4166
PspXI vC'TCGA_Gb
Cuts at: 1352 1409 1352
Size: 57 4454
PstI C_TGCA'G
Cuts at: 816 1558 2243 816
Size: 742 685 3084
Fragments arranged by size:
3084 742 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4511
PvuII CAG'CTG
Cuts at: 1936 2296 1936
Size: 360 4151
RsrII CG'GwC_CG
Cuts at: 2706 2706
Size: 4511
SacI G_AGCT'C
Cuts at: 1525 1525
Size: 4511
SalI G'TCGA_C
Cuts at: 1548 1548
Size: 4511
SanDI GG'GwC_CC
Cuts at: 1051 1051
Size: 4511
SapI GCTCTTCn'nnn_
Cuts at: 2534 2744 2534
Size: 210 4301
SbfI CC_TGCA'GG
Cuts at: 1558 1558
Size: 4511
ScaI AGT'ACT
Cuts at: 4028 4028
Size: 4511
SfcI C'TryA_G
Cuts at: 33 812 1554 2239 3232 3423 33
Size: 779 742 685 993 191 1121
Fragments arranged by size:
1121 993 779 742 685 191
SfoI GGC'GCC
Cuts at: 1943 2191 1943
Size: 248 4263
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4511
SmaI CCC'GGG
Cuts at: 1533 1533
Size: 4511
SmlI C'TyrA_G
Cuts at: 843 1352 1409 1666 2581 3073 3335 843
Size: 509 57 257 915 492 262 2019
Fragments arranged by size:
2019 915 509 492 262 257 57
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4511
SphI G_CATG'C
Cuts at: 1564 2064 1564
Size: 500 4011
SspI AAT'ATT
Cuts at: 1035 1035
Size: 4511
StyI C'CwwG_G
Cuts at: 107 231 1645 1999 107
Size: 124 1414 354 2619
Fragments arranged by size:
2619 1414 354 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 903 1388 1411 2094 2227 2686 2895 903
Size: 485 23 683 133 459 209 2519
Fragments arranged by size:
2519 683 485 459 209 133 23
TatI w'GTAC_w
Cuts at: 831 4026 831
Size: 3195 1316
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1190 1222 1442 1463 1474 1495 4028 4060 1190
Size: 32 220 21 11 21 2533 32 1641
Fragments arranged by size:
2533 1641 220 32 32 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 2308 2308
Size: 4511
XbaI T'CTAG_A
Cuts at: 1542 1542
Size: 4511
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1135 2006 1135
Size: 871 3640
XhoI C'TCGA_G
Cuts at: 1352 1409 1352
Size: 57 4454
XmaI C'CCGG_G
Cuts at: 1531 1531
Size: 4511
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI EcoNI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PshAI PsiI PspOMI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XmnI ZraI